                         SEQUENCE LISTING

<110>  European Molecular Biology Laboratory
        ,
 
<120>  Archaeal pyrrolysyl tRNA synthetases for orthogonal use

<130>  M/60097-PCT

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  68
<212>  DNA
<213>  hepatitis delta virus


<220>
<221>  misc_feature
<223>  hepatitis delta virus (HDV) ribozyme (Avis)

<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  flanking sequence

<220>
<221>  misc_feature
<222>  (7)..(68)
<223>  HDV ribozyme core sequence

<400>  1
gcuagccaug gucccagccu ccucgcuggc ggcuaguggg caacaugcuu cggcauggcg       60

aaugggac                                                                68


<210>  2
<211>  67
<212>  DNA
<213>  hepatitis delta virus


<220>
<221>  misc_feature
<223>  hepatitis delta virus (HDV) ribozyme (Schuerer)

<400>  2
gggtcggcat ggcatctcca cctcctcgcg gtccgacctg ggctacttcg gtaggctaag       60

ggagaag                                                                 67


<210>  3
<211>  56
<212>  DNA
<213>  hepatitis delta virus


<220>
<221>  misc_feature
<223>  hepatitis delta virus (HDV) ribozyme (Akoopie)

<400>  3
gagggatagt acagagcctc cccgtggctc ccttggataa ccaactgata ctgtac           56


<210>  4
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  hammerhead ribozyme


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  flanking sequence

<220>
<221>  misc_feature
<222>  (11)..(45)
<223>  ribozyme core sequence

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  flanking sequence

<400>  4
ucagguuucc cugaugaguc cgugaggacg aaacgguacc cgguaccguc                  50


<210>  5
<211>  92
<212>  DNA
<213>  artificial sequence

<220>
<223>  hairpin ribozyme

<400>  5
ggagagagaa gucaaccaga gaaacacacc aacccauugc acuccggguu ggugguauau       60

uaccugguac gggggaaacu ucgugguggc cg                                     92


<210>  6
<211>  176
<212>  DNA
<213>  Neurospora intermedia


<220>
<221>  misc_feature
<223>  Varkud satellite (VS) ribozyme

<400>  6
gggaaagcuu gcgaagggcg ucgucgcccc gagcgguagu aagcagggaa cucaccucca       60

auuucaguac ugaaauuguc guagcaguug acuacuguua ugugauuggu agaggcuaag      120

ugacgguauu ggcguaaguc aguauugcag cacagcacaa gcccgcuugc gagaau          176


<210>  7
<211>  152
<212>  DNA
<213>  Bacillus spec.


<220>
<221>  misc_feature
<223>  glucosamine-6-phosphate synthetase (glmS) riboswitch (glmS 
       ribozyme)

<400>  7
gcgccagaac tggcaccauu gcacuccggu gccaguugac gagguggggu uuaucgagau       60

uucggcggau gacucccggu uguucaucac aaccgcaagc uuuuacuuaa aucauuaagg      120

ugacuuagug gacaaaggug aaagugugau ga                                    152


<210>  8
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  tetracycline operator (tetO)

<400>  8
tccctatcag tgatagaga                                                    19


<210>  9
<211>  59
<212>  DNA
<213>  artificial sequence

<220>
<223>  minimal cytomegalovirus (CMV) promoter

<400>  9
gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatc        59


<210>  10
<211>  467
<212>  DNA
<213>  Artificial sequence

<220>
<223>  "Tet-On" promoter comprising 8 tetO sequences


<220>
<221>  misc_feature
<222>  (18)..(36)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (54)..(72)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (90)..(208)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (126)..(144)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (162)..(180)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (198)..(216)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (234)..(252)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (270)..(288)
<223>  tetO sequence

<400>  10
caattgctcg agtttactcc ctatcagtga tagagaacgt atgtcgactt tactccctat       60

cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac      120

tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga      180

acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat      240

cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatacgctt      300

taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc      360

tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg      420

aaagtccata tggctagcct gcagggatcc agatatcaag cttcacc                    467


<210>  11
<211>  425
<212>  DNA
<213>  Artificial sequence

<220>
<223>  "Tet-On" promoter comprising 7 tetO sequences


<220>
<221>  misc_feature
<222>  (12)..(30)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (48)..(66)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (84)..(102)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (120)..(138)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (156)..(174)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (192)..(210)
<223>  tetO sequences

<220>
<221>  misc_feature
<222>  (228)..(246)
<223>  tetO sequences

<400>  11
ctcgacttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga       60

tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact      120

ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat      180

ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga      240

tagagaacgt atacgcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta      300

gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg      360

taccacttcc taccctcgaa agtccatatg gctagcctgc agggatccag atatcaagct      420

tcacc                                                                  425


<210>  12
<211>  794
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  "T-REx" promoter comprising two tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(380)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (381)..(584)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (586)..(604)
<223>  tetO sequence

<220>
<221>  misc_feature
<222>  (607)..(625)
<223>  tetO sequence

<400>  12
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata      600

gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg      660

cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc      720

tccggactct agcgtttaaa catcacaagt ttgtacaaaa aagcaggcgc tagcgtttaa      780

acttaagctt cacc                                                        794


<210>  13
<211>  207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tetracycline repressor (tetR) UniProtKB/Swiss-Prot accession no.:
       P04483.1

<400>  13

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser 
        195                 200                 205         


<210>  14
<211>  1442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rtTA expression cassette


<220>
<221>  misc_feature
<222>  (1)..(588)
<223>  CMV

<220>
<221>  misc_feature
<222>  (696)..(1442)
<223>  TetOn3G

<400>  14
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta      600

gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag      660

ctggctagcg tttaaactta agcttctcga gcaccatgtc tagactggac aagagcaaag      720

tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga      780

aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc      840

gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc      900

ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg      960

ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac     1020

agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga     1080

acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac     1140

aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac     1200

ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg     1260

gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga     1320

ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg     1380

accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt     1440

aa                                                                    1442


<210>  15
<211>  18
<212>  DNA
<213>  artificial sequence derived from enterobacteria phage T7 promoter

<220>
<223>  T7 promoter class III (core)


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  G at position 18 is preferred nucleotide at the +1 position to 
       promote efficient transcription initiation

<400>  15
taatacgact cactatag                                                     18


<210>  16
<211>  19
<212>  DNA
<213>  artificial sequence derived from enterobacteria phage T7 promoter

<220>
<223>  T7 promoter class III


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  GG at positions 18 and 19 are preferred nucleotides at the +1 and
       +2 positions to promote efficient transcription initiation

<400>  16
taatacgact cactatagg                                                    19


<210>  17
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  T7 promoter class II (core)


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  A at position 18 is preferred nucleotide at the +1 position of 
       the gene to promote efficient transcription initiation

<400>  17
taatacgact cactatta                                                     18


<210>  18
<211>  883
<212>  PRT
<213>  Enterobacteria phage T7

<400>  18

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             


Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 


Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         


Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
305                 310                 315                 320 


Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
                325                 330                 335     


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
        355                 360                 365             


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 


Ala Phe Ala 
            


<210>  19
<211>  899
<212>  PRT
<213>  artificial sequence derived from Enterobacteria phage T7

<220>
<223>  NES-T7 RNA polymerase


<220>
<221>  MISC_FEATURE
<222>  (2)..(17)
<223>  NES

<400>  19

Met Cys Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu 
1               5                   10                  15      


Asp Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
            20                  25                  30          


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
        35                  40                  45              


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
    50                  55                  60                  


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
65                  70                  75                  80  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
                85                  90                  95      


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
            100                 105                 110         


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
        115                 120                 125             


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
    130                 135                 140                 


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
145                 150                 155                 160 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
                165                 170                 175     


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
            180                 185                 190         


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
        195                 200                 205             


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
    210                 215                 220                 


Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
225                 230                 235                 240 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
                245                 250                 255     


Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
            260                 265                 270         


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
        275                 280                 285             


Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
    290                 295                 300                 


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
305                 310                 315                 320 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
                325                 330                 335     


Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
            340                 345                 350         


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
        355                 360                 365             


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
    370                 375                 380                 


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
385                 390                 395                 400 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
                405                 410                 415     


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
            420                 425                 430         


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
        435                 440                 445             


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
    450                 455                 460                 


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
465                 470                 475                 480 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
                485                 490                 495     


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
            500                 505                 510         


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
        515                 520                 525             


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
    530                 535                 540                 


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
545                 550                 555                 560 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
                565                 570                 575     


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
            580                 585                 590         


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
        595                 600                 605             


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
    610                 615                 620                 


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
625                 630                 635                 640 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
                645                 650                 655     


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
            660                 665                 670         


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
        675                 680                 685             


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
    690                 695                 700                 


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
705                 710                 715                 720 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
                725                 730                 735     


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
            740                 745                 750         


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
        755                 760                 765             


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
    770                 775                 780                 


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
785                 790                 795                 800 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
                805                 810                 815     


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
            820                 825                 830         


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
        835                 840                 845             


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
    850                 855                 860                 


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
865                 870                 875                 880 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
                885                 890                 895     


Ala Phe Ala 
            


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS consensus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is selected from K and R

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any naturally occuring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is selected from K and R

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  20

Lys Xaa Xaa Xaa 
1               


<210>  21
<211>  7
<212>  PRT
<213>  simian vacuolating virus 40 (SV40)

<400>  21

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  22
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS Y306A, Y384F mutant (PylRSAF)


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  ATG coding for M may be missing

