                         SEQUENCE LISTING

<110>  Oxford BioMedica (UK) Limited
 
<120>  PRODUCTION OF LENTIVIRAL VECTORS

<130>  P118969PCT

<140>  PCT/GB2021/050247
<141>  2021-02-04

<150>  GB 2001996.4
<151>  2020-02-13

<150>  GB 2017820.8
<151>  2020-11-11

<160>  242   

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  splice donor region

<400>  1
ggggcggcga ctggtgagta cgccaaaaat                                        30


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region, MSD-2KO

<400>  2
ggggcggcga ctgcagacaa cgccaaaaat                                        30


<210>  3
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  splice donor site consensus sequence

<400>  3
tggtragt                                                                 8


<210>  4
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  major splice donor consensus sequence

<400>  4
ctggt                                                                    5


<210>  5
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  splice donor region

<400>  5
cagaca                                                                   6


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  6
ggcgactgca gacaacgcc                                                    19


<210>  7
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  7
gtggagact                                                                9


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  8
ggcgagtgga gactacgcc                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  splice donor region

<400>  9
ggcgactggt gagtacgcc                                                    19


<210>  10
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cryptic splice donor site consensus sequence

<400>  10
tgagt                                                                    5


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region, MSD-2KOv2

<400>  11
ggggcggcga gtggagacta cgccaaaaat                                        30


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region, MSD-2KOm5

<400>  12
ggggaaggca acagataaat atgccttaaa at                                     32


<210>  13
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  major splice donor and cryptic splice donor region core sequence

<400>  13
gtgagta                                                                  7


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor region

<400>  14
aaggcaacag ataaatatgc ctt                                               23


<210>  15
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  256U1 snRNA sequence

<400>  15
taaggaccag cttctttggg agagaacaga cgcaggggcg ggagggaaaa agggagaggc       60

agacgtcact tccccttggc ggctctggca gcagattggt cggttgagtg gcagaaaggc      120

agacggggac tgggcaaggc actgtcggtg acatcacgga cagggcgact tctatgtaga      180

tgaggcagcg cagaggctgc tgcttcgcca cttgctgctt caccacgaag gagttcccgt      240

gccctgggag cgggttcagg accgctgatc ggaagtgaga atcccagctg tgtgtcaggg      300

ctggaaaggg ctcgggagtg cgcggggcaa gtgaccgtgt gtgtaaagag tgaggcgtat      360

gaggctgtgt cggggcagag gcccaagatc tcatttgccg tgcgcgcttg caggggagat      420

accatgatca cgaaggtggt tttcccaggg cgaggcttat ccattgcact ccggatgtgc      480

tgacccctgc gatttcccca aatgtgggaa actcgactgc ataatttgtg gtagtggggg      540

actgcgttcg cgctttcccc tggtttcaaa agtagactgt acgctaaggg tcatatcttt      600

ttttgttttg gtttgtgtct tggttggcgt cttaaatgtt aa                         642


<210>  16
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  16
gaccagatct gagcc                                                        15


<210>  17
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  17
atggctcaga tctggtc                                                      17


<210>  18
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  18
tgggagctct ctggc                                                        15


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  19
atgccagaga gctccca                                                      17


<210>  20
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  20
taaagcttgc cttga                                                        15


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  21
attcaaggca agcttta                                                      17


<210>  22
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  22
tagagatccc tcaga                                                        15


<210>  23
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  23
attctgaggg atctcta                                                      17


<210>  24
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  24
gcagtggcg                                                                9


<210>  25
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  25
atcgccactg c                                                            11


<210>  26
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  26
agtggcgccc gaaca                                                        15


<210>  27
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  27
attgttcggg cgccact                                                      17


<210>  28
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  28
gggacttgaa agcga                                                        15


<210>  29
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  29
attcgctttc aagtccc                                                      17


<210>  30
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  30
aagggaaacc agagg                                                        15


<210>  31
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  31
atcctctggt ttccctt                                                      17


<210>  32
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  32
ggactcggct tgctg                                                        15


<210>  33
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  33
atcagcaagc cgagtcc                                                      17


<210>  34
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  34
aagcgcgcac ggcaa                                                        15


