                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
 
<120>  Burden-addicted production strains

<130>  P2638EP00

<150>  EP20157159.3
<151>  2020-02-13

<160>  164   

<170>  PatentIn version 3.5

<210>  1
<211>  2179
<212>  DNA
<213>  E. coli


<220>
<221>  gene
<222>  (1)..(2179)
<223>  E. coli essential gene folP-glmM

<220>
<221>  CDS
<222>  (1)..(849)
<223>  folP

<400>  1
atg aaa ctc ttt gcc cag ggt act tca ctg gac ctt agc cat cct cac         48
Met Lys Leu Phe Ala Gln Gly Thr Ser Leu Asp Leu Ser His Pro His           
1               5                   10                  15                

gta atg ggg atc ctc aac gtc acg cct gat tcc ttt tcg gat ggt ggc         96
Val Met Gly Ile Leu Asn Val Thr Pro Asp Ser Phe Ser Asp Gly Gly           
            20                  25                  30                    

acg cat aac tcg ctg ata gat gcg gtg aaa cat gcg aat ctg atg atc        144
Thr His Asn Ser Leu Ile Asp Ala Val Lys His Ala Asn Leu Met Ile           
        35                  40                  45                        

aat gct ggc gcg acg atc att gac gtt ggt ggc gag tcc acg cgc cca        192
Asn Ala Gly Ala Thr Ile Ile Asp Val Gly Gly Glu Ser Thr Arg Pro           
    50                  55                  60                            

ggg gcg gcg gaa gtt agc gtt gaa gaa gag ttg caa cgt gtt att cct        240
Gly Ala Ala Glu Val Ser Val Glu Glu Glu Leu Gln Arg Val Ile Pro           
65                  70                  75                  80            

gtg gtt gag gca att gct caa cgc ttc gaa gtc tgg atc tcg gtc gat        288
Val Val Glu Ala Ile Ala Gln Arg Phe Glu Val Trp Ile Ser Val Asp           
                85                  90                  95                

aca tcc aaa cca gaa gtc atc cgt gag tca gcg aaa gtt ggc gct cac        336
Thr Ser Lys Pro Glu Val Ile Arg Glu Ser Ala Lys Val Gly Ala His           
            100                 105                 110                   

att att aat gat atc cgc tcc ctt tcc gaa cct ggc gct ctg gag gcg        384
Ile Ile Asn Asp Ile Arg Ser Leu Ser Glu Pro Gly Ala Leu Glu Ala           
        115                 120                 125                       

gct gca gaa acc ggt tta ccg gtt tgt ctg atg cat atg cag gga aat        432
Ala Ala Glu Thr Gly Leu Pro Val Cys Leu Met His Met Gln Gly Asn           
    130                 135                 140                           

cca aaa acc atg cag gaa gct ccg aag tat gac gat gtc ttt gca gaa        480
Pro Lys Thr Met Gln Glu Ala Pro Lys Tyr Asp Asp Val Phe Ala Glu           
145                 150                 155                 160           

gtg aat cgc tac ttt att gag caa ata gca cgt tgc gag cag gcg ggt        528
Val Asn Arg Tyr Phe Ile Glu Gln Ile Ala Arg Cys Glu Gln Ala Gly           
                165                 170                 175               

atc gca aaa gag aaa ttg ttg ctc gac ccc gga ttc ggt ttc ggt aaa        576
Ile Ala Lys Glu Lys Leu Leu Leu Asp Pro Gly Phe Gly Phe Gly Lys           
            180                 185                 190                   

aat ctc tcc cat aac tat tca tta ctg gcg cgc ctg gct gaa ttt cac        624
Asn Leu Ser His Asn Tyr Ser Leu Leu Ala Arg Leu Ala Glu Phe His           
        195                 200                 205                       

cat ttc aac ctg ccg ctg ttg gtg ggt atg tca cga aaa tcg atg att        672
His Phe Asn Leu Pro Leu Leu Val Gly Met Ser Arg Lys Ser Met Ile           
    210                 215                 220                           

ggg cag ctg ctg aac gtg ggg ccg tcc gag cgc ctg agc ggt agt ctg        720
Gly Gln Leu Leu Asn Val Gly Pro Ser Glu Arg Leu Ser Gly Ser Leu           
225                 230                 235                 240           

gcc tgt gcg gtc att gcc gca atg caa ggc gcg cac atc att cgt gtt        768
Ala Cys Ala Val Ile Ala Ala Met Gln Gly Ala His Ile Ile Arg Val           
                245                 250                 255               

cat gac gtc aaa gaa acc gta gaa gcg atg cgg gtg gtg gaa gcc act        816
His Asp Val Lys Glu Thr Val Glu Ala Met Arg Val Val Glu Ala Thr           
            260                 265                 270                   

ctg tct gca aag gaa aac aaa cgc tat gag taa tcgtaaatat ttcggtaccg      869
Leu Ser Ala Lys Glu Asn Lys Arg Tyr Glu                                   
        275                 280                                           

atgggattcg tggtcgtgta ggggatgcgc cgatcacacc tgattttgtg cttaagctgg      929

gttgggccgc gggtaaagtg ctggcgcgcc acggctcccg taagatcatt attggtaaag      989

acacgcgtat ttctggctat atgctggagt cagcactgga agcgggtctg gcggcagcgg     1049

gcctttccgc actcttcact ggcccgatgc caacaccggc cgtggcttat ctgacgcgta     1109

ccttccgcgc agaggccgga attgtgatat ctgcatcgca taacccgttc tacgataatg     1169

gcattaaatt cttctctatc gacggcacca aactgccgga tgcggtagaa gaggccatcg     1229

aagcggaaat ggaaaaggag atcagctgcg ttgattcggc agaactgggt aaagccagcc     1289

gtatcgttga tgccgcgggt cgctatatcg agttttgcaa agccacgttc ccgaacgaac     1349

ttagcctcag tgaactgaag attgtggtgg attgtgcaaa cggtgcgact tatcacatcg     1409

cgccgaacgt gctgcgcgaa ctgggggcga acgttatcgc tatcggttgt gagccaaacg     1469

gtgtaaacat caatgccgaa gtgggggcta ccgacgttcg cgcgctccag gctcgtgtgc     1529

tggctgaaaa agcggatctc ggtattgcct tcgacggcga tggcgatcgc gtgattatgg     1589

ttgaccatga aggcaataaa gtcgatggcg atcagatcat gtatatcatc gcgcgtgaag     1649

gtcttcgtca gggccagctg cgtggtggcg ctgtgggtac attgatgagc aacatggggc     1709

ttgaactggc gctgaaacag ttaggaattc catttgcgcg cgcgaaagtg ggtgaccgct     1769

acgtactgga aaaaatgcag gagaaaggct ggcgtatcgg tgcagagaat tccggtcatg     1829

tgatcctgct ggataaaact actaccggtg acggcatcgt tgctggcttg caggtgctgg     1889

cggctatggc acgtaaccat atgagcctgc acgacctttg cagcggcatg aaaatgttcc     1949

cgcagattct ggttaacgta cgttacaccg caggtagcgg cgatccactt gagcatgagt     2009

cagttaaagc cgtgaccgca gaggttgaag ctgcgctggg caaccgtgga cgcgtgttgc     2069

tgcgtaaatc cggcaccgaa ccgttaattc gcgtgatggt ggaaggcgaa gacgaagcgc     2129

aggtgactga atttgcacac cgcatcgccg atgcagtaaa agccgtttaa                2179


<210>  2
<211>  282
<212>  PRT
<213>  E. coli

<400>  2

Met Lys Leu Phe Ala Gln Gly Thr Ser Leu Asp Leu Ser His Pro His 
1               5                   10                  15      


Val Met Gly Ile Leu Asn Val Thr Pro Asp Ser Phe Ser Asp Gly Gly 
            20                  25                  30          


Thr His Asn Ser Leu Ile Asp Ala Val Lys His Ala Asn Leu Met Ile 
        35                  40                  45              


Asn Ala Gly Ala Thr Ile Ile Asp Val Gly Gly Glu Ser Thr Arg Pro 
    50                  55                  60                  


Gly Ala Ala Glu Val Ser Val Glu Glu Glu Leu Gln Arg Val Ile Pro 
65                  70                  75                  80  


Val Val Glu Ala Ile Ala Gln Arg Phe Glu Val Trp Ile Ser Val Asp 
                85                  90                  95      


Thr Ser Lys Pro Glu Val Ile Arg Glu Ser Ala Lys Val Gly Ala His 
            100                 105                 110         


Ile Ile Asn Asp Ile Arg Ser Leu Ser Glu Pro Gly Ala Leu Glu Ala 
        115                 120                 125             


Ala Ala Glu Thr Gly Leu Pro Val Cys Leu Met His Met Gln Gly Asn 
    130                 135                 140                 


Pro Lys Thr Met Gln Glu Ala Pro Lys Tyr Asp Asp Val Phe Ala Glu 
145                 150                 155                 160 


Val Asn Arg Tyr Phe Ile Glu Gln Ile Ala Arg Cys Glu Gln Ala Gly 
                165                 170                 175     


Ile Ala Lys Glu Lys Leu Leu Leu Asp Pro Gly Phe Gly Phe Gly Lys 
            180                 185                 190         


Asn Leu Ser His Asn Tyr Ser Leu Leu Ala Arg Leu Ala Glu Phe His 
        195                 200                 205             


His Phe Asn Leu Pro Leu Leu Val Gly Met Ser Arg Lys Ser Met Ile 
    210                 215                 220                 


Gly Gln Leu Leu Asn Val Gly Pro Ser Glu Arg Leu Ser Gly Ser Leu 
225                 230                 235                 240 


Ala Cys Ala Val Ile Ala Ala Met Gln Gly Ala His Ile Ile Arg Val 
                245                 250                 255     


His Asp Val Lys Glu Thr Val Glu Ala Met Arg Val Val Glu Ala Thr 
            260                 265                 270         


Leu Ser Ala Lys Glu Asn Lys Arg Tyr Glu 
        275                 280         


<210>  3
<211>  1338
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1338)
<223>  E. coli essential gene glmM

<400>  3
atg agt aat cgt aaa tat ttc ggt acc gat ggg att cgt ggt cgt gta         48
Met Ser Asn Arg Lys Tyr Phe Gly Thr Asp Gly Ile Arg Gly Arg Val           
1               5                   10                  15                

ggg gat gcg ccg atc aca cct gat ttt gtg ctt aag ctg ggt tgg gcc         96
Gly Asp Ala Pro Ile Thr Pro Asp Phe Val Leu Lys Leu Gly Trp Ala           
            20                  25                  30                    

gcg ggt aaa gtg ctg gcg cgc cac ggc tcc cgt aag att att att ggt        144
Ala Gly Lys Val Leu Ala Arg His Gly Ser Arg Lys Ile Ile Ile Gly           
        35                  40                  45                        

aaa gac acg cgt att tct ggc tat atg ctg gag tca gca ctg gaa gcg        192
Lys Asp Thr Arg Ile Ser Gly Tyr Met Leu Glu Ser Ala Leu Glu Ala           
    50                  55                  60                            

ggt ctg gcg gca gcg ggc ctt tcc gca ctc ttc act ggc ccg atg cca        240
Gly Leu Ala Ala Ala Gly Leu Ser Ala Leu Phe Thr Gly Pro Met Pro           
65                  70                  75                  80            

aca ccg gcc gtg gct tat ctg acg cgt acc ttc cgc gca gag gcc gga        288
Thr Pro Ala Val Ala Tyr Leu Thr Arg Thr Phe Arg Ala Glu Ala Gly           
                85                  90                  95                

att gtg ata tct gca tcg cat aac ccg ttc tac gat aat ggc att aaa        336
Ile Val Ile Ser Ala Ser His Asn Pro Phe Tyr Asp Asn Gly Ile Lys           
            100                 105                 110                   

ttc ttc tct atc gac ggc acc aaa ctg ccg gat gcg gta gaa gag gcc        384
Phe Phe Ser Ile Asp Gly Thr Lys Leu Pro Asp Ala Val Glu Glu Ala           
        115                 120                 125                       

atc gaa gcg gaa atg gaa aag gag atc agc tgc gtt gat tcg gca gaa        432
Ile Glu Ala Glu Met Glu Lys Glu Ile Ser Cys Val Asp Ser Ala Glu           
    130                 135                 140                           

ctg ggt aaa gcc agc cgt atc gtt gat gcc gcg ggt cgc tat atc gag        480
Leu Gly Lys Ala Ser Arg Ile Val Asp Ala Ala Gly Arg Tyr Ile Glu           
145                 150                 155                 160           

ttt tgc aaa gcc acg ttc ccg aac gaa ctt agc ctc agt gaa ctg aag        528
Phe Cys Lys Ala Thr Phe Pro Asn Glu Leu Ser Leu Ser Glu Leu Lys           
                165                 170                 175               

att gtg gtg gat tgt gca aac ggt gcg act tat cac atc gcg ccg aac        576
Ile Val Val Asp Cys Ala Asn Gly Ala Thr Tyr His Ile Ala Pro Asn           
            180                 185                 190                   

gtg ctg cgc gaa ctg ggg gcg aac gtt atc gct atc ggt tgt gag cca        624
Val Leu Arg Glu Leu Gly Ala Asn Val Ile Ala Ile Gly Cys Glu Pro           
        195                 200                 205                       

aac ggt gta aac atc aat gcc gaa gtg ggg gct acc gac gtt cgc gcg        672
Asn Gly Val Asn Ile Asn Ala Glu Val Gly Ala Thr Asp Val Arg Ala           
    210                 215                 220                           

ctc cag gct cgt gtg ctg gct gaa aaa gcg gat ctc ggt att gcc ttc        720
Leu Gln Ala Arg Val Leu Ala Glu Lys Ala Asp Leu Gly Ile Ala Phe           
225                 230                 235                 240           

gac ggc gat ggc gat cgc gtg att atg gtt gac cat gaa ggc aat aaa        768
Asp Gly Asp Gly Asp Arg Val Ile Met Val Asp His Glu Gly Asn Lys           
                245                 250                 255               

gtc gat ggc gat cag atc atg tat atc atc gcg cgt gaa ggt ctt cgt        816
Val Asp Gly Asp Gln Ile Met Tyr Ile Ile Ala Arg Glu Gly Leu Arg           
            260                 265                 270                   

cag ggc cag ctg cgt ggt ggc gct gtg ggt aca ttg atg agc aac atg        864
Gln Gly Gln Leu Arg Gly Gly Ala Val Gly Thr Leu Met Ser Asn Met           
        275                 280                 285                       

ggg ctt gaa ctg gcg ctg aaa cag tta gga att cca ttt gcg cgc gcg        912
Gly Leu Glu Leu Ala Leu Lys Gln Leu Gly Ile Pro Phe Ala Arg Ala           
    290                 295                 300                           

aaa gtg ggt gac cgc tac gta ctg gaa aaa atg cag gag aaa ggc tgg        960
Lys Val Gly Asp Arg Tyr Val Leu Glu Lys Met Gln Glu Lys Gly Trp           
305                 310                 315                 320           

cgt atc ggt gca gag aat tcc ggt cat gtg atc ctg ctg gat aaa act       1008
Arg Ile Gly Ala Glu Asn Ser Gly His Val Ile Leu Leu Asp Lys Thr           
                325                 330                 335               

act acc ggt gac ggc atc gtt gct ggc ttg cag gtg ctg gcg gcg atg       1056
Thr Thr Gly Asp Gly Ile Val Ala Gly Leu Gln Val Leu Ala Ala Met           
            340                 345                 350                   

gca cgt aac cat atg agc ctg cac gac ctt tgc agc ggc atg aaa atg       1104
Ala Arg Asn His Met Ser Leu His Asp Leu Cys Ser Gly Met Lys Met           
        355                 360                 365                       

ttc ccg cag att ctg gtt aac gta cgt tac acc gca ggt agc ggc gat       1152
Phe Pro Gln Ile Leu Val Asn Val Arg Tyr Thr Ala Gly Ser Gly Asp           
    370                 375                 380                           

cca ctt gag cat gag tca gtt aaa gcc gtg acc gca gag gtt gaa gct       1200
Pro Leu Glu His Glu Ser Val Lys Ala Val Thr Ala Glu Val Glu Ala           
385                 390                 395                 400           

gcg ctg ggc aac cgt gga cgc gtg ttg ctg cgt aaa tcc ggc acc gaa       1248
Ala Leu Gly Asn Arg Gly Arg Val Leu Leu Arg Lys Ser Gly Thr Glu           
                405                 410                 415               

ccg tta att cgc gtg atg gtg gaa ggc gaa gac gaa gcg cag gtg act       1296
Pro Leu Ile Arg Val Met Val Glu Gly Glu Asp Glu Ala Gln Val Thr           
            420                 425                 430                   

gaa ttt gca cac cgc atc gcc gat gca gta aaa gcc gtt taa               1338
Glu Phe Ala His Arg Ile Ala Asp Ala Val Lys Ala Val                       
        435                 440                 445                       


<210>  4
<211>  445
<212>  PRT
<213>  E. coli

<400>  4

Met Ser Asn Arg Lys Tyr Phe Gly Thr Asp Gly Ile Arg Gly Arg Val 
1               5                   10                  15      


Gly Asp Ala Pro Ile Thr Pro Asp Phe Val Leu Lys Leu Gly Trp Ala 
            20                  25                  30          


Ala Gly Lys Val Leu Ala Arg His Gly Ser Arg Lys Ile Ile Ile Gly 
        35                  40                  45              


Lys Asp Thr Arg Ile Ser Gly Tyr Met Leu Glu Ser Ala Leu Glu Ala 
    50                  55                  60                  


Gly Leu Ala Ala Ala Gly Leu Ser Ala Leu Phe Thr Gly Pro Met Pro 
65                  70                  75                  80  


Thr Pro Ala Val Ala Tyr Leu Thr Arg Thr Phe Arg Ala Glu Ala Gly 
                85                  90                  95      


Ile Val Ile Ser Ala Ser His Asn Pro Phe Tyr Asp Asn Gly Ile Lys 
            100                 105                 110         


Phe Phe Ser Ile Asp Gly Thr Lys Leu Pro Asp Ala Val Glu Glu Ala 
        115                 120                 125             


Ile Glu Ala Glu Met Glu Lys Glu Ile Ser Cys Val Asp Ser Ala Glu 
    130                 135                 140                 


Leu Gly Lys Ala Ser Arg Ile Val Asp Ala Ala Gly Arg Tyr Ile Glu 
145                 150                 155                 160 


Phe Cys Lys Ala Thr Phe Pro Asn Glu Leu Ser Leu Ser Glu Leu Lys 
                165                 170                 175     


Ile Val Val Asp Cys Ala Asn Gly Ala Thr Tyr His Ile Ala Pro Asn 
            180                 185                 190         


Val Leu Arg Glu Leu Gly Ala Asn Val Ile Ala Ile Gly Cys Glu Pro 
        195                 200                 205             


Asn Gly Val Asn Ile Asn Ala Glu Val Gly Ala Thr Asp Val Arg Ala 
    210                 215                 220                 


Leu Gln Ala Arg Val Leu Ala Glu Lys Ala Asp Leu Gly Ile Ala Phe 
225                 230                 235                 240 


Asp Gly Asp Gly Asp Arg Val Ile Met Val Asp His Glu Gly Asn Lys 
                245                 250                 255     


Val Asp Gly Asp Gln Ile Met Tyr Ile Ile Ala Arg Glu Gly Leu Arg 
            260                 265                 270         


Gln Gly Gln Leu Arg Gly Gly Ala Val Gly Thr Leu Met Ser Asn Met 
        275                 280                 285             


Gly Leu Glu Leu Ala Leu Lys Gln Leu Gly Ile Pro Phe Ala Arg Ala 
    290                 295                 300                 


Lys Val Gly Asp Arg Tyr Val Leu Glu Lys Met Gln Glu Lys Gly Trp 
305                 310                 315                 320 


Arg Ile Gly Ala Glu Asn Ser Gly His Val Ile Leu Leu Asp Lys Thr 
                325                 330                 335     


Thr Thr Gly Asp Gly Ile Val Ala Gly Leu Gln Val Leu Ala Ala Met 
            340                 345                 350         


Ala Arg Asn His Met Ser Leu His Asp Leu Cys Ser Gly Met Lys Met 
        355                 360                 365             


Phe Pro Gln Ile Leu Val Asn Val Arg Tyr Thr Ala Gly Ser Gly Asp 
    370                 375                 380                 


Pro Leu Glu His Glu Ser Val Lys Ala Val Thr Ala Glu Val Glu Ala 
385                 390                 395                 400 


Ala Leu Gly Asn Arg Gly Arg Val Leu Leu Arg Lys Ser Gly Thr Glu 
                405                 410                 415     


Pro Leu Ile Arg Val Met Val Glu Gly Glu Asp Glu Ala Gln Val Thr 
            420                 425                 430         


Glu Phe Ala His Arg Ile Ala Asp Ala Val Lys Ala Val 
        435                 440                 445 


<210>  5
<211>  858
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(858)
<223>  E. coli essential gene murI

<400>  5
atg gct acc aaa ctg cag gac ggg aat aca cct tgt ctg gca gct aca         48
Met Ala Thr Lys Leu Gln Asp Gly Asn Thr Pro Cys Leu Ala Ala Thr           
1               5                   10                  15                

cct tct gaa cca cgt ccc acc gtg ctg gtg ttt gac tcc ggc gtc ggt         96
Pro Ser Glu Pro Arg Pro Thr Val Leu Val Phe Asp Ser Gly Val Gly           
            20                  25                  30                    

ggg ttg tcg gtc tat gac gag atc cgg cat ctc tta ccg gat ctc cat        144
Gly Leu Ser Val Tyr Asp Glu Ile Arg His Leu Leu Pro Asp Leu His           
        35                  40                  45                        

tac att tat gct ttc gat aac gtc gct ttc ccg tat ggc gaa aaa agc        192
Tyr Ile Tyr Ala Phe Asp Asn Val Ala Phe Pro Tyr Gly Glu Lys Ser           
    50                  55                  60                            

gaa gcg ttt att gtt gag cga gtg gtg gca att gtc acc gcg gtg caa        240
Glu Ala Phe Ile Val Glu Arg Val Val Ala Ile Val Thr Ala Val Gln           
65                  70                  75                  80            

gaa cgt tat ccc ctt gcg ctg gct gtg gtc gct tgc aac act gcc agt        288
Glu Arg Tyr Pro Leu Ala Leu Ala Val Val Ala Cys Asn Thr Ala Ser           
                85                  90                  95                

acc gtt tca ctt cct gca tta cgc gaa aag ttc gac ttc ccg gtt gtt        336
Thr Val Ser Leu Pro Ala Leu Arg Glu Lys Phe Asp Phe Pro Val Val           
            100                 105                 110                   

ggt gtc gtg ccg gcg att aaa cct gct gca cgt ctg acg gca aat ggc        384
Gly Val Val Pro Ala Ile Lys Pro Ala Ala Arg Leu Thr Ala Asn Gly           
        115                 120                 125                       

att gtc gga tta ctg gca acc cgc gga aca gtt aaa cgt tct tat act        432
Ile Val Gly Leu Leu Ala Thr Arg Gly Thr Val Lys Arg Ser Tyr Thr           
    130                 135                 140                           

cat gag ctg atc gcg cgt ttc gct aat gaa tgc cag ata gaa atg ctg        480
His Glu Leu Ile Ala Arg Phe Ala Asn Glu Cys Gln Ile Glu Met Leu           
145                 150                 155                 160           

ggc tcg gca gag atg gtt gag ttg gct gaa gcg aag cta cat ggc gaa        528
Gly Ser Ala Glu Met Val Glu Leu Ala Glu Ala Lys Leu His Gly Glu           
                165                 170                 175               

gat gtt tct ctg gat gca cta aaa cgt atc cta cgc ccg tgg tta aga        576
Asp Val Ser Leu Asp Ala Leu Lys Arg Ile Leu Arg Pro Trp Leu Arg           
            180                 185                 190                   

atg aaa gag ccg cca gat acc gtt gta ttg ggt tgc acc cat ttc cct        624
Met Lys Glu Pro Pro Asp Thr Val Val Leu Gly Cys Thr His Phe Pro           
        195                 200                 205                       

cta cta caa gaa gaa ctg tta caa gtg ctg cca gag gga acc cgg ctg        672
Leu Leu Gln Glu Glu Leu Leu Gln Val Leu Pro Glu Gly Thr Arg Leu           
    210                 215                 220                           

gtg gat tct ggc gca gcg att gct cgc cga acg gcc tgg ttg tta gaa        720
Val Asp Ser Gly Ala Ala Ile Ala Arg Arg Thr Ala Trp Leu Leu Glu           
225                 230                 235                 240           

cat gaa gcc ccg gat gca aaa tct gcc gat gcg aat att gcc ttt tgt        768
His Glu Ala Pro Asp Ala Lys Ser Ala Asp Ala Asn Ile Ala Phe Cys           
                245                 250                 255               

atg gca atg acg cca gga gct gaa caa tta ttg ccc gtt tta cag cgt        816
Met Ala Met Thr Pro Gly Ala Glu Gln Leu Leu Pro Val Leu Gln Arg           
            260                 265                 270                   

tac ggc ttc gaa acg ctc gaa aaa ctg gca gtt tta ggc tga                858
Tyr Gly Phe Glu Thr Leu Glu Lys Leu Ala Val Leu Gly                       
        275                 280                 285                       


<210>  6
<211>  285
<212>  PRT
<213>  E. coli

<400>  6

Met Ala Thr Lys Leu Gln Asp Gly Asn Thr Pro Cys Leu Ala Ala Thr 
1               5                   10                  15      


Pro Ser Glu Pro Arg Pro Thr Val Leu Val Phe Asp Ser Gly Val Gly 
            20                  25                  30          


Gly Leu Ser Val Tyr Asp Glu Ile Arg His Leu Leu Pro Asp Leu His 
        35                  40                  45              


Tyr Ile Tyr Ala Phe Asp Asn Val Ala Phe Pro Tyr Gly Glu Lys Ser 
    50                  55                  60                  


Glu Ala Phe Ile Val Glu Arg Val Val Ala Ile Val Thr Ala Val Gln 
65                  70                  75                  80  


Glu Arg Tyr Pro Leu Ala Leu Ala Val Val Ala Cys Asn Thr Ala Ser 
                85                  90                  95      


Thr Val Ser Leu Pro Ala Leu Arg Glu Lys Phe Asp Phe Pro Val Val 
            100                 105                 110         


Gly Val Val Pro Ala Ile Lys Pro Ala Ala Arg Leu Thr Ala Asn Gly 
        115                 120                 125             


Ile Val Gly Leu Leu Ala Thr Arg Gly Thr Val Lys Arg Ser Tyr Thr 
    130                 135                 140                 


His Glu Leu Ile Ala Arg Phe Ala Asn Glu Cys Gln Ile Glu Met Leu 
145                 150                 155                 160 


Gly Ser Ala Glu Met Val Glu Leu Ala Glu Ala Lys Leu His Gly Glu 
                165                 170                 175     


Asp Val Ser Leu Asp Ala Leu Lys Arg Ile Leu Arg Pro Trp Leu Arg 
            180                 185                 190         


Met Lys Glu Pro Pro Asp Thr Val Val Leu Gly Cys Thr His Phe Pro 
        195                 200                 205             


Leu Leu Gln Glu Glu Leu Leu Gln Val Leu Pro Glu Gly Thr Arg Leu 
    210                 215                 220                 


Val Asp Ser Gly Ala Ala Ile Ala Arg Arg Thr Ala Trp Leu Leu Glu 
225                 230                 235                 240 


His Glu Ala Pro Asp Ala Lys Ser Ala Asp Ala Asn Ile Ala Phe Cys 
                245                 250                 255     


Met Ala Met Thr Pro Gly Ala Glu Gln Leu Leu Pro Val Leu Gln Arg 
            260                 265                 270         


Tyr Gly Phe Glu Thr Leu Glu Lys Leu Ala Val Leu Gly 
        275                 280                 285 


<210>  7
<211>  1104
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1104)
<223>  E. coli essential gene asd

<400>  7
atg aaa aat gtt ggt ttt atc ggc tgg cgc ggt atg gtc ggc tcc gtt         48
Met Lys Asn Val Gly Phe Ile Gly Trp Arg Gly Met Val Gly Ser Val           
1               5                   10                  15                

ctc atg caa cgc atg gtt gaa gag cgc gac ttc gac gcc att cgc cct         96
Leu Met Gln Arg Met Val Glu Glu Arg Asp Phe Asp Ala Ile Arg Pro           
            20                  25                  30                    

gtc tac ttt tct act tct cag ctt ggt cag gct gcg ccg tct ttt ggc        144
Val Tyr Phe Ser Thr Ser Gln Leu Gly Gln Ala Ala Pro Ser Phe Gly           
        35                  40                  45                        

gga acc act ggc aca ctt cag gat gcc ttt gat ctg gag gcg cta aag        192
Gly Thr Thr Gly Thr Leu Gln Asp Ala Phe Asp Leu Glu Ala Leu Lys           
    50                  55                  60                            

gcc ctc gat atc att gtg acc tgt cag ggc ggc gat tat acc aac gaa        240
Ala Leu Asp Ile Ile Val Thr Cys Gln Gly Gly Asp Tyr Thr Asn Glu           
65                  70                  75                  80            

atc tat cca aag ctt cgt gaa agc gga tgg cag ggt tac tgg att gac        288
Ile Tyr Pro Lys Leu Arg Glu Ser Gly Trp Gln Gly Tyr Trp Ile Asp           
                85                  90                  95                

gca gca tcg tct ctg cgc atg aaa gat gac gcc atc atc att ctt gac        336
Ala Ala Ser Ser Leu Arg Met Lys Asp Asp Ala Ile Ile Ile Leu Asp           
            100                 105                 110                   

ccc gtc aat cag gac gtc att acc gac gga tta aat aat ggc atc agg        384
Pro Val Asn Gln Asp Val Ile Thr Asp Gly Leu Asn Asn Gly Ile Arg           
        115                 120                 125                       

act ttt gtt ggc ggt aac tgt acc gta agc ctg atg ttg atg tcg ttg        432
Thr Phe Val Gly Gly Asn Cys Thr Val Ser Leu Met Leu Met Ser Leu           
    130                 135                 140                           

ggt ggt tta ttc gcc aat gat ctt gtt gat tgg gtg tcc gtt gca acc        480
Gly Gly Leu Phe Ala Asn Asp Leu Val Asp Trp Val Ser Val Ala Thr           
145                 150                 155                 160           

tac cag gcc gct tcc ggc ggt ggt gcg cga cat atg cgt gag tta tta        528
Tyr Gln Ala Ala Ser Gly Gly Gly Ala Arg His Met Arg Glu Leu Leu           
                165                 170                 175               

acc cag atg ggc cat ctg tat ggc cat gta gca gat gaa ctc gcg acc        576
Thr Gln Met Gly His Leu Tyr Gly His Val Ala Asp Glu Leu Ala Thr           
            180                 185                 190                   

ccg tcc tct gct att ctc gat atc gaa cgc aaa gtc aca acc tta acc        624
Pro Ser Ser Ala Ile Leu Asp Ile Glu Arg Lys Val Thr Thr Leu Thr           
        195                 200                 205                       

cgt agc ggt gag ctg ccg gtg gat aac ttt ggc gtg ccg ctg gcg ggt        672
Arg Ser Gly Glu Leu Pro Val Asp Asn Phe Gly Val Pro Leu Ala Gly           
    210                 215                 220                           

agc ctg att ccg tgg atc gac aaa cag ctc gat aac ggt cag agc cgc        720
Ser Leu Ile Pro Trp Ile Asp Lys Gln Leu Asp Asn Gly Gln Ser Arg           
225                 230                 235                 240           

gaa gag tgg aaa ggg cag gcg gaa acc aac aag atc ctc aac aca tct        768
Glu Glu Trp Lys Gly Gln Ala Glu Thr Asn Lys Ile Leu Asn Thr Ser           
                245                 250                 255               

tcc gta att ccg gta gat ggt tta tgt gtg cgt gtc ggg gca ttg cgc        816
Ser Val Ile Pro Val Asp Gly Leu Cys Val Arg Val Gly Ala Leu Arg           
            260                 265                 270                   

tgc cac agc cag gca ttc act att aaa ttg aaa aaa gat gtg tcg att        864
Cys His Ser Gln Ala Phe Thr Ile Lys Leu Lys Lys Asp Val Ser Ile           
        275                 280                 285                       

ccg acc gtg gaa gaa ctg ctg gct gcg cac aat ccg tgg gcg aaa gtc        912
Pro Thr Val Glu Glu Leu Leu Ala Ala His Asn Pro Trp Ala Lys Val           
    290                 295                 300                           

gtt ccg aac gat cgg gaa atc act atg cgt gag cta acc cca gct gcc        960
Val Pro Asn Asp Arg Glu Ile Thr Met Arg Glu Leu Thr Pro Ala Ala           
305                 310                 315                 320           

gtt acc ggc acg ctg acc acg ccg gta ggc cgc ctg cgt aag ctg aat       1008
Val Thr Gly Thr Leu Thr Thr Pro Val Gly Arg Leu Arg Lys Leu Asn           
                325                 330                 335               

atg gga cca gag ttc ctg tca gcc ttt acc gtg ggc gac cag ctg ctg       1056
Met Gly Pro Glu Phe Leu Ser Ala Phe Thr Val Gly Asp Gln Leu Leu           
            340                 345                 350                   

tgg ggg gcc gcg gag ccg ctg cgt cgg atg ctt cgt caa ctg gcg taa       1104
Trp Gly Ala Ala Glu Pro Leu Arg Arg Met Leu Arg Gln Leu Ala               
        355                 360                 365                       


<210>  8
<211>  367
<212>  PRT
<213>  E. coli

<400>  8

Met Lys Asn Val Gly Phe Ile Gly Trp Arg Gly Met Val Gly Ser Val 
1               5                   10                  15      


Leu Met Gln Arg Met Val Glu Glu Arg Asp Phe Asp Ala Ile Arg Pro 
            20                  25                  30          


Val Tyr Phe Ser Thr Ser Gln Leu Gly Gln Ala Ala Pro Ser Phe Gly 
        35                  40                  45              


Gly Thr Thr Gly Thr Leu Gln Asp Ala Phe Asp Leu Glu Ala Leu Lys 
    50                  55                  60                  


Ala Leu Asp Ile Ile Val Thr Cys Gln Gly Gly Asp Tyr Thr Asn Glu 
65                  70                  75                  80  


Ile Tyr Pro Lys Leu Arg Glu Ser Gly Trp Gln Gly Tyr Trp Ile Asp 
                85                  90                  95      


Ala Ala Ser Ser Leu Arg Met Lys Asp Asp Ala Ile Ile Ile Leu Asp 
            100                 105                 110         


Pro Val Asn Gln Asp Val Ile Thr Asp Gly Leu Asn Asn Gly Ile Arg 
        115                 120                 125             


Thr Phe Val Gly Gly Asn Cys Thr Val Ser Leu Met Leu Met Ser Leu 
    130                 135                 140                 


Gly Gly Leu Phe Ala Asn Asp Leu Val Asp Trp Val Ser Val Ala Thr 
145                 150                 155                 160 


Tyr Gln Ala Ala Ser Gly Gly Gly Ala Arg His Met Arg Glu Leu Leu 
                165                 170                 175     


Thr Gln Met Gly His Leu Tyr Gly His Val Ala Asp Glu Leu Ala Thr 
            180                 185                 190         


Pro Ser Ser Ala Ile Leu Asp Ile Glu Arg Lys Val Thr Thr Leu Thr 
        195                 200                 205             


Arg Ser Gly Glu Leu Pro Val Asp Asn Phe Gly Val Pro Leu Ala Gly 
    210                 215                 220                 


Ser Leu Ile Pro Trp Ile Asp Lys Gln Leu Asp Asn Gly Gln Ser Arg 
225                 230                 235                 240 


Glu Glu Trp Lys Gly Gln Ala Glu Thr Asn Lys Ile Leu Asn Thr Ser 
                245                 250                 255     


Ser Val Ile Pro Val Asp Gly Leu Cys Val Arg Val Gly Ala Leu Arg 
            260                 265                 270         


Cys His Ser Gln Ala Phe Thr Ile Lys Leu Lys Lys Asp Val Ser Ile 
        275                 280                 285             


Pro Thr Val Glu Glu Leu Leu Ala Ala His Asn Pro Trp Ala Lys Val 
    290                 295                 300                 


Val Pro Asn Asp Arg Glu Ile Thr Met Arg Glu Leu Thr Pro Ala Ala 
305                 310                 315                 320 


Val Thr Gly Thr Leu Thr Thr Pro Val Gly Arg Leu Arg Lys Leu Asn 
                325                 330                 335     


Met Gly Pro Glu Phe Leu Ser Ala Phe Thr Val Gly Asp Gln Leu Leu 
            340                 345                 350         


Trp Gly Ala Ala Glu Pro Leu Arg Arg Met Leu Arg Gln Leu Ala 
        355                 360                 365         


<210>  9
<211>  1104
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1104)
<223>  E. coli essential gene thyA