<400>  22
atggcgtgcc cggtgccgct gcagctgccg ccgctggaac gcctgaccct ggatgataaa       60

aaaccgctga ataccctgat ctctgctact ggtctgtgga tgagtcgtac cggaaccatt      120

cataaaatca aacaccacga ggttagccgt tcgaaaatct atattgagat ggcgtgtggc      180

gatcatctgg ttgtgaacaa tagccgctct tctcgtacag cacgtgcact gcgtcaccac      240

aaatatcgta aaacctgtaa acgttgccgt gtgtccgatg aggatctgaa caaattcctg      300

acaaaagcca atgaggacca aacaagcgtg aaagtgaaag tcgttagcgc tcctacccgt      360

actaaaaaag caatgccgaa atccgttgct cgtgccccta aaccactgga aaacactgaa      420

gcagcacagg cacagccgtc tggaagcaaa ttctctccgg ccattcctgt ttctacccag      480

gagtccgttt ctgttccagc aagtgtgagc accagcatta gcagtattag caccggtgcc      540

accgctagcg ccctggttaa aggcaatacc aatccgatta caagcatgtc tgccccggtt      600

caagcatcag ctccagcact gacaaaatcc caaaccgatc gtctggaggt tctgctgaat      660

ccgaaagacg aaatcagcct gaattccggc aaaccgtttc gtgaactgga gagcgaactg      720

ctgtcacgtc gtaaaaaaga cctgcaacaa atctatgccg aagaacgtga gaactatctg      780

gggaaactgg aacgtgaaat cacccgcttt ttcgtggatc gtggctttct ggagatcaaa      840

tccccgattc tgattcctct ggagtatatc gagcgtatgg gcatcgacaa tgataccgaa      900

ctgagcaaac aaattttccg tgtggataaa aacttctgtc tgcgccctat gctagcacca      960

aatctggcta actatctgcg caaactggac cgtgccctgc ctgatcctat caaaatcttc     1020

gagatcggcc cgtgttatcg taaagagtcc gacggtaaag aacatctgga ggagtttacc     1080

atgctgaact tttgccaaat gggttcaggt tgtactcgtg agaacctgga aagcatcatc     1140

accgattttc tgaaccacct gggcattgac ttcaaaattg tgggcgacag ctgtatggtg     1200

tttggcgaca ccctggatgt catgcacggc gacctggaac tgtctagtgc cgttgtgggc     1260

ccaatcccgc tggatcgtga gtggggtatc gacaaacctt ggatcggtgc gggttttggt     1320

ctggagcgtc tgctgaaagt aaaacacgac ttcaagaaca tcaaacgtgc tgcacgttcc     1380

gagtcctatt acaatggtat ttctactaac ctgtaa                               1416


<210>  23
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS N346A, C348A mutant (PylRSAA)

<400>  23
atggcgtgcc cggtgccgct gcagctgccg ccgctggaac gcctgaccct ggatgacaaa       60

aaaccgctga ataccctgat ctctgctact ggtctgtgga tgagtcgtac cggaaccatt      120

cataaaatca aacaccacga ggttagccgt tcgaaaatct atattgagat ggcgtgtggc      180

gatcatctgg ttgtgaacaa tagccgctct tctcgtacag cacgtgcact gcgtcaccac      240

aaatatcgta aaacctgtaa acgttgccgt gtgtccgatg aggatctgaa caaattcctg      300

acaaaagcca atgaggacca aacaagcgtg aaagtgaaag tcgttagcgc tcctacccgt      360

actaaaaaag caatgccgaa atccgttgct cgtgccccta aaccactgga aaacactgaa      420

gcagcacagg cacagccgtc tggaagcaaa ttctctccgg ccattcctgt ttctacccag      480

gagtccgttt ctgttccagc aagtgtgagc accagcatta gcagtattag caccggtgcc      540

accgctagcg ccctggttaa aggcaatacc aatccgatta caagcatgtc tgccccggtt      600

caagcatcag ctccagcact gacaaaatcc caaaccgatc gtctggaggt tctgctgaat      660

ccgaaagacg aaatcagcct gaattccggc aaaccgtttc gtgaactgga gagcgaactg      720

ctgtcacgtc gtaaaaaaga cctgcaacaa atctatgccg aagaacgtga gaactatctg      780

gggaaactgg aacgtgaaat cacccgcttt ttcgtggatc gtggctttct ggagatcaaa      840

tccccgattc tgattcctct ggagtatatc gagcgtatgg gcatcgacaa tgataccgaa      900

ctgagcaaac aaattttccg tgtggataaa aacttctgtc tgcgccctat gctggcacca      960

aatctgtata actatctgcg caaactggac cgtgccctgc ctgatcctat caaaatcttc     1020

gagatcggcc cgtgttatcg taaagagtcc gacggtaaag aacatctgga ggagtttacc     1080

atgctggcct ttgcccaaat gggttcaggt tgtactcgtg agaacctgga aagcatcatc     1140

accgattttc tgaaccacct gggcattgac ttcaaaattg tgggcgacag ctgtatggtg     1200

tatggcgaca ccctggatgt catgcacggc gacctggaac tgtctagtgc cgttgttgga     1260

ccaattccgc tggaccgtga gtggggtatc gacaaaccgt ggatcggagc aggattcggt     1320

ctggaacgcc tgctgaaagt gaaacacgac ttcaaaaaca tcaaacgtgc cgcccgttct     1380

gaatcgtatt ataacgggat ctctacgaac ctgtaa                               1416


<210>  24
<211>  1413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS Y306A, N346A, C348A, Y384Fmutant 
       (PylRSAAAF)

<400>  24
gcgtgcccgg tgccgctgca gctgccgccg ctggaacgcc tgaccctgga tgataaaaaa       60

ccgctgaata ccctgatctc tgctactggt ctgtggatga gtcgtaccgg aaccattcat      120

aaaatcaaac accacgaggt tagccgttcg aaaatctata ttgagatggc gtgtggcgat      180

catctggttg tgaacaatag ccgctcttct cgtacagcac gtgcactgcg tcaccacaaa      240

tatcgtaaaa cctgtaaacg ttgccgtgtg tccgatgagg atctgaacaa attcctgaca      300

aaagccaatg aggaccaaac aagcgtgaaa gtgaaagtcg ttagcgctcc tacccgtact      360

aaaaaagcaa tgccgaaatc cgttgctcgt gcccctaaac cactggaaaa cactgaagca      420

gcacaggcac agccgtctgg aagcaaattc tctccggcca ttcctgtttc tacccaggag      480

tccgtttctg ttccagcaag tgtgagcacc agcattagca gtattagcac cggtgccacc      540

gctagcgccc tggttaaagg caataccaat ccgattacaa gcatgtctgc cccggttcaa      600

gcatcagctc cagcactgac aaaatcccaa accgatcgtc tggaggttct gctgaatccg      660

aaagacgaaa tcagcctgaa ttccggcaaa ccgtttcgtg aactggagag cgaactgctg      720

tcacgtcgta aaaaagacct gcaacaaatc tatgccgaag aacgtgagaa ctatctgggg      780

aaactggaac gtgaaatcac ccgctttttc gtggatcgtg gctttctgga gatcaaatcc      840

ccgattctga ttcctctgga gtatatcgag cgtatgggca tcgacaatga taccgaactg      900

agcaaacaaa ttttccgtgt ggataaaaac ttctgtctgc gccctatgct agcaccaaat      960

ctggctaact atctgcgcaa actggaccgt gccctgcctg atcctatcaa aatcttcgag     1020

atcggcccgt gttatcgtaa agagtccgac ggtaaagaac atctggagga gtttaccatg     1080

ctggcctttg cccaaatggg ttcaggttgt actcgtgaga acctggaaag catcatcacc     1140

gattttctga accacctggg cattgacttc aaaattgtgg gcgacagctg tatggtgttt     1200

ggcgacaccc tggatgtcat gcacggcgac ctggaactgt ctagtgccgt tgtgggccca     1260

atcccgctgg atcgtgagtg gggtatcgac aaaccttgga tcggtgcggg ttttggtctg     1320

gagcgtctgc tgaaagtaaa acacgacttc aagaacatca aacgtgctgc acgttccgag     1380

tcctattaca atggtatttc tactaacctg taa                                  1413


<210>  25
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA of Methanosarcina mazei for Amber codon 
       (tRNAPyl,CUA)


<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  anticodon

<400>  25
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg       60

gggtttccg                                                               69


<210>  26
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA of Methanosarcina mazei for Opal codon 
       (tRNAPyl,UCA)


<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  anticodon

<400>  26
ggaaacctga tcatgtagat cgaatggact tcaaatccgt tcagccgggt tagattcccg       60

gggtttccg                                                               69


<210>  27
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA of Methanosarcina mazei for Ochre codon 
       (tRNAPyl,UUA)


<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  anticodon

<400>  27
ggaaacctga tcatgtagat cgaatggact ttaaatccgt tcagccgggt tagattcccg       60

gggtttccg                                                               69


<210>  28
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<400>  28

Xaa Xaa Xaa Xaa Xaa Xaa 
1               5       


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (2)..(4)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<400>  29

Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5           


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is selected from Leu and Ile

<400>  30

Leu Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa 
1               5                   


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is selected from any canonical amino acid

<400>  31

Leu Xaa Xaa Xaa Xaa Xaa Leu Xaa 
1               5               


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (5)..(7)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is selected from any canonical amino acid

<400>  32

Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 
1               5                   


<210>  33
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(4)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is selected from Leu, Ile, Val, Phe and Met

