                         SEQUENCE LISTING

<110>  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
 
<120>  COMPOSITIONS AND METHODS FOR DETECTING CELLS UNDERGOING 
       FERROPTOSIS USING AN ANTIBODY

<130>  1035795.000631

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a heavy chain

<400>  1

Glu Val Leu Leu Gln Gln Ser Gly Thr Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Leu Val Lys Leu Ser Cys Lys Ala Ser Gly Phe Asn Ile Gln Asp Leu 
            20                  25                  30          


Tyr Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asp Pro Glu Thr Asp Asn Thr Ile Tyr Asp Pro Lys Phe 
    50                  55                  60                  


Gln Gly Lys Ala Ser Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ser Thr Gly Leu Leu Gln Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr 
            100                 105                 110         


Ala Val Thr Val Ser Ser 
        115             


<210>  2
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a Kappa chain

<400>  2

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ala Met Ser Ile Gly 
1               5                   10                  15      


Gln Lys Val Thr Met Arg Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Tyr Asn Gln Lys Asn Cys Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Leu Tyr Phe Val Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Thr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR1

<400>  3

Asp Leu Tyr Ile His 
1               5   


<210>  4
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR2

<400>  4

Trp Ile Asp Pro Glu Thr Asp Asn Thr Ile Tyr Asp Pro Lys Phe Gln 
1               5                   10                  15      


Gly Lys Ala Ser 
            20  


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR3

<400>  5

Gly Leu Leu Gln Trp Tyr Phe Asp Val 
1               5                   


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR1

<400>  6

Lys Ser Ser Gln Ser Leu Leu Asn Ser Tyr Asn Gln Lys Asn Cys Leu 
1               5                   10                  15      


Ala 
    


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR2

<400>  7

Phe Val Ser Thr Arg Glu Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR3

<400>  8

Gln Gln His Tyr Ser Thr Pro Leu Thr 
1               5                   


<210>  9
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a heavy chain

<400>  9
gaggttctgc tgcagcagtc tgggactgag cttgtgaggc caggggcctt agtcaagttg       60

tcctgtaaag cttctggctt caacattcaa gacttatata tacactgggt gaaacagagg      120

cctgaacagg gcctggagtg gattggatgg attgatccag agactgataa tactatatat      180

gacccgaagt tccagggcaa ggccagtata acagcagaca catcctccaa cacagcctac      240

ctacaactca gcagcctgac atctgaggac actgccatgt attactgttc tacgggatta      300

ctacagtggt acttcgatgt ctggggcgct gggaccgcgg tcaccgtctc ctca            354


<210>  10
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a Kappa chain

<400>  10
gacattgtga tgacacagtc tccatcctcc ctggctatgt cgattggaca gaaggtcact       60

atgagatgca agtccagtca gagcctttta aatagttaca atcaaaaaaa ctgtttggcc      120

tggtaccagc agaaaccagg acagtctcct aaacttctgc tctactttgt atccactagg      180

gagtctgggg tccctgatcg cttcataggc agtggatctg ggacagattt cactcttacc      240

atcagcagtg tgcaggctga agacctggca gattacttct gtcagcaaca ttatagtact      300

ccgctcacgt tcggtgctgg gaccaagctg gagctgaaa                             339


<210>  11
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR1

<400>  11
gacttatata tacac                                                        15


<210>  12
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR2

<400>  12
tggattggat ggattgatcc agagactgat aatactatat atgacccgaa gttccagggc       60

aaggccagt                                                               69


<210>  13
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a HCDR3

<400>  13
ggattactac agtggtactt cgatgtc                                           27


<210>  14
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR1

<400>  14
aagtccagtc agagcctttt aaatagttac aatcaaaaaa actgtttggc c                51


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR2

<400>  15
tttgtatcca ctagggagtc t                                                 21


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3F3a LCDR3

<400>  16
cagcaacatt atagtactcc gctcacg                                           27


<210>  17
<211>  760
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Met Asp Gln Ala Arg Ser Ala Phe Ser Asn Leu Phe Gly Gly Glu 
1               5                   10                  15      