<400>  9
atg aaa aat gtt ggt ttt atc ggc tgg cgc ggt atg gtc ggc tcc gtt         48
Met Lys Asn Val Gly Phe Ile Gly Trp Arg Gly Met Val Gly Ser Val           
1               5                   10                  15                

ctc atg caa cgc atg gtt gaa gag cgc gac ttc gac gcc att cgc cct         96
Leu Met Gln Arg Met Val Glu Glu Arg Asp Phe Asp Ala Ile Arg Pro           
            20                  25                  30                    

gtc tac ttt tct act tct cag ctt ggt cag gct gcg ccg tct ttt ggc        144
Val Tyr Phe Ser Thr Ser Gln Leu Gly Gln Ala Ala Pro Ser Phe Gly           
        35                  40                  45                        

gga acc act ggc aca ctt cag gat gcc ttt gat ctg gag gcg cta aag        192
Gly Thr Thr Gly Thr Leu Gln Asp Ala Phe Asp Leu Glu Ala Leu Lys           
    50                  55                  60                            

gcc ctc gat atc att gtg acc tgt cag ggc ggc gat tat acc aac gaa        240
Ala Leu Asp Ile Ile Val Thr Cys Gln Gly Gly Asp Tyr Thr Asn Glu           
65                  70                  75                  80            

atc tat cca aag ctt cgt gaa agc gga tgg cag ggt tac tgg att gac        288
Ile Tyr Pro Lys Leu Arg Glu Ser Gly Trp Gln Gly Tyr Trp Ile Asp           
                85                  90                  95                

gca gca tcg tct ctg cgc atg aaa gat gac gcc atc atc att ctt gac        336
Ala Ala Ser Ser Leu Arg Met Lys Asp Asp Ala Ile Ile Ile Leu Asp           
            100                 105                 110                   

ccc gtc aat cag gac gtc att acc gac gga tta aat aat ggc atc agg        384
Pro Val Asn Gln Asp Val Ile Thr Asp Gly Leu Asn Asn Gly Ile Arg           
        115                 120                 125                       

act ttt gtt ggc ggt aac tgt acc gta agc ctg atg ttg atg tcg ttg        432
Thr Phe Val Gly Gly Asn Cys Thr Val Ser Leu Met Leu Met Ser Leu           
    130                 135                 140                           

ggt ggt tta ttc gcc aat gat ctt gtt gat tgg gtg tcc gtt gca acc        480
Gly Gly Leu Phe Ala Asn Asp Leu Val Asp Trp Val Ser Val Ala Thr           
145                 150                 155                 160           

tac cag gcc gct tcc ggc ggt ggt gcg cga cat atg cgt gag tta tta        528
Tyr Gln Ala Ala Ser Gly Gly Gly Ala Arg His Met Arg Glu Leu Leu           
                165                 170                 175               

acc cag atg ggc cat ctg tat ggc cat gta gca gat gaa ctc gcg acc        576
Thr Gln Met Gly His Leu Tyr Gly His Val Ala Asp Glu Leu Ala Thr           
            180                 185                 190                   

ccg tcc tct gct att ctc gat atc gaa cgc aaa gtc aca acc tta acc        624
Pro Ser Ser Ala Ile Leu Asp Ile Glu Arg Lys Val Thr Thr Leu Thr           
        195                 200                 205                       

cgt agc ggt gag ctg ccg gtg gat aac ttt ggc gtg ccg ctg gcg ggt        672
Arg Ser Gly Glu Leu Pro Val Asp Asn Phe Gly Val Pro Leu Ala Gly           
    210                 215                 220                           

agc ctg att ccg tgg atc gac aaa cag ctc gat aac ggt cag agc cgc        720
Ser Leu Ile Pro Trp Ile Asp Lys Gln Leu Asp Asn Gly Gln Ser Arg           
225                 230                 235                 240           

gaa gag tgg aaa ggg cag gcg gaa acc aac aag atc ctc aac aca tct        768
Glu Glu Trp Lys Gly Gln Ala Glu Thr Asn Lys Ile Leu Asn Thr Ser           
                245                 250                 255               

tcc gta att ccg gta gat ggt tta tgt gtg cgt gtc ggg gca ttg cgc        816
Ser Val Ile Pro Val Asp Gly Leu Cys Val Arg Val Gly Ala Leu Arg           
            260                 265                 270                   

tgc cac agc cag gca ttc act att aaa ttg aaa aaa gat gtg tcg att        864
Cys His Ser Gln Ala Phe Thr Ile Lys Leu Lys Lys Asp Val Ser Ile           
        275                 280                 285                       

ccg acc gtg gaa gaa ctg ctg gct gcg cac aat ccg tgg gcg aaa gtc        912
Pro Thr Val Glu Glu Leu Leu Ala Ala His Asn Pro Trp Ala Lys Val           
    290                 295                 300                           

gtt ccg aac gat cgg gaa atc act atg cgt gag cta acc cca gct gcc        960
Val Pro Asn Asp Arg Glu Ile Thr Met Arg Glu Leu Thr Pro Ala Ala           
305                 310                 315                 320           

gtt acc ggc acg ctg acc acg ccg gta ggc cgc ctg cgt aag ctg aat       1008
Val Thr Gly Thr Leu Thr Thr Pro Val Gly Arg Leu Arg Lys Leu Asn           
                325                 330                 335               

atg gga cca gag ttc ctg tca gcc ttt acc gtg ggc gac cag ctg ctg       1056
Met Gly Pro Glu Phe Leu Ser Ala Phe Thr Val Gly Asp Gln Leu Leu           
            340                 345                 350                   

tgg ggg gcc gcg gag ccg ctg cgt cgg atg ctt cgt caa ctg gcg taa       1104
Trp Gly Ala Ala Glu Pro Leu Arg Arg Met Leu Arg Gln Leu Ala               
        355                 360                 365                       


<210>  10
<211>  367
<212>  PRT
<213>  E. coli

<400>  10

Met Lys Asn Val Gly Phe Ile Gly Trp Arg Gly Met Val Gly Ser Val 
1               5                   10                  15      


Leu Met Gln Arg Met Val Glu Glu Arg Asp Phe Asp Ala Ile Arg Pro 
            20                  25                  30          


Val Tyr Phe Ser Thr Ser Gln Leu Gly Gln Ala Ala Pro Ser Phe Gly 
        35                  40                  45              


Gly Thr Thr Gly Thr Leu Gln Asp Ala Phe Asp Leu Glu Ala Leu Lys 
    50                  55                  60                  


Ala Leu Asp Ile Ile Val Thr Cys Gln Gly Gly Asp Tyr Thr Asn Glu 
65                  70                  75                  80  


Ile Tyr Pro Lys Leu Arg Glu Ser Gly Trp Gln Gly Tyr Trp Ile Asp 
                85                  90                  95      


Ala Ala Ser Ser Leu Arg Met Lys Asp Asp Ala Ile Ile Ile Leu Asp 
            100                 105                 110         


Pro Val Asn Gln Asp Val Ile Thr Asp Gly Leu Asn Asn Gly Ile Arg 
        115                 120                 125             


Thr Phe Val Gly Gly Asn Cys Thr Val Ser Leu Met Leu Met Ser Leu 
    130                 135                 140                 


Gly Gly Leu Phe Ala Asn Asp Leu Val Asp Trp Val Ser Val Ala Thr 
145                 150                 155                 160 


Tyr Gln Ala Ala Ser Gly Gly Gly Ala Arg His Met Arg Glu Leu Leu 
                165                 170                 175     


Thr Gln Met Gly His Leu Tyr Gly His Val Ala Asp Glu Leu Ala Thr 
            180                 185                 190         


Pro Ser Ser Ala Ile Leu Asp Ile Glu Arg Lys Val Thr Thr Leu Thr 
        195                 200                 205             


Arg Ser Gly Glu Leu Pro Val Asp Asn Phe Gly Val Pro Leu Ala Gly 
    210                 215                 220                 


Ser Leu Ile Pro Trp Ile Asp Lys Gln Leu Asp Asn Gly Gln Ser Arg 
225                 230                 235                 240 


Glu Glu Trp Lys Gly Gln Ala Glu Thr Asn Lys Ile Leu Asn Thr Ser 
                245                 250                 255     


Ser Val Ile Pro Val Asp Gly Leu Cys Val Arg Val Gly Ala Leu Arg 
            260                 265                 270         


Cys His Ser Gln Ala Phe Thr Ile Lys Leu Lys Lys Asp Val Ser Ile 
        275                 280                 285             


Pro Thr Val Glu Glu Leu Leu Ala Ala His Asn Pro Trp Ala Lys Val 
    290                 295                 300                 


Val Pro Asn Asp Arg Glu Ile Thr Met Arg Glu Leu Thr Pro Ala Ala 
305                 310                 315                 320 


Val Thr Gly Thr Leu Thr Thr Pro Val Gly Arg Leu Arg Lys Leu Asn 
                325                 330                 335     


Met Gly Pro Glu Phe Leu Ser Ala Phe Thr Val Gly Asp Gln Leu Leu 
            340                 345                 350         


Trp Gly Ala Ala Glu Pro Leu Arg Arg Met Leu Arg Gln Leu Ala 
        355                 360                 365         


<210>  11
<211>  1842
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1842)
<223>  E. coli essential gene rpoD

<400>  11
atg gag caa aac ccg cag tca cag ctg aaa ctt ctt gtc acc cgt ggt         48
Met Glu Gln Asn Pro Gln Ser Gln Leu Lys Leu Leu Val Thr Arg Gly           
1               5                   10                  15                

aag gag caa ggc tat ctg acc tat gcc gag gtc aat gac cat ctg ccg         96
Lys Glu Gln Gly Tyr Leu Thr Tyr Ala Glu Val Asn Asp His Leu Pro           
            20                  25                  30                    

gaa gat atc gtc gat tca gat cag atc gaa gac atc atc caa atg atc        144
Glu Asp Ile Val Asp Ser Asp Gln Ile Glu Asp Ile Ile Gln Met Ile           
        35                  40                  45                        

aac gac atg ggc att cag gtg atg gaa gaa gca ccg gat gcc gat gat        192
Asn Asp Met Gly Ile Gln Val Met Glu Glu Ala Pro Asp Ala Asp Asp           
    50                  55                  60                            

ctg atg ctg gct gaa aac acc gcg gac gaa gat gct gcc gaa gcc gcc        240
Leu Met Leu Ala Glu Asn Thr Ala Asp Glu Asp Ala Ala Glu Ala Ala           
65                  70                  75                  80            

gcg cag gtg ctt tcc agc gtg gaa tct gaa atc ggg cgc acg act gac        288
Ala Gln Val Leu Ser Ser Val Glu Ser Glu Ile Gly Arg Thr Thr Asp           
                85                  90                  95                

ccg gta cgc atg tac atg cgt gaa atg ggc acc gtt gaa ctg ttg acc        336
Pro Val Arg Met Tyr Met Arg Glu Met Gly Thr Val Glu Leu Leu Thr           
            100                 105                 110                   

cgc gaa ggc gaa att gac atc gct aag cgt att gaa gac ggg atc aac        384
Arg Glu Gly Glu Ile Asp Ile Ala Lys Arg Ile Glu Asp Gly Ile Asn           
        115                 120                 125                       

cag gtt caa tgc tcc gtt gct gaa tat ccg gaa gcg atc acc tat ctg        432
Gln Val Gln Cys Ser Val Ala Glu Tyr Pro Glu Ala Ile Thr Tyr Leu           
    130                 135                 140                           

ctg gaa cag tac gat cgt gtt gaa gca gaa gaa gcg cgt ctg tcc gat        480
Leu Glu Gln Tyr Asp Arg Val Glu Ala Glu Glu Ala Arg Leu Ser Asp           
145                 150                 155                 160           

ctg atc acc ggc ttt gtt gac ccg aac gca gaa gaa gat ctg gca cct        528
Leu Ile Thr Gly Phe Val Asp Pro Asn Ala Glu Glu Asp Leu Ala Pro           
                165                 170                 175               

acc gcc act cac gtc ggt tct gag ctt tcc cag gaa gat ctg gac gat        576
Thr Ala Thr His Val Gly Ser Glu Leu Ser Gln Glu Asp Leu Asp Asp           
            180                 185                 190                   

gac gaa gat gaa gac gaa gaa gat ggc gat gac gac agc gcc gat gat        624
Asp Glu Asp Glu Asp Glu Glu Asp Gly Asp Asp Asp Ser Ala Asp Asp           
        195                 200                 205                       

gac aac agc atc gac ccg gaa ctg gct cgc gaa aaa ttt gcg gaa cta        672
Asp Asn Ser Ile Asp Pro Glu Leu Ala Arg Glu Lys Phe Ala Glu Leu           
    210                 215                 220                           

cgc gct cag tac gtt gta acg cgt gac acc atc aaa gcg aaa ggt cgc        720
Arg Ala Gln Tyr Val Val Thr Arg Asp Thr Ile Lys Ala Lys Gly Arg           
225                 230                 235                 240           

agt cac gct acc gct cag gaa gag atc ctg aaa ctg tct gaa gta ttc        768
Ser His Ala Thr Ala Gln Glu Glu Ile Leu Lys Leu Ser Glu Val Phe           
                245                 250                 255               

aaa cag ttc cgc ctg gtg ccg aag cag ttt gac tac ctg gtc aac agc        816
Lys Gln Phe Arg Leu Val Pro Lys Gln Phe Asp Tyr Leu Val Asn Ser           
            260                 265                 270                   

atg cgc gtc atg atg gac cgc gtt cgt acg caa gaa cgt ctg atc atg        864
Met Arg Val Met Met Asp Arg Val Arg Thr Gln Glu Arg Leu Ile Met           
        275                 280                 285                       

aag ctc tgc gtt gag cag tgc aaa atg ccg aag aaa aac ttc att acc        912
Lys Leu Cys Val Glu Gln Cys Lys Met Pro Lys Lys Asn Phe Ile Thr           
    290                 295                 300                           

ctg ttt acc ggc aac gaa acc agc gat acc tgg ttc aac gcg gca att        960
Leu Phe Thr Gly Asn Glu Thr Ser Asp Thr Trp Phe Asn Ala Ala Ile           
305                 310                 315                 320           

gcg atg aac aag ccg tgg tcg gaa aaa ctg cac gat gtc tct gaa gaa       1008
Ala Met Asn Lys Pro Trp Ser Glu Lys Leu His Asp Val Ser Glu Glu           
                325                 330                 335               

gtg cat cgc gcc ctg caa aaa ctg cag cag att gaa gaa gaa acc ggc       1056
Val His Arg Ala Leu Gln Lys Leu Gln Gln Ile Glu Glu Glu Thr Gly           
            340                 345                 350                   

ctg acc atc gag cag gtt aaa gat atc aac cgt cgt atg tcc atc ggt       1104
Leu Thr Ile Glu Gln Val Lys Asp Ile Asn Arg Arg Met Ser Ile Gly           
        355                 360                 365                       

gaa gcg aaa gcc cgc cgt gcg aag aaa gag atg gtt gaa gcg aac tta       1152
Glu Ala Lys Ala Arg Arg Ala Lys Lys Glu Met Val Glu Ala Asn Leu           
    370                 375                 380                           

cgt ctg gtt att tct atc gct aag aaa tac acc aac cgt ggc ttg cag       1200
Arg Leu Val Ile Ser Ile Ala Lys Lys Tyr Thr Asn Arg Gly Leu Gln           
385                 390                 395                 400           

ttc ctt gac ctg att cag gaa ggc aac atc ggt ctg atg aaa gcg gtt       1248
Phe Leu Asp Leu Ile Gln Glu Gly Asn Ile Gly Leu Met Lys Ala Val           
                405                 410                 415               

gat aaa ttc gaa tac cgc cgt ggt tac aag ttc tcc acc tac gca acc       1296
Asp Lys Phe Glu Tyr Arg Arg Gly Tyr Lys Phe Ser Thr Tyr Ala Thr           
            420                 425                 430                   

tgg tgg atc cgt cag gcg atc acc cgc tct atc gcg gat cag gcg cgc       1344
Trp Trp Ile Arg Gln Ala Ile Thr Arg Ser Ile Ala Asp Gln Ala Arg           
        435                 440                 445                       

acc atc cgt att ccg gtg cat atg att gag acc atc aac aag ctc aac       1392
Thr Ile Arg Ile Pro Val His Met Ile Glu Thr Ile Asn Lys Leu Asn           
    450                 455                 460                           

cgt att tct cgc cag atg ctg caa gag atg ggc cgt gaa ccg acg ccg       1440
Arg Ile Ser Arg Gln Met Leu Gln Glu Met Gly Arg Glu Pro Thr Pro           
465                 470                 475                 480           

gaa gaa ctg gct gaa cgt atg ctg atg ccg gaa gac aag atc cgc aaa       1488
Glu Glu Leu Ala Glu Arg Met Leu Met Pro Glu Asp Lys Ile Arg Lys           
                485                 490                 495               

gtg ctg aag atc gcc aaa gag cca atc tcc atg gaa acg ccg atc ggt       1536
Val Leu Lys Ile Ala Lys Glu Pro Ile Ser Met Glu Thr Pro Ile Gly           
            500                 505                 510                   

gat gat gaa gat tcg cat ctg ggg gat ttc atc gag gat acc acc ctc       1584
Asp Asp Glu Asp Ser His Leu Gly Asp Phe Ile Glu Asp Thr Thr Leu           
        515                 520                 525                       

gag ctg ccg ctg gat tct gcg acc acc gaa agc ctg cgt gcg gca acg       1632
Glu Leu Pro Leu Asp Ser Ala Thr Thr Glu Ser Leu Arg Ala Ala Thr           
    530                 535                 540                           

cac gac gtg ctg gct ggc ctg acc gcg cgt gaa gca aaa gtt ctg cgt       1680
His Asp Val Leu Ala Gly Leu Thr Ala Arg Glu Ala Lys Val Leu Arg           
545                 550                 555                 560           

atg cgt ttc ggt atc gat atg aac acc gac cac acg ctg gaa gaa gtg       1728
Met Arg Phe Gly Ile Asp Met Asn Thr Asp His Thr Leu Glu Glu Val           
                565                 570                 575               

ggt aaa cag ttc gac gtt acc cgc gaa cgt atc cgt cag atc gaa gcg       1776
Gly Lys Gln Phe Asp Val Thr Arg Glu Arg Ile Arg Gln Ile Glu Ala           
            580                 585                 590                   

aag gcg ctg cgc aaa ctg cgt cac ccg agc cgt tct gaa gtg ctg cgt       1824
Lys Ala Leu Arg Lys Leu Arg His Pro Ser Arg Ser Glu Val Leu Arg           
        595                 600                 605                       

agc ttc ctg gac gat taa                                               1842
Ser Phe Leu Asp Asp                                                       
    610                                                                   


<210>  12
<211>  613
<212>  PRT
<213>  E. coli

<400>  12

Met Glu Gln Asn Pro Gln Ser Gln Leu Lys Leu Leu Val Thr Arg Gly 
1               5                   10                  15      


Lys Glu Gln Gly Tyr Leu Thr Tyr Ala Glu Val Asn Asp His Leu Pro 
            20                  25                  30          


Glu Asp Ile Val Asp Ser Asp Gln Ile Glu Asp Ile Ile Gln Met Ile 
        35                  40                  45              


Asn Asp Met Gly Ile Gln Val Met Glu Glu Ala Pro Asp Ala Asp Asp 
    50                  55                  60                  


Leu Met Leu Ala Glu Asn Thr Ala Asp Glu Asp Ala Ala Glu Ala Ala 
65                  70                  75                  80  


Ala Gln Val Leu Ser Ser Val Glu Ser Glu Ile Gly Arg Thr Thr Asp 
                85                  90                  95      


Pro Val Arg Met Tyr Met Arg Glu Met Gly Thr Val Glu Leu Leu Thr 
            100                 105                 110         


Arg Glu Gly Glu Ile Asp Ile Ala Lys Arg Ile Glu Asp Gly Ile Asn 
        115                 120                 125             


Gln Val Gln Cys Ser Val Ala Glu Tyr Pro Glu Ala Ile Thr Tyr Leu 
    130                 135                 140                 


Leu Glu Gln Tyr Asp Arg Val Glu Ala Glu Glu Ala Arg Leu Ser Asp 
145                 150                 155                 160 


Leu Ile Thr Gly Phe Val Asp Pro Asn Ala Glu Glu Asp Leu Ala Pro 
                165                 170                 175     


Thr Ala Thr His Val Gly Ser Glu Leu Ser Gln Glu Asp Leu Asp Asp 
            180                 185                 190         


Asp Glu Asp Glu Asp Glu Glu Asp Gly Asp Asp Asp Ser Ala Asp Asp 
        195                 200                 205             


Asp Asn Ser Ile Asp Pro Glu Leu Ala Arg Glu Lys Phe Ala Glu Leu 
    210                 215                 220                 


Arg Ala Gln Tyr Val Val Thr Arg Asp Thr Ile Lys Ala Lys Gly Arg 
225                 230                 235                 240 


Ser His Ala Thr Ala Gln Glu Glu Ile Leu Lys Leu Ser Glu Val Phe 
                245                 250                 255     


Lys Gln Phe Arg Leu Val Pro Lys Gln Phe Asp Tyr Leu Val Asn Ser 
            260                 265                 270         


Met Arg Val Met Met Asp Arg Val Arg Thr Gln Glu Arg Leu Ile Met 
        275                 280                 285             


Lys Leu Cys Val Glu Gln Cys Lys Met Pro Lys Lys Asn Phe Ile Thr 
    290                 295                 300                 


Leu Phe Thr Gly Asn Glu Thr Ser Asp Thr Trp Phe Asn Ala Ala Ile 
305                 310                 315                 320 


Ala Met Asn Lys Pro Trp Ser Glu Lys Leu His Asp Val Ser Glu Glu 
                325                 330                 335     


Val His Arg Ala Leu Gln Lys Leu Gln Gln Ile Glu Glu Glu Thr Gly 
            340                 345                 350         


Leu Thr Ile Glu Gln Val Lys Asp Ile Asn Arg Arg Met Ser Ile Gly 
        355                 360                 365             


Glu Ala Lys Ala Arg Arg Ala Lys Lys Glu Met Val Glu Ala Asn Leu 
    370                 375                 380                 


Arg Leu Val Ile Ser Ile Ala Lys Lys Tyr Thr Asn Arg Gly Leu Gln 
385                 390                 395                 400 


Phe Leu Asp Leu Ile Gln Glu Gly Asn Ile Gly Leu Met Lys Ala Val 
                405                 410                 415     


Asp Lys Phe Glu Tyr Arg Arg Gly Tyr Lys Phe Ser Thr Tyr Ala Thr 
            420                 425                 430         


Trp Trp Ile Arg Gln Ala Ile Thr Arg Ser Ile Ala Asp Gln Ala Arg 
        435                 440                 445             


Thr Ile Arg Ile Pro Val His Met Ile Glu Thr Ile Asn Lys Leu Asn 
    450                 455                 460                 


Arg Ile Ser Arg Gln Met Leu Gln Glu Met Gly Arg Glu Pro Thr Pro 
465                 470                 475                 480 


Glu Glu Leu Ala Glu Arg Met Leu Met Pro Glu Asp Lys Ile Arg Lys 
                485                 490                 495     


Val Leu Lys Ile Ala Lys Glu Pro Ile Ser Met Glu Thr Pro Ile Gly 
            500                 505                 510         


Asp Asp Glu Asp Ser His Leu Gly Asp Phe Ile Glu Asp Thr Thr Leu 
        515                 520                 525             


Glu Leu Pro Leu Asp Ser Ala Thr Thr Glu Ser Leu Arg Ala Ala Thr 
    530                 535                 540                 


His Asp Val Leu Ala Gly Leu Thr Ala Arg Glu Ala Lys Val Leu Arg 
545                 550                 555                 560 


Met Arg Phe Gly Ile Asp Met Asn Thr Asp His Thr Leu Glu Glu Val 
                565                 570                 575     


Gly Lys Gln Phe Asp Val Thr Arg Glu Arg Ile Arg Gln Ile Glu Ala 
            580                 585                 590         


Lys Ala Leu Arg Lys Leu Arg His Pro Ser Arg Ser Glu Val Leu Arg 
        595                 600                 605             


Ser Phe Leu Asp Asp 
    610             


<210>  13
<211>  546
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(546)
<223>  E. coli essential gene nusG

<400>  13
atg tct gaa gct cct aaa aag cgc tgg tac gtc gtt cag gcg ttt tcc         48
Met Ser Glu Ala Pro Lys Lys Arg Trp Tyr Val Val Gln Ala Phe Ser           
1               5                   10                  15                

ggt ttt gaa ggc cgc gta gca acg tcg ctg cgt gag cat atc aaa tta         96
Gly Phe Glu Gly Arg Val Ala Thr Ser Leu Arg Glu His Ile Lys Leu           
            20                  25                  30                    

cac aac atg gaa gat ttg ttt ggt gaa gtc atg gta cca acc gaa gaa        144
His Asn Met Glu Asp Leu Phe Gly Glu Val Met Val Pro Thr Glu Glu           
        35                  40                  45                        

gtg gtt gaa atc cgt ggc ggt cag cgt cgc aaa agc gaa cgt aaa ttc        192
Val Val Glu Ile Arg Gly Gly Gln Arg Arg Lys Ser Glu Arg Lys Phe           
    50                  55                  60                            

ttc cct ggc tac gtc ctc gtt cag atg gtg atg aac gac gcg agc tgg        240
Phe Pro Gly Tyr Val Leu Val Gln Met Val Met Asn Asp Ala Ser Trp           
65                  70                  75                  80            

cac ctg gtg cgc agc gta ccg cgt gtg atg ggc ttc atc ggc ggt act        288
His Leu Val Arg Ser Val Pro Arg Val Met Gly Phe Ile Gly Gly Thr           
                85                  90                  95                

tcc gat cgt cct gcg cca atc agc gat aaa gaa gtc gat gcg att atg        336
Ser Asp Arg Pro Ala Pro Ile Ser Asp Lys Glu Val Asp Ala Ile Met           
            100                 105                 110                   

aac cgc ctg cag cag gtt ggt gat aag ccg cgt ccg aaa acg ctg ttt        384
Asn Arg Leu Gln Gln Val Gly Asp Lys Pro Arg Pro Lys Thr Leu Phe           
        115                 120                 125                       

gaa ccg ggt gaa atg gtc cgt gtt aat gat ggt ccg ttc gct gac ttc        432
Glu Pro Gly Glu Met Val Arg Val Asn Asp Gly Pro Phe Ala Asp Phe           
    130                 135                 140                           

aac ggt gtt gtt gaa gaa gtg gat tac gag aaa tct cgt ctg aaa gtg        480
Asn Gly Val Val Glu Glu Val Asp Tyr Glu Lys Ser Arg Leu Lys Val           
145                 150                 155                 160           

tct gtt tct atc ttc ggt cgt gcg acc ccg gta gag ctg gac ttc agc        528
Ser Val Ser Ile Phe Gly Arg Ala Thr Pro Val Glu Leu Asp Phe Ser           
                165                 170                 175               

cag gtt gaa aaa gcc taa                                                546
Gln Val Glu Lys Ala                                                       
            180                                                           


<210>  14
<211>  181
<212>  PRT
<213>  E. coli

<400>  14

Met Ser Glu Ala Pro Lys Lys Arg Trp Tyr Val Val Gln Ala Phe Ser 
1               5                   10                  15      


Gly Phe Glu Gly Arg Val Ala Thr Ser Leu Arg Glu His Ile Lys Leu 
            20                  25                  30          


His Asn Met Glu Asp Leu Phe Gly Glu Val Met Val Pro Thr Glu Glu 
        35                  40                  45              


Val Val Glu Ile Arg Gly Gly Gln Arg Arg Lys Ser Glu Arg Lys Phe 
    50                  55                  60                  


Phe Pro Gly Tyr Val Leu Val Gln Met Val Met Asn Asp Ala Ser Trp 
65                  70                  75                  80  


His Leu Val Arg Ser Val Pro Arg Val Met Gly Phe Ile Gly Gly Thr 
                85                  90                  95      


Ser Asp Arg Pro Ala Pro Ile Ser Asp Lys Glu Val Asp Ala Ile Met 
            100                 105                 110         


Asn Arg Leu Gln Gln Val Gly Asp Lys Pro Arg Pro Lys Thr Leu Phe 
        115                 120                 125             


Glu Pro Gly Glu Met Val Arg Val Asn Asp Gly Pro Phe Ala Asp Phe 
    130                 135                 140                 


Asn Gly Val Val Glu Glu Val Asp Tyr Glu Lys Ser Arg Leu Lys Val 
145                 150                 155                 160 


Ser Val Ser Ile Phe Gly Arg Ala Thr Pro Val Glu Leu Asp Phe Ser 
                165                 170                 175     


Gln Val Glu Lys Ala 
            180     


<210>  15
<211>  216
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(216)
<223>  E. coli essential gene rpsU

<400>  15
atg ccg gta att aaa gta cgt gaa aac gag ccg ttc gac gta gct ctg         48
Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu           
1               5                   10                  15                

cgt cgc ttc aag cgt tcc tgc gaa aaa gca ggt gtt ctg gcg gaa gtt         96
Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val           
            20                  25                  30                    

cgt cgt cgt gag ttc tat gaa aaa ccg act acc gaa cgt aag cgc gct        144
Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala           
        35                  40                  45                        

aaa gct tct gca gtg aaa cgt cac gcg aag aaa ctg gct cgc gaa aac        192
Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn           
    50                  55                  60                            

gca cgc cgc act cgt ctg tac taa                                        216
Ala Arg Arg Thr Arg Leu Tyr                                               
65                  70                                                    


<210>  16
<211>  71
<212>  PRT
<213>  E. coli

<400>  16

Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu 
1               5                   10                  15      


Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val 
            20                  25                  30          


Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala 
        35                  40                  45              


Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn 
    50                  55                  60                  


Ala Arg Arg Thr Arg Leu Tyr 
65                  70      


<210>  17
<211>  216
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(216)
<223>  E. coli essential gene accD

<400>  17
atg ccg gta att aaa gta cgt gaa aac gag ccg ttc gac gta gct ctg         48
Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu           
1               5                   10                  15                

cgt cgc ttc aag cgt tcc tgc gaa aaa gca ggt gtt ctg gcg gaa gtt         96
Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val           
            20                  25                  30                    

cgt cgt cgt gag ttc tat gaa aaa ccg act acc gaa cgt aag cgc gct        144
Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala           
        35                  40                  45                        

aaa gct tct gca gtg aaa cgt cac gcg aag aaa ctg gct cgc gaa aac        192
Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn           
    50                  55                  60                            

gca cgc cgc act cgt ctg tac taa                                        216
Ala Arg Arg Thr Arg Leu Tyr                                               
65                  70                                                    


<210>  18
<211>  71
<212>  PRT
<213>  E. coli

<400>  18

Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu 
1               5                   10                  15      


Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val 
            20                  25                  30          


Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala 
        35                  40                  45              


Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn 
    50                  55                  60                  


Ala Arg Arg Thr Arg Leu Tyr 
65                  70      


<210>  19
<211>  216
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(216)
<223>  E coli essential gene degS

<400>  19
atg ccg gta att aaa gta cgt gaa aac gag ccg ttc gac gta gct ctg         48
Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu           
1               5                   10                  15                

cgt cgc ttc aag cgt tcc tgc gaa aaa gca ggt gtt ctg gcg gaa gtt         96
Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val           
            20                  25                  30                    

cgt cgt cgt gag ttc tat gaa aaa ccg act acc gaa cgt aag cgc gct        144
Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala           
        35                  40                  45                        

aaa gct tct gca gtg aaa cgt cac gcg aag aaa ctg gct cgc gaa aac        192
Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn           
    50                  55                  60                            

gca cgc cgc act cgt ctg tac taa                                        216
Ala Arg Arg Thr Arg Leu Tyr                                               
65                  70                                                    


<210>  20
<211>  71
<212>  PRT
<213>  E. coli

<400>  20

Met Pro Val Ile Lys Val Arg Glu Asn Glu Pro Phe Asp Val Ala Leu 
1               5                   10                  15      


Arg Arg Phe Lys Arg Ser Cys Glu Lys Ala Gly Val Leu Ala Glu Val 
            20                  25                  30          


Arg Arg Arg Glu Phe Tyr Glu Lys Pro Thr Thr Glu Arg Lys Arg Ala 
        35                  40                  45              


Lys Ala Ser Ala Val Lys Arg His Ala Lys Lys Leu Ala Arg Glu Asn 
    50                  55                  60                  


Ala Arg Arg Thr Arg Leu Tyr 
65                  70      


<210>  21
<211>  531
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(531)
<223>  E. coli essential gene fldA

<400>  21
atg gct atc act ggc atc ttt ttc ggc agc gac acc ggt aat acc gaa         48
Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly Asn Thr Glu           
1               5                   10                  15                

aat atc gca aaa atg att caa aaa cag ctt ggt aaa gac gtt gcc gat         96
Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp Val Ala Asp           
            20                  25                  30                    

gtc cat gac att gca aaa agc agc aaa gaa gat ctg gaa gct tat gac        144
Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu Ala Tyr Asp           
        35                  40                  45                        

att ctg ctg ctg ggc atc cca acc tgg tat tac ggc gaa gcg cag tgt        192
Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu Ala Gln Cys           
    50                  55                  60                            

gac tgg gat gac ttc ttc ccg act ctc gaa gag att gat ttc aat ggc        240
Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp Phe Asn Gly           
65                  70                  75                  80            

aaa ctg gtt gcg ctg ttt ggt tgt ggt gac cag gaa gat tac gcc gaa        288
Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp Tyr Ala Glu           
                85                  90                  95                

tat ttc tgc gac gca ttg ggc acc atc cgc gac atc att gaa ccg cgc        336
Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile Glu Pro Arg           
            100                 105                 110                   

ggt gca acc atc gtt ggt cac tgg cca act gcg ggc tat cat ttc gaa        384
Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr His Phe Glu           
        115                 120                 125                       

gca tca aaa ggt ctg gca gat gac gac cac ttt gtc ggt ctg gct atc        432
Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly Leu Ala Ile           
    130                 135                 140                           

gac gaa gac cgt cag ccg gaa ctg acc gct gaa cgt gta gaa aaa tgg        480
Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val Glu Lys Trp           
145                 150                 155                 160           

gtt aaa cag att tct gaa gag ttg cat ctc gac gaa att ctc aat gcc        528
Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile Leu Asn Ala           
                165                 170                 175               

tga                                                                    531


<210>  22
<211>  176
<212>  PRT
<213>  E. coli

<400>  22

Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly Asn Thr Glu 
1               5                   10                  15      


Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp Val Ala Asp 
            20                  25                  30          


Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu Ala Tyr Asp 
        35                  40                  45              


Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu Ala Gln Cys 
    50                  55                  60                  


Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp Phe Asn Gly 
65                  70                  75                  80  


Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp Tyr Ala Glu 
                85                  90                  95      


Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile Glu Pro Arg 
            100                 105                 110         


Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr His Phe Glu 
        115                 120                 125             


Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly Leu Ala Ile 
    130                 135                 140                 


Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val Glu Lys Trp 
145                 150                 155                 160 


Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile Leu Asn Ala 
                165                 170                 175     


<210>  23
<211>  960
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(960)
<223>  E. coli essential gene ftsN

<400>  23
gtg gca caa cga gat tat gta cgc cgc agc caa ccg gca cct tcg cgg         48
Val Ala Gln Arg Asp Tyr Val Arg Arg Ser Gln Pro Ala Pro Ser Arg           
1               5                   10                  15                

cga aaa aag agc acc tca cgg aaa aag caa cga aat ctg cct gcg gtt         96
Arg Lys Lys Ser Thr Ser Arg Lys Lys Gln Arg Asn Leu Pro Ala Val           
            20                  25                  30                    

tct ccc gct atg gtc gct att gct gcc gcc gtt ctt gtg acc ttt atc        144
Ser Pro Ala Met Val Ala Ile Ala Ala Ala Val Leu Val Thr Phe Ile           
        35                  40                  45                        

ggt ggt ctg tac ttc att acg cat cac aag aaa gaa gag tcc gag acg        192
Gly Gly Leu Tyr Phe Ile Thr His His Lys Lys Glu Glu Ser Glu Thr           
    50                  55                  60                            

ctg caa agc cag aaa gtg acc gga aac gga cta cca cca aaa cca gaa        240
Leu Gln Ser Gln Lys Val Thr Gly Asn Gly Leu Pro Pro Lys Pro Glu           
65                  70                  75                  80            

gaa cgc tgg cgc tac att aaa gag ctg gaa agt cgc cag ccg gga gtg        288
Glu Arg Trp Arg Tyr Ile Lys Glu Leu Glu Ser Arg Gln Pro Gly Val           
                85                  90                  95                

cgt gcg ccc aca gaa cct tct gcc ggt ggt gaa gtg aaa acg ccg gag        336
Arg Ala Pro Thr Glu Pro Ser Ala Gly Gly Glu Val Lys Thr Pro Glu           
            100                 105                 110                   

caa ctg aca cca gaa caa cgt cag ctt ctt gaa caa atg cag gct gat        384
Gln Leu Thr Pro Glu Gln Arg Gln Leu Leu Glu Gln Met Gln Ala Asp           
        115                 120                 125                       

atg cgc cag cag cca acg cag ctg gtt gaa gtt ccg tgg aat gaa cag        432
Met Arg Gln Gln Pro Thr Gln Leu Val Glu Val Pro Trp Asn Glu Gln           
    130                 135                 140                           

acg ccg gaa cag cgt cag caa acg cta cag cgc caa cgt cag gcg cag        480
Thr Pro Glu Gln Arg Gln Gln Thr Leu Gln Arg Gln Arg Gln Ala Gln           
145                 150                 155                 160           

caa tta gcg gaa cag caa cgc ctg gct cag cag tcg cgt acg act gaa        528
Gln Leu Ala Glu Gln Gln Arg Leu Ala Gln Gln Ser Arg Thr Thr Glu           
                165                 170                 175               

caa agc tgg cag cag cag acg cgt acg tcg caa gcc gca ccg gta cag        576
Gln Ser Trp Gln Gln Gln Thr Arg Thr Ser Gln Ala Ala Pro Val Gln           
            180                 185                 190                   

gcg caa ccg cgc cag tct aaa ccg gct tct tct cag cag ccg tac cag        624
Ala Gln Pro Arg Gln Ser Lys Pro Ala Ser Ser Gln Gln Pro Tyr Gln           
        195                 200                 205                       

gat ctg ctg caa act cct gcg cac acg act gcg cag tcg aag ccg cag        672
Asp Leu Leu Gln Thr Pro Ala His Thr Thr Ala Gln Ser Lys Pro Gln           
    210                 215                 220                           

caa gct gcg cca gtt gct cgt gcc gct gac gcg cca aaa ccg acg gcg        720
Gln Ala Ala Pro Val Ala Arg Ala Ala Asp Ala Pro Lys Pro Thr Ala           
225                 230                 235                 240           

gag aaa aaa gac gaa cgc cgc tgg atg gtg cag tgc ggt tcg ttc aga        768
Glu Lys Lys Asp Glu Arg Arg Trp Met Val Gln Cys Gly Ser Phe Arg           
                245                 250                 255               

ggc gcg gaa cag gca gag acg gta cgt gct cag ctg gcg ttc gaa ggc        816
Gly Ala Glu Gln Ala Glu Thr Val Arg Ala Gln Leu Ala Phe Glu Gly           
            260                 265                 270                   

ttt gac tcg aaa atc acc acc aac aat ggc tgg aat cgt gtg gtc att        864
Phe Asp Ser Lys Ile Thr Thr Asn Asn Gly Trp Asn Arg Val Val Ile           
        275                 280                 285                       

ggc ccg gtg aaa ggc aaa gag aac gca gac agc acc ctc aat cgg ttg        912
Gly Pro Val Lys Gly Lys Glu Asn Ala Asp Ser Thr Leu Asn Arg Leu           
    290                 295                 300                           

aag atg gcg ggc cat aca aac tgc att cgg ctc gcc gcc ggg ggt tga        960
Lys Met Ala Gly His Thr Asn Cys Ile Arg Leu Ala Ala Gly Gly               
305                 310                 315                               