<220>
<221>  MISC_FEATURE
<222>  (6)..(8)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is selected from any canonical amino acid

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is selected from Leu and Ile

<400>  33

Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa 
1               5                   10      


<210>  34
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif

<400>  34

Leu Pro Pro Leu Glu Arg Leu Thr Leu 
1               5                   


<210>  35
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES motif

<400>  35

Ala Cys Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu 
1               5                   10                  15      


Asp 
    


<210>  36
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-PKR(2-174)


<220>
<221>  MISC_FEATURE
<222>  (2)..(10)
<223>  HA

<220>
<221>  MISC_FEATURE
<222>  (11)..(183)
<223>  human PKR(2-174)

<400>  36

Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ala Gly Asp Leu Ser Ala 
1               5                   10                  15      


Gly Phe Phe Met Glu Glu Leu Asn Thr Tyr Arg Gln Lys Gln Gly Val 
            20                  25                  30          


Val Leu Lys Tyr Gln Glu Leu Pro Asn Ser Gly Pro Pro His Asp Arg 
        35                  40                  45              


Arg Phe Thr Phe Gln Val Ile Ile Asp Gly Arg Glu Phe Pro Glu Gly 
    50                  55                  60                  


Glu Gly Arg Ser Lys Lys Glu Ala Lys Asn Ala Ala Ala Lys Leu Ala 
65                  70                  75                  80  


Val Glu Ile Leu Asn Lys Glu Lys Lys Ala Val Ser Pro Leu Leu Leu 
                85                  90                  95      


Thr Thr Thr Asn Ser Ser Glu Gly Leu Ser Met Gly Asn Tyr Ile Gly 
            100                 105                 110         


Leu Ile Asn Arg Ile Ala Gln Lys Lys Arg Leu Thr Val Asn Tyr Glu 
        115                 120                 125             


Gln Cys Ala Ser Gly Val His Gly Pro Glu Gly Phe His Tyr Lys Cys 
    130                 135                 140                 


Lys Met Gly Gln Lys Glu Tyr Ser Ile Gly Thr Gly Ser Thr Lys Gln 
145                 150                 155                 160 


Glu Ala Lys Gln Leu Ala Ala Lys Leu Ala Tyr Leu Gln Ile Leu Ser 
                165                 170                 175     


Glu Glu Thr Ser Val Lys Ser 
            180             


<210>  37
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  core of dsRNA-binding domain of PKR


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  restricted; not hydrophobic (i.e. K, R, H, D, E, S, T, N, Q, C, 
       preferably , R, S, C, Q, H, (K))

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  restricted; not hydrophobic (i.e. K, R, H, D, E, S, T, N, Q, C, 
       preferably, Q, (N))

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (48)..(48)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (55)..(55)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (59)..(59)
<223>  restricted; A59 is not D; i.e. A59 is A, G, I, L, M, V

<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  restricted; L66 is not A; i.e. L66 is L, I, V, M

<220>
<221>  MISC_FEATURE
<222>  (122)..(122)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (141)..(141)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  conserved

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  restricted; A149 is not D; i.e. A149 is A, G, I, L, M, V

<400>  37

Phe Met Glu Glu Leu Asn Thr Tyr Arg Gln Lys Gln Gly Val Val Leu 
1               5                   10                  15      


Lys Tyr Gln Glu Leu Pro Asn Ser Gly Pro Pro His Asp Arg Arg Phe 
            20                  25                  30          


Thr Phe Gln Val Ile Ile Asp Gly Arg Glu Phe Pro Glu Gly Glu Gly 
        35                  40                  45              


Arg Ser Lys Lys Glu Ala Lys Asn Ala Ala Ala Lys Leu Ala Val Glu 
    50                  55                  60                  


Ile Leu Asn Lys Glu Lys Lys Ala Val Ser Pro Leu Leu Leu Thr Thr 
65                  70                  75                  80  


Thr Asn Ser Ser Glu Gly Leu Ser Met Gly Asn Tyr Ile Gly Leu Ile 
                85                  90                  95      


Asn Arg Ile Ala Gln Lys Lys Arg Leu Thr Val Asn Tyr Glu Gln Cys 
            100                 105                 110         


Ala Ser Gly Val His Gly Pro Glu Gly Phe His Tyr Lys Cys Lys Met 
        115                 120                 125             


Gly Gln Lys Glu Tyr Ser Ile Gly Thr Gly Ser Thr Lys Gln Glu Ala 
    130                 135                 140                 


Lys Gln Leu Ala Ala Lys Leu Ala Tyr Leu Gln Ile Leu Ser Glu 
145                 150                 155                 


<210>  38
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (full) dsRNA-binding domain of PKR - M1

<400>  38

Ala Gly Asp Leu Ser Ala Gly Phe Phe Met Glu Glu Leu Asn Thr Tyr 
1               5                   10                  15      


Arg Gln Lys Gln Gly Val Val Leu Lys Tyr Gln Glu Leu Pro Asn Ser 
            20                  25                  30          


Gly Pro Pro His Asp Arg Arg Phe Thr Phe Gln Val Ile Ile Asp Gly 
        35                  40                  45              


Arg Glu Phe Pro Glu Gly Glu Gly Arg Ser Lys Lys Glu Ala Lys Asn 
    50                  55                  60                  


Ala Ala Ala Lys Leu Ala Val Glu Ile Leu Asn Lys Glu Lys Lys Ala 
65                  70                  75                  80  


Val Ser Pro Leu Leu Leu Thr Thr Thr Asn Ser Ser Glu Gly Leu Ser 
                85                  90                  95      


Met Gly Asn Tyr Ile Gly Leu Ile Asn Arg Ile Ala Gln Lys Lys Arg 
            100                 105                 110         


Leu Thr Val Asn Tyr Glu Gln Cys Ala Ser Gly Val His Gly Pro Glu 
        115                 120                 125             


Gly Phe His Tyr Lys Cys Lys Met Gly Gln Lys Glu Tyr Ser Ile Gly 
    130                 135                 140                 


Thr Gly Ser Thr Lys Gln Glu Ala Lys Gln Leu Ala Ala Lys Leu Ala 
145                 150                 155                 160 


Tyr Leu Gln Ile Leu Ser Glu Glu Thr Ser Val Lys Ser 
                165                 170             


<210>  39
<211>  2769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette for reporter gene 
       NLS-iRFP-Flag-GFPY39->TAG-6His


<220>
<221>  misc_feature
<222>  (1)..(852)
<223>  promoter

<220>
<221>  misc_feature
<222>  (949)..(984)
<223>  NLS

<220>
<221>  misc_feature
<222>  (988)..(1929)
<223>  iRFP

<220>
<221>  misc_feature
<222>  (1930)..(2004)
<223>  25 Helix

<220>
<221>  misc_feature
<222>  (2011)..(2034)
<223>  Flag

<220>
<221>  misc_feature
<222>  (2035)..(2748)
<223>  GFPY39 CDS

<220>
<221>  misc_feature
<222>  (2149)..(2151)
<223>  Y39-encoding triplet

<220>
<221>  misc_feature
<222>  (2749)..(2766)
<223>  6His

<400>  39
ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc      600

agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg      660

cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg      720

taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac      780

agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct      840

ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat      900

acgactcact ataggctagc caccatgggc cgcctggaaa gcaccccgcc gaaaaaaaaa      960

cgcaaagtgg aagatagcgc gagcgcggaa ggatccgtcg ccaggcagcc tgacctcttg     1020

acctgcgacg atgagccgat ccatatcccc ggtgccatcc aaccgcatgg actgctgctc     1080

gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca accttcccga actcaccgga     1140

ctggcgatcg gcgccctgat cggccgctct gcggccgatg tcttcgactc ggagacgcac     1200

aaccgtctga cgatcgcctt ggccgagccc ggggcggccg tcggagcacc gatcactgtc     1260

ggcttcacga tgcgaaagga cgcaggcttc atcggctcct ggcatcgcca tgatcagctc     1320

atcttcctcg agctcgagcc tccccagcgg gacgtcgccg agccgcaggc gttcttccgc     1380

cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa ccttggaaag cgcctgcgcc     1440

gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc gggtgatgat ctatcgcttc     1500

gcctccgact tcagcggcga agtgatcgca gaggatcggt gcgccgaggt cgagtcaaaa     1560

ctaggcctgc actatcctgc ctcaaccgtg ccggcgcagg cccgtcggct ctataccatc     1620

aacccggtac ggatcattcc cgatatcaat tatcggccgg tgccggtcac cccagacctc     1680

aatccggtca ccgggcggcc gattgatctt agcttcgcca tcctgcgcag cgtctcgccc     1740

gtccatctgg aattcatgcg caacataggc atgcacggca cgatgtcgat ctcgattttg     1800

cgcggcgagc gactgtgggg attgatcgtt tgccatcacc gaacgccgta ctacgtcgat     1860

ctcgatggcc gccaagcctg cgagctagtc gcccaggttc tggcctggca gatcggcgtg     1920

atggaagagc tggcggaagc ggcggccaaa gaagcggcgg ccaaagaagc ggcggccaaa     1980

gaagcggcgg ccaaagcggc ggccggtacc gattacaaag atgatgatga taaagtgagc     2040

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2100

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta gggcaagctg     2160

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2220

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2280

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2340

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     2400

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     2460

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     2520

gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     2580

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     2640

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     2700

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagca tcaccatcac     2760

catcactga                                                             2769


<210>  40
<211>  3341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase expression cassette for constitutive expression
       via CMV promoter


