                         SEQUENCE LISTING

<110>  University of Washington
       Consejo Superior de Investigaciones Cientificas
       Mougous, Joseph
       Peterson, Snow Brook
       Ting, See-Yeun
       Martinez, Esteban
       de Lorenzo, Victor
 
<120>  TARGETED DEPLETION OF BACTERIA FROM MIXED POPULATIONS THROUGH 
       PROGRAMMABLE CELL-CELL ADHESION

<130>  3915-P903WO / UWOTL-0073528

<150>  US 62/976,885
<151>  2020-02-14

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  124
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Asp Asp Tyr 
            20                  25                  30          


Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ser Cys Ile Arg Arg Ser Asp Gly Met Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ser Asp Tyr Ser Asp Tyr Gly Leu Arg Pro Tyr Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  2
<211>  111
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Arg Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ile Ala Ala Gly Thr Leu Phe Ser Gly Lys 
            20                  25                  30          


Ala Val Gly Trp Tyr Arg Gln Ala Pro Gly Lys Asp Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Leu Ser Ser Gly Arg Glu Thr Tyr Tyr Ile Gly Pro Val Lys 
    50                  55                  60                  


Gly Arg Phe Ile Ile Ser Arg Asp Asp Asn Lys Asp Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Asn Leu Lys Leu Glu Asp Thr Ala Ile Tyr Thr Cys Arg 
                85                  90                  95      


Leu Gly Leu His Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
            100                 105                 110     


<210>  3
<211>  123
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  3

Gln Gly Gln Leu Val Glu Ser Gly Gly Gly Met Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Gly Trp 
            20                  25                  30          


Thr Ala Ala Trp Phe Arg Gln Ala Pro Gly Lys Asp Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Arg Ser Gly Asp Tyr Thr Tyr Tyr Thr Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Ser Ala Lys Asn Asn Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Thr Gly Thr Trp Ala Thr Met Asp Arg Arg Tyr Asp Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Arg Val Thr Val Ser Ser 
        115                 120             


<210>  4
<211>  124
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  4

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Pro Tyr Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Thr Pro Gly Asn Glu Cys Glu Leu Val 
        35                  40                  45              


Ser Thr Thr Gly Pro Glu Ser Asp Pro Ser Phe Trp Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Thr Gly Met Tyr Val 
                85                  90                  95      


Cys Ala Ser Glu Leu Gly Ala Gly Ser Gly Arg Cys Tyr Gly Tyr His 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  5
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  5
ttaacatatg cggattagaa aaac                                              24


<210>  6
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  6
ttaactgcag cggccgctac tagtattatt acagatcttc ctcgctgatc agcttctgtt       60

cgccaccgcc actactaacg gtaacttgcg                                        90


<210>  7
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  7
ttaactgcag cggccgctac tagtattatt acagatcttc ctcgctgatc agcttctgtt       60

cgccaccgcc gctactcacc gtaacctgtg                                        90


<210>  8
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  8
catgaattcc cgggagagct ccctcgtttg gcggtgtgg                              39


<210>  9
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  9
ttcaaaaatc attaagtaat cgtgttatcc tgccaggcag                             40


<210>  10
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  10
ctgcctggca ggataacacg attacttaat gatttttgaa                             40


<210>  11
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  11
cgacggatcc caagcttctt ctagatgaag cgacttgcag tttatgttg                   49


<210>  12
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  12
ctaagcggtg ctatcacggg caat                                              24


<210>  13
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  13
catccccttc acaaatctca taaaag                                            26


<210>  14
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  14
catgaattcc cgggagagct cgtcctgaga ggttccggaa taac                        44


<210>  15
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  15
ttcatcttta cttgtttctc cttgaaaaga agctc                                  35


<210>  16
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  16
gagaaacaag taaagatgaa atcggcccc                                         29


<210>  17
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  17
cgacggatcc caagcttctt ctagacatcg ctgaacagcg tgtc                        44


<210>  18
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  18
cagcgctggc tggaacatc                                                    19