<210>  35
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  35
atttgccgtg cgcgctt                                                      17


<210>  36
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  36
gaggcgaggg gcggc                                                        15


<210>  37
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  37
atgccgcccc tcgcctc                                                      17


<210>  38
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  38
gactggtgag tacgc                                                        15


<210>  39
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  39
atgcgtactc accagtc                                                      17


<210>  40
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  40
aattttgact a                                                            11


<210>  41
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  41
atgtcaaaat t                                                            11


<210>  42
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  42
aattttgact agcgg                                                        15


<210>  43
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  43
atccgctagt caaaatt                                                      17


<210>  44
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  44
gcggaggcta gaagg                                                        15


<210>  45
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  45
atccttctag cctccgc                                                      17


<210>  46
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  46
agagagatgg gtgcg                                                        15


<210>  47
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  47
atcgcaccca tctctct                                                      17


<210>  48
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  48
agagcgtcgg tatta                                                        15


<210>  49
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  49
attaatactg acgctct                                                      17


<210>  50
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  50
agcgggggag aatta                                                        15


<210>  51
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  51
attaattctc ccccgct                                                      17


<210>  52
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  52
gatcgcgatg ggaaa                                                        15


<210>  53
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  53
attttcccat cgcgatc                                                      17


<210>  54
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  54
aaattcggtt aaggc                                                        15


<210>  55
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  55
atgccttaac cgaattt                                                      17


<210>  56
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  56
gatcttcaga cctgg                                                        15


<210>  57
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  57
atccaggtct gaagatc                                                      17


<210>  58
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  58
ttacacaagc ttaat                                                        15


<210>  59
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  59
atattaagct tgtgtaa                                                      17


<210>  60
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  60
tagtagacat aatag                                                        15


<210>  61
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  61
atctattatg tctacta                                                      17


<210>  62
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Control U1 snRNA target sequence

<400>  62
ctacaggaa                                                                9


<210>  63
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  63
atttcctgta g                                                            11


<210>  64
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Control U1 snRNA target sequence

<400>  64
tcatctgtg                                                                9


<210>  65
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  65
atcacagatg a                                                            11


<210>  66
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified U1 snRNA sequence (main U1 snRNA sequence [clover leaf] 
       (nt 410-562) of the U1_256 sequence)

<400>  66
gcaggggaga taccatgatc acgaaggtgg ttttcccagg gcgaggctta tccattgcac       60

tccggatgtg ctgacccctg cgatttcccc aaatgtggga aactcgactg cataatttgt      120

ggtagtgggg gactgcgttc gcgctttccc ctg                                   153


<210>  67
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 target sequence

<400>  67
agctctctcg acgca                                                        15


<210>  68
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA target-annealing sequence

<400>  68
attgcgtcga gagagct                                                      17


<210>  69
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  69
gagaatg                                                                  7


<210>  70
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  70
gagcatg                                                                  7


<210>  71
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  71
gaggatg                                                                  7


<210>  72
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  72
tagaatg                                                                  7


<210>  73
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  73
tagcatg                                                                  7


<210>  74
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  74
taggatg                                                                  7


<210>  75
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  75
gagaaatg                                                                 8


<210>  76
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  76
gagacatg                                                                 8


<210>  77
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  77
gagagatg                                                                 8


<210>  78
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  78
gagcaatg                                                                 8


<210>  79
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  79
gagccatg                                                                 8


<210>  80
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  80
gagcgatg                                                                 8


<210>  81
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  81
gaggaatg                                                                 8


<210>  82
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  82
gaggcatg                                                                 8


<210>  83
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  83
gagggatg                                                                 8


<210>  84
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  84
tagaaatg                                                                 8


<210>  85
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  85
tagacatg                                                                 8


<210>  86
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  86
tagagatg                                                                 8


<210>  87
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  87
tagcaatg                                                                 8


<210>  88
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  88
tagccatg                                                                 8


<210>  89
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  89
tagcgatg                                                                 8


<210>  90
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  90
taggaatg                                                                 8


<210>  91
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  91
taggcatg                                                                 8


<210>  92
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  92
tagggatg                                                                 8


<210>  93
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L33 improved leader derived from a splicing event

