                         SEQUENCE LISTING

<110>  BIOGEMMA
 
<120>  PRIME EDITING TECHNOLOGY FOR PLANT GENOME ENGINEERING

<130>  BRV 177 - WO

<150>  EP20305174.3
<151>  2020-02-21

<160>  110   

<170>  PatentIn version 3.5

<210>  1
<211>  514
<212>  PRT
<213>  Cauliflower mosaic virus

<400>  1

Ser Lys Thr Gln Gln Pro Glu Pro Val Asn Ile Ser Thr Asn Lys Ile 
1               5                   10                  15      


Glu Asn Pro Leu Glu Glu Ile Ala Ile Leu Ser Glu Gly Arg Arg Leu 
            20                  25                  30          


Ser Glu Glu Lys Leu Phe Ile Thr Gln Gln Arg Met Gln Lys Thr Glu 
        35                  40                  45              


Glu Leu Leu Glu Lys Val Cys Ser Glu Asn Pro Leu Asp Pro Asn Lys 
    50                  55                  60                  


Thr Lys Gln Trp Met Lys Ala Ser Ile Lys Leu Ser Asp Pro Ser Lys 
65                  70                  75                  80  


Ala Ile Lys Val Lys Pro Met Lys Tyr Ser Pro Met Asp Arg Glu Glu 
                85                  90                  95      


Phe Asp Lys Gln Ile Lys Glu Leu Leu Asp Leu Lys Val Ile Lys Pro 
            100                 105                 110         


Ser Lys Ser Pro His Met Ala Pro Ala Phe Leu Val Asn Asn Glu Ala 
        115                 120                 125             


Glu Asn Gly Arg Gly Asn Lys Arg Met Val Val Asn Tyr Lys Ala Met 
    130                 135                 140                 


Asn Lys Ala Thr Val Gly Asp Ala Tyr Asn Leu Pro Asn Lys Asp Glu 
145                 150                 155                 160 


Leu Leu Thr Leu Ile Arg Gly Lys Lys Ile Phe Ser Ser Phe Asp Cys 
                165                 170                 175     


Lys Ser Gly Phe Trp Gln Val Leu Leu Asp Gln Glu Ser Arg Pro Leu 
            180                 185                 190         


Thr Ala Phe Thr Cys Pro Gln Gly His Tyr Glu Trp Asn Val Val Pro 
        195                 200                 205             


Phe Gly Leu Lys Gln Ala Pro Ser Ile Phe Gln Arg His Met Asp Glu 
    210                 215                 220                 


Ala Phe Arg Val Phe Arg Lys Phe Cys Cys Val Tyr Val Asp Asp Ile 
225                 230                 235                 240 


Val Val Phe Ser Asn Asn Glu Glu Asp His Leu Leu His Val Ala Met 
                245                 250                 255     


Ile Leu Gln Lys Cys Asn Gln His Gly Ile Ile Leu Ser Lys Lys Lys 
            260                 265                 270         


Ala Gln Leu Phe Lys Lys Lys Ile Asn Phe Leu Gly Leu Glu Ile Asp 
        275                 280                 285             


Glu Gly Thr His Lys Pro Gln Gly His Ile Leu Glu His Ile Asn Lys 
    290                 295                 300                 


Phe Pro Asp Thr Leu Glu Asp Lys Lys Gln Leu Gln Arg Phe Leu Gly 
305                 310                 315                 320 


Ile Leu Thr Tyr Ala Ser Asp Tyr Ile Pro Asn Leu Ala Gln Met Arg 
                325                 330                 335     


Gln Pro Leu Gln Ala Lys Leu Lys Glu Asn Val Pro Trp Lys Trp Thr 
            340                 345                 350         


Lys Glu Asp Thr Leu Tyr Met Gln Lys Val Lys Lys Asn Leu Gln Gly 
        355                 360                 365             


Phe Pro Pro Leu His His Pro Leu Pro Glu Glu Lys Leu Ile Ile Glu 
    370                 375                 380                 


Thr Asp Ala Ser Asp Asp Tyr Trp Gly Gly Met Leu Lys Ala Ile Lys 
385                 390                 395                 400 


Ile Asn Glu Gly Thr Asn Thr Glu Leu Ile Cys Arg Tyr Arg Ser Gly 
                405                 410                 415     


Ser Phe Lys Ala Ala Glu Arg Asn Tyr His Ser Asn Asp Lys Glu Thr 
            420                 425                 430         


Leu Ala Val Ile Asn Thr Ile Lys Lys Phe Ser Ile Tyr Leu Thr Pro 
        435                 440                 445             


Val His Phe Leu Ile Arg Thr Asp Asn Thr His Phe Lys Ser Phe Val 
    450                 455                 460                 


Asn Leu Asn Tyr Lys Gly Asp Ser Lys Leu Gly Arg Asn Ile Arg Trp 
465                 470                 475                 480 


Gln Ala Trp Leu Ser His Tyr Ser Phe Asp Val Glu His Ile Lys Gly 
                485                 490                 495     


Thr Asp Asn His Phe Ala Asp Phe Leu Ser Arg Glu Phe Asn Lys Val 
            500                 505                 510         


Asn Ser 
        


<210>  2
<211>  498
<212>  PRT
<213>  Dahlia Mosaic virus

<400>  2

Ser Asn Thr Gln Pro Leu Pro Gly Thr Asn Ile Thr Lys Glu Ala Val 
1               5                   10                  15      


Glu Asp Glu Ser Asn Phe Ile Ile Asp Ile Ser Arg Tyr Gln Glu Ile 
            20                  25                  30          


Gln Asn Leu Leu Lys Lys Val Cys Ser Glu Asn Pro Ile Asp Pro Ala 
        35                  40                  45              


Lys Ser Lys Ala Trp Met Lys Ala Ser Ile Lys Leu Ala Asp Pro Lys 
    50                  55                  60                  


Ser Val Val Arg Val Lys Pro Met Val Tyr Ser Pro Glu Asp Arg Lys 
65                  70                  75                  80  


Glu Phe Glu Ile Gln Ile Lys Glu Leu Leu Asp Leu Lys Val Ile Glu 
                85                  90                  95      


Pro Ser Lys Ser Gln His Met Ser Pro Ala Phe Leu Val Glu Lys Glu 
            100                 105                 110         


Ala Glu Lys Gly Arg Gly Lys Lys Arg Met Val Val Asn Tyr Lys Lys 
        115                 120                 125             


Leu Asn Glu Val Thr Ile Gly Asp Ser His Asn Leu Pro Asn Met Gln 
    130                 135                 140                 


Glu Leu Ile Thr Leu Leu Arg Gly Lys Thr Ile Phe Ser Ser Phe Asp 
145                 150                 155                 160 


Cys Lys Ser Gly Phe Trp Gln Val Phe Leu Asp Gln Glu Ser Gln Lys 
                165                 170                 175     


Leu Thr Ala Phe Thr Cys Pro Gln Gly His Phe Gln Trp Arg Val Val 
            180                 185                 190         


Pro Phe Gly Leu Lys Gln Ala Pro Ser Ile Phe Gln Arg His Met Gln 
        195                 200                 205             


Asn Ala Leu Arg Gly Leu Glu Glu Phe Cys Leu Val Tyr Val Asp Asp 
    210                 215                 220                 


Ile Ile Val Phe Ser Asp Lys Glu Glu Glu His Tyr Thr His Val Leu 
225                 230                 235                 240 


Lys Val Leu Lys Arg Ile Glu Ser Leu Gly Ile Ile Leu Ser Glu Lys 
                245                 250                 255     


Lys Ala Asn Leu Phe Lys Glu Lys Ile Asn Phe Leu Gly Leu Glu Ile 
            260                 265                 270         


Asp Arg Gly Thr His Thr Pro Gln Asn His Ile Leu Glu His Leu His 
        275                 280                 285             


Asn Phe Pro Asp Arg Leu Glu Asp Lys Lys Gln Leu Gln Arg Phe Leu 
    290                 295                 300                 


Gly Val Leu Thr Tyr Ala Asp Ser Tyr Ile Pro Lys Leu Ala Glu Lys 
305                 310                 315                 320 


Arg Lys Pro Leu Gln Val Lys Leu Lys Lys Asp Gln Val Trp Ile Trp 
                325                 330                 335     


Asn Gln Ser Asp Thr Asp Tyr Val Lys Lys Ile Lys Lys Gly Leu Val 
            340                 345                 350         


Asn Phe Pro Lys Leu Tyr Leu Pro Lys Lys Glu Asp Ser Leu Ile Ile 
        355                 360                 365             


Glu Thr Asp Ala Ser Asp His Phe Trp Gly Gly Val Leu Lys Ala Gln 
    370                 375                 380                 


Thr Thr Glu Gly Asn Glu Leu Ile Cys Arg Tyr Ser Ser Gly Thr Phe 
385                 390                 395                 400 


Lys Pro Ala Glu Leu Asn Tyr His Ser Asn Glu Lys Glu Leu Leu Ala 
                405                 410                 415     


Val Lys Gln Val Ile Thr Lys Phe Ser Ile Tyr Leu Thr Pro Val Thr 
            420                 425                 430         


Phe Thr Val Arg Thr Asp Asn Val Asn Leu Leu Lys Arg Phe Met Asn 
        435                 440                 445             


Thr Lys Ile Thr Gly Asp Ser Lys Gln Gly Arg Leu Ile Arg Trp Gln 
    450                 455                 460                 


Met Trp Leu Ser His Tyr Thr Phe Asn Val Asn His Leu Lys Gly Glu 
465                 470                 475                 480 


Lys Asn Val Leu Ala Asp Tyr Leu Thr Arg Glu Tyr Gln Gln Lys Arg 
                485                 490                 495     


Gln Gln 
        


<210>  3
<211>  595
<212>  PRT
<213>  Arabidopsis thaliana

<400>  3

Ala Gln Ser Leu Ser Thr Pro Ala Gln Ser Ser Ser Ser Ser Pro Ser 
1               5                   10                  15      


Pro Thr Thr Ser Ala Ser Ser Ser Ser Thr Ser Pro Thr Pro Pro Ser 
            20                  25                  30          


Ile Leu Ile His Pro Pro Pro Pro Leu Ala Gln Ile Val Asn Asn Asn 
        35                  40                  45              


Asn Gln Ala Pro Leu Asn Thr His Ser Met Gly Thr Arg Ala Lys Ala 
    50                  55                  60                  


Gly Ile Ile Lys Pro Asn Pro Lys Tyr Ser Leu Ala Val Ser Leu Ala 
65                  70                  75                  80  


Ala Glu Ser Glu Pro Arg Thr Ala Ile Gln Ala Leu Lys Asp Glu Arg 
                85                  90                  95      


Trp Arg Asn Ala Met Gly Ser Glu Ile Asn Ala Gln Ile Gly Asn His 
            100                 105                 110         


Thr Trp Asp Leu Val Pro Pro Pro Pro Ser His Val Thr Ile Val Gly 
        115                 120                 125             


Cys Arg Trp Ile Phe Thr Lys Lys Tyr Asn Ser Asp Gly Ser Leu Asn 
    130                 135                 140                 


Arg Tyr Lys Ala Arg Leu Val Ala Lys Gly Tyr Asn Gln Arg Pro Gly 
145                 150                 155                 160 


Leu Asp Tyr Ala Glu Thr Phe Ser Pro Val Ile Lys Ser Thr Ser Ile 
                165                 170                 175     


Arg Ile Val Leu Gly Val Ala Val Asp Arg Ser Trp Pro Ile Arg Gln 
            180                 185                 190         


Leu Asp Val Asn Asn Ala Phe Leu Gln Gly Thr Leu Thr Asp Asp Val 
        195                 200                 205             


Tyr Met Ser Gln Pro Pro Gly Phe Ile Asp Lys Asp Arg Pro Asn Tyr 
    210                 215                 220                 


Val Cys Lys Leu Arg Lys Ala Leu Tyr Gly Leu Lys Gln Ala Pro Arg 
225                 230                 235                 240 


Ala Trp Tyr Val Glu Leu Arg Asn Tyr Leu Leu Thr Ile Gly Phe Val 
                245                 250                 255     


Asn Ser Val Ser Asp Thr Ser Leu Phe Val Leu Gln Arg Gly Lys Ser 
            260                 265                 270         


Ile Val Tyr Met Leu Val Tyr Val Asp Asp Ile Leu Ile Thr Gly Asn 
        275                 280                 285             


Asp Pro Thr Leu Leu His Asn Thr Leu Asp Asn Leu Ser Gln Arg Phe 
    290                 295                 300                 


Ser Val Lys Asp His Glu Glu Leu His Tyr Phe Leu Gly Ile Glu Ala 
305                 310                 315                 320 


Lys Arg Val Pro Thr Gly Leu His Leu Ser Gln Arg Arg Tyr Ile Leu 
                325                 330                 335     


Asp Leu Leu Ala Arg Thr Asn Met Ile Thr Ala Lys Pro Val Thr Thr 
            340                 345                 350         


Pro Met Ala Pro Ser Pro Lys Leu Ser Leu Tyr Ser Gly Thr Lys Leu 
        355                 360                 365             


Thr Asp Pro Thr Glu Tyr Arg Gly Ile Val Gly Ser Leu Gln Tyr Leu 
    370                 375                 380                 


Ala Phe Thr Arg Pro Asp Ile Ser Tyr Ala Val Asn Arg Leu Ser Gln 
385                 390                 395                 400 


Phe Met His Met Pro Thr Glu Glu His Leu Gln Ala Leu Lys Arg Ile 
                405                 410                 415     


Leu Arg Tyr Leu Ala Gly Thr Pro Asn His Gly Ile Phe Leu Lys Lys 
            420                 425                 430         


Gly Asn Thr Leu Ser Leu His Ala Tyr Ser Asp Ala Asp Trp Ala Gly 
        435                 440                 445             


Asp Lys Asp Asp Tyr Val Ser Thr Asn Gly Tyr Ile Val Tyr Leu Gly 
    450                 455                 460                 


His His Pro Ile Ser Trp Ser Ser Lys Lys Gln Lys Gly Val Val Arg 
465                 470                 475                 480 


Ser Ser Thr Glu Ala Glu Tyr Arg Ser Val Ala Asn Thr Ser Ser Glu 
                485                 490                 495     


Met Gln Trp Ile Cys Ser Leu Leu Thr Glu Leu Gly Ile Arg Leu Thr 
            500                 505                 510         


Arg Pro Pro Val Ile Tyr Cys Asp Asn Val Gly Ala Thr Tyr Leu Cys 
        515                 520                 525             


Ala Asn Pro Val Phe His Ser Arg Met Lys His Ile Ala Ile Asp Tyr 
    530                 535                 540                 


His Phe Ile Arg Asn Gln Val Gln Ser Gly Ala Leu Arg Val Val His 
545                 550                 555                 560 


Val Ser Thr His Asp Gln Leu Ala Asp Thr Leu Thr Lys Pro Leu Ser 
                565                 570                 575     


Arg Thr Ala Phe Gln Asn Phe Ala Ser Lys Ile Gly Val Thr Arg Val 
            580                 585                 590         


Pro Pro Ser 
        595 


<210>  4
<211>  606
<212>  PRT
<213>  Arabidopsis thaliana

<400>  4

Asn Gln Asn Ser Pro Leu Pro Gln Ser Pro Ile Ser Ser Pro His Ile 
1               5                   10                  15      


Pro Thr Pro Ser Thr Ser Ile Ser Glu Pro Asn Ser Pro Ser Ser Ser 
            20                  25                  30          


Ser Thr Ser Thr Pro Pro Leu Pro Pro Val Leu Pro Ala Pro Pro Ile 
        35                  40                  45              


Ile Gln Val Asn Ala Gln Ala Pro Val Asn Thr His Ser Met Ala Thr 
    50                  55                  60                  


Arg Ala Lys Asp Gly Ile Arg Lys Pro Asn Gln Lys Tyr Ser Tyr Ala 
65                  70                  75                  80  


Thr Ser Leu Ala Ala Asn Ser Glu Pro Arg Thr Ala Ile Gln Ala Met 
                85                  90                  95      


Lys Asp Asp Arg Trp Arg Gln Ala Met Gly Ser Glu Ile Asn Ala Gln 
            100                 105                 110         


Ile Gly Asn His Thr Trp Asp Leu Val Pro Pro Pro Pro Pro Ser Val 
        115                 120                 125             


Thr Ile Val Gly Cys Arg Trp Ile Phe Thr Lys Lys Phe Asn Ser Asp 
    130                 135                 140                 


Gly Ser Leu Asn Arg Tyr Lys Ala Arg Leu Val Ala Lys Gly Tyr Asn 
145                 150                 155                 160 


Gln Arg Pro Gly Leu Asp Tyr Ala Glu Thr Phe Ser Pro Val Ile Lys 
                165                 170                 175     


Ser Thr Ser Ile Arg Ile Val Leu Gly Val Ala Val Asp Arg Ser Trp 
            180                 185                 190         


Pro Ile Arg Gln Leu Asp Val Asn Asn Ala Phe Leu Gln Gly Thr Leu 
        195                 200                 205             


Thr Asp Glu Val Tyr Met Ser Gln Pro Pro Gly Phe Val Asp Lys Asp 
    210                 215                 220                 


Arg Pro Asp Tyr Val Cys Arg Leu Arg Lys Ala Ile Tyr Gly Leu Lys 
225                 230                 235                 240 


Gln Ala Pro Arg Ala Trp Tyr Val Glu Leu Arg Thr Tyr Leu Leu Thr 
                245                 250                 255     


Val Gly Phe Val Asn Ser Ile Ser Asp Thr Ser Leu Phe Val Leu Gln 
            260                 265                 270         


Arg Gly Arg Ser Ile Ile Tyr Met Leu Val Tyr Val Asp Asp Ile Leu 
        275                 280                 285             


Ile Thr Gly Asn Asp Thr Val Leu Leu Lys His Thr Leu Asp Ala Leu 
    290                 295                 300                 


Ser Gln Arg Phe Ser Val Lys Glu His Glu Asp Leu His Tyr Phe Leu 
305                 310                 315                 320 


Gly Ile Glu Ala Lys Arg Val Pro Gln Gly Leu His Leu Ser Gln Arg 
                325                 330                 335     


Arg Tyr Thr Leu Asp Leu Leu Ala Arg Thr Asn Met Leu Thr Ala Lys 
            340                 345                 350         


Pro Val Ala Thr Pro Met Ala Thr Ser Pro Lys Leu Thr Leu His Ser 
        355                 360                 365             


Gly Thr Lys Leu Pro Asp Pro Thr Glu Tyr Arg Gly Ile Val Gly Ser 
    370                 375                 380                 


Leu Gln Tyr Leu Ala Phe Thr Arg Pro Asp Leu Ser Tyr Ala Val Asn 
385                 390                 395                 400 


Arg Leu Ser Gln Tyr Met His Met Pro Thr Asp Asp His Trp Asn Ala 
                405                 410                 415     


Leu Lys Arg Val Leu Arg Tyr Leu Ala Gly Thr Pro Asp His Gly Ile 
            420                 425                 430         


Phe Leu Lys Lys Gly Asn Thr Leu Ser Leu His Ala Tyr Ser Asp Ala 
        435                 440                 445             


Asp Trp Ala Gly Asp Thr Asp Asp Tyr Val Ser Thr Asn Gly Tyr Ile 
    450                 455                 460                 


Val Tyr Leu Gly His His Pro Ile Ser Trp Ser Ser Lys Lys Gln Lys 
465                 470                 475                 480 


Gly Val Val Arg Ser Ser Thr Glu Ala Glu Tyr Arg Ser Val Ala Asn 
                485                 490                 495     


Thr Ser Ser Glu Leu Gln Trp Ile Cys Ser Leu Leu Thr Glu Leu Gly 
            500                 505                 510         


Ile Gln Leu Ser His Pro Pro Val Ile Tyr Cys Asp Asn Val Gly Ala 
        515                 520                 525             


Thr Tyr Leu Cys Ala Asn Pro Val Phe His Ser Arg Met Lys His Ile 
    530                 535                 540                 


Ala Leu Asp Tyr His Phe Ile Arg Asn Gln Val Gln Ser Gly Ala Leu 
545                 550                 555                 560 


Arg Val Val His Val Ser Thr His Asp Gln Leu Ala Asp Thr Leu Thr 
                565                 570                 575     


Lys Pro Leu Ser Arg Val Ala Phe Gln Asn Phe Ser Arg Lys Ile Gly 
            580                 585                 590         


Val Ile Lys Val Pro Pro Ser Cys Gly Gly Val Leu Arg Ile 
        595                 600                 605     


<210>  5
<211>  600
<212>  PRT
<213>  Oryza sativa

<400>  5

Ser Glu Met Ser Pro Phe Pro Phe His Tyr Ser Arg Arg Pro Arg Thr 
1               5                   10                  15      


Pro His Glu Ser Gln Pro Pro Ser Cys Leu Pro Ser Asp Val Pro Thr 
            20                  25                  30          


Ser Thr Ile Asn Ala Gln Ser Asp Asp Pro Pro Val Ala Pro Leu Ile 
        35                  40                  45              


Asp Asp Pro Leu Val Ser Ser Pro Gln Ser Ala Thr Pro Arg Tyr Gly 
    50                  55                  60                  


Leu Arg Asp Arg Ser Lys Ile Met Pro His Pro Arg Tyr Met Cys Ala 
65                  70                  75                  80  


Ala Ile Asp Gly Val Gln Glu Pro Ser Thr Tyr Gln Glu Ala Ala Ala 
                85                  90                  95      


Ile Pro Glu Trp Gln Leu Ala Met Thr Glu Glu Leu Ala Ala Leu Glu 
            100                 105                 110         


Arg Thr Arg Thr Trp Asp Ile Val Pro Leu Pro Cys His Ala Ile Pro 
        115                 120                 125             


Ile Thr Cys Lys Trp Val Phe Lys Val Lys Thr Arg Ser Asp Gly Ser 
    130                 135                 140                 


Ile Glu Arg Tyr Lys Ala Arg Leu Val Ala Arg Gly Phe Gln Gln Glu 
145                 150                 155                 160 


His Gly Arg Asp Tyr Asp Glu Thr Phe Ala Pro Val Ala His Met Thr 
                165                 170                 175     


Thr Val Arg Thr Leu Val Ala Val Ala Ala Ala Arg Ser Trp Thr Ile 
            180                 185                 190         


Ser Gln Met Asp Val Lys Asn Ala Phe Leu His Gly Asp Leu Lys Glu 
        195                 200                 205             


Glu Val Tyr Met Lys Pro Pro Pro Gly Val Asp Ala Pro Ala Asn Tyr 
    210                 215                 220                 


Val Phe Arg Leu Asn Arg Ala Leu Tyr Gly Leu Lys Gln Ala Pro Arg 
225                 230                 235                 240 


Ala Trp Phe Glu Arg Phe Ser Ser Val Val Cys Ala Ala Gly Phe Ser 
                245                 250                 255     


Pro Ser Asp His Asp Pro Ala Leu Phe Ile His Val Ser Asp His Gly 
            260                 265                 270         


Arg Thr Leu Leu Leu Leu Tyr Val Asp Asp Met Leu Ile Thr Gly Asp 
        275                 280                 285             


Asp Leu Glu Tyr Ile Ala Phe Val Lys Gln Cys Leu Ser Glu Gln Phe 
    290                 295                 300                 


Met Met Ser Asp Leu Gly Pro Leu Ser Tyr Phe Leu Gly Ile Glu Val 
305                 310                 315                 320 


Thr Asn Thr Glu Asp Gly Tyr Tyr Leu Ser Gln His Arg Tyr Ile Asp 
                325                 330                 335     


Asp Leu Ile Thr Arg Ser Gly Leu Thr Asp Thr Lys Thr Ala Thr Thr 
            340                 345                 350         


Pro Met Glu Leu His Val Gln Leu Arg Ser Thr Glu Gly Val Leu Leu 
        355                 360                 365             


Glu Asp Pro Ser Arg Tyr Arg Gln Ile Val Gly Ser Leu Val Tyr Leu 
    370                 375                 380                 


Thr Ile Thr Arg Pro Asp Ile Ala His Ile Val His Val Leu Ser Gln 
385                 390                 395                 400 


Phe Val Ser Ala Pro Thr Ser Val His Tyr Ser His Leu Leu Arg Val 
                405                 410                 415     


Leu Arg Tyr Leu Arg Gly Thr Ala Ser Arg Gln Leu Phe Tyr Ala Arg 
            420                 425                 430         


Ser Ser Gln Leu His Leu His Ala Tyr Ser Asp Ser Thr Trp Ala Ser 
        435                 440                 445             


Asp Pro Met Asp Arg Arg Ser Ile Thr Gly Tyr Cys Ile Phe Leu Gly 
    450                 455                 460                 


Thr Ser Leu Ile Ala Trp Lys Ser Lys Lys Gln Thr Ala Val Ser Arg 
465                 470                 475                 480 


Ser Ser Thr Glu Ser Glu Leu Arg Ala Leu Ala Thr Thr Thr Ser Glu 
                485                 490                 495     


Ile Val Trp Leu Arg Trp Leu Leu Ser Asp Leu Gly Val Glu Cys Lys 
            500                 505                 510         


Asp Pro Thr Pro Leu Leu Cys Asp Asn Thr Gly Ala Ile Gln Ile Ala 
        515                 520                 525             


Asn Asp Pro Val Lys His Glu Leu Thr Lys His Ile Gly Val Asp Ala 
    530                 535                 540                 


Ser Phe Thr Arg Ser His Cys Gln Gln Ser Thr Ile Ser Leu Arg Tyr 
545                 550                 555                 560 


Val Pro Ser Glu Leu Gln Val Ala Asp Phe Phe Thr Lys Ala Gln Thr 
                565                 570                 575     


Arg Glu Gln His Arg Leu Tyr Ile Leu Lys Leu Asn Val Ile Asp Pro 
            580                 585                 590         


Pro Asn His Ser Pro Asn Pro Pro 
        595                 600 


<210>  6
<211>  517
<212>  PRT
<213>  Oryza sativa

<400>  6

Leu Lys Gly Ser Cys Phe Ile Ala Thr Lys Ser Asp Leu Asp Glu Val 
1               5                   10                  15      


Asp Thr Asp Thr Val Phe Cys Tyr Ala Leu Val Cys Lys Glu Thr Leu 
            20                  25                  30          


Phe Pro Ile Glu Asp Thr Pro Ile Ser Leu Arg Pro Pro Val Thr Asn 
        35                  40                  45              


Leu Leu Gln Glu Tyr Ala Asp Ile Phe Pro Lys Glu Val Pro Pro Gly 
    50                  55                  60                  


Leu Pro Pro Ile Arg Gly Ile Glu His Gln Ile Asp Leu Ile Pro Gly 
65                  70                  75                  80  


Ala Ser Leu Pro Asn Arg Ala Pro Tyr Arg Thr Asn Pro Glu Glu Thr 
                85                  90                  95      


Lys Glu Ile Gln Arg Gln Val Gln Glu Leu Leu Asp Lys Gly Tyr Val 
            100                 105                 110         


Arg Glu Ser Leu Ser Pro Cys Ser Val Pro Val Leu Leu Val Pro Lys 
        115                 120                 125             


Lys Asp Gly Ser Trp Arg Met Cys Val Asp Cys Arg Ala Ile Asn Asn 
    130                 135                 140                 


Ile Thr Ile Arg Tyr Arg His Pro Ile Pro Arg Leu Asp Asp Met Leu 
145                 150                 155                 160 


Asp Glu Leu Ser Gly Ser Leu Val Phe Ser Lys Ile Asp Leu Arg Ser 
                165                 170                 175     


Gly Tyr His Gln Ile Arg Met Lys Leu Gly Asp Glu Trp Lys Thr Ala 
            180                 185                 190         


Phe Lys Thr Lys Phe Gly Leu Tyr Glu Trp Leu Val Met Pro Phe Gly 
        195                 200                 205             


Leu Thr Asn Ala Pro Ser Thr Phe Met Arg Leu Met Asn Glu Val Leu 
    210                 215                 220                 