<210>  19
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  19
caggtttgct ctctaccgg                                                    19


<210>  20
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  20
ttaacatatg cggattagaa aaac                                              24


<210>  21
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  21
taacaaccat gagtaatcat ctagtatttc tcctctttct                             40


<210>  22
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  22
agaaagagga gaaatactag atgattactc atggttgtta                             40


<210>  23
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  23
ttaactgcag cggccgctac tagtattatt attattctac acaaaccgca t                51


<210>  24
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  24
ttaacatatg cggattagaa aaac                                              24


<210>  25
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  25
ctttcaacca gctgaacttg tctagtcgca ccatcaaaaa atataac                     47


<210>  26
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  26
gttatatttt ttgatggtgc gactagacaa gttcagctgg ttgaaag                     47


<210>  27
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  27
ttaactgcag cggccgctac tagtattatt agctgctaac ggtaacttgg g                51


<210>  28
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  28
ttaacatatg cggattagaa aaac                                              24


<210>  29
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  29
ctttcaacca gctggccttg tctagtcgca ccatcaaaaa atataac                     47


<210>  30
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  30
gttatatttt ttgatggtgc gactagacaa ggccagctgg ttgaaag                     47


<210>  31
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  31
ttaactgcag cggccgctac tagtattatt agctgctaac ggtaacacgg                  50


<210>  32
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  32
tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc tgtaggctgg       60

agctgcttcg                                                              70


<210>  33
<211>  69
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  33
acccaagacg ggccgatcac cagtattttc atgcagagct cttatgcgtc catatgaata       60

tcctcctta                                                               69


<210>  34
<211>  810
<212>  PRT
<213>  Escherichia coli

<400>  34

Met Ala Met Lys Lys Leu Leu Ile Ala Ser Leu Leu Phe Ser Ser Ala 
1               5                   10                  15      


Thr Val Tyr Gly Ala Glu Gly Phe Val Val Lys Asp Ile His Phe Glu 
            20                  25                  30          


Gly Leu Gln Arg Val Ala Val Gly Ala Ala Leu Leu Ser Met Pro Val 
        35                  40                  45              


Arg Thr Gly Asp Thr Val Asn Asp Glu Asp Ile Ser Asn Thr Ile Arg 
    50                  55                  60                  


Ala Leu Phe Ala Thr Gly Asn Phe Glu Asp Val Arg Val Leu Arg Asp 
65                  70                  75                  80  


Gly Asp Thr Leu Leu Val Gln Val Lys Glu Arg Pro Thr Ile Ala Ser 
                85                  90                  95      


Ile Thr Phe Ser Gly Asn Lys Ser Val Lys Asp Asp Met Leu Lys Gln 
            100                 105                 110         


Asn Leu Glu Ala Ser Gly Val Arg Val Gly Glu Ser Leu Asp Arg Thr 
        115                 120                 125             


Thr Ile Ala Asp Ile Glu Lys Gly Leu Glu Asp Phe Tyr Tyr Ser Val 
    130                 135                 140                 


Gly Lys Tyr Ser Ala Ser Val Lys Ala Val Val Thr Pro Leu Pro Arg 
145                 150                 155                 160 


Asn Arg Val Asp Leu Lys Leu Val Phe Gln Glu Gly Val Ser Ala Glu 
                165                 170                 175     


Ile Gln Gln Ile Asn Ile Val Gly Asn His Ala Phe Thr Thr Asp Glu 
            180                 185                 190         


Leu Ile Ser His Phe Gln Leu Arg Asp Glu Val Pro Trp Trp Asn Val 
        195                 200                 205             


Val Gly Asp Arg Lys Tyr Gln Lys Gln Lys Leu Ala Gly Asp Leu Glu 
    210                 215                 220                 


Thr Leu Arg Ser Tyr Tyr Leu Asp Arg Gly Tyr Ala Arg Phe Asn Ile 
225                 230                 235                 240 


Asp Ser Thr Gln Val Ser Leu Thr Pro Asp Lys Lys Gly Ile Tyr Val 
                245                 250                 255     