<210>  24
<211>  319
<212>  PRT
<213>  E. coli

<400>  24

Val Ala Gln Arg Asp Tyr Val Arg Arg Ser Gln Pro Ala Pro Ser Arg 
1               5                   10                  15      


Arg Lys Lys Ser Thr Ser Arg Lys Lys Gln Arg Asn Leu Pro Ala Val 
            20                  25                  30          


Ser Pro Ala Met Val Ala Ile Ala Ala Ala Val Leu Val Thr Phe Ile 
        35                  40                  45              


Gly Gly Leu Tyr Phe Ile Thr His His Lys Lys Glu Glu Ser Glu Thr 
    50                  55                  60                  


Leu Gln Ser Gln Lys Val Thr Gly Asn Gly Leu Pro Pro Lys Pro Glu 
65                  70                  75                  80  


Glu Arg Trp Arg Tyr Ile Lys Glu Leu Glu Ser Arg Gln Pro Gly Val 
                85                  90                  95      


Arg Ala Pro Thr Glu Pro Ser Ala Gly Gly Glu Val Lys Thr Pro Glu 
            100                 105                 110         


Gln Leu Thr Pro Glu Gln Arg Gln Leu Leu Glu Gln Met Gln Ala Asp 
        115                 120                 125             


Met Arg Gln Gln Pro Thr Gln Leu Val Glu Val Pro Trp Asn Glu Gln 
    130                 135                 140                 


Thr Pro Glu Gln Arg Gln Gln Thr Leu Gln Arg Gln Arg Gln Ala Gln 
145                 150                 155                 160 


Gln Leu Ala Glu Gln Gln Arg Leu Ala Gln Gln Ser Arg Thr Thr Glu 
                165                 170                 175     


Gln Ser Trp Gln Gln Gln Thr Arg Thr Ser Gln Ala Ala Pro Val Gln 
            180                 185                 190         


Ala Gln Pro Arg Gln Ser Lys Pro Ala Ser Ser Gln Gln Pro Tyr Gln 
        195                 200                 205             


Asp Leu Leu Gln Thr Pro Ala His Thr Thr Ala Gln Ser Lys Pro Gln 
    210                 215                 220                 


Gln Ala Ala Pro Val Ala Arg Ala Ala Asp Ala Pro Lys Pro Thr Ala 
225                 230                 235                 240 


Glu Lys Lys Asp Glu Arg Arg Trp Met Val Gln Cys Gly Ser Phe Arg 
                245                 250                 255     


Gly Ala Glu Gln Ala Glu Thr Val Arg Ala Gln Leu Ala Phe Glu Gly 
            260                 265                 270         


Phe Asp Ser Lys Ile Thr Thr Asn Asn Gly Trp Asn Arg Val Val Ile 
        275                 280                 285             


Gly Pro Val Lys Gly Lys Glu Asn Ala Asp Ser Thr Leu Asn Arg Leu 
    290                 295                 300                 


Lys Met Ala Gly His Thr Asn Cys Ile Arg Leu Ala Ala Gly Gly 
305                 310                 315                 


<210>  25
<211>  1935
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1935)
<223>  E. coli essential gene hflB

<400>  25
atg gcg aaa aac cta ata ctc tgg ctg gtc att gcc gtt gtg ctg atg         48
Met Ala Lys Asn Leu Ile Leu Trp Leu Val Ile Ala Val Val Leu Met           
1               5                   10                  15                

tca gta ttc cag agc ttt ggg ccc agc gag tct aat ggc cgt aag gtg         96
Ser Val Phe Gln Ser Phe Gly Pro Ser Glu Ser Asn Gly Arg Lys Val           
            20                  25                  30                    

gat tac tct acc ttc cta caa gag gtc aat aac gac cag gtt cgt gaa        144
Asp Tyr Ser Thr Phe Leu Gln Glu Val Asn Asn Asp Gln Val Arg Glu           
        35                  40                  45                        

gcg cgt atc aac gga cgt gaa atc aac gtt acc aag aaa gat agt aac        192
Ala Arg Ile Asn Gly Arg Glu Ile Asn Val Thr Lys Lys Asp Ser Asn           
    50                  55                  60                            

cgt tat acc act tac att ccg gtt cag gat ccg aaa tta ctg gat aac        240
Arg Tyr Thr Thr Tyr Ile Pro Val Gln Asp Pro Lys Leu Leu Asp Asn           
65                  70                  75                  80            

ctg ttg acc aag aac gtc aag gtt gtc ggt gaa ccg cct gaa gaa cca        288
Leu Leu Thr Lys Asn Val Lys Val Val Gly Glu Pro Pro Glu Glu Pro           
                85                  90                  95                

agc ctg ctg gct tct atc ttc atc tcc tgg ttc ccg atg ctg ttg ctg        336
Ser Leu Leu Ala Ser Ile Phe Ile Ser Trp Phe Pro Met Leu Leu Leu           
            100                 105                 110                   

att ggt gtc tgg atc ttc ttc atg cgt caa atg cag ggc ggc ggt ggc        384
Ile Gly Val Trp Ile Phe Phe Met Arg Gln Met Gln Gly Gly Gly Gly           
        115                 120                 125                       

aaa ggt gcc atg tcg ttt ggt aag agc aaa gcg cgc atg ctg acg gaa        432
Lys Gly Ala Met Ser Phe Gly Lys Ser Lys Ala Arg Met Leu Thr Glu           
    130                 135                 140                           

gat cag atc aaa acg acc ttt gct gac gtt gcg ggc tgc gac gaa gca        480
Asp Gln Ile Lys Thr Thr Phe Ala Asp Val Ala Gly Cys Asp Glu Ala           
145                 150                 155                 160           

aaa gaa gaa gtt gct gaa ctg gtt gag tat ctg cgc gag ccg agc cgc        528
Lys Glu Glu Val Ala Glu Leu Val Glu Tyr Leu Arg Glu Pro Ser Arg           
                165                 170                 175               

ttc cag aaa ctc ggc ggt aag atc ccg aaa ggc gtc ttg atg gtc ggt        576
Phe Gln Lys Leu Gly Gly Lys Ile Pro Lys Gly Val Leu Met Val Gly           
            180                 185                 190                   

cct ccg ggt acc ggt aaa acg ctg ctg gcg aaa gcg att gca ggc gaa        624
Pro Pro Gly Thr Gly Lys Thr Leu Leu Ala Lys Ala Ile Ala Gly Glu           
        195                 200                 205                       

gcg aaa gtt ccg ttc ttt act atc tcc ggt tct gac ttc gta gaa atg        672
Ala Lys Val Pro Phe Phe Thr Ile Ser Gly Ser Asp Phe Val Glu Met           
    210                 215                 220                           

ttc gtc ggt gtg ggt gca tcc cgt gtt cgt gac atg ttc gaa cag gcg        720
Phe Val Gly Val Gly Ala Ser Arg Val Arg Asp Met Phe Glu Gln Ala           
225                 230                 235                 240           

aag aaa gcg gca ccg tgc atc atc ttt atc gat gaa atc gac gcc gta        768
Lys Lys Ala Ala Pro Cys Ile Ile Phe Ile Asp Glu Ile Asp Ala Val           
                245                 250                 255               

ggc cgc cag cgt ggc gct ggt ctg ggc ggt ggt cac gat gaa cgt gaa        816
Gly Arg Gln Arg Gly Ala Gly Leu Gly Gly Gly His Asp Glu Arg Glu           
            260                 265                 270                   

cag act ctg aac cag atg ctg gtt gag atg gat ggc ttc gaa ggt aac        864
Gln Thr Leu Asn Gln Met Leu Val Glu Met Asp Gly Phe Glu Gly Asn           
        275                 280                 285                       

gaa ggt atc atc gtt atc gcc gcg act aac cgt ccg gac gtt ctc gac        912
Glu Gly Ile Ile Val Ile Ala Ala Thr Asn Arg Pro Asp Val Leu Asp           
    290                 295                 300                           

ccg gcc ctg ctg cgt cct ggc cgt ttc gac cgt cag gtt gtg gtc ggc        960
Pro Ala Leu Leu Arg Pro Gly Arg Phe Asp Arg Gln Val Val Val Gly           
305                 310                 315                 320           

ttg cca gat gtt cgc ggt cgt gag cag atc ctg aaa gtt cac atg cgt       1008
Leu Pro Asp Val Arg Gly Arg Glu Gln Ile Leu Lys Val His Met Arg           
                325                 330                 335               

cgc gta cca ttg gca ccc gat atc gac gcg gca atc att gcc cgt ggt       1056
Arg Val Pro Leu Ala Pro Asp Ile Asp Ala Ala Ile Ile Ala Arg Gly           
            340                 345                 350                   

act cct ggt ttc tcc ggt gct gac ctg gcg aac ctg gtg aac gaa gcg       1104
Thr Pro Gly Phe Ser Gly Ala Asp Leu Ala Asn Leu Val Asn Glu Ala           
        355                 360                 365                       

gca ctg ttc gct gct cgt ggc aac aaa cgc gtt gtg tcg atg gtt gag       1152
Ala Leu Phe Ala Ala Arg Gly Asn Lys Arg Val Val Ser Met Val Glu           
    370                 375                 380                           

ttc gag aaa gcg aaa gac aaa atc atg atg ggt gcg gaa cgt cgc tcc       1200
Phe Glu Lys Ala Lys Asp Lys Ile Met Met Gly Ala Glu Arg Arg Ser           
385                 390                 395                 400           

atg gtg atg acg gaa gcg cag aaa gaa tcg acg gct tac cac gaa gcg       1248
Met Val Met Thr Glu Ala Gln Lys Glu Ser Thr Ala Tyr His Glu Ala           
                405                 410                 415               

ggt cat gcg att atc ggt cgc ctg gtg ccg gaa cac gat ccg gtg cac       1296
Gly His Ala Ile Ile Gly Arg Leu Val Pro Glu His Asp Pro Val His           
            420                 425                 430                   

aaa gtg acg att atc cca cgc ggt cgt gcg ctg ggt gtg act ttc ttc       1344
Lys Val Thr Ile Ile Pro Arg Gly Arg Ala Leu Gly Val Thr Phe Phe           
        435                 440                 445                       

ttg cct gag ggc gac gca atc agc gcc agc cgt cag aaa ctg gaa agc       1392
Leu Pro Glu Gly Asp Ala Ile Ser Ala Ser Arg Gln Lys Leu Glu Ser           
    450                 455                 460                           

cag att tct acg ctg tac ggt ggt cgt ctg gca gaa gag atc atc tac       1440
Gln Ile Ser Thr Leu Tyr Gly Gly Arg Leu Ala Glu Glu Ile Ile Tyr           
465                 470                 475                 480           

ggg ccg gaa cat gta tct acc ggt gcg tcc aac gat att aaa gtt gcg       1488
Gly Pro Glu His Val Ser Thr Gly Ala Ser Asn Asp Ile Lys Val Ala           
                485                 490                 495               

acc aac ctg gca cgt aac atg gtg act cag tgg ggc ttc tct gag aaa       1536
Thr Asn Leu Ala Arg Asn Met Val Thr Gln Trp Gly Phe Ser Glu Lys           
            500                 505                 510                   

ttg ggt cca ctg ctg tac gcg gaa gaa gaa ggt gaa gtg ttc ctc ggc       1584
Leu Gly Pro Leu Leu Tyr Ala Glu Glu Glu Gly Glu Val Phe Leu Gly           
        515                 520                 525                       

cgt agc gta gcg aaa gcg aaa cat atg tcc gat gaa act gca cgt atc       1632
Arg Ser Val Ala Lys Ala Lys His Met Ser Asp Glu Thr Ala Arg Ile           
    530                 535                 540                           

atc gac cag gaa gtg aaa gca ctg att gag cgt aac tat aat cgt gcg       1680
Ile Asp Gln Glu Val Lys Ala Leu Ile Glu Arg Asn Tyr Asn Arg Ala           
545                 550                 555                 560           

cgt cag ctt ctg acc gac aat atg gat att ctg cat gcg atg aaa gat       1728
Arg Gln Leu Leu Thr Asp Asn Met Asp Ile Leu His Ala Met Lys Asp           
                565                 570                 575               

gct ctc atg aaa tat gag act atc gac gca ccg cag att gat gac ctg       1776
Ala Leu Met Lys Tyr Glu Thr Ile Asp Ala Pro Gln Ile Asp Asp Leu           
            580                 585                 590                   

atg gca cgt cgc gat gta cgt ccg cca gcg ggc tgg gaa gaa cca ggc       1824
Met Ala Arg Arg Asp Val Arg Pro Pro Ala Gly Trp Glu Glu Pro Gly           
        595                 600                 605                       

gct tct aac aat tct ggc gac aat ggt agt cca aag gct cct cgt ccg       1872
Ala Ser Asn Asn Ser Gly Asp Asn Gly Ser Pro Lys Ala Pro Arg Pro           
    610                 615                 620                           

gtt gat gaa ccg cgt acg ccg aac ccg ggt aac acc atg tca gag cag       1920
Val Asp Glu Pro Arg Thr Pro Asn Pro Gly Asn Thr Met Ser Glu Gln           
625                 630                 635                 640           

tta ggc gac aag taa                                                   1935
Leu Gly Asp Lys                                                           
                                                                          


<210>  26
<211>  644
<212>  PRT
<213>  E. coli

<400>  26

Met Ala Lys Asn Leu Ile Leu Trp Leu Val Ile Ala Val Val Leu Met 
1               5                   10                  15      


Ser Val Phe Gln Ser Phe Gly Pro Ser Glu Ser Asn Gly Arg Lys Val 
            20                  25                  30          


Asp Tyr Ser Thr Phe Leu Gln Glu Val Asn Asn Asp Gln Val Arg Glu 
        35                  40                  45              


Ala Arg Ile Asn Gly Arg Glu Ile Asn Val Thr Lys Lys Asp Ser Asn 
    50                  55                  60                  


Arg Tyr Thr Thr Tyr Ile Pro Val Gln Asp Pro Lys Leu Leu Asp Asn 
65                  70                  75                  80  


Leu Leu Thr Lys Asn Val Lys Val Val Gly Glu Pro Pro Glu Glu Pro 
                85                  90                  95      


Ser Leu Leu Ala Ser Ile Phe Ile Ser Trp Phe Pro Met Leu Leu Leu 
            100                 105                 110         


Ile Gly Val Trp Ile Phe Phe Met Arg Gln Met Gln Gly Gly Gly Gly 
        115                 120                 125             


Lys Gly Ala Met Ser Phe Gly Lys Ser Lys Ala Arg Met Leu Thr Glu 
    130                 135                 140                 


Asp Gln Ile Lys Thr Thr Phe Ala Asp Val Ala Gly Cys Asp Glu Ala 
145                 150                 155                 160 


Lys Glu Glu Val Ala Glu Leu Val Glu Tyr Leu Arg Glu Pro Ser Arg 
                165                 170                 175     


Phe Gln Lys Leu Gly Gly Lys Ile Pro Lys Gly Val Leu Met Val Gly 
            180                 185                 190         


Pro Pro Gly Thr Gly Lys Thr Leu Leu Ala Lys Ala Ile Ala Gly Glu 
        195                 200                 205             


Ala Lys Val Pro Phe Phe Thr Ile Ser Gly Ser Asp Phe Val Glu Met 
    210                 215                 220                 


Phe Val Gly Val Gly Ala Ser Arg Val Arg Asp Met Phe Glu Gln Ala 
225                 230                 235                 240 


Lys Lys Ala Ala Pro Cys Ile Ile Phe Ile Asp Glu Ile Asp Ala Val 
                245                 250                 255     


Gly Arg Gln Arg Gly Ala Gly Leu Gly Gly Gly His Asp Glu Arg Glu 
            260                 265                 270         


Gln Thr Leu Asn Gln Met Leu Val Glu Met Asp Gly Phe Glu Gly Asn 
        275                 280                 285             


Glu Gly Ile Ile Val Ile Ala Ala Thr Asn Arg Pro Asp Val Leu Asp 
    290                 295                 300                 


Pro Ala Leu Leu Arg Pro Gly Arg Phe Asp Arg Gln Val Val Val Gly 
305                 310                 315                 320 


Leu Pro Asp Val Arg Gly Arg Glu Gln Ile Leu Lys Val His Met Arg 
                325                 330                 335     


Arg Val Pro Leu Ala Pro Asp Ile Asp Ala Ala Ile Ile Ala Arg Gly 
            340                 345                 350         


Thr Pro Gly Phe Ser Gly Ala Asp Leu Ala Asn Leu Val Asn Glu Ala 
        355                 360                 365             


Ala Leu Phe Ala Ala Arg Gly Asn Lys Arg Val Val Ser Met Val Glu 
    370                 375                 380                 


Phe Glu Lys Ala Lys Asp Lys Ile Met Met Gly Ala Glu Arg Arg Ser 
385                 390                 395                 400 


Met Val Met Thr Glu Ala Gln Lys Glu Ser Thr Ala Tyr His Glu Ala 
                405                 410                 415     


Gly His Ala Ile Ile Gly Arg Leu Val Pro Glu His Asp Pro Val His 
            420                 425                 430         


Lys Val Thr Ile Ile Pro Arg Gly Arg Ala Leu Gly Val Thr Phe Phe 
        435                 440                 445             


Leu Pro Glu Gly Asp Ala Ile Ser Ala Ser Arg Gln Lys Leu Glu Ser 
    450                 455                 460                 


Gln Ile Ser Thr Leu Tyr Gly Gly Arg Leu Ala Glu Glu Ile Ile Tyr 
465                 470                 475                 480 


Gly Pro Glu His Val Ser Thr Gly Ala Ser Asn Asp Ile Lys Val Ala 
                485                 490                 495     


Thr Asn Leu Ala Arg Asn Met Val Thr Gln Trp Gly Phe Ser Glu Lys 
            500                 505                 510         


Leu Gly Pro Leu Leu Tyr Ala Glu Glu Glu Gly Glu Val Phe Leu Gly 
        515                 520                 525             


Arg Ser Val Ala Lys Ala Lys His Met Ser Asp Glu Thr Ala Arg Ile 
    530                 535                 540                 


Ile Asp Gln Glu Val Lys Ala Leu Ile Glu Arg Asn Tyr Asn Arg Ala 
545                 550                 555                 560 


Arg Gln Leu Leu Thr Asp Asn Met Asp Ile Leu His Ala Met Lys Asp 
                565                 570                 575     


Ala Leu Met Lys Tyr Glu Thr Ile Asp Ala Pro Gln Ile Asp Asp Leu 
            580                 585                 590         


Met Ala Arg Arg Asp Val Arg Pro Pro Ala Gly Trp Glu Glu Pro Gly 
        595                 600                 605             


Ala Ser Asn Asn Ser Gly Asp Asn Gly Ser Pro Lys Ala Pro Arg Pro 
    610                 615                 620                 


Val Asp Glu Pro Arg Thr Pro Asn Pro Gly Asn Thr Met Ser Glu Gln 
625                 630                 635                 640 


Leu Gly Asp Lys 
                


<210>  27
<211>  612
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(612)
<223>  E. coli essential gene lolA

<400>  27
atg aaa aaa att gcc atc acc tgt gca tta ctc tca agc tta gta gca         48
Met Lys Lys Ile Ala Ile Thr Cys Ala Leu Leu Ser Ser Leu Val Ala           
1               5                   10                  15                

agc agc gtt tgg gct gat gcc gca agc gat ctg aaa agc cgc ctg gat         96
Ser Ser Val Trp Ala Asp Ala Ala Ser Asp Leu Lys Ser Arg Leu Asp           
            20                  25                  30                    

aaa gtc agc agc ttc cac gcc agc ttc aca caa aaa gtg act gac ggt        144
Lys Val Ser Ser Phe His Ala Ser Phe Thr Gln Lys Val Thr Asp Gly           
        35                  40                  45                        

agc ggc gcg gcg gtg cag gaa ggt cag ggc gat ctg tgg gtg aaa cgt        192
Ser Gly Ala Ala Val Gln Glu Gly Gln Gly Asp Leu Trp Val Lys Arg           
    50                  55                  60                            

cca aac tta ttc aac tgg cat atg aca caa cct gat gaa agc att ctg        240
Pro Asn Leu Phe Asn Trp His Met Thr Gln Pro Asp Glu Ser Ile Leu           
65                  70                  75                  80            

gtt tct gac ggt aaa aca ctg tgg ttc tat aac ccg ttc gtt gag caa        288
Val Ser Asp Gly Lys Thr Leu Trp Phe Tyr Asn Pro Phe Val Glu Gln           
                85                  90                  95                

gct acg gca acc tgg ctg aaa gat gcc acc ggt aat acg ccg ttt atg        336
Ala Thr Ala Thr Trp Leu Lys Asp Ala Thr Gly Asn Thr Pro Phe Met           
            100                 105                 110                   

ctg att gcc cgc aac cag tcc agc gac tgg cag cag tac aat atc aaa        384
Leu Ile Ala Arg Asn Gln Ser Ser Asp Trp Gln Gln Tyr Asn Ile Lys           
        115                 120                 125                       

cag aat ggc gat gac ttt gtc ctg acg ccg aaa gcc agc aat ggc aat        432
Gln Asn Gly Asp Asp Phe Val Leu Thr Pro Lys Ala Ser Asn Gly Asn           
    130                 135                 140                           

ctg aag cag ttc acc att aac gtg gga cgt gat ggc aca atc cat cag        480
Leu Lys Gln Phe Thr Ile Asn Val Gly Arg Asp Gly Thr Ile His Gln           
145                 150                 155                 160           

ttt agc gcg gtg gag cag gac gat cag cgc agc agt tat caa ctg aaa        528
Phe Ser Ala Val Glu Gln Asp Asp Gln Arg Ser Ser Tyr Gln Leu Lys           
                165                 170                 175               

tcc cag caa aat ggg gct gtg gat gca gcg aaa ttt acc ttc acc ccg        576
Ser Gln Gln Asn Gly Ala Val Asp Ala Ala Lys Phe Thr Phe Thr Pro           
            180                 185                 190                   

ccg caa ggc gtc acg gta gat gat caa cgt aag tag                        612
Pro Gln Gly Val Thr Val Asp Asp Gln Arg Lys                               
        195                 200                                           


<210>  28
<211>  203
<212>  PRT
<213>  E. coli

<400>  28

Met Lys Lys Ile Ala Ile Thr Cys Ala Leu Leu Ser Ser Leu Val Ala 
1               5                   10                  15      


Ser Ser Val Trp Ala Asp Ala Ala Ser Asp Leu Lys Ser Arg Leu Asp 
            20                  25                  30          


Lys Val Ser Ser Phe His Ala Ser Phe Thr Gln Lys Val Thr Asp Gly 
        35                  40                  45              


Ser Gly Ala Ala Val Gln Glu Gly Gln Gly Asp Leu Trp Val Lys Arg 
    50                  55                  60                  


Pro Asn Leu Phe Asn Trp His Met Thr Gln Pro Asp Glu Ser Ile Leu 
65                  70                  75                  80  


Val Ser Asp Gly Lys Thr Leu Trp Phe Tyr Asn Pro Phe Val Glu Gln 
                85                  90                  95      


Ala Thr Ala Thr Trp Leu Lys Asp Ala Thr Gly Asn Thr Pro Phe Met 
            100                 105                 110         


Leu Ile Ala Arg Asn Gln Ser Ser Asp Trp Gln Gln Tyr Asn Ile Lys 
        115                 120                 125             


Gln Asn Gly Asp Asp Phe Val Leu Thr Pro Lys Ala Ser Asn Gly Asn 
    130                 135                 140                 


Leu Lys Gln Phe Thr Ile Asn Val Gly Arg Asp Gly Thr Ile His Gln 
145                 150                 155                 160 


Phe Ser Ala Val Glu Gln Asp Asp Gln Arg Ser Ser Tyr Gln Leu Lys 
                165                 170                 175     


Ser Gln Gln Asn Gly Ala Val Asp Ala Ala Lys Phe Thr Phe Thr Pro 
            180                 185                 190         


Pro Gln Gly Val Thr Val Asp Asp Gln Arg Lys 
        195                 200             


<210>  29
<211>  1083
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1083)
<223>  E. coli essential gene mraY

<400>  29
atg tta gtt tgg ctg gcc gaa cat ttg gtc aaa tat tat tcc ggc ttt         48
Met Leu Val Trp Leu Ala Glu His Leu Val Lys Tyr Tyr Ser Gly Phe           
1               5                   10                  15                

aac gtc ttt tcc tat ctg acg ttt cgc gcc atc gtc agc ctg ctg acc         96
Asn Val Phe Ser Tyr Leu Thr Phe Arg Ala Ile Val Ser Leu Leu Thr           
            20                  25                  30                    

gcg ctg ttc atc tca ttg tgg atg ggc ccg cgt atg att gct cat ttg        144
Ala Leu Phe Ile Ser Leu Trp Met Gly Pro Arg Met Ile Ala His Leu           
        35                  40                  45                        

caa aaa ctt tcc ttt ggt cag gtg gtg cgt aac gac ggt cct gaa tca        192
Gln Lys Leu Ser Phe Gly Gln Val Val Arg Asn Asp Gly Pro Glu Ser           
    50                  55                  60                            

cac ttc agc aag cgc ggt acg ccg acc atg ggc ggg att atg atc ctg        240
His Phe Ser Lys Arg Gly Thr Pro Thr Met Gly Gly Ile Met Ile Leu           
65                  70                  75                  80            

acg gcg att gtg atc tcc gta ctg ctg tgg gct tac ccg tcc aat ccg        288
Thr Ala Ile Val Ile Ser Val Leu Leu Trp Ala Tyr Pro Ser Asn Pro           
                85                  90                  95                

tac gtc tgg tgc gtg ttg gtg gtg ctg gta ggt tac ggt gtt att ggc        336
Tyr Val Trp Cys Val Leu Val Val Leu Val Gly Tyr Gly Val Ile Gly           
            100                 105                 110                   

ttt gtt gat gat tat cgc aaa gtg gtg cgt aaa gac acc aaa ggg ttg        384
Phe Val Asp Asp Tyr Arg Lys Val Val Arg Lys Asp Thr Lys Gly Leu           
        115                 120                 125                       

atc gct cgt tgg aag tat ttc tgg atg tcg gtc att gcg ctg ggt gtc        432
Ile Ala Arg Trp Lys Tyr Phe Trp Met Ser Val Ile Ala Leu Gly Val           
    130                 135                 140                           

gcc ttc gcc ctg tac ctt gcc ggc aaa gac acg ccc gca acg cag ctg        480
Ala Phe Ala Leu Tyr Leu Ala Gly Lys Asp Thr Pro Ala Thr Gln Leu           
145                 150                 155                 160           

gtg gtc cca ttc ttt aaa gat gtg atg ccg cag ctg ggg ctg ttc tac        528
Val Val Pro Phe Phe Lys Asp Val Met Pro Gln Leu Gly Leu Phe Tyr           
                165                 170                 175               

att ctg ctg gct tac ttc gtc att gtg ggt act ggc aac gcg gta aac        576
Ile Leu Leu Ala Tyr Phe Val Ile Val Gly Thr Gly Asn Ala Val Asn           
            180                 185                 190                   

ctg acc gat ggt ctc gac ggc ctg gca att atg ccg acc gta ttt gtc        624
Leu Thr Asp Gly Leu Asp Gly Leu Ala Ile Met Pro Thr Val Phe Val           
        195                 200                 205                       

gcc ggt ggt ttt gcg ctg gtg gcg tgg gcg acc ggc aat atg aac ttt        672
Ala Gly Gly Phe Ala Leu Val Ala Trp Ala Thr Gly Asn Met Asn Phe           
    210                 215                 220                           

gcc agc tac ttg cat ata ccg tat ctg cga cac gcc ggg gaa ctg gtt        720
Ala Ser Tyr Leu His Ile Pro Tyr Leu Arg His Ala Gly Glu Leu Val           
225                 230                 235                 240           

att gtc tgt acc gcg ata gtc ggg gca gga ctg ggc ttc ctg tgg ttt        768
Ile Val Cys Thr Ala Ile Val Gly Ala Gly Leu Gly Phe Leu Trp Phe           
                245                 250                 255               

aac acc tat ccg gcg cag gtc ttt atg ggc gat gta ggt tcg ctg gcg        816
Asn Thr Tyr Pro Ala Gln Val Phe Met Gly Asp Val Gly Ser Leu Ala           
            260                 265                 270                   

tta ggt ggt gcg tta ggc att atc gcc gta ctg cta cgt cag gaa ttc        864
Leu Gly Gly Ala Leu Gly Ile Ile Ala Val Leu Leu Arg Gln Glu Phe           
        275                 280                 285                       

ctg ctg gtg att atg ggg ggc gtg ttc gtg gta gaa acg ctt tct gtc        912
Leu Leu Val Ile Met Gly Gly Val Phe Val Val Glu Thr Leu Ser Val           
    290                 295                 300                           

atc ctg cag gtc ggc tcc ttt aaa ctg cgc gga caa cgt att ttc cgc        960
Ile Leu Gln Val Gly Ser Phe Lys Leu Arg Gly Gln Arg Ile Phe Arg           
305                 310                 315                 320           

atg gca ccg att cat cac cac tat gaa ctg aaa ggc tgg ccg gaa ccg       1008
Met Ala Pro Ile His His His Tyr Glu Leu Lys Gly Trp Pro Glu Pro           
                325                 330                 335               

cgc gtc att gtg cgt ttc tgg att att tcg ctg atg ctg gtt ctg att       1056
Arg Val Ile Val Arg Phe Trp Ile Ile Ser Leu Met Leu Val Leu Ile           
            340                 345                 350                   

ggt ctg gca acg ctg aag gta cgt taa                                   1083
Gly Leu Ala Thr Leu Lys Val Arg                                           
        355                 360                                           


<210>  30
<211>  360
<212>  PRT
<213>  E. coli

<400>  30

Met Leu Val Trp Leu Ala Glu His Leu Val Lys Tyr Tyr Ser Gly Phe 
1               5                   10                  15      


Asn Val Phe Ser Tyr Leu Thr Phe Arg Ala Ile Val Ser Leu Leu Thr 
            20                  25                  30          


Ala Leu Phe Ile Ser Leu Trp Met Gly Pro Arg Met Ile Ala His Leu 
        35                  40                  45              


Gln Lys Leu Ser Phe Gly Gln Val Val Arg Asn Asp Gly Pro Glu Ser 
    50                  55                  60                  


His Phe Ser Lys Arg Gly Thr Pro Thr Met Gly Gly Ile Met Ile Leu 
65                  70                  75                  80  


Thr Ala Ile Val Ile Ser Val Leu Leu Trp Ala Tyr Pro Ser Asn Pro 
                85                  90                  95      


Tyr Val Trp Cys Val Leu Val Val Leu Val Gly Tyr Gly Val Ile Gly 
            100                 105                 110         


Phe Val Asp Asp Tyr Arg Lys Val Val Arg Lys Asp Thr Lys Gly Leu 
        115                 120                 125             


Ile Ala Arg Trp Lys Tyr Phe Trp Met Ser Val Ile Ala Leu Gly Val 
    130                 135                 140                 


Ala Phe Ala Leu Tyr Leu Ala Gly Lys Asp Thr Pro Ala Thr Gln Leu 
145                 150                 155                 160 


Val Val Pro Phe Phe Lys Asp Val Met Pro Gln Leu Gly Leu Phe Tyr 
                165                 170                 175     


Ile Leu Leu Ala Tyr Phe Val Ile Val Gly Thr Gly Asn Ala Val Asn 
            180                 185                 190         


Leu Thr Asp Gly Leu Asp Gly Leu Ala Ile Met Pro Thr Val Phe Val 
        195                 200                 205             


Ala Gly Gly Phe Ala Leu Val Ala Trp Ala Thr Gly Asn Met Asn Phe 
    210                 215                 220                 


Ala Ser Tyr Leu His Ile Pro Tyr Leu Arg His Ala Gly Glu Leu Val 
225                 230                 235                 240 


Ile Val Cys Thr Ala Ile Val Gly Ala Gly Leu Gly Phe Leu Trp Phe 
                245                 250                 255     


Asn Thr Tyr Pro Ala Gln Val Phe Met Gly Asp Val Gly Ser Leu Ala 
            260                 265                 270         


Leu Gly Gly Ala Leu Gly Ile Ile Ala Val Leu Leu Arg Gln Glu Phe 
        275                 280                 285             


Leu Leu Val Ile Met Gly Gly Val Phe Val Val Glu Thr Leu Ser Val 
    290                 295                 300                 


Ile Leu Gln Val Gly Ser Phe Lys Leu Arg Gly Gln Arg Ile Phe Arg 
305                 310                 315                 320 


Met Ala Pro Ile His His His Tyr Glu Leu Lys Gly Trp Pro Glu Pro 
                325                 330                 335     


Arg Val Ile Val Arg Phe Trp Ile Ile Ser Leu Met Leu Val Leu Ile 
            340                 345                 350         


Gly Leu Ala Thr Leu Lys Val Arg 
        355                 360 


<210>  31
<211>  489
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(489)
<223>  E. coli essential gene mreD

<400>  31
gtg gcg agc tat cgt agc cag gga cgc tgg gta atc tgg ctc tct ttc         48
Val Ala Ser Tyr Arg Ser Gln Gly Arg Trp Val Ile Trp Leu Ser Phe           
1               5                   10                  15                

ctc att gcg ctg ttg ctg caa atc atg ccc tgg ccg gat aac ctg att         96
Leu Ile Ala Leu Leu Leu Gln Ile Met Pro Trp Pro Asp Asn Leu Ile           
            20                  25                  30                    

gtt ttc cgg cca aac tgg gtg tta ctc atc ttg ttg tat tgg atc ctg        144
Val Phe Arg Pro Asn Trp Val Leu Leu Ile Leu Leu Tyr Trp Ile Leu           
        35                  40                  45                        

gcc ttg cct cat cgc gta aat gtg ggc aca ggt ttt gtg atg ggt gcc        192
Ala Leu Pro His Arg Val Asn Val Gly Thr Gly Phe Val Met Gly Ala           
    50                  55                  60                            

ata ctg gat ctg atc agc ggc tcg acg ctt ggc gta cgc gta ttg gcg        240
Ile Leu Asp Leu Ile Ser Gly Ser Thr Leu Gly Val Arg Val Leu Ala           
65                  70                  75                  80            

atg agc atc att gct tac ctg gtg gcg ctg aaa tac cag ctt ttc cgc        288
Met Ser Ile Ile Ala Tyr Leu Val Ala Leu Lys Tyr Gln Leu Phe Arg           
                85                  90                  95                

aac ctc gca tta tgg cag cag gcg ctg gtc gtc atg ttg ctt tcg ctg        336
Asn Leu Ala Leu Trp Gln Gln Ala Leu Val Val Met Leu Leu Ser Leu           
            100                 105                 110                   

gtg gtg gat att att gtt ttc tgg gca gag ttt tta gtg att aac gtc        384
Val Val Asp Ile Ile Val Phe Trp Ala Glu Phe Leu Val Ile Asn Val           
        115                 120                 125                       

tct ttc aga ccg gaa gtg ttc tgg agt agt gta gtc aat ggg gtg ctc        432
Ser Phe Arg Pro Glu Val Phe Trp Ser Ser Val Val Asn Gly Val Leu           
    130                 135                 140                           

tgg ccg tgg att ttc ttg ctg atg cgc aaa gtc cgt cag cag ttt gca        480
Trp Pro Trp Ile Phe Leu Leu Met Arg Lys Val Arg Gln Gln Phe Ala           
145                 150                 155                 160           

gtg caa taa                                                            489
Val Gln                                                                   
                                                                          


<210>  32
<211>  162
<212>  PRT
<213>  E. coli

<400>  32

Val Ala Ser Tyr Arg Ser Gln Gly Arg Trp Val Ile Trp Leu Ser Phe 
1               5                   10                  15      


Leu Ile Ala Leu Leu Leu Gln Ile Met Pro Trp Pro Asp Asn Leu Ile 
            20                  25                  30          


Val Phe Arg Pro Asn Trp Val Leu Leu Ile Leu Leu Tyr Trp Ile Leu 
        35                  40                  45              


Ala Leu Pro His Arg Val Asn Val Gly Thr Gly Phe Val Met Gly Ala 
    50                  55                  60                  


Ile Leu Asp Leu Ile Ser Gly Ser Thr Leu Gly Val Arg Val Leu Ala 
65                  70                  75                  80  


Met Ser Ile Ile Ala Tyr Leu Val Ala Leu Lys Tyr Gln Leu Phe Arg 
                85                  90                  95      


Asn Leu Ala Leu Trp Gln Gln Ala Leu Val Val Met Leu Leu Ser Leu 
            100                 105                 110         


Val Val Asp Ile Ile Val Phe Trp Ala Glu Phe Leu Val Ile Asn Val 
        115                 120                 125             


Ser Phe Arg Pro Glu Val Phe Trp Ser Ser Val Val Asn Gly Val Leu 
    130                 135                 140                 


Trp Pro Trp Ile Phe Leu Leu Met Arg Lys Val Arg Gln Gln Phe Ala 
145                 150                 155                 160 


Val Gln 
        


<210>  33
<211>  1260
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  E. coli essential gene murA