Pro Leu Ser Tyr Thr Arg Phe Ser Leu Ala Arg Gln Val Asp Gly Asp 
            20                  25                  30          


Asn Ser His Val Glu Met Lys Leu Ala Val Asp Glu Glu Glu Asn Ala 
        35                  40                  45              


Asp Asn Asn Thr Lys Ala Asn Val Thr Lys Pro Lys Arg Cys Ser Gly 
    50                  55                  60                  


Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile Val Phe Phe Leu Ile Gly 
65                  70                  75                  80  


Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys Gly Val Glu Pro Lys Thr 
                85                  90                  95      


Glu Cys Glu Arg Leu Ala Gly Thr Glu Ser Pro Val Arg Glu Glu Pro 
            100                 105                 110         


Gly Glu Asp Phe Pro Ala Ala Arg Arg Leu Tyr Trp Asp Asp Leu Lys 
        115                 120                 125             


Arg Lys Leu Ser Glu Lys Leu Asp Ser Thr Asp Phe Thr Gly Thr Ile 
    130                 135                 140                 


Lys Leu Leu Asn Glu Asn Ser Tyr Val Pro Arg Glu Ala Gly Ser Gln 
145                 150                 155                 160 


Lys Asp Glu Asn Leu Ala Leu Tyr Val Glu Asn Gln Phe Arg Glu Phe 
                165                 170                 175     


Lys Leu Ser Lys Val Trp Arg Asp Gln His Phe Val Lys Ile Gln Val 
            180                 185                 190         


Lys Asp Ser Ala Gln Asn Ser Val Ile Ile Val Asp Lys Asn Gly Arg 
        195                 200                 205             


Leu Val Tyr Leu Val Glu Asn Pro Gly Gly Tyr Val Ala Tyr Ser Lys 
    210                 215                 220                 


Ala Ala Thr Val Thr Gly Lys Leu Val His Ala Asn Phe Gly Thr Lys 
225                 230                 235                 240 


Lys Asp Phe Glu Asp Leu Tyr Thr Pro Val Asn Gly Ser Ile Val Ile 
                245                 250                 255     


Val Arg Ala Gly Lys Ile Thr Phe Ala Glu Lys Val Ala Asn Ala Glu 
            260                 265                 270         


Ser Leu Asn Ala Ile Gly Val Leu Ile Tyr Met Asp Gln Thr Lys Phe 
        275                 280                 285             


Pro Ile Val Asn Ala Glu Leu Ser Phe Phe Gly His Ala His Leu Gly 
    290                 295                 300                 


Thr Gly Asp Pro Tyr Thr Pro Gly Phe Pro Ser Phe Asn His Thr Gln 
305                 310                 315                 320 


Phe Pro Pro Ser Arg Ser Ser Gly Leu Pro Asn Ile Pro Val Gln Thr 
                325                 330                 335     


Ile Ser Arg Ala Ala Ala Glu Lys Leu Phe Gly Asn Met Glu Gly Asp 
            340                 345                 350         


Cys Pro Ser Asp Trp Lys Thr Asp Ser Thr Cys Arg Met Val Thr Ser 
        355                 360                 365             


Glu Ser Lys Asn Val Lys Leu Thr Val Ser Asn Val Leu Lys Glu Ile 
    370                 375                 380                 


Lys Ile Leu Asn Ile Phe Gly Val Ile Lys Gly Phe Val Glu Pro Asp 
385                 390                 395                 400 


His Tyr Val Val Val Gly Ala Gln Arg Asp Ala Trp Gly Pro Gly Ala 
                405                 410                 415     


Ala Lys Ser Gly Val Gly Thr Ala Leu Leu Leu Lys Leu Ala Gln Met 
            420                 425                 430         


Phe Ser Asp Met Val Leu Lys Asp Gly Phe Gln Pro Ser Arg Ser Ile 
        435                 440                 445             


Ile Phe Ala Ser Trp Ser Ala Gly Asp Phe Gly Ser Val Gly Ala Thr 
    450                 455                 460                 


Glu Trp Leu Glu Gly Tyr Leu Ser Ser Leu His Leu Lys Ala Phe Thr 
465                 470                 475                 480 