<400>  93
ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gaaaa                       45


<210>  94
<211>  75
<212>  PRT
<213>  Bacillus subtilis

<400>  94

Met Asn Gln Lys His Ser Ser Asp Phe Val Val Ile Lys Ala Val Glu 
1               5                   10                  15      


Asp Gly Val Asn Val Ile Gly Leu Thr Arg Gly Thr Asp Thr Lys Phe 
            20                  25                  30          


His His Ser Glu Lys Leu Asp Lys Gly Glu Val Ile Ile Ala Gln Phe 
        35                  40                  45              


Thr Glu His Thr Ser Ala Ile Lys Val Arg Gly Glu Ala Leu Ile Gln 
    50                  55                  60                  


Thr Ala Tyr Gly Glu Met Lys Ser Glu Lys Lys 
65                  70                  75  


<210>  95
<211>  86
<212>  PRT
<213>  Aminomonas paucivorans

<400>  95

Met Lys Glu Gly Glu Glu Ala Lys Thr Ser Val Leu Ser Asp Tyr Val 
1               5                   10                  15      


Val Val Lys Ala Leu Glu Asn Gly Val Thr Val Ile Gly Leu Thr Arg 
            20                  25                  30          


Gly Gln Glu Thr Lys Phe Ala His Thr Glu Lys Leu Asp Asp Gly Glu 
        35                  40                  45              


Val Trp Ile Ala Gln Phe Thr Glu His Thr Ser Ala Ile Lys Val Arg 
    50                  55                  60                  


Gly Ala Ser Glu Ile His Thr Lys His Gly Met Leu Phe Ser Gly Arg 
65                  70                  75                  80  


Gly Arg Asn Glu Lys Gly 
                85      


<210>  96
<211>  81
<212>  PRT
<213>  Desulfotomaculum hydrothermale

<400>  96

Met Asn Pro Met Thr Asp Arg Ser Asp Ile Thr Gly Asp Tyr Val Val 
1               5                   10                  15      


Val Lys Ala Leu Glu Asn Gly Val Thr Ile Ile Gly Leu Thr Arg Gly 
            20                  25                  30          


Gly Val Thr Lys Phe His His Thr Glu Lys Leu Asp Lys Gly Glu Ile 
        35                  40                  45              


Met Ile Ala Gln Phe Thr Glu His Thr Ser Ala Ile Lys Ile Arg Gly 
    50                  55                  60                  


Arg Ala Glu Leu Leu Thr Lys His Gly Lys Ile Arg Thr Glu Val Asp 
65                  70                  75                  80  


Ser 
    


<210>  97
<211>  74
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  97

Met Tyr Thr Asn Ser Asp Phe Val Val Ile Lys Ala Leu Glu Asp Gly 
1               5                   10                  15      


Val Asn Val Ile Gly Leu Thr Arg Gly Ala Asp Thr Arg Phe His His 
            20                  25                  30          


Ser Glu Lys Leu Asp Lys Gly Glu Val Leu Ile Ala Gln Phe Thr Glu 
        35                  40                  45              


His Thr Ser Ala Ile Lys Val Arg Gly Lys Ala Tyr Ile Gln Thr Arg 
    50                  55                  60                  


His Gly Val Ile Glu Ser Glu Gly Lys Lys 
65                  70                  


<210>  98
<211>  74
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  98

Met Tyr Thr Asn Ser Asp Phe Val Val Ile Lys Ala Leu Glu Asp Gly 
1               5                   10                  15      


Val Asn Val Ile Gly Leu Thr Arg Gly Ala Asp Thr Arg Phe His His 
            20                  25                  30          


Ser Glu Lys Leu Asp Lys Gly Glu Val Leu Ile Ala Gln Phe Thr Glu 
        35                  40                  45              


His Thr Ser Ala Ile Lys Val Arg Gly Lys Ala Tyr Ile Gln Thr Arg 
    50                  55                  60                  


His Gly Val Ile Glu Asn Glu Gly Lys Lys 
65                  70                  


<210>  99
<211>  76
<212>  PRT
<213>  Bacillus halodurans

<400>  99

Met Asn Val Gly Asp Asn Ser Asn Phe Phe Val Ile Lys Ala Lys Glu 
1               5                   10                  15      