Arg Ala Phe Ile Gly Arg Phe Val Val Val Tyr Phe Asp Asp Ile Leu 
225                 230                 235                 240 


Ile Tyr Ser Arg Ser Ile Glu Asp His His Gly His Leu Arg Ala Val 
                245                 250                 255     


Phe Asp Ala Leu Arg Asp Ala Arg Leu Phe Gly Asn Leu Glu Lys Cys 
            260                 265                 270         


Thr Phe Cys Thr Asp Arg Val Ser Phe Leu Gly Tyr Val Val Thr Pro 
        275                 280                 285             


Gln Gly Ile Glu Val Asp Gln Ala Lys Val Glu Ala Ile His Ser Trp 
    290                 295                 300                 


Pro Val Pro Thr Thr Ile Thr Gln Val Arg Ser Phe Leu Gly Leu Ala 
305                 310                 315                 320 


Gly Phe Tyr Arg Arg Phe Val Lys Asp Phe Ser Thr Ile Ala Ala Pro 
                325                 330                 335     


Leu His Glu Leu Thr Lys Arg Asn Val Thr Phe Thr Trp Ala Ala Ala 
            340                 345                 350         


Gln Arg Asn Ala Phe Asp Thr Leu Lys Asp Lys Leu Thr His Ala Pro 
        355                 360                 365             


Leu Leu Gln Leu Pro Asp Phe Asn Lys Thr Phe Glu Leu Glu Cys Asp 
    370                 375                 380                 


Ala Ser Gly Ile Gly Leu Gly Gly Val Leu Leu Gln Glu Gly Lys Pro 
385                 390                 395                 400 


Val Ala Tyr Phe Ser Glu Lys Leu Ser Gly Pro Ser Leu Asn Tyr Ser 
                405                 410                 415     


Thr Tyr Asp Lys Glu Leu Phe Ala Leu Val Arg Thr Leu Glu Thr Trp 
            420                 425                 430         


Gln His Tyr Leu Trp Pro Lys Glu Phe Val Ile His Ser Asp His Glu 
        435                 440                 445             


Ser Leu Lys His Ile Arg Ser Gln Ala Lys His Asn Arg Arg His Ala 
    450                 455                 460                 


Lys Trp Val Glu Phe Ile Glu Ser Phe Pro Tyr Val Ile Lys His Lys 
465                 470                 475                 480 


Lys Gly Lys Glu Asn Val Ile Ala Asp Ala Leu Ser Arg Arg Tyr Ala 
                485                 490                 495     


Met Leu Ser Gln Leu Asp Phe Lys Ile Phe Gly Leu Glu Thr Ile Lys 
            500                 505                 510         


Glu Gln Tyr Ala His 
        515         


<210>  7
<211>  620
<212>  PRT
<213>  Nicotiana tabacum

<400>  7

Val Arg Thr Ala Ala Asp Met Ser Glu Lys Val Lys Asn Gly Ile Ile 
1               5                   10                  15      


Pro Asn Phe Val Thr Ile Pro Ser Thr Ser Asn Asn Pro Thr Ser Ala 
            20                  25                  30          


Glu Ser Thr Thr Asp Glu Val Ser Glu Gln Gly Glu Gln Pro Gly Glu 
        35                  40                  45              


Val Ile Glu Gln Gly Glu Gln Leu Asp Glu Gly Val Glu Glu Val Glu 
    50                  55                  60                  


His Pro Thr Gln Gly Glu Glu Gln His Gln Pro Leu Arg Arg Ser Glu 
65                  70                  75                  80  


Arg Pro Arg Val Glu Ser Arg Arg Tyr Pro Ser Thr Glu Tyr Val Leu 
                85                  90                  95      


Ile Ser Asp Asp Arg Glu Pro Glu Ser Leu Lys Glu Val Leu Ser His 
            100                 105                 110         


Pro Glu Lys Asn Gln Leu Met Lys Ala Met Gln Glu Glu Met Glu Ser 
        115                 120                 125             


Leu Gln Lys Asn Gly Thr Tyr Lys Leu Val Glu Leu Pro Lys Gly Lys 
    130                 135                 140                 


Arg Pro Leu Lys Cys Lys Trp Val Phe Lys Leu Lys Lys Asp Gly Asp 
145                 150                 155                 160 


Cys Lys Leu Val Arg Tyr Lys Ala Arg Leu Val Val Lys Gly Phe Glu 
                165                 170                 175     


Gln Lys Lys Gly Ile Asp Phe Asp Glu Ile Phe Ser Pro Val Val Lys 
            180                 185                 190         


Met Thr Ser Ile Arg Thr Ile Leu Ser Leu Ala Ala Ser Leu Asp Leu 
        195                 200                 205             


Glu Val Glu Gln Leu Asp Val Lys Thr Ala Phe Leu His Gly Asp Leu 
    210                 215                 220                 


Glu Glu Glu Ile Tyr Met Glu Gln Pro Glu Gly Phe Glu Val Ala Gly 
225                 230                 235                 240 


Lys Lys His Met Val Cys Lys Leu Asn Lys Ser Leu Tyr Gly Leu Lys 
                245                 250                 255     


Gln Ala Pro Arg Gln Trp Tyr Met Lys Phe Asp Ser Phe Met Lys Ser 
            260                 265                 270         


Gln Thr Tyr Leu Lys Thr Tyr Ser Asp Pro Cys Val Tyr Phe Lys Arg 
        275                 280                 285             


Phe Ser Glu Asn Asn Phe Ile Ile Leu Leu Leu Tyr Val Asp Asp Met 
    290                 295                 300                 


Leu Ile Val Gly Lys Asp Lys Gly Leu Ile Ala Lys Leu Lys Gly Asp 
305                 310                 315                 320 


Leu Ser Lys Ser Phe Asp Met Lys Asp Leu Gly Pro Ala Gln Gln Ile 
                325                 330                 335     


Leu Gly Met Lys Ile Val Arg Glu Arg Thr Ser Arg Lys Leu Trp Leu 
            340                 345                 350         


Ser Gln Glu Lys Tyr Ile Glu Arg Val Leu Glu Arg Phe Asn Met Lys 
        355                 360                 365             


Asn Ala Lys Pro Val Ser Thr Pro Leu Ala Gly His Leu Lys Leu Ser 
    370                 375                 380                 


Lys Lys Met Cys Pro Thr Thr Val Glu Glu Lys Gly Asn Met Ala Lys 
385                 390                 395                 400 


Val Pro Tyr Ser Ser Ala Val Gly Ser Leu Met Tyr Ala Met Val Cys 
                405                 410                 415     


Thr Arg Pro Asp Ile Ala His Ala Val Gly Val Val Ser Arg Phe Leu 
            420                 425                 430         


Glu Asn Pro Gly Lys Glu His Trp Glu Ala Val Lys Trp Ile Leu Arg 
        435                 440                 445             


Tyr Leu Arg Gly Thr Thr Gly Asp Cys Leu Cys Phe Gly Gly Ser Asp 
    450                 455                 460                 


Pro Ile Leu Lys Gly Tyr Thr Asp Ala Asp Met Ala Gly Asp Ile Asp 
465                 470                 475                 480 


Asn Arg Lys Ser Ser Thr Gly Tyr Leu Phe Thr Phe Ser Gly Gly Ala 
                485                 490                 495     


Ile Ser Trp Gln Ser Lys Leu Gln Lys Cys Val Ala Leu Ser Thr Thr 
            500                 505                 510         


Glu Ala Glu Tyr Ile Ala Ala Thr Glu Thr Gly Lys Glu Met Ile Trp 
        515                 520                 525             


Leu Lys Arg Phe Leu Gln Glu Leu Gly Leu His Gln Lys Glu Tyr Val 
    530                 535                 540                 


Val Tyr Cys Asp Ser Gln Ser Ala Ile Asp Leu Ser Lys Asn Ser Met 
545                 550                 555                 560 


Tyr His Ala Arg Thr Lys His Ile Asp Val Arg Tyr His Trp Ile Arg 
                565                 570                 575     


Glu Met Val Asp Asp Glu Ser Leu Lys Val Leu Lys Ile Ser Thr Asn 
            580                 585                 590         


Glu Asn Pro Ala Asp Met Leu Thr Lys Val Val Pro Arg Asn Lys Phe 
        595                 600                 605             


Glu Leu Cys Lys Glu Leu Val Gly Met His Ser Asn 
    610                 615                 620 


<210>  8
<211>  603
<212>  PRT
<213>  Drosophila melanogaster

<400>  8

Ser Lys Lys Arg Lys Arg Asp Asp His Leu Asn Glu Ser Lys Gly Ser 
1               5                   10                  15      


Gly Asn Pro Asn Glu Ser Arg Glu Ser Glu Thr Ala Glu His Leu Lys 
            20                  25                  30          


Glu Ile Gly Ile Asp Asn Pro Thr Lys Asn Asp Gly Ile Glu Ile Ile 
        35                  40                  45              


Asn Arg Arg Ser Glu Arg Leu Lys Thr Lys Pro Gln Ile Ser Tyr Asn 
    50                  55                  60                  


Glu Glu Asp Asn Ser Leu Asn Lys Val Val Leu Asn Ala His Thr Ile 
65                  70                  75                  80  


Phe Asn Asp Val Pro Asn Ser Phe Asp Glu Ile Gln Tyr Arg Asp Asp 
                85                  90                  95      


Lys Ser Ser Trp Glu Glu Ala Ile Asn Thr Glu Leu Asn Ala His Lys 
            100                 105                 110         


Ile Asn Asn Thr Trp Thr Ile Thr Lys Arg Pro Glu Asn Lys Asn Ile 
        115                 120                 125             


Val Asp Ser Arg Trp Val Phe Ser Val Lys Tyr Asn Glu Leu Gly Asn 
    130                 135                 140                 


Pro Ile Arg Tyr Lys Ala Arg Leu Val Ala Arg Gly Phe Thr Gln Lys 
145                 150                 155                 160 


Tyr Gln Ile Asp Tyr Glu Glu Thr Phe Ala Pro Val Ala Arg Ile Ser 
                165                 170                 175     


Ser Phe Arg Phe Ile Leu Ser Leu Val Ile Gln Tyr Asn Leu Lys Val 
            180                 185                 190         


His Gln Met Asp Val Lys Thr Ala Phe Leu Asn Gly Thr Leu Lys Glu 
        195                 200                 205             


Glu Ile Tyr Met Arg Leu Pro Gln Gly Ile Ser Cys Asn Ser Asp Asn 
    210                 215                 220                 


Val Cys Lys Leu Asn Lys Ala Ile Tyr Gly Leu Lys Gln Ala Ala Arg 
225                 230                 235                 240 


Cys Trp Phe Glu Val Phe Glu Gln Ala Leu Lys Glu Cys Glu Phe Val 
                245                 250                 255     


Asn Ser Ser Val Asp Arg Cys Ile Tyr Ile Leu Asp Lys Gly Asn Ile 
            260                 265                 270         


Asn Glu Asn Ile Tyr Val Leu Leu Tyr Val Asp Asp Val Val Ile Ala 
        275                 280                 285             


Thr Gly Asp Met Thr Arg Met Asn Asn Phe Lys Arg Tyr Leu Met Glu 
    290                 295                 300                 


Lys Phe Arg Met Thr Asp Leu Asn Glu Ile Lys His Phe Ile Gly Ile 
305                 310                 315                 320 


Arg Ile Glu Met Gln Glu Asp Lys Ile Tyr Leu Ser Gln Ser Ala Tyr 
                325                 330                 335     


Val Lys Lys Ile Leu Ser Lys Phe Asn Met Glu Asn Cys Asn Ala Val 
            340                 345                 350         


Ser Thr Pro Leu Pro Ser Lys Ile Asn Tyr Glu Leu Leu Asn Ser Asp 
        355                 360                 365             


Glu Asp Cys Asn Thr Pro Cys Arg Ser Leu Ile Gly Cys Leu Met Tyr 
    370                 375                 380                 


Ile Met Leu Cys Thr Arg Pro Asp Leu Thr Thr Ala Val Asn Ile Leu 
385                 390                 395                 400 


Ser Arg Tyr Ser Ser Lys Asn Asn Ser Glu Leu Trp Gln Asn Leu Lys 
                405                 410                 415     


Arg Val Leu Arg Tyr Leu Lys Gly Thr Ile Asp Met Lys Leu Ile Phe 
            420                 425                 430         


Lys Lys Asn Leu Ala Phe Glu Asn Lys Ile Ile Gly Tyr Val Asp Ser 
        435                 440                 445             


Asp Trp Ala Gly Ser Glu Ile Asp Arg Lys Ser Thr Thr Gly Tyr Leu 
    450                 455                 460                 


Phe Lys Met Phe Asp Phe Asn Leu Ile Cys Trp Asn Thr Lys Arg Gln 
465                 470                 475                 480 


Asn Ser Val Ala Ala Ser Ser Thr Glu Ala Glu Tyr Met Ala Leu Phe 
                485                 490                 495     


Glu Ala Val Arg Glu Ala Leu Trp Leu Lys Phe Leu Leu Thr Ser Ile 
            500                 505                 510         


Asn Ile Lys Leu Glu Asn Pro Ile Lys Ile Tyr Glu Asp Asn Gln Gly 
        515                 520                 525             


Cys Ile Ser Ile Ala Asn Asn Pro Ser Cys His Lys Arg Ala Lys His 
    530                 535                 540                 


Ile Asp Ile Lys Tyr His Phe Ala Arg Glu Gln Val Gln Asn Asn Val 
545                 550                 555                 560 


Ile Cys Leu Glu Tyr Ile Pro Thr Glu Asn Gln Leu Ala Asp Ile Phe 
                565                 570                 575     


Thr Lys Pro Leu Pro Ala Ala Arg Phe Val Glu Leu Arg Asp Lys Leu 
            580                 585                 590         


Gly Leu Leu Gln Asp Asp Gln Ser Asn Ala Glu 
        595                 600             


<210>  9
<211>  677
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Moloney Murine Leukemia Virus RTmut_PE2

<400>  9

Thr Leu Asn Ile Glu Asp Glu Tyr Arg Leu His Glu Thr Ser Lys Glu 
1               5                   10                  15      


Pro Asp Val Ser Leu Gly Ser Thr Trp Leu Ser Asp Phe Pro Gln Ala 
            20                  25                  30          


Trp Ala Glu Thr Gly Gly Met Gly Leu Ala Val Arg Gln Ala Pro Leu 
        35                  40                  45              


Ile Ile Pro Leu Lys Ala Thr Ser Thr Pro Val Ser Ile Lys Gln Tyr 
    50                  55                  60                  


Pro Met Ser Gln Glu Ala Arg Leu Gly Ile Lys Pro His Ile Gln Arg 
65                  70                  75                  80  


Leu Leu Asp Gln Gly Ile Leu Val Pro Cys Gln Ser Pro Trp Asn Thr 
                85                  90                  95      


Pro Leu Leu Pro Val Lys Lys Pro Gly Thr Asn Asp Tyr Arg Pro Val 
            100                 105                 110         


Gln Asp Leu Arg Glu Val Asn Lys Arg Val Glu Asp Ile His Pro Thr 
        115                 120                 125             


Val Pro Asn Pro Tyr Asn Leu Leu Ser Gly Leu Pro Pro Ser His Gln 
    130                 135                 140                 


Trp Tyr Thr Val Leu Asp Leu Lys Asp Ala Phe Phe Cys Leu Arg Leu 
145                 150                 155                 160 


His Pro Thr Ser Gln Pro Leu Phe Ala Phe Glu Trp Arg Asp Pro Glu 
                165                 170                 175     


Met Gly Ile Ser Gly Gln Leu Thr Trp Thr Arg Leu Pro Gln Gly Phe 
            180                 185                 190         


Lys Asn Ser Pro Thr Leu Phe Asn Glu Ala Leu His Arg Asp Leu Ala 
        195                 200                 205             


Asp Phe Arg Ile Gln His Pro Asp Leu Ile Leu Leu Gln Tyr Val Asp 
    210                 215                 220                 


Asp Leu Leu Leu Ala Ala Thr Ser Glu Leu Asp Cys Gln Gln Gly Thr 
225                 230                 235                 240 


Arg Ala Leu Leu Gln Thr Leu Gly Asn Leu Gly Tyr Arg Ala Ser Ala 
                245                 250                 255     


Lys Lys Ala Gln Ile Cys Gln Lys Gln Val Lys Tyr Leu Gly Tyr Leu 
            260                 265                 270         


Leu Lys Glu Gly Gln Arg Trp Leu Thr Glu Ala Arg Lys Glu Thr Val 
        275                 280                 285             


Met Gly Gln Pro Thr Pro Lys Thr Pro Arg Gln Leu Arg Glu Phe Leu 
    290                 295                 300                 


Gly Lys Ala Gly Phe Cys Arg Leu Phe Ile Pro Gly Phe Ala Glu Met 
305                 310                 315                 320 


Ala Ala Pro Leu Tyr Pro Leu Thr Lys Pro Gly Thr Leu Phe Asn Trp 
                325                 330                 335     


Gly Pro Asp Gln Gln Lys Ala Tyr Gln Glu Ile Lys Gln Ala Leu Leu 
            340                 345                 350         


Thr Ala Pro Ala Leu Gly Leu Pro Asp Leu Thr Lys Pro Phe Glu Leu 
        355                 360                 365             


Phe Val Asp Glu Lys Gln Gly Tyr Ala Lys Gly Val Leu Thr Gln Lys 
    370                 375                 380                 


Leu Gly Pro Trp Arg Arg Pro Val Ala Tyr Leu Ser Lys Lys Leu Asp 
385                 390                 395                 400 


Pro Val Ala Ala Gly Trp Pro Pro Cys Leu Arg Met Val Ala Ala Ile 
                405                 410                 415     


Ala Val Leu Thr Lys Asp Ala Gly Lys Leu Thr Met Gly Gln Pro Leu 
            420                 425                 430         


Val Ile Leu Ala Pro His Ala Val Glu Ala Leu Val Lys Gln Pro Pro 
        435                 440                 445             


Asp Arg Trp Leu Ser Asn Ala Arg Met Thr His Tyr Gln Ala Leu Leu 
    450                 455                 460                 


Leu Asp Thr Asp Arg Val Gln Phe Gly Pro Val Val Ala Leu Asn Pro 
465                 470                 475                 480 


Ala Thr Leu Leu Pro Leu Pro Glu Glu Gly Leu Gln His Asn Cys Leu 
                485                 490                 495     


Asp Ile Leu Ala Glu Ala His Gly Thr Arg Pro Asp Leu Thr Asp Gln 
            500                 505                 510         


Pro Leu Pro Asp Ala Asp His Thr Trp Tyr Thr Asp Gly Ser Ser Leu 
        515                 520                 525             


Leu Gln Glu Gly Gln Arg Lys Ala Gly Ala Ala Val Thr Thr Glu Thr 
    530                 535                 540                 


Glu Val Ile Trp Ala Lys Ala Leu Pro Ala Gly Thr Ser Ala Gln Arg 
545                 550                 555                 560 


Ala Glu Leu Ile Ala Leu Thr Gln Ala Leu Lys Met Ala Glu Gly Lys 
                565                 570                 575     


Lys Leu Asn Val Tyr Thr Asp Ser Arg Tyr Ala Phe Ala Thr Ala His 
            580                 585                 590         


Ile His Gly Glu Ile Tyr Arg Arg Arg Gly Trp Leu Thr Ser Glu Gly 
        595                 600                 605             


Lys Glu Ile Lys Asn Lys Asp Glu Ile Leu Ala Leu Leu Lys Ala Leu 
    610                 615                 620                 


Phe Leu Pro Lys Arg Leu Ser Ile Ile His Cys Pro Gly His Gln Lys 
625                 630                 635                 640 


Gly His Ser Ala Glu Ala Arg Gly Asn Arg Met Ala Asp Gln Ala Ala 
                645                 650                 655     


Arg Lys Ala Ala Ile Thr Glu Thr Pro Asp Thr Ser Thr Leu Leu Ile 
            660                 665                 670         


Glu Asn Ser Ser Pro 
        675         


<210>  10
<211>  1954
<212>  PRT
<213>  Artificial

<220>
<223>  nCas9-CaMVRT

<400>  10

Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
            20                  25                  30          


Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys 
        35                  40                  45              


Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn 
    50                  55                  60                  


Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr 
65                  70                  75                  80  


Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg 
                85                  90                  95      


Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp 
            100                 105                 110         


Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp 
        115                 120                 125             


Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val 
    130                 135                 140                 


Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu 
145                 150                 155                 160 


Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
                165                 170                 175     


Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu 
            180                 185                 190         


Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln 
        195                 200                 205             


Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val 
    210                 215                 220                 


Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu 
225                 230                 235                 240 


Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe 
                245                 250                 255     


Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser 
            260                 265                 270         


Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr 
        275                 280                 285             


Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr 
    290                 295                 300                 


Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
305                 310                 315                 320 


Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser 
                325                 330                 335     


Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu 
            340                 345                 350         


Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile 
        355                 360                 365             


Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly 
    370                 375                 380                 


Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys 
385                 390                 395                 400 


Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu 
                405                 410                 415     


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr 
        435                 440                 445             


Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe 
465                 470                 475                 480 


Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe 
                485                 490                 495     


Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg 
            500                 505                 510         


Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys 
        515                 520                 525             


His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys 
    530                 535                 540                 


Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly 
545                 550                 555                 560 


Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys 
                565                 570                 575     


Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys 
            580                 585                 590         


Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser 
        595                 600                 605             


Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe 
    610                 615                 620                 


Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr 
625                 630                 635                 640 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr 
                645                 650                 655     


Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg 
            660                 665                 670         


Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile 
        675                 680                 685             


Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp 
    690                 695                 700                 


Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu 
705                 710                 715                 720 


Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp 
                725                 730                 735     


Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys 
            740                 745                 750         


Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val 
        755                 760                 765             


Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu 
    770                 775                 780                 


Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys 
785                 790                 795                 800 


Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu 
                805                 810                 815     


His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr 
            820                 825                 830         


Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile 
        835                 840                 845             


Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe 
    850                 855                 860                 


Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys 
865                 870                 875                 880 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys 
                885                 890                 895     


Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln 
            900                 905                 910         


Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu 
        915                 920                 925             


Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys 
945                 950                 955                 960 


Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu 
                965                 970                 975     


Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys 
            980                 985                 990         


Val Arg Glu Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1010                 1015                 1020             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1025                 1030                 1035             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1040                 1045                 1050             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1055                 1060                 1065             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1070                 1075                 1080             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1085                 1090                 1095             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1100                 1105                 1110             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1115                 1120                 1125             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1130                 1135                 1140             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1145                 1150                 1155             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1160                 1165                 1170             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1175                 1180                 1185             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1190                 1195                 1200             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1205                 1210                 1215             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1220                 1225                 1230             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1235                 1240                 1245             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1250                 1255                 1260             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1265                 1270                 1275             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1280                 1285                 1290             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1295                 1300                 1305             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1310                 1315                 1320             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1325                 1330                 1335             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1340                 1345                 1350             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1355                 1360                 1365             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1370                 1375                 1380             


Gly Gly  Asp Ser Gly Gly Ser  Ser Gly Gly Ser Ser  Gly Ser Glu 
    1385                 1390                 1395             


Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Gly 
    1400                 1405                 1410             


Ser Ser  Gly Gly Ser Ser Ser  Lys Thr Gln Gln Pro  Glu Pro Val 
    1415                 1420                 1425             


Asn Ile  Ser Thr Asn Lys Ile  Glu Asn Pro Leu Glu  Glu Ile Ala 
    1430                 1435                 1440             


Ile Leu  Ser Glu Gly Arg Arg  Leu Ser Glu Glu Lys  Leu Phe Ile 
    1445                 1450                 1455             


Thr Gln  Gln Arg Met Gln Lys  Thr Glu Glu Leu Leu  Glu Lys Val 
    1460                 1465                 1470             


Cys Ser  Glu Asn Pro Leu Asp  Pro Asn Lys Thr Lys  Gln Trp Met 
    1475                 1480                 1485             


Lys Ala  Ser Ile Lys Leu Ser  Asp Pro Ser Lys Ala  Ile Lys Val 
    1490                 1495                 1500             


Lys Pro  Met Lys Tyr Ser Pro  Met Asp Arg Glu Glu  Phe Asp Lys 
    1505                 1510                 1515             


Gln Ile  Lys Glu Leu Leu Asp  Leu Lys Val Ile Lys  Pro Ser Lys 
    1520                 1525                 1530             


Ser Pro  His Met Ala Pro Ala  Phe Leu Val Asn Asn  Glu Ala Glu 
    1535                 1540                 1545             


Asn Gly  Arg Gly Asn Lys Arg  Met Val Val Asn Tyr  Lys Ala Met 
    1550                 1555                 1560             


Asn Lys  Ala Thr Val Gly Asp  Ala Tyr Asn Leu Pro  Asn Lys Asp 
    1565                 1570                 1575             


Glu Leu  Leu Thr Leu Ile Arg  Gly Lys Lys Ile Phe  Ser Ser Phe 
    1580                 1585                 1590             


Asp Cys  Lys Ser Gly Phe Trp  Gln Val Leu Leu Asp  Gln Glu Ser 
    1595                 1600                 1605             


Arg Pro  Leu Thr Ala Phe Thr  Cys Pro Gln Gly His  Tyr Glu Trp 
    1610                 1615                 1620             


Asn Val  Val Pro Phe Gly Leu  Lys Gln Ala Pro Ser  Ile Phe Gln 
    1625                 1630                 1635             


Arg His  Met Asp Glu Ala Phe  Arg Val Phe Arg Lys  Phe Cys Cys 
    1640                 1645                 1650             


Val Tyr  Val Asp Asp Ile Val  Val Phe Ser Asn Asn  Glu Glu Asp 
    1655                 1660                 1665             


His Leu  Leu His Val Ala Met  Ile Leu Gln Lys Cys  Asn Gln His 
    1670                 1675                 1680             


Gly Ile  Ile Leu Ser Lys Lys  Lys Ala Gln Leu Phe  Lys Lys Lys 
    1685                 1690                 1695             


Ile Asn  Phe Leu Gly Leu Glu  Ile Asp Glu Gly Thr  His Lys Pro 
    1700                 1705                 1710             


Gln Gly  His Ile Leu Glu His  Ile Asn Lys Phe Pro  Asp Thr Leu 
    1715                 1720                 1725             


Glu Asp  Lys Lys Gln Leu Gln  Arg Phe Leu Gly Ile  Leu Thr Tyr 
    1730                 1735                 1740             


Ala Ser  Asp Tyr Ile Pro Asn  Leu Ala Gln Met Arg  Gln Pro Leu 
    1745                 1750                 1755             


Gln Ala  Lys Leu Lys Glu Asn  Val Pro Trp Lys Trp  Thr Lys Glu 
    1760                 1765                 1770             


Asp Thr  Leu Tyr Met Gln Lys  Val Lys Lys Asn Leu  Gln Gly Phe 
    1775                 1780                 1785             


Pro Pro  Leu His His Pro Leu  Pro Glu Glu Lys Leu  Ile Ile Glu 
    1790                 1795                 1800             


Thr Asp  Ala Ser Asp Asp Tyr  Trp Gly Gly Met Leu  Lys Ala Ile 
    1805                 1810                 1815             


Lys Ile  Asn Glu Gly Thr Asn  Thr Glu Leu Ile Cys  Arg Tyr Arg 
    1820                 1825                 1830             


Ser Gly  Ser Phe Lys Ala Ala  Glu Arg Asn Tyr His  Ser Asn Asp 
    1835                 1840                 1845             


Lys Glu  Thr Leu Ala Val Ile  Asn Thr Ile Lys Lys  Phe Ser Ile 
    1850                 1855                 1860             


Tyr Leu  Thr Pro Val His Phe  Leu Ile Arg Thr Asp  Asn Thr His 
    1865                 1870                 1875             


Phe Lys  Ser Phe Val Asn Leu  Asn Tyr Lys Gly Asp  Ser Lys Leu 
    1880                 1885                 1890             


Gly Arg  Asn Ile Arg Trp Gln  Ala Trp Leu Ser His  Tyr Ser Phe 
    1895                 1900                 1905             