<220>
<221>  misc_feature
<222>  (1)..(588)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (690)..(3341)
<223>  CDS

<400>  40
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta      600

gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag      660

ctggctagcg tttaaactta agcttcacca tgaacacgat taacatcgct aagaacgact      720

tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc      780

gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct      840

tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca      900

agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg      960

aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc     1020

cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca     1080

atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct     1140

tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca     1200

acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc     1260

tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc     1320

atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc     1380

gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg     1440

ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt     1500

gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc     1560

gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg     1620

tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca     1680

acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg     1740

acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc     1800

ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc     1860

gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc     1920

ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa     1980

tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac     2040

caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg     2100

ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg     2160

cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct     2220

tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct     2280

cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc     2340

gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct     2400

acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg     2460

ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc     2520

tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta     2580

agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc     2640

tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga     2700

atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag     2760

ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca     2820

aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg     2880

atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt     2940

tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg     3000

cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc     3060

ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc     3120

acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa     3180

ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg     3240

accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga     3300

acctccgtga catcttagag tcggacttcg cgttcgcgta a                         3341


<210>  41
<211>  3494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NES-T7 RNA polymerase expression cassette containing "T-REx" 
       promoter comprising two tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(584)
<223>  promoter

<220>
<221>  misc_feature
<222>  (586)..(604)
<223>  tetO

<220>
<221>  misc_feature
<222>  (607)..(625)
<223>  tetO

<220>
<221>  misc_feature
<222>  (798)..(845)
<223>  NES

<220>
<221>  misc_feature
<222>  (846)..(3494)
<223>  CDS

<400>  41
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata      600

gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg      660

cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc      720

tccggactct agcgtttaaa catcacaagt ttgtacaaaa aagcaggcgc tagcgtttaa      780

acttaagctt caccatgtgc ccggtgccgc tgcagctgcc gccgctggaa cgcctgaccc      840

tggataacac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc      900

cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg      960

agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta     1020

aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta     1080

agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc     1140

cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta     1200

agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg     1260

caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta     1320

agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga     1380

aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg     1440

cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc     1500

tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag     1560

actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg     1620

cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg     1680

gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc     1740

acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga     1800

ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg     1860

taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac     1920

tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg     1980

ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt     2040

tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca     2100

tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga     2160

ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc     2220

tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca     2280

agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca     2340

cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg     2400

gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt     2460

gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta     2520

acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg     2580

agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg     2640

agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat     2700

ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg     2760

ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg     2820

attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc     2880

tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta     2940

agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc     3000

gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca     3060

agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta     3120

ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc     3180

ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg     3240

agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg     3300

acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg     3360

atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca     3420

aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact     3480

tcgcgttcgc gtaa                                                       3494


<210>  42
<211>  3169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NES-T7 RNA polymerase expression cassette containing "Tet-On" 
       promoter comprising 8 tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(458)
<223>  promoter

<220>
<221>  misc_feature
<222>  (18)..(36)
<223>  tetO

<220>
<221>  misc_feature
<222>  (54)..(72)
<223>  tetO

<220>
<221>  misc_feature
<222>  (90)..(108)
<223>  tetO

<220>
<221>  misc_feature
<222>  (126)..(144)
<223>  tetO

<220>
<221>  misc_feature
<222>  (162)..(180)
<223>  tetO

<220>
<221>  misc_feature
<222>  (198)..(216)
<223>  tetO

<220>
<221>  misc_feature
<222>  (234)..(252)
<223>  tetO

<220>
<221>  misc_feature
<222>  (270)..(288)
<223>  tetO

<220>
<221>  misc_feature
<222>  (473)..(520)
<223>  NES

<220>
<221>  misc_feature
<222>  (521)..(3169)
<223>  CDS

<400>  42
caattgctcg agtttactcc ctatcagtga tagagaacgt atgtcgactt tactccctat       60

cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac      120

tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga      180

acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat      240

cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatacgctt      300

taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc      360

tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg      420

aaagtccata tggctagcct gcagggatcc agatatcaag cttgccacca tgtgcccggt      480

gccgctgcag ctgccgccgc tggaacgcct gaccctggat aacacgatta acatcgctaa      540

gaacgacttc tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta      600

cggtgagcgt ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga      660

agcacgcttc cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc      720

tgccgccaag cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg      780

gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga      840

aatcaagccg gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag      900

tgctgacaat acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga      960

ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga     1020

acaactcaac aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc     1080

tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga     1140

ctctattcat gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag     1200

cttacaccgc caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc     1260

tgaatacgct gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt     1320

ccaaccttgc gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc     1380

taacggtcgt cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta     1440

cgaagacgtt tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg     1500

gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc     1560

ggtcgaggac atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga     1620

catgaatcct gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga     1680

caaggctcgc aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt     1740

tgctaaccat aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc     1800

tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa     1860

aggtaaacca atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc     1920

gggtgtcgat aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa     1980

catcatggct tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc     2040

gttctgcttc cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta     2100

taactgctcc cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc     2160

gatgctccga gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca     2220

ggacatctac gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa     2280

tgggaccgat aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa     2340

agtcaagctg ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag     2400

tgtgactaag cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca     2460

acaagtgctg gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac     2520

tcagccgaat caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac     2580

ggtggtagct gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc     2640

tgaggtcaaa gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt     2700

aactcctgat ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa     2760

cctgatgttc ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga     2820

gattgatgca cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg     2880

tagccacctt cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc     2940

actgattcac gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt     3000

gcgcgaaact atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca     3060

gttcgctgac cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg     3120

taacttgaac ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaa                 3169


<210>  43
<211>  3389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NES-T7 RNA polymerase expression cassette for constitutive 
       expression via CMV promoter


<220>
<221>  misc_feature
<222>  (1)..(588)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (693)..(740)
<223>  NES

<220>
<221>  misc_feature
<222>  (741)..(3389)
<223>  CDS

<400>  43
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta      600

gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag      660

ctggctagcg tttaaactta agcttcacca tgtgcccggt gccgctgcag ctgccgccgc      720

tggaacgcct gaccctggat aacacgatta acatcgctaa gaacgacttc tctgacatcg      780

aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg      840

aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt      900

ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca      960

ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta     1020

agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag     1080

cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc     1140

aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc     1200

gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag     1260

ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc     1320

tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac     1380

gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg     1440

gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg     1500

caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc     1560

ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg     1620

cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg     1680

aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc     1740

tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga     1800

ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca     1860

ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc     1920

gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct     1980

ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc     2040

aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg     2100

aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt     2160

tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt     2220

ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct     2280

gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg     2340

cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag     2400

gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg     2460

ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag     2520

ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg     2580

cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca     2640

tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca     2700

ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg     2760

gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag     2820

caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga     2880

ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg     2940

tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt     3000

tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg     3060

agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg     3120

tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg     3180

gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca     3240

catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg     3300

agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca     3360

tcttagagtc ggacttcgcg ttcgcgtaa                                       3389


<210>  44
<211>  2054
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PylRSAF expression cassette for constitutive expression via CMV 
       promoter


<220>
<221>  misc_feature
<222>  (1)..(588)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (690)..(2054)
<223>  CDS

<400>  44
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta      600

gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag      660

ctggctagcg tttaaactta agcttcacca tggataaaaa accgctgaat accctgatct      720

ctgctactgg tctgtggatg agtcgtaccg gaaccattca taaaatcaaa caccacgagg      780

ttagccgttc gaaaatctat attgagatgg cgtgtggcga tcatctggtt gtgaacaata      840

gccgctcttc tcgtacagca cgtgcactgc gtcaccacaa atatcgtaaa acctgtaaac      900

gttgccgtgt gtccgatgag gatctgaaca aattcctgac aaaagccaat gaggaccaaa      960

caagcgtgaa agtgaaagtc gttagcgctc ctacccgtac taaaaaagca atgccgaaat     1020

ccgttgctcg tgcccctaaa ccactggaaa acactgaagc agcacaggca cagccgtctg     1080

gaagcaaatt ctctccggcc attcctgttt ctacccagga gtccgtttct gttccagcaa     1140

gtgtgagcac cagcattagc agtattagca ccggtgccac cgctagcgcc ctggttaaag     1200

gcaataccaa tccgattaca agcatgtctg ccccggttca agcatcagct ccagcactga     1260

caaaatccca aaccgatcgt ctggaggttc tgctgaatcc gaaagacgaa atcagcctga     1320

attccggcaa accgtttcgt gaactggaga gcgaactgct gtcacgtcgt aaaaaagacc     1380

tgcaacaaat ctatgccgaa gaacgtgaga actatctggg gaaactggaa cgtgaaatca     1440

cccgcttttt cgtggatcgt ggctttctgg agatcaaatc cccgattctg attcctctgg     1500

agtatatcga gcgtatgggc atcgacaatg ataccgaact gagcaaacaa attttccgtg     1560

tggataaaaa cttctgtctg cgccctatgc tagcaccaaa tctggctaac tatctgcgca     1620

aactggaccg tgccctgcct gatcctatca aaatcttcga gatcggcccg tgttatcgta     1680

aagagtccga cggtaaagaa catctggagg agtttaccat gctgaacttt tgccaaatgg     1740

gttcaggttg tactcgtgag aacctggaaa gcatcatcac cgattttctg aaccacctgg     1800

gcattgactt caaaattgtg ggcgacagct gtatggtgtt tggcgacacc ctggatgtca     1860

tgcacggcga cctggaactg tctagtgccg ttgtgggccc aatcccgctg gatcgtgagt     1920

ggggtatcga caaaccttgg atcggtgcgg gttttggtct ggagcgtctg ctgaaagtaa     1980

aacacgactt caagaacatc aaacgtgctg cacgttccga gtcctattac aatggtattt     2040

ctactaacct gtaa                                                       2054


<210>  45
<211>  2105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NES-PylRSAF expression cassette for constitutive expression via 
       CMV promoter