Asn Gly Val Asn Val Phe Gly Met Thr Arg Gly Thr Asp Thr Arg Phe 
            20                  25                  30          


His His Ser Glu Lys Leu Asp Lys Gly Glu Val Met Ile Ala Gln Phe 
        35                  40                  45              


Thr Glu His Thr Ser Ala Val Lys Ile Arg Gly Lys Ala Ile Ile Gln 
    50                  55                  60                  


Thr Ser Tyr Gly Thr Leu Asp Thr Glu Lys Asp Glu 
65                  70                  75      


<210>  100
<211>  80
<212>  PRT
<213>  Carboxydothermus hydrogenoformans

<400>  100

Met Val Cys Asp Asn Phe Ala Phe Ser Ser Ala Ile Asn Ala Glu Tyr 
1               5                   10                  15      


Ile Val Val Lys Ala Leu Glu Asn Gly Val Thr Ile Met Gly Leu Thr 
            20                  25                  30          


Arg Gly Lys Asp Thr Lys Phe His His Thr Glu Lys Leu Asp Lys Gly 
        35                  40                  45              


Glu Val Met Val Ala Gln Phe Thr Glu His Thr Ser Ala Ile Lys Ile 
    50                  55                  60                  


Arg Gly Lys Ala Glu Ile Tyr Thr Lys His Gly Val Ile Lys Asn Glu 
65                  70                  75                  80  


<210>  101
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L33 improved leader

<400>  101
ctttttcgca acgggtttgc cgccagaaca cag                                    33


<210>  102
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L12 improved leader

<400>  102
ctttttcgca ac                                                           12


<210>  103
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  extended Kozak consensus sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<400>  103
gnnrvvatg                                                                9


<210>  104
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  core Kozak consensus sequence

<400>  104
rvvatg                                                                   6


<210>  105
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EMCV M loops

<400>  105
cgtggttttc ctttgaaaaa cacgatgata cc                                     32


<210>  106
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal (overlapping) tbs-Kozak

<400>  106
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagacgagcc gagatg           56


<210>  107
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal (overlapping) tbs-MCS-Kozak

<400>  107
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagacgagaa gagctctaga       60

ccatg                                                                   65


<210>  108
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  108
tagatg                                                                   6


<210>  109
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  109
gagatatg                                                                 8


<210>  110
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  110
gagctatg                                                                 8


<210>  111
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  111
tagatatg                                                                 8


<210>  112
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  112
tagctatg                                                                 8


<210>  113
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak consensus sequence


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<400>  113
kagnng                                                                   6


<210>  114
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak consensus sequence

<400>  114
kagatg                                                                   6


<210>  115
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak consensus sequence


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<400>  115
kagnntg                                                                  7


<210>  116
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak consensus sequence


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<400>  116
kagnatg                                                                  7


<210>  117
<211>  1082
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chicken beta-Actin / Rabbit beta-globin chimeric 5'UTR-intron 
       with tbs-kzkV0.G variant

<400>  117
cggcgggcgg gaacgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctccc      120

tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga      180

aagccttaaa gggctccggg agggcctttg tgcggggggg agcggctcgg ggggtgcgtg      240

cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc      300

tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcgggccggg      360

ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt      420

gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctggca      480

cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg      540

tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc      600

ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc      660

ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg      720

cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc      780

cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg      840

ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag      900

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc      960

ggcggcttta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg     1020

ggcaaagagt ttagcggagt ggagaagagc ggagccgagc ctagcagaga cgagccgaga     1080

tg                                                                    1082


<210>  118
<211>  1040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1a 5'UTR-intron with tbskzkV0.G variant

<400>  118
ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg       60

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag      120

tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gtggccttgc      180

gcttaaggag ccccttcgcc tcgtgcttga gttgtggcct ggcctgggcg ctggggccgc      240

cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc      300

atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat      360

gcgggccaag atcagcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc      420

cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc      480

ggacgggggt agtctcaagc tgcccggcct gctctggtgc ctggcctcgc gccgccgtgt      540

atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga      600

tggccgcttc ccggccctgc tgcagggagc acaaaatgga ggacgcggcg ctcgggagag      660

cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca      720

tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc cagcttttgg      780

agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag      840

tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc      900

ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt      960

cttccatttc aggtgtcgtg aaaagagttt agcggagtgg agaagagcgg agccgagcct     1020

agcagagacg agccgagatg                                                 1040


<210>  119
<211>  161
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spliced sequence corresponding to SEQ ID NO: 117