Asp Val  Glu His Ile Lys Gly  Thr Asp Asn His Phe  Ala Asp Phe 
    1910                 1915                 1920             


Leu Ser  Arg Glu Phe Asn Lys  Val Asn Ser Ser Gly  Gly Ser Lys 
    1925                 1930                 1935             


Arg Thr  Ala Asp Gly Ser Glu  Phe Glu Pro Lys Lys  Lys Arg Lys 
    1940                 1945                 1950             


Val 
    


<210>  11
<211>  2040
<212>  PRT
<213>  Artificial

<220>
<223>  nCas9-LullabyRT

<400>  11

Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
            20                  25                  30          


Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys 
        35                  40                  45              


Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn 
    50                  55                  60                  


Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr 
65                  70                  75                  80  


Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg 
                85                  90                  95      


Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp 
            100                 105                 110         


Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp 
        115                 120                 125             


Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val 
    130                 135                 140                 


Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu 
145                 150                 155                 160 


Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
                165                 170                 175     


Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu 
            180                 185                 190         


Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln 
        195                 200                 205             


Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val 
    210                 215                 220                 


Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu 
225                 230                 235                 240 


Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe 
                245                 250                 255     


Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser 
            260                 265                 270         


Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr 
        275                 280                 285             


Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr 
    290                 295                 300                 


Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
305                 310                 315                 320 


Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser 
                325                 330                 335     


Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu 
            340                 345                 350         


Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile 
        355                 360                 365             


Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly 
    370                 375                 380                 


Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys 
385                 390                 395                 400 


Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu 
                405                 410                 415     


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr 
        435                 440                 445             


Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe 
465                 470                 475                 480 


Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe 
                485                 490                 495     


Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg 
            500                 505                 510         


Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys 
        515                 520                 525             


His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys 
    530                 535                 540                 


Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly 
545                 550                 555                 560 


Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys 
                565                 570                 575     


Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys 
            580                 585                 590         


Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser 
        595                 600                 605             


Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe 
    610                 615                 620                 


Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr 
625                 630                 635                 640 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr 
                645                 650                 655     


Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg 
            660                 665                 670         


Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile 
        675                 680                 685             


Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp 
    690                 695                 700                 


Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu 
705                 710                 715                 720 


Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp 
                725                 730                 735     


Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys 
            740                 745                 750         


Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val 
        755                 760                 765             


Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu 
    770                 775                 780                 


Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys 
785                 790                 795                 800 


Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu 
                805                 810                 815     


His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr 
            820                 825                 830         


Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile 
        835                 840                 845             


Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe 
    850                 855                 860                 


Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys 
865                 870                 875                 880 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys 
                885                 890                 895     


Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln 
            900                 905                 910         


Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu 
        915                 920                 925             


Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys 
945                 950                 955                 960 


Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu 
                965                 970                 975     


Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys 
            980                 985                 990         


Val Arg Glu Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1010                 1015                 1020             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1025                 1030                 1035             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1040                 1045                 1050             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1055                 1060                 1065             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1070                 1075                 1080             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1085                 1090                 1095             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1100                 1105                 1110             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1115                 1120                 1125             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1130                 1135                 1140             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1145                 1150                 1155             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1160                 1165                 1170             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1175                 1180                 1185             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1190                 1195                 1200             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1205                 1210                 1215             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1220                 1225                 1230             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1235                 1240                 1245             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1250                 1255                 1260             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1265                 1270                 1275             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1280                 1285                 1290             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1295                 1300                 1305             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1310                 1315                 1320             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1325                 1330                 1335             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1340                 1345                 1350             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1355                 1360                 1365             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1370                 1375                 1380             


Gly Gly  Asp Ser Gly Gly Ser  Ser Gly Gly Ser Ser  Gly Ser Glu 
    1385                 1390                 1395             


Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Gly 
    1400                 1405                 1410             


Ser Ser  Gly Gly Ser Ser Ser  Glu Met Ser Pro Phe  Pro Phe His 
    1415                 1420                 1425             


Tyr Ser  Arg Arg Pro Arg Thr  Pro His Glu Ser Gln  Pro Pro Ser 
    1430                 1435                 1440             


Cys Leu  Pro Ser Asp Val Pro  Thr Ser Thr Ile Asn  Ala Gln Ser 
    1445                 1450                 1455             


Asp Asp  Pro Pro Val Ala Pro  Leu Ile Asp Asp Pro  Leu Val Ser 
    1460                 1465                 1470             


Ser Pro  Gln Ser Ala Thr Pro  Arg Tyr Gly Leu Arg  Asp Arg Ser 
    1475                 1480                 1485             


Lys Ile  Met Pro His Pro Arg  Tyr Met Cys Ala Ala  Ile Asp Gly 
    1490                 1495                 1500             


Val Gln  Glu Pro Ser Thr Tyr  Gln Glu Ala Ala Ala  Ile Pro Glu 
    1505                 1510                 1515             


Trp Gln  Leu Ala Met Thr Glu  Glu Leu Ala Ala Leu  Glu Arg Thr 
    1520                 1525                 1530             


Arg Thr  Trp Asp Ile Val Pro  Leu Pro Cys His Ala  Ile Pro Ile 
    1535                 1540                 1545             


Thr Cys  Lys Trp Val Phe Lys  Val Lys Thr Arg Ser  Asp Gly Ser 
    1550                 1555                 1560             


Ile Glu  Arg Tyr Lys Ala Arg  Leu Val Ala Arg Gly  Phe Gln Gln 
    1565                 1570                 1575             


Glu His  Gly Arg Asp Tyr Asp  Glu Thr Phe Ala Pro  Val Ala His 
    1580                 1585                 1590             


Met Thr  Thr Val Arg Thr Leu  Val Ala Val Ala Ala  Ala Arg Ser 
    1595                 1600                 1605             


Trp Thr  Ile Ser Gln Met Asp  Val Lys Asn Ala Phe  Leu His Gly 
    1610                 1615                 1620             


Asp Leu  Lys Glu Glu Val Tyr  Met Lys Pro Pro Pro  Gly Val Asp 
    1625                 1630                 1635             


Ala Pro  Ala Asn Tyr Val Phe  Arg Leu Asn Arg Ala  Leu Tyr Gly 
    1640                 1645                 1650             


Leu Lys  Gln Ala Pro Arg Ala  Trp Phe Glu Arg Phe  Ser Ser Val 
    1655                 1660                 1665             


Val Cys  Ala Ala Gly Phe Ser  Pro Ser Asp His Asp  Pro Ala Leu 
    1670                 1675                 1680             


Phe Ile  His Val Ser Asp His  Gly Arg Thr Leu Leu  Leu Leu Tyr 
    1685                 1690                 1695             


Val Asp  Asp Met Leu Ile Thr  Gly Asp Asp Leu Glu  Tyr Ile Ala 
    1700                 1705                 1710             


Phe Val  Lys Gln Cys Leu Ser  Glu Gln Phe Met Met  Ser Asp Leu 
    1715                 1720                 1725             


Gly Pro  Leu Ser Tyr Phe Leu  Gly Ile Glu Val Thr  Asn Thr Glu 
    1730                 1735                 1740             


Asp Gly  Tyr Tyr Leu Ser Gln  His Arg Tyr Ile Asp  Asp Leu Ile 
    1745                 1750                 1755             


Thr Arg  Ser Gly Leu Thr Asp  Thr Lys Thr Ala Thr  Thr Pro Met 
    1760                 1765                 1770             


Glu Leu  His Val Gln Leu Arg  Ser Thr Glu Gly Val  Leu Leu Glu 
    1775                 1780                 1785             


Asp Pro  Ser Arg Tyr Arg Gln  Ile Val Gly Ser Leu  Val Tyr Leu 
    1790                 1795                 1800             


Thr Ile  Thr Arg Pro Asp Ile  Ala His Ile Val His  Val Leu Ser 
    1805                 1810                 1815             


Gln Phe  Val Ser Ala Pro Thr  Ser Val His Tyr Ser  His Leu Leu 
    1820                 1825                 1830             


Arg Val  Leu Arg Tyr Leu Arg  Gly Thr Ala Ser Arg  Gln Leu Phe 
    1835                 1840                 1845             


Tyr Ala  Arg Ser Ser Gln Leu  His Leu His Ala Tyr  Ser Asp Ser 
    1850                 1855                 1860             


Thr Trp  Ala Ser Asp Pro Met  Asp Arg Arg Ser Ile  Thr Gly Tyr 
    1865                 1870                 1875             


Cys Ile  Phe Leu Gly Thr Ser  Leu Ile Ala Trp Lys  Ser Lys Lys 
    1880                 1885                 1890             


Gln Thr  Ala Val Ser Arg Ser  Ser Thr Glu Ser Glu  Leu Arg Ala 
    1895                 1900                 1905             


Leu Ala  Thr Thr Thr Ser Glu  Ile Val Trp Leu Arg  Trp Leu Leu 
    1910                 1915                 1920             


Ser Asp  Leu Gly Val Glu Cys  Lys Asp Pro Thr Pro  Leu Leu Cys 
    1925                 1930                 1935             


Asp Asn  Thr Gly Ala Ile Gln  Ile Ala Asn Asp Pro  Val Lys His 
    1940                 1945                 1950             


Glu Leu  Thr Lys His Ile Gly  Val Asp Ala Ser Phe  Thr Arg Ser 
    1955                 1960                 1965             


His Cys  Gln Gln Ser Thr Ile  Ser Leu Arg Tyr Val  Pro Ser Glu 
    1970                 1975                 1980             


Leu Gln  Val Ala Asp Phe Phe  Thr Lys Ala Gln Thr  Arg Glu Gln 
    1985                 1990                 1995             


His Arg  Leu Tyr Ile Leu Lys  Leu Asn Val Ile Asp  Pro Pro Asn 
    2000                 2005                 2010             


His Ser  Pro Asn Pro Pro Ser  Gly Gly Ser Lys Arg  Thr Ala Asp 
    2015                 2020                 2025             


Gly Ser  Glu Phe Glu Pro Lys  Lys Lys Arg Lys Val  
    2030                 2035                 2040 


<210>  12
<211>  2060
<212>  PRT
<213>  Artificial

<220>
<223>  nCas9-TnT1RT

<400>  12

Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
            20                  25                  30          


Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys 
        35                  40                  45              


Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn 
    50                  55                  60                  


Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr 
65                  70                  75                  80  


Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg 
                85                  90                  95      


Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp 
            100                 105                 110         


Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp 
        115                 120                 125             


Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val 
    130                 135                 140                 


Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu 
145                 150                 155                 160 


Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
                165                 170                 175     


Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu 
            180                 185                 190         


Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln 
        195                 200                 205             


Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val 
    210                 215                 220                 


Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu 
225                 230                 235                 240 


Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe 
                245                 250                 255     


Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser 
            260                 265                 270         


Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr 
        275                 280                 285             


Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr 
    290                 295                 300                 


Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
305                 310                 315                 320 


Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser 
                325                 330                 335     


Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu 
            340                 345                 350         


Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile 
        355                 360                 365             


Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly 
    370                 375                 380                 


Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys 
385                 390                 395                 400 


Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu 
                405                 410                 415     


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr 
        435                 440                 445             


Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe 
465                 470                 475                 480 


Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe 
                485                 490                 495     


Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg 
            500                 505                 510         


Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys 
        515                 520                 525             


His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys 
    530                 535                 540                 


Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly 
545                 550                 555                 560 


Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys 
                565                 570                 575     


Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys 
            580                 585                 590         


Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser 
        595                 600                 605             


Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe 
    610                 615                 620                 


Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr 
625                 630                 635                 640 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr 
                645                 650                 655     


Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg 
            660                 665                 670         


Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile 
        675                 680                 685             


Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp 
    690                 695                 700                 


Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu 
705                 710                 715                 720 


Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp 
                725                 730                 735     


Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys 
            740                 745                 750         


Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val 
        755                 760                 765             


Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu 
    770                 775                 780                 


Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys 
785                 790                 795                 800 


Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu 
                805                 810                 815     


His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr 
            820                 825                 830         


Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile 
        835                 840                 845             


Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe 
    850                 855                 860                 


Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys 
865                 870                 875                 880 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys 
                885                 890                 895     


Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln 
            900                 905                 910         


Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu 
        915                 920                 925             


Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys 
945                 950                 955                 960 


Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu 
                965                 970                 975     


Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys 
            980                 985                 990         


Val Arg Glu Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1010                 1015                 1020             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1025                 1030                 1035             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1040                 1045                 1050             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1055                 1060                 1065             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1070                 1075                 1080             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1085                 1090                 1095             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1100                 1105                 1110             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1115                 1120                 1125             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1130                 1135                 1140             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1145                 1150                 1155             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1160                 1165                 1170             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1175                 1180                 1185             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1190                 1195                 1200             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1205                 1210                 1215             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1220                 1225                 1230             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1235                 1240                 1245             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1250                 1255                 1260             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1265                 1270                 1275             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1280                 1285                 1290             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1295                 1300                 1305             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1310                 1315                 1320             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1325                 1330                 1335             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1340                 1345                 1350             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1355                 1360                 1365             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1370                 1375                 1380             


Gly Gly  Asp Ser Gly Gly Ser  Ser Gly Gly Ser Ser  Gly Ser Glu 
    1385                 1390                 1395             


Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Gly 
    1400                 1405                 1410             


Ser Ser  Gly Gly Ser Ser Val  Arg Thr Ala Ala Asp  Met Ser Glu 
    1415                 1420                 1425             


Lys Val  Lys Asn Gly Ile Ile  Pro Asn Phe Val Thr  Ile Pro Ser 
    1430                 1435                 1440             


Thr Ser  Asn Asn Pro Thr Ser  Ala Glu Ser Thr Thr  Asp Glu Val 
    1445                 1450                 1455             


Ser Glu  Gln Gly Glu Gln Pro  Gly Glu Val Ile Glu  Gln Gly Glu 
    1460                 1465                 1470             


Gln Leu  Asp Glu Gly Val Glu  Glu Val Glu His Pro  Thr Gln Gly 
    1475                 1480                 1485             


Glu Glu  Gln His Gln Pro Leu  Arg Arg Ser Glu Arg  Pro Arg Val 
    1490                 1495                 1500             


Glu Ser  Arg Arg Tyr Pro Ser  Thr Glu Tyr Val Leu  Ile Ser Asp 
    1505                 1510                 1515             


Asp Arg  Glu Pro Glu Ser Leu  Lys Glu Val Leu Ser  His Pro Glu 
    1520                 1525                 1530             


Lys Asn  Gln Leu Met Lys Ala  Met Gln Glu Glu Met  Glu Ser Leu 
    1535                 1540                 1545             


Gln Lys  Asn Gly Thr Tyr Lys  Leu Val Glu Leu Pro  Lys Gly Lys 
    1550                 1555                 1560             


Arg Pro  Leu Lys Cys Lys Trp  Val Phe Lys Leu Lys  Lys Asp Gly 
    1565                 1570                 1575             


Asp Cys  Lys Leu Val Arg Tyr  Lys Ala Arg Leu Val  Val Lys Gly 
    1580                 1585                 1590             


Phe Glu  Gln Lys Lys Gly Ile  Asp Phe Asp Glu Ile  Phe Ser Pro 
    1595                 1600                 1605             


Val Val  Lys Met Thr Ser Ile  Arg Thr Ile Leu Ser  Leu Ala Ala 
    1610                 1615                 1620             


Ser Leu  Asp Leu Glu Val Glu  Gln Leu Asp Val Lys  Thr Ala Phe 
    1625                 1630                 1635             


Leu His  Gly Asp Leu Glu Glu  Glu Ile Tyr Met Glu  Gln Pro Glu 
    1640                 1645                 1650             


Gly Phe  Glu Val Ala Gly Lys  Lys His Met Val Cys  Lys Leu Asn 
    1655                 1660                 1665             


Lys Ser  Leu Tyr Gly Leu Lys  Gln Ala Pro Arg Gln  Trp Tyr Met 
    1670                 1675                 1680             


Lys Phe  Asp Ser Phe Met Lys  Ser Gln Thr Tyr Leu  Lys Thr Tyr 
    1685                 1690                 1695             


Ser Asp  Pro Cys Val Tyr Phe  Lys Arg Phe Ser Glu  Asn Asn Phe 
    1700                 1705                 1710             


Ile Ile  Leu Leu Leu Tyr Val  Asp Asp Met Leu Ile  Val Gly Lys 
    1715                 1720                 1725             


Asp Lys  Gly Leu Ile Ala Lys  Leu Lys Gly Asp Leu  Ser Lys Ser 
    1730                 1735                 1740             


Phe Asp  Met Lys Asp Leu Gly  Pro Ala Gln Gln Ile  Leu Gly Met 
    1745                 1750                 1755             


Lys Ile  Val Arg Glu Arg Thr  Ser Arg Lys Leu Trp  Leu Ser Gln 
    1760                 1765                 1770             


Glu Lys  Tyr Ile Glu Arg Val  Leu Glu Arg Phe Asn  Met Lys Asn 
    1775                 1780                 1785             


Ala Lys  Pro Val Ser Thr Pro  Leu Ala Gly His Leu  Lys Leu Ser 
    1790                 1795                 1800             


Lys Lys  Met Cys Pro Thr Thr  Val Glu Glu Lys Gly  Asn Met Ala 
    1805                 1810                 1815             


Lys Val  Pro Tyr Ser Ser Ala  Val Gly Ser Leu Met  Tyr Ala Met 
    1820                 1825                 1830             


Val Cys  Thr Arg Pro Asp Ile  Ala His Ala Val Gly  Val Val Ser 
    1835                 1840                 1845             


Arg Phe  Leu Glu Asn Pro Gly  Lys Glu His Trp Glu  Ala Val Lys 
    1850                 1855                 1860             


Trp Ile  Leu Arg Tyr Leu Arg  Gly Thr Thr Gly Asp  Cys Leu Cys 
    1865                 1870                 1875             


Phe Gly  Gly Ser Asp Pro Ile  Leu Lys Gly Tyr Thr  Asp Ala Asp 
    1880                 1885                 1890             


Met Ala  Gly Asp Ile Asp Asn  Arg Lys Ser Ser Thr  Gly Tyr Leu 
    1895                 1900                 1905             


Phe Thr  Phe Ser Gly Gly Ala  Ile Ser Trp Gln Ser  Lys Leu Gln 
    1910                 1915                 1920             


Lys Cys  Val Ala Leu Ser Thr  Thr Glu Ala Glu Tyr  Ile Ala Ala 
    1925                 1930                 1935             


Thr Glu  Thr Gly Lys Glu Met  Ile Trp Leu Lys Arg  Phe Leu Gln 
    1940                 1945                 1950             


Glu Leu  Gly Leu His Gln Lys  Glu Tyr Val Val Tyr  Cys Asp Ser 
    1955                 1960                 1965             


Gln Ser  Ala Ile Asp Leu Ser  Lys Asn Ser Met Tyr  His Ala Arg 
    1970                 1975                 1980             


Thr Lys  His Ile Asp Val Arg  Tyr His Trp Ile Arg  Glu Met Val 
    1985                 1990                 1995             


Asp Asp  Glu Ser Leu Lys Val  Leu Lys Ile Ser Thr  Asn Glu Asn 
    2000                 2005                 2010             


Pro Ala  Asp Met Leu Thr Lys  Val Val Pro Arg Asn  Lys Phe Glu 
    2015                 2020                 2025             


Leu Cys  Lys Glu Leu Val Gly  Met His Ser Asn Ser  Gly Gly Ser 
    2030                 2035                 2040             


Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  Lys Lys Arg 
    2045                 2050                 2055             


Lys Val  
    2060 


<210>  13
<211>  1917
<212>  DNA
<213>  Zea mays (var. A188)

<400>  13
atggccaccg ccgccaccgc ggccgccgcg ctcaccggcg ccactaccgc tacgcccaag       60

tcgaggcgcc gagcccacca cttggccacc cggcgcgccc tcgccgcgcc catcaggtgc      120

tcagcgttgt cacgcgccac gccgacggct cccccggcca ctccgctacg tccgtggggc      180

cccaacgagc cccgcaaggg ctccgacatc ctcgtcgagg ctctcgagcg ctgtggcgtc      240

cgtgacgtct tcgcctaccc cggcggcgca tccatggaga tccaccaggc actcacccgc      300

tcccccgtca tcgccaacca cctcttccgc cacgaacaag gggaggcctt cgccgcctcc      360

ggctacgcgc gctcctcggg ccgcgttggc gtctgcatcg ccacctccgg ccccggcgcc      420

accaacctag tctctgcgct cgcagacgcg ttgctcgact ccgtccccat tgtcgccatc      480

acgggacagg tgccgcgacg catgattggc accgacgcct ttcaggagac gcccatcgtc      540

gaggtcaccc gctccatcac caagcacaac tacctggtcc tcgacgtcga cgacatcccc      600

cgcgtcgtgc aggaggcctt cttcctcgca tcctctggtc gcccggggcc ggtgcttgtt      660

gacatcccca aggacatcca gcagcagatg gcggtgccgg cctgggacac gcccatgagt      720

ctgcctgggt acatcgcgcg ccttcccaag cctcccgcga ctgaatttct tgagcaggtg      780

ctgcgtcttg ttggtgaatc acggcgccct gttctttatg ttggcggtgg ctgtgcagta      840

tcaggtgagg agttgtgccg ctttgtggag ttgactggaa tcccagtcac aactactctt      900

atgggccttg gcaacttccc cagcgacgac ccactgtcac tgcgcatgct tggtatgcat      960

ggcacagtgt atgcaaatta tgcagtggat aaggccgatc tgttgcttgc atttggtgtg     1020

cggtttgatg atcgtgtgac agggaaaatt gaggcttttg caggcagagc taagattgtg     1080

cacattgata ttgatcctgc tgagattggc aagaacaagc agccacatgt gtccatctgt     1140

gcagatgtta agcttgcttt gcagggcatg aatactcttc tggaaggaag cacatcaaag     1200

aagagctttg acttcggctc atggcatgat gaattggatc agcaaaagag ggagtttccc     1260

cttgggtata aaatcttcaa tgaggaaatc cagccacaat atgctattca ggttcttgat     1320

gagttgacga aggggaaggc catcattgcc acaggtgttg ggcagcacca gatgtgggcg     1380

gcacagtatt acacttacaa gcggccaagg cagtggctgt cttcagctgg tcttggggct     1440

atgggatttg gtttgccggc tgctgctggt gctgctgtgg ccaacccagg tgtcactgtt     1500

gttgacatcg acggagatgg tagcttcctc atgaacattc aggagctagc tatgatccgt     1560

attgagaacc tcccagtcaa ggtctttgtg ctaaacaacc agcacctcgg gatggtggtg     1620

cagtgggagg acaggttcta taaggccaat agagcacaca cattcttggg aaacccagag     1680

aacgaaagtg agatatatcc agattttgtg gcaattgcca aagggttcaa cattccagca     1740

gtccgtgtga caaagaagag cgaagtccat gcagcaatca agaagatgct tgaggctcca     1800

gggccgtacc tcttggatat aatcgtcccg caccaggagc atgtgttgcc tatgatccct     1860

agtggtgggg ctttcaagga tatgatcctg gatggtgatg gcaggactgt gtattga        1917


<210>  14
<211>  1917
<212>  DNA
<213>  Zea mays (var. A188)

<400>  14
atggccaccg ccgccgccgc gtctaccgcg ctcactggcg ccactaccgc tgcgcccaag       60

gcgaggcgcc gggcgcacct cctggccacc cgccgcgccc tcgccgcgcc catcaggtgc      120

tcagcggcgt cacccgccat gccgatggct cccccggcca ccccgctccg gccgtggggc      180

cccaccgagc cccgcaaggg tgctgacatc ctcgtcgagt ccctcgagcg ctgcggcgtc      240

cgcgacgtct tcgcctaccc cggcggcgcg tccatggaga tccaccaggc actcacccgc      300

tcccccgtca tcgccaacca cctcttccgc cacgagcaag gggaggcctt tgccgcctcc      360

ggctacgcgc gctcctcggg ccgcgtcggc gtctgcatcg ccacctccgg ccccggcgcc      420

accaacctag tctccgcgct cgccgacgcg ctgctcgatt ccgtccccat ggtcgccatc      480

acgggacagg tgccgcgacg catgattggc accgacgcct tccaggagac gcccatcgtc      540

gaggtcaccc gctccatcac caagcacaac tacctggtcc tcgacgtcga cgacatcccc      600

cgcgtcgtgc aggaggcttt cttcctcgcc tcctctggtc gaccagggcc ggtgcttgtc      660

gacatcccca aggacatcca gcagcagatg gcggtgcctg tctgggacaa gcccatgagt      720

ctgcctgggt acattgcgcg ccttcccaag ccccctgcga ctgagttgct tgagcaggtg      780

ctgcgtcttg ttggtgaatc gcggcgccct gttctttatg tgggcggtgg ctgcgcagca      840

tctggtgagg agttgcgacg ctttgtggag ctgactggaa tcccggtcac aactactctt      900

atgggcctcg gcaacttccc cagcgacgac ccactgtctc tgcgcatgct aggtatgcat      960

gggacggtgt atgcaaatta tgcagtggat aaggccgatc tgttgcttgc acttggtgtg     1020

cggtttgatg atcgcgtgac agggaagatt gaggcttttg caagcagggc taagattgtg     1080

cacgttgata ttgatccggc tgagattggc aagaacaagc agccacatgt gtccatctgt     1140

gcagatgtta agcttgcttt gcagggcatg aatgctcttc ttgaaggaag cacatcaaag     1200

aagagctttg actttggctc atggaacgat gagttggatc agcagaagag ggaattcccc     1260

cttgggtata aaacatctaa tgaggagatc cagccacaat atgctattca ggttcttgat     1320

gagctgacga aaggcgaggc catcatcggc acaggtgttg ggcagcacca gatgtgggcg     1380

gcacagtact acacttacaa gcggccaagg cagtggttgt cttcagctgg tcttggggct     1440

atgggatttg gtttgccggc tgctgctggt gcttctgtgg caaacccagg tgtcactgtt     1500

gttgacatcg atggagatgg tagctttctc atgaacgttc aggagctagc tatgatccga     1560

attgagaacc tcccagtgaa ggtctttgtg ctaaacaacc agcacctggg gatggtggtg     1620

cagtgggagg acaggttcta taaggccaac agagcgcaca catacttggg aaacccagag     1680

aatgaaagtg agatatatcc agatttcgtg acgatcgcca aagggttcaa cattccagcg     1740

gtccgtgtga caaagaagaa cgaagtccgc gcagcgataa agaagatgct cgagactcca     1800

gggccgtacc tcttggatat aatcgtccca caccaggagc atgtgttgcc tatgatccct     1860

agtggtgggg ctttcaagga tatgatcctg gatggtgatg gcaggactgt gtactga        1917


<210>  15
<211>  572
<212>  DNA
<213>  Zea mays

<400>  15
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt tt                                    572


<210>  16
<211>  704
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_pegRNA_488forw

<400>  16
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgcatggtc gccatcacgg gacgttttag      600

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      660

agtcggtgcg tcgcgacacc tgtcccgtga tggcgacttt tttt                       704


<210>  17
<211>  702
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_pegRNA_493rev

<400>  17
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgtcggtgc caatcatgcg tcggttttag      600

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      660

agtcggtgca caggtgtcgc gacgcatgat tggcattttt tt                         702


<210>  18
<211>  702
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_pegRNA_1858rev

<400>  18
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgtccttga aagccccacc actgttttag      600

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      660

agtcggtgct atgatcccta atggtggggc tttcattttt tt                         702


<210>  19
<211>  700
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_pegRNA_1863forw

<400>  19
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgtgttgcc tatgatccct aggttttaga      600

gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga      660

gtcggtgcgc cccaccatta gggatcatag gcattttttt                            700


<210>  20
<211>  675
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_gRNA_443rev

<400>  20
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgacggaat cgagcagcgc gtgttttaga      600

gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga      660

gtcggtgctt ttttt                                                       675


<210>  21
<211>  676
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_gRNA_542forw

<400>  21
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgccaggag acgcccatcg tcggttttag      600

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      660

agtcggtgct tttttt                                                      676


<210>  22
<211>  676
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_gRNA_1800forw

<400>  22
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgaagaaga tgctcgagac tccgttttag      600