Thr Val Asn Ile Thr Glu Gly Asp Gln Tyr Lys Leu Ser Gly Val Glu 
            260                 265                 270         


Val Ser Gly Asn Leu Ala Gly His Ser Ala Glu Ile Glu Gln Leu Thr 
        275                 280                 285             


Lys Ile Glu Pro Gly Glu Leu Tyr Asn Gly Thr Lys Val Thr Lys Met 
    290                 295                 300                 


Glu Asp Asp Ile Lys Lys Leu Leu Gly Arg Tyr Gly Tyr Ala Tyr Pro 
305                 310                 315                 320 


Arg Val Gln Ser Met Pro Glu Ile Asn Asp Ala Asp Lys Thr Val Lys 
                325                 330                 335     


Leu Arg Val Asn Val Asp Ala Gly Asn Arg Phe Tyr Val Arg Lys Ile 
            340                 345                 350         


Arg Phe Glu Gly Asn Asp Thr Ser Lys Asp Ala Val Leu Arg Arg Glu 
        355                 360                 365             


Met Arg Gln Met Glu Gly Ala Trp Leu Gly Ser Asp Leu Val Asp Gln 
    370                 375                 380                 


Gly Lys Glu Arg Leu Asn Arg Leu Gly Phe Phe Glu Thr Val Asp Thr 
385                 390                 395                 400 


Asp Thr Gln Arg Val Pro Gly Ser Pro Asp Gln Val Asp Val Val Tyr 
                405                 410                 415     


Lys Val Lys Glu Arg Asn Thr Gly Ser Phe Asn Phe Gly Ile Gly Tyr 
            420                 425                 430         


Gly Thr Glu Ser Gly Val Ser Phe Gln Ala Gly Val Gln Gln Asp Asn 
        435                 440                 445             


Trp Leu Gly Thr Gly Tyr Ala Val Gly Ile Asn Gly Thr Lys Asn Asp 
    450                 455                 460                 


Tyr Gln Thr Tyr Ala Glu Leu Ser Val Thr Asn Pro Tyr Phe Thr Val 
465                 470                 475                 480 


Asp Gly Val Ser Leu Gly Gly Arg Leu Phe Tyr Asn Asp Phe Gln Ala 
                485                 490                 495     


Asp Asp Ala Asp Leu Ser Asp Tyr Thr Asn Lys Ser Tyr Gly Thr Asp 
            500                 505                 510         


Val Thr Leu Gly Phe Pro Ile Asn Glu Tyr Asn Ser Leu Arg Ala Gly 
        515                 520                 525             


Leu Gly Tyr Val His Asn Ser Leu Ser Asn Met Gln Pro Gln Val Ala 
    530                 535                 540                 


Met Trp Arg Tyr Leu Tyr Ser Met Gly Glu His Pro Ser Thr Ser Asp 
545                 550                 555                 560 


Gln Asp Asn Ser Phe Lys Thr Asp Asp Phe Thr Phe Asn Tyr Gly Trp 
                565                 570                 575     


Thr Tyr Asn Lys Leu Asp Arg Gly Tyr Phe Pro Thr Asp Gly Ser Arg 
            580                 585                 590         


Val Asn Leu Thr Gly Lys Val Thr Ile Pro Gly Ser Asp Asn Glu Tyr 
        595                 600                 605             


Tyr Lys Val Thr Leu Asp Thr Ala Thr Tyr Val Pro Ile Asp Asp Asp 
    610                 615                 620                 


His Lys Trp Val Val Leu Gly Arg Thr Arg Trp Gly Tyr Gly Asp Gly 
625                 630                 635                 640 


Leu Gly Gly Lys Glu Met Pro Phe Tyr Glu Asn Phe Tyr Ala Gly Gly 
                645                 650                 655     


Ser Ser Thr Val Arg Gly Phe Gln Ser Asn Thr Ile Gly Pro Lys Ala 
            660                 665                 670         