<400>  119
cggcgggcgg gaacgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca cagctcctgg gcaaagagtt tagcggagtg      120

gagaagagcg gagccgagcc tagcagagac gagccgagat g                          161


<210>  120
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spliced sequence corresponding to SEQ ID NO: 118

<400>  120
ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gaaaagagtt tagcggagtg       60

gagaagagcg gagccgagcc tagcagagac gagccgagat g                          101


<210>  121
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chicken beta-Actin / Rabbit beta-globin chimeric 5'UTR-intron

<400>  121
cggcgggcgg gaacgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctccc      120

tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga      180

aagccttaaa gggctccggg agggcctttg tgcggggggg agcggctcgg ggggtgcgtg      240

cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc      300

tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcgggccggg      360

ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt      420

gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctggca      480

cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg      540

tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc      600

ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc      660

ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg      720

cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc      780

cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg      840

ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag      900

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc      960

ggcggcttta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg     1020

ggcaaa                                                                1026


<210>  122
<211>  984
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1a 5'UTR-intron

<400>  122
ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg       60

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag      120

tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gtggccttgc      180

gcttaaggag ccccttcgcc tcgtgcttga gttgtggcct ggcctgggcg ctggggccgc      240

cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc      300

atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat      360

gcgggccaag atcagcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc      420

cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc      480

ggacgggggt agtctcaagc tgcccggcct gctctggtgc ctggcctcgc gccgccgtgt      540

atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga      600

tggccgcttc ccggccctgc tgcagggagc acaaaatgga ggacgcggcg ctcgggagag      660

cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca      720

tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc cagcttttgg      780

agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag      840

tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc      900

ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt      960

cttccatttc aggtgtcgtg aaaa                                             984


<210>  123
<211>  1030
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chicken beta-Actin / Rabbit beta-globin chimeric 5'UTR-intron-tbs
       consensus


<220>
<221>  misc_feature
<222>  (1027)..(1030)
<223>  kagn(2-3) may be repeated 10-11 times

<220>
<221>  misc_feature
<222>  (1030)..(1030)
<223>  n may be repeated 2-3 times

<400>  123
cggcgggcgg gaacgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctccc      120

tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga      180

aagccttaaa gggctccggg agggcctttg tgcggggggg agcggctcgg ggggtgcgtg      240

cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc      300

tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcgggccggg      360

ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt      420

gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctggca      480

cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg      540

tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc      600

ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc      660

ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg      720

cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc      780

cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg      840

ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag      900

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc      960

ggcggcttta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg     1020

ggcaaaksgn                                                            1030


<210>  124
<211>  988
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1a 5'UTR-intron-tbs consensus


<220>
<221>  misc_feature
<222>  (985)..(988)
<223>  kagn(2-3) may be repeated 10-11 times

<220>
<221>  misc_feature
<222>  (988)..(988)
<223>  n may be repeated 2-3 times

<400>  124
ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg       60

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag      120

tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gtggccttgc      180

gcttaaggag ccccttcgcc tcgtgcttga gttgtggcct ggcctgggcg ctggggccgc      240

cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc      300

atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat      360

gcgggccaag atcagcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc      420

cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc      480

ggacgggggt agtctcaagc tgcccggcct gctctggtgc ctggcctcgc gccgccgtgt      540

atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga      600

tggccgcttc ccggccctgc tgcagggagc acaaaatgga ggacgcggcg ctcgggagag      660

cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca      720

tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc cagcttttgg      780

agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag      840

tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc      900

ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt      960

cttccatttc aggtgtcgtg aaaakagn                                         988


<210>  125
<211>  55
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]11

<400>  125
gaguuuagcg gaguggagaa gagcggagcc gagccuagca gagacgagug gagcu            55


