               SEQUENCE LISTING

<110> Adivo GmbH

<120> Modified Fc regions

<130> PCT127675-RPpau

<140> not yet assigned
<141> herewith

<150> EP20158132.9
<151> 2020-02-19

<160> 29

<170> BiSSAP 1.3.6

<210> 1
<211> 330
<212> PRT
<213> Canis lupus


<220>
<223> HC-A WT

<400> 1
Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly Ser
1               5                   10                  15
Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr Phe
            20                  25                  30
Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser Gly
        35                  40                  45
Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu His Ser Leu
    50                  55                  60
Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr Phe
65                  70                  75                  80
Thr Cys Asn Val Val His Pro Ala Ser Asn Thr Lys Val Asp Lys Pro
                85                  90                  95
Val Phe Asn Glu Cys Arg Cys Thr Asp Thr Pro Pro Cys Pro Val Pro
            100                 105                 110
Glu Pro Leu Gly Gly Pro Ser Val Leu Ile Phe Pro Pro Lys Pro Lys
        115                 120                 125
Asp Ile Leu Arg Ile Thr Arg Thr Pro Glu Val Thr Cys Val Val Leu
    130                 135                 140
Asp Leu Gly Arg Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp
145                 150                 155                 160
Gly Lys Glu Val His Thr Ala Lys Thr Gln Ser Arg Glu Gln Gln Phe
                165                 170                 175
Asn Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Glu His Gln Asp
            180                 185                 190
Trp Leu Thr Gly Lys Glu Phe Lys Cys Arg Val Asn His Ile Asp Leu
        195                 200                 205
Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Arg Ala His
    210                 215                 220
Lys Pro Ser Val Tyr Val Leu Pro Pro Ser Pro Lys Glu Leu Ser Ser
225                 230                 235                 240
Ser Asp Thr Val Ser Ile Thr Cys Leu Ile Lys Asp Phe Tyr Pro Pro
                245                 250                 255
Asp Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Gln Glu Pro Glu Arg
            260                 265                 270
Lys His Arg Met Thr Pro Pro Gln Leu Asp Glu Asp Gly Ser Tyr Phe
        275                 280                 285
Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Gln Gly Asp
    290                 295                 300
Pro Phe Thr Cys Ala Val Met His Glu Thr Leu Gln Asn His Tyr Thr
305                 310                 315                 320
Asp Leu Ser Leu Ser His Ser Pro Gly Lys
                325                 330

<210> 2
<211> 335
<212> PRT
<213> Canis lupus


<220>
<223> HC-B WT

<400> 2
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Ser Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr
            20                  25                  30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Ala His Pro Ala Ser Lys Thr Lys Val Asp Lys
                85                  90                  95
Pro Val Pro Lys Arg Glu Asn Gly Arg Val Pro Arg Pro Pro Asp Cys
            100                 105                 110
Pro Lys Cys Pro Ala Pro Glu Met Leu Gly Gly Pro Ser Val Phe Ile
        115                 120                 125
Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala Arg Thr Pro Glu
    130                 135                 140
Val Thr Cys Val Val Val Asp Leu Asp Pro Glu Asp Pro Glu Val Gln
145                 150                 155                 160
Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr Ala Lys Thr Gln
                165                 170                 175
Pro Arg Glu Glu Gln Phe Asn Gly Thr Tyr Arg Val Val Ser Val Leu
            180                 185                 190
Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln Phe Thr Cys Lys
        195                 200                 205
Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys
    210                 215                 220
Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val Leu Pro Pro Ser
225                 230                 235                 240
Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr Cys Leu Ile Lys
                245                 250                 255
Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln Ser Asn Gly Gln
            260                 265                 270
Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro Gln Leu Asp Glu
        275                 280                 285
Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg
    290                 295                 300
Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met His Glu Ala Leu
305                 310                 315                 320
His Asn His Tyr Thr Gln Glu Ser Leu Ser His Ser Pro Gly Lys
                325                 330                 335