<400>  33
atg gat aaa ttt cgt gtt cag ggg cca acg aag ctc cag ggc gaa gtc         48
Met Asp Lys Phe Arg Val Gln Gly Pro Thr Lys Leu Gln Gly Glu Val           
1               5                   10                  15                

aca att tcc ggc gct aaa aat gct gct ctg cct atc ctt ttt gcc gct         96
Thr Ile Ser Gly Ala Lys Asn Ala Ala Leu Pro Ile Leu Phe Ala Ala           
            20                  25                  30                    

cta ctg gcg gaa gaa ccg gta gag atc cag aac gtc ccg aaa ctg aaa        144
Leu Leu Ala Glu Glu Pro Val Glu Ile Gln Asn Val Pro Lys Leu Lys           
        35                  40                  45                        

gac gtc gat aca tca atg aag ctg cta agc cag ctg ggt gcg aaa gta        192
Asp Val Asp Thr Ser Met Lys Leu Leu Ser Gln Leu Gly Ala Lys Val           
    50                  55                  60                            

gaa cgt aat ggt tct gtg cat att gat gcc cgc gac gtt aat gta ttc        240
Glu Arg Asn Gly Ser Val His Ile Asp Ala Arg Asp Val Asn Val Phe           
65                  70                  75                  80            

tgc gca cct tac gat ctg gtt aaa acc atg cgt gct tct atc tgg gca        288
Cys Ala Pro Tyr Asp Leu Val Lys Thr Met Arg Ala Ser Ile Trp Ala           
                85                  90                  95                

ctg ggg ccg ctg gta gcg cgc ttt ggt cag ggg caa gtt tca cta cct        336
Leu Gly Pro Leu Val Ala Arg Phe Gly Gln Gly Gln Val Ser Leu Pro           
            100                 105                 110                   

ggc ggt tgt acg atc ggt gcg cgt ccg gtt gat cta cac att tct ggc        384
Gly Gly Cys Thr Ile Gly Ala Arg Pro Val Asp Leu His Ile Ser Gly           
        115                 120                 125                       

ctc gaa caa tta ggc gcg acc atc aaa ctg gaa gaa ggt tac gtt aaa        432
Leu Glu Gln Leu Gly Ala Thr Ile Lys Leu Glu Glu Gly Tyr Val Lys           
    130                 135                 140                           

gct tcc gtc gat ggt cgt ctg aaa ggc gca cat atc gtg atg gat aaa        480
Ala Ser Val Asp Gly Arg Leu Lys Gly Ala His Ile Val Met Asp Lys           
145                 150                 155                 160           

gtc agc gtt ggc gca acg gtg acc atc atg tgt gct gca acc ctg gcg        528
Val Ser Val Gly Ala Thr Val Thr Ile Met Cys Ala Ala Thr Leu Ala           
                165                 170                 175               

gaa ggc acc acg att att gaa aac gca gcg cgt gaa ccg gaa atc gtc        576
Glu Gly Thr Thr Ile Ile Glu Asn Ala Ala Arg Glu Pro Glu Ile Val           
            180                 185                 190                   

gat acc gcg aac ttc ctg att acg ctg ggt gcg aaa att agc ggt cag        624
Asp Thr Ala Asn Phe Leu Ile Thr Leu Gly Ala Lys Ile Ser Gly Gln           
        195                 200                 205                       

ggc acc gat cgt atc gtc atc gaa ggt gtg gaa cgt tta ggc ggc ggt        672
Gly Thr Asp Arg Ile Val Ile Glu Gly Val Glu Arg Leu Gly Gly Gly           
    210                 215                 220                           

gtc tat cgc gtg ctg ccg gat cgt atc gaa acc ggt aca ttc ctg gtg        720
Val Tyr Arg Val Leu Pro Asp Arg Ile Glu Thr Gly Thr Phe Leu Val           
225                 230                 235                 240           

gcg gcg gcg att tct cgc ggc aaa att atc tgc cgt aac gcg cag cca        768
Ala Ala Ala Ile Ser Arg Gly Lys Ile Ile Cys Arg Asn Ala Gln Pro           
                245                 250                 255               

gat act ctc gac gcc gtg ctg gcg aaa ctg cgt gac gct gga gcg gac        816
Asp Thr Leu Asp Ala Val Leu Ala Lys Leu Arg Asp Ala Gly Ala Asp           
            260                 265                 270                   

atc gaa gtc ggc gaa gac tgg att agc ctg gat atg cat ggc aaa cgt        864
Ile Glu Val Gly Glu Asp Trp Ile Ser Leu Asp Met His Gly Lys Arg           
        275                 280                 285                       

ccg aag gct gtt aac gta cgt acc gcg ccg cat ccg gca ttc ccg acc        912
Pro Lys Ala Val Asn Val Arg Thr Ala Pro His Pro Ala Phe Pro Thr           
    290                 295                 300                           

gat atg cag gcc cag ttc acg ctg ttg aac ctg gtg gca gaa ggg act        960
Asp Met Gln Ala Gln Phe Thr Leu Leu Asn Leu Val Ala Glu Gly Thr           
305                 310                 315                 320           

gga ttc atc acc gaa acg gtc ttt gaa aac cgc ttt atg cat gtg cca       1008
Gly Phe Ile Thr Glu Thr Val Phe Glu Asn Arg Phe Met His Val Pro           
                325                 330                 335               

gag ctg agc cgt atg ggc gcg cac gcc gaa atc gaa agc aat acc gtt       1056
Glu Leu Ser Arg Met Gly Ala His Ala Glu Ile Glu Ser Asn Thr Val           
            340                 345                 350                   

att tgt cac ggt gtt gaa aaa ctt tct ggc gca cag gtt atg gca acc       1104
Ile Cys His Gly Val Glu Lys Leu Ser Gly Ala Gln Val Met Ala Thr           
        355                 360                 365                       

gat ctg cgt gca tca gca agc ctg gtg ctg gct ggc tgt att gcg gaa       1152
Asp Leu Arg Ala Ser Ala Ser Leu Val Leu Ala Gly Cys Ile Ala Glu           
    370                 375                 380                           

ggg acg acg gtg gtt gat cgt att tat cac atc gat cgt ggc tac gaa       1200
Gly Thr Thr Val Val Asp Arg Ile Tyr His Ile Asp Arg Gly Tyr Glu           
385                 390                 395                 400           

cgc att gaa gac aaa ctg cgc gct tta ggt gca aat att gag cgt gtg       1248
Arg Ile Glu Asp Lys Leu Arg Ala Leu Gly Ala Asn Ile Glu Arg Val           
                405                 410                 415               

aaa ggc gag taa                                                       1260
Lys Gly Glu                                                               
                                                                          


<210>  34
<211>  419
<212>  PRT
<213>  E. coli

<400>  34

Met Asp Lys Phe Arg Val Gln Gly Pro Thr Lys Leu Gln Gly Glu Val 
1               5                   10                  15      


Thr Ile Ser Gly Ala Lys Asn Ala Ala Leu Pro Ile Leu Phe Ala Ala 
            20                  25                  30          


Leu Leu Ala Glu Glu Pro Val Glu Ile Gln Asn Val Pro Lys Leu Lys 
        35                  40                  45              


Asp Val Asp Thr Ser Met Lys Leu Leu Ser Gln Leu Gly Ala Lys Val 
    50                  55                  60                  


Glu Arg Asn Gly Ser Val His Ile Asp Ala Arg Asp Val Asn Val Phe 
65                  70                  75                  80  


Cys Ala Pro Tyr Asp Leu Val Lys Thr Met Arg Ala Ser Ile Trp Ala 
                85                  90                  95      


Leu Gly Pro Leu Val Ala Arg Phe Gly Gln Gly Gln Val Ser Leu Pro 
            100                 105                 110         


Gly Gly Cys Thr Ile Gly Ala Arg Pro Val Asp Leu His Ile Ser Gly 
        115                 120                 125             


Leu Glu Gln Leu Gly Ala Thr Ile Lys Leu Glu Glu Gly Tyr Val Lys 
    130                 135                 140                 


Ala Ser Val Asp Gly Arg Leu Lys Gly Ala His Ile Val Met Asp Lys 
145                 150                 155                 160 


Val Ser Val Gly Ala Thr Val Thr Ile Met Cys Ala Ala Thr Leu Ala 
                165                 170                 175     


Glu Gly Thr Thr Ile Ile Glu Asn Ala Ala Arg Glu Pro Glu Ile Val 
            180                 185                 190         


Asp Thr Ala Asn Phe Leu Ile Thr Leu Gly Ala Lys Ile Ser Gly Gln 
        195                 200                 205             


Gly Thr Asp Arg Ile Val Ile Glu Gly Val Glu Arg Leu Gly Gly Gly 
    210                 215                 220                 


Val Tyr Arg Val Leu Pro Asp Arg Ile Glu Thr Gly Thr Phe Leu Val 
225                 230                 235                 240 


Ala Ala Ala Ile Ser Arg Gly Lys Ile Ile Cys Arg Asn Ala Gln Pro 
                245                 250                 255     


Asp Thr Leu Asp Ala Val Leu Ala Lys Leu Arg Asp Ala Gly Ala Asp 
            260                 265                 270         


Ile Glu Val Gly Glu Asp Trp Ile Ser Leu Asp Met His Gly Lys Arg 
        275                 280                 285             


Pro Lys Ala Val Asn Val Arg Thr Ala Pro His Pro Ala Phe Pro Thr 
    290                 295                 300                 


Asp Met Gln Ala Gln Phe Thr Leu Leu Asn Leu Val Ala Glu Gly Thr 
305                 310                 315                 320 


Gly Phe Ile Thr Glu Thr Val Phe Glu Asn Arg Phe Met His Val Pro 
                325                 330                 335     


Glu Leu Ser Arg Met Gly Ala His Ala Glu Ile Glu Ser Asn Thr Val 
            340                 345                 350         


Ile Cys His Gly Val Glu Lys Leu Ser Gly Ala Gln Val Met Ala Thr 
        355                 360                 365             


Asp Leu Arg Ala Ser Ala Ser Leu Val Leu Ala Gly Cys Ile Ala Glu 
    370                 375                 380                 


Gly Thr Thr Val Val Asp Arg Ile Tyr His Ile Asp Arg Gly Tyr Glu 
385                 390                 395                 400 


Arg Ile Glu Asp Lys Leu Arg Ala Leu Gly Ala Asn Ile Glu Arg Val 
                405                 410                 415     


Lys Gly Glu 
            


<210>  35
<211>  1029
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1029)
<223>  E. coli essential gene murB

<400>  35
atg aac cac tcc tta aaa ccc tgg aac aca ttt ggc att gat cat aat         48
Met Asn His Ser Leu Lys Pro Trp Asn Thr Phe Gly Ile Asp His Asn           
1               5                   10                  15                

gct cag cac att gta tgt gcc gaa gac gaa caa caa tta ctc aat gcc         96
Ala Gln His Ile Val Cys Ala Glu Asp Glu Gln Gln Leu Leu Asn Ala           
            20                  25                  30                    

tgg cag cat gca acc gca gaa gga caa ccc gtt ctt att ctg ggt gaa        144
Trp Gln His Ala Thr Ala Glu Gly Gln Pro Val Leu Ile Leu Gly Glu           
        35                  40                  45                        

gga agt aat gta ctt ttt ctg gaa gac tat cgc ggc acg gtg atc atc        192
Gly Ser Asn Val Leu Phe Leu Glu Asp Tyr Arg Gly Thr Val Ile Ile           
    50                  55                  60                            

aac cgg atc aaa ggt atc gaa att cat gat gaa cct gat gcg tgg tat        240
Asn Arg Ile Lys Gly Ile Glu Ile His Asp Glu Pro Asp Ala Trp Tyr           
65                  70                  75                  80            

tta cat gta gga gcc gga gaa aac tgg cat cgc ctg gta aaa tac act        288
Leu His Val Gly Ala Gly Glu Asn Trp His Arg Leu Val Lys Tyr Thr           
                85                  90                  95                

ttg cag gaa ggt atg cct ggt ctg gaa aat ctg gca tta att cct ggt        336
Leu Gln Glu Gly Met Pro Gly Leu Glu Asn Leu Ala Leu Ile Pro Gly           
            100                 105                 110                   

tgt gtc ggc tca tca cct atc cag aat att ggt gct tat ggc gta gaa        384
Cys Val Gly Ser Ser Pro Ile Gln Asn Ile Gly Ala Tyr Gly Val Glu           
        115                 120                 125                       

tta cag cga gtt tgc gct tat gtt gat tgt gtt gaa ctg gcg aca ggc        432
Leu Gln Arg Val Cys Ala Tyr Val Asp Cys Val Glu Leu Ala Thr Gly           
    130                 135                 140                           

aag caa gtg cgc tta act gcc aaa gag tgc cgt ttt ggc tat cgc gac        480
Lys Gln Val Arg Leu Thr Ala Lys Glu Cys Arg Phe Gly Tyr Arg Asp           
145                 150                 155                 160           

agt att ttt aaa cat gaa tac cag gat cgc ttc gcc att gta gcc gta        528
Ser Ile Phe Lys His Glu Tyr Gln Asp Arg Phe Ala Ile Val Ala Val           
                165                 170                 175               

ggt ctg cgt ctg cca aaa gag tgg caa cct gta cta acg tat ggt gac        576
Gly Leu Arg Leu Pro Lys Glu Trp Gln Pro Val Leu Thr Tyr Gly Asp           
            180                 185                 190                   

tta act cgt ctg gat cct aca aca gta acg cca cag caa gta ttt aat        624
Leu Thr Arg Leu Asp Pro Thr Thr Val Thr Pro Gln Gln Val Phe Asn           
        195                 200                 205                       

gcg gtg tgt cat atg cgc acc acc aaa ctc cct gat cca aaa gtg aat        672
Ala Val Cys His Met Arg Thr Thr Lys Leu Pro Asp Pro Lys Val Asn           
    210                 215                 220                           

ggc aat gcc ggt agt ttc ttc aaa aac cct gtt gta tct gcc gaa acg        720
Gly Asn Ala Gly Ser Phe Phe Lys Asn Pro Val Val Ser Ala Glu Thr           
225                 230                 235                 240           

gct gaa gca tta ctg tca caa ttt cca aca gca cca aat tac ccc cag        768
Ala Glu Ala Leu Leu Ser Gln Phe Pro Thr Ala Pro Asn Tyr Pro Gln           
                245                 250                 255               

gcg gat ggt tca gta aaa ctg gca gca ggt tgg ctt att gat cag tgc        816
Ala Asp Gly Ser Val Lys Leu Ala Ala Gly Trp Leu Ile Asp Gln Cys           
            260                 265                 270                   

cag cta aaa ggg atg caa atg ggt ggg gct gcg gtg cac cgt caa cag        864
Gln Leu Lys Gly Met Gln Met Gly Gly Ala Ala Val His Arg Gln Gln           
        275                 280                 285                       

gcg tta gtt ctc att aat gaa gac aat gca aaa agc gaa gat gtg gtg        912
Ala Leu Val Leu Ile Asn Glu Asp Asn Ala Lys Ser Glu Asp Val Val           
    290                 295                 300                           

caa ctg gca cac cat gta aga caa aaa gtg ggt gaa aaa ttt aat gtc        960
Gln Leu Ala His His Val Arg Gln Lys Val Gly Glu Lys Phe Asn Val           
305                 310                 315                 320           

tgg ctt gag cct gaa gtc cgc ttt att ggt gca tca ggt gaa gtg agc       1008
Trp Leu Glu Pro Glu Val Arg Phe Ile Gly Ala Ser Gly Glu Val Ser           
                325                 330                 335               

gca gtg gag aca att tca tga                                           1029
Ala Val Glu Thr Ile Ser                                                   
            340                                                           


<210>  36
<211>  342
<212>  PRT
<213>  E. coli

<400>  36

Met Asn His Ser Leu Lys Pro Trp Asn Thr Phe Gly Ile Asp His Asn 
1               5                   10                  15      


Ala Gln His Ile Val Cys Ala Glu Asp Glu Gln Gln Leu Leu Asn Ala 
            20                  25                  30          


Trp Gln His Ala Thr Ala Glu Gly Gln Pro Val Leu Ile Leu Gly Glu 
        35                  40                  45              


Gly Ser Asn Val Leu Phe Leu Glu Asp Tyr Arg Gly Thr Val Ile Ile 
    50                  55                  60                  


Asn Arg Ile Lys Gly Ile Glu Ile His Asp Glu Pro Asp Ala Trp Tyr 
65                  70                  75                  80  


Leu His Val Gly Ala Gly Glu Asn Trp His Arg Leu Val Lys Tyr Thr 
                85                  90                  95      


Leu Gln Glu Gly Met Pro Gly Leu Glu Asn Leu Ala Leu Ile Pro Gly 
            100                 105                 110         


Cys Val Gly Ser Ser Pro Ile Gln Asn Ile Gly Ala Tyr Gly Val Glu 
        115                 120                 125             


Leu Gln Arg Val Cys Ala Tyr Val Asp Cys Val Glu Leu Ala Thr Gly 
    130                 135                 140                 


Lys Gln Val Arg Leu Thr Ala Lys Glu Cys Arg Phe Gly Tyr Arg Asp 
145                 150                 155                 160 


Ser Ile Phe Lys His Glu Tyr Gln Asp Arg Phe Ala Ile Val Ala Val 
                165                 170                 175     


Gly Leu Arg Leu Pro Lys Glu Trp Gln Pro Val Leu Thr Tyr Gly Asp 
            180                 185                 190         


Leu Thr Arg Leu Asp Pro Thr Thr Val Thr Pro Gln Gln Val Phe Asn 
        195                 200                 205             


Ala Val Cys His Met Arg Thr Thr Lys Leu Pro Asp Pro Lys Val Asn 
    210                 215                 220                 


Gly Asn Ala Gly Ser Phe Phe Lys Asn Pro Val Val Ser Ala Glu Thr 
225                 230                 235                 240 


Ala Glu Ala Leu Leu Ser Gln Phe Pro Thr Ala Pro Asn Tyr Pro Gln 
                245                 250                 255     


Ala Asp Gly Ser Val Lys Leu Ala Ala Gly Trp Leu Ile Asp Gln Cys 
            260                 265                 270         


Gln Leu Lys Gly Met Gln Met Gly Gly Ala Ala Val His Arg Gln Gln 
        275                 280                 285             


Ala Leu Val Leu Ile Asn Glu Asp Asn Ala Lys Ser Glu Asp Val Val 
    290                 295                 300                 


Gln Leu Ala His His Val Arg Gln Lys Val Gly Glu Lys Phe Asn Val 
305                 310                 315                 320 


Trp Leu Glu Pro Glu Val Arg Phe Ile Gly Ala Ser Gly Glu Val Ser 
                325                 330                 335     


Ala Val Glu Thr Ile Ser 
            340         


<210>  37
<211>  1359
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1359)
<223>  E. coli essential gene murF

<400>  37
atg att agc gta acc ctt agc caa ctt acc gac att ctc aac ggt gaa         48
Met Ile Ser Val Thr Leu Ser Gln Leu Thr Asp Ile Leu Asn Gly Glu           
1               5                   10                  15                

ctg caa ggt gca gat atc acc ctt gat gct gta acc act gat acc cga         96
Leu Gln Gly Ala Asp Ile Thr Leu Asp Ala Val Thr Thr Asp Thr Arg           
            20                  25                  30                    

aaa ctg acg ccg ggc tgc ctg ttt gtt gcc ctg aaa ggc gaa cgt ttt        144
Lys Leu Thr Pro Gly Cys Leu Phe Val Ala Leu Lys Gly Glu Arg Phe           
        35                  40                  45                        

gat gcc cac gat ttt gcc gac cag gcg aaa gct ggc ggc gca ggc gca        192
Asp Ala His Asp Phe Ala Asp Gln Ala Lys Ala Gly Gly Ala Gly Ala           
    50                  55                  60                            

cta ctg gtt agc cgt ccg ctg gac atc gac ctg ccg cag tta atc gtc        240
Leu Leu Val Ser Arg Pro Leu Asp Ile Asp Leu Pro Gln Leu Ile Val           
65                  70                  75                  80            

aag gat acg cgt ctg gcg ttt ggt gaa ctg gct gca tgg gtt cgc cag        288
Lys Asp Thr Arg Leu Ala Phe Gly Glu Leu Ala Ala Trp Val Arg Gln           
                85                  90                  95                

caa gtt ccg gcg cgc gtg gtt gct ctg acg ggg tcc tcc ggc aaa acc        336
Gln Val Pro Ala Arg Val Val Ala Leu Thr Gly Ser Ser Gly Lys Thr           
            100                 105                 110                   

tcc gtt aaa gag atg acg gcg gcg att tta agc cag tgc ggc aac acg        384
Ser Val Lys Glu Met Thr Ala Ala Ile Leu Ser Gln Cys Gly Asn Thr           
        115                 120                 125                       

ctt tat acg gca ggc aat ctc aac aac gac atc ggt gta ccg atg acg        432
Leu Tyr Thr Ala Gly Asn Leu Asn Asn Asp Ile Gly Val Pro Met Thr           
    130                 135                 140                           

ctg ttg cgc tta acg ccg gaa tac gat tac gca gtt att gaa ctt ggc        480
Leu Leu Arg Leu Thr Pro Glu Tyr Asp Tyr Ala Val Ile Glu Leu Gly           
145                 150                 155                 160           

gcg aac cat cag ggc gaa ata gcc tgg act gtg agt ctg act cgc ccg        528
Ala Asn His Gln Gly Glu Ile Ala Trp Thr Val Ser Leu Thr Arg Pro           
                165                 170                 175               

gaa gct gcg ctg gtc aac aac ctg gca gcg gcg cat ctg gaa ggt ttt        576
Glu Ala Ala Leu Val Asn Asn Leu Ala Ala Ala His Leu Glu Gly Phe           
            180                 185                 190                   

ggc tcg ctt gcg ggt gtc gcg aaa gcg aaa ggt gaa atc ttt agc ggc        624
Gly Ser Leu Ala Gly Val Ala Lys Ala Lys Gly Glu Ile Phe Ser Gly           
        195                 200                 205                       

ctg ccg gaa aac ggt atc gcc att atg aac gcc gac aac aac gac tgg        672
Leu Pro Glu Asn Gly Ile Ala Ile Met Asn Ala Asp Asn Asn Asp Trp           
    210                 215                 220                           

ctg aac tgg cag agc gta att ggc tca cgc aaa gtg tgg cgt ttc tca        720
Leu Asn Trp Gln Ser Val Ile Gly Ser Arg Lys Val Trp Arg Phe Ser           
225                 230                 235                 240           

ccc aat gcc gcc aac agc gat ttc acc gcc acc aat atc cat gtg acc        768
Pro Asn Ala Ala Asn Ser Asp Phe Thr Ala Thr Asn Ile His Val Thr           
                245                 250                 255               

tcg cac ggt acg gaa ttt acc cta caa acc cca acc ggt agc gtc gat        816
Ser His Gly Thr Glu Phe Thr Leu Gln Thr Pro Thr Gly Ser Val Asp           
            260                 265                 270                   

gtt ctg ctg ccg ttg ccg ggg cgt cac aat att gcg aat gcg ctg gca        864
Val Leu Leu Pro Leu Pro Gly Arg His Asn Ile Ala Asn Ala Leu Ala           
        275                 280                 285                       

gcc gct gcg ctc tcc atg tcc gtg ggc gca acg ctt gat gct atc aaa        912
Ala Ala Ala Leu Ser Met Ser Val Gly Ala Thr Leu Asp Ala Ile Lys           
    290                 295                 300                           

gcg ggg ctg gca aat ctg aaa gct gtt cca ggc cgt ctg ttc ccc atc        960
Ala Gly Leu Ala Asn Leu Lys Ala Val Pro Gly Arg Leu Phe Pro Ile           
305                 310                 315                 320           

caa ctg gca gaa aac cag ttg ctg ctc gac gac tcc tac aac gcc aat       1008
Gln Leu Ala Glu Asn Gln Leu Leu Leu Asp Asp Ser Tyr Asn Ala Asn           
                325                 330                 335               

gtc ggt tca atg act gca gca gtc cag gta ctg gct gaa atg ccg ggc       1056
Val Gly Ser Met Thr Ala Ala Val Gln Val Leu Ala Glu Met Pro Gly           
            340                 345                 350                   

tac cgc gtg ctg gtg gtg ggc gat atg gcg gaa ctg ggc gct gaa agc       1104
Tyr Arg Val Leu Val Val Gly Asp Met Ala Glu Leu Gly Ala Glu Ser           
        355                 360                 365                       

gaa gcc tgc cat gta cag gtg ggc gag gcg gca aaa gct gct ggt att       1152
Glu Ala Cys His Val Gln Val Gly Glu Ala Ala Lys Ala Ala Gly Ile           
    370                 375                 380                           

gac cgc gtg tta agc gtg ggt aaa caa agc cat gct atc agc acc gcc       1200
Asp Arg Val Leu Ser Val Gly Lys Gln Ser His Ala Ile Ser Thr Ala           
385                 390                 395                 400           

agc ggc gtt ggc gaa cat ttt gct gat aaa act gcg tta att acg cgt       1248
Ser Gly Val Gly Glu His Phe Ala Asp Lys Thr Ala Leu Ile Thr Arg           
                405                 410                 415               

ctt aaa tca ctg att gct gag caa cag gta att acg att tta gtt aag       1296
Leu Lys Ser Leu Ile Ala Glu Gln Gln Val Ile Thr Ile Leu Val Lys           
            420                 425                 430                   

ggt tca cgt agt gcc gcc atg gaa gag gta gta cgc gct tta cag gag       1344
Gly Ser Arg Ser Ala Ala Met Glu Glu Val Val Arg Ala Leu Gln Glu           
        435                 440                 445                       

aat ggg aca tgt tag                                                   1359
Asn Gly Thr Cys                                                           
    450                                                                   


<210>  38
<211>  452
<212>  PRT
<213>  E. coli

<400>  38

Met Ile Ser Val Thr Leu Ser Gln Leu Thr Asp Ile Leu Asn Gly Glu 
1               5                   10                  15      


Leu Gln Gly Ala Asp Ile Thr Leu Asp Ala Val Thr Thr Asp Thr Arg 
            20                  25                  30          


Lys Leu Thr Pro Gly Cys Leu Phe Val Ala Leu Lys Gly Glu Arg Phe 
        35                  40                  45              


Asp Ala His Asp Phe Ala Asp Gln Ala Lys Ala Gly Gly Ala Gly Ala 
    50                  55                  60                  


Leu Leu Val Ser Arg Pro Leu Asp Ile Asp Leu Pro Gln Leu Ile Val 
65                  70                  75                  80  


Lys Asp Thr Arg Leu Ala Phe Gly Glu Leu Ala Ala Trp Val Arg Gln 
                85                  90                  95      


Gln Val Pro Ala Arg Val Val Ala Leu Thr Gly Ser Ser Gly Lys Thr 
            100                 105                 110         


Ser Val Lys Glu Met Thr Ala Ala Ile Leu Ser Gln Cys Gly Asn Thr 
        115                 120                 125             


Leu Tyr Thr Ala Gly Asn Leu Asn Asn Asp Ile Gly Val Pro Met Thr 
    130                 135                 140                 


Leu Leu Arg Leu Thr Pro Glu Tyr Asp Tyr Ala Val Ile Glu Leu Gly 
145                 150                 155                 160 


Ala Asn His Gln Gly Glu Ile Ala Trp Thr Val Ser Leu Thr Arg Pro 
                165                 170                 175     


Glu Ala Ala Leu Val Asn Asn Leu Ala Ala Ala His Leu Glu Gly Phe 
            180                 185                 190         


Gly Ser Leu Ala Gly Val Ala Lys Ala Lys Gly Glu Ile Phe Ser Gly 
        195                 200                 205             


Leu Pro Glu Asn Gly Ile Ala Ile Met Asn Ala Asp Asn Asn Asp Trp 
    210                 215                 220                 


Leu Asn Trp Gln Ser Val Ile Gly Ser Arg Lys Val Trp Arg Phe Ser 
225                 230                 235                 240 


Pro Asn Ala Ala Asn Ser Asp Phe Thr Ala Thr Asn Ile His Val Thr 
                245                 250                 255     


Ser His Gly Thr Glu Phe Thr Leu Gln Thr Pro Thr Gly Ser Val Asp 
            260                 265                 270         


Val Leu Leu Pro Leu Pro Gly Arg His Asn Ile Ala Asn Ala Leu Ala 
        275                 280                 285             


Ala Ala Ala Leu Ser Met Ser Val Gly Ala Thr Leu Asp Ala Ile Lys 
    290                 295                 300                 


Ala Gly Leu Ala Asn Leu Lys Ala Val Pro Gly Arg Leu Phe Pro Ile 
305                 310                 315                 320 


Gln Leu Ala Glu Asn Gln Leu Leu Leu Asp Asp Ser Tyr Asn Ala Asn 
                325                 330                 335     


Val Gly Ser Met Thr Ala Ala Val Gln Val Leu Ala Glu Met Pro Gly 
            340                 345                 350         


Tyr Arg Val Leu Val Val Gly Asp Met Ala Glu Leu Gly Ala Glu Ser 
        355                 360                 365             


Glu Ala Cys His Val Gln Val Gly Glu Ala Ala Lys Ala Ala Gly Ile 
    370                 375                 380                 


Asp Arg Val Leu Ser Val Gly Lys Gln Ser His Ala Ile Ser Thr Ala 
385                 390                 395                 400 


Ser Gly Val Gly Glu His Phe Ala Asp Lys Thr Ala Leu Ile Thr Arg 
                405                 410                 415     


Leu Lys Ser Leu Ile Ala Glu Gln Gln Val Ile Thr Ile Leu Val Lys 
            420                 425                 430         


Gly Ser Arg Ser Ala Ala Met Glu Glu Val Val Arg Ala Leu Gln Glu 
        435                 440                 445             


Asn Gly Thr Cys 
    450         


<210>  39
<211>  642
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(642)
<223>  E. coli essential gene nadD

<400>  39
atg aaa tct tta cag gct ctg ttt ggc ggc acc ttt gat ccg gtg cac         48
Met Lys Ser Leu Gln Ala Leu Phe Gly Gly Thr Phe Asp Pro Val His           
1               5                   10                  15                

tat ggt cat cta aaa ccc gtg gaa acg ctg gcg aat ttg att ggt ctg         96
Tyr Gly His Leu Lys Pro Val Glu Thr Leu Ala Asn Leu Ile Gly Leu           
            20                  25                  30                    

acg cgg gtc aca atc atc cct aat aat gtt cct ccg cat cgt ccc cag        144
Thr Arg Val Thr Ile Ile Pro Asn Asn Val Pro Pro His Arg Pro Gln           
        35                  40                  45                        

ccg gaa gcg aac agc gtg cag cgt aaa cac atg ctt gaa ctg gcg att        192
Pro Glu Ala Asn Ser Val Gln Arg Lys His Met Leu Glu Leu Ala Ile           
    50                  55                  60                            

gcc gac aag cca tta ttt act ctt gat gaa cgc gag cta aag cgc aat        240
Ala Asp Lys Pro Leu Phe Thr Leu Asp Glu Arg Glu Leu Lys Arg Asn           
65                  70                  75                  80            

gcc ccc tct tac act gcg caa aca ctg aaa gag tgg cgg cag gaa caa        288
Ala Pro Ser Tyr Thr Ala Gln Thr Leu Lys Glu Trp Arg Gln Glu Gln           
                85                  90                  95                

gga ccg gac gtg ccg ctg gcg ttt att att ggt cag gat tca ttg ctg        336
Gly Pro Asp Val Pro Leu Ala Phe Ile Ile Gly Gln Asp Ser Leu Leu           
            100                 105                 110                   

acc ttt ccg acc tgg tac gaa tac gaa acg ata ctc gac aat gca cat        384
Thr Phe Pro Thr Trp Tyr Glu Tyr Glu Thr Ile Leu Asp Asn Ala His           
        115                 120                 125                       

ttg atc gtc tgt cgg cgt cca ggt tac cca ctt gaa atg gcg caa ccg        432
Leu Ile Val Cys Arg Arg Pro Gly Tyr Pro Leu Glu Met Ala Gln Pro           
    130                 135                 140                           

caa tac cag caa tgg ctg gaa gat cat ttg aca cat aac ccg gaa gat        480
Gln Tyr Gln Gln Trp Leu Glu Asp His Leu Thr His Asn Pro Glu Asp           
145                 150                 155                 160           

ctt cac ctt cag cct gcc ggt aaa att tat ctg gct gaa acg ccg tgg        528
Leu His Leu Gln Pro Ala Gly Lys Ile Tyr Leu Ala Glu Thr Pro Trp           
                165                 170                 175               

ttt aac atc tcg gcg acc atc atc cgc gaa cgt ttg caa aac ggt gaa        576
Phe Asn Ile Ser Ala Thr Ile Ile Arg Glu Arg Leu Gln Asn Gly Glu           
            180                 185                 190                   

tcg tgt gag gat tta ttg ccg gaa ccg gta ttg act tac att aac caa        624
Ser Cys Glu Asp Leu Leu Pro Glu Pro Val Leu Thr Tyr Ile Asn Gln           
        195                 200                 205                       

caa ggc ttg tat cgc tga                                                642
Gln Gly Leu Tyr Arg                                                       
    210                                                                   


<210>  40
<211>  213
<212>  PRT
<213>  E. coli

<400>  40

Met Lys Ser Leu Gln Ala Leu Phe Gly Gly Thr Phe Asp Pro Val His 
1               5                   10                  15      


Tyr Gly His Leu Lys Pro Val Glu Thr Leu Ala Asn Leu Ile Gly Leu 
            20                  25                  30          


Thr Arg Val Thr Ile Ile Pro Asn Asn Val Pro Pro His Arg Pro Gln 
        35                  40                  45              


Pro Glu Ala Asn Ser Val Gln Arg Lys His Met Leu Glu Leu Ala Ile 
    50                  55                  60                  


Ala Asp Lys Pro Leu Phe Thr Leu Asp Glu Arg Glu Leu Lys Arg Asn 
65                  70                  75                  80  


Ala Pro Ser Tyr Thr Ala Gln Thr Leu Lys Glu Trp Arg Gln Glu Gln 
                85                  90                  95      


Gly Pro Asp Val Pro Leu Ala Phe Ile Ile Gly Gln Asp Ser Leu Leu 
            100                 105                 110         


Thr Phe Pro Thr Trp Tyr Glu Tyr Glu Thr Ile Leu Asp Asn Ala His 
        115                 120                 125             


Leu Ile Val Cys Arg Arg Pro Gly Tyr Pro Leu Glu Met Ala Gln Pro 
    130                 135                 140                 


Gln Tyr Gln Gln Trp Leu Glu Asp His Leu Thr His Asn Pro Glu Asp 
145                 150                 155                 160 


Leu His Leu Gln Pro Ala Gly Lys Ile Tyr Leu Ala Glu Thr Pro Trp 
                165                 170                 175     


Phe Asn Ile Ser Ala Thr Ile Ile Arg Glu Arg Leu Gln Asn Gly Glu 
            180                 185                 190         


Ser Cys Glu Asp Leu Leu Pro Glu Pro Val Leu Thr Tyr Ile Asn Gln 
        195                 200                 205             


Gln Gly Leu Tyr Arg 
    210             


<210>  41
<211>  333
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(333)
<223>  E. coli essential gene rplV

<400>  41
atg gaa act atc gct aaa cat cgc cat gct cgt tct tct gct cag aag         48
Met Glu Thr Ile Ala Lys His Arg His Ala Arg Ser Ser Ala Gln Lys           
1               5                   10                  15                

gtt cgc ctt gtt gct gac ctg att cgc ggt aag aaa gtg tcg cag gct         96
Val Arg Leu Val Ala Asp Leu Ile Arg Gly Lys Lys Val Ser Gln Ala           
            20                  25                  30                    

ctg gat att ttg acc tac acc aac aag aaa gcg gct gta ctg gtc aag        144
Leu Asp Ile Leu Thr Tyr Thr Asn Lys Lys Ala Ala Val Leu Val Lys           
        35                  40                  45                        

aaa gtt ctg gaa tct gcc att gct aac gct gaa cac aac gat ggc gct        192
Lys Val Leu Glu Ser Ala Ile Ala Asn Ala Glu His Asn Asp Gly Ala           
    50                  55                  60                            

gac att gac gat ctg aaa gtt acg aaa att ttc gta gac gaa ggc ccg        240
Asp Ile Asp Asp Leu Lys Val Thr Lys Ile Phe Val Asp Glu Gly Pro           
65                  70                  75                  80            

agc atg aag cgc att atg ccg cgt gca aaa ggt cgt gca gat cgc atc        288
Ser Met Lys Arg Ile Met Pro Arg Ala Lys Gly Arg Ala Asp Arg Ile           
                85                  90                  95                

ctg aag cgc acc agc cac atc act gtg gtt gtg tcc gat cgc tga            333
Leu Lys Arg Thr Ser His Ile Thr Val Val Val Ser Asp Arg                   
            100                 105                 110                   


<210>  42
<211>  110
<212>  PRT
<213>  E. coli

<400>  42

Met Glu Thr Ile Ala Lys His Arg His Ala Arg Ser Ser Ala Gln Lys 
1               5                   10                  15      


Val Arg Leu Val Ala Asp Leu Ile Arg Gly Lys Lys Val Ser Gln Ala 
            20                  25                  30          


Leu Asp Ile Leu Thr Tyr Thr Asn Lys Lys Ala Ala Val Leu Val Lys 
        35                  40                  45              


Lys Val Leu Glu Ser Ala Ile Ala Asn Ala Glu His Asn Asp Gly Ala 
    50                  55                  60                  


Asp Ile Asp Asp Leu Lys Val Thr Lys Ile Phe Val Asp Glu Gly Pro 
65                  70                  75                  80  


Ser Met Lys Arg Ile Met Pro Arg Ala Lys Gly Arg Ala Asp Arg Ile 
                85                  90                  95      


Leu Lys Arg Thr Ser His Ile Thr Val Val Val Ser Asp Arg 
            100                 105                 110 


<210>  43
<211>  471
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(471)
<223>  E. coli essential gene rpsG