Tyr Ile Asn Leu Asp Lys Ala Val Leu Gly Thr Ser Asn Phe Lys Val 
                485                 490                 495     


Ser Ala Ser Pro Leu Leu Tyr Thr Leu Ile Glu Lys Thr Met Gln Asn 
            500                 505                 510         


Val Lys His Pro Val Thr Gly Gln Phe Leu Tyr Gln Asp Ser Asn Trp 
        515                 520                 525             


Ala Ser Lys Val Glu Lys Leu Thr Leu Asp Asn Ala Ala Phe Pro Phe 
    530                 535                 540                 


Leu Ala Tyr Ser Gly Ile Pro Ala Val Ser Phe Cys Phe Cys Glu Asp 
545                 550                 555                 560 


Thr Asp Tyr Pro Tyr Leu Gly Thr Thr Met Asp Thr Tyr Lys Glu Leu 
                565                 570                 575     


Ile Glu Arg Ile Pro Glu Leu Asn Lys Val Ala Arg Ala Ala Ala Glu 
            580                 585                 590         


Val Ala Gly Gln Phe Val Ile Lys Leu Thr His Asp Val Glu Leu Asn 
        595                 600                 605             


Leu Asp Tyr Glu Arg Tyr Asn Ser Gln Leu Leu Ser Phe Val Arg Asp 
    610                 615                 620                 


Leu Asn Gln Tyr Arg Ala Asp Ile Lys Glu Met Gly Leu Ser Leu Gln 
625                 630                 635                 640 


Trp Leu Tyr Ser Ala Arg Gly Asp Phe Phe Arg Ala Thr Ser Arg Leu 
                645                 650                 655     


Thr Thr Asp Phe Gly Asn Ala Glu Lys Thr Asp Arg Phe Val Met Lys 
            660                 665                 670         


Lys Leu Asn Asp Arg Val Met Arg Val Glu Tyr His Phe Leu Ser Pro 
        675                 680                 685             


Tyr Val Ser Pro Lys Glu Ser Pro Phe Arg His Val Phe Trp Gly Ser 
    690                 695                 700                 


Gly Ser His Thr Leu Pro Ala Leu Leu Glu Asn Leu Lys Leu Arg Lys 
705                 710                 715                 720 


Gln Asn Asn Gly Ala Phe Asn Glu Thr Leu Phe Arg Asn Gln Leu Ala 
                725                 730                 735     


Leu Ala Thr Trp Thr Ile Gln Gly Ala Ala Asn Ala Leu Ser Gly Asp 
            740                 745                 750         


Val Trp Asp Ile Asp Asn Glu Phe 
        755                 760 


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TfR1 primer FWD

<400>  18
accattgtca tatacccggt tca                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TfR1 primer RV

<400>  19
caatagccca agtagccaat cat                                               23


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH primer FWD

<400>  20
ctccaaaatc aagtggggcg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH primer RV

<400>  21
atgacgaaca tgggggcatc                                                   20


<210>  22
<211>  875
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target gene scFv for pcDNA3.1

<400>  22
ggatccgccg ccaccatgca cagctcagca ctgctctgtt gcctggtcct cctgactggg       60