<210> 3
<211> 333
<212> PRT
<213> Canis lupus


<220>
<223> HC-C WT

<400> 3
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Ser Gln Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr
            20                  25                  30
Ile Pro Glu Pro Val Thr Val Ser Trp Asn Ser Val Ser Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Ala His Pro Ala Thr Asn Thr Lys Val Asp Lys
                85                  90                  95
Pro Val Ala Lys Glu Cys Glu Cys Lys Cys Asn Cys Asn Asn Cys Pro
            100                 105                 110
Cys Pro Gly Cys Gly Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro
        115                 120                 125
Pro Lys Pro Lys Asp Ile Leu Val Thr Ala Arg Thr Pro Thr Val Thr
    130                 135                 140
Cys Val Val Val Asp Leu Asp Pro Glu Asn Pro Glu Val Gln Ile Ser
145                 150                 155                 160
Trp Phe Val Asp Ser Lys Gln Val Gln Thr Ala Asn Thr Gln Pro Arg
                165                 170                 175
Glu Glu Gln Ser Asn Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile
            180                 185                 190
Gly His Gln Asp Trp Leu Ser Gly Lys Gln Phe Lys Cys Lys Val Asn
        195                 200                 205
Asn Lys Ala Leu Pro Ser Pro Ile Glu Glu Ile Ile Ser Lys Thr Pro
    210                 215                 220
Gly Gln Ala His Gln Pro Asn Val Tyr Val Leu Pro Pro Ser Arg Asp
225                 230                 235                 240
Glu Met Ser Lys Asn Thr Val Thr Leu Thr Cys Leu Val Lys Asp Phe
                245                 250                 255
Phe Pro Pro Glu Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Gln Glu
            260                 265                 270
Pro Glu Ser Lys Tyr Arg Met Thr Pro Pro Gln Leu Asp Glu Asp Gly
        275                 280                 285
Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln
    290                 295                 300
Arg Gly Asp Thr Phe Ile Cys Ala Val Met His Glu Ala Leu His Asn
305                 310                 315                 320
His Tyr Thr Gln Ile Ser Leu Ser His Ser Pro Gly Lys
                325                 330

<210> 4
<211> 331
<212> PRT
<213> Canis lupus


<220>
<223> HC-D WT

<400> 4
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Ser Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr
            20                  25                  30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Thr Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Val His Pro Ala Ser Asn Thr Lys Val Asp Lys
                85                  90                  95
Pro Val Pro Lys Glu Ser Thr Cys Lys Cys Ile Ser Pro Cys Pro Val
            100                 105                 110
Pro Glu Ser Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro
        115                 120                 125
Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro Glu Ile Thr Cys Val Val
    130                 135                 140
Leu Asp Leu Gly Arg Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val
145                 150                 155                 160
Asp Gly Lys Glu Val His Thr Ala Lys Thr Gln Pro Arg Glu Gln Gln
                165                 170                 175
Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Glu His Gln
            180                 185                 190
Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys Arg Val Asn His Ile Gly
        195                 200                 205
Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Gln Ala
    210                 215                 220
His Gln Pro Ser Val Tyr Val Leu Pro Pro Ser Pro Lys Glu Leu Ser
225                 230                 235                 240
Ser Ser Asp Thr Val Thr Leu Thr Cys Leu Ile Lys Asp Phe Tyr Pro
                245                 250                 255
Pro Glu Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Pro Glu Pro Glu
            260                 265                 270
Ser Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Glu Asp Gly Ser Tyr
        275                 280                 285
Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Gln Gly
    290                 295                 300
Asp Thr Phe Thr Cys Ala Val Met His Glu Ala Leu Gln Asn His Tyr
305                 310                 315                 320
Thr Asp Leu Ser Leu Ser His Ser Pro Gly Lys
                325                 330

<210> 5
<211> 335
<212> PRT
<213> Felis catus


<220>
<223> IgG1a

<400> 5
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Thr Thr Ser Gly Ala Thr Val Ala Leu Ala Cys Leu Val Leu Gly Tyr
            20                  25                  30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ala Val Leu Gln Ala Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Leu Ser Asp Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Ala His Pro Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95
Thr Val Arg Lys Thr Asp His Pro Pro Gly Pro Lys Pro Cys Asp Cys
            100                 105                 110
Pro Lys Cys Pro Pro Pro Glu Met Leu Gly Gly Pro Ser Ile Phe Ile
        115                 120                 125
Phe Pro Pro Lys Pro Lys Asp Thr Leu Ser Ile Ser Arg Thr Pro Glu
    130                 135                 140
Val Thr Cys Leu Val Val Asp Leu Gly Pro Asp Asp Ser Asp Val Gln
145                 150                 155                 160
Ile Thr Trp Phe Val Asp Asn Thr Gln Val Tyr Thr Ala Lys Thr Ser
                165                 170                 175
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
            180                 185                 190
Pro Ile Leu His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys
        195                 200                 205
Val Asn Ser Lys Ser Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys
    210                 215                 220
Ala Lys Gly Gln Pro His Glu Pro Gln Val Tyr Val Leu Pro Pro Ala
225                 230                 235                 240
Gln Glu Glu Leu Ser Glu Asn Lys Val Ser Val Thr Cys Leu Ile Lys
                245                 250                 255
Ser Phe His Pro Pro Asp Ile Ala Val Glu Trp Glu Ile Thr Gly Gln
            260                 265                 270
Pro Glu Pro Glu Asn Asn Tyr Arg Thr Thr Pro Pro Gln Leu Asp Ser
        275                 280                 285
Asp Gly Thr Tyr Phe Val Tyr Ser Lys Leu Ser Val Asp Arg Ser His
    290                 295                 300
Trp Gln Arg Gly Asn Thr Tyr Thr Cys Ser Val Ser His Glu Ala Leu
305                 310                 315                 320
His Ser His His Thr Gln Lys Ser Leu Thr Gln Ser Pro Gly Lys
                325                 330                 335