<220>
<221>  misc_feature
<222>  (1)..(588)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (696)..(743)
<223>  NES

<220>
<221>  misc_feature
<222>  (744)..(2105)
<223>  CDS

<400>  45
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta      600

gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag      660

ctggctagcg tttaaactta agcttcacca tggcgtgccc ggtgccgctg cagctgccgc      720

cgctggaacg cctgaccctg gatgataaaa aaccgctgaa taccctgatc tctgctactg      780

gtctgtggat gagtcgtacc ggaaccattc ataaaatcaa acaccacgag gttagccgtt      840

cgaaaatcta tattgagatg gcgtgtggcg atcatctggt tgtgaacaat agccgctctt      900

ctcgtacagc acgtgcactg cgtcaccaca aatatcgtaa aacctgtaaa cgttgccgtg      960

tgtccgatga ggatctgaac aaattcctga caaaagccaa tgaggaccaa acaagcgtga     1020

aagtgaaagt cgttagcgct cctacccgta ctaaaaaagc aatgccgaaa tccgttgctc     1080

gtgcccctaa accactggaa aacactgaag cagcacaggc acagccgtct ggaagcaaat     1140

tctctccggc cattcctgtt tctacccagg agtccgtttc tgttccagca agtgtgagca     1200

ccagcattag cagtattagc accggtgcca ccgctagcgc cctggttaaa ggcaatacca     1260

atccgattac aagcatgtct gccccggttc aagcatcagc tccagcactg acaaaatccc     1320

aaaccgatcg tctggaggtt ctgctgaatc cgaaagacga aatcagcctg aattccggca     1380

aaccgtttcg tgaactggag agcgaactgc tgtcacgtcg taaaaaagac ctgcaacaaa     1440

tctatgccga agaacgtgag aactatctgg ggaaactgga acgtgaaatc acccgctttt     1500

tcgtggatcg tggctttctg gagatcaaat ccccgattct gattcctctg gagtatatcg     1560

agcgtatggg catcgacaat gataccgaac tgagcaaaca aattttccgt gtggataaaa     1620

acttctgtct gcgccctatg ctagcaccaa atctggctaa ctatctgcgc aaactggacc     1680

gtgccctgcc tgatcctatc aaaatcttcg agatcggccc gtgttatcgt aaagagtccg     1740

acggtaaaga acatctggag gagtttacca tgctgaactt ttgccaaatg ggttcaggtt     1800

gtactcgtga gaacctggaa agcatcatca ccgattttct gaaccacctg ggcattgact     1860

tcaaaattgt gggcgacagc tgtatggtgt ttggcgacac cctggatgtc atgcacggcg     1920

acctggaact gtctagtgcc gttgtgggcc caatcccgct ggatcgtgag tggggtatcg     1980

acaaaccttg gatcggtgcg ggttttggtc tggagcgtct gctgaaagta aaacacgact     2040

tcaagaacat caaacgtgct gcacgttccg agtcctatta caatggtatt tctactaacc     2100

tgtaa                                                                 2105


<210>  46
<211>  2194
<212>  DNA
<213>  NES-PylRSAF expression cassette containing " T-REx" promoter comprising two tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(584)
<223>  promoter

<220>
<221>  misc_feature
<222>  (586)..(604)
<223>  tetO

<220>
<221>  misc_feature
<222>  (607)..(625)
<223>  tetO

<220>
<221>  misc_feature
<222>  (785)..(832)
<223>  NES

<220>
<221>  misc_feature
<222>  (833)..(2194)
<223>  CDS

<400>  46
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata      600

gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg      660

cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc      720

tccggactct agcgtttaaa catcacaagt ttgtacaaaa aagcaggcgc tagacaccat      780

ggcgtgcccg gtgccgctgc agctgccgcc gctggaacgc ctgaccctgg atgataaaaa      840

accgctgaat accctgatct ctgctactgg tctgtggatg agtcgtaccg gaaccattca      900

taaaatcaaa caccacgagg ttagccgttc gaaaatctat attgagatgg cgtgtggcga      960

tcatctggtt gtgaacaata gccgctcttc tcgtacagca cgtgcactgc gtcaccacaa     1020

atatcgtaaa acctgtaaac gttgccgtgt gtccgatgag gatctgaaca aattcctgac     1080

aaaagccaat gaggaccaaa caagcgtgaa agtgaaagtc gttagcgctc ctacccgtac     1140

taaaaaagca atgccgaaat ccgttgctcg tgcccctaaa ccactggaaa acactgaagc     1200

agcacaggca cagccgtctg gaagcaaatt ctctccggcc attcctgttt ctacccagga     1260

gtccgtttct gttccagcaa gtgtgagcac cagcattagc agtattagca ccggtgccac     1320

cgctagcgcc ctggttaaag gcaataccaa tccgattaca agcatgtctg ccccggttca     1380

agcatcagct ccagcactga caaaatccca aaccgatcgt ctggaggttc tgctgaatcc     1440

gaaagacgaa atcagcctga attccggcaa accgtttcgt gaactggaga gcgaactgct     1500

gtcacgtcgt aaaaaagacc tgcaacaaat ctatgccgaa gaacgtgaga actatctggg     1560

gaaactggaa cgtgaaatca cccgcttttt cgtggatcgt ggctttctgg agatcaaatc     1620

cccgattctg attcctctgg agtatatcga gcgtatgggc atcgacaatg ataccgaact     1680

gagcaaacaa attttccgtg tggataaaaa cttctgtctg cgccctatgc tagcaccaaa     1740

tctggctaac tatctgcgca aactggaccg tgccctgcct gatcctatca aaatcttcga     1800

gatcggcccg tgttatcgta aagagtccga cggtaaagaa catctggagg agtttaccat     1860

gctgaacttt tgccaaatgg gttcaggttg tactcgtgag aacctggaaa gcatcatcac     1920

cgattttctg aaccacctgg gcattgactt caaaattgtg ggcgacagct gtatggtgtt     1980

tggcgacacc ctggatgtca tgcacggcga cctggaactg tctagtgccg ttgtgggccc     2040

aatcccgctg gatcgtgagt ggggtatcga caaaccttgg atcggtgcgg gttttggtct     2100

ggagcgtctg ctgaaagtaa aacacgactt caagaacatc aaacgtgctg cacgttccga     2160

gtcctattac aatggtattt ctactaacct gtaa                                 2194


<210>  47
<211>  1883
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NES-PylRSAF expression cassette containing "Tet-On" promoter 
       comprising 8 tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(458)
<223>  promoter

<220>
<221>  misc_feature
<222>  (18)..(36)
<223>  tetO

<220>
<221>  misc_feature
<222>  (54)..(72)
<223>  tetO

<220>
<221>  misc_feature
<222>  (90)..(108)
<223>  tetO

<220>
<221>  misc_feature
<222>  (126)..(144)
<223>  tetO

<220>
<221>  misc_feature
<222>  (162)..(180)
<223>  tetO

<220>
<221>  misc_feature
<222>  (198)..(216)
<223>  tetO

<220>
<221>  misc_feature
<222>  (234)..(252)
<223>  tetO

<220>
<221>  misc_feature
<222>  (270)..(288)
<223>  tetO

<220>
<221>  misc_feature
<222>  (474)..(521)
<223>  NES

<220>
<221>  misc_feature
<222>  (522)..(1883)
<223>  CDS

<400>  47
caattgctcg agtttactcc ctatcagtga tagagaacgt atgtcgactt tactccctat       60

cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac      120

tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga      180

acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat      240

cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatacgctt      300

taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc      360

tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg      420

aaagtccata tggctagcct gcagggatcc agatatcaag cttcaccatg gcgtgcccgg      480

tgccgctgca gctgccgccg ctggaacgcc tgaccctgga tgataaaaaa ccgctgaata      540

ccctgatctc tgctactggt ctgtggatga gtcgtaccgg aaccattcat aaaatcaaac      600

accacgaggt tagccgttcg aaaatctata ttgagatggc gtgtggcgat catctggttg      660

tgaacaatag ccgctcttct cgtacagcac gtgcactgcg tcaccacaaa tatcgtaaaa      720

cctgtaaacg ttgccgtgtg tccgatgagg atctgaacaa attcctgaca aaagccaatg      780

aggaccaaac aagcgtgaaa gtgaaagtcg ttagcgctcc tacccgtact aaaaaagcaa      840

tgccgaaatc cgttgctcgt gcccctaaac cactggaaaa cactgaagca gcacaggcac      900

agccgtctgg aagcaaattc tctccggcca ttcctgtttc tacccaggag tccgtttctg      960

ttccagcaag tgtgagcacc agcattagca gtattagcac cggtgccacc gctagcgccc     1020

tggttaaagg caataccaat ccgattacaa gcatgtctgc cccggttcaa gcatcagctc     1080

cagcactgac aaaatcccaa accgatcgtc tggaggttct gctgaatccg aaagacgaaa     1140

tcagcctgaa ttccggcaaa ccgtttcgtg aactggagag cgaactgctg tcacgtcgta     1200

aaaaagacct gcaacaaatc tatgccgaag aacgtgagaa ctatctgggg aaactggaac     1260

gtgaaatcac ccgctttttc gtggatcgtg gctttctgga gatcaaatcc ccgattctga     1320

ttcctctgga gtatatcgag cgtatgggca tcgacaatga taccgaactg agcaaacaaa     1380

ttttccgtgt ggataaaaac ttctgtctgc gccctatgct agcaccaaat ctggctaact     1440

atctgcgcaa actggaccgt gccctgcctg atcctatcaa aatcttcgag atcggcccgt     1500

gttatcgtaa agagtccgac ggtaaagaac atctggagga gtttaccatg ctgaactttt     1560

gccaaatggg ttcaggttgt actcgtgaga acctggaaag catcatcacc gattttctga     1620

accacctggg cattgacttc aaaattgtgg gcgacagctg tatggtgttt ggcgacaccc     1680

tggatgtcat gcacggcgac ctggaactgt ctagtgccgt tgtgggccca atcccgctgg     1740

atcgtgagtg gggtatcgac aaaccttgga tcggtgcggg ttttggtctg gagcgtctgc     1800

tgaaagtaaa acacgacttc aagaacatca aacgtgctgc acgttccgag tcctattaca     1860

atggtatttc tactaacctg taa                                             1883


<210>  48
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNAPyl expression cassette for expression via T7 promoter


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  T7 promoter

<220>
<221>  misc_feature
<222>  (18)..(86)
<223>  tRNA(Pyl)

<220>
<221>  misc_feature
<222>  (98)..(226)
<223>  T7term

<400>  48
taatacgact cactatagga aacctgatca tgtagatcga atggactcta aatccgttca       60

gccgggttag attcccgggg tttccgtttt tggccgcctg ctaacaaagc ccgaaaggaa      120

gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa      180

cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat                     226


<210>  49
<211>  295
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNAPyl-HDV ribozyme expression cassette for expression via T7 
       promoter


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  T7 promoter

<220>
<221>  misc_feature
<222>  (18)..(86)
<223>  tRNA(Pyl)

<220>
<221>  misc_feature
<222>  (87)..(154)
<223>  HDV

<220>
<221>  misc_feature
<222>  (167)..(295)
<223>  T7term

<400>  49
taatacgact cactatagga aacctgatca tgtagatcga atggactcta aatccgttca       60

gccgggttag attcccgggg tttccggcta gccatggtcc cagcctcctc gctggcggct      120

agtgggcaac atgcttcggc atggcgaatg ggactttaaa ctcgagctgc taacaaagcc      180

cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg      240

gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat           295


<210>  50
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNAPyl expression cassette containing a variant of the human U6 
       promoter modified to comprise 8 tetO before and two tetO 
       sequences after the U6 promoter


<220>
<221>  misc_feature
<222>  (24)..(42)
<223>  tetO

<220>
<221>  misc_feature
<222>  (60)..(78)
<223>  tetO

<220>
<221>  misc_feature
<222>  (96)..(114)
<223>  tetO

<220>
<221>  misc_feature
<222>  (132)..(150)
<223>  tetO

<220>
<221>  misc_feature
<222>  (168)..(186)
<223>  tetO

<220>
<221>  misc_feature
<222>  (204)..(222)
<223>  tetO

<220>
<221>  misc_feature
<222>  (240)..(285)
<223>  tetO

<220>
<221>  misc_feature
<222>  (276)..(294)
<223>  tetO

<220>
<221>  misc_feature
<222>  (337)..(537)
<223>  U6 promoter

<220>
<221>  misc_feature
<222>  (540)..(558)
<223>  tetO

<220>
<221>  misc_feature
<222>  (568)..(586)
<223>  tetO

<220>
<221>  misc_feature
<222>  (589)..(657)
<223>  tRNA(Pyl)

<400>  50
gaattccaat tgctcgagtt tactccctat cagtgataga gaacgtatgt cgactttact       60

ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat      120

gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga      180

tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact      240

ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat      300

gtcgaggtag gcgtgtacgg tcaaggtcgg gcaggaagag ggcctatttc ccatgattcc      360

ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt      420

aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt      480

tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagtt      540

ccctatcagt gatagagatt atatatctcc ctatcagtga tagagaccgg aaacctgatc      600

atgtagatcg aatggactct aaatccgttc agccgggtta gattcccggg gtttccgttt      660

tttggatcc                                                              669


<210>  51
<211>  178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNAPyl expression cassette containing a variant of the human H1 
       promoter modified to comprise 2 tetO sequences


<220>
<221>  misc_feature
<222>  (1)..(99)
<223>  H1 promoter

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  tetO

<220>
<221>  misc_feature
<222>  (77)..(95)
<223>  tetO

<220>
<221>  misc_feature
<222>  (100)..(168)
<223>  tRNA(Pyl)

<400>  51
atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatc cctatcagtg       60

atagagactt ataagttccc tatcagtgat agagatcccg gaaacctgat catgtagatc      120

gaatggactc taaatccgtt cagccgggtt agattcccgg ggtttccgaa gctttttt        178


<210>  52
<211>  248
<212>  PRT
<213>  TetOn3G protein


<220>
<221>  MISC_FEATURE
<223>  TetOn3G protein

<400>  52

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Gly Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Pro Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Ser Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Tyr Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Glu Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Lys Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp Arg Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Gly Pro 
        195                 200                 205             


Thr Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Pro Ala Asp Ala 
    210                 215                 220                 


Leu Asp Asp Phe Asp Leu Asp Met Leu Pro Ala Asp Ala Leu Asp Asp 
225                 230                 235                 240 


Phe Asp Leu Asp Met Leu Pro Gly 
                245             


<210>  53
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS Y306A, Y384F mutant (PylRSAF)_amino 
       acid sequence

<400>  53

Met Ala Cys Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr 
1               5                   10                  15      


Leu Asp Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu 
            20                  25                  30          


Trp Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val 
        35                  40                  45              


Ser Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val 
    50                  55                  60                  


Val Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His 
65                  70                  75                  80  


Lys Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu 
                85                  90                  95      


Asn Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val 
            100                 105                 110         


Lys Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser 
        115                 120                 125             


Val Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala 
    130                 135                 140                 


Gln Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln 
145                 150                 155                 160 


Glu Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile 
                165                 170                 175     


Ser Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro 
            180                 185                 190         


Ile Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr 
        195                 200                 205             


Lys Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu 
    210                 215                 220                 


Ile Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu 
225                 230                 235                 240 


Leu Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg 
                245                 250                 255     


Glu Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val 
            260                 265                 270         


Asp Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu 
        275                 280                 285             


Tyr Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln 
    290                 295                 300                 


Ile Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro 
305                 310                 315                 320 


Asn Leu Ala Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro 
                325                 330                 335     


Ile Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly 
            340                 345                 350         


Lys Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly 
        355                 360                 365             


Ser Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu 
    370                 375                 380                 


Asn His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val 
385                 390                 395                 400 


Phe Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser 
                405                 410                 415     


Ala Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys 
            420                 425                 430         


Pro Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys 
        435                 440                 445             


His Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr 
    450                 455                 460                 


Asn Gly Ile Ser Thr Asn Leu 
465                 470     


<210>  54
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS N346A, C348A mutant (PylRSAA)_amino 
       acid sequence

<400>  54

Met Ala Cys Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr 
1               5                   10                  15      


Leu Asp Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu 
            20                  25                  30          


Trp Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val 
        35                  40                  45              


Ser Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val 
    50                  55                  60                  


Val Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His 
65                  70                  75                  80  


Lys Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu 
                85                  90                  95      


Asn Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val 
            100                 105                 110         


Lys Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser 
        115                 120                 125             


Val Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala 
    130                 135                 140                 


Gln Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln 
145                 150                 155                 160 


Glu Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile 
                165                 170                 175     


Ser Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro 
            180                 185                 190         


Ile Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr 
        195                 200                 205             


Lys Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu 
    210                 215                 220                 


Ile Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu 
225                 230                 235                 240 


Leu Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg 
                245                 250                 255     


Glu Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val 
            260                 265                 270         


Asp Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu 
        275                 280                 285             


Tyr Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln 
    290                 295                 300                 


Ile Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro 
305                 310                 315                 320 


Asn Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro 
                325                 330                 335     


Ile Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly 
            340                 345                 350         


Lys Glu His Leu Glu Glu Phe Thr Met Leu Ala Phe Ala Gln Met Gly 
        355                 360                 365             


Ser Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu 
    370                 375                 380                 


Asn His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val 
385                 390                 395                 400 


Tyr Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser 
                405                 410                 415     


Ala Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys 
            420                 425                 430         


Pro Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys 
        435                 440                 445             


His Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr 
    450                 455                 460                 


Asn Gly Ile Ser Thr Asn Leu 
465                 470     


<210>  55
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Methanosarcina mazei PylRS Y306A, N346A, C348A, Y384Fmutant 
       (PylRSAAAF)_amino acid sequence

<400>  55

Ala Cys Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu 
1               5                   10                  15      


Asp Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
            20                  25                  30          


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
        35                  40                  45              


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
    50                  55                  60                  


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
65                  70                  75                  80  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
                85                  90                  95      


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
            100                 105                 110         


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
        115                 120                 125             


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
    130                 135                 140                 


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
145                 150                 155                 160 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
                165                 170                 175     


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
            180                 185                 190         


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
        195                 200                 205             