gtgagggccg aagtcctcct ccaacaatcc ggaacagaac tcgttagacc tggtgcactg      120

gtcaagctct cctgtaaagc cagtggattc aacattcagg acctctacat tcactgggtc      180

aagcaacgcc cagagcaagg gctggagtgg atcggctgga ttgatcccga gacagataac      240

acaatctacg atcctaagtt ccagggtaag gcatccataa ctgccgacac aagcagtaat      300

actgcatacc tccagctgtc atcactcacc agcgaggata cagccatgta ctactgttcc      360

actggactgc tgcaatggta ctttgatgtt tggggagcag ggactgccgt taccgtgtcc      420

tctggaggcg gagggtccgg tggaggaggc tccggaggtg gtggcagcga catagtcatg      480

acacagagcc caagcagcct ggctatgagc atcgggcaga aggtgacaat gcggtgcaag      540

tcctctcaga gtctgctgaa ctcctacaat cagaagaatt gtctggcctg gtatcaacag      600

aagccaggcc aaagtcctaa gctcctcctg tatttcgttt caactcgcga gtctggtgtg      660

cctgacagat tcatcggcag tgggagcggg acagacttca ctctcaccat cagtagtgtc      720

caggctgagg acctggccga ctatttctgt caacaacatt actcaactcc tctgactttc      780

ggtgctggaa ccaaactgga gctgaagggc agcgagcaga aactgatttc cgaagaggac      840

ctgagccacc atcaccacca tcactaatag aattc                                 875


<210>  23
<211>  812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target gene scFv for pET28a

<400>  23
ccatggaagt gctgctgcaa caaagcggca ccgaactggt acgtccgggt gctctggtga       60

aactgtcttg taaagcatct ggtttcaaca tccaggacct gtacattcac tgggttaaac      120

agcgtccgga acaaggcctg gaatggatcg gttggatcga cccggaaact gacaacacta      180

tctacgaccc gaaattccaa ggtaaagcaa gcattaccgc agacacctct tccaacaccg      240

cgtacctgca actgtcctct ctgacctctg aagataccgc aatgtactac tgtagcactg      300

gtctgctgca atggtacttt gatgtttggg gtgccggtac tgcggtgact gtttcctctg      360

gtggcggtgg ttctggcggt ggtggttctg gtggtggtgg ctctgacatt gtgatgaccc      420

agtctccgag cagcctggcg atgtccatcg gtcagaaggt tactatgcgc tgcaagtcct      480

cccagtccct gctgaactcc tataaccaga agaattgtct ggcttggtat cagcagaaac      540

cgggtcaatc tccgaagctg ctgctgtact ttgtttctac tcgtgagtcc ggtgtaccag      600

atcgctttat cggttccggt tctggcaccg acttcaccct gaccatcagc tccgttcagg      660

cggaggatct ggccgactat ttctgccagc aacactatag cactccgctg acctttggtg      720

ctggcaccaa actggaactg aagggttctg agcagaaact gattagcgaa gaggatctgt      780

ctcatcacca ccaccatcat taatagctcg ag                                    812


<210>  24
<211>  6274
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pcDNA3.1(+)-scFv full sequence

<400>  24
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgagctcgg atccgccgcc accatgcaca gctcagcact      960