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      660

agtcggtgct tttttt                                                      676


<210>  23
<211>  675
<212>  DNA
<213>  Artificial

<220>
<223>  ZmU6_gRNA_1809rev

<400>  23
ccttcttttg tttcacttat cagttttcac caagattgca tgccacttgg cgggttcatc       60

acgaagaacc ttccaataat gcaaaactgt ggacggacct tttttttaga ataatcgttc      120

taacaaaatt gcatagccct gtctgtctct tagttgatca tcagattggc cagtagcata      180

aactgaggta gccttggtcc atgcggcaaa aaaaaaagaa ccatctctct ctcttttgcc      240

agccaggtac actattgtgt tttacctctt ttttagtcta aaaaatactt gtcatttatt      300

ttctatccag ctgacaatct ttatggcccc tgacgaaata gttcgtggca aactggcaat      360

ggtcttttgc aaaacacgac gctgtgcacg tcagcacgtg agcttgcgat gtccactagg      420

gagctccatc cactgatcca cccccacgcg gcgtggcgtc gtcattaacg gcttgtgggg      480

aagggaacga gcaactaacc gataattagt accagaccgg ccagtgaacg atgccaaaac      540

cggcttataa gctcagctgc gacaaccgtt ttgtgggacg attatatcca aggttttaga      600

gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga      660

gtcggtgctt ttttt                                                       675


<210>  24
<211>  1992
<212>  DNA
<213>  Zea mays

<400>  24
gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta       60

taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt      120

atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca      180

gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt      240

ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg      300

caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta      360

gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct      420

ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa      480

tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta      540

aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt      600

ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca      660

cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg      720

ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag      780

gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc ccaccgctcc      840

ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac cctctttccc      900

caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa atccacccgt      960

cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct ctaccttctc     1020

tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg     1080

ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta     1140

cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg     1200

ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt     1260

ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt     1320

ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat     1380

cggagtagaa ttaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat     1440

gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta     1500

ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc     1560

gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag     1620

aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata     1680

catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg     1740

ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct     1800

ctaaccttga gtacctatct attataataa acaagtatgt tttataatta ttttgatctt     1860

gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc     1920

atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg     1980

ttacttctgc ag                                                         1992


<210>  25
<211>  250
<212>  DNA
<213>  Agrobacterium tumefaciens

<400>  25
gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg       60

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc      120

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac      180

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct      240

atgttactag                                                             250


<210>  26
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  ZmALS_165_for

<400>  26
ccgtcatcgc caaccacctc tt                                                22


<210>  27
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  ZmALS_165_rev

<400>  27
ttggtgatgg agcgggtgac                                                   20


<210>  28
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  ZmALS_621_for

<400>  28
gaaagtgaga tatatccaga tttcg                                             25


<210>  29
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  ZmALS_621_rev

<400>  29
tcagtacaca gtcctgccat cacc                                              24


<210>  30
<211>  2145
<212>  DNA
<213>  Triticum aestivum (var. Fielder)

<400>  30
gcatttgaaa ctgcagtgca gaagtcatgg tctaacattt ctagtgacaa taaccgatgt       60

tatgttaaag caacggagct ggtgtttgct caaaagaatg ggtcatgggg cactcctgta      120

attcctatgg agcgtcctgc tgggctcaat gacattggta tggtagcttg gatcttggac      180

atgtccactc ctgaatatcc caatggcagg cagattgttg tcatcgcaaa tgatattact      240

tttagagctg gatcgtttgg tccaagggaa gatgcatttt ttgaaactgt taccaaccta      300

gcttgtgaga ggaagcttcc tctcatctac ttggcagcaa actctggtgc tcggatcggc      360

atagcagatg aagtaaaatc gtgcttccgt gttggatggt ctgatgatgg cagccctgaa      420

cgtgggtttc agtacattta tctgactgaa gaagaccatg ctcgtattag cacttctgtt      480

atagcgcaca agatgcagct tgataatggt gaaattaggt gggttattga ttctgttgtg      540

gggaaggagg atgggctagg tgtggagaac atacatggaa gtgctgctat tgccagtgcc      600

tattctaggg cctatgagga gacatttacg cttacatttg tgactggacg gactgttgga      660

ataggagcat atcttgctcg acttggcata cggtgcatac agcgtactga ccagcccatt      720

atcctaaccg ggttctctgc tttgaacaag cttcttggcc gggaagtgta cagctcccac      780

atgcagttgg gtggccccaa aattatggcg acaaacggtg ttgtccatct gacagtttca      840

gatgaccttg aaggtgtgtc taatatattg aggtggctca gctatgttcc tgccaacatt      900

ggtggacctc ttcctattac aaaatctttg gacccacctg acagacccgt tgcatatatc      960

cctgagaata catgtgatcc tcgtgcagcc atcagtggca ttgatgatag ccaagggaaa     1020

tggttggggg gcatgttcga caaagacagt tttgtggaga catttgaagg atgggcgaag     1080

tcagtagtta ctggcagagc gaaactcgga gggattccgg tgggtgttat agctgtggag     1140

acacagacta tgatgcagct catccctgct gatccaggcc agcttgattc ccatgagcgg     1200

tctgttcctc gtgctgggca agtctggttt ccagattcag ttactaagac agctcaagca     1260

atgctggact tcaaccgtga aggattacct ctgttcatcc ttgctaactg gagaggcttc     1320

tctggtgggc aaagagatct ttttgaagga atccttcagg ctgggtcaac aattgttgag     1380

aaccttagga catacaatca gcctgccttt gtatatatcc ccaaggctgc agagctacgt     1440

ggaggggctt gggtcgtgat tgatagcaag ataaatccag atcgaattga gttctatgct     1500

gagaggactg caaagggtaa tgttcttgaa cctcaagggt tgattgagat caagttcagg     1560

tcagaggaac tccaagagtg catgggcagg cttgacccag aattgataaa tctgaaggca     1620

aaactcctgg gagcaaagca tgaaaatgga agtctatctg agtcagaatc ccttcagaag     1680

agcatagaag cccggaagaa acagttgttg cctttgtata ctcaaattgc ggtgcggttt     1740

gctgaattgc atgacacttc ccttagaatg gctgctaagg gtgtgattaa gaaggttgta     1800

gactgggaag attctaggtc tttcttctac aagagattac ggaggaggat atccgaggac     1860

gttcttgcaa aggaaattag aggtgtaagt ggcaagcagt tctctcacca atcagcaatc     1920

gagctgatcc agaaatggta cttggcttct aagggagctg aagcagcaag cactgaatgg     1980

gatgatgacg atgcttttgt tgcctggagg gaaaaccctg aaaactacca ggagtatatc     2040

aaagaactta gggctcaaag ggtatctcag ttgctctcag atgttgcaga ctccagtcca     2100

gatctagaag ccttgccaca gggtctttct atgctactag agaag                     2145


<210>  31
<211>  2145
<212>  DNA
<213>  Triticum aestivum (var. Fielder)

<400>  31
gcatttgaaa ctgcagtgca gaagtcatgg tctaacattt ctagtgacaa taaccgatgt       60

tatgttaaag caacggagct ggtgtttgct cacaagaatg ggtcatgggg cactcctgta      120

attcctatgg agcgtcctgc tgggctcaat gacattggta tggtagcttg gatcttggac      180

atgtccactc ctgaatatcc caatggcagg cagattgttg tcatcgcaaa tgatattact      240

tttagagctg gatcgtttgg tccaagggaa gatgcatttt ttgaaactgt taccaaccta      300

gcttgtgaga ggaagcttcc tctcatctac ttggcagcaa actctggtgc tcggatcggc      360

atagcagatg aagtaaaatc ttgcttccgt gttggatggt ctgatgatgg cagccctgaa      420

cgtgggtttc agtacattta tctgactgaa gaagaccatg ctcgtattag cacttctgtt      480

atagcgcaca agatgcagct tgataatggt gaaattaggt gggttatcga ttctgttgtg      540

gggaaggagg atgggctagg tgtggagaac atacatggaa gtgctgctat tgccagtgcc      600

tattctaggg cctatgagga gacatttacg cttacatttg tgactggacg gactgttgga      660

ataggagcat atcttgctcg acttggcata cggtgcatac agcgtactga ccagcccatt      720

atcctaactg ggttctctgc cttgaacaag cttcttggcc gggaagttta cagctcccac      780

atgcagttgg gtggccccaa aattatggcg acaaacggtg ttgtccatct gacagtttca      840

gatgaccttg aaggtgtatc taatatattg aggtggctca gctatgttcc tgccaacatt      900

ggtggacctc ttcctattac aaaatctttg gacccacctg acagacccgt tgcttacatc      960

cctgagaata catgcgatcc tcgtgctgcc atcagtggca ttgatgatag ccaagggaaa     1020

tggttggggg gcatgttcga caaagacagt tttgtggaga catttgaagg atgggcgaag     1080

tcagttgtta ctggcagagc taaactcgga gggattccgg tgggtgttat agctgtggag     1140

acacagacta tgatgcagct catccctgct gatccaggcc agcttgattc ccatgagcga     1200

tctgttcctc gtgctgggca agtctggttt ccagattcag ctactaagac agcgcaggca     1260

atgctggact tcaaccgtga aggattacct ctgttcatcc ttgctaactg gagaggcttc     1320

tctggtggac aaagagatct ttttgaagga atccttcagg ctgggtcaac aattgttgag     1380

aaccttagga catacaatca gcctgccttt gtatatatcc ccaaggctgc agagctacgt     1440

ggaggggctt gggtcgtgat tgatagcaag ataaatccag atcgcattga gttctatgct     1500

gagaggactg caaagggcaa tgttctcgaa cctcaagggt tgatcgagat caagttcagg     1560

tcagaggaac tccaagagtg catgggtagg cttgatccag aattgataaa tctgaaggca     1620

aagctccagg gagtaaagca tgaaaatgga agtctacctg agtcagaatc ccttcagaag     1680

agcatagaag cccggaagaa acagttgttg cctttgtata ctcaaattgc ggtacggttc     1740

gctgaattgc atgacacttc ccttagaatg gctgctaagg gtgtgattaa gaaggttgta     1800

gactgggaag attctaggtc gttcttctac aagagattac ggaggaggat atccgaggat     1860

gttcttgcga aggaaattag aggtgtaagt ggcaagcagt tttctcacca atcggcaatc     1920

gagctgatcc agaaatggta cttggcctct aagggagctg aaacaggaag cactgaatgg     1980

gatgatgacg atgcttttgt tgcctggagg gaaaaccctg aaaactacca ggagtatatc     2040

aaagaactca gggctcaaag ggtatctcag ttgctctcag atgttgcaga ctccagtcca     2100

gatctagaag ccttgccaca gggtctttct atgctactag agaag                     2145


<210>  32
<211>  2145
<212>  DNA
<213>  Triticum aestivum (var. Fielder)

<400>  32
gcatttgaaa ctgcagtgca gaagtcatgg tctaacattt ctagtgacac taaccgatgt       60

tatgttaaag cgacggagct ggtgtttgct cacaagaacg ggtcatgggg cactcctgta      120

attcctatgg agcgtcctgc tgggctcaat gacattggta tggtagcttg gatcttggac      180

atgtccactc ctgaatatcc caatggcagg cagattgttg tcatcgcaaa tgatattact      240

tttagagctg gatcgtttgg tccaagggaa gatgcatttt ttgaaactgt taccaaccta      300

gcttgtgaga ggaagcttcc tctcatctac ttggcagcaa actctggtgc tcggatcggc      360

atagcagatg aagtaaaatc ttgcttccgt gttggatggt ctgatgatgg cagccctgaa      420

cgtgggtttc aatatattta tctgactgaa gaagaccatg ctcgtattag cgcttctgtt      480

atagcgcaca agatgcagct tgataatggt gaaattaggt gggttattga ttctgttgta      540

gggaaggagg atgggctagg tgtggagaac atacatggaa gtgctgctat tgccagtgcc      600

tattctaggg cctatgagga gacatttacg cttacatttg tgactggaag gactgttgga      660

ataggagcat atcttgctcg acttggcata cggtgcattc agcgtactga ccagcccatt      720

atcctaactg ggttttctgc cttgaacaag cttcttggcc gggaagtgta cagctcccac      780

atgcagttgg gtggccccaa aattatggcc acaaacggtg ttgtccatct gacagtttca      840

gatgaccttg aaggtgtatc taatatattg aggtggctca gctatgttcc tgccaacatt      900

ggtggacctc ttcctattac aaaatctttg gacccacctg acagacccgt tgcttacatc      960

cctgagaata catgtgatcc tcgtgcagcc atcagtggca ttgatgatag ccaagggaaa     1020

tggttggggg gcatgttcga caaagacagt tttgtggaga catttgaagg atgggcgaag     1080

tcagtagtta ctggcagagc gaaactcgga gggattccgg tgggtgttat agctgtggag     1140

acacagacta tgatgcagct catccctgct gatccaggtc agcttgattc ccatgagcgg     1200

tctgttcctc gtgctgggca agtctggttt ccagattcag ctactaagac agcgcaggca     1260

atgctggact tcaaccgtga aggattacct ctgttcatcc ttgctaactg gagaggcttc     1320

tctggtgggc aaagagatct ttttgaagga atccttcagg ctgggtcaac aattgttgag     1380

aaccttagga catacaatca gcctgccttt gtatatatcc ccaaggctgc agagctacgt     1440

ggaggggctt gggtcgtgat tgatagcaag ataaatccag atcgcattga gttctatgct     1500

gagaggactg caaagggcaa tgttcttgaa cctcaagggt tgattgagat caagttcagg     1560

tcagaggaac tccaagagtg catgggcagg cttgacccag aattgataaa tttgaaggca     1620

aaactcctgg gagcaaagca tgaaaatgga agtctatctg agtcagaatc ccttcagaag     1680

agcatagaag cccggaagaa acagttgttg cctttgtata ctcaaattgc ggtacggttc     1740

gctgaattgc atgacacttc ccttagaatg gctgctaagg gtgtgattaa gaaggttgta     1800

gactgggaag attctaggtc tttcttctac aagagattac ggaggaggat atccgaggat     1860

gttcttgcaa aggaaattag aggtgtaagt ggcaagcagt tttctcacca atcggcaatc     1920

gagctgatcc agaaatggta cttggcctct aagggagctg aaacaggaaa cactgaatgg     1980

gatgatgacg atgcttttgt tgcctggagg gaaaaccctg aaaactacca ggagtatatc     2040

aaagaactca gggctcaaag ggtatctcag ttgctctcag atgttgcaga ctccagtcca     2100

gatctagaag ccttgccaca gggtctttct atgctactag agaag                     2145


<210>  33
<211>  362
<212>  DNA
<213>  Triticum aestivum

<400>  33
gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta tcaaggagca       60

cattgttact cactgctagg agggaatcga actaggaata ttgatcagag gaactacgag      120

agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt gagatgcagc      180

ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat cgggcttttg      240

tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc ttagtttagt      300

cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc tgcatcagac      360

tt                                                                     362


<210>  34
<211>  527
<212>  DNA
<213>  Artificial

<220>
<223>  TaU6_pegRNA_1275rev

<400>  34
gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta tcaaggagca       60

cattgttact cactgctagg agggaatcga actaggaata ttgatcagag gaactacgag      120

agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt gagatgcagc      180

ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat cgggcttttg      240

tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc ttagtttagt      300

cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc tgcatcagac      360

ttgtgaacag aggtaatcct tcagttttag agctagaaat agcaagttaa aataaggcta      420

gtccgttatc aacttgaaaa agtggcaccg agtcggtgca gattcagtta ctaagacagc      480

tcaagcaatg ctggacttca accgtgaagg attacctctg ttttttt                    527


<210>  35
<211>  465
<212>  DNA
<213>  Artificial

<220>
<223>  TaU6_gRNA_1178forw

<400>  35
gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta tcaaggagca       60

cattgttact cactgctagg agggaatcga actaggaata ttgatcagag gaactacgag      120

agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt gagatgcagc      180

ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat cgggcttttg      240

tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc ttagtttagt      300

cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc tgcatcagac      360

ttgccagctt gattcccatg aggttttaga gctagaaata gcaagttaaa ataaggctag      420

tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttt                      465


<210>  36
<211>  466
<212>  DNA
<213>  Artificial

<220>
<223>  TaU6_pegRNA_1205forw

<400>  36
gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta tcaaggagca       60

cattgttact cactgctagg agggaatcga actaggaata ttgatcagag gaactacgag      120

agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt gagatgcagc      180

ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat cgggcttttg      240

tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc ttagtttagt      300

cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc tgcatcagac      360

ttgttcctcg tgctgggcaa gtcgttttag agctagaaat agcaagttaa aataaggcta      420

gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttt                     466


<210>  37
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  TaACCase_forw

<400>  37
atgatgcagc tcatccctg                                                    19


<210>  38
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  TaACCase_rev

<400>  38
ttgttgaccc agcctgaag                                                    19


<210>  39
<211>  1368
<212>  PRT
<213>  Artificial

<220>
<223>  nCas9(H840A)

<400>  39

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  40
<211>  2117
<212>  PRT
<213>  Artificial

<220>
<223>  PE2 editor

<400>  40

Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
            20                  25                  30          


Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys 
        35                  40                  45              


Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn 
    50                  55                  60                  


Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr 
65                  70                  75                  80  


Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg 
                85                  90                  95      


Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp 
            100                 105                 110         


Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp 
        115                 120                 125             


Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val 
    130                 135                 140                 


Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu 
145                 150                 155                 160 


Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
                165                 170                 175     


Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu 
            180                 185                 190         


Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln 
        195                 200                 205             


Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val 
    210                 215                 220                 


Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu 
225                 230                 235                 240 


Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe 
                245                 250                 255     


Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser 
            260                 265                 270         


Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr 
        275                 280                 285             


Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr 
    290                 295                 300                 


Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
305                 310                 315                 320 


Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser 
                325                 330                 335     


Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu 
            340                 345                 350         


Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile 
        355                 360                 365             


Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly 
    370                 375                 380                 


Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys 
385                 390                 395                 400 


Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu 
                405                 410                 415     


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr 
        435                 440                 445             


Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe 
465                 470                 475                 480 


Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe 
                485                 490                 495     


Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg 
            500                 505                 510         


Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys 
        515                 520                 525             


His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys 
    530                 535                 540                 


Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly 
545                 550                 555                 560 


Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys 
                565                 570                 575     


Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys 
            580                 585                 590         


Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser 
        595                 600                 605             


Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe 
    610                 615                 620                 


Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr 
625                 630                 635                 640 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr 
                645                 650                 655     


Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg 
            660                 665                 670         


Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile 
        675                 680                 685             


Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp 
    690                 695                 700                 


Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu 
705                 710                 715                 720 


Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp 
                725                 730                 735     


Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys 
            740                 745                 750         


Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val 
        755                 760                 765             


Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu 
    770                 775                 780                 


Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys 
785                 790                 795                 800 


Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu 
                805                 810                 815     


His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr 
            820                 825                 830         


Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile 
        835                 840                 845             


Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe 
    850                 855                 860                 


Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys 
865                 870                 875                 880 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys 
                885                 890                 895     


Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln 
            900                 905                 910         


Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu 
        915                 920                 925             


Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys 
945                 950                 955                 960 


Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu 
                965                 970                 975     


Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys 
            980                 985                 990         


Val Arg Glu Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1010                 1015                 1020             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1025                 1030                 1035             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1040                 1045                 1050             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1055                 1060                 1065             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1070                 1075                 1080             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1085                 1090                 1095             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1100                 1105                 1110             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1115                 1120                 1125             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1130                 1135                 1140             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1145                 1150                 1155             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1160                 1165                 1170             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1175                 1180                 1185             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1190                 1195                 1200             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1205                 1210                 1215             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1220                 1225                 1230             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1235                 1240                 1245             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1250                 1255                 1260             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1265                 1270                 1275             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1280                 1285                 1290             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1295                 1300                 1305             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1310                 1315                 1320             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1325                 1330                 1335             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1340                 1345                 1350             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1355                 1360                 1365             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1370                 1375                 1380             


Gly Gly  Asp Ser Gly Gly Ser  Ser Gly Gly Ser Ser  Gly Ser Glu 
    1385                 1390                 1395             


Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Gly 
    1400                 1405                 1410             


Ser Ser  Gly Gly Ser Ser Thr  Leu Asn Ile Glu Asp  Glu Tyr Arg 
    1415                 1420                 1425             


Leu His  Glu Thr Ser Lys Glu  Pro Asp Val Ser Leu  Gly Ser Thr 
    1430                 1435                 1440             


Trp Leu  Ser Asp Phe Pro Gln  Ala Trp Ala Glu Thr  Gly Gly Met 
    1445                 1450                 1455             


Gly Leu  Ala Val Arg Gln Ala  Pro Leu Ile Ile Pro  Leu Lys Ala 
    1460                 1465                 1470             


Thr Ser  Thr Pro Val Ser Ile  Lys Gln Tyr Pro Met  Ser Gln Glu 
    1475                 1480                 1485             


Ala Arg  Leu Gly Ile Lys Pro  His Ile Gln Arg Leu  Leu Asp Gln 
    1490                 1495                 1500             


Gly Ile  Leu Val Pro Cys Gln  Ser Pro Trp Asn Thr  Pro Leu Leu 
    1505                 1510                 1515             


Pro Val  Lys Lys Pro Gly Thr  Asn Asp Tyr Arg Pro  Val Gln Asp 
    1520                 1525                 1530             


Leu Arg  Glu Val Asn Lys Arg  Val Glu Asp Ile His  Pro Thr Val 
    1535                 1540                 1545             


Pro Asn  Pro Tyr Asn Leu Leu  Ser Gly Leu Pro Pro  Ser His Gln 
    1550                 1555                 1560             


Trp Tyr  Thr Val Leu Asp Leu  Lys Asp Ala Phe Phe  Cys Leu Arg 
    1565                 1570                 1575             


Leu His  Pro Thr Ser Gln Pro  Leu Phe Ala Phe Glu  Trp Arg Asp 
    1580                 1585                 1590             


Pro Glu  Met Gly Ile Ser Gly  Gln Leu Thr Trp Thr  Arg Leu Pro 
    1595                 1600                 1605             


Gln Gly  Phe Lys Asn Ser Pro  Thr Leu Phe Asn Glu  Ala Leu His 
    1610                 1615                 1620             


Arg Asp  Leu Ala Asp Phe Arg  Ile Gln His Pro Asp  Leu Ile Leu 
    1625                 1630                 1635             


Leu Gln  Tyr Val Asp Asp Leu  Leu Leu Ala Ala Thr  Ser Glu Leu 
    1640                 1645                 1650             


Asp Cys  Gln Gln Gly Thr Arg  Ala Leu Leu Gln Thr  Leu Gly Asn 
    1655                 1660                 1665             


Leu Gly  Tyr Arg Ala Ser Ala  Lys Lys Ala Gln Ile  Cys Gln Lys 
    1670                 1675                 1680             


Gln Val  Lys Tyr Leu Gly Tyr  Leu Leu Lys Glu Gly  Gln Arg Trp 
    1685                 1690                 1695             


Leu Thr  Glu Ala Arg Lys Glu  Thr Val Met Gly Gln  Pro Thr Pro 
    1700                 1705                 1710             


Lys Thr  Pro Arg Gln Leu Arg  Glu Phe Leu Gly Lys  Ala Gly Phe 
    1715                 1720                 1725             


Cys Arg  Leu Phe Ile Pro Gly  Phe Ala Glu Met Ala  Ala Pro Leu 
    1730                 1735                 1740             


Tyr Pro  Leu Thr Lys Pro Gly  Thr Leu Phe Asn Trp  Gly Pro Asp 
    1745                 1750                 1755             


Gln Gln  Lys Ala Tyr Gln Glu  Ile Lys Gln Ala Leu  Leu Thr Ala 
    1760                 1765                 1770             


Pro Ala  Leu Gly Leu Pro Asp  Leu Thr Lys Pro Phe  Glu Leu Phe 
    1775                 1780                 1785             


Val Asp  Glu Lys Gln Gly Tyr  Ala Lys Gly Val Leu  Thr Gln Lys 
    1790                 1795                 1800             


Leu Gly  Pro Trp Arg Arg Pro  Val Ala Tyr Leu Ser  Lys Lys Leu 
    1805                 1810                 1815             


Asp Pro  Val Ala Ala Gly Trp  Pro Pro Cys Leu Arg  Met Val Ala 
    1820                 1825                 1830             


Ala Ile  Ala Val Leu Thr Lys  Asp Ala Gly Lys Leu  Thr Met Gly 
    1835                 1840                 1845             


Gln Pro  Leu Val Ile Leu Ala  Pro His Ala Val Glu  Ala Leu Val 
    1850                 1855                 1860             


Lys Gln  Pro Pro Asp Arg Trp  Leu Ser Asn Ala Arg  Met Thr His 
    1865                 1870                 1875             


Tyr Gln  Ala Leu Leu Leu Asp  Thr Asp Arg Val Gln  Phe Gly Pro 
    1880                 1885                 1890             


Val Val  Ala Leu Asn Pro Ala  Thr Leu Leu Pro Leu  Pro Glu Glu 
    1895                 1900                 1905             


Gly Leu  Gln His Asn Cys Leu  Asp Ile Leu Ala Glu  Ala His Gly 
    1910                 1915                 1920             


Thr Arg  Pro Asp Leu Thr Asp  Gln Pro Leu Pro Asp  Ala Asp His 
    1925                 1930                 1935             


Thr Trp  Tyr Thr Asp Gly Ser  Ser Leu Leu Gln Glu  Gly Gln Arg 
    1940                 1945                 1950             


Lys Ala  Gly Ala Ala Val Thr  Thr Glu Thr Glu Val  Ile Trp Ala 
    1955                 1960                 1965             


Lys Ala  Leu Pro Ala Gly Thr  Ser Ala Gln Arg Ala  Glu Leu Ile 
    1970                 1975                 1980             


Ala Leu  Thr Gln Ala Leu Lys  Met Ala Glu Gly Lys  Lys Leu Asn 
    1985                 1990                 1995             


Val Tyr  Thr Asp Ser Arg Tyr  Ala Phe Ala Thr Ala  His Ile His 
    2000                 2005                 2010             


Gly Glu  Ile Tyr Arg Arg Arg  Gly Trp Leu Thr Ser  Glu Gly Lys 
    2015                 2020                 2025             


Glu Ile  Lys Asn Lys Asp Glu  Ile Leu Ala Leu Leu  Lys Ala Leu 
    2030                 2035                 2040             


Phe Leu  Pro Lys Arg Leu Ser  Ile Ile His Cys Pro  Gly His Gln 
    2045                 2050                 2055             


Lys Gly  His Ser Ala Glu Ala  Arg Gly Asn Arg Met  Ala Asp Gln 
    2060                 2065                 2070             


Ala Ala  Arg Lys Ala Ala Ile  Thr Glu Thr Pro Asp  Thr Ser Thr 
    2075                 2080                 2085             


Leu Leu  Ile Glu Asn Ser Ser  Pro Ser Gly Gly Ser  Lys Arg Thr 
    2090                 2095                 2100             


Ala Asp  Gly Ser Glu Phe Glu  Pro Lys Lys Lys Arg  Lys Val 
    2105                 2110                 2115         


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for pegRNA used to modify ZmALS2

<400>  41
catggtcgcc atcacgggac                                                   20


<210>  42
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  RT-template of pegRNA used to modify ZmALS2

<400>  42
gtcgcgacac ctgtc                                                        15


<210>  43
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Binding Site of pegRNA used to modify ZmALS2

<400>  43
ccgtgatggc gac                                                          13


<210>  44
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for pegRNA used to modify ZmALS2

<400>  44
tcggtgccaa tcatgcgtcg                                                   20


<210>  45
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  RT-template of pegRNA used to modify ZmALS2

<400>  45
acaggtgtcg cga                                                          13


<210>  46
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Binding Site of pegRNA used to modify ZmALS2

<400>  46
cgcatgattg gca                                                          13


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for pegRNA used to modify ZmALS2

<400>  47
tccttgaaag ccccaccact                                                   20


<210>  48
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  RT-template of pegRNA used to modify ZmALS2

