                         SEQUENCE LISTING

<110>  University of Cape Town
 
<120>  Plant Produced Bovine Papillomavirus Virus-Like Particles and 
       Psedovirions

<130>  PA173571/PCT

<150>  ZA 2020/00826
<151>  2020-02-10

<150>  GB 2013703.0
<151>  2020-09-01

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  495
<212>  PRT
<213>  Bovine Papillomavirus

<400>  1

Met Ala Leu Trp Gln Gln Gly Gln Lys Leu Tyr Leu Pro Pro Thr Pro 
1               5                   10                  15      


Val Ser Lys Val Leu Cys Ser Glu Thr Tyr Val Gln Arg Lys Ser Ile 
            20                  25                  30          


Phe Tyr His Ala Glu Thr Glu Arg Leu Leu Thr Ile Gly His Pro Tyr 
        35                  40                  45              


Tyr Pro Val Ser Ile Gly Ala Lys Thr Val Pro Lys Val Ser Ala Asn 
    50                  55                  60                  


Gln Tyr Arg Val Phe Lys Ile Gln Leu Pro Asp Pro Asn Gln Phe Ala 
65                  70                  75                  80  


Leu Pro Asp Arg Thr Val His Asn Pro Ser Lys Glu Arg Leu Val Trp 
                85                  90                  95      


Ala Val Ile Gly Val Gln Val Ser Arg Gly Gln Pro Leu Gly Gly Thr 
            100                 105                 110         


Val Thr Gly His Pro Thr Phe Asn Ala Leu Leu Asp Ala Glu Asn Val 
        115                 120                 125             


Asn Arg Lys Val Thr Thr Gln Thr Thr Asp Asp Arg Lys Gln Thr Gly 
    130                 135                 140                 


Leu Asp Ala Lys Gln Gln Gln Ile Leu Leu Leu Gly Cys Thr Pro Ala 
145                 150                 155                 160 


Glu Gly Glu Tyr Trp Thr Thr Ala Arg Pro Cys Val Thr Asp Arg Leu 
                165                 170                 175     


Glu Asn Gly Ala Cys Pro Pro Leu Glu Leu Lys Asn Lys His Ile Glu 
            180                 185                 190         


Asp Gly Asp Met Met Glu Ile Gly Phe Gly Ala Ala Asn Phe Lys Glu 
        195                 200                 205             


Ile Asn Ala Ser Lys Ser Asp Leu Pro Leu Asp Ile Gln Asn Glu Ile 
    210                 215                 220                 


Cys Leu Tyr Pro Asp Tyr Leu Lys Met Ala Glu Asp Ala Ala Gly Asn 
225                 230                 235                 240 


Ser Met Phe Phe Phe Ala Arg Lys Glu Gln Val Tyr Val Arg His Ile 
                245                 250                 255     


Trp Thr Arg Gly Gly Ser Glu Lys Glu Ala Pro Thr Thr Asp Phe Tyr 
            260                 265                 270         


Leu Lys Asn Asn Lys Gly Asp Ala Thr Leu Lys Ile Pro Ser Val His 
        275                 280                 285             


Phe Gly Ser Pro Ser Gly Ser Leu Val Ser Thr Asp Asn Gln Ile Phe 
    290                 295                 300                 


Asn Arg Pro Tyr Trp Leu Phe Arg Ala Gln Gly Met Asn Asn Gly Ile 
305                 310                 315                 320 


Ala Trp Asn Asn Leu Leu Phe Leu Thr Val Gly Asp Asn Thr Arg Gly 
                325                 330                 335     


Thr Asn Leu Thr Ile Ser Val Ala Ser Asp Gly Thr Pro Leu Thr Glu 
            340                 345                 350         


Tyr Asp Ser Ser Lys Phe Asn Val Tyr His Arg His Met Glu Glu Tyr 
        355                 360                 365             


Lys Leu Ala Phe Ile Leu Glu Leu Cys Ser Val Glu Ile Thr Ala Gln 
    370                 375                 380                 


Thr Val Ser His Leu Gln Gly Leu Met Pro Ser Val Leu Glu Asn Trp 
385                 390                 395                 400 


Glu Ile Gly Val Gln Pro Pro Thr Ser Ser Ile Leu Glu Asp Thr Tyr 
                405                 410                 415     


Arg Tyr Ile Glu Ser Pro Ala Thr Lys Cys Ala Ser Asn Val Ile Pro 
            420                 425                 430         


Ala Lys Glu Asp Pro Tyr Ala Gly Phe Lys Phe Trp Asn Ile Asp Leu 
        435                 440                 445             


Lys Glu Lys Leu Ser Leu Asp Leu Asp Gln Phe Pro Leu Gly Arg Arg 
    450                 455                 460                 


Phe Leu Ala Gln Gln Gly Ala Gly Cys Ser Thr Val Arg Lys Arg Arg 
465                 470                 475                 480 


Ile Ser Gln Lys Thr Ser Ser Lys Pro Ala Lys Lys Lys Lys Lys 
                485                 490                 495 