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
    210                 215                 220                 


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
225                 230                 235                 240 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
                245                 250                 255     


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
            260                 265                 270         


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
        275                 280                 285             


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
    290                 295                 300                 


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
305                 310                 315                 320 


Leu Ala Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
                325                 330                 335     


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
            340                 345                 350         


Glu His Leu Glu Glu Phe Thr Met Leu Ala Phe Ala Gln Met Gly Ser 
        355                 360                 365             


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
    370                 375                 380                 


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Phe 
385                 390                 395                 400 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
                405                 410                 415     


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
            420                 425                 430         


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
        435                 440                 445             


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
    450                 455                 460                 


Gly Ile Ser Thr Asn Leu 
465                 470 


<210>  56
<211>  4437
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCAGGS-T7-RNAP


<220>
<221>  misc_feature
<222>  (1)..(380)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (382)..(659)
<223>  Chicken b-actin promoter

<220>
<221>  misc_feature
<222>  (661)..(1678)
<223>  Chimeric intron chicken actin

<220>
<221>  misc_feature
<222>  (1789)..(4437)
<223>  T7-RNAP

<400>  56
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct      420

cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga      480

tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg      540

gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc      600

cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg      660

ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc      720

gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc      780

tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga      840

aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg      900

cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg      960

ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg     1020

gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt     1080

gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca     1140

cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg     1200

tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc     1260

ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg     1320

cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc     1380

gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac     1440

cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga     1500

gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc     1560

gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt     1620

gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct     1680

cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgagctcat     1740

cggatccaga tctgatccgg atttactaac tggaagaggc actaaatgaa cacgattaac     1800

atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa cactctggct     1860

gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga gtcttacgag     1920

atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg     1980

gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat tgcacgcatc     2040

aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc cttccagttc     2100

ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac tctggcttgc     2160

ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt     2220

gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt caagaaaaac     2280

gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt     2340

gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat     2400

aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga gtcaaccgga     2460

atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga gactatcgaa     2520

ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc tggcatctct     2580

ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac tggtggtggc     2640

tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa gaaagcactg     2700

atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat tgcgcaaaac     2760

accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac caagtggaag     2820

cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat gaaaccggaa     2880

gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc tgctgtgtac     2940

cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct tgagcaagcc     3000

aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg gcgcggtcgt     3060

gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg actgcttacg     3120

ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat ccacggtgca     3180

aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat tgaggaaaac     3240

cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg ggctgagcaa     3300

gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca gcaccacggc     3360

ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg catccagcac     3420

ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct tcctagtgaa     3480

accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct acaagcagac     3540

gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac tggtgaaatc     3600

tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc ttacggtgtt     3660

actcgcagtg tgactaagcg ttcagtcatg acgctggctt acgggtccaa agagttcggc     3720

ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg caagggtctg     3780

atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg ggaatctgtg     3840

agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc tgctaagctg     3900

ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg ttgcgctgtg     3960

cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc tattcagacg     4020

cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa caccaacaaa     4080

gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt tgtacacagc     4140

caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta cggaatcgaa     4200

tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc gaacctgttc     4260

aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact ggctgatttc     4320

tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc agcacttccg     4380

gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt cgcgtaa        4437


<210>  57
<211>  515
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(515)
<223>  dsRNA-binding polypeptide  (uniprot: Q03963)

<400>  57

Met Ala Ser Asp Thr Pro Gly Phe Tyr Met Asp Lys Leu Asn Lys Tyr 
1               5                   10                  15      


Arg Gln Met His Gly Val Ala Ile Thr Tyr Lys Glu Leu Ser Thr Ser 
            20                  25                  30          


Gly Pro Pro His Asp Arg Arg Phe Thr Phe Gln Val Leu Ile Asp Glu 
        35                  40                  45              


Lys Glu Phe Pro Glu Ala Lys Gly Arg Ser Lys Gln Glu Ala Arg Asn 
    50                  55                  60                  


Ala Ala Ala Lys Leu Ala Val Asp Ile Leu Asp Asn Glu Asn Lys Val 
65                  70                  75                  80  


Asp Cys His Thr Ser Ala Ser Glu Gln Gly Leu Phe Val Gly Asn Tyr 
                85                  90                  95      


Ile Gly Leu Val Asn Ser Phe Ala Gln Lys Lys Lys Leu Ser Val Asn 
            100                 105                 110         


Tyr Glu Gln Cys Glu Pro Asn Ser Glu Leu Pro Gln Arg Phe Ile Cys 
        115                 120                 125             


Lys Cys Lys Ile Gly Gln Thr Met Tyr Gly Thr Gly Ser Gly Val Thr 
    130                 135                 140                 


Lys Gln Glu Ala Lys Gln Leu Ala Ala Lys Glu Ala Tyr Gln Lys Leu 
145                 150                 155                 160 


Leu Lys Ser Pro Pro Lys Thr Ala Gly Thr Ser Ser Ser Val Val Thr 
                165                 170                 175     


Ser Thr Phe Ser Gly Phe Ser Ser Ser Ser Ser Met Thr Ser Asn Gly 
            180                 185                 190         


Val Ser Gln Ser Ala Pro Gly Ser Phe Ser Ser Glu Asn Val Phe Thr 
        195                 200                 205             


Asn Gly Leu Gly Glu Asn Lys Arg Lys Ser Gly Val Lys Val Ser Pro 
    210                 215                 220                 


Asp Asp Val Gln Arg Asn Lys Tyr Thr Leu Asp Ala Arg Phe Asn Ser 
225                 230                 235                 240 


Asp Phe Glu Asp Ile Glu Glu Ile Gly Leu Gly Gly Phe Gly Gln Val 
                245                 250                 255     


Phe Lys Ala Lys His Arg Ile Asp Gly Lys Arg Tyr Ala Ile Lys Arg 
            260                 265                 270         


Val Lys Tyr Asn Thr Glu Lys Ala Glu His Glu Val Gln Ala Leu Ala 
        275                 280                 285             


Glu Leu Asn His Val Asn Ile Val Gln Tyr His Ser Cys Trp Glu Gly 
    290                 295                 300                 


Val Asp Tyr Asp Pro Glu His Ser Met Ser Asp Thr Ser Arg Tyr Lys 
305                 310                 315                 320 


Thr Arg Cys Leu Phe Ile Gln Met Glu Phe Cys Asp Lys Gly Thr Leu 
                325                 330                 335     


Glu Gln Trp Met Arg Asn Arg Asn Gln Ser Lys Val Asp Lys Ala Leu 
            340                 345                 350         


Ile Leu Asp Leu Tyr Glu Gln Ile Val Thr Gly Val Glu Tyr Ile His 
        355                 360                 365             


Ser Lys Gly Leu Ile His Arg Asp Leu Lys Pro Gly Asn Ile Phe Leu 
    370                 375                 380                 


Val Asp Glu Arg His Ile Lys Ile Gly Asp Phe Gly Leu Ala Thr Ala 
385                 390                 395                 400 


Leu Glu Asn Asp Gly Lys Ser Arg Thr Arg Arg Thr Gly Thr Leu Gln 
                405                 410                 415     


Tyr Met Ser Pro Glu Gln Leu Phe Leu Lys His Tyr Gly Lys Glu Val 
            420                 425                 430         


Asp Ile Phe Ala Leu Gly Leu Ile Leu Ala Glu Leu Leu His Thr Cys 
        435                 440                 445             


Phe Thr Glu Ser Glu Lys Ile Lys Phe Phe Glu Ser Leu Arg Lys Gly 
    450                 455                 460                 


Asp Phe Ser Asn Asp Ile Phe Asp Asn Lys Glu Lys Ser Leu Leu Lys 
465                 470                 475                 480 


Lys Leu Leu Ser Glu Lys Pro Lys Asp Arg Pro Glu Thr Ser Glu Ile 
                485                 490                 495     


Leu Lys Thr Leu Ala Glu Trp Arg Asn Ile Ser Glu Lys Lys Lys Arg 
            500                 505                 510         


Asn Thr Cys 
        515 


<210>  58
<211>  552
<212>  PRT
<213>  Pan troglodytes


<220>
<221>  MISC_FEATURE
<222>  (1)..(552)
<223>  dsRNA-binding polypeptide  (uniprot: B9UKH7)

<400>  58

Met Ala Gly Asp Leu Ser Ala Gly Phe Phe Met Glu Glu Leu Asn Thr 
1               5                   10                  15      


Tyr Arg Gln Lys Gln Gly Val Val Leu Lys Tyr Gln Glu Leu Pro Asn 
            20                  25                  30          


Ser Gly Pro Pro His Asp Arg Arg Phe Thr Phe Arg Val Ile Ile Asp 
        35                  40                  45              


Gly Arg Glu Phe Pro Glu Gly Glu Gly Arg Ser Lys Lys Glu Ala Lys 
    50                  55                  60                  


Asn Ala Ala Ala Lys Leu Ala Val Glu Ile Leu Asn Lys Glu Lys Lys 
65                  70                  75                  80  


Ala Val Ser Pro Leu Val Leu Thr Thr Thr Asn Ser Ser Glu Gly Leu 
                85                  90                  95      


Ser Met Gly Asn Tyr Ile Gly Leu Ile Asn Arg Ile Ala Gln Lys Lys 
            100                 105                 110         


Arg Leu Thr Val Asn Tyr Glu Gln Cys Ala Ser Gly Val His Gly Pro 
        115                 120                 125             