<400>  43
atg cca cgt cgt cgc gtc att ggt cag cgt aaa att ctg ccg gat ccg         48
Met Pro Arg Arg Arg Val Ile Gly Gln Arg Lys Ile Leu Pro Asp Pro           
1               5                   10                  15                

aag ttc gga tca gaa ctg ctg gct aaa ttt gta aat atc ctg atg gta         96
Lys Phe Gly Ser Glu Leu Leu Ala Lys Phe Val Asn Ile Leu Met Val           
            20                  25                  30                    

gat ggt aaa aaa tct act gct gaa tct atc gta tac agc gcg ctg gag        144
Asp Gly Lys Lys Ser Thr Ala Glu Ser Ile Val Tyr Ser Ala Leu Glu           
        35                  40                  45                        

acc ctg gct cag cgc tct ggt aaa tct gaa ctg gaa gca ttc gaa gta        192
Thr Leu Ala Gln Arg Ser Gly Lys Ser Glu Leu Glu Ala Phe Glu Val           
    50                  55                  60                            

gct ctc gaa aac gtg cgc ccg act gta gaa gtt aag tct cgc cgc gtt        240
Ala Leu Glu Asn Val Arg Pro Thr Val Glu Val Lys Ser Arg Arg Val           
65                  70                  75                  80            

ggt ggt tct act tat cag gta cca gtt gaa gtc cgt ccg gtt cgt cgt        288
Gly Gly Ser Thr Tyr Gln Val Pro Val Glu Val Arg Pro Val Arg Arg           
                85                  90                  95                

aat gct ctg gca atg cgt tgg atc gtt gaa gct gct cgt aaa cgc ggt        336
Asn Ala Leu Ala Met Arg Trp Ile Val Glu Ala Ala Arg Lys Arg Gly           
            100                 105                 110                   

gat aaa tcc atg gct ctg cgc ctg gcg aac gaa ctt tct gat gct gca        384
Asp Lys Ser Met Ala Leu Arg Leu Ala Asn Glu Leu Ser Asp Ala Ala           
        115                 120                 125                       

gaa aac aaa ggt act gca gtt aag aaa cgt gaa gac gtt cac cgt atg        432
Glu Asn Lys Gly Thr Ala Val Lys Lys Arg Glu Asp Val His Arg Met           
    130                 135                 140                           

gcc gaa gcc aac aag gcg ttc gca cac tac cgt tgg taa                    471
Ala Glu Ala Asn Lys Ala Phe Ala His Tyr Arg Trp                           
145                 150                 155                               


<210>  44
<211>  156
<212>  PRT
<213>  E. coli

<400>  44

Met Pro Arg Arg Arg Val Ile Gly Gln Arg Lys Ile Leu Pro Asp Pro 
1               5                   10                  15      


Lys Phe Gly Ser Glu Leu Leu Ala Lys Phe Val Asn Ile Leu Met Val 
            20                  25                  30          


Asp Gly Lys Lys Ser Thr Ala Glu Ser Ile Val Tyr Ser Ala Leu Glu 
        35                  40                  45              


Thr Leu Ala Gln Arg Ser Gly Lys Ser Glu Leu Glu Ala Phe Glu Val 
    50                  55                  60                  


Ala Leu Glu Asn Val Arg Pro Thr Val Glu Val Lys Ser Arg Arg Val 
65                  70                  75                  80  


Gly Gly Ser Thr Tyr Gln Val Pro Val Glu Val Arg Pro Val Arg Arg 
                85                  90                  95      


Asn Ala Leu Ala Met Arg Trp Ile Val Glu Ala Ala Arg Lys Arg Gly 
            100                 105                 110         


Asp Lys Ser Met Ala Leu Arg Leu Ala Asn Glu Leu Ser Asp Ala Ala 
        115                 120                 125             


Glu Asn Lys Gly Thr Ala Val Lys Lys Arg Glu Asp Val His Arg Met 
    130                 135                 140                 


Ala Glu Ala Asn Lys Ala Phe Ala His Tyr Arg Trp 
145                 150                 155     


<210>  45
<211>  2475
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(2475)
<223>  S. cerevisiae essential gene FOL1 YNL256W SGDID:S000005200

<400>  45
atg tca aag cta ttt tct act gtc aat tct gca aga cat agt gta cca         48
Met Ser Lys Leu Phe Ser Thr Val Asn Ser Ala Arg His Ser Val Pro           
1               5                   10                  15                

cta ggc ggc atg aga gat tat gtg cac att aag aaa cta gag atg aat         96
Leu Gly Gly Met Arg Asp Tyr Val His Ile Lys Lys Leu Glu Met Asn           
            20                  25                  30                    

aca gtt ctt ggg cct gat tcc tgg aat caa tta atg cct cag aaa tgt        144
Thr Val Leu Gly Pro Asp Ser Trp Asn Gln Leu Met Pro Gln Lys Cys           
        35                  40                  45                        

cta cta agc tta gat atg ggt aca gat ttt agt aaa tct gcg gct acg        192
Leu Leu Ser Leu Asp Met Gly Thr Asp Phe Ser Lys Ser Ala Ala Thr           
    50                  55                  60                            

gat gat ttg aaa tat tct cta aat tat gca gtt att tct cgt gat ttg        240
Asp Asp Leu Lys Tyr Ser Leu Asn Tyr Ala Val Ile Ser Arg Asp Leu           
65                  70                  75                  80            

acg aat ttc gtc agc aaa aaa aag aat tgg ggt tct gtt tct aat ttg        288
Thr Asn Phe Val Ser Lys Lys Lys Asn Trp Gly Ser Val Ser Asn Leu           
                85                  90                  95                

gct aaa tct gtg tct caa ttt gtt atg gac aaa tat tct ggt gtc gag        336
Ala Lys Ser Val Ser Gln Phe Val Met Asp Lys Tyr Ser Gly Val Glu           
            100                 105                 110                   

tgt ctg aat tta gaa gtg cag gcg gat aca acg cat att aga agt gac        384
Cys Leu Asn Leu Glu Val Gln Ala Asp Thr Thr His Ile Arg Ser Asp           
        115                 120                 125                       

cac ata tct tgt att att caa caa gaa aga ggg aat cca gaa tca cag        432
His Ile Ser Cys Ile Ile Gln Gln Glu Arg Gly Asn Pro Glu Ser Gln           
    130                 135                 140                           

gaa ttt gac gtt gtt agg ata tct gag tta aaa atg ttg act ttg att        480
Glu Phe Asp Val Val Arg Ile Ser Glu Leu Lys Met Leu Thr Leu Ile           
145                 150                 155                 160           

ggt gtt ttc acc ttt gag aga ctt aag aaa cag tat gta act ttg gat        528
Gly Val Phe Thr Phe Glu Arg Leu Lys Lys Gln Tyr Val Thr Leu Asp           
                165                 170                 175               

ata aag ttg cct tgg cca aag aaa gcc gaa ttg cca ccg cca gtg caa        576
Ile Lys Leu Pro Trp Pro Lys Lys Ala Glu Leu Pro Pro Pro Val Gln           
            180                 185                 190                   

agc ata att gat aac gtt gtc aag ttt gtg gag gaa tca aat ttc aag        624
Ser Ile Ile Asp Asn Val Val Lys Phe Val Glu Glu Ser Asn Phe Lys           
        195                 200                 205                       

act gtg gaa gct ctt gta gaa tct gtg tca gct gtt att gcc cat aac        672
Thr Val Glu Ala Leu Val Glu Ser Val Ser Ala Val Ile Ala His Asn           
    210                 215                 220                           

gag tat ttt caa aag ttt cca gat tcg cct ttg gtg gtg aag gtt ttg        720
Glu Tyr Phe Gln Lys Phe Pro Asp Ser Pro Leu Val Val Lys Val Leu           
225                 230                 235                 240           

aaa tta aac gca atc aca gcc aca gaa ggt gtt ggt gta agc tgt att        768
Lys Leu Asn Ala Ile Thr Ala Thr Glu Gly Val Gly Val Ser Cys Ile           
                245                 250                 255               

aga gag ccc agg gag att gcg atg gta aat att cca tat ctt tcc tcc        816
Arg Glu Pro Arg Glu Ile Ala Met Val Asn Ile Pro Tyr Leu Ser Ser           
            260                 265                 270                   

ata cat gaa tcg tct gat att aag ttc caa ttg tct tca tca caa aac        864
Ile His Glu Ser Ser Asp Ile Lys Phe Gln Leu Ser Ser Ser Gln Asn           
        275                 280                 285                       

act cct att gag ggt aaa aat aca tgg aaa aga gcg ttt tta gcg ttt        912
Thr Pro Ile Glu Gly Lys Asn Thr Trp Lys Arg Ala Phe Leu Ala Phe           
    290                 295                 300                           

ggt tca aac att ggg gac cgt ttc aaa cac att caa atg gcg ttg caa        960
Gly Ser Asn Ile Gly Asp Arg Phe Lys His Ile Gln Met Ala Leu Gln           
305                 310                 315                 320           

tta tta tca agg gaa aaa acg gtt aaa tta cgg aat att tcg tct att       1008
Leu Leu Ser Arg Glu Lys Thr Val Lys Leu Arg Asn Ile Ser Ser Ile           
                325                 330                 335               

ttt gaa agt gaa cca atg tat ttc aaa gat caa acc cct ttc atg aat       1056
Phe Glu Ser Glu Pro Met Tyr Phe Lys Asp Gln Thr Pro Phe Met Asn           
            340                 345                 350                   

ggg tgt gtt gag gtg gag aca tta ctg acc cca agc gaa tta tta aaa       1104
Gly Cys Val Glu Val Glu Thr Leu Leu Thr Pro Ser Glu Leu Leu Lys           
        355                 360                 365                       

ttg tgt aaa aaa att gaa tat gaa gag ttg caa aga gtc aag cat ttt       1152
Leu Cys Lys Lys Ile Glu Tyr Glu Glu Leu Gln Arg Val Lys His Phe           
    370                 375                 380                           

gat aat ggt ccg aga aca ata gat ctg gat att gtt atg ttt ttg aat       1200
Asp Asn Gly Pro Arg Thr Ile Asp Leu Asp Ile Val Met Phe Leu Asn           
385                 390                 395                 400           

agc gcc gga gaa gat att ata gta aat gaa ccg gat ttg aat ata ccg       1248
Ser Ala Gly Glu Asp Ile Ile Val Asn Glu Pro Asp Leu Asn Ile Pro           
                405                 410                 415               

cat cct aga atg ctg gag agg act ttc gtt ctt gag ccg tta tgt gaa       1296
His Pro Arg Met Leu Glu Arg Thr Phe Val Leu Glu Pro Leu Cys Glu           
            420                 425                 430                   

tta ata tcc ccc gtt cac ctt cat cct gtg aca gcg gaa ccc att gta       1344
Leu Ile Ser Pro Val His Leu His Pro Val Thr Ala Glu Pro Ile Val           
        435                 440                 445                       

gac cat tta aaa cag tta tac gac aaa cag cat gat gaa gat acc tta       1392
Asp His Leu Lys Gln Leu Tyr Asp Lys Gln His Asp Glu Asp Thr Leu           
    450                 455                 460                           

tgg aaa tta gtt cca ttg cct tat cgt agt ggt gtg gag cct aga ttt       1440
Trp Lys Leu Val Pro Leu Pro Tyr Arg Ser Gly Val Glu Pro Arg Phe           
465                 470                 475                 480           

ttg aaa ttc aag acc gct aca aaa ctt gac gaa ttt act gga gaa aca       1488
Leu Lys Phe Lys Thr Ala Thr Lys Leu Asp Glu Phe Thr Gly Glu Thr           
                485                 490                 495               

aac aga att act gtt tca cct aca tat atc atg gct atc ttc aac gct       1536
Asn Arg Ile Thr Val Ser Pro Thr Tyr Ile Met Ala Ile Phe Asn Ala           
            500                 505                 510                   

aca cca gat tca ttt tcc gat gga ggt gag cat ttt gcg gac att gaa       1584
Thr Pro Asp Ser Phe Ser Asp Gly Gly Glu His Phe Ala Asp Ile Glu           
        515                 520                 525                       

agt caa ttg aat gat atc att aaa ttg tgt aaa gac gca tta tat ttg       1632
Ser Gln Leu Asn Asp Ile Ile Lys Leu Cys Lys Asp Ala Leu Tyr Leu           
    530                 535                 540                           

cat gag agc gtc atc atc gac gtt gga ggg tgt tct acc agg cct aac       1680
His Glu Ser Val Ile Ile Asp Val Gly Gly Cys Ser Thr Arg Pro Asn           
545                 550                 555                 560           

tct att cag gcg tct gag gaa gaa gaa ata cgc agg tct atc cca tta       1728
Ser Ile Gln Ala Ser Glu Glu Glu Glu Ile Arg Arg Ser Ile Pro Leu           
                565                 570                 575               

att aag gcc att aga gaa agc act gag tta ccg caa gat aaa gtc ata       1776
Ile Lys Ala Ile Arg Glu Ser Thr Glu Leu Pro Gln Asp Lys Val Ile           
            580                 585                 590                   

cta tcc att gat act tat cgt tcc aat gtc gct aaa gaa gcg att aaa       1824
Leu Ser Ile Asp Thr Tyr Arg Ser Asn Val Ala Lys Glu Ala Ile Lys           
        595                 600                 605                       

gtt gga gtg gat att att aat gat att tcg gga ggt tta ttt gac agc       1872
Val Gly Val Asp Ile Ile Asn Asp Ile Ser Gly Gly Leu Phe Asp Ser           
    610                 615                 620                           

aac atg ttt gcc gta att gca gag aac cca gaa att tgt tat att tta       1920
Asn Met Phe Ala Val Ile Ala Glu Asn Pro Glu Ile Cys Tyr Ile Leu           
625                 630                 635                 640           

tca cac aca cgt ggt gat att tca acg atg aat agg ctg gcg cat tac       1968
Ser His Thr Arg Gly Asp Ile Ser Thr Met Asn Arg Leu Ala His Tyr           
                645                 650                 655               

gaa aat ttt gca ttg ggt gat tct att cag caa gaa ttt gtt cat aat       2016
Glu Asn Phe Ala Leu Gly Asp Ser Ile Gln Gln Glu Phe Val His Asn           
            660                 665                 670                   

acc gac att cag cag cta gac gac ttg aaa gac aaa aca gtg tta atc       2064
Thr Asp Ile Gln Gln Leu Asp Asp Leu Lys Asp Lys Thr Val Leu Ile           
        675                 680                 685                       

agg aat gtt ggt caa gaa att ggc gaa agg tat atc aaa gcg att gat       2112
Arg Asn Val Gly Gln Glu Ile Gly Glu Arg Tyr Ile Lys Ala Ile Asp           
    690                 695                 700                           

aat gga gta aag cgc tgg caa att cta atc gac cct gga ctt ggt ttt       2160
Asn Gly Val Lys Arg Trp Gln Ile Leu Ile Asp Pro Gly Leu Gly Phe           
705                 710                 715                 720           

gct aag acc tgg aag caa aac tta caa att att aga cat atc ccc att       2208
Ala Lys Thr Trp Lys Gln Asn Leu Gln Ile Ile Arg His Ile Pro Ile           
                725                 730                 735               

tta aag aac tac tca ttc acc atg aac tca aac aat tcg caa gtg tat       2256
Leu Lys Asn Tyr Ser Phe Thr Met Asn Ser Asn Asn Ser Gln Val Tyr           
            740                 745                 750                   

gtt aac ctc aga aat atg ccc gtt tta ttg ggt cca tcg cgc aaa aaa       2304
Val Asn Leu Arg Asn Met Pro Val Leu Leu Gly Pro Ser Arg Lys Lys           
        755                 760                 765                       

ttc att gga cat atc aca aaa gat gtg gat gcg aag caa aga gac ttt       2352
Phe Ile Gly His Ile Thr Lys Asp Val Asp Ala Lys Gln Arg Asp Phe           
    770                 775                 780                           

gct act gga gcg gtg gta gcg tcg tgt att ggt ttc ggc agc gac atg       2400
Ala Thr Gly Ala Val Val Ala Ser Cys Ile Gly Phe Gly Ser Asp Met           
785                 790                 795                 800           

gtt agg gtc cat gac gtt aaa aat tgt tcg aag agc att aaa tta gca       2448
Val Arg Val His Asp Val Lys Asn Cys Ser Lys Ser Ile Lys Leu Ala           
                805                 810                 815               

gat gct att tat aaa ggt ttg gaa taa                                   2475
Asp Ala Ile Tyr Lys Gly Leu Glu                                           
            820                                                           


<210>  46
<211>  824
<212>  PRT
<213>  S. cerevisiae

<400>  46

Met Ser Lys Leu Phe Ser Thr Val Asn Ser Ala Arg His Ser Val Pro 
1               5                   10                  15      


Leu Gly Gly Met Arg Asp Tyr Val His Ile Lys Lys Leu Glu Met Asn 
            20                  25                  30          


Thr Val Leu Gly Pro Asp Ser Trp Asn Gln Leu Met Pro Gln Lys Cys 
        35                  40                  45              


Leu Leu Ser Leu Asp Met Gly Thr Asp Phe Ser Lys Ser Ala Ala Thr 
    50                  55                  60                  


Asp Asp Leu Lys Tyr Ser Leu Asn Tyr Ala Val Ile Ser Arg Asp Leu 
65                  70                  75                  80  


Thr Asn Phe Val Ser Lys Lys Lys Asn Trp Gly Ser Val Ser Asn Leu 
                85                  90                  95      


Ala Lys Ser Val Ser Gln Phe Val Met Asp Lys Tyr Ser Gly Val Glu 
            100                 105                 110         


Cys Leu Asn Leu Glu Val Gln Ala Asp Thr Thr His Ile Arg Ser Asp 
        115                 120                 125             


His Ile Ser Cys Ile Ile Gln Gln Glu Arg Gly Asn Pro Glu Ser Gln 
    130                 135                 140                 


Glu Phe Asp Val Val Arg Ile Ser Glu Leu Lys Met Leu Thr Leu Ile 
145                 150                 155                 160 


Gly Val Phe Thr Phe Glu Arg Leu Lys Lys Gln Tyr Val Thr Leu Asp 
                165                 170                 175     


Ile Lys Leu Pro Trp Pro Lys Lys Ala Glu Leu Pro Pro Pro Val Gln 
            180                 185                 190         


Ser Ile Ile Asp Asn Val Val Lys Phe Val Glu Glu Ser Asn Phe Lys 
        195                 200                 205             


Thr Val Glu Ala Leu Val Glu Ser Val Ser Ala Val Ile Ala His Asn 
    210                 215                 220                 


Glu Tyr Phe Gln Lys Phe Pro Asp Ser Pro Leu Val Val Lys Val Leu 
225                 230                 235                 240 


Lys Leu Asn Ala Ile Thr Ala Thr Glu Gly Val Gly Val Ser Cys Ile 
                245                 250                 255     


Arg Glu Pro Arg Glu Ile Ala Met Val Asn Ile Pro Tyr Leu Ser Ser 
            260                 265                 270         


Ile His Glu Ser Ser Asp Ile Lys Phe Gln Leu Ser Ser Ser Gln Asn 
        275                 280                 285             


Thr Pro Ile Glu Gly Lys Asn Thr Trp Lys Arg Ala Phe Leu Ala Phe 
    290                 295                 300                 


Gly Ser Asn Ile Gly Asp Arg Phe Lys His Ile Gln Met Ala Leu Gln 
305                 310                 315                 320 


Leu Leu Ser Arg Glu Lys Thr Val Lys Leu Arg Asn Ile Ser Ser Ile 
                325                 330                 335     


Phe Glu Ser Glu Pro Met Tyr Phe Lys Asp Gln Thr Pro Phe Met Asn 
            340                 345                 350         


Gly Cys Val Glu Val Glu Thr Leu Leu Thr Pro Ser Glu Leu Leu Lys 
        355                 360                 365             


Leu Cys Lys Lys Ile Glu Tyr Glu Glu Leu Gln Arg Val Lys His Phe 
    370                 375                 380                 


Asp Asn Gly Pro Arg Thr Ile Asp Leu Asp Ile Val Met Phe Leu Asn 
385                 390                 395                 400 


Ser Ala Gly Glu Asp Ile Ile Val Asn Glu Pro Asp Leu Asn Ile Pro 
                405                 410                 415     


His Pro Arg Met Leu Glu Arg Thr Phe Val Leu Glu Pro Leu Cys Glu 
            420                 425                 430         


Leu Ile Ser Pro Val His Leu His Pro Val Thr Ala Glu Pro Ile Val 
        435                 440                 445             


Asp His Leu Lys Gln Leu Tyr Asp Lys Gln His Asp Glu Asp Thr Leu 
    450                 455                 460                 


Trp Lys Leu Val Pro Leu Pro Tyr Arg Ser Gly Val Glu Pro Arg Phe 
465                 470                 475                 480 


Leu Lys Phe Lys Thr Ala Thr Lys Leu Asp Glu Phe Thr Gly Glu Thr 
                485                 490                 495     


Asn Arg Ile Thr Val Ser Pro Thr Tyr Ile Met Ala Ile Phe Asn Ala 
            500                 505                 510         


Thr Pro Asp Ser Phe Ser Asp Gly Gly Glu His Phe Ala Asp Ile Glu 
        515                 520                 525             


Ser Gln Leu Asn Asp Ile Ile Lys Leu Cys Lys Asp Ala Leu Tyr Leu 
    530                 535                 540                 


His Glu Ser Val Ile Ile Asp Val Gly Gly Cys Ser Thr Arg Pro Asn 
545                 550                 555                 560 


Ser Ile Gln Ala Ser Glu Glu Glu Glu Ile Arg Arg Ser Ile Pro Leu 
                565                 570                 575     


Ile Lys Ala Ile Arg Glu Ser Thr Glu Leu Pro Gln Asp Lys Val Ile 
            580                 585                 590         


Leu Ser Ile Asp Thr Tyr Arg Ser Asn Val Ala Lys Glu Ala Ile Lys 
        595                 600                 605             


Val Gly Val Asp Ile Ile Asn Asp Ile Ser Gly Gly Leu Phe Asp Ser 
    610                 615                 620                 


Asn Met Phe Ala Val Ile Ala Glu Asn Pro Glu Ile Cys Tyr Ile Leu 
625                 630                 635                 640 


Ser His Thr Arg Gly Asp Ile Ser Thr Met Asn Arg Leu Ala His Tyr 
                645                 650                 655     


Glu Asn Phe Ala Leu Gly Asp Ser Ile Gln Gln Glu Phe Val His Asn 
            660                 665                 670         


Thr Asp Ile Gln Gln Leu Asp Asp Leu Lys Asp Lys Thr Val Leu Ile 
        675                 680                 685             


Arg Asn Val Gly Gln Glu Ile Gly Glu Arg Tyr Ile Lys Ala Ile Asp 
    690                 695                 700                 


Asn Gly Val Lys Arg Trp Gln Ile Leu Ile Asp Pro Gly Leu Gly Phe 
705                 710                 715                 720 


Ala Lys Thr Trp Lys Gln Asn Leu Gln Ile Ile Arg His Ile Pro Ile 
                725                 730                 735     


Leu Lys Asn Tyr Ser Phe Thr Met Asn Ser Asn Asn Ser Gln Val Tyr 
            740                 745                 750         


Val Asn Leu Arg Asn Met Pro Val Leu Leu Gly Pro Ser Arg Lys Lys 
        755                 760                 765             


Phe Ile Gly His Ile Thr Lys Asp Val Asp Ala Lys Gln Arg Asp Phe 
    770                 775                 780                 


Ala Thr Gly Ala Val Val Ala Ser Cys Ile Gly Phe Gly Ser Asp Met 
785                 790                 795                 800 


Val Arg Val His Asp Val Lys Asn Cys Ser Lys Ser Ile Lys Leu Ala 
                805                 810                 815     


Asp Ala Ile Tyr Lys Gly Leu Glu 
            820                 


<210>  47
<211>  669
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(669)
<223>  S. cerevisiae essential gene MED7 YOL135C SGDID:S000005495

<400>  47
atg tcc aat gat cct ggt aat gag gtt agc tcc ttg tac cca cct cca         48
Met Ser Asn Asp Pro Gly Asn Glu Val Ser Ser Leu Tyr Pro Pro Pro           
1               5                   10                  15                

ccc cca tac gtg aag ttt ttc aca cag tcc aat ctg gaa aaa tta cca         96
Pro Pro Tyr Val Lys Phe Phe Thr Gln Ser Asn Leu Glu Lys Leu Pro           
            20                  25                  30                    

aaa tac aaa gaa aag aag gcc gca agt gcg aag cag acg gct cca aat        144
Lys Tyr Lys Glu Lys Lys Ala Ala Ser Ala Lys Gln Thr Ala Pro Asn           
        35                  40                  45                        

aac agt aac ggc ggc agt gaa gag gag att aca tgt gcg cta gat tac        192
Asn Ser Asn Gly Gly Ser Glu Glu Glu Ile Thr Cys Ala Leu Asp Tyr           
    50                  55                  60                            

cta atc cca ccc ccc atg ccc aaa aat cag cag tac cga gcg ttt ggg        240
Leu Ile Pro Pro Pro Met Pro Lys Asn Gln Gln Tyr Arg Ala Phe Gly           
65                  70                  75                  80            

agt ata tgg cag gtg aag gac caa ctg ccc gat tta gag agc atg gga        288
Ser Ile Trp Gln Val Lys Asp Gln Leu Pro Asp Leu Glu Ser Met Gly           
                85                  90                  95                

ctc acc caa ctt tat aag aaa tcc aca gaa aac gaa agc aca aat tat        336
Leu Thr Gln Leu Tyr Lys Lys Ser Thr Glu Asn Glu Ser Thr Asn Tyr           
            100                 105                 110                   

caa tac aaa atc caa gag cta cgc aaa cta ttg aaa tcc ctc ctg cta        384
Gln Tyr Lys Ile Gln Glu Leu Arg Lys Leu Leu Lys Ser Leu Leu Leu           
        115                 120                 125                       

aat tac ttg gaa ctc atc ggc gta ttg agc ata aat ccg gat atg tat        432
Asn Tyr Leu Glu Leu Ile Gly Val Leu Ser Ile Asn Pro Asp Met Tyr           
    130                 135                 140                           

gag cgc aaa gtg gag aac ata agg acc att ctt gtc aat att cac cac        480
Glu Arg Lys Val Glu Asn Ile Arg Thr Ile Leu Val Asn Ile His His           
145                 150                 155                 160           

cta ctt aat gag tac aga ccc cat cag tcc cga gag tcc ctt att atg        528
Leu Leu Asn Glu Tyr Arg Pro His Gln Ser Arg Glu Ser Leu Ile Met           
                165                 170                 175               

cta ttg gaa gaa caa tta gaa tac aag cgc ggg gaa att cgc gag atc        576
Leu Leu Glu Glu Gln Leu Glu Tyr Lys Arg Gly Glu Ile Arg Glu Ile           
            180                 185                 190                   

gag caa gta tgc aaa cag gta cac gac aaa ctc acc agc ata cag gac        624
Glu Gln Val Cys Lys Gln Val His Asp Lys Leu Thr Ser Ile Gln Asp           
        195                 200                 205                       

acc tta aga acc gga tca caa tct cct cca tcg tcg tcc caa tga            669
Thr Leu Arg Thr Gly Ser Gln Ser Pro Pro Ser Ser Ser Gln                   
    210                 215                 220                           


<210>  48
<211>  222
<212>  PRT
<213>  S. cerevisiae

<400>  48

Met Ser Asn Asp Pro Gly Asn Glu Val Ser Ser Leu Tyr Pro Pro Pro 
1               5                   10                  15      


Pro Pro Tyr Val Lys Phe Phe Thr Gln Ser Asn Leu Glu Lys Leu Pro 
            20                  25                  30          


Lys Tyr Lys Glu Lys Lys Ala Ala Ser Ala Lys Gln Thr Ala Pro Asn 
        35                  40                  45              


Asn Ser Asn Gly Gly Ser Glu Glu Glu Ile Thr Cys Ala Leu Asp Tyr 
    50                  55                  60                  


Leu Ile Pro Pro Pro Met Pro Lys Asn Gln Gln Tyr Arg Ala Phe Gly 
65                  70                  75                  80  


Ser Ile Trp Gln Val Lys Asp Gln Leu Pro Asp Leu Glu Ser Met Gly 
                85                  90                  95      


Leu Thr Gln Leu Tyr Lys Lys Ser Thr Glu Asn Glu Ser Thr Asn Tyr 
            100                 105                 110         


Gln Tyr Lys Ile Gln Glu Leu Arg Lys Leu Leu Lys Ser Leu Leu Leu 
        115                 120                 125             


Asn Tyr Leu Glu Leu Ile Gly Val Leu Ser Ile Asn Pro Asp Met Tyr 
    130                 135                 140                 


Glu Arg Lys Val Glu Asn Ile Arg Thr Ile Leu Val Asn Ile His His 
145                 150                 155                 160 


Leu Leu Asn Glu Tyr Arg Pro His Gln Ser Arg Glu Ser Leu Ile Met 
                165                 170                 175     


Leu Leu Glu Glu Gln Leu Glu Tyr Lys Arg Gly Glu Ile Arg Glu Ile 
            180                 185                 190         


Glu Gln Val Cys Lys Gln Val His Asp Lys Leu Thr Ser Ile Gln Asp 
        195                 200                 205             


Thr Leu Arg Thr Gly Ser Gln Ser Pro Pro Ser Ser Ser Gln 
    210                 215                 220         


<210>  49
<211>  723
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(723)
<223>  S. cerevisiae essential gene RRP40 YOL142W SGDID:S000005502

<400>  49
atg tct acg ttc ata ttc cct ggt gat agc ttt cct gta gac cct act         48
Met Ser Thr Phe Ile Phe Pro Gly Asp Ser Phe Pro Val Asp Pro Thr           
1               5                   10                  15                

aca cct gtt aaa ctg ggc cct ggc ata tat tgt gac ccc aat act caa         96
Thr Pro Val Lys Leu Gly Pro Gly Ile Tyr Cys Asp Pro Asn Thr Gln           
            20                  25                  30                    

gaa ata cga cct gtt aat aca ggt gtt ttg cat gtt tcc gct aag ggt        144
Glu Ile Arg Pro Val Asn Thr Gly Val Leu His Val Ser Ala Lys Gly           
        35                  40                  45                        

aag agt ggt gtt cag acc gca tat ata gac tat tct agt aag aga tac        192
Lys Ser Gly Val Gln Thr Ala Tyr Ile Asp Tyr Ser Ser Lys Arg Tyr           
    50                  55                  60                            

att cca tct gta aac gat ttt gta atc ggt gtc att ata ggg aca ttt        240
Ile Pro Ser Val Asn Asp Phe Val Ile Gly Val Ile Ile Gly Thr Phe           
65                  70                  75                  80            

tca gat agc tat aag gtt tcg ttg caa aat ttc tcc tcc agt gtt tca        288
Ser Asp Ser Tyr Lys Val Ser Leu Gln Asn Phe Ser Ser Ser Val Ser           
                85                  90                  95                

cta tcg tat atg gct ttt ccc aat gct tca aag aaa aac agg cca act        336
Leu Ser Tyr Met Ala Phe Pro Asn Ala Ser Lys Lys Asn Arg Pro Thr           
            100                 105                 110                   

ttg caa gtg gga gat cta gtg tat gcg aga gtt tgt acc gca gaa aag        384
Leu Gln Val Gly Asp Leu Val Tyr Ala Arg Val Cys Thr Ala Glu Lys           
        115                 120                 125                       

gaa cta gaa gcc gaa ata gaa tgt ttt gac tca act aca gga cgc gat        432
Glu Leu Glu Ala Glu Ile Glu Cys Phe Asp Ser Thr Thr Gly Arg Asp           
    130                 135                 140                           

gct ggt ttc ggg ata ttg gaa gat ggt atg atc att gac gtg aat ttg        480
Ala Gly Phe Gly Ile Leu Glu Asp Gly Met Ile Ile Asp Val Asn Leu           
145                 150                 155                 160           

aat ttc gca cgc cag ttg ctt ttc aat aat gac ttc ccg tta tta aaa        528
Asn Phe Ala Arg Gln Leu Leu Phe Asn Asn Asp Phe Pro Leu Leu Lys           
                165                 170                 175               

gtg ttg gct gca cat acc aag ttt gaa gtc gcc att ggt ctc aat ggg        576
Val Leu Ala Ala His Thr Lys Phe Glu Val Ala Ile Gly Leu Asn Gly           
            180                 185                 190                   

aag atc tgg gtt aag tgc gag gaa tta tct aac act tta gct tgt tat        624
Lys Ile Trp Val Lys Cys Glu Glu Leu Ser Asn Thr Leu Ala Cys Tyr           
        195                 200                 205                       

aga acc ata atg gag tgt tgt caa aaa aac gac acg gca gcg ttc aag        672
Arg Thr Ile Met Glu Cys Cys Gln Lys Asn Asp Thr Ala Ala Phe Lys           
    210                 215                 220                           

gat ata gca aaa aga cag ttt aaa gaa ata ctt acg gtc aag gag gag        720
Asp Ile Ala Lys Arg Gln Phe Lys Glu Ile Leu Thr Val Lys Glu Glu           
225                 230                 235                 240           

tag                                                                    723


<210>  50
<211>  240
<212>  PRT
<213>  S. cerevisiae

<400>  50

Met Ser Thr Phe Ile Phe Pro Gly Asp Ser Phe Pro Val Asp Pro Thr 
1               5                   10                  15      


Thr Pro Val Lys Leu Gly Pro Gly Ile Tyr Cys Asp Pro Asn Thr Gln 
            20                  25                  30          


Glu Ile Arg Pro Val Asn Thr Gly Val Leu His Val Ser Ala Lys Gly 
        35                  40                  45              


Lys Ser Gly Val Gln Thr Ala Tyr Ile Asp Tyr Ser Ser Lys Arg Tyr 
    50                  55                  60                  


Ile Pro Ser Val Asn Asp Phe Val Ile Gly Val Ile Ile Gly Thr Phe 
65                  70                  75                  80  


Ser Asp Ser Tyr Lys Val Ser Leu Gln Asn Phe Ser Ser Ser Val Ser 
                85                  90                  95      


Leu Ser Tyr Met Ala Phe Pro Asn Ala Ser Lys Lys Asn Arg Pro Thr 
            100                 105                 110         


Leu Gln Val Gly Asp Leu Val Tyr Ala Arg Val Cys Thr Ala Glu Lys 
        115                 120                 125             


Glu Leu Glu Ala Glu Ile Glu Cys Phe Asp Ser Thr Thr Gly Arg Asp 
    130                 135                 140                 


Ala Gly Phe Gly Ile Leu Glu Asp Gly Met Ile Ile Asp Val Asn Leu 
145                 150                 155                 160 


Asn Phe Ala Arg Gln Leu Leu Phe Asn Asn Asp Phe Pro Leu Leu Lys 
                165                 170                 175     


Val Leu Ala Ala His Thr Lys Phe Glu Val Ala Ile Gly Leu Asn Gly 
            180                 185                 190         


Lys Ile Trp Val Lys Cys Glu Glu Leu Ser Asn Thr Leu Ala Cys Tyr 
        195                 200                 205             


Arg Thr Ile Met Glu Cys Cys Gln Lys Asn Asp Thr Ala Ala Phe Lys 
    210                 215                 220                 


Asp Ile Ala Lys Arg Gln Phe Lys Glu Ile Leu Thr Val Lys Glu Glu 
225                 230                 235                 240 


<210>  51
<211>  1455
<212>  DNA
<213>  S. cerevisiea


<220>
<221>  CDS
<222>  (1)..(1455)
<223>  S. cerevisiae essential gene NOP8 YOL144W SGDID:S000005504