Val Tyr Phe Pro His Gln Ala Ser Asn Tyr Asp Pro Asp Tyr Asp Tyr 
        675                 680                 685             


Glu Cys Ala Thr Gln Asp Gly Ala Lys Asp Leu Cys Lys Ser Asp Asp 
    690                 695                 700                 


Ala Val Gly Gly Asn Ala Met Ala Val Ala Ser Leu Glu Phe Ile Thr 
705                 710                 715                 720 


Pro Thr Pro Phe Ile Ser Asp Lys Tyr Ala Asn Ser Val Arg Thr Ser 
                725                 730                 735     


Phe Phe Trp Asp Met Gly Thr Val Trp Asp Thr Asn Trp Asp Ser Ser 
            740                 745                 750         


Gln Tyr Ser Gly Tyr Pro Asp Tyr Ser Asp Pro Ser Asn Ile Arg Met 
        755                 760                 765             


Ser Ala Gly Ile Ala Leu Gln Trp Met Ser Pro Leu Gly Pro Leu Val 
    770                 775                 780                 


Phe Ser Tyr Ala Gln Pro Phe Lys Lys Tyr Asp Gly Asp Lys Ala Glu 
785                 790                 795                 800 


Gln Phe Gln Phe Asn Ile Gly Lys Thr Trp 
                805                 810 


<210>  35
<211>  280
<212>  PRT
<213>  Escherichia coli

<400>  35

Gln Thr Lys Ala Ser Ile Thr Glu Ile Lys Ala Asp Lys Thr Thr Ala 
1               5                   10                  15      


Val Ala Asn Gly Lys Asp Ala Ile Lys Tyr Thr Val Lys Val Met Lys 
            20                  25                  30          


Asn Gly Gln Pro Val Asn Asn Gln Ser Val Thr Phe Ser Thr Asn Phe 
        35                  40                  45              


Gly Met Phe Asn Gly Lys Ser Gln Thr Gln Ala Thr Thr Gly Asn Asp 
    50                  55                  60                  


Gly Arg Ala Thr Ile Thr Leu Thr Ser Ser Ser Ala Gly Lys Ala Thr 
65                  70                  75                  80  


Val Ser Ala Thr Val Ser Asp Gly Ala Glu Val Lys Ala Thr Glu Val 
                85                  90                  95      


Thr Phe Phe Asp Glu Leu Lys Ile Asp Asn Lys Val Asp Ile Ile Gly 
            100                 105                 110         


Asn Asn Val Arg Gly Glu Leu Pro Asn Ile Trp Leu Gln Tyr Gly Gln 
        115                 120                 125             


Phe Lys Leu Lys Ala Ser Gly Gly Asp Gly Thr Tyr Ser Trp Tyr Ser 
    130                 135                 140                 


Glu Asn Thr Ser Ile Ala Thr Val Asp Ala Ser Gly Lys Val Thr Leu 
145                 150                 155                 160 


Asn Gly Lys Gly Ser Val Val Ile Lys Ala Thr Ser Gly Asp Lys Gln 
                165                 170                 175     


Thr Val Ser Tyr Thr Ile Lys Ala Pro Ser Tyr Met Ile Lys Val Asp 
            180                 185                 190         


Lys Gln Ala Tyr Tyr Ala Asp Ala Met Ser Ile Cys Lys Asn Leu Leu 
        195                 200                 205             


Pro Ser Thr Gln Thr Val Leu Ser Asp Ile Tyr Asp Ser Trp Gly Ala 
    210                 215                 220                 


Ala Asn Lys Tyr Ser His Tyr Ser Ser Met Asn Ser Ile Thr Ala Trp 
225                 230                 235                 240 


Ile Lys Gln Thr Ser Ser Glu Gln Arg Ser Gly Val Ser Ser Thr Tyr 
                245                 250                 255     


Asn Leu Ile Thr Gln Asn Pro Leu Pro Gly Val Asn Val Asn Thr Pro 
            260                 265                 270         


Asn Val Tyr Ala Val Cys Val Glu 
        275                 280 