Glu Gly Phe His Tyr Lys Cys Lys Met Gly Gln Lys Glu Tyr Ser Ile 
    130                 135                 140                 


Gly Thr Ser Ser Thr Lys Gln Glu Ala Lys Gln Leu Ala Ala Lys Leu 
145                 150                 155                 160 


Ala Tyr Leu Gln Ile Leu Ser Glu Glu Thr Ser Val Lys Ser Asp Tyr 
                165                 170                 175     


Leu Ser Ser Gly Ser Phe Ala Thr Thr Cys Asp Ser Gln Ser Asn Ser 
            180                 185                 190         


Leu Val Thr Ser Thr Leu Ala Ser Glu Ser Ser Ser Glu Gly Asp Phe 
        195                 200                 205             


Ser Ala Asp Thr Ser Glu Ile Asn Ser Asn Ser Asp Ser Leu Asn Ser 
    210                 215                 220                 


Ser Ser Leu Leu Met Asn Gly Leu Arg Asn Asn Gln Arg Lys Ala Lys 
225                 230                 235                 240 


Arg Ser Leu Ala Pro Arg Phe Asp Leu Pro Asp Met Lys Glu Thr Lys 
                245                 250                 255     


Tyr Thr Val Asp Lys Arg Phe Gly Met Asp Phe Lys Glu Ile Glu Leu 
            260                 265                 270         


Ile Gly Ser Gly Gly Phe Gly Gln Val Phe Lys Ala Lys His Arg Ile 
        275                 280                 285             


Asp Gly Lys Thr Tyr Val Ile Lys Arg Val Lys Tyr Asn Asn Glu Lys 
    290                 295                 300                 


Ala Glu Arg Glu Val Lys Ala Leu Ala Lys Leu Asp His Val Asn Ile 
305                 310                 315                 320 


Val His Tyr Asn Gly Cys Trp Asp Gly Phe Asp Tyr Asp Pro Glu Thr 
                325                 330                 335     


Ser Asp Asp Asp Leu Glu Ser Ser Asp Tyr Asp Pro Glu Asn Ser Lys 
            340                 345                 350         


Asn Ser Ser Arg Ser Lys Thr Lys Cys Leu Phe Ile Gln Met Glu Phe 
        355                 360                 365             


Cys Asp Lys Gly Thr Leu Glu Gln Trp Ile Glu Asp Arg Arg Gly Lys 
    370                 375                 380                 


Lys Leu Asp Lys Val Leu Ala Leu Glu Leu Phe Glu Gln Ile Thr Lys 
385                 390                 395                 400 


Gly Val Asp Tyr Ile His Ser Lys Lys Leu Ile His Arg Asp Leu Lys 
                405                 410                 415     


Pro Ser Asn Ile Phe Leu Val Asp Thr Lys Gln Val Lys Ile Gly Asp 
            420                 425                 430         


Phe Gly Leu Val Thr Ser Leu Lys Asn Asp Gly Lys Arg Thr Arg Ser 
        435                 440                 445             


Lys Gly Thr Leu Arg Tyr Met Ser Pro Glu Gln Ile Ser Ser Gln Asp 
    450                 455                 460                 


Tyr Gly Lys Glu Val Asp Leu Tyr Ala Leu Gly Leu Ile Leu Ala Glu 
465                 470                 475                 480 


Leu Leu His Val Cys Asp Thr Ala Phe Glu Thr Ser Lys Phe Phe Thr 
                485                 490                 495     


Asp Leu Arg Asp Gly Ile Ile Ser Asp Ile Leu Phe Asp Lys Lys Glu 
            500                 505                 510         


Lys Thr Leu Leu Gln Lys Leu Leu Ser Lys Lys Pro Glu Asp Arg Pro 
        515                 520                 525             


Asn Thr Ser Glu Ile Leu Arg Thr Leu Thr Val Trp Lys Lys Ser Pro 
    530                 535                 540                 


Glu Lys Asn Glu Arg His Thr Cys 
545                 550         


<210>  59
<211>  551
<212>  PRT
<213>  Gorilla gorilla


<220>
<221>  MISC_FEATURE
<222>  (1)..(551)
<223>  dsRNA-binding polypeptide (uniprot: G3RTN4)

<400>  59

Met Ala Gly Asp Leu Ser Ala Gly Phe Phe Met Glu Glu Leu Asn Thr 
1               5                   10                  15      


Tyr Arg Gln Lys Gln Gly Val Val Leu Lys Tyr Gln Glu Leu Pro Asn 
            20                  25                  30          


Ser Gly Pro Pro His Asp Arg Arg Phe Thr Phe Arg Val Ile Ile Asp 
        35                  40                  45              


Glu Arg Glu Phe Pro Glu Gly Glu Gly Arg Ser Lys Lys Glu Ala Lys 
    50                  55                  60                  


Asn Ala Ala Ala Lys Leu Ala Val Glu Ile Leu Asn Lys Glu Lys Lys 
65                  70                  75                  80  


Ala Val Ser Pro Leu Leu Leu Thr Thr Thr Asn Ser Ser Glu Gly Leu 
                85                  90                  95      


Ser Met Gly Asn Tyr Ile Gly Leu Ile Asn Arg Ile Ala Gln Lys Lys 
            100                 105                 110         


Arg Leu Thr Val Asn Tyr Glu Gln Cys Ala Ser Gly Val His Gly Pro 
        115                 120                 125             


Glu Gly Phe His Tyr Lys Cys Lys Met Gly Gln Lys Glu Tyr Ser Ile 
    130                 135                 140                 


Gly Thr Gly Ser Thr Lys Gln Glu Ala Lys Gln Leu Ala Ala Lys Leu 
145                 150                 155                 160 


Ala Tyr Leu Gln Ile Val Ser Glu Glu Thr Ser Val Lys Ser Asp Tyr 
                165                 170                 175     


Leu Ser Ser Gly Ser Phe Ala Thr Thr Cys Asp Ser Gln Ser Asn Ser 
            180                 185                 190         


Leu Val Thr Ser Thr Leu Ala Ser Glu Ser Ser Ser Glu Gly Asp Phe 
        195                 200                 205             


Ser Ala Asp Thr Ser Glu Ile Asn Ser Asn Ser Asp Ser Leu Asn Ser 
    210                 215                 220                 


Ser Ser Leu Leu Met Asn Gly Leu Arg Asn Asn Gln Arg Lys Ala Lys 
225                 230                 235                 240 


Arg Ser Leu Ala Pro Arg Phe Asp Leu Pro Asp Met Lys Glu Thr Lys 
                245                 250                 255     


Tyr Thr Val Asp Lys Arg Phe Gly Met Asp Phe Lys Glu Ile Glu Leu 
            260                 265                 270         


Ile Gly Ser Gly Gly Phe Gly Gln Val Phe Lys Ala Lys His Arg Ile 
        275                 280                 285             


Asp Gly Lys Thr Tyr Val Ile Lys Arg Val Lys Tyr Asn Asn Glu Lys 
    290                 295                 300                 


Ala Glu Arg Glu Val Lys Ala Leu Ala Lys Leu Asp His Val Asn Ile 
305                 310                 315                 320 


Val His Tyr Asn Gly Cys Trp Asp Gly Val Asp Tyr Asp Pro Glu Thr 
                325                 330                 335     


Ser Asp Asp Tyr Leu Glu Ser Ser Asp Tyr Asp Pro Glu Asn Ser Lys 
            340                 345                 350         


Asn Ser Ser Arg Ser Lys Thr Lys Cys Leu Phe Ile Gln Met Glu Phe 
        355                 360                 365             


Cys Asp Lys Gly Thr Leu Glu Gln Trp Ile Glu Asn Arg Arg Gly Glu 
    370                 375                 380                 


Lys Leu Asp Lys Val Leu Ala Leu Glu Leu Phe Glu Gln Ile Thr Lys 
385                 390                 395                 400 


Gly Val Asp Tyr Ile His Ser Lys Lys Leu Ile His Arg Asp Leu Lys 
                405                 410                 415     


Pro Ser Asn Ile Phe Leu Val Asp Thr Lys Gln Val Lys Ile Gly Asp 
            420                 425                 430         


Phe Gly Leu Val Thr Ser Leu Lys Asn Asp Gly Lys Arg Thr Arg Ser 
        435                 440                 445             


Lys Gly Thr Leu Arg Tyr Met Ser Pro Glu Gln Ile Ser Ser Gln Asp 
    450                 455                 460                 


Tyr Gly Lys Glu Val Asp Leu Tyr Ala Leu Gly Leu Ile Leu Ala Glu 
465                 470                 475                 480 


Leu Leu His Val Cys Asp Thr Ala Phe Glu Thr Ser Lys Phe Phe Thr 
                485                 490                 495     


Asp Leu Arg Asp Gly Ile Ile Ser Asp Ile Phe Asp Lys Lys Glu Lys 
            500                 505                 510         


Thr Leu Leu Gln Lys Leu Leu Ser Lys Lys Pro Glu Asp Arg Pro Asn 
        515                 520                 525             


Thr Ser Glu Ile Leu Arg Thr Leu Thr Val Trp Lys Lys Ser Pro Glu 
    530                 535                 540                 


Lys Asn Glu Arg His Thr Cys 
545                 550     


<210>  60
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA of Methanosarcina mazei for unidentified codon 

       (tRNAPyl, nnn)


<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  anticodon

<400>  60
ggaaacctga tcatgtagat cgaatggact nnnaatccgt tcagccgggt tagattcccg       60

gggtttccg                                                               69