<400>  51
atg gat agt gta att caa aaa aga att ttt gtc gga aat att ttc cat         48
Met Asp Ser Val Ile Gln Lys Arg Ile Phe Val Gly Asn Ile Phe His           
1               5                   10                  15                

aat gca gat gat tgt tat tca gaa cta cta gat cga ttt gga aaa ttt         96
Asn Ala Asp Asp Cys Tyr Ser Glu Leu Leu Asp Arg Phe Gly Lys Phe           
            20                  25                  30                    

ggt gat tgc caa gat ttt cag ttt gaa aaa cac aac cac ttt gca ttt        144
Gly Asp Cys Gln Asp Phe Gln Phe Glu Lys His Asn His Phe Ala Phe           
        35                  40                  45                        

atc gat ata agg ttt aat gat gag gca gat ttt aac aag cta aga aag        192
Ile Asp Ile Arg Phe Asn Asp Glu Ala Asp Phe Asn Lys Leu Arg Lys           
    50                  55                  60                            

agc ttt aat aat gtt aag ttt aaa ggg aac att ttg aag gtt gat gaa        240
Ser Phe Asn Asn Val Lys Phe Lys Gly Asn Ile Leu Lys Val Asp Glu           
65                  70                  75                  80            

gca aag ccg aat tgg gaa agc acc tgg gcg gta caa cat gca aag gac        288
Ala Lys Pro Asn Trp Glu Ser Thr Trp Ala Val Gln His Ala Lys Asp           
                85                  90                  95                

ctt aaa gaa gat att ata cta aat gct aaa atg aaa aaa aag aat tgg        336
Leu Lys Glu Asp Ile Ile Leu Asn Ala Lys Met Lys Lys Lys Asn Trp           
            100                 105                 110                   

caa cat tat aag aaa atg gaa aat gta gcg aaa agt tgg aaa gat cat        384
Gln His Tyr Lys Lys Met Glu Asn Val Ala Lys Ser Trp Lys Asp His           
        115                 120                 125                       

aag gaa gtt att gct ggc aga atg agg gag gcg ccg aga aaa agg agt        432
Lys Glu Val Ile Ala Gly Arg Met Arg Glu Ala Pro Arg Lys Arg Ser           
    130                 135                 140                           

caa ttg agg aac att aca ttc agg atc aat gtc aat ggt tca ttg aaa        480
Gln Leu Arg Asn Ile Thr Phe Arg Ile Asn Val Asn Gly Ser Leu Lys           
145                 150                 155                 160           

gtt tac aaa tgc tat aag act aaa tta tgg ggt tat gaa agg aac aaa        528
Val Tyr Lys Cys Tyr Lys Thr Lys Leu Trp Gly Tyr Glu Arg Asn Lys           
                165                 170                 175               

gaa ttg aat gac ctt gtg tac aag ttt aca aat aat ttc tgg aaa aac        576
Glu Leu Asn Asp Leu Val Tyr Lys Phe Thr Asn Asn Phe Trp Lys Asn           
            180                 185                 190                   

gga tac aac cac att gtc gat aga ttg gat tac agt cgt gct gtt aaa        624
Gly Tyr Asn His Ile Val Asp Arg Leu Asp Tyr Ser Arg Ala Val Lys           
        195                 200                 205                       

act gtt cga ttc aaa aat ggg ctg aaa cag tta aca gtg tca aaa gat        672
Thr Val Arg Phe Lys Asn Gly Leu Lys Gln Leu Thr Val Ser Lys Asp           
    210                 215                 220                           

gaa aac gtt tgc agt gga gag atg gac agc gat gag aat atg tca gaa        720
Glu Asn Val Cys Ser Gly Glu Met Asp Ser Asp Glu Asn Met Ser Glu           
225                 230                 235                 240           

gag gag aag gag aaa aat aat gtt att ctt aat gac cta tta aag gac        768
Glu Glu Lys Glu Lys Asn Asn Val Ile Leu Asn Asp Leu Leu Lys Asp           
                245                 250                 255               

ttt gac ttc gat aag cca atg acg ttg aat gat tcg gac gag gaa ctt        816
Phe Asp Phe Asp Lys Pro Met Thr Leu Asn Asp Ser Asp Glu Glu Leu           
            260                 265                 270                   

ctg aca gag caa cgt aaa gga gag gaa gaa gaa gaa gaa gaa gaa gaa        864
Leu Thr Glu Gln Arg Lys Gly Glu Glu Glu Glu Glu Glu Glu Glu Glu           
        275                 280                 285                       

aaa gaa gta aac gct ccc gaa tac gag aat gtt aat aag acg aag gac        912
Lys Glu Val Asn Ala Pro Glu Tyr Glu Asn Val Asn Lys Thr Lys Asp           
    290                 295                 300                           

cag agc acc cta cca caa gaa aaa cca gag gaa agg aaa gaa cag gac        960
Gln Ser Thr Leu Pro Gln Glu Lys Pro Glu Glu Arg Lys Glu Gln Asp           
305                 310                 315                 320           

gag ggc gac ggg cag gag gat aat gaa ttt att ccg act ttc acc aag       1008
Glu Gly Asp Gly Gln Glu Asp Asn Glu Phe Ile Pro Thr Phe Thr Lys           
                325                 330                 335               

gaa att ggt caa ggt aca ata agc aac act gag acg tta aga aac cta       1056
Glu Ile Gly Gln Gly Thr Ile Ser Asn Thr Glu Thr Leu Arg Asn Leu           
            340                 345                 350                   

ttc aac ccc aac gag gca gaa cct gta tca caa ttc aaa ttg att gaa       1104
Phe Asn Pro Asn Glu Ala Glu Pro Val Ser Gln Phe Lys Leu Ile Glu           
        355                 360                 365                       

gat tct gat aat gat att gat cat gcg aaa gat gtt gat gtg aac caa       1152
Asp Ser Asp Asn Asp Ile Asp His Ala Lys Asp Val Asp Val Asn Gln           
    370                 375                 380                           

ctg gag gaa gaa gta agt aaa tca tct gac acc cta ggt ttg aca tcg       1200
Leu Glu Glu Glu Val Ser Lys Ser Ser Asp Thr Leu Gly Leu Thr Ser           
385                 390                 395                 400           

gct ccc gta cca cat gta tct aga gat aaa gat aac aaa aat ttc ttg       1248
Ala Pro Val Pro His Val Ser Arg Asp Lys Asp Asn Lys Asn Phe Leu           
                405                 410                 415               

ttt ttc cct cat ttg caa tcg cca ttt tta gta gga caa aca cag ttg       1296
Phe Phe Pro His Leu Gln Ser Pro Phe Leu Val Gly Gln Thr Gln Leu           
            420                 425                 430                   

agt aaa gta agg gct cct gga aga gaa aca atg cta tcg aac tgg gat       1344
Ser Lys Val Arg Ala Pro Gly Arg Glu Thr Met Leu Ser Asn Trp Asp           
        435                 440                 445                       

gag gag ttc tgg gct aac aga ggt aat tgg acc cgt gat atg agg cgc       1392
Glu Glu Phe Trp Ala Asn Arg Gly Asn Trp Thr Arg Asp Met Arg Arg           
    450                 455                 460                           

aaa atg aaa gat gca ttg aag cac cgt aag agg aaa caa tca aag agc       1440
Lys Met Lys Asp Ala Leu Lys His Arg Lys Arg Lys Gln Ser Lys Ser           
465                 470                 475                 480           

ggg ctt ctt cta tag                                                   1455
Gly Leu Leu Leu                                                           
                                                                          


<210>  52
<211>  484
<212>  PRT
<213>  S. cerevisiea

<400>  52

Met Asp Ser Val Ile Gln Lys Arg Ile Phe Val Gly Asn Ile Phe His 
1               5                   10                  15      


Asn Ala Asp Asp Cys Tyr Ser Glu Leu Leu Asp Arg Phe Gly Lys Phe 
            20                  25                  30          


Gly Asp Cys Gln Asp Phe Gln Phe Glu Lys His Asn His Phe Ala Phe 
        35                  40                  45              


Ile Asp Ile Arg Phe Asn Asp Glu Ala Asp Phe Asn Lys Leu Arg Lys 
    50                  55                  60                  


Ser Phe Asn Asn Val Lys Phe Lys Gly Asn Ile Leu Lys Val Asp Glu 
65                  70                  75                  80  


Ala Lys Pro Asn Trp Glu Ser Thr Trp Ala Val Gln His Ala Lys Asp 
                85                  90                  95      


Leu Lys Glu Asp Ile Ile Leu Asn Ala Lys Met Lys Lys Lys Asn Trp 
            100                 105                 110         


Gln His Tyr Lys Lys Met Glu Asn Val Ala Lys Ser Trp Lys Asp His 
        115                 120                 125             


Lys Glu Val Ile Ala Gly Arg Met Arg Glu Ala Pro Arg Lys Arg Ser 
    130                 135                 140                 


Gln Leu Arg Asn Ile Thr Phe Arg Ile Asn Val Asn Gly Ser Leu Lys 
145                 150                 155                 160 


Val Tyr Lys Cys Tyr Lys Thr Lys Leu Trp Gly Tyr Glu Arg Asn Lys 
                165                 170                 175     


Glu Leu Asn Asp Leu Val Tyr Lys Phe Thr Asn Asn Phe Trp Lys Asn 
            180                 185                 190         


Gly Tyr Asn His Ile Val Asp Arg Leu Asp Tyr Ser Arg Ala Val Lys 
        195                 200                 205             


Thr Val Arg Phe Lys Asn Gly Leu Lys Gln Leu Thr Val Ser Lys Asp 
    210                 215                 220                 


Glu Asn Val Cys Ser Gly Glu Met Asp Ser Asp Glu Asn Met Ser Glu 
225                 230                 235                 240 


Glu Glu Lys Glu Lys Asn Asn Val Ile Leu Asn Asp Leu Leu Lys Asp 
                245                 250                 255     


Phe Asp Phe Asp Lys Pro Met Thr Leu Asn Asp Ser Asp Glu Glu Leu 
            260                 265                 270         


Leu Thr Glu Gln Arg Lys Gly Glu Glu Glu Glu Glu Glu Glu Glu Glu 
        275                 280                 285             


Lys Glu Val Asn Ala Pro Glu Tyr Glu Asn Val Asn Lys Thr Lys Asp 
    290                 295                 300                 


Gln Ser Thr Leu Pro Gln Glu Lys Pro Glu Glu Arg Lys Glu Gln Asp 
305                 310                 315                 320 


Glu Gly Asp Gly Gln Glu Asp Asn Glu Phe Ile Pro Thr Phe Thr Lys 
                325                 330                 335     


Glu Ile Gly Gln Gly Thr Ile Ser Asn Thr Glu Thr Leu Arg Asn Leu 
            340                 345                 350         


Phe Asn Pro Asn Glu Ala Glu Pro Val Ser Gln Phe Lys Leu Ile Glu 
        355                 360                 365             


Asp Ser Asp Asn Asp Ile Asp His Ala Lys Asp Val Asp Val Asn Gln 
    370                 375                 380                 


Leu Glu Glu Glu Val Ser Lys Ser Ser Asp Thr Leu Gly Leu Thr Ser 
385                 390                 395                 400 


Ala Pro Val Pro His Val Ser Arg Asp Lys Asp Asn Lys Asn Phe Leu 
                405                 410                 415     


Phe Phe Pro His Leu Gln Ser Pro Phe Leu Val Gly Gln Thr Gln Leu 
            420                 425                 430         


Ser Lys Val Arg Ala Pro Gly Arg Glu Thr Met Leu Ser Asn Trp Asp 
        435                 440                 445             


Glu Glu Phe Trp Ala Asn Arg Gly Asn Trp Thr Arg Asp Met Arg Arg 
    450                 455                 460                 


Lys Met Lys Asp Ala Leu Lys His Arg Lys Arg Lys Gln Ser Lys Ser 
465                 470                 475                 480 


Gly Leu Leu Leu 
                


<210>  53
<211>  1665
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(1665)
<223>  S. cerevisiae essential gene PGI1 YBR196C SGDID:S000000400

<400>  53
atg tcc aat aac tca ttc act aac ttc aaa ctg gcc act gaa ttg cca         48
Met Ser Asn Asn Ser Phe Thr Asn Phe Lys Leu Ala Thr Glu Leu Pro           
1               5                   10                  15                

gcc tgg tct aag ttg caa aaa att tat gaa tct caa ggt aag act ttg         96
Ala Trp Ser Lys Leu Gln Lys Ile Tyr Glu Ser Gln Gly Lys Thr Leu           
            20                  25                  30                    

tct gtc aag caa gaa ttc caa aaa gat gcc aag cgt ttt gaa aaa ttg        144
Ser Val Lys Gln Glu Phe Gln Lys Asp Ala Lys Arg Phe Glu Lys Leu           
        35                  40                  45                        

aac aag act ttc acc aac tat gat ggt tcc aaa atc ttg ttc gac tac        192
Asn Lys Thr Phe Thr Asn Tyr Asp Gly Ser Lys Ile Leu Phe Asp Tyr           
    50                  55                  60                            

tca aag aac ttg gtc aac gat gaa atc att gct gca ttg att gaa ctg        240
Ser Lys Asn Leu Val Asn Asp Glu Ile Ile Ala Ala Leu Ile Glu Leu           
65                  70                  75                  80            

gcc aag gag gct aac gtc acc ggt ttg aga gat gct atg ttc aaa ggt        288
Ala Lys Glu Ala Asn Val Thr Gly Leu Arg Asp Ala Met Phe Lys Gly           
                85                  90                  95                

gaa cac atc aac tcc act gaa gat cgt gct gtc tac cac gtc gca ttg        336
Glu His Ile Asn Ser Thr Glu Asp Arg Ala Val Tyr His Val Ala Leu           
            100                 105                 110                   

aga aac aga gct aac aag cca atg tac gtt gat ggt gtc aac gtt gct        384
Arg Asn Arg Ala Asn Lys Pro Met Tyr Val Asp Gly Val Asn Val Ala           
        115                 120                 125                       

cca gaa gtc gac tct gtc ttg aag cac atg aag gag ttc tct gaa caa        432
Pro Glu Val Asp Ser Val Leu Lys His Met Lys Glu Phe Ser Glu Gln           
    130                 135                 140                           

gtt cgt tct ggt gaa tgg aag ggt tat acc ggt aag aag atc acc gat        480
Val Arg Ser Gly Glu Trp Lys Gly Tyr Thr Gly Lys Lys Ile Thr Asp           
145                 150                 155                 160           

gtt gtt aac atc ggt att ggt ggt tcc gat ttg ggt cca gtc atg gtc        528
Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Val Met Val           
                165                 170                 175               

act gag gct ttg aag cac tac gct ggt gtc ttg gat gtc cac ttc gtt        576
Thr Glu Ala Leu Lys His Tyr Ala Gly Val Leu Asp Val His Phe Val           
            180                 185                 190                   

tcc aac att gac ggt act cac att gct gaa acc ttg aag gtt gtt gac        624
Ser Asn Ile Asp Gly Thr His Ile Ala Glu Thr Leu Lys Val Val Asp           
        195                 200                 205                       

cca gaa act act ttg ttt ttg att gct tcc aag act ttc act acc gct        672
Pro Glu Thr Thr Leu Phe Leu Ile Ala Ser Lys Thr Phe Thr Thr Ala           
    210                 215                 220                           

gaa act atc act aac gct aac act gcc aag aac tgg ttc ttg tcg aag        720
Glu Thr Ile Thr Asn Ala Asn Thr Ala Lys Asn Trp Phe Leu Ser Lys           
225                 230                 235                 240           

aca ggt aat gat cca tct cac att gct aag cat ttc gct gct ttg tcc        768
Thr Gly Asn Asp Pro Ser His Ile Ala Lys His Phe Ala Ala Leu Ser           
                245                 250                 255               

act aac gaa acc gaa gtt gcc aag ttc ggt att gac acc aaa aac atg        816
Thr Asn Glu Thr Glu Val Ala Lys Phe Gly Ile Asp Thr Lys Asn Met           
            260                 265                 270                   

ttt ggt ttc gaa agt tgg gtc ggt ggt cgt tac tct gtc tgg tcg gct        864
Phe Gly Phe Glu Ser Trp Val Gly Gly Arg Tyr Ser Val Trp Ser Ala           
        275                 280                 285                       

att ggt ttg tct gtt gcc ttg tac att ggc tat gac aac ttt gag gct        912
Ile Gly Leu Ser Val Ala Leu Tyr Ile Gly Tyr Asp Asn Phe Glu Ala           
    290                 295                 300                           

ttc ttg aag ggt gct gaa gcc gtc gac aac cac ttc acc caa acc cca        960
Phe Leu Lys Gly Ala Glu Ala Val Asp Asn His Phe Thr Gln Thr Pro           
305                 310                 315                 320           

ttg gaa gac aac att cca ttg ttg ggt ggt ttg ttg tct gtc tgg tac       1008
Leu Glu Asp Asn Ile Pro Leu Leu Gly Gly Leu Leu Ser Val Trp Tyr           
                325                 330                 335               

aac aac ttc ttt ggt gct caa acc cat ttg gtt gct cca ttc gac caa       1056
Asn Asn Phe Phe Gly Ala Gln Thr His Leu Val Ala Pro Phe Asp Gln           
            340                 345                 350                   

tac ttg cac aga ttc cca gcc tac ttg caa caa ttg tca atg gaa tct       1104
Tyr Leu His Arg Phe Pro Ala Tyr Leu Gln Gln Leu Ser Met Glu Ser           
        355                 360                 365                       

aac ggt aag tct gtt acc aga ggt aac gtg ttt act gac tac tct act       1152
Asn Gly Lys Ser Val Thr Arg Gly Asn Val Phe Thr Asp Tyr Ser Thr           
    370                 375                 380                           

ggt tct atc ttg ttt ggt gaa cca gct acc aac gct caa cac tct ttc       1200
Gly Ser Ile Leu Phe Gly Glu Pro Ala Thr Asn Ala Gln His Ser Phe           
385                 390                 395                 400           

ttc caa ttg gtt cac caa ggt acc aag ttg att cca tct gat ttc atc       1248
Phe Gln Leu Val His Gln Gly Thr Lys Leu Ile Pro Ser Asp Phe Ile           
                405                 410                 415               

tta gct gct caa tct cat aac cca att gag aac aaa tta cat caa aag       1296
Leu Ala Ala Gln Ser His Asn Pro Ile Glu Asn Lys Leu His Gln Lys           
            420                 425                 430                   

atg ttg gct tca aac ttc ttt gct caa gct gaa gct tta atg gtt ggt       1344
Met Leu Ala Ser Asn Phe Phe Ala Gln Ala Glu Ala Leu Met Val Gly           
        435                 440                 445                       

aag gat gaa gaa caa gtt aag gct gaa ggt gcc act ggt ggt ttg gtc       1392
Lys Asp Glu Glu Gln Val Lys Ala Glu Gly Ala Thr Gly Gly Leu Val           
    450                 455                 460                           

cca cac aag gtc ttc tca ggt aac aga cca act acc tct atc ttg gct       1440
Pro His Lys Val Phe Ser Gly Asn Arg Pro Thr Thr Ser Ile Leu Ala           
465                 470                 475                 480           

caa aag att act cca gct act ttg ggt gct ttg att gcc tac tac gaa       1488
Gln Lys Ile Thr Pro Ala Thr Leu Gly Ala Leu Ile Ala Tyr Tyr Glu           
                485                 490                 495               

cat gtt act ttc act gaa ggt gcc att tgg aat atc aac tct ttc gac       1536
His Val Thr Phe Thr Glu Gly Ala Ile Trp Asn Ile Asn Ser Phe Asp           
            500                 505                 510                   

caa tgg ggt gtt gaa ttg ggt aaa gtc ttg gct aaa gtc atc ggc aag       1584
Gln Trp Gly Val Glu Leu Gly Lys Val Leu Ala Lys Val Ile Gly Lys           
        515                 520                 525                       

gaa ttg gac aac tcc tcc acc att tct acc cac gat gct tct acc aac       1632
Glu Leu Asp Asn Ser Ser Thr Ile Ser Thr His Asp Ala Ser Thr Asn           
    530                 535                 540                           

ggt tta atc aat caa ttc aag gaa tgg atg tga                           1665
Gly Leu Ile Asn Gln Phe Lys Glu Trp Met                                   
545                 550                                                   


<210>  54
<211>  554
<212>  PRT
<213>  S. cerevisiae

<400>  54

Met Ser Asn Asn Ser Phe Thr Asn Phe Lys Leu Ala Thr Glu Leu Pro 
1               5                   10                  15      


Ala Trp Ser Lys Leu Gln Lys Ile Tyr Glu Ser Gln Gly Lys Thr Leu 
            20                  25                  30          


Ser Val Lys Gln Glu Phe Gln Lys Asp Ala Lys Arg Phe Glu Lys Leu 
        35                  40                  45              


Asn Lys Thr Phe Thr Asn Tyr Asp Gly Ser Lys Ile Leu Phe Asp Tyr 
    50                  55                  60                  


Ser Lys Asn Leu Val Asn Asp Glu Ile Ile Ala Ala Leu Ile Glu Leu 
65                  70                  75                  80  


Ala Lys Glu Ala Asn Val Thr Gly Leu Arg Asp Ala Met Phe Lys Gly 
                85                  90                  95      


Glu His Ile Asn Ser Thr Glu Asp Arg Ala Val Tyr His Val Ala Leu 
            100                 105                 110         


Arg Asn Arg Ala Asn Lys Pro Met Tyr Val Asp Gly Val Asn Val Ala 
        115                 120                 125             


Pro Glu Val Asp Ser Val Leu Lys His Met Lys Glu Phe Ser Glu Gln 
    130                 135                 140                 


Val Arg Ser Gly Glu Trp Lys Gly Tyr Thr Gly Lys Lys Ile Thr Asp 
145                 150                 155                 160 


Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Val Met Val 
                165                 170                 175     


Thr Glu Ala Leu Lys His Tyr Ala Gly Val Leu Asp Val His Phe Val 
            180                 185                 190         


Ser Asn Ile Asp Gly Thr His Ile Ala Glu Thr Leu Lys Val Val Asp 
        195                 200                 205             


Pro Glu Thr Thr Leu Phe Leu Ile Ala Ser Lys Thr Phe Thr Thr Ala 
    210                 215                 220                 


Glu Thr Ile Thr Asn Ala Asn Thr Ala Lys Asn Trp Phe Leu Ser Lys 
225                 230                 235                 240 


Thr Gly Asn Asp Pro Ser His Ile Ala Lys His Phe Ala Ala Leu Ser 
                245                 250                 255     


Thr Asn Glu Thr Glu Val Ala Lys Phe Gly Ile Asp Thr Lys Asn Met 
            260                 265                 270         


Phe Gly Phe Glu Ser Trp Val Gly Gly Arg Tyr Ser Val Trp Ser Ala 
        275                 280                 285             


Ile Gly Leu Ser Val Ala Leu Tyr Ile Gly Tyr Asp Asn Phe Glu Ala 
    290                 295                 300                 


Phe Leu Lys Gly Ala Glu Ala Val Asp Asn His Phe Thr Gln Thr Pro 
305                 310                 315                 320 


Leu Glu Asp Asn Ile Pro Leu Leu Gly Gly Leu Leu Ser Val Trp Tyr 
                325                 330                 335     


Asn Asn Phe Phe Gly Ala Gln Thr His Leu Val Ala Pro Phe Asp Gln 
            340                 345                 350         


Tyr Leu His Arg Phe Pro Ala Tyr Leu Gln Gln Leu Ser Met Glu Ser 
        355                 360                 365             


Asn Gly Lys Ser Val Thr Arg Gly Asn Val Phe Thr Asp Tyr Ser Thr 
    370                 375                 380                 


Gly Ser Ile Leu Phe Gly Glu Pro Ala Thr Asn Ala Gln His Ser Phe 
385                 390                 395                 400 


Phe Gln Leu Val His Gln Gly Thr Lys Leu Ile Pro Ser Asp Phe Ile 
                405                 410                 415     


Leu Ala Ala Gln Ser His Asn Pro Ile Glu Asn Lys Leu His Gln Lys 
            420                 425                 430         


Met Leu Ala Ser Asn Phe Phe Ala Gln Ala Glu Ala Leu Met Val Gly 
        435                 440                 445             


Lys Asp Glu Glu Gln Val Lys Ala Glu Gly Ala Thr Gly Gly Leu Val 
    450                 455                 460                 


Pro His Lys Val Phe Ser Gly Asn Arg Pro Thr Thr Ser Ile Leu Ala 
465                 470                 475                 480 


Gln Lys Ile Thr Pro Ala Thr Leu Gly Ala Leu Ile Ala Tyr Tyr Glu 
                485                 490                 495     


His Val Thr Phe Thr Glu Gly Ala Ile Trp Asn Ile Asn Ser Phe Asp 
            500                 505                 510         


Gln Trp Gly Val Glu Leu Gly Lys Val Leu Ala Lys Val Ile Gly Lys 
        515                 520                 525             


Glu Leu Asp Asn Ser Ser Thr Ile Ser Thr His Asp Ala Ser Thr Asn 
    530                 535                 540                 


Gly Leu Ile Asn Gln Phe Lys Glu Trp Met 
545                 550                 


<210>  55
<211>  759
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(759)
<223>  S. cerevisiae essential gene NEP1 EMG1 YLR186W SGDID:S000004176

<400>  55
atg gtc gaa gat tcc aga gtt aga gac gcc ctc aaa ggt ggt gat cag         48
Met Val Glu Asp Ser Arg Val Arg Asp Ala Leu Lys Gly Gly Asp Gln           
1               5                   10                  15                

aag gca tta ccg gcc tct ttg gtt cct caa gca cct cct gtc ttg aca         96
Lys Ala Leu Pro Ala Ser Leu Val Pro Gln Ala Pro Pro Val Leu Thr           
            20                  25                  30                    

tca aag gat aag att act aag cgg atg att gtg gta tta gcg atg gca        144
Ser Lys Asp Lys Ile Thr Lys Arg Met Ile Val Val Leu Ala Met Ala           
        35                  40                  45                        

tcc ctc gag aca cac aag ata tcg tcc aac ggg cct ggt ggt gac aaa        192
Ser Leu Glu Thr His Lys Ile Ser Ser Asn Gly Pro Gly Gly Asp Lys           
    50                  55                  60                            

tat gtc ctt ttg aac tgt gac gac cat caa ggt tta tta aaa aaa atg        240
Tyr Val Leu Leu Asn Cys Asp Asp His Gln Gly Leu Leu Lys Lys Met           
65                  70                  75                  80            

ggt aga gac att agt gaa gca aga cct gat att acc cac caa tgt ctt        288
Gly Arg Asp Ile Ser Glu Ala Arg Pro Asp Ile Thr His Gln Cys Leu           
                85                  90                  95                

ttg acg ttg cta gat tct cca atc aac aaa gcc gga aag ctg cag gtc        336
Leu Thr Leu Leu Asp Ser Pro Ile Asn Lys Ala Gly Lys Leu Gln Val           
            100                 105                 110                   

tat att caa aca agt cga gga att ctg atc gag gtt aac ccc act gtt        384
Tyr Ile Gln Thr Ser Arg Gly Ile Leu Ile Glu Val Asn Pro Thr Val           
        115                 120                 125                       

cgt ata cca aga act ttc aaa aga ttt tca ggt tta atg gtt cag tta        432
Arg Ile Pro Arg Thr Phe Lys Arg Phe Ser Gly Leu Met Val Gln Leu           
    130                 135                 140                           

cta cat aag ctt tct atc aga tcg gta aat tct gaa gaa aag tta ctt        480
Leu His Lys Leu Ser Ile Arg Ser Val Asn Ser Glu Glu Lys Leu Leu           
145                 150                 155                 160           

aaa gtc att aag aac cca att acc gat cac cta cct act aag tgc cgt        528
Lys Val Ile Lys Asn Pro Ile Thr Asp His Leu Pro Thr Lys Cys Arg           
                165                 170                 175               

aag gtg aca tta tcc ttt gac gca cca gtt atc cgc gtt caa gat tac        576
Lys Val Thr Leu Ser Phe Asp Ala Pro Val Ile Arg Val Gln Asp Tyr           
            180                 185                 190                   

atc gaa aaa cta gac gat gat gaa agt ata tgt gtc ttt gtt ggt gcc        624
Ile Glu Lys Leu Asp Asp Asp Glu Ser Ile Cys Val Phe Val Gly Ala           
        195                 200                 205                       

atg gca aga ggt aaa gat aac ttt gcg gat gaa tac gtc gac gaa aaa        672
Met Ala Arg Gly Lys Asp Asn Phe Ala Asp Glu Tyr Val Asp Glu Lys           
    210                 215                 220                           

gtc ggc ttg tcc aat tac cca ttg tct gcc tca gtt gca tgt tct aaa        720
Val Gly Leu Ser Asn Tyr Pro Leu Ser Ala Ser Val Ala Cys Ser Lys           
225                 230                 235                 240           

ttt tgc cat ggc gct gaa gat gct tgg aat att tta tag                    759
Phe Cys His Gly Ala Glu Asp Ala Trp Asn Ile Leu                           
                245                 250                                   


<210>  56
<211>  252
<212>  PRT
<213>  S. cerevisiae

<400>  56

Met Val Glu Asp Ser Arg Val Arg Asp Ala Leu Lys Gly Gly Asp Gln 
1               5                   10                  15      


Lys Ala Leu Pro Ala Ser Leu Val Pro Gln Ala Pro Pro Val Leu Thr 
            20                  25                  30          


Ser Lys Asp Lys Ile Thr Lys Arg Met Ile Val Val Leu Ala Met Ala 
        35                  40                  45              


Ser Leu Glu Thr His Lys Ile Ser Ser Asn Gly Pro Gly Gly Asp Lys 
    50                  55                  60                  


Tyr Val Leu Leu Asn Cys Asp Asp His Gln Gly Leu Leu Lys Lys Met 
65                  70                  75                  80  


Gly Arg Asp Ile Ser Glu Ala Arg Pro Asp Ile Thr His Gln Cys Leu 
                85                  90                  95      


Leu Thr Leu Leu Asp Ser Pro Ile Asn Lys Ala Gly Lys Leu Gln Val 
            100                 105                 110         


Tyr Ile Gln Thr Ser Arg Gly Ile Leu Ile Glu Val Asn Pro Thr Val 
        115                 120                 125             


Arg Ile Pro Arg Thr Phe Lys Arg Phe Ser Gly Leu Met Val Gln Leu 
    130                 135                 140                 


Leu His Lys Leu Ser Ile Arg Ser Val Asn Ser Glu Glu Lys Leu Leu 
145                 150                 155                 160 


Lys Val Ile Lys Asn Pro Ile Thr Asp His Leu Pro Thr Lys Cys Arg 
                165                 170                 175     


Lys Val Thr Leu Ser Phe Asp Ala Pro Val Ile Arg Val Gln Asp Tyr 
            180                 185                 190         


Ile Glu Lys Leu Asp Asp Asp Glu Ser Ile Cys Val Phe Val Gly Ala 
        195                 200                 205             


Met Ala Arg Gly Lys Asp Asn Phe Ala Asp Glu Tyr Val Asp Glu Lys 
    210                 215                 220                 


Val Gly Leu Ser Asn Tyr Pro Leu Ser Ala Ser Val Ala Cys Ser Lys 
225                 230                 235                 240 


Phe Cys His Gly Ala Glu Asp Ala Trp Asn Ile Leu 
                245                 250         


<210>  57
<211>  804
<212>  DNA
<213>  S. cerevisiea


<220>
<221>  CDS
<222>  (1)..(804)
<223>  S. cerevisea essential gene URA3

<400>  57
atg tcg aaa gct aca tat aag gaa cgt gct gct act cat cct agt cct         48
Met Ser Lys Ala Thr Tyr Lys Glu Arg Ala Ala Thr His Pro Ser Pro           
1               5                   10                  15                

gtt gct gcc aag cta ttt aat atc atg cac gaa aag caa aca aac ttg         96
Val Ala Ala Lys Leu Phe Asn Ile Met His Glu Lys Gln Thr Asn Leu           
            20                  25                  30                    

tgt gct tca ttg gat gtt cgt acc acc aag gaa tta ctg gag tta gtt        144
Cys Ala Ser Leu Asp Val Arg Thr Thr Lys Glu Leu Leu Glu Leu Val           
        35                  40                  45                        

gaa gca tta ggt ccc aaa att tgt tta cta aaa aca cat gtg gat atc        192
Glu Ala Leu Gly Pro Lys Ile Cys Leu Leu Lys Thr His Val Asp Ile           
    50                  55                  60                            

ttg act gat ttt tcc atg gag ggc aca gtt aag ccg cta aag gca tta        240
Leu Thr Asp Phe Ser Met Glu Gly Thr Val Lys Pro Leu Lys Ala Leu           
65                  70                  75                  80            

tcc gcc aag tac aat ttt tta ctc ttc gaa gac aga aaa ttt gct gac        288
Ser Ala Lys Tyr Asn Phe Leu Leu Phe Glu Asp Arg Lys Phe Ala Asp           
                85                  90                  95                

att ggt aat aca gtc aaa ttg cag tac tct gcg ggt gta tac aga ata        336
Ile Gly Asn Thr Val Lys Leu Gln Tyr Ser Ala Gly Val Tyr Arg Ile           
            100                 105                 110                   

gca gaa tgg gca gac att acg aat gca cac ggt gtg gtg ggc cca ggt        384
Ala Glu Trp Ala Asp Ile Thr Asn Ala His Gly Val Val Gly Pro Gly           
        115                 120                 125                       

att gtt agc ggt ttg aag cag gcg gca gaa gaa gta aca aag gaa cct        432
Ile Val Ser Gly Leu Lys Gln Ala Ala Glu Glu Val Thr Lys Glu Pro           
    130                 135                 140                           

aga ggc ctt ttg atg tta gca gaa ttg tca tgc aag ggc tcc cta tct        480
Arg Gly Leu Leu Met Leu Ala Glu Leu Ser Cys Lys Gly Ser Leu Ser           
145                 150                 155                 160           

act gga gaa tat act aag ggt act gtt gac att gcg aag agc gac aaa        528
Thr Gly Glu Tyr Thr Lys Gly Thr Val Asp Ile Ala Lys Ser Asp Lys           
                165                 170                 175               

gat ttt gtt atc ggc ttt att gct caa aga gac atg ggt gga aga gat        576
Asp Phe Val Ile Gly Phe Ile Ala Gln Arg Asp Met Gly Gly Arg Asp           
            180                 185                 190                   

gaa ggt tac gat tgg ttg att atg aca ccc ggt gtg ggt tta gat gac        624
Glu Gly Tyr Asp Trp Leu Ile Met Thr Pro Gly Val Gly Leu Asp Asp           
        195                 200                 205                       

aag gga gac gca ttg ggt caa cag tat aga acc gtg gat gat gtg gtc        672
Lys Gly Asp Ala Leu Gly Gln Gln Tyr Arg Thr Val Asp Asp Val Val           
    210                 215                 220                           

tct aca gga tct gac att att att gtt gga aga gga cta ttt gca aag        720
Ser Thr Gly Ser Asp Ile Ile Ile Val Gly Arg Gly Leu Phe Ala Lys           
225                 230                 235                 240           

gga agg gat gct aag gta gag ggt gaa cgt tac aga aaa gca ggc tgg        768
Gly Arg Asp Ala Lys Val Glu Gly Glu Arg Tyr Arg Lys Ala Gly Trp           
                245                 250                 255               

gaa gca tat ttg aga aga tgc ggc cag caa aac taa                        804
Glu Ala Tyr Leu Arg Arg Cys Gly Gln Gln Asn                               
            260                 265                                       


<210>  58
<211>  267
<212>  PRT
<213>  S. cerevisiea

<400>  58

Met Ser Lys Ala Thr Tyr Lys Glu Arg Ala Ala Thr His Pro Ser Pro 
1               5                   10                  15      


Val Ala Ala Lys Leu Phe Asn Ile Met His Glu Lys Gln Thr Asn Leu 
            20                  25                  30          


Cys Ala Ser Leu Asp Val Arg Thr Thr Lys Glu Leu Leu Glu Leu Val 
        35                  40                  45              


Glu Ala Leu Gly Pro Lys Ile Cys Leu Leu Lys Thr His Val Asp Ile 
    50                  55                  60                  


Leu Thr Asp Phe Ser Met Glu Gly Thr Val Lys Pro Leu Lys Ala Leu 
65                  70                  75                  80  


Ser Ala Lys Tyr Asn Phe Leu Leu Phe Glu Asp Arg Lys Phe Ala Asp 
                85                  90                  95      


Ile Gly Asn Thr Val Lys Leu Gln Tyr Ser Ala Gly Val Tyr Arg Ile 
            100                 105                 110         


Ala Glu Trp Ala Asp Ile Thr Asn Ala His Gly Val Val Gly Pro Gly 
        115                 120                 125             


Ile Val Ser Gly Leu Lys Gln Ala Ala Glu Glu Val Thr Lys Glu Pro 
    130                 135                 140                 


Arg Gly Leu Leu Met Leu Ala Glu Leu Ser Cys Lys Gly Ser Leu Ser 
145                 150                 155                 160 


Thr Gly Glu Tyr Thr Lys Gly Thr Val Asp Ile Ala Lys Ser Asp Lys 
                165                 170                 175     


Asp Phe Val Ile Gly Phe Ile Ala Gln Arg Asp Met Gly Gly Arg Asp 
            180                 185                 190         


Glu Gly Tyr Asp Trp Leu Ile Met Thr Pro Gly Val Gly Leu Asp Asp 
        195                 200                 205             


Lys Gly Asp Ala Leu Gly Gln Gln Tyr Arg Thr Val Asp Asp Val Val 
    210                 215                 220                 


Ser Thr Gly Ser Asp Ile Ile Ile Val Gly Arg Gly Leu Phe Ala Lys 
225                 230                 235                 240 


Gly Arg Asp Ala Lys Val Glu Gly Glu Arg Tyr Arg Lys Ala Gly Trp 
                245                 250                 255     


Glu Ala Tyr Leu Arg Arg Cys Gly Gln Gln Asn 
            260                 265         


<210>  59
<211>  1095
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(1095)
<223>  S. ceverisiae essential gene LEU2

<400>  59
atg tct gcc cct aag aag atc gtc gtt ttg cca ggt gac cac gtt ggt         48
Met Ser Ala Pro Lys Lys Ile Val Val Leu Pro Gly Asp His Val Gly           
1               5                   10                  15                

caa gaa atc aca gcc gaa gcc att aag gtt ctt aaa gct att tct gat         96
Gln Glu Ile Thr Ala Glu Ala Ile Lys Val Leu Lys Ala Ile Ser Asp           
            20                  25                  30                    

gtt cgt tcc aat gtc aag ttc gat ttc gaa aat cat tta att ggt ggt        144
Val Arg Ser Asn Val Lys Phe Asp Phe Glu Asn His Leu Ile Gly Gly           
        35                  40                  45                        

gct gct atc gat gct aca ggt gtc cca ctt cca gat gag gcg ctg gaa        192
Ala Ala Ile Asp Ala Thr Gly Val Pro Leu Pro Asp Glu Ala Leu Glu           
    50                  55                  60                            

gcc tcc aag aag gtt gat gcc gtt ttg tta ggt gct gtg gct ggt cct        240
Ala Ser Lys Lys Val Asp Ala Val Leu Leu Gly Ala Val Ala Gly Pro           
65                  70                  75                  80            

aaa tgg ggt acc ggt agt gtt aga cct gaa caa ggt tta cta aaa atc        288
Lys Trp Gly Thr Gly Ser Val Arg Pro Glu Gln Gly Leu Leu Lys Ile           
                85                  90                  95                

cgt aaa gaa ctt caa ttg tac gcc aac tta aga cca tgt aac ttt gca        336
Arg Lys Glu Leu Gln Leu Tyr Ala Asn Leu Arg Pro Cys Asn Phe Ala           
            100                 105                 110                   

tcc gac tct ctt tta gac tta tct cca atc aag cca caa ttt gct aaa        384
Ser Asp Ser Leu Leu Asp Leu Ser Pro Ile Lys Pro Gln Phe Ala Lys           
        115                 120                 125                       

ggt act gac ttc gtt gtt gtc aga gaa tta gtg gga ggt att tac ttt        432
Gly Thr Asp Phe Val Val Val Arg Glu Leu Val Gly Gly Ile Tyr Phe           
    130                 135                 140                           

ggt aag aga aag gaa gac gat ggt gat ggt gtc gct tgg gat agt gaa        480
Gly Lys Arg Lys Glu Asp Asp Gly Asp Gly Val Ala Trp Asp Ser Glu           
145                 150                 155                 160           

caa tac acc gtt cca gaa gtg caa aga atc aca aga atg gcc gct ttc        528
Gln Tyr Thr Val Pro Glu Val Gln Arg Ile Thr Arg Met Ala Ala Phe           
                165                 170                 175               

atg gcc cta caa cat gag cca cca ttg cct att tgg tcc ttg gat aaa        576
Met Ala Leu Gln His Glu Pro Pro Leu Pro Ile Trp Ser Leu Asp Lys           
            180                 185                 190                   

gct aat ctt ttg gcc tct tca aga tta tgg aga aaa act gtg gag gaa        624
Ala Asn Leu Leu Ala Ser Ser Arg Leu Trp Arg Lys Thr Val Glu Glu           
        195                 200                 205                       

acc atc aag aac gaa ttc cct aca ttg aag gtt caa cat caa ttg att        672
Thr Ile Lys Asn Glu Phe Pro Thr Leu Lys Val Gln His Gln Leu Ile           
    210                 215                 220                           

gat tct gcc gcc atg atc cta gtt aag aac cca acc cac cta aat ggt        720
Asp Ser Ala Ala Met Ile Leu Val Lys Asn Pro Thr His Leu Asn Gly           
225                 230                 235                 240           

att ata atc acc agc aac atg ttt ggt gat atc atc tcc gat gaa gcc        768
Ile Ile Ile Thr Ser Asn Met Phe Gly Asp Ile Ile Ser Asp Glu Ala           
                245                 250                 255               

tcc gtt atc cca ggt tcc ttg ggt ttg ttg cca tct gcg tcc ttg gcc        816
Ser Val Ile Pro Gly Ser Leu Gly Leu Leu Pro Ser Ala Ser Leu Ala           
            260                 265                 270                   

tct ttg cca gac aag aac acc gca ttt ggt ttg tac gaa cca tgc cac        864
Ser Leu Pro Asp Lys Asn Thr Ala Phe Gly Leu Tyr Glu Pro Cys His           
        275                 280                 285                       

ggt tct gct cca gat ttg cca aag aat aag gtt gac cct atc gcc act        912
Gly Ser Ala Pro Asp Leu Pro Lys Asn Lys Val Asp Pro Ile Ala Thr           
    290                 295                 300                           

atc ttg tct gct gca atg atg ttg aaa ttg tca ttg aac ttg cct gaa        960
Ile Leu Ser Ala Ala Met Met Leu Lys Leu Ser Leu Asn Leu Pro Glu           
305                 310                 315                 320           

gaa ggt aag gcc att gaa gat gca gtt aaa aag gtt ttg gat gca ggt       1008
Glu Gly Lys Ala Ile Glu Asp Ala Val Lys Lys Val Leu Asp Ala Gly           
                325                 330                 335               

atc aga act ggt gat tta ggt ggt tcc aac agt acc acc gaa gtc ggt       1056
Ile Arg Thr Gly Asp Leu Gly Gly Ser Asn Ser Thr Thr Glu Val Gly           
            340                 345                 350                   

gat gct gtc gcc gaa gaa gtt aag aaa atc ctt gct taa                   1095
Asp Ala Val Ala Glu Glu Val Lys Lys Ile Leu Ala                           
        355                 360                                           