<210>  126
<211>  55
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]11

<400>  126
gaguuuagcg gaguggagaa gagcggagcc gagccuagca gagacgagaa gagcu            55


<210>  127
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]6

<400>  127
uaguuuaguu uaguuuaguu uaguuuaguu                                        30


<210>  128
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]6

<400>  128
uaguuuaguu gaguuuaguu gaguuuaguu                                        30


<210>  129
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]6

<400>  129
gaguuugagu ugaguugagu uugaguugag uu                                     32


<210>  130
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]6

<400>  130
uaguuugagu uuaguugagu uuuaguugag uu                                     32


<210>  131
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]11

<400>  131
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagacgagtg gagct            55


<210>  132
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]10

<400>  132
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagacgagaa                  50


<210>  133
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]9

<400>  133
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagac                       45


<210>  134
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]8

<400>  134
gagtttagcg gagtggagaa gagcggagcc gagcctagca                             40


<210>  135
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]7

<400>  135
gagtttagcg gagtggagaa gagcggagcc gagcc                                  35


<210>  136
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]6

<400>  136
gagtttagcg gagtggagaa gagcggagcc                                        30


<210>  137
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNNN]3[KAGNN]8

<400>  137
gagtttagcg gagtggagaa agagacggag ccgagaccta gcagagacga gaagagct         58


<210>  138
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNNN]1[KAGNN]10

<400>  138
gagtttagcg gagtggagaa gagacggagc cgagcctagc agagacgaga agagct           56


<210>  139
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNN]11

<400>  139
gagtttagcg gagtggagaa gagcggagcc gagcctagca gagacgagaa gagct            55


<210>  140
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNNN]1[KAGNN]7

<400>  140
gagtttagcg gagtggagaa agagcggagc cgagcctagc a                           41


<210>  141
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAP binding site variant - [KAGNNN]1[KAGNN]6

<400>  141
gagtttagcg gagtggagaa agagcggagc cgagcc                                 36


<210>  142
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  142
gagatg                                                                   6


<210>  143
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  143
kagvatg                                                                  7


<210>  144
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  144
kagvvatg                                                                 8


<210>  145
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  145
kagrvvatg                                                                9


<210>  146
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<400>  146
kagnrvvatg                                                              10


<210>  147
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal 3' tbs-Kozak junction variant

<400>  147
kagccgagat g                                                            11


<210>  148
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal 3' tbs-Kozak junction variant


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, or t

<400>  148
kagnggagcc atg                                                          13


<210>  149
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal 3' tbs-Kozak junction variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<400>  149
kagnngagac catg                                                         14


<210>  150
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-Kozak junction variant

<400>  150
kaggcgagca tg                                                           12


<210>  151
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  151
atagcagaga cggct                                                        15


<210>  152
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  152
atagcagaga                                                              10


<210>  153
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  153
atagc                                                                    5


<210>  154
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  154
atatcagaga cggctagcgt atacca                                            26


<210>  155
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  155
atatcagaga cggct                                                        15


<210>  156
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  156
agagacggct                                                              10


<210>  157
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer variant

<400>  157
tacca                                                                    5


<210>  158
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  158
gagctctaga vvatg                                                        15


<210>  159
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  159
gagctcgtcg acvatg                                                       16


<210>  160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  160
gagctcgaat tcgaavvatg                                                   20


<210>  161
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  161
gagctctaga cgtcgacvat g                                                 21


<210>  162
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  162
gagctctaga attcgaavva tg                                                22


<210>  163
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  163
gagctctaga tatcgatrvv atg                                               23


<210>  164
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  164
kagactagta cttaagcttr vvatg                                             25


<210>  165
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  165
gagctctaga ccatg                                                        15


<210>  166
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  166
gagctcgtcg accatg                                                       16


<210>  167
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  167
gagctcgaat tcgaaccatg                                                   20


<210>  168
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  168
gagctctaga cgtcgaccat g                                                 21


<210>  169
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  169
gagctctaga attcgaacca tg                                                22


<210>  170
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  170
gagctctaga tatcgatacc atg                                               23


<210>  171
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' tbs-MCS-Kozak variant

<400>  171
kagactagta cttaagctta ccatg                                             25