<400>  48
tatgatccct aat                                                          13


<210>  49
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Binding Site of pegRNA used to modify ZmALS2

<400>  49
ggtggggctt tca                                                          13


<210>  50
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for pegRNA used to modify ZmALS2

<400>  50
gtgttgccta tgatccctag                                                   20


<210>  51
<211>  12
<212>  DNA
<213>  Artificial

<220>
<223>  RT-template of pegRNA used to modify ZmALS2

<400>  51
gccccaccat ta                                                           12


<210>  52
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Binding Site of pegRNA used to modify ZmALS2

<400>  52
gggatcatag gca                                                          13


<210>  53
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify ZmALS2

<400>  53
gacggaatcg agcagcgcgt                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify ZmALS2

<400>  54
ccaggagacg cccatcgtcg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify ZmALS2

<400>  55
aagaagatgc tcgagactcc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify ZmALS2

<400>  56
gtgggacgat tatatccaag                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for pegRNA used to modify TaACCase

<400>  57
tgaacagagg taatccttca                                                   20


<210>  58
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  RT-template of pegRNA used to modify TaACCase

<400>  58
agattcagtt actaagacag ctcaagcaat gctggacttc aaccgtga                    48


<210>  59
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Binding Site of pegRNA used to modify TaACCase

<400>  59
aggattacct ctg                                                          13


<210>  60
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify TaACCase

<400>  60
gccagcttga ttcccatgag                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer for gRNA used to modify TaACCase

<400>  61
ttcctcgtgc tgggcaagtc                                                   20


<210>  62
<211>  2404
<212>  DNA
<213>  Physcomitrella patens

<400>  62
gccgtggcgc atcttgtgga cttgccgtgg cgagctacct ccacgctccc gcttccgcaa       60

ttttgttgca gtgctttgga ttctcgtggt tgctcgtctc aggtggttca cttcttaact      120

tgcttcgtta cgcgccgcct tctcctgcat tgtctcttca tatatatgta tatatttttt      180

tttcttttca cttttgttga gctcggtgag ggtatctagt tttggtgaat aaatgtgcag      240

agtgtagaat ttgctgtggg ttatgggcac agtgcctgtg atgcggttgt tgttgttgtt      300

gttggagaag ccctttgttt tgggctcgag acttgaataa aatatgggac ggtactggtt      360

ccattttgta gaattatgat tatgatgatg gtcaatgtgg cagcaagtag gaggattgga      420

tttgaattgt gggtttttgc gctcgctgtt tctgagttag ttactgacga agctctgtaa      480

attgtgtgtg gacaggaagt gaagatgtcg gcctccaagg atggcgaccc tcggatccag      540

tatatctcgg acagcatccg taccattcct gattttcctc acaaaggtac tatgctccac      600

gccatagtcg tcactctcgg ttttgttttg cagtgttcgt gacatgtggt ttttttcttt      660

tttcaccttt agcgaaggtt ttgtatggaa ttctctttcg tagcgcgagt ttacgtgatg      720

aatttggtgc aggcattatg ttccgagatg tgacgacgtt gctgttggat cataaggctt      780

tcaaagacac gatcgacatc tttgttgagc gttaccggga ccagaaggtg gacgtcattg      840

tgggtgcgat gcccttgact cccttgacgt accataacgg ttttgtgtaa taatttgaag      900

gcaaccgtta cgtttataaa tttgggaaga ttgtctggct gttgttgatt actttcgtgc      960

tacttttttc aggaattgaa gctcgaggtt ttatctttgg gccacccatt gctcttgcca     1020

tcggtgcgaa gtttgtgcca atgcgcaagc ctcgtaaact tccaggtaag ggttgacagg     1080

cactttttct cttaatctcg tgattcatga aagattaggg ctctcaatgg ctttcgtcgt     1140

ccgtggaata gagttttcaa tgtgaccgag acttcatcct catgtagctt aaaggtgtat     1200

tcgctttcta tttctcaagc tgcgaaagca tgtcagcact tattatctag agaagctaaa     1260

tacattattt caacattttt gtgatatcga ttgctttgta tgtgctttgc atactaagaa     1320

tcaaaatcct tgacaatttt gtgctgacgt ccgtgttcac atgtcttagg ccctgtgatt     1380

agggaagagt atagtctaga gtatggtacc gattgcattg agatgcacgt tggagccatc     1440

gagaagaacg agcgcgtagt tattgttgat gacttaattg ccaccggagg cactcttgga     1500

gctgccatca aattgctagg tttgctttct ttcttcctct cttacttaaa tgagaactgt     1560

tttcgaacat ttaagcagga aactttgtct tcaacaacct gtgaagcatg gcgttttgtt     1620

tagacgttgg atccaactac aggaagaaac ctaaatgtgt gttttcatgt tagcactagt     1680

tttgtcgaat ctttgagatg tttgaccttg aaagttgaca ccaccttgtt taacagacaa     1740

agttgaagcg gagattgtgg aatgcgcgtg cttggttgag ctggtagacc tgaaggtatt     1800

tttctcccac tgcataatct gtagggttgc tttctctgag gcaattgtaa gagttcaatg     1860

ccgtctggag cttacagttt tatcgtttat cctatgatga cgtagaataa ccgaaaatcg     1920

taattatctc cagcaagact cctctttttg tgtgttgtta acacatcttc attcttagct     1980

acaaagagtt taatgccgtc tagtatctgc tatgttgatg aacttggaac atatttgcag     2040

ggtcgtgaga agttgtcggg caagccgctg tacatcttgg tggaatatga gggcgagtaa     2100

gcttcaccat ccccgaactg tcgccgtggt cattggttct atgaagtgcc aatcctatat     2160

atatggaaac acgatctgca aacattatgg caaaagcaga tcgtactgca attaattttg     2220

agctactatc gattctttga ttttcggttt tccaaggtgt atgactttgg gtcagaatta     2280

tttagacaac ctggttgtct gaacattcat atatacttat ctcagaggat tatgctttac     2340

tgggaccttt tcttctaacc tcggaaacgt aattgtagca tcacctactc ctgcattgca     2400

acag                                                                  2404


<210>  63
<211>  184
<212>  PRT
<213>  Physcomitrella patens

<400>  63

Met Ser Ala Ser Lys Asp Gly Asp Pro Arg Ile Gln Tyr Ile Ser Asp 
1               5                   10                  15      


Ser Ile Arg Thr Ile Pro Asp Phe Pro His Lys Gly Ile Met Phe Arg 
            20                  25                  30          


Asp Val Thr Thr Leu Leu Leu Asp His Lys Ala Phe Lys Asp Thr Ile 
        35                  40                  45              


Asp Ile Phe Val Glu Arg Tyr Arg Asp Gln Lys Val Asp Val Ile Val 
    50                  55                  60                  


Gly Ile Glu Ala Arg Gly Phe Ile Phe Gly Pro Pro Ile Ala Leu Ala 
65                  70                  75                  80  


Ile Gly Ala Lys Phe Val Pro Met Arg Lys Pro Arg Lys Leu Pro Gly 
                85                  90                  95      


Pro Val Ile Arg Glu Glu Tyr Ser Leu Glu Tyr Gly Thr Asp Cys Ile 
            100                 105                 110         


Glu Met His Val Gly Ala Ile Glu Lys Asn Glu Arg Val Val Ile Val 
        115                 120                 125             


Asp Asp Leu Ile Ala Thr Gly Gly Thr Leu Gly Ala Ala Ile Lys Leu 
    130                 135                 140                 


Leu Asp Lys Val Glu Ala Glu Ile Val Glu Cys Ala Cys Leu Val Glu 
145                 150                 155                 160 


Leu Val Asp Leu Lys Gly Arg Glu Lys Leu Ser Gly Lys Pro Leu Tyr 
                165                 170                 175     


Ile Leu Val Glu Tyr Glu Gly Glu 
            180                 


<210>  64
<211>  968
<212>  PRT
<213>  Oryza sativa

<400>  64

Pro Leu Leu Leu Thr Ala Ser Thr Ser Ile Pro Lys Ser Lys Thr Phe 
1               5                   10                  15      


Arg Phe Glu Asn His Leu Leu Leu Asp Arg Asp Phe Leu Pro Ser Val 
            20                  25                  30          


Met His Arg Trp Pro Gln Gln Leu Asn Met Asn Asp Pro Ala Ala Cys 
        35                  40                  45              


Leu Ala Ala Lys Leu Lys Gln Thr Arg Ser Ile Ile Lys Val Trp Met 
    50                  55                  60                  


Lys Glu His Arg Lys Arg Ser Ser Leu Asn Glu Asp Cys Lys Phe Thr 
65                  70                  75                  80  


Ile Asp Leu Leu Asp Tyr Ile Glu Glu Leu Arg Pro Leu Asn Asp Gly 
                85                  90                  95      


Glu Arg Cys Leu Arg Asn Leu Val Gln Glu Lys Leu Phe Gln Ala Ile 
            100                 105                 110         


Gln Ser Lys Val Ala Tyr Trp Lys Gln Arg Gly Lys Val Lys Arg Leu 
        115                 120                 125             


Lys Ile Gly Ile Asp Asn Ser Ala Phe Phe Lys Ala His Ala Ser Lys 
    130                 135                 140                 


Asn Tyr Arg Asn Ser Arg Ile Arg Ser Ile Lys His Asn Gly Ala Glu 
145                 150                 155                 160 


Ile Ala Asp His Asn Gly Lys Ala Leu Leu Leu His Ser Phe Phe Gln 
                165                 170                 175     


His Phe Leu Gly Ser Asp Cys Gln Pro Leu Trp Asp Phe Asp Leu Ser 
            180                 185                 190         


Thr Leu Tyr Ser Glu Asp Glu Arg Asp Tyr Asn Leu Leu Ala Ser Gln 
        195                 200                 205             


Gly Leu Gln Phe Ser Thr Glu Glu Ile Ile Thr Ser Ile Lys Gly Met 
    210                 215                 220                 


Asn Ser Asn Ser Ala Pro Gly Pro Asp Gly Phe Gly Pro Ser Phe Tyr 
225                 230                 235                 240 


Asn Ser Ala Trp Asn Ser Ile Lys Glu Asp Ile Ile Lys Leu Ala Ala 
                245                 250                 255     


Ser Phe Cys Ser Asn Ser Val Gln Leu Glu Arg Ile Asn Arg Ala His 
            260                 265                 270         


Ile Val Leu Ile Pro Lys Pro Gly Lys Glu Asn Thr Val Asp Gly Tyr 
        275                 280                 285             


Arg Pro Ile Ser Leu Gln Asn Cys Ser Val Lys Ile Leu Ser Lys Val 
    290                 295                 300                 


Leu Ala Asn Arg Leu Gln Lys Val Leu Thr Arg Met Ile His Leu Asp 
305                 310                 315                 320 


Gln Thr Gly Phe Leu Lys Gly Arg Cys Ile Ser Glu Asn Leu Ile Tyr 
                325                 330                 335     


Ala Thr Glu Leu Ile Gln Ala Cys His Ala Arg Lys Cys Lys Thr Leu 
            340                 345                 350         


Ile Ile Lys Leu Asp Phe Ala Lys Ala Phe Asp Ser Val Leu Trp Thr 
        355                 360                 365             


Ser Leu Phe Gln Ile Leu Ala Val Arg Gly Phe Pro Asn Asn Trp Ile 
    370                 375                 380                 


Ser Trp Ile Lys Ser Leu Leu Gln Thr Ser Lys Ser Ala Val Leu Leu 
385                 390                 395                 400 


Asn Gly Ile Pro Gly Lys Trp Ile Asn Cys Lys Lys Gly Leu Arg Gln 
                405                 410                 415     


Gly Asp Pro Leu Ser Pro Tyr Leu Phe Ile Leu Val Ala Asp Val Leu 
            420                 425                 430         


Gln Arg Leu Leu Glu Lys Asn Phe Gln Ile Arg His Pro Ile Tyr Gln 
        435                 440                 445             


Asp Arg Pro Cys Ala Thr Ile Gln Tyr Ala Asp Asp Thr Leu Val Ile 
    450                 455                 460                 


Cys Arg Ala Glu Glu Asp Asp Val Leu Ala Leu Arg Ser Thr Leu Leu 
465                 470                 475                 480 


Gln Phe Ser Lys Ala Thr Gly Leu Gln Ile Asn Phe Ala Lys Ser Thr 
                485                 490                 495     


Met Ile Ser Leu His Ile Asp Arg Ser Lys Glu Ser Ser Leu Ser Glu 
            500                 505                 510         


Leu Leu Gln Cys Lys Leu Glu Ser Leu Pro Met Ser Tyr Leu Gly Leu 
        515                 520                 525             


Pro Leu Ser Leu His Lys Leu Thr Asn Asn Asp Leu Gln Pro Ile Val 
    530                 535                 540                 


Val Lys Val Asp Ser Phe Leu Thr Gly Trp Glu Ala Ser Leu Leu Ser 
545                 550                 555                 560 


Gln Ala Glu Arg Leu Ile Leu Val Asn Ala Val Leu Ser Ser Val Pro 
                565                 570                 575     


Val Tyr Ala Met Ser Ala Phe Lys Leu Pro Pro Lys Val Ile Glu Ala 
            580                 585                 590         


Ile Asp Lys Arg Arg Arg Ala Phe Phe Trp Thr Gly Asp Asp Thr Cys 
        595                 600                 605             


Ser Gly Ala Lys Cys Leu Val Ala Trp Asp Glu Val Cys Thr Ala Lys 
    610                 615                 620                 


Glu Lys Gly Gly Leu Gly Ile Lys Ser Leu Lys Thr Gln Asn Glu Ala 
625                 630                 635                 640 


Leu Leu Leu Lys Arg Leu Phe Asn Leu Phe Ser Asp Asn Ser Ser Trp 
                645                 650                 655     


Thr Asn Trp Ile Trp Lys Glu Phe Asp Gly Arg Ser Leu Leu Lys Ser 
            660                 665                 670         


Leu Pro Leu Gly Gln His Trp Ser Val Phe Gln Lys Leu Leu Pro Glu 
        675                 680                 685             


Leu Phe Lys Ile Thr Thr Val His Ile Gly Asp Gly Ser Arg Thr Ser 
    690                 695                 700                 


Phe Trp His Asp Arg Trp Thr Gly Asn Met Thr Leu Ala Ala Gln Phe 
705                 710                 715                 720 


Glu Ser Leu Phe Ser His Ser Thr Asp Asp Leu Ala Thr Val Glu Gln 
                725                 730                 735     


Ile Leu Ser Phe Gly Ile Tyr Asp Ile Leu Thr Pro Arg Leu Ser Ser 
            740                 745                 750         


Ala Ala Gln Asn Asp Leu Gln Glu Leu Gln Thr Ile Leu Gln Asn Phe 
        755                 760                 765             


Ser Leu Thr Asn Glu Ser Asp Thr Arg Leu Asn Asn Arg Leu Gly Asn 
    770                 775                 780                 


Leu Thr Thr Lys Thr Ile Tyr Asp Leu Arg Ser Pro Pro Gly Phe Pro 
785                 790                 795                 800 


Ser Pro Asn Trp Lys Phe Ile Trp Asp Cys Arg Met Pro Leu Lys Ile 
                805                 810                 815     


Lys Leu Phe Ala Trp Leu Leu Val Arg Asp Arg Leu Ser Thr Lys Leu 
            820                 825                 830         


Asn Leu Leu Lys Lys Lys Ile Val Gln Thr Ala Thr Cys Asp Ile Cys 
        835                 840                 845             


Ser Thr Thr Asp Glu Thr Ala Asp His Leu Ser Phe Asn Cys Pro Phe 
    850                 855                 860                 


Ala Ile Ser Phe Trp Gln Ala Leu His Ile Gln Pro Ile Ile Asn Glu 
865                 870                 875                 880 


Thr Lys Tyr Leu Ser Gln Leu Lys Ala Pro Ala Thr Ile Pro Thr Arg 
                885                 890                 895     


His Phe Gln Ser Phe Phe Met Leu Cys Phe Trp Val Leu Trp Asn His 
            900                 905                 910         


Arg His Asp Val Val Phe Arg Gly Arg Pro Pro Ser Ile Ala Ser Cys 
        915                 920                 925             


Leu Gln Arg Gly Ile Ser Glu Ser Ser Leu Trp Ala Glu Thr Phe Thr 
    930                 935                 940                 


Pro Asp Asp Arg Phe Val Ile Asp Val Trp Lys Gly Val Phe Ser Ser 
945                 950                 955                 960 


Ser Leu Gln Thr Leu His Pro Met 
                965             


<210>  65
<211>  2407
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nCas9-KarmaRT

<400>  65

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Ser Gly Gly Ser Ser  Gly Gly Ser Ser Gly  Ser Glu Thr 
    1385                 1390                 1395             


Pro Gly  Thr Ser Glu Ser Ala  Thr Pro Glu Ser Ser  Gly Gly Ser 
    1400                 1405                 1410             


Ser Gly  Gly Ser Ser Pro Leu  Leu Leu Thr Ala Ser  Thr Ser Ile 
    1415                 1420                 1425             


Pro Lys  Ser Lys Thr Phe Arg  Phe Glu Asn His Leu  Leu Leu Asp 
    1430                 1435                 1440             


Arg Asp  Phe Leu Pro Ser Val  Met His Arg Trp Pro  Gln Gln Leu 
    1445                 1450                 1455             


Asn Met  Asn Asp Pro Ala Ala  Cys Leu Ala Ala Lys  Leu Lys Gln 
    1460                 1465                 1470             


Thr Arg  Ser Ile Ile Lys Val  Trp Met Lys Glu His  Arg Lys Arg 
    1475                 1480                 1485             


Ser Ser  Leu Asn Glu Asp Cys  Lys Phe Thr Ile Asp  Leu Leu Asp 
    1490                 1495                 1500             


Tyr Ile  Glu Glu Leu Arg Pro  Leu Asn Asp Gly Glu  Arg Cys Leu 
    1505                 1510                 1515             


Arg Asn  Leu Val Gln Glu Lys  Leu Phe Gln Ala Ile  Gln Ser Lys 
    1520                 1525                 1530             


Val Ala  Tyr Trp Lys Gln Arg  Gly Lys Val Lys Arg  Leu Lys Ile 
    1535                 1540                 1545             


Gly Ile  Asp Asn Ser Ala Phe  Phe Lys Ala His Ala  Ser Lys Asn 
    1550                 1555                 1560             


Tyr Arg  Asn Ser Arg Ile Arg  Ser Ile Lys His Asn  Gly Ala Glu 
    1565                 1570                 1575             


Ile Ala  Asp His Asn Gly Lys  Ala Leu Leu Leu His  Ser Phe Phe 
    1580                 1585                 1590             


Gln His  Phe Leu Gly Ser Asp  Cys Gln Pro Leu Trp  Asp Phe Asp 
    1595                 1600                 1605             


Leu Ser  Thr Leu Tyr Ser Glu  Asp Glu Arg Asp Tyr  Asn Leu Leu 
    1610                 1615                 1620             


Ala Ser  Gln Gly Leu Gln Phe  Ser Thr Glu Glu Ile  Ile Thr Ser 
    1625                 1630                 1635             


Ile Lys  Gly Met Asn Ser Asn  Ser Ala Pro Gly Pro  Asp Gly Phe 
    1640                 1645                 1650             


Gly Pro  Ser Phe Tyr Asn Ser  Ala Trp Asn Ser Ile  Lys Glu Asp 
    1655                 1660                 1665             


Ile Ile  Lys Leu Ala Ala Ser  Phe Cys Ser Asn Ser  Val Gln Leu 
    1670                 1675                 1680             


Glu Arg  Ile Asn Arg Ala His  Ile Val Leu Ile Pro  Lys Pro Gly 
    1685                 1690                 1695             


Lys Glu  Asn Thr Val Asp Gly  Tyr Arg Pro Ile Ser  Leu Gln Asn 
    1700                 1705                 1710             


Cys Ser  Val Lys Ile Leu Ser  Lys Val Leu Ala Asn  Arg Leu Gln 
    1715                 1720                 1725             


Lys Val  Leu Thr Arg Met Ile  His Leu Asp Gln Thr  Gly Phe Leu 
    1730                 1735                 1740             


Lys Gly  Arg Cys Ile Ser Glu  Asn Leu Ile Tyr Ala  Thr Glu Leu 
    1745                 1750                 1755             


Ile Gln  Ala Cys His Ala Arg  Lys Cys Lys Thr Leu  Ile Ile Lys 
    1760                 1765                 1770             


Leu Asp  Phe Ala Lys Ala Phe  Asp Ser Val Leu Trp  Thr Ser Leu 
    1775                 1780                 1785             


Phe Gln  Ile Leu Ala Val Arg  Gly Phe Pro Asn Asn  Trp Ile Ser 
    1790                 1795                 1800             


Trp Ile  Lys Ser Leu Leu Gln  Thr Ser Lys Ser Ala  Val Leu Leu 
    1805                 1810                 1815             


Asn Gly  Ile Pro Gly Lys Trp  Ile Asn Cys Lys Lys  Gly Leu Arg 
    1820                 1825                 1830             


Gln Gly  Asp Pro Leu Ser Pro  Tyr Leu Phe Ile Leu  Val Ala Asp 
    1835                 1840                 1845             


Val Leu  Gln Arg Leu Leu Glu  Lys Asn Phe Gln Ile  Arg His Pro 
    1850                 1855                 1860             


Ile Tyr  Gln Asp Arg Pro Cys  Ala Thr Ile Gln Tyr  Ala Asp Asp 
    1865                 1870                 1875             


Thr Leu  Val Ile Cys Arg Ala  Glu Glu Asp Asp Val  Leu Ala Leu 
    1880                 1885                 1890             


Arg Ser  Thr Leu Leu Gln Phe  Ser Lys Ala Thr Gly  Leu Gln Ile 
    1895                 1900                 1905             


Asn Phe  Ala Lys Ser Thr Met  Ile Ser Leu His Ile  Asp Arg Ser 
    1910                 1915                 1920             


Lys Glu  Ser Ser Leu Ser Glu  Leu Leu Gln Cys Lys  Leu Glu Ser 
    1925                 1930                 1935             


Leu Pro  Met Ser Tyr Leu Gly  Leu Pro Leu Ser Leu  His Lys Leu 
    1940                 1945                 1950             


Thr Asn  Asn Asp Leu Gln Pro  Ile Val Val Lys Val  Asp Ser Phe 
    1955                 1960                 1965             


Leu Thr  Gly Trp Glu Ala Ser  Leu Leu Ser Gln Ala  Glu Arg Leu 
    1970                 1975                 1980             


Ile Leu  Val Asn Ala Val Leu  Ser Ser Val Pro Val  Tyr Ala Met 
    1985                 1990                 1995             


Ser Ala  Phe Lys Leu Pro Pro  Lys Val Ile Glu Ala  Ile Asp Lys 
    2000                 2005                 2010             


Arg Arg  Arg Ala Phe Phe Trp  Thr Gly Asp Asp Thr  Cys Ser Gly 
    2015                 2020                 2025             


Ala Lys  Cys Leu Val Ala Trp  Asp Glu Val Cys Thr  Ala Lys Glu 
    2030                 2035                 2040             


Lys Gly  Gly Leu Gly Ile Lys  Ser Leu Lys Thr Gln  Asn Glu Ala 
    2045                 2050                 2055             


Leu Leu  Leu Lys Arg Leu Phe  Asn Leu Phe Ser Asp  Asn Ser Ser 
    2060                 2065                 2070             


Trp Thr  Asn Trp Ile Trp Lys  Glu Phe Asp Gly Arg  Ser Leu Leu 
    2075                 2080                 2085             


Lys Ser  Leu Pro Leu Gly Gln  His Trp Ser Val Phe  Gln Lys Leu 
    2090                 2095                 2100             


Leu Pro  Glu Leu Phe Lys Ile  Thr Thr Val His Ile  Gly Asp Gly 
    2105                 2110                 2115             


Ser Arg  Thr Ser Phe Trp His  Asp Arg Trp Thr Gly  Asn Met Thr 
    2120                 2125                 2130             


Leu Ala  Ala Gln Phe Glu Ser  Leu Phe Ser His Ser  Thr Asp Asp 
    2135                 2140                 2145             


Leu Ala  Thr Val Glu Gln Ile  Leu Ser Phe Gly Ile  Tyr Asp Ile 
    2150                 2155                 2160             


Leu Thr  Pro Arg Leu Ser Ser  Ala Ala Gln Asn Asp  Leu Gln Glu 
    2165                 2170                 2175             


Leu Gln  Thr Ile Leu Gln Asn  Phe Ser Leu Thr Asn  Glu Ser Asp 
    2180                 2185                 2190             


Thr Arg  Leu Asn Asn Arg Leu  Gly Asn Leu Thr Thr  Lys Thr Ile 
    2195                 2200                 2205             


Tyr Asp  Leu Arg Ser Pro Pro  Gly Phe Pro Ser Pro  Asn Trp Lys 
    2210                 2215                 2220             


Phe Ile  Trp Asp Cys Arg Met  Pro Leu Lys Ile Lys  Leu Phe Ala 
    2225                 2230                 2235             


Trp Leu  Leu Val Arg Asp Arg  Leu Ser Thr Lys Leu  Asn Leu Leu 
    2240                 2245                 2250             


Lys Lys  Lys Ile Val Gln Thr  Ala Thr Cys Asp Ile  Cys Ser Thr 
    2255                 2260                 2265             


Thr Asp  Glu Thr Ala Asp His  Leu Ser Phe Asn Cys  Pro Phe Ala 
    2270                 2275                 2280             


Ile Ser  Phe Trp Gln Ala Leu  His Ile Gln Pro Ile  Ile Asn Glu 
    2285                 2290                 2295             


Thr Lys  Tyr Leu Ser Gln Leu  Lys Ala Pro Ala Thr  Ile Pro Thr 
    2300                 2305                 2310             


Arg His  Phe Gln Ser Phe Phe  Met Leu Cys Phe Trp  Val Leu Trp 
    2315                 2320                 2325             


Asn His  Arg His Asp Val Val  Phe Arg Gly Arg Pro  Pro Ser Ile 
    2330                 2335                 2340             


Ala Ser  Cys Leu Gln Arg Gly  Ile Ser Glu Ser Ser  Leu Trp Ala 
    2345                 2350                 2355             


Glu Thr  Phe Thr Pro Asp Asp  Arg Phe Val Ile Asp  Val Trp Lys 
    2360                 2365                 2370             


Gly Val  Phe Ser Ser Ser Leu  Gln Thr Leu His Pro  Met Ser Gly 
    2375                 2380                 2385             


Gly Ser  Lys Arg Thr Ala Asp  Gly Ser Glu Phe Glu  Pro Lys Lys 
    2390                 2395                 2400             


Lys Arg  Lys Val 
    2405         


<210>  66
<211>  300
<212>  DNA
<213>  Zea mays

<400>  66
gcatccaatc caatatggac gattggacgt ggaaggtttc ctgtgtcttc tacgatataa       60

taattggtcg ctgtcctgtg tggatgtttc ctgtgtcttc tacgatatat taatgtactt      120

ctgtctgttt cgtttcgtat gtacaatctt ccgggtatcg ttcatgtatc cctttgaata      180

ataacaaata aaatcgggtt tgtcacggta atctgaataa ccttttatta gccaaagttt      240

aaccaaaatt acgtattgca cgtaaaaatt aagctagttt ttggagagcc ggagacagac      300


<210>  67
<211>  6351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-MMLV_RTmut_PE2