<210> 6
<211> 335
<212> PRT
<213> Felis catus


<220>
<223> IgG1b

<400> 6
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Thr Thr Ser Gly Ala Thr Val Ala Leu Ala Cys Leu Val Leu Gly Tyr
            20                  25                  30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ala Val Leu Gln Ala Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Leu Ser Asp Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Ala His Pro Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95
Thr Val Arg Lys Thr Asp His Pro Pro Gly Pro Lys Pro Cys Asp Cys
            100                 105                 110
Pro Lys Cys Pro Pro Pro Glu Met Leu Gly Gly Pro Ser Ile Phe Ile
        115                 120                 125
Phe Pro Pro Lys Pro Lys Asp Thr Leu Ser Ile Ser Arg Thr Pro Glu
    130                 135                 140
Val Thr Cys Leu Val Val Asp Leu Gly Pro Asp Asp Ser Asp Val Gln
145                 150                 155                 160
Ile Thr Trp Phe Val Asp Asn Thr Gln Val Tyr Thr Ala Lys Thr Ser
                165                 170                 175
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
            180                 185                 190
Pro Ile Leu His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys
        195                 200                 205
Val Asn Ser Lys Ser Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys
    210                 215                 220
Asp Lys Gly Gln Pro His Glu Pro Gln Val Tyr Val Leu Pro Pro Ala
225                 230                 235                 240
Gln Glu Glu Leu Ser Glu Asn Lys Val Ser Val Thr Cys Leu Ile Glu
                245                 250                 255
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ile Thr Gly Gln
            260                 265                 270
Pro Glu Pro Glu Asn Asn Tyr Arg Thr Thr Pro Pro Gln Leu Asp Ser
        275                 280                 285
Asp Gly Thr Tyr Phe Leu Tyr Ser Arg Leu Ser Val Asp Arg Ser Arg
    290                 295                 300
Trp Gln Arg Gly Asn Thr Tyr Thr Cys Ser Val Ser His Glu Ala Leu
305                 310                 315                 320
His Ser His His Thr Gln Lys Ser Leu Thr Gln Ser Pro Gly Lys
                325                 330                 335

<210> 7
<211> 334
<212> PRT
<213> Felis catus


<220>
<223> IgG2

<400> 7
Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly
1               5                   10                  15
Thr Thr Ser Gly Ala Thr Val Ala Leu Ala Cys Leu Val Leu Gly Tyr
            20                  25                  30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45
Gly Val His Thr Phe Pro Ala Val Leu Gln Ala Ser Gly Leu Tyr Ser
    50                  55                  60
Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Leu Ser Asp Thr
65                  70                  75                  80
Phe Thr Cys Asn Val Ala His Pro Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95
Thr Val Pro Lys Thr Ala Ser Thr Ile Glu Ser Lys Thr Gly Glu Gly
            100                 105                 110
Pro Lys Cys Pro Val Pro Glu Ile Pro Gly Ala Pro Ser Val Phe Ile
        115                 120                 125
Phe Pro Pro Lys Pro Lys Asp Thr Leu Ser Ile Ser Arg Thr Pro Glu
    130                 135                 140
Val Thr Cys Leu Val Val Asp Leu Gly Pro Asp Asp Ser Asn Val Gln
145                 150                 155                 160
Ile Thr Trp Phe Val Asp Asn Thr Glu Met His Thr Ala Lys Thr Arg
                165                 170                 175
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
            180                 185                 190
Pro Ile Leu His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys
        195                 200                 205
Val Asn Ser Lys Ser Leu Pro Ser Ala Met Glu Arg Thr Ile Ser Lys
    210                 215                 220
Ala Lys Gly Gln Pro His Glu Pro Val Tyr Val Leu Pro Pro Thr Gln
225                 230                 235                 240
Glu Glu Leu Ser Glu Asn Lys Val Ser Val Thr Cys Leu Ile Lys Gly
                245                 250                 255
Phe His Pro Pro Asp Ile Ala Val Glu Trp Glu Ile Thr Gly Gln Pro
            260                 265                 270
Glu Pro Glu Asn Asn Tyr Gln Thr Thr Pro Pro Gln Leu Asp Ser Asp
        275                 280                 285
Gly Thr Tyr Phe Leu Tyr Ser Arg Leu Ser Val Asp Arg Ser His Trp
    290                 295                 300
Gln Arg Gly Asn Thr Tyr Thr Cys Ser Val Ser His Glu Ala Leu His
305                 310                 315                 320
Ser His His Thr Gln Lys Ser Leu Thr Gln Ser Pro Gly Lys
                325                 330