<210>  2
<211>  469
<212>  PRT
<213>  Bovine Papillomavirus

<400>  2

Met Ser Ala Arg Lys Arg Val Lys Arg Ala Ser Ala Tyr Asp Leu Tyr 
1               5                   10                  15      


Arg Thr Cys Lys Gln Ala Gly Thr Cys Pro Pro Asp Val Ile Pro Lys 
            20                  25                  30          


Val Glu Gly Asp Thr Ile Ala Asp Lys Ile Leu Lys Phe Gly Gly Leu 
        35                  40                  45              


Ala Ile Tyr Leu Gly Gly Leu Gly Ile Gly Thr Trp Ser Thr Gly Arg 
    50                  55                  60                  


Val Ala Ala Gly Gly Ser Pro Arg Tyr Thr Pro Leu Arg Thr Ala Gly 
65                  70                  75                  80  


Ser Thr Ser Ser Leu Ala Ser Ile Gly Ser Arg Ala Val Thr Ala Gly 
                85                  90                  95      


Thr Arg Pro Ser Ile Gly Ala Gly Ile Pro Leu Asp Thr Leu Glu Thr 
            100                 105                 110         


Leu Gly Ala Leu Arg Pro Gly Val Tyr Glu Asp Thr Val Leu Pro Glu 
        115                 120                 125             


Ala Pro Ala Ile Val Thr Pro Asp Ala Val Pro Ala Asp Ser Gly Leu 
    130                 135                 140                 


Asp Ala Leu Ser Ile Gly Thr Asp Ser Ser Thr Glu Thr Leu Ile Thr 
145                 150                 155                 160 


Leu Leu Glu Pro Glu Gly Pro Glu Asp Ile Ala Val Leu Glu Leu Gln 
                165                 170                 175     


Pro Leu Asp Arg Pro Thr Trp Gln Val Ser Asn Ala Val His Gln Ser 
            180                 185                 190         


Ser Ala Tyr His Ala Pro Leu Gln Leu Gln Ser Ser Ile Ala Glu Thr 
        195                 200                 205             


Ser Gly Leu Glu Asn Ile Phe Val Gly Gly Ser Gly Leu Gly Asp Thr 
    210                 215                 220                 


Gly Gly Glu Asn Ile Glu Leu Thr Tyr Phe Gly Ser Pro Arg Thr Ser 
225                 230                 235                 240 


Thr Pro Arg Ser Ile Ala Ser Lys Ser Arg Gly Ile Leu Asn Trp Phe 
                245                 250                 255     


Ser Lys Arg Tyr Tyr Thr Gln Val Pro Thr Glu Asp Pro Glu Val Phe 
            260                 265                 270         


Ser Ser Gln Thr Phe Ala Asn Pro Leu Tyr Glu Ala Glu Pro Ala Val 
        275                 280                 285             


Leu Lys Gly Pro Ser Gly Arg Val Gly Leu Ser Gln Val Tyr Lys Pro 
    290                 295                 300                 


Asp Thr Leu Thr Thr Arg Ser Gly Thr Glu Val Gly Pro Gln Leu His 
305                 310                 315                 320 


Val Arg Tyr Ser Leu Ser Thr Ile His Glu Asp Val Glu Ala Ile Pro 
                325                 330                 335     


Tyr Thr Val Asp Glu Asn Thr Gln Gly Leu Ala Phe Val Pro Leu His 
            340                 345                 350         


Glu Glu Gln Ala Gly Phe Glu Glu Ile Glu Leu Asp Asp Phe Ser Glu 
        355                 360                 365             


Thr His Arg Leu Leu Pro Gln Asn Thr Ser Ser Thr Pro Val Gly Ser 
    370                 375                 380                 


Gly Val Arg Arg Ser Leu Ile Pro Thr Gln Glu Phe Ser Ala Thr Arg 
385                 390                 395                 400 


Pro Thr Gly Val Val Thr Tyr Gly Ser Pro Asp Thr Tyr Ser Ala Ser 
                405                 410                 415     


Pro Val Thr Asp Pro Asp Ser Thr Ser Pro Ser Leu Val Ile Asp Asp 
            420                 425                 430         


Thr Thr Thr Thr Pro Ile Ile Ile Ile Asp Gly His Thr Val Asp Leu 
        435                 440                 445             


Tyr Ser Ser Asn Tyr Thr Leu His Pro Ser Leu Leu Arg Lys Arg Lys 
    450                 455                 460                 


Lys Arg Lys His Ala 
465                 


<210>  3
<211>  1508
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BPV1 L1 N. benthamiana-codon optimised