<400>  67
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcaccctc     4260

aacatcgagg acgagtaccg cctgcacgag acgtccaagg agccggacgt gtccctcggc     4320

agcacgtggc tctcggactt cccccaggct tgggccgaga cgggcggcat gggcctcgcc     4380

gtgaggcagg cgccgctcat catcccgctg aaggccacct ccacccccgt cagcatcaag     4440

cagtacccga tgtcgcagga ggccaggctc ggcatcaagc cccacatcca gaggctcctg     4500

gaccagggca tcctggtccc ctgccagagc ccatggaaca ccccgctcct gccggtgaag     4560

aagccgggca ccaacgacta caggcccgtc caggacctca gggaggtgaa caagcgcgtc     4620

gaggacatcc accccacggt gccgaacccg tacaacctcc tgtcgggcct gccgcccagc     4680

caccagtggt acaccgtcct cgacctgaag gacgccttct tctgcctgcg gctccacccc     4740

acctcgcagc ccctcttcgc tttcgagtgg cgcgaccccg agatgggcat ctcgggccag     4800

ctcacctgga cgaggctgcc acagggcttc aagaacagcc cgaccctgtt caacgaggcg     4860

ctccacaggg acctggcgga cttccgcatc cagcacccgg acctcatcct cctgcagtat     4920

gtggacgacc tcctcctggc ggccacctcg gagctggact gccagcaggg caccagggcg     4980

ctgctccaga cgctcggcaa cctgggctac agggcttcgg cgaagaaggc ccagatctgc     5040

cagaagcagg tgaagtacct cggctacctg ctcaaggagg gccagcggtg gctgacggag     5100

gctaggaagg agacggtcat gggccagccc accccaaaga cgcccaggca gctcagggag     5160

ttcctgggca aggccggctt ctgccgcctc ttcatccccg gcttcgcgga gatggcggcg     5220

ccgctctacc cactgaccaa gccgggcacg ctgttcaact ggggccccga ccagcagaag     5280

gcctaccagg agatcaagca ggccctgctc acggctcccg cgctcggcct gcccgacctc     5340

acgaagccgt tcgagctgtt cgtggacgag aagcagggct acgcgaaggg cgtcctcacg     5400

cagaagctgg gcccctggcg caggcccgtg gcctacctct ccaagaagct cgaccccgtg     5460

gcggccggct ggccgccatg cctgaggatg gtggcggcca tcgccgtcct caccaaggac     5520

gcgggcaagc tgaccatggg ccagcccctc gtgatcctcg cgccgcacgc ggtggaggcc     5580

ctcgtcaagc agccgccaga caggtggctg agcaacgccc gcatgaccca ctaccaggcc     5640

ctgctcctgg acaccgacag ggtccagttc ggcccggtgg tggctctgaa ccccgccacc     5700

ctcctgccgc tccccgagga gggcctgcag cacaactgcc tcgacatcct cgcggaggcc     5760

cacggcaccc gcccggacct cacggaccag ccgctgcccg acgccgacca cacctggtac     5820

accgacggct ccagcctcct gcaggagggc cagaggaagg cgggcgctgc ggtgaccacg     5880

gagacggagg tcatctgggc caaggcgctc ccggcgggca cctccgctca gcgcgcggag     5940

ctcatcgccc tgacgcaggc gctcaagatg gccgagggca agaagctgaa cgtgtacacg     6000

gactcccgct acgccttcgc caccgctcac atccacggcg agatctacag gaggaggggc     6060

tggctgacct cggagggcaa ggagatcaag aacaaggacg agatcctcgc gctcctgaag     6120

gccctcttcc tgcccaagcg cctgtccatc atccactgcc ccggccacca gaagggccac     6180

tcggccgagg ccaggggcaa caggatggcg gaccaggcgg ccaggaaggc ggccatcacg     6240

gagacgccgg acaccagcac gctcctgatc gagaactcca gcccgtccgg cggcagcaag     6300

cgcacggcgg acggctcgga gttcgagccg aagaagaagc gcaaggtgtg a              6351


<210>  68
<211>  5862
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-CaMVRT

<400>  68
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagctccaag     4260

acccagcagc cggagcccgt gaacatcagc acgaacaaga tcgagaaccc cctcgaggag     4320

atcgccatcc tctcggaggg caggaggctc tcggaggaga agctgttcat cacccagcag     4380

cgcatgcaga agacggagga gctcctggag aaggtgtgct ccgagaaccc gctcgacccc     4440

aacaagacca agcagtggat gaaggcgagc atcaagctgt ccgacccgag caaggccatc     4500

aaggtcaagc ccatgaagta ctccccgatg gacagggagg agttcgacaa gcagatcaag     4560

gagctcctgg acctcaaggt catcaagccc tccaagagcc cccacatggc tcccgcgttc     4620

ctggtcaaca acgaggcgga gaacggccgc ggcaacaaga ggatggtggt caactacaag     4680

gccatgaaca aggccacggt gggcgacgcc tacaacctcc ctaacaagga cgagctcctg     4740

acgctgatcc gcggcaagaa gatcttctcc agcttcgact gcaagtccgg cttctggcag     4800

gtcctgctcg accaggagag caggcccctc acggccttca cgtgccccca gggccactac     4860

gagtggaacg tggtcccatt cggcctgaag caggccccca gcatcttcca gcgccacatg     4920

gacgaggcct tccgcgtgtt caggaagttc tgctgcgtct acgtggacga catcgtggtc     4980

ttctccaaca acgaggagga ccacctcctg cacgtcgcga tgatcctcca gaagtgcaac     5040

cagcacggca tcatcctctc caagaagaag gcccagctgt tcaagaagaa gatcaacttc     5100

ctcggcctcg agatcgacga gggcacccac aagccacagg gccacatcct cgagcacatc     5160

aacaagttcc cggacaccct cgaggacaag aagcagctgc agcgcttcct cggcatcctg     5220

acgtacgcct ccgactacat ccccaacctg gcccagatga ggcagcccct ccaggccaag     5280

ctgaaggaga acgtgccctg gaagtggacc aaggaggaca cgctctacat gcagaaggtc     5340

aagaagaacc tgcagggctt cccgcccctc caccaccccc tgcccgagga gaagctcatc     5400

atcgagacgg acgcgagcga cgactactgg ggcggcatgc tcaaggccat caagatcaac     5460

gagggcacca acacggagct gatctgccgc taccgctcgg gcagcttcaa ggcggccgag     5520

cgcaactacc actccaacga caaggagacg ctggccgtga tcaacacgat caagaagttc     5580

agcatctacc tcaccccggt ccacttcctg atcaggaccg acaacacgca cttcaagtcc     5640

ttcgtcaacc tcaactacaa gggcgacagc aagctgggcc gcaacatcag gtggcaggcg     5700

tggctctccc actacagctt cgacgtggag cacatcaagg gcacggacaa ccacttcgcc     5760

gacttcctgt ccagggagtt caacaaggtc aacagctccg gcggcagcaa gcgcacggcg     5820

gacggctcgg agttcgagcc gaagaagaag cgcaaggtgt ga                        5862


<210>  69
<211>  7224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-KarmaRT

<400>  69
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcccgctc     4260

ctgctcaccg ccagcacgtc catccccaag agcaagacct tcaggttcga gaaccacctg     4320

ctcctggacc gcgacttcct gccgtccgtc atgcacaggt ggccccagca gctcaacatg     4380

aacgaccccg cggcctgcct ggcggccaag ctgaagcaga cccgcagcat catcaaggtc     4440

tggatgaagg agcacaggaa gcgctccagc ctgaacgagg actgcaagtt cacgatcgac     4500

ctcctggact acatcgagga gctcaggccc ctgaacgacg gcgagaggtg cctccgcaac     4560

ctggtgcagg agaagctgtt ccaggccatc cagtccaagg tggcgtactg gaagcagagg     4620

ggcaaggtca agcgcctcaa gatcggcatc gacaacagcg ccttcttcaa ggcccacgcc     4680

tccaagaact accgcaacag caggatccgc tccatcaagc acaacggcgc cgagatcgcg     4740

gaccacaacg gcaaggcgct cctgctccac agcttcttcc agcacttcct gggctcggac     4800

tgccagcccc tctgggactt cgacctcagc accctgtact ccgaggacga gagggactac     4860

aacctgctcg ccagccaggg cctccagttc tccaccgagg agatcatcac gagcatcaag     4920

ggcatgaaca gcaactcggc tcccggcccg gacggcttcg gcccgagctt ctacaactcc     4980

gcgtggaaca gcatcaagga ggacatcatc aagctggccg cgtccttctg cagcaactcc     5040

gtgcagctgg agaggatcaa ccgcgcgcac atcgtcctca tcccgaagcc cggcaaggag     5100

aacacggtgg acggctacag gcccatctcc ctccagaact gcagcgtgaa gatcctctcc     5160

aaggtcctgg ccaacaggct ccagaaggtc ctgacccgca tgatccacct ggaccagacg     5220

ggcttcctca agggccgctg catcagcgag aacctgatct acgccacgga gctcatccag     5280

gcttgccacg cgaggaagtg caagacgctc atcatcaagc tggacttcgc caaggcgttc     5340

gacagcgtcc tctggacctc cctgttccag atcctcgccg tgcgcggctt cccgaacaac     5400

tggatctcct ggatcaagag cctgctccag acgagcaagt ccgccgtgct gctcaacggc     5460

atcccgggca agtggatcaa ctgcaagaag ggcctgaggc agggcgaccc gctctccccc     5520

tacctcttca tcctggtggc cgacgtcctg cagaggctgc tcgagaagaa cttccagatc     5580

cggcacccca tctaccagga caggccatgc gctaccatcc agtacgccga cgacaccctc     5640

gtgatctgca gggcggagga ggacgacgtc ctggcgctga ggagcaccct gctccagttc     5700

tccaaggcca cgggcctcca gatcaacttc gcgaagtcca ccatgatcag cctgcacatc     5760

gaccgcagca aggagtccag cctctccgag ctgctccagt gcaagctcga gtcgctcccc     5820

atgagctacc tcggcctgcc actcagcctg cacaagctga ccaacaacga cctccagccg     5880

atcgtggtca aggtcgactc cttcctgacc ggctgggagg ctagcctgct ctcccaggcg     5940

gagaggctca tcctggtgaa cgcggtcctg tcctcggtgc cggtctacgc catgagcgcg     6000

ttcaagctcc cgcccaaggt catcgaggcc atcgacaaga ggaggcgcgc gttcttctgg     6060

acgggcgacg acacctgctc gggcgctaag tgcctggtgg cctgggacga ggtgtgcacg     6120

gctaaggaga agggcggcct gggcatcaag agcctcaaga cgcagaacga ggcgctgctc     6180

ctgaagcgcc tcttcaacct gttctccgac aactccagct ggaccaactg gatctggaag     6240

gagttcgacg gcaggtccct cctcaagtcc ctcccgctgg gccagcactg gagcgtcttc     6300

cagaagctcc tgcccgagct gttcaagatc accacggtgc acatcggcga cggcagcagg     6360

acgtccttct ggcacgacag gtggacgggc aacatgaccc tggctgcgca gttcgagagc     6420

ctcttcagcc actccacgga cgacctggct accgtggagc agatcctctc cttcggcatc     6480

tacgacatcc tcaccccgcg cctctcgtcg gcggcccaga acgacctcca ggagctgcag     6540

acgatcctgc agaacttcag cctcaccaac gagtccgaca cgaggctgaa caaccgcctc     6600

ggcaacctga ccacgaagac gatctacgac ctccgcagcc cgcccggctt cccctccccc     6660

aactggaagt tcatctggga ctgcaggatg ccgctgaaga tcaagctgtt cgcttggctc     6720

ctggtcaggg accgcctgag caccaagctc aacctcctca agaagaagat cgtgcagacc     6780

gccacgtgcg acatctgcag caccaccgac gagacggccg accacctgag cttcaactgc     6840

ccgttcgcca tctccttctg gcaggcgctc cacatccagc cgatcatcaa cgagacgaag     6900

tacctcagcc agctcaaggc tcccgccacc atcccaacgc gccacttcca gtccttcttc     6960

atgctgtgct tctgggtcct ctggaaccac aggcacgacg tggtcttcag gggccgcccg     7020

cccagcatcg cttcctgcct ccagaggggc atctcggagt cgtccctgtg ggccgagacg     7080

ttcacccccg acgacaggtt cgtgatcgac gtctggaagg gcgtgttctc cagctccctc     7140

cagacgctgc accccatgtc cggcggcagc aagcgcacgg cggacggctc ggagttcgag     7200

ccgaagaaga agcgcaaggt gtga                                            7224


<210>  70
<211>  6171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1RT

<400>  70
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcgccgcg     4260

gacatgtccg agaaggtgaa gaacggcatc atcccgaact tcgtcaccat cccctccacg     4320

agcaacaacc ccacctcggc cgagtcgacg accgacgagg tgagcgagca gggcgagcag     4380

ccaggcgagg tcatcgagca gggcgagcag ctggacgagg gcgtggagga ggtggagcac     4440

ccgacccagg gcgaggagca gcaccagcca ctgaggaggt cggagcgccc gagggtggag     4500

tccaggaggt accccagcac cgagtatgtg ctcatctcgg acgacaggga gccggagtcc     4560

ctgaaggagg tgctcagcca cccggagaag aaccagctca tgaaggcgat gcaggaggag     4620

atggagtccc tgcagaagaa cggcacctac aagctggtcg agctcccgaa gggcaagagg     4680

cccctcaagt gcaagtgggt gttcaagctg aagaaggacg gcgactgcaa gctcgtccgc     4740

tacaaggcca ggctggtggt caagggcttc gagcagaaga agggcatcga cttcgacgag     4800

atcttctccc ccgtggtcaa gatgaccagc atcaggacca tcctgtcgct ggcggccagc     4860

ctggacctcg aggtggagca gctggacgtc aagaccgcct tcctgcacgg cgacctcgag     4920

gaggagatct acatggagca gcccgagggc ttcgaggtgg ccggcaagaa gcacatggtc     4980

tgcaagctga acaagtcgct ctacggcctc aagcaggccc cgaggcagtg gtacatgaag     5040

ttcgactcct tcatgaagtc ccagacctac ctcaagacgt actccgaccc gtgcgtgtac     5100

ttcaagaggt tcagcgagaa caacttcatc atcctcctgc tctacgtgga cgacatgctc     5160

atcgtcggca aggacaaggg cctgatcgcg aagctcaagg gcgacctgtc caagagcttc     5220

gacatgaagg acctcggccc ggctcagcag atcctgggca tgaagatcgt gcgcgagagg     5280

acctccagga agctgtggct cagccaggag aagtacatcg agcgcgtgct cgagaggttc     5340

aacatgaaga acgccaagcc ggtgtccacg cccctggctg gccacctgaa gctcagcaag     5400

aagatgtgcc ccaccacggt cgaggagaag ggcaacatgg ctaaggtgcc ctactcctcg     5460

gccgtgggct ccctgatgta cgctatggtg tgcacccgcc ccgacatcgc tcacgccgtg     5520

ggcgtggtca gcaggttcct cgagaacccg ggcaaggagc actgggaggc ggtgaagtgg     5580

atcctcaggt acctccgggg caccaccggc gactgcctct gcttcggtgg ctccgacccg     5640

atcctgaagg gctacaccga cgcggacatg gccggcgaca tcgacaaccg caagtccagc     5700

accggctacc tgttcacgtt ctccggcggc gccatctcct ggcagagcaa gctccagaag     5760

tgcgtggctc tgagcacgac cgaggccgag tacatcgcgg ccacggagac gggcaaggag     5820

atgatctggc tcaagcgctt cctgcaggag ctgggcctcc accagaagga gtatgtggtc     5880

tactgcgact cccagagcgc gatcgacctg tccaagaaca gcatgtacca cgccaggacc     5940

aagcacatcg acgtgcgcta ccactggatc agggagatgg tggacgacga gtccctgaag     6000

gtcctcaaga tcagcaccaa cgagaacccc gccgacatgc tcacgaaggt ggtcccgagg     6060

aacaagttcg agctctgcaa ggagctggtc ggcatgcaca gcaactccgg cggcagcaag     6120

cgcacggcgg acggctcgga gttcgagccg aagaagaagc gcaaggtgtg a              6171


<210>  71
<211>  21
<212>  DNA
<213>  Physcomitrella patens

<400>  71
gatgtcttag gccctgtgat t                                                 21


<210>  72
<211>  443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-pegRNA-APT#1

<400>  72
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccatgat gtcttaggcc ctgtgattgt tttagagcta gaaatagcaa gttaaaataa      360

ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctactac actcttagct      420

aatcacaggg cctaagtttt ttt                                              443


<210>  73
<211>  20
<212>  DNA
<213>  Physcomitrella patens

<400>  73
ggtgaagatg tcggcctcca                                                   20


<210>  74
<211>  442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-pegRNA-APT#2

<400>  74
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccatggt gaagatgtcg gcctccagtt ttagagctag aaatagcaag ttaaaataag      360

gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcgtcgcca tccttggtat      420

gaggccgaca tcttcttttt tt                                               442


<210>  75
<211>  20
<212>  DNA
<213>  Physcomitrella patens

<400>  75
tgtcttaggc cctgtgatta                                                   20


<210>  76
<211>  449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-pegRNA-APT#3

<400>  76
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccattgt cttaggccct gtgattagtt taagagctat gctggaaaca gcatagcaag      360

tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctatactc      420

ttctcaaatc acagggccta agttttttt                                        449


<210>  77
<211>  20
<212>  DNA
<213>  Physcomitrella patens

<400>  77
gatgacttaa ttgccaccgg                                                   20


<210>  78
<211>  449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-pegRNA-APT#6

<400>  78
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccatgat gacttaattg ccaccgggtt taagagctat gctggaaaca gcatagcaag      360

tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctccaaga      420

gttcatcagg tggcaattaa gtttttttt                                        449


<210>  79
<211>  307
<212>  DNA
<213>  Physcomitrella patens

<400>  79
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtgcagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccat                                                                307


<210>  80
<211>  20
<212>  DNA
<213>  Physcomitrella patens

<400>  80
acgtcagcac aaaattgtca                                                   20


<210>  81
<211>  482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-gRNA-APT#3

<400>  81
tctatattag atgattgttc atgtagaaca caaggaagaa atacaaaacg tgtccatgca       60

cataagatct agagtattgg ataaaataat acgtatagat gtatagatga gcaagtagta      120

aaagatgact tactcctagt taaattaagc atggaaagaa agtgttagca tgtacaaaag      180

agaaataaaa ataaaaacac aaaacctctg tatcgataga tttcttagaa ggggaacgaa      240

acataaaact tcaagtgaat gcaagtgatg gatggatact attgagtgag tgcggggcag      300

gggaggtgga gttgcaccat agccgttgcg gtggaagggg aaggccggta tatggaggtg      360

ggggtggagc cggtctggca cgtcagcaca aaattgtcag ttttagagct agaaatagca      420

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      480

tt                                                                     482


<210>  82
<211>  315
<212>  DNA
<213>  Physcomitrella patens

<400>  82
agatctagag tattggataa aataatacgt atagatgtat agatgagcaa gtagtaaaag       60

atgacttact cctagttaaa ttaagcatgg aaagaaagtg ttagcatgta caaaagagaa      120

ataaaaataa aaacacaaaa cctctgtatc gatagatttc ttagaagggg aacgaaacat      180

aaaacttcaa gtgaatgcaa gtgatggatg gatactattg agtgagtgcg gggcagggga      240

ggtggagttg caccatagcc gttgcggtgg aaggggaagg ccggtatatg gaggtggggg      300

tggagccggt ctggc                                                       315


<210>  83
<211>  20
<212>  DNA
<213>  Physcomitrella patens

<400>  83
ggatgctgtc cgagatatac                                                   20


<210>  84
<211>  410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEntPp-gRNA-APT#5

<400>  84
aagcagacgt gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt       60

gtggttcttc cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg      120

cagcgggaga gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg      180

ccgagtggac gcgaccgggt ttcgaggggg ggggggagaa aagggatgga gcgagggata      240

taacccacat ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac      300

aacccatgga tgctgtccga gatatacgtt ttagagctag aaatagcaag ttaaaataag      360

gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt                 410


<210>  85
<211>  464
<212>  PRT
<213>  Nicotiana tabacum

<400>  85

Ala Ala Asp Met Ser Glu Lys Val Lys Asn Gly Ile Ile Pro Asn Phe 
1               5                   10                  15      


Val Thr Ile Pro Ser Thr Ser Asn Asn Pro Thr Ser Ala Glu Ser Thr 
            20                  25                  30          


Thr Asp Glu Val Ser Glu Gln Gly Glu Gln Pro Gly Glu Val Ile Glu 
        35                  40                  45              


Gln Gly Glu Gln Leu Asp Glu Gly Val Glu Glu Val Glu His Pro Thr 
    50                  55                  60                  


Gln Gly Glu Glu Gln His Gln Pro Leu Arg Arg Ser Glu Arg Pro Arg 
65                  70                  75                  80  


Val Glu Ser Arg Arg Tyr Pro Ser Thr Glu Tyr Val Leu Ile Ser Asp 
                85                  90                  95      


Asp Arg Glu Pro Glu Ser Leu Lys Glu Val Leu Ser His Pro Glu Lys 
            100                 105                 110         


Asn Gln Leu Met Lys Ala Met Gln Glu Glu Met Glu Ser Leu Gln Lys 
        115                 120                 125             


Asn Gly Thr Tyr Lys Leu Val Glu Leu Pro Lys Gly Lys Arg Pro Leu 
    130                 135                 140                 


Lys Cys Lys Trp Val Phe Lys Leu Lys Lys Asp Gly Asp Cys Lys Leu 
145                 150                 155                 160 


Val Arg Tyr Lys Ala Arg Leu Val Val Lys Gly Phe Glu Gln Lys Lys 
                165                 170                 175     


Gly Ile Asp Phe Asp Glu Ile Phe Ser Pro Val Val Lys Met Thr Ser 
            180                 185                 190         


Ile Arg Thr Ile Leu Ser Leu Ala Ala Ser Leu Asp Leu Glu Val Glu 
        195                 200                 205             


Gln Leu Asp Val Lys Thr Ala Phe Leu His Gly Asp Leu Glu Glu Glu 
    210                 215                 220                 


Ile Tyr Met Glu Gln Pro Glu Gly Phe Glu Val Ala Gly Lys Lys His 
225                 230                 235                 240 


Met Val Cys Lys Leu Asn Lys Ser Leu Tyr Gly Leu Lys Gln Ala Pro 
                245                 250                 255     


Arg Gln Trp Tyr Met Lys Phe Asp Ser Phe Met Lys Ser Gln Thr Tyr 
            260                 265                 270         


Leu Lys Thr Tyr Ser Asp Pro Cys Val Tyr Phe Lys Arg Phe Ser Glu 
        275                 280                 285             


Asn Asn Phe Ile Ile Leu Leu Leu Tyr Val Asp Asp Met Leu Ile Val 
    290                 295                 300                 


Gly Lys Asp Lys Gly Leu Ile Ala Lys Leu Lys Gly Asp Leu Ser Lys 
305                 310                 315                 320 


Ser Phe Asp Met Lys Asp Leu Gly Pro Ala Gln Gln Ile Leu Gly Met 
                325                 330                 335     


Lys Ile Val Arg Glu Arg Thr Ser Arg Lys Leu Trp Leu Ser Gln Glu 
            340                 345                 350         


Lys Tyr Ile Glu Arg Val Leu Glu Arg Phe Asn Met Lys Asn Ala Lys 
        355                 360                 365             


Pro Val Ser Thr Pro Leu Ala Gly His Leu Lys Leu Ser Lys Lys Met 
    370                 375                 380                 


Cys Pro Thr Thr Val Glu Glu Lys Gly Asn Met Ala Lys Val Pro Tyr 
385                 390                 395                 400 


Ser Ser Ala Val Gly Ser Leu Met Tyr Ala Met Val Cys Thr Arg Pro 
                405                 410                 415     


Asp Ile Ala His Ala Val Gly Val Val Ser Arg Phe Leu Glu Asn Pro 
            420                 425                 430         


Gly Lys Glu His Trp Glu Ala Val Lys Trp Ile Leu Arg Tyr Leu Arg 
        435                 440                 445             


Gly Thr Thr Gly Asp Cys Leu Cys Phe Gly Gly Ser Asp Pro Ile Leu 
    450                 455                 460                 


<210>  86
<211>  5712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1RTv2

<400>  86
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcgccgcg     4260

gacatgtccg agaaggtgaa gaacggcatc atcccgaact tcgtcaccat cccctccacg     4320

agcaacaacc ccacctcggc cgagtcgacg accgacgagg tgagcgagca gggcgagcag     4380

ccaggcgagg tcatcgagca gggcgagcag ctggacgagg gcgtggagga ggtggagcac     4440

ccgacccagg gcgaggagca gcaccagcca ctgaggaggt cggagcgccc gagggtggag     4500

tccaggaggt accccagcac cgagtatgtg ctcatctcgg acgacaggga gccggagtcc     4560

ctgaaggagg tgctcagcca cccggagaag aaccagctca tgaaggcgat gcaggaggag     4620

atggagtccc tgcagaagaa cggcacctac aagctggtcg agctcccgaa gggcaagagg     4680

cccctcaagt gcaagtgggt gttcaagctg aagaaggacg gcgactgcaa gctcgtccgc     4740

tacaaggcca ggctggtggt caagggcttc gagcagaaga agggcatcga cttcgacgag     4800

atcttctccc ccgtggtcaa gatgaccagc atcaggacca tcctgtcgct ggcggccagc     4860

ctggacctcg aggtggagca gctggacgtc aagaccgcct tcctgcacgg cgacctcgag     4920

gaggagatct acatggagca gcccgagggc ttcgaggtgg ccggcaagaa gcacatggtc     4980

tgcaagctga acaagtcgct ctacggcctc aagcaggccc cgaggcagtg gtacatgaag     5040

ttcgactcct tcatgaagtc ccagacctac ctcaagacgt actccgaccc gtgcgtgtac     5100

ttcaagaggt tcagcgagaa caacttcatc atcctcctgc tctacgtgga cgacatgctc     5160

atcgtcggca aggacaaggg cctgatcgcg aagctcaagg gcgacctgtc caagagcttc     5220

gacatgaagg acctcggccc ggctcagcag atcctgggca tgaagatcgt gcgcgagagg     5280

acctccagga agctgtggct cagccaggag aagtacatcg agcgcgtgct cgagaggttc     5340

aacatgaaga acgccaagcc ggtgtccacg cccctggctg gccacctgaa gctcagcaag     5400

aagatgtgcc ccaccacggt cgaggagaag ggcaacatgg ctaaggtgcc ctactcctcg     5460

gccgtgggct ccctgatgta cgctatggtg tgcacccgcc ccgacatcgc tcacgccgtg     5520

ggcgtggtca gcaggttcct cgagaacccg ggcaaggagc actgggaggc ggtgaagtgg     5580

atcctcaggt acctccgggg caccaccggc gactgcctct gcttcggtgg ctccgacccg     5640

atcctgtccg gcggcagcaa gcgcacggcg gacggctcgg agttcgagcc gaagaagaag     5700

cgcaaggtgt ga                                                         5712


<210>  87
<211>  617
<212>  PRT
<213>  Nicotiana tabacum

<400>  87

Ala Ala Asp Met Ser Glu Lys Val Lys Asn Gly Ile Ile Pro Asn Phe 
1               5                   10                  15      


Val Thr Ile Pro Ser Thr Ser Asn Asn Pro Thr Ser Ala Glu Ser Thr 
            20                  25                  30          


Thr Asp Glu Val Ser Glu Gln Gly Glu Gln Pro Gly Glu Val Ile Glu 
        35                  40                  45              


Gln Gly Glu Gln Leu Asp Glu Gly Val Glu Glu Val Glu His Pro Thr 
    50                  55                  60                  


Gln Gly Glu Glu Gln His Gln Pro Leu Arg Arg Ser Glu Arg Pro Arg 
65                  70                  75                  80  


Val Glu Ser Arg Arg Tyr Pro Ser Thr Glu Tyr Val Leu Ile Ser Asp 
                85                  90                  95      


Asp Arg Glu Pro Glu Ser Leu Lys Glu Val Leu Ser His Pro Glu Lys 
            100                 105                 110         


Asn Gln Leu Met Lys Ala Met Gln Glu Glu Met Glu Ser Leu Gln Lys 
        115                 120                 125             


Asn Gly Thr Tyr Lys Leu Val Glu Leu Pro Lys Gly Lys Arg Pro Leu 
    130                 135                 140                 