<210>  172
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 1 containing L33 Improved 
       leader, optimal (overlapping) tbs ([KAGNN]8)-Kozak junction

<400>  172
ctttttcgca acgggtttgc cgccagaaca caggagttta gcggagtgga gaagagcgga       60

gccgagccga gatg                                                         74


<210>  173
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 2 containing L33 Improved 
       leader, optimal (overlapping) tbs ([KAGNN]11)-Kozak junction

<400>  173
ctttttcgca acgggtttgc cgccagaaca caggagttta gcggagtgga gaagagcgga       60

gccgagccta gcagagacga gccgagatg                                         89


<210>  174
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 3 containing L12 Improved 
       leader, optimal (overlapping) tbs ([KAGNN]11)-Kozak junction

<400>  174
ctttttcgca acgagtttag cggagtggag aagagcggag ccgagcctag cagagacgag       60

ccgagatg                                                                68


<210>  175
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 4 for intron-containing 
       5'UTRs, resulting in a spliced leader comprising L33, optimal 
       (overlapping) tbs ([KAGNN]11)-Kozak junction

<400>  175
ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gaaaagagtt tagcggagtg       60

gagaagagcg gagccgagcc tagcagagac gagccgagat g                          101


<210>  176
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 5 containing improved spacer, 
       optimal (overlapping) tbs ([KAGNN]8)-Kozak junction

<400>  176
atagcagaga cggctgagtt tagcggagtg gagaagagcg gagccgagcc gagatg           56


<210>  177
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 6 containing L33 Improved 
       leader tbs ([KAGNN]11)-MCS-Kozak

<400>  177
ctttttcgca acgggtttgc cgccagaaca caggagttta gcggagtgga gaagagcgga       60

gccgagccta gcagagacga gaagagctct agaccatg                               98


<210>  178
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  illustrative nucleic acid sequence 7 containing improved spacer, 
       tbs ([KAGNN]11)-MCS-Kozak

<400>  178
atagcagaga cggctgagtt tagcggagtg gagaagagcg gagccgagcc tagcagagac       60

gagaagagct ctagaccatg                                                   80


<210>  179
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tbs-Kozak region

<400>  179
acagccacca tg                                                           12


<210>  180
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HpaI variant tbs-Kozak region

<400>  180
gagttaacgc caccatg                                                      17


<210>  181
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  consensus Kozak sequence (DNA)

<400>  181
gccgccrcca tgg                                                          13


<210>  182
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus Kozak sequence (RNA)

<400>  182
gccgccrcca ugg                                                          13


<210>  183
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  extended Kozak consensus sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or u

<400>  183
gnnrvvaugg                                                              10


<210>  184
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  original 3' tbs-Kozak junction sequence


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<400>  184
kagnnacagc caccatg                                                      17


<210>  185
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (54)..(55)
<223>  n is a, c, g, or t

<400>  185
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnacagc       60

caccatg                                                                 67


<210>  186
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  186
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagatg           56


<210>  187
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  187
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagatg           56


<210>  188
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  188
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagcatg          57


<210>  189
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  189
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagaatg          57


<210>  190
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  190
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gaggatg          57


<210>  191
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  191
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagaatg          57


<210>  192
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  192
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagcatg          57


<210>  193
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  193
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn taggatg          57


<210>  194
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  194
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagccatg         58


<210>  195
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  195
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagaaatg         58


<210>  196
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  196
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagagatg         58


<210>  197
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  197
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagacatg         58


<210>  198
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  198
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagatatg         58


<210>  199
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  199
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagcaatg         58


<210>  200
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  200
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagcgatg         58


<210>  201
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  201
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagctatg         58


<210>  202
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  202
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gaggaatg         58


<210>  203
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  203
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gagggatg         58


<210>  204
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  204
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn gaggcatg         58


<210>  205
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  205
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagaaatg         58


<210>  206
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  206
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagagatg         58


<210>  207
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  207
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagacatg         58


<210>  208
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  208
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagatatg         58


<210>  209
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  209
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagcaatg         58


<210>  210
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  210
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagcgatg         58


<210>  211
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  211
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagccatg         58


<210>  212
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  212
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagctatg         58


<210>  213
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  213
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn taggaatg         58