<400>  3
acgcgtaccg gtccatggcg ttgtggcagc agggtcaaaa actctatctc ccacctactc       60

cagtgtcaaa agtgttatgt tcagaaacct atgttcaaag gaaatcaatt ttctatcatg      120

ctgaaaccga gcgtcttttg actataggcc acccgtatta ccctgtttct attggggcta      180

agactgttcc taaagtgtca gcaaatcagt acagggtgtt caaaatacag ctgcctgatc      240

caaatcaatt tgctcttccg gaccgtacag ttcataaccc ctctaaggaa cgacttgtct      300

gggctgttat tggcgttcaa gtttctagag gtcaaccgtt gggtggaact gttacaggtc      360

accctacttt taatgcttta ctcgatgcag agaatgttaa ccggaaagtg actacacaga      420

ccacggatga caggaagcaa actggattgg atgctaaaca acagcaaatt ctgctacttg      480

gttgtacacc agccgaagga gagtattgga ctacagctag accttgtgtt accgatagac      540

ttgaaaatgg tgcttgtcct cctcttgaac tgaagaacaa acatatagaa gatggagata      600

tgatggagat tggtttcgga gctgcaaact tcaaggaaat taatgcttct aagtctgatc      660

ttcctcttga cattcagaat gagatctgct tatatccaga ttacctcaag atggctgagg      720

acgccgcggg taactcaatg tttttctttg caagaaagga gcaagtttat gttagacata      780

tctggactag aggtggtagt gaaaaggaag ctccaaccac ggatttctac ttgaagaata      840

acaaaggaga tgcaactctg aagatcccta gtgtccactt tggttctcca tcaggttctc      900

ttgtttctac agataaccag attttcaaca gaccttattg gctttttagg gcacagggta      960

tgaataacgg tatcgcctgg aataacttgt tatttctcac agttggagat aataccagag     1020

gaacgaatct tactattagc gtggcttccg atggtacacc tttaaccgaa tacgattctt     1080

ctaagtttaa tgtttaccat cgacacatgg aagagtacaa acttgctttt attttggaac     1140

tgtgttcagt cgagataacg gcacagactg tttctcatct tcaaggtctt atgccttccg     1200

ttcttgaaaa ttgggagatt ggagtgcagc ctcctacttc ttctatcctt gaagatacct     1260

atagatacat cgagtcgcct gccactaagt gtgcgagtaa tgtgatccct gccaaggaag     1320

atccatacgc gggtttcaaa ttctggaaca tcgatttgaa ggagaagctc tctctggatc     1380

ttgaccaatt cccactggga agaagatttc ttgctcagca aggcgcaggg tgctctacag     1440

ttaggaaaag gagaatcagt caaaaaacct catcaaagcc tgctaagaag aagaagaaat     1500

agctcgag                                                              1508


<210>  4
<211>  1430
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BPV1 L2 N. benthamiana-codon optimised

<400>  4
acgcgtaccg gttcatgagt gctaggaaaa gggttaaaag agcttctgct tatgatttgt       60

ataggacttg taaacaggct ggaacttgcc caccagatgt gatccctaag gttgaaggag      120

atacaatcgc agacaagata ctgaaatttg gtggtcttgc catctatctt ggtggtcttg      180

gcattggtac ttggagtact ggtagagttg ctgctggtgg ttctcctaga tacactcctc      240

ttcgaacagc tggtagtacc tcttcattgg caagcattgg ttctagagct gttactgcag      300

gaacaaggcc atcaattggt gctggaatcc ctctggatac ccttgaaact cttggggcac      360

ttagacctgg tgtctatgaa gatacagtat tgcctgaggc cccagcgatt gttactccgg      420

atgccgtgcc cgcggatagc ggtttagatg ctctttccat cggtaccgat tcttctaccg      480

aaactttgat aactcttttg gaaccggagg gacccgaaga tattgctgtg cttgaactac      540

aacctcttga caggccaaca tggcaagttt ctaatgcagt acatcagtcc tcggcttatc      600

acgcacctct tcaattgcag tcttcaatag ctgaaacttc aggtcttgag aatatttttg      660

ttggtggtag tggtcttgga gatactggtg gagaaaacat tgagttaaca tacttcggtt      720

ctcctagaac ttctactcct agatctatcg cttctaagtc tagaggtatc cttaactggt      780

tctctaagag atactacacg caagtcccga ctgaagatcc cgaggttttc tcttctcaga      840

cattcgcaaa ccctctgtat gaagccgagc cagcggttct aaagggcccg agtggtagag      900

ttggtttaag ccaagtatac aaaccagata cactcactac tagatcagga accgaagttg      960

gccctcagtt acatgtgagg tatagtctca gcactattca cgaagatgtt gaggctatcc     1020

cttacacagt ggacgagaat acccaaggac ttgcctttgt gccattgcat gaagagcagg     1080

ctggttttga agagattgaa cttgatgact tctctgagac acatagactt ctccctcaaa     1140

acacttcttc tacaccagtt ggttcaggag tgagaaggag tcttatccct actcaggagt     1200

tctctgctac caggcctact ggtgttgtga cctatggttc tcctgatact tactccgcat     1260

ctcctgttac tgatccagac tctacatcac ctagtcttgt gattgatgac accacgacta     1320

cacctattat tattattgat ggacatactg ttgatcttta ctcatctaac tacacactcc     1380

atccatcatt actcaggaag aggaagaaga ggaaacacgc ttgactcgag                1430