Lys Cys Lys Trp Val Phe Lys Leu Lys Lys Asp Gly Asp Cys Lys Leu 
145                 150                 155                 160 


Val Arg Tyr Lys Ala Arg Leu Val Val Lys Gly Phe Glu Gln Lys Lys 
                165                 170                 175     


Gly Ile Asp Phe Asp Glu Ile Phe Ser Pro Val Val Lys Met Thr Ser 
            180                 185                 190         


Ile Arg Thr Ile Leu Ser Leu Ala Ala Ser Leu Asp Leu Glu Val Glu 
        195                 200                 205             


Gln Leu Asp Val Lys Thr Ala Phe Leu His Gly Asp Leu Glu Glu Glu 
    210                 215                 220                 


Ile Tyr Met Glu Gln Pro Glu Gly Phe Glu Val Ala Gly Lys Lys His 
225                 230                 235                 240 


Met Val Cys Lys Leu Asn Lys Ser Leu Tyr Gly Leu Lys Gln Ala Pro 
                245                 250                 255     


Arg Gln Trp Tyr Met Lys Phe Asp Ser Phe Met Lys Ser Gln Thr Tyr 
            260                 265                 270         


Leu Lys Thr Tyr Ser Asp Pro Cys Val Tyr Phe Lys Arg Phe Ser Glu 
        275                 280                 285             


Asn Asn Phe Ile Ile Leu Leu Leu Tyr Val Asp Asp Met Leu Ile Val 
    290                 295                 300                 


Gly Lys Asp Lys Gly Leu Ile Ala Lys Leu Lys Gly Asp Leu Ser Lys 
305                 310                 315                 320 


Ser Phe Asp Met Lys Asp Leu Gly Pro Ala Gln Gln Ile Leu Gly Met 
                325                 330                 335     


Lys Ile Val Arg Glu Arg Thr Ser Arg Lys Leu Trp Leu Ser Gln Glu 
            340                 345                 350         


Lys Tyr Ile Glu Arg Val Leu Glu Arg Phe Asn Met Lys Asn Ala Lys 
        355                 360                 365             


Pro Val Ser Thr Pro Leu Ala Gly His Leu Lys Leu Ser Lys Lys Met 
    370                 375                 380                 


Cys Pro Thr Thr Val Glu Glu Lys Gly Asn Met Ala Lys Val Pro Tyr 
385                 390                 395                 400 


Ser Ser Ala Val Gly Ser Leu Met Tyr Ala Met Val Cys Thr Arg Pro 
                405                 410                 415     


Asp Ile Ala His Ala Val Gly Val Val Ser Arg Phe Leu Glu Asn Pro 
            420                 425                 430         


Gly Lys Glu His Trp Glu Ala Val Lys Trp Ile Leu Arg Tyr Leu Arg 
        435                 440                 445             


Gly Thr Thr Gly Asp Cys Leu Cys Phe Gly Gly Ser Asp Pro Ile Leu 
    450                 455                 460                 


Lys Gly Tyr Thr Asn Ala Asp Met Ala Gly Asp Ile Asp Asn Arg Lys 
465                 470                 475                 480 


Ser Ser Thr Gly Tyr Leu Phe Thr Phe Ser Gly Gly Ala Ile Ser Trp 
                485                 490                 495     


Gln Ser Lys Leu Gln Lys Cys Val Ala Leu Ser Thr Thr Glu Ala Glu 
            500                 505                 510         


Tyr Ile Ala Ala Thr Glu Thr Gly Lys Glu Met Ile Trp Leu Lys Arg 
        515                 520                 525             


Phe Leu Gln Glu Leu Gly Leu His Gln Lys Glu Tyr Val Val Tyr Cys 
    530                 535                 540                 


Asp Ser Gln Ser Ala Ile Asp Leu Ser Lys Asn Ser Met Tyr His Ala 
545                 550                 555                 560 


Arg Thr Lys His Ile Asp Val Arg Tyr His Trp Ile Arg Glu Met Val 
                565                 570                 575     


Asp Asp Glu Ser Leu Lys Val Leu Lys Ile Ser Thr Asn Glu Asn Pro 
            580                 585                 590         


Ala Asp Met Leu Thr Lys Val Val Pro Arg Asn Lys Phe Glu Leu Cys 
        595                 600                 605             


Lys Glu Leu Val Gly Met His Ser Asn 
    610                 615         


<210>  88
<211>  6171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1(D469N)RT

<400>  88
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcgccgcg     4260

gacatgtccg agaaggtgaa gaacggcatc atcccgaact tcgtcaccat cccctccacg     4320

agcaacaacc ccacctcggc cgagtcgacg accgacgagg tgagcgagca gggcgagcag     4380

ccaggcgagg tcatcgagca gggcgagcag ctggacgagg gcgtggagga ggtggagcac     4440

ccgacccagg gcgaggagca gcaccagcca ctgaggaggt cggagcgccc gagggtggag     4500

tccaggaggt accccagcac cgagtatgtg ctcatctcgg acgacaggga gccggagtcc     4560

ctgaaggagg tgctcagcca cccggagaag aaccagctca tgaaggcgat gcaggaggag     4620

atggagtccc tgcagaagaa cggcacctac aagctggtcg agctcccgaa gggcaagagg     4680

cccctcaagt gcaagtgggt gttcaagctg aagaaggacg gcgactgcaa gctcgtccgc     4740

tacaaggcca ggctggtggt caagggcttc gagcagaaga agggcatcga cttcgacgag     4800

atcttctccc ccgtggtcaa gatgaccagc atcaggacca tcctgtcgct ggcggccagc     4860

ctggacctcg aggtggagca gctggacgtc aagaccgcct tcctgcacgg cgacctcgag     4920

gaggagatct acatggagca gcccgagggc ttcgaggtgg ccggcaagaa gcacatggtc     4980

tgcaagctga acaagtcgct ctacggcctc aagcaggccc cgaggcagtg gtacatgaag     5040

ttcgactcct tcatgaagtc ccagacctac ctcaagacgt actccgaccc gtgcgtgtac     5100

ttcaagaggt tcagcgagaa caacttcatc atcctcctgc tctacgtgga cgacatgctc     5160

atcgtcggca aggacaaggg cctgatcgcg aagctcaagg gcgacctgtc caagagcttc     5220

gacatgaagg acctcggccc ggctcagcag atcctgggca tgaagatcgt gcgcgagagg     5280

acctccagga agctgtggct cagccaggag aagtacatcg agcgcgtgct cgagaggttc     5340

aacatgaaga acgccaagcc ggtgtccacg cccctggctg gccacctgaa gctcagcaag     5400

aagatgtgcc ccaccacggt cgaggagaag ggcaacatgg ctaaggtgcc ctactcctcg     5460

gccgtgggct ccctgatgta cgctatggtg tgcacccgcc ccgacatcgc tcacgccgtg     5520

ggcgtggtca gcaggttcct cgagaacccg ggcaaggagc actgggaggc ggtgaagtgg     5580

atcctcaggt acctccgggg caccaccggc gactgcctct gcttcggtgg ctccgacccg     5640

atcctgaagg gctacaccaa cgcggacatg gccggcgaca tcgacaaccg caagtccagc     5700

accggctacc tgttcacgtt ctccggcggc gccatctcct ggcagagcaa gctccagaag     5760

tgcgtggctc tgagcacgac cgaggccgag tacatcgcgg ccacggagac gggcaaggag     5820

atgatctggc tcaagcgctt cctgcaggag ctgggcctcc accagaagga gtatgtggtc     5880

tactgcgact cccagagcgc gatcgacctg tccaagaaca gcatgtacca cgccaggacc     5940

aagcacatcg acgtgcgcta ccactggatc agggagatgg tggacgacga gtccctgaag     6000

gtcctcaaga tcagcaccaa cgagaacccc gccgacatgc tcacgaaggt ggtcccgagg     6060

aacaagttcg agctctgcaa ggagctggtc ggcatgcaca gcaactccgg cggcagcaag     6120

cgcacggcgg acggctcgga gttcgagccg aagaagaagc gcaaggtgtg a              6171


<210>  89
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BMPmm

<400>  89
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

cttttgacca ccttcaccca cggcgtgcag tgcttcggcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcagc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa acagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  90
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BMPmm

<400>  90

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Leu Thr Thr 
    50                  55                  60                  


Phe Thr His Gly Val Gln Cys Phe Gly Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  91
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFPmm

<400>  91
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

cttttgacca ccttcaccta cggcgtgcag tgcttcggcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcagc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa acagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  92
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFPmm

<400>  92

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Leu Thr Thr 
    50                  55                  60                  


Phe Thr Tyr Gly Val Gln Cys Phe Gly Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pegRNA-BFP-01 Target

<400>  93
cttttgacca ccttcaccca                                                   20


<210>  94
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pegRNA-BFP-01

<400>  94
cttttgacca ccttcaccca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcactg cacgccgtag gtgaaggtgg      120

tca                                                                    123


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-BFP-R3 Target

<400>  95
ggtggtgcag atgaacttca                                                   20


<210>  96
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-BFP-R3

<400>  96
ggtggtgcag atgaacttca gtttaagagc tagaaatagc aagtttaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgc                                 96


<210>  97
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-BFP-PE3b Target

<400>  97
cactgcacgc cgtaggtga                                                    19


<210>  98
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-PE3b-01

<400>  98
cactgcacgc cgtaggtgag ttttagagct agaaatagca agttaaaata aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgc                                  95


<210>  99
<211>  238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BFP_SpCas9_pegRNA_RZ_01

<400>  99
caaaagctga tgagtccgtg aggacgaaac gagtaagctc gtccttttga ccaccttcac       60

ccagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa      120

agtggcaccg agtcggtgca ctgcacgccg taggtgaagg tggtcatttt ggccggcatg      180

gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg aatgggac        238


<210>  100
<211>  211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BFP_SpCas9_gRNA_RZ_R3

<400>  100
accaccctga tgagtccgtg aggacgaaac gagtaagctc gtcggtggtg cagatgaact       60

tcagtttaag agctagaaat agcaagttta aataaggcta gtccgttatc aacttgaaaa      120

agtggcaccg agtcggtgct tttggccggc atggtcccag cctcctcgct ggcgccggct      180

gggcaacatg cttcggcatg gcgaatggga c                                     211


<210>  101
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BFP_SpCas9_gRNA_RZ_PE3b

<400>  101
gcagtgctga tgagtccgtg aggacgaaac gagtaagctc gtccactgca cgccgtaggt       60

gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa      120

gtggcaccga gtcggtgctt ttggccggca tggtcccagc ctcctcgctg gcgccggctg      180

ggcaacatgc ttcggcatgg cgaatgggac                                       210


<210>  102
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 NLS

<400>  102
atggctccta agaagaagcg caaggttggg atccacgggg tt                          42


<210>  103
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XlNucleoplasmin NLS

<400>  103
aagaggccgg cggcaaccaa gaaggcgggc caggccaaga agaagaag                    48


<210>  104
<211>  1903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nCas9-Tnt1_RTv2

<400>  104

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Ser Gly Gly Ser Ser  Gly Gly Ser Ser Gly  Ser Glu Thr 
    1385                 1390                 1395             


Pro Gly  Thr Ser Glu Ser Ala  Thr Pro Glu Ser Ser  Gly Gly Ser 
    1400                 1405                 1410             


Ser Gly  Gly Ser Ser Ala Ala  Asp Met Ser Glu Lys  Val Lys Asn 
    1415                 1420                 1425             


Gly Ile  Ile Pro Asn Phe Val  Thr Ile Pro Ser Thr  Ser Asn Asn 
    1430                 1435                 1440             


Pro Thr  Ser Ala Glu Ser Thr  Thr Asp Glu Val Ser  Glu Gln Gly 
    1445                 1450                 1455             


Glu Gln  Pro Gly Glu Val Ile  Glu Gln Gly Glu Gln  Leu Asp Glu 
    1460                 1465                 1470             


Gly Val  Glu Glu Val Glu His  Pro Thr Gln Gly Glu  Glu Gln His 
    1475                 1480                 1485             


Gln Pro  Leu Arg Arg Ser Glu  Arg Pro Arg Val Glu  Ser Arg Arg 
    1490                 1495                 1500             


Tyr Pro  Ser Thr Glu Tyr Val  Leu Ile Ser Asp Asp  Arg Glu Pro 
    1505                 1510                 1515             


Glu Ser  Leu Lys Glu Val Leu  Ser His Pro Glu Lys  Asn Gln Leu 
    1520                 1525                 1530             


Met Lys  Ala Met Gln Glu Glu  Met Glu Ser Leu Gln  Lys Asn Gly 
    1535                 1540                 1545             


Thr Tyr  Lys Leu Val Glu Leu  Pro Lys Gly Lys Arg  Pro Leu Lys 
    1550                 1555                 1560             


Cys Lys  Trp Val Phe Lys Leu  Lys Lys Asp Gly Asp  Cys Lys Leu 
    1565                 1570                 1575             


Val Arg  Tyr Lys Ala Arg Leu  Val Val Lys Gly Phe  Glu Gln Lys 
    1580                 1585                 1590             


Lys Gly  Ile Asp Phe Asp Glu  Ile Phe Ser Pro Val  Val Lys Met 
    1595                 1600                 1605             


Thr Ser  Ile Arg Thr Ile Leu  Ser Leu Ala Ala Ser  Leu Asp Leu 
    1610                 1615                 1620             


Glu Val  Glu Gln Leu Asp Val  Lys Thr Ala Phe Leu  His Gly Asp 
    1625                 1630                 1635             


Leu Glu  Glu Glu Ile Tyr Met  Glu Gln Pro Glu Gly  Phe Glu Val 
    1640                 1645                 1650             


Ala Gly  Lys Lys His Met Val  Cys Lys Leu Asn Lys  Ser Leu Tyr 
    1655                 1660                 1665             


Gly Leu  Lys Gln Ala Pro Arg  Gln Trp Tyr Met Lys  Phe Asp Ser 
    1670                 1675                 1680             


Phe Met  Lys Ser Gln Thr Tyr  Leu Lys Thr Tyr Ser  Asp Pro Cys 
    1685                 1690                 1695             


Val Tyr  Phe Lys Arg Phe Ser  Glu Asn Asn Phe Ile  Ile Leu Leu 
    1700                 1705                 1710             


Leu Tyr  Val Asp Asp Met Leu  Ile Val Gly Lys Asp  Lys Gly Leu 
    1715                 1720                 1725             


Ile Ala  Lys Leu Lys Gly Asp  Leu Ser Lys Ser Phe  Asp Met Lys 
    1730                 1735                 1740             


Asp Leu  Gly Pro Ala Gln Gln  Ile Leu Gly Met Lys  Ile Val Arg 
    1745                 1750                 1755             


Glu Arg  Thr Ser Arg Lys Leu  Trp Leu Ser Gln Glu  Lys Tyr Ile 
    1760                 1765                 1770             


Glu Arg  Val Leu Glu Arg Phe  Asn Met Lys Asn Ala  Lys Pro Val 
    1775                 1780                 1785             


Ser Thr  Pro Leu Ala Gly His  Leu Lys Leu Ser Lys  Lys Met Cys 
    1790                 1795                 1800             


Pro Thr  Thr Val Glu Glu Lys  Gly Asn Met Ala Lys  Val Pro Tyr 
    1805                 1810                 1815             


Ser Ser  Ala Val Gly Ser Leu  Met Tyr Ala Met Val  Cys Thr Arg 
    1820                 1825                 1830             


Pro Asp  Ile Ala His Ala Val  Gly Val Val Ser Arg  Phe Leu Glu 
    1835                 1840                 1845             


Asn Pro  Gly Lys Glu His Trp  Glu Ala Val Lys Trp  Ile Leu Arg 
    1850                 1855                 1860             


Tyr Leu  Arg Gly Thr Thr Gly  Asp Cys Leu Cys Phe  Gly Gly Ser 
    1865                 1870                 1875             


Asp Pro  Ile Leu Ser Gly Gly  Ser Lys Arg Thr Ala  Asp Gly Ser 
    1880                 1885                 1890             


Glu Phe  Glu Pro Lys Lys Lys  Arg Lys Val 
    1895                 1900             


<210>  105
<211>  2056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1(D469N)RT

<400>  105

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Ser Gly Gly Ser Ser  Gly Gly Ser Ser Gly  Ser Glu Thr 
    1385                 1390                 1395             


Pro Gly  Thr Ser Glu Ser Ala  Thr Pro Glu Ser Ser  Gly Gly Ser 
    1400                 1405                 1410             


Ser Gly  Gly Ser Ser Ala Ala  Asp Met Ser Glu Lys  Val Lys Asn 
    1415                 1420                 1425             


Gly Ile  Ile Pro Asn Phe Val  Thr Ile Pro Ser Thr  Ser Asn Asn 
    1430                 1435                 1440             


Pro Thr  Ser Ala Glu Ser Thr  Thr Asp Glu Val Ser  Glu Gln Gly 
    1445                 1450                 1455             


Glu Gln  Pro Gly Glu Val Ile  Glu Gln Gly Glu Gln  Leu Asp Glu 
    1460                 1465                 1470             


Gly Val  Glu Glu Val Glu His  Pro Thr Gln Gly Glu  Glu Gln His 
    1475                 1480                 1485             


Gln Pro  Leu Arg Arg Ser Glu  Arg Pro Arg Val Glu  Ser Arg Arg 
    1490                 1495                 1500             


Tyr Pro  Ser Thr Glu Tyr Val  Leu Ile Ser Asp Asp  Arg Glu Pro 
    1505                 1510                 1515             


Glu Ser  Leu Lys Glu Val Leu  Ser His Pro Glu Lys  Asn Gln Leu 
    1520                 1525                 1530             


Met Lys  Ala Met Gln Glu Glu  Met Glu Ser Leu Gln  Lys Asn Gly 
    1535                 1540                 1545             


Thr Tyr  Lys Leu Val Glu Leu  Pro Lys Gly Lys Arg  Pro Leu Lys 
    1550                 1555                 1560             


Cys Lys  Trp Val Phe Lys Leu  Lys Lys Asp Gly Asp  Cys Lys Leu 
    1565                 1570                 1575             


Val Arg  Tyr Lys Ala Arg Leu  Val Val Lys Gly Phe  Glu Gln Lys 
    1580                 1585                 1590             


Lys Gly  Ile Asp Phe Asp Glu  Ile Phe Ser Pro Val  Val Lys Met 
    1595                 1600                 1605             


Thr Ser  Ile Arg Thr Ile Leu  Ser Leu Ala Ala Ser  Leu Asp Leu 
    1610                 1615                 1620             


Glu Val  Glu Gln Leu Asp Val  Lys Thr Ala Phe Leu  His Gly Asp 
    1625                 1630                 1635             


Leu Glu  Glu Glu Ile Tyr Met  Glu Gln Pro Glu Gly  Phe Glu Val 
    1640                 1645                 1650             


Ala Gly  Lys Lys His Met Val  Cys Lys Leu Asn Lys  Ser Leu Tyr 
    1655                 1660                 1665             


Gly Leu  Lys Gln Ala Pro Arg  Gln Trp Tyr Met Lys  Phe Asp Ser 
    1670                 1675                 1680             


Phe Met  Lys Ser Gln Thr Tyr  Leu Lys Thr Tyr Ser  Asp Pro Cys 
    1685                 1690                 1695             


Val Tyr  Phe Lys Arg Phe Ser  Glu Asn Asn Phe Ile  Ile Leu Leu 
    1700                 1705                 1710             


Leu Tyr  Val Asp Asp Met Leu  Ile Val Gly Lys Asp  Lys Gly Leu 
    1715                 1720                 1725             


Ile Ala  Lys Leu Lys Gly Asp  Leu Ser Lys Ser Phe  Asp Met Lys 
    1730                 1735                 1740             


Asp Leu  Gly Pro Ala Gln Gln  Ile Leu Gly Met Lys  Ile Val Arg 
    1745                 1750                 1755             


Glu Arg  Thr Ser Arg Lys Leu  Trp Leu Ser Gln Glu  Lys Tyr Ile 
    1760                 1765                 1770             


Glu Arg  Val Leu Glu Arg Phe  Asn Met Lys Asn Ala  Lys Pro Val 
    1775                 1780                 1785             


Ser Thr  Pro Leu Ala Gly His  Leu Lys Leu Ser Lys  Lys Met Cys 
    1790                 1795                 1800             


Pro Thr  Thr Val Glu Glu Lys  Gly Asn Met Ala Lys  Val Pro Tyr 
    1805                 1810                 1815             


Ser Ser  Ala Val Gly Ser Leu  Met Tyr Ala Met Val  Cys Thr Arg 
    1820                 1825                 1830             


Pro Asp  Ile Ala His Ala Val  Gly Val Val Ser Arg  Phe Leu Glu 
    1835                 1840                 1845             


Asn Pro  Gly Lys Glu His Trp  Glu Ala Val Lys Trp  Ile Leu Arg 
    1850                 1855                 1860             


Tyr Leu  Arg Gly Thr Thr Gly  Asp Cys Leu Cys Phe  Gly Gly Ser 
    1865                 1870                 1875             


Asp Pro  Ile Leu Lys Gly Tyr  Thr Asn Ala Asp Met  Ala Gly Asp 
    1880                 1885                 1890             


Ile Asp  Asn Arg Lys Ser Ser  Thr Gly Tyr Leu Phe  Thr Phe Ser 
    1895                 1900                 1905             


Gly Gly  Ala Ile Ser Trp Gln  Ser Lys Leu Gln Lys  Cys Val Ala 
    1910                 1915                 1920             


Leu Ser  Thr Thr Glu Ala Glu  Tyr Ile Ala Ala Thr  Glu Thr Gly 
    1925                 1930                 1935             


Lys Glu  Met Ile Trp Leu Lys  Arg Phe Leu Gln Glu  Leu Gly Leu 
    1940                 1945                 1950             


His Gln  Lys Glu Tyr Val Val  Tyr Cys Asp Ser Gln  Ser Ala Ile 
    1955                 1960                 1965             


Asp Leu  Ser Lys Asn Ser Met  Tyr His Ala Arg Thr  Lys His Ile 
    1970                 1975                 1980             


Asp Val  Arg Tyr His Trp Ile  Arg Glu Met Val Asp  Asp Glu Ser 
    1985                 1990                 1995             


Leu Lys  Val Leu Lys Ile Ser  Thr Asn Glu Asn Pro  Ala Asp Met 
    2000                 2005                 2010             


Leu Thr  Lys Val Val Pro Arg  Asn Lys Phe Glu Leu  Cys Lys Glu 
    2015                 2020                 2025             


Leu Val  Gly Met His Ser Asn  Ser Gly Gly Ser Lys  Arg Thr Ala 
    2030                 2035                 2040             


Asp Gly  Ser Glu Phe Glu Pro  Lys Lys Lys Arg Lys  Val 
    2045                 2050                 2055     


<210>  106
<211>  617
<212>  PRT
<213>  Nicotiana tabacum

<400>  106

Ala Ala Asp Met Ser Glu Lys Val Lys Asn Gly Ile Ile Pro Asn Phe 
1               5                   10                  15      


Val Thr Ile Pro Ser Thr Ser Asn Asn Pro Thr Ser Ala Glu Ser Thr 
            20                  25                  30          


Thr Asp Glu Val Ser Glu Gln Gly Glu Gln Pro Gly Glu Val Ile Glu 
        35                  40                  45              


Gln Gly Glu Gln Leu Asp Glu Gly Val Glu Glu Val Glu His Pro Thr 
    50                  55                  60                  


Gln Gly Glu Glu Gln His Gln Pro Leu Arg Arg Ser Glu Arg Pro Arg 
65                  70                  75                  80  


Val Glu Ser Arg Arg Tyr Pro Ser Thr Glu Tyr Val Leu Ile Ser Asp 
                85                  90                  95      


Asp Arg Glu Pro Glu Ser Leu Lys Glu Val Leu Ser His Pro Glu Lys 
            100                 105                 110         


Asn Gln Leu Met Lys Ala Met Gln Glu Glu Met Glu Ser Leu Gln Lys 
        115                 120                 125             


Asn Gly Thr Tyr Lys Leu Val Glu Leu Pro Lys Gly Lys Arg Pro Leu 
    130                 135                 140                 


Lys Cys Lys Trp Val Phe Lys Leu Lys Lys Asp Gly Asp Cys Lys Leu 
145                 150                 155                 160 


Val Arg Tyr Lys Ala Arg Leu Val Val Lys Gly Phe Glu Gln Lys Lys 
                165                 170                 175     


Gly Ile Asp Phe Asp Glu Ile Phe Ser Pro Val Val Lys Met Thr Ser 
            180                 185                 190         


Ile Arg Thr Ile Leu Ser Leu Ala Ala Ser Leu Asp Leu Glu Val Glu 
        195                 200                 205             


Gln Leu Asp Val Lys Thr Ala Phe Leu His Gly Asp Leu Glu Glu Glu 
    210                 215                 220                 


Ile Tyr Met Glu Gln Pro Glu Gly Phe Glu Val Ala Gly Lys Lys His 
225                 230                 235                 240 


Met Val Cys Lys Leu Asn Lys Ser Leu Tyr Gly Leu Lys Gln Ala Pro 
                245                 250                 255     


Arg Gln Trp Tyr Met Lys Phe Asp Ser Phe Met Lys Ser Gln Thr Tyr 
            260                 265                 270         


Leu Lys Thr Tyr Ser Asp Pro Cys Val Tyr Phe Lys Arg Phe Ser Glu 
        275                 280                 285             


Asn Asn Phe Ile Ile Leu Leu Leu Tyr Val Asp Asp Met Leu Ile Val 
    290                 295                 300                 


Gly Lys Asp Lys Gly Leu Ile Ala Lys Leu Lys Gly Asp Leu Ser Lys 
305                 310                 315                 320 


Ser Phe Asp Met Lys Asp Leu Gly Pro Ala Gln Gln Ile Leu Gly Met 
                325                 330                 335     


Lys Ile Val Arg Glu Arg Thr Ser Arg Lys Leu Trp Leu Ser Gln Glu 
            340                 345                 350         


Lys Tyr Ile Glu Arg Val Leu Glu Arg Phe Asn Met Lys Asn Ala Lys 
        355                 360                 365             


Pro Val Ser Thr Pro Leu Ala Gly His Leu Lys Leu Ser Lys Lys Met 
    370                 375                 380                 


Cys Pro Thr Thr Val Glu Glu Lys Gly Asn Met Ala Lys Val Pro Tyr 
385                 390                 395                 400 


Ser Ser Ala Val Gly Ser Leu Met Tyr Ala Met Val Cys Thr Arg Pro 
                405                 410                 415     


Asp Ile Ala His Ala Val Gly Val Val Ser Arg Phe Leu Glu Asn Pro 
            420                 425                 430         


Gly Lys Glu His Trp Glu Ala Val Lys Trp Ile Leu Arg Tyr Leu Arg 
        435                 440                 445             


Gly Thr Thr Gly Asp Cys Leu Cys Phe Gly Gly Ser Asp Pro Ile Leu 
    450                 455                 460                 


Lys Gly Tyr Thr Gly Ala Asp Met Ala Gly Asp Ile Asp Asn Arg Lys 
465                 470                 475                 480 


Ser Ser Thr Gly Tyr Leu Phe Thr Phe Ser Gly Gly Ala Ile Ser Trp 
                485                 490                 495     


Gln Ser Lys Leu Gln Lys Cys Val Ala Leu Ser Thr Thr Glu Ala Gln 
            500                 505                 510         


Tyr Ile Ala Ala Thr Glu Thr Gly Lys Glu Met Ile Trp Leu Lys Arg 
        515                 520                 525             


Phe Leu Gln Glu Leu Gly Leu His Gln Lys Glu Tyr Val Val Tyr Cys 
    530                 535                 540                 


Asn Ser Gln Ser Ala Ile Asp Leu Ser Lys Asn Ser Met Tyr His Ala 
545                 550                 555                 560 


Arg Thr Lys His Ile Asp Val Arg Tyr His Trp Ile Arg Glu Met Val 
                565                 570                 575     


Asp Asp Glu Ser Leu Lys Val Leu Lys Ile Ser Thr Asn Glu Asn Pro 
            580                 585                 590         