<210>  60
<211>  364
<212>  PRT
<213>  S. cerevisiae

<400>  60

Met Ser Ala Pro Lys Lys Ile Val Val Leu Pro Gly Asp His Val Gly 
1               5                   10                  15      


Gln Glu Ile Thr Ala Glu Ala Ile Lys Val Leu Lys Ala Ile Ser Asp 
            20                  25                  30          


Val Arg Ser Asn Val Lys Phe Asp Phe Glu Asn His Leu Ile Gly Gly 
        35                  40                  45              


Ala Ala Ile Asp Ala Thr Gly Val Pro Leu Pro Asp Glu Ala Leu Glu 
    50                  55                  60                  


Ala Ser Lys Lys Val Asp Ala Val Leu Leu Gly Ala Val Ala Gly Pro 
65                  70                  75                  80  


Lys Trp Gly Thr Gly Ser Val Arg Pro Glu Gln Gly Leu Leu Lys Ile 
                85                  90                  95      


Arg Lys Glu Leu Gln Leu Tyr Ala Asn Leu Arg Pro Cys Asn Phe Ala 
            100                 105                 110         


Ser Asp Ser Leu Leu Asp Leu Ser Pro Ile Lys Pro Gln Phe Ala Lys 
        115                 120                 125             


Gly Thr Asp Phe Val Val Val Arg Glu Leu Val Gly Gly Ile Tyr Phe 
    130                 135                 140                 


Gly Lys Arg Lys Glu Asp Asp Gly Asp Gly Val Ala Trp Asp Ser Glu 
145                 150                 155                 160 


Gln Tyr Thr Val Pro Glu Val Gln Arg Ile Thr Arg Met Ala Ala Phe 
                165                 170                 175     


Met Ala Leu Gln His Glu Pro Pro Leu Pro Ile Trp Ser Leu Asp Lys 
            180                 185                 190         


Ala Asn Leu Leu Ala Ser Ser Arg Leu Trp Arg Lys Thr Val Glu Glu 
        195                 200                 205             


Thr Ile Lys Asn Glu Phe Pro Thr Leu Lys Val Gln His Gln Leu Ile 
    210                 215                 220                 


Asp Ser Ala Ala Met Ile Leu Val Lys Asn Pro Thr His Leu Asn Gly 
225                 230                 235                 240 


Ile Ile Ile Thr Ser Asn Met Phe Gly Asp Ile Ile Ser Asp Glu Ala 
                245                 250                 255     


Ser Val Ile Pro Gly Ser Leu Gly Leu Leu Pro Ser Ala Ser Leu Ala 
            260                 265                 270         


Ser Leu Pro Asp Lys Asn Thr Ala Phe Gly Leu Tyr Glu Pro Cys His 
        275                 280                 285             


Gly Ser Ala Pro Asp Leu Pro Lys Asn Lys Val Asp Pro Ile Ala Thr 
    290                 295                 300                 


Ile Leu Ser Ala Ala Met Met Leu Lys Leu Ser Leu Asn Leu Pro Glu 
305                 310                 315                 320 


Glu Gly Lys Ala Ile Glu Asp Ala Val Lys Lys Val Leu Asp Ala Gly 
                325                 330                 335     


Ile Arg Thr Gly Asp Leu Gly Gly Ser Asn Ser Thr Thr Glu Val Gly 
            340                 345                 350         


Asp Ala Val Ala Glu Glu Val Lys Lys Ile Leu Ala 
        355                 360                 


<210>  61
<211>  675
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(675)
<223>  S. cerevisiae essential gene TRP1

<400>  61
atg tct gtt att aat ttc aca ggt agt tct ggt cca ttg gtg aaa gtt         48
Met Ser Val Ile Asn Phe Thr Gly Ser Ser Gly Pro Leu Val Lys Val           
1               5                   10                  15                

tgc ggc ttg cag agc aca gag gcc gca gaa tgt gct cta gat tcc gat         96
Cys Gly Leu Gln Ser Thr Glu Ala Ala Glu Cys Ala Leu Asp Ser Asp           
            20                  25                  30                    

gct gac ttg ctg ggt att ata tgt gtg ccc aat aga aag aga aca att        144
Ala Asp Leu Leu Gly Ile Ile Cys Val Pro Asn Arg Lys Arg Thr Ile           
        35                  40                  45                        

gac ccg gtt att gca agg aaa att tca agt ctt gta aaa gca tat aaa        192
Asp Pro Val Ile Ala Arg Lys Ile Ser Ser Leu Val Lys Ala Tyr Lys           
    50                  55                  60                            

aat agt tca ggc act ccg aaa tac ttg gtt ggc gtg ttt cgt aat caa        240
Asn Ser Ser Gly Thr Pro Lys Tyr Leu Val Gly Val Phe Arg Asn Gln           
65                  70                  75                  80            

cct aag gag gat gtt ttg gct ctg gtc aat gat tac ggc att gat atc        288
Pro Lys Glu Asp Val Leu Ala Leu Val Asn Asp Tyr Gly Ile Asp Ile           
                85                  90                  95                

gtc caa ctg cat gga gat gag tcg tgg caa gaa tac caa gag ttc ctc        336
Val Gln Leu His Gly Asp Glu Ser Trp Gln Glu Tyr Gln Glu Phe Leu           
            100                 105                 110                   

ggt ttg cca gtt att aaa aga ctc gta ttt cca aaa gac tgc aac ata        384
Gly Leu Pro Val Ile Lys Arg Leu Val Phe Pro Lys Asp Cys Asn Ile           
        115                 120                 125                       

cta ctc agt gca gct tca cag aaa cct cat tcg ttt att ccc ttg ttt        432
Leu Leu Ser Ala Ala Ser Gln Lys Pro His Ser Phe Ile Pro Leu Phe           
    130                 135                 140                           

gat tca gaa gca ggt ggg aca ggt gaa ctt ttg gat tgg aac tcg att        480
Asp Ser Glu Ala Gly Gly Thr Gly Glu Leu Leu Asp Trp Asn Ser Ile           
145                 150                 155                 160           

tct gac tgg gtt gga agg caa gag agc ccc gaa agc tta cat ttt atg        528
Ser Asp Trp Val Gly Arg Gln Glu Ser Pro Glu Ser Leu His Phe Met           
                165                 170                 175               

tta gct ggt gga ctg acg cca gaa aat gtt ggt gat gcg ctt aga tta        576
Leu Ala Gly Gly Leu Thr Pro Glu Asn Val Gly Asp Ala Leu Arg Leu           
            180                 185                 190                   

aat ggc gtt att ggt gtt gat gta agc gga ggt gtg gag aca aat ggt        624
Asn Gly Val Ile Gly Val Asp Val Ser Gly Gly Val Glu Thr Asn Gly           
        195                 200                 205                       

gta aaa gac tct aac aaa ata gca aat ttc gtc aaa aat gct aag aaa        672
Val Lys Asp Ser Asn Lys Ile Ala Asn Phe Val Lys Asn Ala Lys Lys           
    210                 215                 220                           

tag                                                                    675


<210>  62
<211>  224
<212>  PRT
<213>  S. cerevisiae

<400>  62

Met Ser Val Ile Asn Phe Thr Gly Ser Ser Gly Pro Leu Val Lys Val 
1               5                   10                  15      


Cys Gly Leu Gln Ser Thr Glu Ala Ala Glu Cys Ala Leu Asp Ser Asp 
            20                  25                  30          


Ala Asp Leu Leu Gly Ile Ile Cys Val Pro Asn Arg Lys Arg Thr Ile 
        35                  40                  45              


Asp Pro Val Ile Ala Arg Lys Ile Ser Ser Leu Val Lys Ala Tyr Lys 
    50                  55                  60                  


Asn Ser Ser Gly Thr Pro Lys Tyr Leu Val Gly Val Phe Arg Asn Gln 
65                  70                  75                  80  


Pro Lys Glu Asp Val Leu Ala Leu Val Asn Asp Tyr Gly Ile Asp Ile 
                85                  90                  95      


Val Gln Leu His Gly Asp Glu Ser Trp Gln Glu Tyr Gln Glu Phe Leu 
            100                 105                 110         


Gly Leu Pro Val Ile Lys Arg Leu Val Phe Pro Lys Asp Cys Asn Ile 
        115                 120                 125             


Leu Leu Ser Ala Ala Ser Gln Lys Pro His Ser Phe Ile Pro Leu Phe 
    130                 135                 140                 


Asp Ser Glu Ala Gly Gly Thr Gly Glu Leu Leu Asp Trp Asn Ser Ile 
145                 150                 155                 160 


Ser Asp Trp Val Gly Arg Gln Glu Ser Pro Glu Ser Leu His Phe Met 
                165                 170                 175     


Leu Ala Gly Gly Leu Thr Pro Glu Asn Val Gly Asp Ala Leu Arg Leu 
            180                 185                 190         


Asn Gly Val Ile Gly Val Asp Val Ser Gly Gly Val Glu Thr Asn Gly 
        195                 200                 205             


Val Lys Asp Ser Asn Lys Ile Ala Asn Phe Val Lys Asn Ala Lys Lys 
    210                 215                 220                 


<210>  63
<211>  657
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  CDS
<222>  (1)..(657)
<223>  S. cerevisiae essential gene HIS3

<400>  63
atg aca gag cag aaa gcc cta gta aag cgt att aca aat gaa acc aag         48
Met Thr Glu Gln Lys Ala Leu Val Lys Arg Ile Thr Asn Glu Thr Lys           
1               5                   10                  15                

att cag att gcg atc tct tta aag ggt ggt ccc cta gcg ata gag cac         96
Ile Gln Ile Ala Ile Ser Leu Lys Gly Gly Pro Leu Ala Ile Glu His           
            20                  25                  30                    

tcg atc ttc cca gaa aaa gag gca gaa gca gta gca gaa cag gcc aca        144
Ser Ile Phe Pro Glu Lys Glu Ala Glu Ala Val Ala Glu Gln Ala Thr           
        35                  40                  45                        

caa tcg caa gtg att aac gtc cac aca ggt ata ggg ttt ctg gac cat        192
Gln Ser Gln Val Ile Asn Val His Thr Gly Ile Gly Phe Leu Asp His           
    50                  55                  60                            

atg ata cat gct ctg gcc aag cat tcc ggc tgg tcg cta atc gtt gag        240
Met Ile His Ala Leu Ala Lys His Ser Gly Trp Ser Leu Ile Val Glu           
65                  70                  75                  80            

tgc att ggt gac tta cac ata gac gac cat cac acc act gaa gac tgc        288
Cys Ile Gly Asp Leu His Ile Asp Asp His His Thr Thr Glu Asp Cys           
                85                  90                  95                

ggg att gct ctc ggt caa gct ttt aaa gag gcc cta ctg gcg cgt gga        336
Gly Ile Ala Leu Gly Gln Ala Phe Lys Glu Ala Leu Leu Ala Arg Gly           
            100                 105                 110                   

gta aaa agg ttt gga tca gga ttt gcg cct ttg gat gag gca ctt tcc        384
Val Lys Arg Phe Gly Ser Gly Phe Ala Pro Leu Asp Glu Ala Leu Ser           
        115                 120                 125                       

aga gcg gtg gta gat ctt tcg aac agg ccg tac gca gtt gtc gaa ctt        432
Arg Ala Val Val Asp Leu Ser Asn Arg Pro Tyr Ala Val Val Glu Leu           
    130                 135                 140                           

ggt ttg caa agg gag aaa gta gga gat ctc tct tgc gag atg atc ccg        480
Gly Leu Gln Arg Glu Lys Val Gly Asp Leu Ser Cys Glu Met Ile Pro           
145                 150                 155                 160           

cat ttt ctt gaa agc ttt gca gag gct agc aga att acc ctc cac gtt        528
His Phe Leu Glu Ser Phe Ala Glu Ala Ser Arg Ile Thr Leu His Val           
                165                 170                 175               

gat tgt ctg cga ggc aag aat gat cat cac cgt agt gag agt gcg ttc        576
Asp Cys Leu Arg Gly Lys Asn Asp His His Arg Ser Glu Ser Ala Phe           
            180                 185                 190                   

aag gct ctt gcg gtt gcc ata aga gaa gcc acc tcg ccc aat ggt acc        624
Lys Ala Leu Ala Val Ala Ile Arg Glu Ala Thr Ser Pro Asn Gly Thr           
        195                 200                 205                       

aac gat gtt ccc tcc acc aaa ggt gtt ctt atg                            657
Asn Asp Val Pro Ser Thr Lys Gly Val Leu Met                               
    210                 215                                               


<210>  64
<211>  219
<212>  PRT
<213>  S. cerevisiae

<400>  64

Met Thr Glu Gln Lys Ala Leu Val Lys Arg Ile Thr Asn Glu Thr Lys 
1               5                   10                  15      


Ile Gln Ile Ala Ile Ser Leu Lys Gly Gly Pro Leu Ala Ile Glu His 
            20                  25                  30          


Ser Ile Phe Pro Glu Lys Glu Ala Glu Ala Val Ala Glu Gln Ala Thr 
        35                  40                  45              


Gln Ser Gln Val Ile Asn Val His Thr Gly Ile Gly Phe Leu Asp His 
    50                  55                  60                  


Met Ile His Ala Leu Ala Lys His Ser Gly Trp Ser Leu Ile Val Glu 
65                  70                  75                  80  


Cys Ile Gly Asp Leu His Ile Asp Asp His His Thr Thr Glu Asp Cys 
                85                  90                  95      


Gly Ile Ala Leu Gly Gln Ala Phe Lys Glu Ala Leu Leu Ala Arg Gly 
            100                 105                 110         


Val Lys Arg Phe Gly Ser Gly Phe Ala Pro Leu Asp Glu Ala Leu Ser 
        115                 120                 125             


Arg Ala Val Val Asp Leu Ser Asn Arg Pro Tyr Ala Val Val Glu Leu 
    130                 135                 140                 


Gly Leu Gln Arg Glu Lys Val Gly Asp Leu Ser Cys Glu Met Ile Pro 
145                 150                 155                 160 


His Phe Leu Glu Ser Phe Ala Glu Ala Ser Arg Ile Thr Leu His Val 
                165                 170                 175     


Asp Cys Leu Arg Gly Lys Asn Asp His His Arg Ser Glu Ser Ala Phe 
            180                 185                 190         


Lys Ala Leu Ala Val Ala Ile Arg Glu Ala Thr Ser Pro Asn Gly Thr 
        195                 200                 205             


Asn Asp Val Pro Ser Thr Lys Gly Val Leu Met 
    210                 215                 


<210>  65
<211>  290
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(290)
<223>  burden sensing promoter p(htpG)

<400>  65
gccaccaacc ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg       60

caatatgacg tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa      120

atggtcgatg gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa      180

gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct      240

ccctcatcct gtttttaacc ttaaaatggc attattgagg tagacctaca                 290


<210>  66
<211>  257
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(257)
<223>  burden sensing promoter p(ibpA)

<400>  66
atcccttgct gaaaataaca tcatcattac gtcgcactgt ggcggctatc gcactttaac       60

gtttcgtgct gccccctcag tctatgcaat agaccataaa ctgcaaaaaa aagtccgctg      120

ataaggcttg aaaagttcat ttccagaccc atttttacat cgtagccgat gaggacgcgc      180

ctgatgggtg ttctggctac ctgacctgtc cattgtggaa ggtcttacat tctcgctgat      240

ttcaggagct attgatt                                                     257


<210>  67
<211>  298
<212>  DNA
<213>  E.coli


<220>
<221>  promoter
<222>  (1)..(298)
<223>  burden sensing promoter p(clpB)

<400>  67
gataagtatc tggcggatat ttatctgctt gcccggcagc gtctggcgag cgttggtgtt       60

gaacaaattt tcggcggcga ccgttgtaca tatacggaaa atgagacttt cttctcttat      120

cgtcgcgaca agaccaccgg gcgtatggca agtttcattt ggctgatata acctaaagaa      180

tcaagacgat ccggtacgcg tgattttctt ttcacattaa tctggtcaat aaccttgaat      240

aattgaggga tgacctcatt taatctccag tagcaacttt gatccgttat gggaggag        298


<210>  68
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(yccV)

<400>  68
ggttataaag acatcaacat gctggcgatt cagctgctga atgaaggcgg tattctcctg       60

actttctcct gttccggtct gatgaccagc gatttatttc agaaaatcat cgcggatgcc      120

gcaattgatg ccggtcgtga tgtacaattt atagagcagt tccgtcaggc agccgatcat      180

ccggtgatcg ctacctatcc ggaagggcta tatctgaaag ggtttgcctg tcgcgtcatg      240

taacttgaaa tgtggaattt tacccttata tcaaaggtaa agatttcccg ggaggtgact      300


<210>  69
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(grpE)

<400>  69
tctccgcgag cgtgccagtt ttcacattct tcagttgcag ttcgtgagcg atttgttgct       60

caacgatgac ctcgtaacct tttgtgcaca gccagcggta gagcatttca tgtgttgtca      120

gtgcagtggg gtgccgtggg tgtcccacaa tgccaataca cttgaaatga ttattcattt      180

ttccgaggtc cttgttgcga agattgatga caatgtgagt gcttcccttg aaaccctgaa      240

actgatcccc ataataagcg aagttagcga gatgaatgcg aaaaaaacgc ggagaaattc      300


<210>  70
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(ycjX)

<400>  70
ttggctgata ctgtaattct tcaatggccc gtaacacttt ttcgcgcgct tccggggaga       60

tattagtttg cttattcagc acgcgtgata cggtggattt tgatacgcct gcaaccctgg      120

caatatcata aatagtaggg gacataggtc aggaactccg tccgaacgtg aatgctgcac      180

atcttatgga gttcgccggt cgatgacaac acgggcagtt gataatcaat ggcctggccc      240

ccacattcat atccttacga atgatttttt ttctggtctt cagagcagca caggacagca      300


<210>  71
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(ldhA)

<400>  71
gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa tcagtaataa       60

cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg atttagcgaa      120

aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga aggttgcgcc      180

tacactaagc atagttgttg atgaattttt caatatcgcc atagctttca attatatttg      240

aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg agaaagtctt      300


<210>  72
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(mutM)

<400>  72
ggcgaaaagt actaagtact tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa       60

tcaagctggt ttcttctgct ggtactggtc acttctatac cactacgaag aacaaacgta      120

ctaagccgga aaaactggaa ctgaaaaaat tcgatccagt tgttcgccag cacgtgatct      180

acaaagaagc gaaaatcaaa taattctcgc tttgatgtaa caaaaaacct cgctccggcg      240

gggttttttg ttatctgctt gcccccatat tgactgcatc tgttcattcc tggagatgct      300


<210>  73
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(ybbN)

<400>  73
cccattcgta ctcgcttcac cgacaacgtc aaccagacgc aaagtgataa accagtcgaa       60

aatcccggca tcgccgcccg ctttacgttg ggaccggaag cggtggtgga caaagtacgc      120

catgctttta ttagcgagaa gccgaagatg cgctatccgg taacgctggt gacctgggca      180

gtaatggtgc ttaagcgcct gctgccgggg cgcgtgatgg acaaaatatt gcaggggtga      240

gttgaagcgc gcgcttaagc ccccatgtca aagaaaatat cgacaacaga gagcgactcc      300


<210>  74
<211>  298
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(298)
<223>  burden sensing promoter p(prlC)

<400>  74
agttcttgtt tgagtccact catgggttga tgtccgattg cgcccaaatt ttgggcaact       60

gcgtagattt tcgatggtag cacaatcaga ttcgcttatg acggtgatga agaaattgcg      120

atgaaatgtg aggtgaatca gggttttcac ccgattttgt gctgatcaga attttttttc      180

tttttccccc ttgaaggggc gaagcctcat ccccatttct ctggtcacca gccgggaaac      240

cacgtaagct ccggcgtcac ccataacaga tacggacttt ctcaaaggag agttatca        298


<210>  75
<211>  298
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(298)
<223>  burden sensing promoter p(groES)

<400>  75
agttcttgtt tgagtccact catgggttga tgtccgattg cgcccaaatt ttgggcaact       60

gcgtagattt tcgatggtag cacaatcaga ttcgcttatg acggtgatga agaaattgcg      120

atgaaatgtg aggtgaatca gggttttcac ccgattttgt gctgatcaga attttttttc      180

tttttccccc ttgaaggggc gaagcctcat ccccatttct ctggtcacca gccgggaaac      240

cacgtaagct ccggcgtcac ccataacaga tacggacttt ctcaaaggag agttatca        298


<210>  76
<211>  392
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(392)
<223>  burden sensing promoter p(fxsA)

<220>
<221>  misc_feature
<222>  (328)..(328)
<223>  n is a, c, g, or t

<400>  76
gatccccgga atattcatat gttaaacaca cagaatatat gtggtttcga atgtttttcg       60

accgacgatt atcccctgca tcgaccgaat acccgagatc atatgctgct tgaggatttc      120

taccgtaatc tggatcactt taagtgtcgg tttttacccc ttaattatta atttgtgaaa      180

tagatcaccg ctttgggatt actaccaaaa atagttgcgc aaacatcttg aaattttgct      240

aatgaccaca atataagctg aacgcgattc gcaacccatt caggtagccg ggttttaccg      300

gctgctatta caggagaaac ctacttgnaa ggtatgaaac tctttgccca gggtacttca      360

ctggacctta gccatcctca cgtaatgggg at                                    392


<210>  77
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(htpX)

<400>  77
agattaccag gagccggatc cttatctgga tgagacggtg aatatcgcac tcgatctggc       60

gaagcttgaa aaagccagac ccgcggaaca acccgctccc gtcaagtaat atcaatcagg      120

cacaagaaat tgtgcctgat tttttaacag cggcaagatg ccgtaaatca gatgctacaa      180

aatgtaaagt tgtgtctttc tggtgactta cgcactatcc agacttgaaa atagtcgcgt      240

aacccatacg atgtgggtat cgcatattgc gttttgttaa actgaggtaa aaagaaaatt      300


<210>  78
<211>  275
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(275)
<223>  burden sensing promoter p(rrnB)

<400>  78
gcaaacacgc cgccgggtca gcggggttct cctgagaatt ccggcagaga aagcaaaaat       60

aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaag      120

cgaagcggca ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg      180

gattcttaac gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa cacgtaattc      240

attacgaagt ttaattcttt gagcgtcaaa ctttt                                 275


<210>  79
<211>  296
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(296)
<223>  burden sensing promoter p(rrnE)

<400>  79
aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg aacaacgaag       60

agaaaaaaat cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc      120

tcgcgacagt gagctgaaag ccgcgtcgca actgctcttt aacaatttat cagacaatct      180

gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga cgaaaaatga ataccaagtc      240

tcaagagtga acacgtaatt cattacgaag tttaattctt tgagcatcaa actttt          296


<210>  80
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(cspD)

<400>  80
cggttatcgg cagaacgccc tgccgctgac aagagcttat ttgcctgacc aatctactcc       60

acatgataga taactatcat ctatcgttgg catcagcgac atctgtcaca ttcctgtcaa      120

tagcgttaac tgcttcaaat ttttgattca tacctgcccc ctgtcccctg tcaaatgctt      180

gacggctcgc cctaattctc taaattgtat ttctagagtt ggcgaggttt tgaacagccc      240

cctctctgac cccggtttat tccatcttac ttgtataaga tttgcgaagg atgtcgaagc      300


<210>  81
<211>  299
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(299)
<223>  burden sensing promoter p(katE)

<400>  81
taaatccatc tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat       60

aatctggcgg ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt      120

aaaaatcaga aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta      180

tttcataaca ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt      240

agagagtttt ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttca       299


<210>  82
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(xthA)

<400>  82
caaaccatat gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa       60

aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt gtgatcattg      120

acgaaattta ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa agcgggcatt      180

ttttgcgcca tcgttgacat cattaacaat catcgatcaa atcacttaac aacaggcggt      240

aagcaacgcg aaattctgct accatccacg cactctttat ctgaataaat ggcagcgact      300


<210>  83
<211>  301
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(301)
<223>  burden sensing promoter p(uspE)

<400>  83
taatatctgg gcctgacggt agaaaccatc agccgtctgc tgggtcgctt ccagaaaagc       60

ggtatgctgg cagtcaaagg taaatacatc accatcgaaa ataacgatgc gctggcccag      120

cttgctggtc atacgcgtaa cgttgcctga tttttccgca taactcacta tccttctgtc      180

atatcattaa atttttctga tttattgatc tggcagaagg ttcatcactg tttcattcac      240

cagatatggg ttaatctttt aattacaaac tgcgttgaca gttgttgtaa ggagaccctg      300

t                                                                      301


<210>  84
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(gadB)

<400>  84
taattttata aatgcgttca aaataataat caagtactaa tagtgatatt ttaaggtctg       60

atttttacgt gataattcag gaggcacaga atgcgcataa aaataacagc ataaaacacc      120

ttaccaccac ccaagaattt catattgtat tgtttttcaa tgaaaaaata ttattcgcgt      180

aatatctcac gataaataac attaggattt tgttatttaa acacgagtcc tttgcacttg      240

cttactttat cgataaatcc tactttttta atgcgatcca atcattttaa ggagtttaaa      300


<210>  85
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(ahpC)

<400>  85
tagatcaggt gattgccctt tgtttatgag ggtgttgtaa tccatgtcgt tgttgcattt       60

gtaagggcaa cacctcagcc tgcaggcagg cactgaagat accaaagggt agttcagatt      120

acacggtcac ctggaaaggg ggccatttta ctttttatcg ccgctggcgg tgcaaagttc      180

acaaagttgt cttacgaagg ttgtaaggta aaacttatcg atttgataat ggaaacgcat      240

tagccgaatc ggcaaaaatt ggttacctta catctcatcg aaaacacgga ggaagtatag      300


<210>  86
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(katG)

<400>  86
gtgatcacaa attttaaaca gagcacaaaa tgctgcctcg aaatgagggc gggaaaataa       60

ggttatcagc cttgttttct ccctcattac ttgaaggata tgaagctaaa accctttttt      120

ataaagcatt tgtccgaatt cggacataat caaaaaagct taattaagat caatttgatc      180

tacatctctt taaccaacaa tatgtaagat ctcaactatc gcatccgtgg attaattcaa      240

ttataacttc tctctaacgc tgtgtatcgt aacggtaaca ctgtagaggg gagcacattg      300


<210>  87
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(grxA)

<400>  87
tagaatctgc acttctggcc gagaaaatgg ctcaggcagc gacagggagt cgcttaccga       60

cagcagcgcc accgccagta gcatcattcc gataaattcc agtatcaaca cgcctttagg      120

caatttaccg atcgcgcgca tacgcttccc tctgcaaagt gagccttcag tctaaaactt      180

ttcactgtat tgtgtttaac agttatagct tttagcaatt aatgcaacag gttaaaccta      240

ctttcagcga atacatttta gcgtgatcat tacaggcata aatctatgag gagagaaata      300


<210>  88
<211>  299
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(299)
<223>  burden sensing promoter p(oxyS)

<400>  88
taactgatcc accagcagca ttcccgcctg ggtgaacaac actttacggc tggtccgctc       60

cagcaacatc acgcccagct catcttccag cttacgaatt tgcccgctaa gcgtcggctg      120

gctaacgtgg caggaatctg ccgcacgccg aaaatggcgg tgttcagcca atgccaccag      180

gtactcaaga tcacgaatat tcattatcca tcctccatcg ccacgatagt tcatggcgat      240

aggtagaata gcaatgaacg attatcccta tcaagcattc tgactgataa ttgctcaca       299


<210>  89
<211>  300
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(300)
<223>  burden sensing promoter p(poxB)

<400>  89
taggttgtcg ctgcctgccg tgcgggtgtt tgcggctgct gtaagacaaa agtggtttcc       60

ggtgaatata cggtgagcag cacaatgacg ctgaccgacg ccgaaatcgc tgaaggttac      120

gtactggcct gctcctgcca tccgcagggg gatttggttc tcgcataatc gccttatgcc      180

cgatgatatt cctttcatcg ggctatttaa ccgttagtgc ctcctttctc tcccatccct      240

tccccctccg tcagatgaac taaacttgtt accgttatca cattcaggag atggagaacc      300


<210>  90
<211>  299
<212>  DNA
<213>  E. coli


<220>
<221>  promoter
<222>  (1)..(299)
<223>  burden sensing promoter p(trxC)

<400>  90
tagcgggttg tgggtacgtt tatcagaatc atcatcactg cggacataca tcactttgac       60

cttgccgctt ttacctttca tttcatcgtt catgcttttc tccaccagcg ctgcgcgaag      120

cgcgcagatt acccgaagtc cgcgcggttc gccatgattt cgtaccaaag cctgcgacta      180

tcatacctat tgaataaaac agattgttgt ctggaacaat gtccccgata atatgtaaca      240

tattagaaac ataccggcgt cgttgccgat aagtctcctt actcatcccg aggttagtt       299


<210>  91
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pKAR2

<400>  91
gtgggagtca atcaaatccc aatcgacgct attactggac aagtcaactt tattcgcaaa       60

acaagtcctg taatcatcac caaaagtctc aatgaatgct tcttcattaa gatactttga      120

ccattccata ggcggaaacg tgtttttatc caacactctg tcactcttgg accgtggctt      180

tggcgccctt tcattttgtc ttacataaaa gtatctcagc agcattattg cttctttcct      240

caagatcccc ggtatactct cgtaccctgc cgcactattg ttcttgggca ctaatgtaca      300

cgtatcatga tttactgaca agacagtacc gttgcctcca aatctctcgt tgccacaacc      360

gaacaccacc tttccaatgt ctaattgctt gagagcagat gcacacatta tacacggttc      420

tacagtaaca tatagagtaa tgtctttgaa cacgtcaaca actcctcggg agcccaacat      480

cgccttgatc tgatcgatcc ccatgaactc agcatgtgct actccagtta atgacttgtt      540

cgtatcgttc atgccataag ccatcacctg gccagttggc gtatgtacaa agatgcaagc      600

taccggtgtc tcatcgtggt caagagcgta tctagccaaa cggacagctg tcctcatatg      660

tttaatatgc tgcatagtgt gagtcctcta gtttttaccg cagccaccag ccgcttctcg      720

agcaaagtgt agatcccatt aggactcatc attcatctaa ttttgctatg ttagctgcaa      780

ctttctattt taatagaacc ttctggaaat ttcacccggc gcggcacccg aggaactgga      840

cagcgtgtcg aaaaagttgc ttttttatat aaaggacacg aaaagggttc tctggaagat      900

ataaatatgg ctatgtaatt ctaaagatta acgtgttact gttttacttt tttaaagtcc      960

ccaagagtag tctcaaggga aaaagcgtat caaacatacc                           1000


<210>  92
<211>  1000
<212>  DNA
<213>  S. cerevisiea


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pPDI1

<400>  92
caggtcgtca gatgtgacgt tctcatcgtc tagcaaatca tcgggaatct tggacttcat       60

ttgctccatg gagaaagtat gatctccatg caagttaaca gaacatatac ggaaattggt      120

accacccagg tcggcggcta gtaaaacacc gcgctccgtc ccgttgggtg acccggtgac      180

gaacgccgga atcataggaa gacctttgtc cgaggccaat gtgtggcctt cctttggtgg      240

agctagacct ttttccattt gttcgatgaa gtaagcagtt aattcgtcca gcttctcggg      300

ggtaacctcg aaatcgtcgc agatctggtc cacggcctgg atgaccgctc tctcagtggc      360

tttgtgtaag tcgtcgaatg acatcttgtg tatgatagag ttgtattagt ggtggtgttg      420

tggtttacgg aagctgagtt gggcactgat gggggggttg ggggttctgt tgatagcgga      480

tatttatatg ggcgcgtttt cattagtgcc caccgtttga gcgtggtgtg acaccacgcc      540

caagataggg ggccccctga aaggggttga ccgtccgtcg gcacaccact tataatgcgg      600

ggtgcaagcg ccgcgtctaa aatttttttt ttttccattt ttgtcgttat tgttatttcc      660

cgttttttgt tttttttgat tttttcggag cgacaaacct ttcgaaacac gtgtcctgaa      720

aattatcctg ggctgcacgt gataatatgt taccctgtcg ggcggcgcct ctttttccct      780

tttctctcac tagtctcttt ttccaatttg ccaccgtgta gcattttgtt gtgctgttac      840

aaccacaaca aaacgaaaaa cccgtatgga catacatata tatatatata tatatatata      900

ttttgttacg cgtgcatttt cttgttgcaa gcagcatgtc taattggtaa ttttaaagct      960

gccaagctct acataaagaa aaacatacat ctatcccgtt                           1000


<210>  93
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pSSA1

<400>  93
cactcatatc gggggtctta cttcccatca taaacacgga ccaaagaatt aaattcatca       60

ttcacataag cgaaaaaaag taatcattat tagttaactt ccctttacat gtaaccatag      120

gaaaaaaaaa aaagaaagaa aaacaacgat ctataaagta aactaaatcg gaacataccc      180

ttcaatgtat gacttgtcag ccaaagaagc gtttaattgt ttcaaagttt caatcttgga      240

gaaatcggtg gatgccatta tgtgtgtata tattcggttg gactcttcag tgctgaaagg      300

tagaaagcat gaggtaaaaa gataaagagt agaaggaaaa aaaaaagaac caactaaata      360

tagtgggtag ataaccttaa actatttatt tagaggcgac aatgtgaaaa attttttctt      420

caaaggctcg gttgtcgaca aattgttacg ttgtgctttg atttctaaag cgcttcttca      480

cctgcaggtt ctgagcccta agaaaaaaaa tttccttggt tgaaaatggc ggaaaaaaaa      540

aattcagaaa aagaaataaa gcacgtgtgc gcggtgtgtg gatgatggtt tcatcattgt      600

caacggcatt ttcgttcttg tggattgttg taaactttcc agaacattct agaaagaaag      660

cacacggaac gtttagaagc tgtcatttgc gttttttctc cagattttag ttgagaaagt      720

aattaaatta ttcttctttt tccagaacgt tccatcggcg gcaaaaggga gagaaagaac      780

ccaaaaagaa ggggggccat ttagattagc tgatcgtttc gaggacttca aggttatata      840

aggggtggat tgatgtatct tcgagaaggg attgagttgt agtttcgttt cccaattctt      900

acttaagttg ttttattttc tctatttgta agataagcac atcaaaagaa aagtaatcaa      960

gtattacaag aaacaaaaat tcaagtaaat aacagataat                           1000


<210>  94
<211>  1000
<212>  DNA
<213>  S. cerevisiea


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pFPR2

<400>  94
ctgatctttt cacgcagtta ggtaaggaaa atcgtggcca aataaatttc attgcattaa       60

actctacaat gttcccacac cacgttagat tcctaaatat gagagaacag ttcccattat      120

ttgctatcca taatatgatc aataatctga aatatggttt accacaacta ccagaagaag      180

agtacgcgaa attagaaaaa ccacaaccac tagacagaga tatgatcgtt cagttggtaa      240

aagattaccg tgaaggtact gccaagccaa ttgttaagtc agaagagatt ccaaaagaac      300

aaaagtccaa tgtttataaa atagttggga agacacatga cgacattgtt catgatgatg      360

acaaggatgt ccttgtcaaa tattacgcga catggtgtat tcatagtaaa aggtttgcgc      420

ctatttacga agaaattgca aatgtcttag catctgatga atctgttcgc gataaaatct      480

tgatcgccga agtagattca ggggcaaatg atatcttaag ttttcctgtg acaggatatc      540

caaccattgc tttgtatcct gccggaaata actctaagcc tattatcttc aataaaatta      600

gaaatttgga agatgttttc gaatttatca aggaatcagg tacacatcac attgacggcc      660

aggcaattta tgataaattg caccaggcca aggattctga agtgtctact gaagataccg      720

tacatgatga attataatca ataaataaag catatataat gcacattttt aacatctgat      780

tactcgcatc gtttctggaa gaaaatagct aatattcgtt atttatggca tcacgattat      840

tctcaccagt tacccgttta tgctcttgaa gagatttagc attactgcca gcgcatcttc      900

aaatacaggt ttatatgaga cccattacta taaccctaag aagagaaaaa ggagtgcttt      960

cgttttcaat cacattctag tttacagtaa ttgagtctcg                           1000


<210>  95
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pRPL3

<400>  95
cctttcctgc gggatagctt ttgcttcagg ttcttctgag gttctgaaaa tcaatatatt       60