gctctgttgc ctggtcctcc tgactggggt gagggccgaa gtcctcctcc aacaatccgg     1020

aacagaactc gttagacctg gtgcactggt caagctctcc tgtaaagcca gtggattcaa     1080

cattcaggac ctctacattc actgggtcaa gcaacgccca gagcaagggc tggagtggat     1140

cggctggatt gatcccgaga cagataacac aatctacgat cctaagttcc agggtaaggc     1200

atccataact gccgacacaa gcagtaatac tgcatacctc cagctgtcat cactcaccag     1260

cgaggataca gccatgtact actgttccac tggactgctg caatggtact ttgatgtttg     1320

gggagcaggg actgccgtta ccgtgtcctc tggaggcgga gggtccggtg gaggaggctc     1380

cggaggtggt ggcagcgaca tagtcatgac acagagccca agcagcctgg ctatgagcat     1440

cgggcagaag gtgacaatgc ggtgcaagtc ctctcagagt ctgctgaact cctacaatca     1500

gaagaattgt ctggcctggt atcaacagaa gccaggccaa agtcctaagc tcctcctgta     1560

tttcgtttca actcgcgagt ctggtgtgcc tgacagattc atcggcagtg ggagcgggac     1620

agacttcact ctcaccatca gtagtgtcca ggctgaggac ctggccgact atttctgtca     1680

acaacattac tcaactcctc tgactttcgg tgctggaacc aaactggagc tgaagggcag     1740

cgagcagaaa ctgatttccg aagaggacct gagccaccat caccaccatc actaatagaa     1800

ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc     1860

tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     1920

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     1980

gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc     2040

aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct     2100

tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc     2160

gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc     2220

ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc     2280

cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc     2340

gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg     2400

gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact     2460

ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt     2520

tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt     2580

ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     2640

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     2700

gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc     2760

cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa     2820

ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt     2880

gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca     2940

ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat     3000

tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac     3060

agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc     3120

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc     3180

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag     3240

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc     3300

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg     3360

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc     3420

ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc     3480

cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga     3540

cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca     3600

tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg     3660

atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg     3720

ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg     3780

gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga     3840

ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg     3900

gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat     3960

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     4020

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg     4080

tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg     4140

aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc     4200

ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt     4260

ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg     4320

cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     4380

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     4440

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     4500

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     4560

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     4620

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     4680

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     4740

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     4800

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     4860

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     4920

agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg     4980

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     5040

aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     5100

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     5160

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     5220

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     5280

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     5340

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     5400

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     5460

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     5520

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     5580

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     5640

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     5700

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     5760

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     5820

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     5880

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     5940

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     6000

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     6060

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     6120

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     6180

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     6240

gcgcacattt ccccgaaaag tgccacctga cgtc                                 6274


<210>  25
<211>  6037
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET28a(+)-scFv full sequence

<400>  25
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagctattaa tgatggtggt      180

ggtgatgaga cagatcctct tcgctaatca gtttctgctc agaacccttc agttccagtt      240

tggtgccagc accaaaggtc agcggagtgc tatagtgttg ctggcagaaa tagtcggcca      300

gatcctccgc ctgaacggag ctgatggtca gggtgaagtc ggtgccagaa ccggaaccga      360

taaagcgatc tggtacaccg gactcacgag tagaaacaaa gtacagcagc agcttcggag      420

attgacccgg tttctgctga taccaagcca gacaattctt ctggttatag gagttcagca      480

gggactggga ggacttgcag cgcatagtaa ccttctgacc gatggacatc gccaggctgc      540

tcggagactg ggtcatcaca atgtcagagc caccaccacc agaaccacca ccgccagaac      600

caccgccacc agaggaaaca gtcaccgcag taccggcacc ccaaacatca aagtaccatt      660

gcagcagacc agtgctacag tagtacattg cggtatcttc agaggtcaga gaggacagtt      720

gcaggtacgc ggtgttggaa gaggtgtctg cggtaatgct tgctttacct tggaatttcg      780

ggtcgtagat agtgttgtca gtttccgggt cgatccaacc gatccattcc aggccttgtt      840

ccggacgctg tttaacccag tgaatgtaca ggtcctggat gttgaaacca gatgctttac      900

aagacagttt caccagagca cccggacgta ccagttcggt gccgctttgt tgcagcagca      960

cttccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta     1020

tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc     1080

tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct     1140

atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt     1200

gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct     1260

tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct     1320

tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc     1380

aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat     1440

gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt     1500

tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg     1560

gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag     1620

tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc     1680

gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa     1740

cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt     1800

gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc     1860

actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt     1920

ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag     1980

caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc     2040

tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg     2100

agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact     2160

gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc     2220

gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca     2280

tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc     2340

gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc     2400

gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc     2460

gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag     2520

tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat     2580

gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt     2640

cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct     2700

ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct     2760

tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa     2820

acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat     2880

cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa     2940

tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg     3000

agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc     3060

agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg     3120

aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc     3180

ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc     3240

atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat     3300

gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa     3360

catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga     3420

cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg     3480

cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga     3540

gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc     3600

agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt     3660

gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc     3720

ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt     3780

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     3840

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     3900

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     3960

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     4020

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     4080

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     4140

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     4200

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     4260

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     4320

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     4380

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     4440

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     4500

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     4560

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca     4620

ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg     4680

gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat     4740

aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag     4800

cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca     4860

gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat     4920

tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca     4980

ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg     5040

ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta     5100

tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt     5160

gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg     5220

ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt     5280

gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac     5340

caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg     5400

ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg     5460

ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa     5520

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa     5580

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     5640

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     5700

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     5760

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     5820

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     5880

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     5940

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     6000

gcttaatgcg ccgctacagg gcgcgtccca ttcgcca                              6037