<210>  214
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  214
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn tagggatg         58


<210>  215
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  overlapping tbs 3'KAGNN-Kozak variant


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<400>  215
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn taggcatg         58


<210>  216
<211>  164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  U1 snRNA molecule

<400>  216
auacuuaccu ggcaggggag auaccaugau cacgaaggug guuuucccag ggcgaggcuu       60

auccauugca cuccggaugu gcugaccccu gcgauuuccc caaauguggg aaacucuacu      120

gcauaauuug ugguaguggg ggacugcguu cgcgcuuucc ccug                       164


<210>  217
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated splice donor (MSD) region, deltaSL2

<400>  217
ggggcgcaaa aat                                                          13


<210>  218
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tbs consensus sequence


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (54)..(55)
<223>  n is a, c, g, or t

<400>  218
kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnnkagnn kagnn            55


<210>  219
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild type SL2-MSD-SL4(STD) sequence including SL2 loop region

<400>  219
ggcggcgact ggtgagtacg ccaaaaat                                          28


<210>  220
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild type SL2-MSD-SL4(STD) sequence including SL4 loop region

<400>  220
gagatgggtg cgagagcgtc ag                                                22


<210>  221
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-1KO sequence including SL2 loop region

<400>  221
ggcggcgact gcagagtacg ccaaaaat                                          28


<210>  222
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-1KO sequence including SL4 loop region

<400>  222
gagatgggtg cgagagcgtc ag                                                22


<210>  223
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KO sequence including SL2 loop region

<400>  223
ggcggcgact gcagacaacg ccaaaaat                                          28


<210>  224
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KO sequence including SL4 loop region

<400>  224
gagatgggtg cgagagcgtc ag                                                22


<210>  225
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KO_1 sequence including SL2 loop region

<400>  225
ggcggcgact gcagacaacg ccaaaaat                                          28


<210>  226
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KO_1 sequence including SL4 loop region

<400>  226
gagatggctg cgagagcgtc ag                                                22


<210>  227
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KO_2 sequence including SL2 loop region

<400>  227
ggcggcgact gcagacaacg ccaaaaat                                          28


<210>  228
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KO_2 sequence including SL4 loop region

<400>  228
gagatgggcg cgagagcgtc ag                                                22


<210>  229
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KO_1 sequence including SL2 loop region

<400>  229
ggcggcgact gcagacaacg ccaaaaat                                          28


<210>  230
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KO_1 sequence including SL4 loop region

<400>  230
gagatggctg cgagagcatc ag                                                22


<210>  231
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KO_2 sequence including SL2 loop region

<400>  231
ggcggcgact gcagacaacg ccaaaaat                                          28


<210>  232
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KO_2 sequence including SL4 loop region

<400>  232
gagatgggcg cgagagcatc ag                                                22


<210>  233
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KOm5 sequence including SL2 loop region

<400>  233
ggaaggcaac agataaatat gccttaaaat                                        30


<210>  234
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-2KOm5 sequence including SL4 loop region

<400>  234
gagatgggtg cgagagcgtc ag                                                22


<210>  235
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KOm5_2 sequence including SL2 loop region

<400>  235
ggaaggcaac agataaatat gccttaaaat                                        30


<210>  236
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-3KOm5_2 sequence including SL4 loop region

<400>  236
gagatgggcg cgagagcgtc ag                                                22


<210>  237
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KOm5_2 sequence including SL2 loop region

<400>  237
ggaaggcaac agataaatat gccttaaaat                                        30


<210>  238
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSD-4KOm5_2 sequence including SL4 loop region

<400>  238
gagatgggcg cgagagcatc ag                                                22


<210>  239
<211>  11
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  endogenous U1 snRNA

<400>  239
auacuuaccu g                                                            11


<210>  240
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  wild type major splice donor site sequence, wt SL2-MSD(STD)

<400>  240
ggcgacuggu gaguacgcc                                                    19


<210>  241
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  variant major splice donor site, MSD-2KO

<400>  241
ggcgacugca gacaacgcc                                                    19


<210>  242
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  variant major splice donor site, MSD-2KOm5

<400>  242
ggcaacagau aaauaugcc                                                    19