<210> 8
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235(A/Q/V)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<400> 8
Met Xaa Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 9
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A

<400> 9
Met Ala Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 10
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_S239(A/V)

<220>
<221> VARIANT
<222> 6
<223> X = Ala, Val

<400> 10
Met Leu Gly Gly Pro Xaa Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 11
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_S239A

<400> 11
Met Leu Gly Gly Pro Ala Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 12
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_D270(A/N)

<220>
<221> VARIANT
<222> 37
<223> X = Ala, Asn

<400> 12
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Xaa Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 13
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_D270A

<400> 13
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Ala Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 14
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_P331(G/A/S)

<220>
<221> VARIANT
<222> 98
<223> X = Gly, Ala, Ser

<400> 14
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Xaa


<210> 15
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_P331G

<400> 15
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Gly


<210> 16
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235(A/Q/V)_S239(A/V)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 6
<223> X = Ala, Val

<400> 16
Met Xaa Gly Gly Pro Xaa Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 17
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A_S239A

<400> 17
Met Ala Gly Gly Pro Ala Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 18
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235(A/Q/V)_P331(G/A/S)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 98
<223> X = Gly, Ala, Ser

<400> 18
Met Xaa Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Xaa


<210> 19
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A_P331G

<400> 19
Met Ala Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Gly


<210> 20
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_D270(A/N)_P331(G/A/S)

<220>
<221> VARIANT
<222> 37
<223> X = Ala, Asn

<220>
<221> VARIANT
<222> 98
<223> X = Gly, Ala, Ser

<400> 20
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Xaa Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Xaa


<210> 21
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_D270A_P331G

<400> 21
Met Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Ala Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Gly


<210> 22
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B HC-B_L235(A/Q/V)_S239(A/V)_D270(A/N)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 6
<223> X = Ala, Val

<220>
<221> VARIANT
<222> 37
<223> X = Ala, Asn

<400> 22
Met Xaa Gly Gly Pro Xaa Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Xaa Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 23
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A_S239A_D270A

<400> 23
Met Ala Gly Gly Pro Ala Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Ala Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 24
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235(A/Q/V)_S239(A/V)_P331(G/A/S)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 6
<223> X = Ala, Val

<220>
<221> VARIANT
<222> 98
<223> X = Gly, Ala, Ser

<400> 24
Met Xaa Gly Gly Pro Xaa Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Xaa


<210> 25
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A_S239A_P331G

<400> 25
Met Ala Gly Gly Pro Ala Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Gly


<210> 26
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B HC-B_L235(A/Q/V)_S239(A/V)_D270(A/N)_P331(G/A/S)

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 6
<223> X = Ala, Val

<220>
<221> VARIANT
<222> 37
<223> X = Ala, Asn

<220>
<221> VARIANT
<222> 98
<223> X = Gly, Ala, Ser

<400> 26
Met Xaa Gly Gly Pro Xaa Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Xaa Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Xaa


<210> 27
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_L235A_S239A_D270A_P331G

<400> 27
Met Ala Gly Gly Pro Ala Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Ala Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Gly


<210> 28
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_M234(A/Q/V) _L235(A/Q/V)

<220>
<221> VARIANT
<222> 1
<223> X = Ala, Gln, Val

<220>
<221> VARIANT
<222> 2
<223> X = Ala, Gln, Val

<400> 28
Xaa Xaa Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


<210> 29
<211> 98
<212> PRT
<213> Artificial Sequence


<220>
<223> HC-B_M234A_L235A

<400> 29
Ala Ala Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp
1               5                   10                  15
Thr Leu Leu Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            20                  25                  30
Leu Asp Pro Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly
        35                  40                  45
Lys Gln Met Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn
    50                  55                  60
Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp
65                  70                  75                  80
Leu Lys Gly Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro
                85                  90                  95
Ser Pro