Ala Asp Met Leu Thr Lys Val Val Pro Arg Asn Lys Phe Glu Leu Cys 
        595                 600                 605             


Lys Glu Leu Val Gly Met His Ser Asn 
    610                 615         


<210>  107
<211>  2056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1(D469G,E512Q,D545N)RT

<400>  107

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Ser Gly Gly Ser Ser  Gly Gly Ser Ser Gly  Ser Glu Thr 
    1385                 1390                 1395             


Pro Gly  Thr Ser Glu Ser Ala  Thr Pro Glu Ser Ser  Gly Gly Ser 
    1400                 1405                 1410             


Ser Gly  Gly Ser Ser Ala Ala  Asp Met Ser Glu Lys  Val Lys Asn 
    1415                 1420                 1425             


Gly Ile  Ile Pro Asn Phe Val  Thr Ile Pro Ser Thr  Ser Asn Asn 
    1430                 1435                 1440             


Pro Thr  Ser Ala Glu Ser Thr  Thr Asp Glu Val Ser  Glu Gln Gly 
    1445                 1450                 1455             


Glu Gln  Pro Gly Glu Val Ile  Glu Gln Gly Glu Gln  Leu Asp Glu 
    1460                 1465                 1470             


Gly Val  Glu Glu Val Glu His  Pro Thr Gln Gly Glu  Glu Gln His 
    1475                 1480                 1485             


Gln Pro  Leu Arg Arg Ser Glu  Arg Pro Arg Val Glu  Ser Arg Arg 
    1490                 1495                 1500             


Tyr Pro  Ser Thr Glu Tyr Val  Leu Ile Ser Asp Asp  Arg Glu Pro 
    1505                 1510                 1515             


Glu Ser  Leu Lys Glu Val Leu  Ser His Pro Glu Lys  Asn Gln Leu 
    1520                 1525                 1530             


Met Lys  Ala Met Gln Glu Glu  Met Glu Ser Leu Gln  Lys Asn Gly 
    1535                 1540                 1545             


Thr Tyr  Lys Leu Val Glu Leu  Pro Lys Gly Lys Arg  Pro Leu Lys 
    1550                 1555                 1560             


Cys Lys  Trp Val Phe Lys Leu  Lys Lys Asp Gly Asp  Cys Lys Leu 
    1565                 1570                 1575             


Val Arg  Tyr Lys Ala Arg Leu  Val Val Lys Gly Phe  Glu Gln Lys 
    1580                 1585                 1590             


Lys Gly  Ile Asp Phe Asp Glu  Ile Phe Ser Pro Val  Val Lys Met 
    1595                 1600                 1605             


Thr Ser  Ile Arg Thr Ile Leu  Ser Leu Ala Ala Ser  Leu Asp Leu 
    1610                 1615                 1620             


Glu Val  Glu Gln Leu Asp Val  Lys Thr Ala Phe Leu  His Gly Asp 
    1625                 1630                 1635             


Leu Glu  Glu Glu Ile Tyr Met  Glu Gln Pro Glu Gly  Phe Glu Val 
    1640                 1645                 1650             


Ala Gly  Lys Lys His Met Val  Cys Lys Leu Asn Lys  Ser Leu Tyr 
    1655                 1660                 1665             


Gly Leu  Lys Gln Ala Pro Arg  Gln Trp Tyr Met Lys  Phe Asp Ser 
    1670                 1675                 1680             


Phe Met  Lys Ser Gln Thr Tyr  Leu Lys Thr Tyr Ser  Asp Pro Cys 
    1685                 1690                 1695             


Val Tyr  Phe Lys Arg Phe Ser  Glu Asn Asn Phe Ile  Ile Leu Leu 
    1700                 1705                 1710             


Leu Tyr  Val Asp Asp Met Leu  Ile Val Gly Lys Asp  Lys Gly Leu 
    1715                 1720                 1725             


Ile Ala  Lys Leu Lys Gly Asp  Leu Ser Lys Ser Phe  Asp Met Lys 
    1730                 1735                 1740             


Asp Leu  Gly Pro Ala Gln Gln  Ile Leu Gly Met Lys  Ile Val Arg 
    1745                 1750                 1755             


Glu Arg  Thr Ser Arg Lys Leu  Trp Leu Ser Gln Glu  Lys Tyr Ile 
    1760                 1765                 1770             


Glu Arg  Val Leu Glu Arg Phe  Asn Met Lys Asn Ala  Lys Pro Val 
    1775                 1780                 1785             


Ser Thr  Pro Leu Ala Gly His  Leu Lys Leu Ser Lys  Lys Met Cys 
    1790                 1795                 1800             


Pro Thr  Thr Val Glu Glu Lys  Gly Asn Met Ala Lys  Val Pro Tyr 
    1805                 1810                 1815             


Ser Ser  Ala Val Gly Ser Leu  Met Tyr Ala Met Val  Cys Thr Arg 
    1820                 1825                 1830             


Pro Asp  Ile Ala His Ala Val  Gly Val Val Ser Arg  Phe Leu Glu 
    1835                 1840                 1845             


Asn Pro  Gly Lys Glu His Trp  Glu Ala Val Lys Trp  Ile Leu Arg 
    1850                 1855                 1860             


Tyr Leu  Arg Gly Thr Thr Gly  Asp Cys Leu Cys Phe  Gly Gly Ser 
    1865                 1870                 1875             


Asp Pro  Ile Leu Lys Gly Tyr  Thr Gly Ala Asp Met  Ala Gly Asp 
    1880                 1885                 1890             


Ile Asp  Asn Arg Lys Ser Ser  Thr Gly Tyr Leu Phe  Thr Phe Ser 
    1895                 1900                 1905             


Gly Gly  Ala Ile Ser Trp Gln  Ser Lys Leu Gln Lys  Cys Val Ala 
    1910                 1915                 1920             


Leu Ser  Thr Thr Glu Ala Gln  Tyr Ile Ala Ala Thr  Glu Thr Gly 
    1925                 1930                 1935             


Lys Glu  Met Ile Trp Leu Lys  Arg Phe Leu Gln Glu  Leu Gly Leu 
    1940                 1945                 1950             


His Gln  Lys Glu Tyr Val Val  Tyr Cys Asn Ser Gln  Ser Ala Ile 
    1955                 1960                 1965             


Asp Leu  Ser Lys Asn Ser Met  Tyr His Ala Arg Thr  Lys His Ile 
    1970                 1975                 1980             


Asp Val  Arg Tyr His Trp Ile  Arg Glu Met Val Asp  Asp Glu Ser 
    1985                 1990                 1995             


Leu Lys  Val Leu Lys Ile Ser  Thr Asn Glu Asn Pro  Ala Asp Met 
    2000                 2005                 2010             


Leu Thr  Lys Val Val Pro Arg  Asn Lys Phe Glu Leu  Cys Lys Glu 
    2015                 2020                 2025             


Leu Val  Gly Met His Ser Asn  Ser Gly Gly Ser Lys  Arg Thr Ala 
    2030                 2035                 2040             


Asp Gly  Ser Glu Phe Glu Pro  Lys Lys Lys Arg Lys  Val 
    2045                 2050                 2055     


<210>  108
<211>  6171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-TnT1(D469G,E512Q,D545N)RT

<400>  108
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggactccgg cggctccagc ggcggctcca gcggcagcga gacgccgggc     4200

acgtccgaga gcgccacccc cgagagcagc ggcggctcca gcggcggctc cagcgccgcg     4260

gacatgtccg agaaggtgaa gaacggcatc atcccgaact tcgtcaccat cccctccacg     4320

agcaacaacc ccacctcggc cgagtcgacg accgacgagg tgagcgagca gggcgagcag     4380

ccaggcgagg tcatcgagca gggcgagcag ctggacgagg gcgtggagga ggtggagcac     4440

ccgacccagg gcgaggagca gcaccagcca ctgaggaggt cggagcgccc gagggtggag     4500

tccaggaggt accccagcac cgagtatgtg ctcatctcgg acgacaggga gccggagtcc     4560

ctgaaggagg tgctcagcca cccggagaag aaccagctca tgaaggcgat gcaggaggag     4620

atggagtccc tgcagaagaa cggcacctac aagctggtcg agctcccgaa gggcaagagg     4680

cccctcaagt gcaagtgggt gttcaagctg aagaaggacg gcgactgcaa gctcgtccgc     4740

tacaaggcca ggctggtggt caagggcttc gagcagaaga agggcatcga cttcgacgag     4800

atcttctccc ccgtggtcaa gatgaccagc atcaggacca tcctgtcgct ggcggccagc     4860

ctggacctcg aggtggagca gctggacgtc aagaccgcct tcctgcacgg cgacctcgag     4920

gaggagatct acatggagca gcccgagggc ttcgaggtgg ccggcaagaa gcacatggtc     4980

tgcaagctga acaagtcgct ctacggcctc aagcaggccc cgaggcagtg gtacatgaag     5040

ttcgactcct tcatgaagtc ccagacctac ctcaagacgt actccgaccc gtgcgtgtac     5100

ttcaagaggt tcagcgagaa caacttcatc atcctcctgc tctacgtgga cgacatgctc     5160

atcgtcggca aggacaaggg cctgatcgcg aagctcaagg gcgacctgtc caagagcttc     5220

gacatgaagg acctcggccc ggctcagcag atcctgggca tgaagatcgt gcgcgagagg     5280

acctccagga agctgtggct cagccaggag aagtacatcg agcgcgtgct cgagaggttc     5340

aacatgaaga acgccaagcc ggtgtccacg cccctggctg gccacctgaa gctcagcaag     5400

aagatgtgcc ccaccacggt cgaggagaag ggcaacatgg ctaaggtgcc ctactcctcg     5460

gccgtgggct ccctgatgta cgctatggtg tgcacccgcc ccgacatcgc tcacgccgtg     5520

ggcgtggtca gcaggttcct cgagaacccg ggcaaggagc actgggaggc ggtgaagtgg     5580

atcctcaggt acctccgggg caccaccggc gactgcctct gcttcggtgg ctccgacccg     5640

atcctgaagg gctacaccgg cgcggacatg gccggcgaca tcgacaaccg caagtccagc     5700

accggctacc tgttcacgtt ctccggcggc gccatctcct ggcagagcaa gctccagaag     5760

tgcgtggctc tgagcacgac cgaggcccag tacatcgcgg ccacggagac gggcaaggag     5820

atgatctggc tcaagcgctt cctgcaggag ctgggcctcc accagaagga gtatgtggtc     5880

tactgcaact cccagagcgc gatcgacctg tccaagaaca gcatgtacca cgccaggacc     5940

aagcacatcg acgtgcgcta ccactggatc agggagatgg tggacgacga gtccctgaag     6000

gtcctcaaga tcagcaccaa cgagaacccc gccgacatgc tcacgaaggt ggtcccgagg     6060

aacaagttcg agctctgcaa ggagctggtc ggcatgcaca gcaactccgg cggcagcaag     6120

cgcacggcgg acggctcgga gttcgagccg aagaagaagc gcaaggtgtg a              6171


<210>  109
<211>  2125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nCas9-SH3-Tnt1-RT

<400>  109

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Gly Gly Gly Pro Arg  Ala Asp Pro Lys Lys  Lys Arg Lys 
    1385                 1390                 1395             


Val Gly  Gly Gly Pro Gly Ala  Glu Tyr Val Arg Ala  Leu Phe Asp 
    1400                 1405                 1410             


Phe Asn  Gly Asn Asp Glu Glu  Asp Leu Pro Phe Lys  Lys Gly Asp 
    1415                 1420                 1425             


Ile Leu  Arg Ile Arg Asp Lys  Pro Glu Glu Gln Trp  Trp Asn Ala 
    1430                 1435                 1440             


Glu Asp  Ser Glu Gly Lys Arg  Gly Met Ile Leu Val  Pro Tyr Val 
    1445                 1450                 1455             


Glu Lys  Tyr Ser Gly Asp Tyr  Lys Asp His Asp Gly  Asp Tyr Lys 
    1460                 1465                 1470             


Asp His  Asp Ile Asp Tyr Lys  Asp Asp Asp Asp Lys  Ser Gly Ala 
    1475                 1480                 1485             


Ala Asp  Met Ser Glu Lys Val  Lys Asn Gly Ile Ile  Pro Asn Phe 
    1490                 1495                 1500             


Val Thr  Ile Pro Ser Thr Ser  Asn Asn Pro Thr Ser  Ala Glu Ser 
    1505                 1510                 1515             


Thr Thr  Asp Glu Val Ser Glu  Gln Gly Glu Gln Pro  Gly Glu Val 
    1520                 1525                 1530             


Ile Glu  Gln Gly Glu Gln Leu  Asp Glu Gly Val Glu  Glu Val Glu 
    1535                 1540                 1545             


His Pro  Thr Gln Gly Glu Glu  Gln His Gln Pro Leu  Arg Arg Ser 
    1550                 1555                 1560             


Glu Arg  Pro Arg Val Glu Ser  Arg Arg Tyr Pro Ser  Thr Glu Tyr 
    1565                 1570                 1575             


Val Leu  Ile Ser Asp Asp Arg  Glu Pro Glu Ser Leu  Lys Glu Val 
    1580                 1585                 1590             


Leu Ser  His Pro Glu Lys Asn  Gln Leu Met Lys Ala  Met Gln Glu 
    1595                 1600                 1605             


Glu Met  Glu Ser Leu Gln Lys  Asn Gly Thr Tyr Lys  Leu Val Glu 
    1610                 1615                 1620             


Leu Pro  Lys Gly Lys Arg Pro  Leu Lys Cys Lys Trp  Val Phe Lys 
    1625                 1630                 1635             


Leu Lys  Lys Asp Gly Asp Cys  Lys Leu Val Arg Tyr  Lys Ala Arg 
    1640                 1645                 1650             


Leu Val  Val Lys Gly Phe Glu  Gln Lys Lys Gly Ile  Asp Phe Asp 
    1655                 1660                 1665             


Glu Ile  Phe Ser Pro Val Val  Lys Met Thr Ser Ile  Arg Thr Ile 
    1670                 1675                 1680             


Leu Ser  Leu Ala Ala Ser Leu  Asp Leu Glu Val Glu  Gln Leu Asp 
    1685                 1690                 1695             


Val Lys  Thr Ala Phe Leu His  Gly Asp Leu Glu Glu  Glu Ile Tyr 
    1700                 1705                 1710             


Met Glu  Gln Pro Glu Gly Phe  Glu Val Ala Gly Lys  Lys His Met 
    1715                 1720                 1725             


Val Cys  Lys Leu Asn Lys Ser  Leu Tyr Gly Leu Lys  Gln Ala Pro 
    1730                 1735                 1740             


Arg Gln  Trp Tyr Met Lys Phe  Asp Ser Phe Met Lys  Ser Gln Thr 
    1745                 1750                 1755             


Tyr Leu  Lys Thr Tyr Ser Asp  Pro Cys Val Tyr Phe  Lys Arg Phe 
    1760                 1765                 1770             


Ser Glu  Asn Asn Phe Ile Ile  Leu Leu Leu Tyr Val  Asp Asp Met 
    1775                 1780                 1785             


Leu Ile  Val Gly Lys Asp Lys  Gly Leu Ile Ala Lys  Leu Lys Gly 
    1790                 1795                 1800             


Asp Leu  Ser Lys Ser Phe Asp  Met Lys Asp Leu Gly  Pro Ala Gln 
    1805                 1810                 1815             


Gln Ile  Leu Gly Met Lys Ile  Val Arg Glu Arg Thr  Ser Arg Lys 
    1820                 1825                 1830             


Leu Trp  Leu Ser Gln Glu Lys  Tyr Ile Glu Arg Val  Leu Glu Arg 
    1835                 1840                 1845             


Phe Asn  Met Lys Asn Ala Lys  Pro Val Ser Thr Pro  Leu Ala Gly 
    1850                 1855                 1860             


His Leu  Lys Leu Ser Lys Lys  Met Cys Pro Thr Thr  Val Glu Glu 
    1865                 1870                 1875             


Lys Gly  Asn Met Ala Lys Val  Pro Tyr Ser Ser Ala  Val Gly Ser 
    1880                 1885                 1890             


Leu Met  Tyr Ala Met Val Cys  Thr Arg Pro Asp Ile  Ala His Ala 
    1895                 1900                 1905             


Val Gly  Val Val Ser Arg Phe  Leu Glu Asn Pro Gly  Lys Glu His 
    1910                 1915                 1920             


Trp Glu  Ala Val Lys Trp Ile  Leu Arg Tyr Leu Arg  Gly Thr Thr 
    1925                 1930                 1935             


Gly Asp  Cys Leu Cys Phe Gly  Gly Ser Asp Pro Ile  Leu Lys Gly 
    1940                 1945                 1950             


Tyr Thr  Asp Ala Asp Met Ala  Gly Asp Ile Asp Asn  Arg Lys Ser 
    1955                 1960                 1965             


Ser Thr  Gly Tyr Leu Phe Thr  Phe Ser Gly Gly Ala  Ile Ser Trp 
    1970                 1975                 1980             


Gln Ser  Lys Leu Gln Lys Cys  Val Ala Leu Ser Thr  Thr Glu Ala 
    1985                 1990                 1995             


Glu Tyr  Ile Ala Ala Thr Glu  Thr Gly Lys Glu Met  Ile Trp Leu 
    2000                 2005                 2010             


Lys Arg  Phe Leu Gln Glu Leu  Gly Leu His Gln Lys  Glu Tyr Val 
    2015                 2020                 2025             


Val Tyr  Cys Asp Ser Gln Ser  Ala Ile Asp Leu Ser  Lys Asn Ser 
    2030                 2035                 2040             


Met Tyr  His Ala Arg Thr Lys  His Ile Asp Val Arg  Tyr His Trp 
    2045                 2050                 2055             


Ile Arg  Glu Met Val Asp Asp  Glu Ser Leu Lys Val  Leu Lys Ile 
    2060                 2065                 2070             


Ser Thr  Asn Glu Asn Pro Ala  Asp Met Leu Thr Lys  Val Val Pro 
    2075                 2080                 2085             


Arg Asn  Lys Phe Glu Leu Cys  Lys Glu Leu Val Gly  Met His Ser 
    2090                 2095                 2100             


Asn Ser  Gly Gly Ser Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu 
    2105                 2110                 2115             


Pro Lys  Lys Lys Arg Lys Val  
    2120                 2125 


<210>  110
<211>  6378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCas9-SH3-Tnt1-RT

<400>  110
atggctccta agaagaagcg caaggttggg atccacgggg ttccggcggc tatggacaag       60

aagtacagca tcgggctcga catcgggacg aacagcgttg ggtgggcggt catcacggac      120

gagtacaagg ttccctccaa gaagttcaag gtgctgggga acaccgaccg ccactccatc      180

aagaagaacc tcatcggggc cctcctgttc gacagcgggg agaccgctga ggctacgagg      240

ctcaagagaa ccgcgaggcg cagatacacg agaaggaaga acaggatctg ctacctccaa      300

gagatcttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag      360

tctttcctcg tggaggagga caagaagcac gagcggcatc ccatcttcgg gaacatcgtc      420

gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg      480

gacagcaccg ataaggcgga cctcagactg atctacctcg ccctggcgca catgatcaag      540

ttccgcgggc atttcctgat cgagggggat ctcaacccag acaactccga tgttgacaag      600

ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgct      660

tcgggggtcg acgctaaggc catcctgtca gcccgcctct cgaagtcacg caggctggag      720

aacctgatcg cgcagctccc aggggagaag aagaacgggc tgttcgggaa cctcatcgct      780

ctctctctgg ggctcacgcc gaacttcaag tccaacttcg atctcgccga ggacgcgaag      840

ctgcaactca gcaaggacac ctacgacgat gacctcgata acctcctggc gcagatcggg      900

gatcaatacg ctgacctgtt cctcgccgcg aagaacctgt cggacgccat cctcctgtca      960

gatatcctcc gcgtgaacac cgagatcacg aaggccccac tctctgcgtc catgatcaag     1020

cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc     1080

ccggagaagt acaaggagat cttcttcgat cagtcgaaga acgggtacgc tgggtacatc     1140

gacggggggg cgtcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg     1200

gacgggacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga     1260

accttcgata acgggtccat cccccaccaa atccatctcg gggagctgca cgccatcctg     1320

agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc     1380

ctgaccttca gaatcccata ctacgtcggg ccgctcgctc gggggaacag cagattcgcc     1440

tggatgaccc gcaagtcgga ggagaccatc acgccgtgga acttcgagga ggtggtcgac     1500

aagggggcga gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc     1560

aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag     1620

ctcacgaagg tgaagtatgt gaccgagggg atgagaaagc ctgccttcct gtccggggag     1680

cagaagaagg cgatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa     1740

ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtggagat ctcaggggtc     1800

gaggacaggt tcaacgcctc cctcgggacc taccacgatc tcctgaagat catcaaggat     1860

aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc     1920

acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc     1980

gatgacaagg tcatgaagca actcaagaga agaagataca ccgggtgggg gaggctgtcc     2040

cgcaagctca tcaacgggat ccgggacaag cagtcaggga agaccatcct cgacttcctg     2100

aagtctgatg ggttcgccaa caggaacttc atgcaactga tccacgatga ctcactcacc     2160

ttcaaggagg atatccaaaa ggctcaggtg agcgggcagg gggactcgct gcacgagcat     2220

atcgctaacc tcgctgggtc ccctgcgatc aagaagggga tcctgcagac cgtgaaggtt     2280

gtggacgagc tcgtgaaggt catggggcgg cataagcctg agaacatcgt catcgagatg     2340

gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc     2400

atcgaggagg ggatcaagga gctggggtcc caaatcctca aggagcaccc agttgagaac     2460

acccaactgc agaacgagaa gctctacctg tactacctcc agaacgggag ggatatgtat     2520

gtggaccaag agctggatat caaccgcctc agcgattacg acgttgatgc tatcgtgccc     2580

cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac     2640

cgcgggaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg     2700

aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct     2760

gagagagggg ggctctcaga gctggacaag gctgggttca tcaagcggca gctggtcgag     2820

accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac     2880

gatgagaacg acaagctgat cagggaggtg aaggtcatca ccctgaagtc taagctcgtc     2940

tccgacttcc gcaaggactt ccagttctac aaggttcgcg agatcaacaa ctaccaccat     3000

gcgcatgacg cttacctcaa cgctgtggtc gggaccgccc tgatcaagaa gtacccgaag     3060

ctggagagcg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcggagc aagagatcgg gaaggccacc gccaagtact tcttctactc aaacatcatg     3180

aacttcttta agaccgagat cacgctggcc aacggggaga tccggaagag acccctcatc     3240

gagaccaacg gggagacggg ggagatcgtg tgggacaagg ggagggattt cgcgaccgtc     3300

cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggg     3360

gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag     3420

aaggactggg atccaaagaa gtacgggggg ttcgacagcc ctaccgtcgc gtactcggtt     3480

ctggttgtgg ctaaggttga gaaggggaag tccaagaagc tcaagagcgt gaaggagctc     3540

ctggggatca ccatcatgga gcgctccagc ttcgagaaga acccaatcga cttcctggag     3600

gccaaggggt acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc     3660

ttcgagctgg agaacgggag gaagcgcatg ctggcttccg ctggggagct ccaaaagggg     3720

aacgagctcg ccctgccatc caagtatgtg aacttcctct acctggcgtc ccactacgag     3780

aagctcaagg ggagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag     3840

cattacctcg acgagatcat cgagcaaatc tccgagttct ccaaacgcgt gatcctcgcg     3900

gacgctaacc tggataaggt cctctccgct tacaacaagc accgggacaa gcccatcaga     3960

gagcaggccg agaacatcat ccatctcttc accctgacga acctcggggc ccctgctgcc     4020

ttcaagtact tcgacaccac gatcgatcgg aagagataca ccagcacgaa ggaggtcctg     4080

gacgcgaccc tcatccacca atctatcacg gggctgtacg agacgagaat cgacctgtca     4140

caactggggg gggacggtgg cggccccagg gccgacccga agaagaagcg caaagtgggc     4200

ggcggccccg gcgctgagta tgtgcgcgcg ctgttcgact tcaacggcaa cgacgaggag     4260

gacctgccgt tcaagaaggg cgacatcctc cgcatcaggg acaagcccga ggagcagtgg     4320

tggaacgccg aggacagcga gggcaagagg ggcatgatcc tcgtgcccta cgtcgagaag     4380

tactccggcg actacaagga ccacgatggc gactacaagg atcacgacat cgactacaag     4440

gacgacgacg acaagtccgg cgccgcggac atgtccgaga aggtgaagaa cggcatcatc     4500

ccgaacttcg tcaccatccc ctccacgagc aacaacccca cctcggccga gtcgacgacc     4560

gacgaggtga gcgagcaggg cgagcagcca ggcgaggtca tcgagcaggg cgagcagctg     4620

gacgagggcg tggaggaggt ggagcacccg acccagggcg aggagcagca ccagccactg     4680

aggaggtcgg agcgcccgag ggtggagtcc aggaggtacc ccagcaccga gtatgtgctc     4740

atctcggacg acagggagcc ggagtccctg aaggaggtgc tcagccaccc ggagaagaac     4800

cagctcatga aggcgatgca ggaggagatg gagtccctgc agaagaacgg cacctacaag     4860

ctggtcgagc tcccgaaggg caagaggccc ctcaagtgca agtgggtgtt caagctgaag     4920

aaggacggcg actgcaagct cgtccgctac aaggccaggc tggtggtcaa gggcttcgag     4980

cagaagaagg gcatcgactt cgacgagatc ttctcccccg tggtcaagat gaccagcatc     5040

aggaccatcc tgtcgctggc ggccagcctg gacctcgagg tggagcagct ggacgtcaag     5100

accgccttcc tgcacggcga cctcgaggag gagatctaca tggagcagcc cgagggcttc     5160

gaggtggccg gcaagaagca catggtctgc aagctgaaca agtcgctcta cggcctcaag     5220

caggccccga ggcagtggta catgaagttc gactccttca tgaagtccca gacctacctc     5280

aagacgtact ccgacccgtg cgtgtacttc aagaggttca gcgagaacaa cttcatcatc     5340

ctcctgctct acgtggacga catgctcatc gtcggcaagg acaagggcct gatcgcgaag     5400

ctcaagggcg acctgtccaa gagcttcgac atgaaggacc tcggcccggc tcagcagatc     5460

ctgggcatga agatcgtgcg cgagaggacc tccaggaagc tgtggctcag ccaggagaag     5520

tacatcgagc gcgtgctcga gaggttcaac atgaagaacg ccaagccggt gtccacgccc     5580

ctggctggcc acctgaagct cagcaagaag atgtgcccca ccacggtcga ggagaagggc     5640

aacatggcta aggtgcccta ctcctcggcc gtgggctccc tgatgtacgc tatggtgtgc     5700

acccgccccg acatcgctca cgccgtgggc gtggtcagca ggttcctcga gaacccgggc     5760

aaggagcact gggaggcggt gaagtggatc ctcaggtacc tccggggcac caccggcgac     5820

tgcctctgct tcggtggctc cgacccgatc ctgaagggct acaccgacgc ggacatggcc     5880

ggcgacatcg acaaccgcaa gtccagcacc ggctacctgt tcacgttctc cggcggcgcc     5940

atctcctggc agagcaagct ccagaagtgc gtggctctga gcacgaccga ggccgagtac     6000

atcgcggcca cggagacggg caaggagatg atctggctca agcgcttcct gcaggagctg     6060

ggcctccacc agaaggagta tgtggtctac tgcgactccc agagcgcgat cgacctgtcc     6120

aagaacagca tgtaccacgc caggaccaag cacatcgacg tgcgctacca ctggatcagg     6180

gagatggtgg acgacgagtc cctgaaggtc ctcaagatca gcaccaacga gaaccccgcc     6240

gacatgctca cgaaggtggt cccgaggaac aagttcgagc tctgcaagga gctggtcggc     6300

atgcacagca actccggcgg cagcaagcgc acggcggacg gctcggagtt cgagccgaag     6360

aagaagcgca aggtgtga                                                   6378