tttccgacaa tttctcacta tgcctctatc ccccttaaaa agaaacactt tctaccctct      120

tttctaaact ctgcacacta gaccgagaca gcaaatcgga gcccgagaga aaatccggag      180

gacggaaaaa ctagaagtaa tcttatgcgg ggagcatgca ccagaacgcc cagaaaacgt      240

gaatgacact acatactttg atacggatgt tggtattttt ccggcttttt ctattttatc      300

gatgccgtgt ctgctgccat tacgtacagg taggcaaagg caaacaagaa tagcactttt      360

tcacttgctg cttttttttg tgaatgaagt ttatcgtata ttctgttagt gccgctcgta      420

tcagccactt acgttatcat tctaaagtaa gtatacgaaa tgtgcaaacc ttagtagacg      480

ttcaagaatt gctcgataat tgcgaacaaa ccagctgcta agccgggaag ttaagtcctc      540

aacacagaaa tgatgcaaac aatttattaa attcagtggt aatgcaacag caagaggaaa      600

ggtggaggga ttaacgcatt tcagacagct ttataggggg aaagaaagca ctcgcaaact      660

tgctgcctgt tcgcagtcat tggttgcaaa aactaaactc tactcacgca cactggaatg      720

aatggcaata ttctttttta ggttaaccgg ccggacagta atatagtaat cgttttgtac      780

gtttttcaag aagcgacgca caactgtttt ccattttttt tttttttttt tcagtgatca      840

tcgtccatga aaaaaatttt tcatttgtct ctttcgtgct tcctggatat ataaaatacg      900

atttatttag ttgtctttgt caatcctcat ctttctttac tcattatttc atttcggttt      960

tgtcatctct agaacaacac agttactaca acaatcaatc                           1000


<210>  96
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pRPL6A

<400>  96
attctcgtgc aagcagtttt gcgcgtactt tagctcctca tggcacttac aatggtttta       60

ttaccgtggt tgctgcggaa aacgttgcca agcatgttca aattaagatc tctttaactc      120

aaggtggtag actaaaacac atatacaaaa cgaaaagcca aaaagccaat aatgatggtg      180

ttgccgtatt tgatgaagag tgctcgttca aggcttctcc cgaagccaat ttggtactgg      240

gtgcaatttc ccatcaaaga ctatcgaggg acaaagatct tggtattgct caaatcaact      300

tgggtgaccc tcaaattcaa caagatggcc aaatttctgt aaaattagga gacggtcatc      360

tgattgtaaa gattaattac ggtaaagaca agaatggtca ggtacctccc gtgccagaag      420

ttcctcaaga atacacgcag taataaacag tgaaaactat tcttttatac gctcaataag      480

ggcacatttg gtgtgttaac aggtgtcttt tctgtgtttg ttataatttt gcgctattat      540

tatataacgt tatatttact ataatatatt acaacatata ttacaacata tttcaacgct      600

ctcctgttac tgcttaggga gaagaaagaa atgtgaagac tgctattaaa tccgtacatt      660

attaaaatgt atggatttgt tatatttttc ttcagttttt tccttaacca gattcgaaaa      720

gcggcccgcc tagaccattg cggttgcggc taaagcgttc tggagagggc ttctccgctg      780

ggcggacaac agaagcgaga gacacgtttc ccgtctggga ggactcaccg gatttgtcca      840

gtttcccact ggagtgtgct ttgttccttt tgcaaaattt cgttggtatg gaggttatcg      900

aatggaaggg ttacatgaat agattaaatg tactactcat taaagattga atatcagttt      960

tacattaggc gaaacaacaa agtaatagcc atccaacgaa                           1000


<210>  97
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  pRPL28

<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pRPL28

<400>  97
gggatagccc tccatgggaa tgtcaaattc tgagcacgaa agcacctgct gaggtgcatt       60

taattccgac aagtggtaac caaattgtat tgaaccgatt gaggctacta taattgcata      120

acataacgcc tttgtaattt tatgcttgtt ttgccttggc atgctaacat tggatagcaa      180

cgatgcacta gatagaatcc tattcatact atgctacgta cctgtttaac tcttctcatt      240

ttatccgttt tctttcttca ccgttcctct taagttcttt atttttttca taacagaatg      300

atcacccctt tcactttgcc gccaatataa tattaacaca caagaaataa gtatgaggta      360

gtttgctctc gaaaaaacca agtaatagtt caaaaaatga aaaaaagaaa acaaatacca      420

aattatgcca ccgttacctt acgtttcatg gttaatcatc gtttactgcc gcctatgagc      480

gtaagctaat gttataaaga aacaagctat aatattgtta aatatagttg atcaacagca      540

ttgtaatgat tacaagagac gaggtggaat gaaccttatg aaatgcgtat tatatataaa      600

ctgtaataag agctaagttg aattgaaatc tacgatactt gatgttgaca ttatagcact      660

agttcccagg aaaccctttc gaaaaacaca gcaaaaacaa gagtactgta accaatgtaa      720

catctgtaca ccagggaccc acacattacc aaaatcaaaa ttatttttct aatgcctgtt      780

atttttccta tttttcctct ggcgcgtgaa tagcccgcag agacgcaaac aattttcctc      840

gcagtttttc gcttgtttaa tgcgtatttt cccagatagg ttcaaacctt tcatctgtat      900

cccgtatatt taagatggcg tttgctttct ccgttgattt ttttccttct tagtgatttt      960

tttgcattaa atcccagaac aatcatccaa ctaatcaaga                           1000


<210>  98
<211>  580
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(580)
<223>  burden sensing promoter pOGG1

<400>  98
tgcaatcagg ttcttcccaa tcatccgaat cagagagcaa atctaggtct tcttttgaaa       60

gtatggaagg atgcttcaat tgtgcttgaa taaagttatt agacctgaaa ccacgactac      120

tcatagaaaa cgatagtttg gcgtgcgata cgtttttgag taaaagaaaa ctgttgaaac      180

tacagataaa tggccttctt ggtataatat gattcaatgt tgatcttatc cactttggca      240

tgtaaaattg attgatacaa gtaagctaat cgctcaatgg ataatattac aaataaaaat      300

atcgaggcaa taccagtatt tcaatgtctg gccaaataaa tagtttagta cagcaatttg      360

attaacgtta aagttactct cctctttgaa taatgtcgtg gttaaaaagt aaagtatttg      420

tttatacatt aagtgaagaa aattaagacg cgcctttctt taatgtaacg ccaaaaattt      480

tcaagagatg agctgaacag catcacaaaa ctactatttc cagcggaaga aggcatttga      540

agcgtcctga ttcataattg cgattttatt tatcaaccag                            580


<210>  99
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pRAD51

<400>  99
ccgcaataaa gggcttcccg gacttggaat taatacctgc taccaccggc cccacaaagt       60

atggaccgaa tcttctttca taaagtgaac ttgacactaa ttgggtaaac gtttcaggct      120

caatggccct ttcttccttc aacttatata ggttcgtctt gtagcggaac atctcgttca      180

gagtagtcac gtcggtagcc agaccggtaa tccctaagaa cacatgccca tagtggaaga      240

ttttttcaaa cttgttcgac acacctagtg actggcttcc gagacgcaaa tcacaggcaa      300

tggccacaca gtctttacct gtcatcgcaa cgacgatacc accgttaata gaacttggat      360

ccgacatttt ttttttatgc tttattcact gttcaatatt ttcaccacaa ttcgcaagaa      420

acgcactcta cttcgaaact acggttcaaa cttacttagc agcttcccga tttaattggc      480

ctttctacta tgccataaac tctttcttcc tctcttttca tcgcccctgc atttgcactt      540

ttttgccacc ggcagtgcca tccggtcaca tgactacacc acgttaatag cgatctggct      600

tatcattgtc acagagtaaa ttaaaatgga cggtaaatgt tggaaatgca ccactaccgt      660

tcttcaacca atctagttta gctatcctgc aacaggtggc cttcttgagc attccctgag      720

cattccaacc ggttgtatca gtgttttatc accgtctcac catatcccac gactaggcca      780

cacttcgtta ccctatgcta cgcgtcattt ccgctatttc tgtcctggtt tgtttacagt      840

acgcgtggtg ggaccataaa ggggaatagt ggggactgga gaaaaaattt tctcagttac      900

ttcttctatc ttccgtagtt tccatatact agtagttgag tgtagcgaca aagagcagac      960

gtagttattt gttaaaggcc tactaatttg ttatcgtcat                           1000


<210>  100
<211>  1000
<212>  DNA
<213>  S. cerevisiae


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  burden sensing promoter pRAD54

<400>  100
tgcgctacgg ttcctgccgc tcgtgggaac cccacgcaaa acatattatt cgcttctctc       60

tgctgacaac tccggtttac gttataccgt attaggatca ctataagggt tccttcggga      120

ggagggggga ggggaagaat gtacatcgtc ataaggcctt tatggtgtga agtgggtttt      180

gcgtggaaaa ttcgttttca atgatataga gcccacgcat atacgtacat actagtggcc      240

aaaagcgtgg ggtgggcgga caaagctaca ctggtaaaat acaggattct atgaacaata      300

acaacaacca gctcacgttg ctgaacagcc gaggtcagcc gatgcaaccg aggtttccaa      360

agtagcattt ctgtgctagc tatgtctgta ggtttacatt taatggtgcg tggttccagc      420

ttcatgtgct tgcatgtgat gtcctgcaga tggtaagaag attctgaaag ccgcgctagg      480

agaaaaatat tctgctcgaa gatctgtcct cttaagtaga aagcgtgaaa ttgttgcgtt      540

cttgcattac tactcaacgc gtacgcaaat gcgtctactg cacctgcatg ataaagctta      600

tgtatcaaaa atttaacatc ttgaaaatac acaagtggtg caaagatgtg tcacgttctg      660

gacctgagtg gtgccatgta tgctatttaa catgcaaagg ggaagaccct tccgccttac      720

tgcaataata aaaagtattt tacgcgttac ccaatatagc aaagtttcgc gcaaaaaaaa      780

aaataaaaaa caattacaaa caaaaagaaa aaaaaggaaa taatagaaga tctaactgaa      840

gcgaaggcca aaactcttct cacttgacgt aatagccgat acaaaatcta gagcagcaac      900

ttttctcttt cttcactaaa gctgctacga aagtatagaa aaatcaaacg ctcagaactt      960

agctctattt caaggtacca tatatatttc cttataactg                           1000


<210>  101
<211>  5776
<212>  DNA
<213>  synthetic


<220>
<221>  plasmid
<222>  (1)..(5776)
<223>  pEG34

<220>
<221>  CDS
<222>  (463)..(1758)
<223>  hGH-glycine linker-GFP

<400>  101
atcgatgcat gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat       60

gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg      120

tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt      180

tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg      240

ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg      300

aggtcgacgg tatcgataag cttgatatca gctgaattca ttaaagagga gaaaggtacc      360

atgggtctgg gcaagaaaaa gaaactgctg ctgctgttac tgagcagcgc agttgcagca      420

agctttatga gcctgaccat tagcctgcct ggtgttcagg ca ttt ccg acc att         474
                                               Phe Pro Thr Ile            
                                               1                          

ccg ctg agc cgt ctg ttt gat aat gca atg ctg cgt gca cat cgt ctg        522
Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg Ala His Arg Leu           
5                   10                  15                  20            

cat cag ctg gca ttt gat acc tat caa gaa ttt gaa gaa gcg tat atc        570
His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr Ile           
                25                  30                  35                

ccg aaa gag cag aaa tat agc ttc ctg cag aat ccg cag acc agc ctg        618
Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser Leu           
            40                  45                  50                    

tgt ttt agc gaa agc att ccg aca ccg agc aat cgt gaa gaa acc cag        666
Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr Gln           
        55                  60                  65                        

cag aaa agc aat ctg gaa ctg ctg cgt att agt ctg ctg ctg att cag        714
Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile Gln           
    70                  75                  80                            

agc tgg ctg gaa ccg gtg cag ttt ctg cgt agc gtt ttt gca aat agc        762
Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe Ala Asn Ser           
85                  90                  95                  100           

ctg gtt tat ggt gca agc gat agc aat gtt tat gat ctg ctg aaa gat        810
Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys Asp           
                105                 110                 115               

ctg gaa gaa ggt att cag acc ctg atg ggt cgt ctg gaa gat ggt tca        858
Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu Glu Asp Gly Ser           
            120                 125                 130                   

ccg cgt acc ggt cag atc ttt aaa cag acc tat agc aaa ttc gat acc        906
Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr           
        135                 140                 145                       

aac agc cat aat gat gat gcc ctg ctg aaa aac tat ggt ctg ctg tat        954
Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr           
    150                 155                 160                           

tgt ttc cgc aaa gat atg gat aaa gtg gaa acc ttt ctg cgc att gtt       1002
Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe Leu Arg Ile Val           
165                 170                 175                 180           

cag tgt cgt agc gtt gaa ggt agc tgt ggt ttt ggt ggc atg cgt aaa       1050
Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe Gly Gly Met Arg Lys           
                185                 190                 195               

ggc gaa gag ctg ttc act ggt ttc gtc act att ctg gtg gaa ctg gat       1098
Gly Glu Glu Leu Phe Thr Gly Phe Val Thr Ile Leu Val Glu Leu Asp           
            200                 205                 210                   

ggt gat gtc aac ggt cat aag ttt tcc gtg cgt ggc gag ggt gaa ggt       1146
Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu Gly Glu Gly           
        215                 220                 225                       

gac gca act aat ggt aaa ctg acg ctg aag ttc atc tgt act act ggt       1194
Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly           
    230                 235                 240                           

aaa ctg ccg gta cct tgg ccg act ctg gta acg acg ctg act tat ggt       1242
Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly           
245                 250                 255                 260           

gtt cag tgc ttt gct cgt tat ccg gac cac atg aag cag cat gac ttc       1290
Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe           
                265                 270                 275               

ttc aag tcc gcc atg ccg gaa ggc tat gtg cag gaa cgc acg att tcc       1338
Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser           
            280                 285                 290                   

ttt aag gat gac ggc acg tac aaa acg cgt gcg gaa gtg aaa ttt gaa       1386
Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu           
        295                 300                 305                       

ggc gat acc ctg gta aac cgc att gag ctg aaa ggc att gac ttt aaa       1434
Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys           
    310                 315                 320                           

gaa gac ggc aat atc ctg ggc cat aag ctg gaa tac aat ttt aac agc       1482
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Phe Asn Ser           
325                 330                 335                 340           

cac aat gtt tac atc acc gcc gat aaa caa aaa aat ggc att aaa gcg       1530
His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala           
                345                 350                 355               

aat ttt aaa att cgc cac aac gtg gag gat ggc agc gtg cag ctg gct       1578
Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val Gln Leu Ala           
            360                 365                 370                   

gat cac tac cag caa aac act cca atc ggt gat ggt cct gtt ctg ctg       1626
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu           
        375                 380                 385                       

cca gac aat cac tat ctg agc acg caa agc gtt ctg tct aaa gat ccg       1674
Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser Lys Asp Pro           
    390                 395                 400                           

aac gag aaa cgc gat cac atg gtt ctg ctg gag ttc gta acc gca gcg       1722
Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala           
405                 410                 415                 420           

ggc atc acg cat ggt atg gat gaa ctg tac aaa taa gtcgacctgc            1768
Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys                               
                425                 430                                   

aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta     1828

aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg     1888

tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg     1948

ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg     2008

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca     2068

aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga     2128

cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt     2188

tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct     2248

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat     2308

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc     2368

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cagcaaacta     2428

ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg     2488

gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat     2548

aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt     2608

aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga     2668

aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa     2728

gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc     2788

gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc     2848

tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa     2908

tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact     2968

tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt     3028

gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa     3088

ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt     3148

aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac     3208

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     3268

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     3328

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     3388

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     3448

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     3508

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gggcatttga     3568

gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata     3628

agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt     3688

ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc     3748

aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt     3808

cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca     3868

gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga     3928

agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg     3988

ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt     4048

aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac     4108

tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac     4168

tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca     4228

tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg     4288

tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg     4348

actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc     4408

cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa     4468

atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat     4528

caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag     4588

gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt     4648

cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg     4708

gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc     4768

aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg     4828

atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca     4888

gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt     4948

cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc     5008

caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc     5068

cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca     5128

ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct     5188

gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct     5248

gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt     5308

tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca     5368

tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt     5428

catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc     5488

agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg     5548

cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc     5608

atcttattaa tcagataaaa tatttgctca tgagcccgaa gtggcgagcc cgatcttccc     5668

catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg     5728

ccacgatgcg tccggcgtag aggatctgct catgtttgac agcttatc                  5776


<210>  102
<211>  431
<212>  PRT
<213>  synthetic

<400>  102

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe Gly 
            180                 185                 190         


Gly Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Phe Val Thr Ile Leu 
        195                 200                 205             


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
    210                 215                 220                 


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
225                 230                 235                 240 


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
                245                 250                 255     


Leu Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
            260                 265                 270         


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
        275                 280                 285             


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
    290                 295                 300                 


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
305                 310                 315                 320 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
                325                 330                 335     


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
            340                 345                 350         


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
        355                 360                 365             


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
    370                 375                 380                 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu 
385                 390                 395                 400 


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
                405                 410                 415     


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
            420                 425                 430     


<210>  103
<211>  660
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(660)
<223>  Chloramphenicol_acetyltransferase

<400>  103
atg gag aaa aaa atc act gga tat acc acc gtt gat ata tcc caa tgg         48
Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp           
1               5                   10                  15                

cat cgt aaa gaa cat ttt gag gca ttt cag tca gtt gct caa tgt acc         96
His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr           
            20                  25                  30                    

tat aac cag acc gtt cag ctg gat att acg gcc ttt tta aag acc gta        144
Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val           
        35                  40                  45                        

aag aaa aat aag cac aag ttt tat ccg gcc ttt att cac att ctt gcc        192
Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala           
    50                  55                  60                            

cgc ctg atg aat gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt        240
Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly           
65                  70                  75                  80            

gag ctg gtg ata tgg gat agt gtt cac cct tgt tac acc gtt ttc cat        288
Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His           
                85                  90                  95                

gag caa act gaa acg ttt tca tcg ctc tgg agt gaa tac cac gac gat        336
Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp           
            100                 105                 110                   

ttc cgg cag ttt cta cac ata tat tcg caa gat gtg gcg tgt tac ggt        384
Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly           
        115                 120                 125                       

gaa aac ctg gcc tat ttc cct aaa ggg ttt att gag aat atg ttt ttc        432
Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe           
    130                 135                 140                           

gtc tca gcc aat ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg        480
Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val           
145                 150                 155                 160           

gcc aat atg gac aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat        528
Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr           
                165                 170                 175               

tat acg caa ggc gac aag gtg ctg atg ccg ctg gcg att cag gtt cat        576
Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His           
            180                 185                 190                   

cat gcc gtc tgt gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta        624
His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu           
        195                 200                 205                       

caa cag tac tgc gat gag tgg cag ggc ggg gcg taa                        660
Gln Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala                               
    210                 215                                               


<210>  104
<211>  219
<212>  PRT
<213>  synthetic

<400>  104

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala 
    210                 215                 


<210>  105
<211>  4347
<212>  DNA
<213>  synthetic


<220>
<221>  plasmid
<222>  (1)..(4347)
<223>  pEG0

<400>  105
atcgatgcat gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat       60

gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg      120

tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt      180

tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg      240

ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg      300

aggtcgacgg tatcgataag cttgatatcg aattcctgca ggcatgcaag cttggctgtt      360

ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc      420

tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga      480

actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag      540

ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt      600

atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg      660

aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg      720

catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt      780

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata      840

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct      900

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa      960

agtaaaagat gctgaagatc agttgggtgc agcaaactat taactggcga actacttact     1020

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     1080

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     1140

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     1200

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     1260

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     1320

attgatttac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag     1380

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt     1440

tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt     1500

ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg     1560

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt     1620

taatagtgga ctcttgttcc aaacttgaac aacactcaac cctatctcgg gctattcttt     1680

tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca     1740

aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaaagg atctaggtga     1800

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     1860

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     1920

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     1980

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     2040

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     2100

acctcgctct gctaatcctg ttaccagtgg ggcatttgag aagcacacgg tcacactgct     2160

tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg     2220

ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta     2280

tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt     2340

acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat     2400

ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc     2460

cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca     2520

cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct     2580

caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat     2640

atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt     2700

cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac     2760

cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa     2820

taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat     2880

ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt     2940

ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt     3000

tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta     3060

tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa     3120

agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt     3180

gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac     3240

tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt     3300

ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag     3360

cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc     3420

ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat     3480

atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga     3540

aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga     3600

agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac     3660

gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg     3720

tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc     3780

attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc     3840

agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt     3900

atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc     3960

agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa     4020

ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt     4080

tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc     4140

aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat     4200

atttgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat     4260

aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga     4320

ggatctgctc atgtttgaca gcttatc                                         4347


<210>  106
<211>  6076
<212>  DNA
<213>  synthetic


<220>
<221>  plasmid
<222>  (1)..(6076)
<223>  pENDU5CAM

<220>
<221>  CDS
<222>  (108)..(911)
<223>  ompA-lysostaphin

<400>  106
gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat       60

tcccctctag aaataatttt gtttaacttt aagaaggaga tatacat atg aaa aag        116
                                                    Met Lys Lys           
                                                    1                     

aca gct atc gcg att gca gtg gca ctg gct ggt ttc gct acc gta gcg        164
Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala Thr Val Ala           
    5                   10                  15                            

cag gct gca gca acc cat gaa cat agc gca cag tgg ctg aat aat tac        212
Gln Ala Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr           
20                  25                  30                  35            

aaa aaa ggt tat ggc tat ggt ccg tat ccg ctg ggt att aat ggt ggt        260
Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly           
                40                  45                  50                

atg cat tat ggt gtg gat ttc ttt atg aat att ggc aca ccg gtt aaa        308
Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys           
            55                  60                  65                    

gca att agc agc ggt aaa att gtt gaa gca ggt tgg agc aat tat ggt        356
Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly           
        70                  75                  80                        

ggt ggt aat cag att ggc ctg att gaa aat gat ggt gtt cat cgt cag        404
Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln           
    85                  90                  95                            

tgg tat atg cat ctg agc aaa tac aat gtg aaa gtg ggc gat tat gtt        452
Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val           
100                 105                 110                 115           

aaa gcc ggt cag att att ggt tgg agc ggt agc acc ggt tat agt acc        500
Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr           
                120                 125                 130               

gca ccg cat ctg cat ttt cag cgt atg gtt aat agc ttt agc aat agc        548
Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser           
            135                 140                 145                   

acc gca cag gat ccg atg ccg ttt ctg aaa agc gca ggt tat ggt aaa        596
Thr Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys           
        150                 155                 160                       

gca ggc ggt aca gtt acc ccg aca ccg aat acc ggt tgg aaa acc aac        644
Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn           
    165                 170                 175                           

aaa tat ggc acc ctg tat aaa agc gaa agc gca agc ttt acc ccg aat        692
Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn           
180                 185                 190                 195           

aca gat att atc acc cgt acc acc ggt ccg ttt cgt agc atg ccg cag        740
Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln           
                200                 205                 210               

agc ggt gtt ctg aaa gca ggt cag acc att cat tat gat gaa gtg atg        788
Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met           
            215                 220                 225                   

aaa cag gat ggt cat gtt tgg gtt ggt tat acc ggt aat agc ggt cag        836
Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln           
        230                 235                 240                       

cgt att tat ctg ccg gtt cgt acc tgg aat aaa agc acc aat aca ctg        884
Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu           
    245                 250                 255                           

ggt gtt ctg tgg ggc acc atc aaa taa tgagatccgg ctgctaacaa              931
Gly Val Leu Trp Gly Thr Ile Lys                                           
260                 265                                                   

agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct      991

tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt     1051

ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc     1111

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc     1171

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg     1231

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca     1291

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc     1351

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct     1411

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa     1471

caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt     1531

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat     1591

ccgctcaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt     1651

gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa     1711

tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg     1771

gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg     1831

gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt     1891

caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt     1951

attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt     2011

gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag     2071

cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct     2131

ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag     2191

caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg     2251

tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact     2311

caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt     2371

gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga     2431

caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa     2491

gtgcgtcatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     2551

accccgtaga aaagattgtt catgaccaaa atcccttaac gtgatcaaag gatcttcttg     2611

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     2671

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     2731

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa     2791

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     2851

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     2911

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     2971

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     3031

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     3091

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     3151

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     3211

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt     3271

atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg     3331

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg     3391

gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt     3451

acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact     3511

gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc     3571

tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga     3631

ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt     3691

cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca     3751

gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt     3811

tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga     3871

aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac     3931

gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg     3991

gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc     4051

ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt     4111

acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc     4171

agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac     4231

cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg     4291

ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga     4351

aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg     4411

cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta     4471

cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc     4531

accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct     4591

aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     4651

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta     4711

ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc     4771

accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga     4831

aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg     4891

tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt     4951

gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc     5011

agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct     5071

atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc     5131

gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga     5191

tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc     5251

tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg     5311

gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga     5371

ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg     5431

ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc     5491

agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt     5551

gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt     5611

ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg     5671

gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc     5731

tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg     5791

atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag     5851

gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc     5911

cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg     5971

gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt     6031

ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcga                     6076


<210>  107
<211>  267
<212>  PRT
<213>  synthetic

<400>  107

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Ala Ala Thr His Glu His Ser Ala Gln Trp Leu 
            20                  25                  30          


Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile 
        35                  40                  45              


Asn Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr 
    50                  55                  60                  


Pro Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser 
65                  70                  75                  80  


Asn Tyr Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val 
                85                  90                  95      


His Arg Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly 
            100                 105                 110         


Asp Tyr Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly 
        115                 120                 125             


Tyr Ser Thr Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe 
    130                 135                 140                 


Ser Asn Ser Thr Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly 
145                 150                 155                 160 


Tyr Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp 
                165                 170                 175     


Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe 
            180                 185                 190         


Thr Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser 
        195                 200                 205             


Met Pro Gln Ser Gly Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp 
    210                 215                 220                 


Glu Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn 
225                 230                 235                 240 


Ser Gly Gln Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr 
                245                 250                 255     


Asn Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 
            260                 265         


<210>  108
<211>  8593
<212>  DNA
<213>  synthetic


<220>
<221>  plasmid
<222>  (1)..(8593)
<223>  pMevT

<220>
<221>  CDS
<222>  (380)..(1564)
<223>  Acetyl-CoA_acetyltransferase

<220>
<221>  CDS
<222>  (1578)..(3053)
<223>  3-hydroxy-3-methylglutaryl-coenzyme_A_synthase

<220>
<221>  CDS
<222>  (3067)..(4575)
<223>  N-Terminally_Truncated_3-Hydroxy-3-Methylglutaryl_Coenzyme_A_Redu
       ctase

<400>  108
atcgatgcat gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat       60

gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg      120

tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt      180

tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg      240

ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg      300

aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggat cctctagagt      360

cgactaggag gaatataaa atg aaa aat tgt gtc atc gtc agt gcg gta cgt       412
                     Met Lys Asn Cys Val Ile Val Ser Ala Val Arg          
                     1               5                   10               

act gct atc ggt agt ttt aac ggt tca ctc gct tcc acc agc gcc atc        460
Thr Ala Ile Gly Ser Phe Asn Gly Ser Leu Ala Ser Thr Ser Ala Ile           
            15                  20                  25                    

gac ctg ggg gcg aca gta att aaa gcc gcc att gaa cgt gca aaa atc        508
Asp Leu Gly Ala Thr Val Ile Lys Ala Ala Ile Glu Arg Ala Lys Ile           
        30                  35                  40                        

gat tca caa cac gtt gat gaa gtg att atg ggt aac gtg tta caa gcc        556
Asp Ser Gln His Val Asp Glu Val Ile Met Gly Asn Val Leu Gln Ala           
    45                  50                  55                            

ggg ctg ggg caa aat ccg gcg cgt cag gca ctg tta aaa agc ggg ctg        604
Gly Leu Gly Gln Asn Pro Ala Arg Gln Ala Leu Leu Lys Ser Gly Leu           
60                  65                  70                  75            

gca gaa acg gtg tgc gga ttc acg gtc aat aaa gta tgt ggt tcg ggt        652
Ala Glu Thr Val Cys Gly Phe Thr Val Asn Lys Val Cys Gly Ser Gly           
                80                  85                  90                

ctt aaa agt gtg gcg ctt gcc gcc cag gcc att cag gca ggt cag gcg        700
Leu Lys Ser Val Ala Leu Ala Ala Gln Ala Ile Gln Ala Gly Gln Ala           
            95                  100                 105                   

cag agc att gtg gcg ggg ggt atg gaa aat atg agt tta gcc ccc tac        748
Gln Ser Ile Val Ala Gly Gly Met Glu Asn Met Ser Leu Ala Pro Tyr           
        110                 115                 120                       

tta ctc gat gca aaa gca cgc tct ggt tat cgt ctt gga gac gga cag        796
Leu Leu Asp Ala Lys Ala Arg Ser Gly Tyr Arg Leu Gly Asp Gly Gln           
    125                 130                 135                           

gtt tat gac gta atc ctg cgc gat ggc ctg atg tgc gcc acc cat ggt        844
Val Tyr Asp Val Ile Leu Arg Asp Gly Leu Met Cys Ala Thr His Gly           
140                 145                 150                 155           

tat cat atg ggg att acc gcc gaa aac gtg gct aaa gag tac gga att        892
Tyr His Met Gly Ile Thr Ala Glu Asn Val Ala Lys Glu Tyr Gly Ile           
                160                 165                 170               

acc cgt gaa atg cag gat gaa ctg gcg cta cat tca cag cgt aaa gcg        940
Thr Arg Glu Met Gln Asp Glu Leu Ala Leu His Ser Gln Arg Lys Ala           
            175                 180                 185                   

gca gcc gca att gag tcc ggt gct ttt aca gcc gaa atc gtc ccg gta        988
Ala Ala Ala Ile Glu Ser Gly Ala Phe Thr Ala Glu Ile Val Pro Val           
        190                 195                 200                       

aat gtt gtc act cga aag aaa acc ttc gtc ttc agt caa gac gaa ttc       1036
Asn Val Val Thr Arg Lys Lys Thr Phe Val Phe Ser Gln Asp Glu Phe           
    205                 210                 215                           

ccg aaa gcg aat tca acg gct gaa gcg tta ggt gca ttg cgc ccg gcc       1084
Pro Lys Ala Asn Ser Thr Ala Glu Ala Leu Gly Ala Leu Arg Pro Ala           
220                 225                 230                 235           

ttc gat aaa gca gga aca gtc acc gct ggg aac gcg tct ggt att aac       1132
Phe Asp Lys Ala Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Ile Asn           
                240                 245                 250               

gac ggt gct gcc gct ctg gtg att atg gaa gaa tct gcg gcg ctg gca       1180
Asp Gly Ala Ala Ala Leu Val Ile Met Glu Glu Ser Ala Ala Leu Ala           
            255                 260                 265                   

gca ggc ctt acc ccc ctg gct cgc att aaa agt tat gcc agc ggt ggc       1228
Ala Gly Leu Thr Pro Leu Ala Arg Ile Lys Ser Tyr Ala Ser Gly Gly           
        270                 275                 280                       

gtg ccc ccc gca ttg atg ggt atg ggg cca gta cct gcc acg caa aaa       1276
Val Pro Pro Ala Leu Met Gly Met Gly Pro Val Pro Ala Thr Gln Lys           
    285                 290                 295                           

gcg tta caa ctg gcg ggg ctg caa ctg gcg gat att gat ctc att gag       1324
Ala Leu Gln Leu Ala Gly Leu Gln Leu Ala Asp Ile Asp Leu Ile Glu           
300                 305                 310                 315           

gct aat gaa gca ttt gct gca cag ttc ctt gcc gtt ggg aaa aac ctg       1372
Ala Asn Glu Ala Phe Ala Ala Gln Phe Leu Ala Val Gly Lys Asn Leu           
                320                 325                 330               

ggc ttt gat tct gag aaa gtg aat gtc aac ggc ggg gcc atc gcg ctc       1420
Gly Phe Asp Ser Glu Lys Val Asn Val Asn Gly Gly Ala Ile Ala Leu           
            335                 340                 345                   

ggg cat cct atc ggt gcc agt ggt gct cgt att ctg gtc aca cta tta       1468
Gly His Pro Ile Gly Ala Ser Gly Ala Arg Ile Leu Val Thr Leu Leu           
        350                 355                 360                       

cat gcc atg cag gca cgc gat aaa acg ctg ggg ctg gca aca ctg tgc       1516
His Ala Met Gln Ala Arg Asp Lys Thr Leu Gly Leu Ala Thr Leu Cys           
    365                 370                 375                           

att ggc ggc ggt cag gga att gcg atg gtg att gaa cgg ttg aat taa       1564
Ile Gly Gly Gly Gln Gly Ile Ala Met Val Ile Glu Arg Leu Asn               
380                 385                 390                               

ggaggacagc taa atg aaa ctc tca act aaa ctt tgt tgg tgt ggt att        1613
               Met Lys Leu Ser Thr Lys Leu Cys Trp Cys Gly Ile            
               395                 400                 405                

aaa gga aga ctt agg ccg caa aag caa caa caa tta cac aat aca aac       1661
Lys Gly Arg Leu Arg Pro Gln Lys Gln Gln Gln Leu His Asn Thr Asn           
            410                 415                 420                   

ttg caa atg act gaa cta aaa aaa caa aag acc gct gaa caa aaa acc       1709
Leu Gln Met Thr Glu Leu Lys Lys Gln Lys Thr Ala Glu Gln Lys Thr           
        425                 430                 435                       

aga cct caa aat gtc ggt att aaa ggt atc caa att tac atc cca act       1757
Arg Pro Gln Asn Val Gly Ile Lys Gly Ile Gln Ile Tyr Ile Pro Thr           
    440                 445                 450                           

caa tgt gtc aac caa tct gag cta gag aaa ttt gat ggc gtt tct caa       1805
Gln Cys Val Asn Gln Ser Glu Leu Glu Lys Phe Asp Gly Val Ser Gln           
455                 460                 465                 470           

ggt aaa tac aca att ggt ctg ggc caa acc aac atg tct ttt gtc aat       1853
Gly Lys Tyr Thr Ile Gly Leu Gly Gln Thr Asn Met Ser Phe Val Asn           
                475                 480                 485               

gac aga gaa gat atc tac tcg atg tcc cta act gtt ttg tct aag ttg       1901
Asp Arg Glu Asp Ile Tyr Ser Met Ser Leu Thr Val Leu Ser Lys Leu           
            490                 495                 500                   

atc aag agt tac aac atc gac acc aac aaa att ggt aga tta gaa gtc       1949
Ile Lys Ser Tyr Asn Ile Asp Thr Asn Lys Ile Gly Arg Leu Glu Val           
        505                 510                 515                       

ggt act gaa act ctg att gac aag tcc aag tct gtc aag tct gtc ttg       1997
Gly Thr Glu Thr Leu Ile Asp Lys Ser Lys Ser Val Lys Ser Val Leu           
    520                 525                 530                           

atg caa ttg ttt ggt gaa aac act gac gtc gaa ggt att gac acg ctt       2045
Met Gln Leu Phe Gly Glu Asn Thr Asp Val Glu Gly Ile Asp Thr Leu           
535                 540                 545                 550           

aat gcc tgt tac ggt ggt acc aac gcg ttg ttc aac tct ttg aac tgg       2093
Asn Ala Cys Tyr Gly Gly Thr Asn Ala Leu Phe Asn Ser Leu Asn Trp           
                555                 560                 565               

att gaa tct aac gca tgg gat ggt aga gac gcc att gta gtt tgc ggt       2141
Ile Glu Ser Asn Ala Trp Asp Gly Arg Asp Ala Ile Val Val Cys Gly           
            570                 575                 580                   

gat att gcc atc tac gat aag ggt gcc gca aga cca acc ggt ggt gcc       2189
Asp Ile Ala Ile Tyr Asp Lys Gly Ala Ala Arg Pro Thr Gly Gly Ala           
        585                 590                 595                       

ggt act gtt gct atg tgg atc ggt cct gat gct cca att gta ttt gac       2237
Gly Thr Val Ala Met Trp Ile Gly Pro Asp Ala Pro Ile Val Phe Asp           
    600                 605                 610                           

tct gta aga gct tct tac atg gaa cac gcc tac gat ttt tac aag cca       2285
Ser Val Arg Ala Ser Tyr Met Glu His Ala Tyr Asp Phe Tyr Lys Pro           
615                 620                 625                 630           

gat ttc acc agc gaa tat cct tac gtc gat ggt cat ttt tca tta act       2333
Asp Phe Thr Ser Glu Tyr Pro Tyr Val Asp Gly His Phe Ser Leu Thr           
                635                 640                 645               

tgt tac gtc aag gct ctt gat caa gtt tac aag agt tat tcc aag aag       2381
Cys Tyr Val Lys Ala Leu Asp Gln Val Tyr Lys Ser Tyr Ser Lys Lys           
            650                 655                 660                   

gct att tct aaa ggg ttg gtt agc gat ccc gct ggt tcg gat gct ttg       2429
Ala Ile Ser Lys Gly Leu Val Ser Asp Pro Ala Gly Ser Asp Ala Leu           
        665                 670                 675                       

aac gtt ttg aaa tat ttc gac tac aac gtt ttc cat gtt cca acc tgt       2477
Asn Val Leu Lys Tyr Phe Asp Tyr Asn Val Phe His Val Pro Thr Cys           
    680                 685                 690                           

aaa ttg gtc aca aaa tca tac ggt aga tta cta tat aac gat ttc aga       2525
Lys Leu Val Thr Lys Ser Tyr Gly Arg Leu Leu Tyr Asn Asp Phe Arg           
695                 700                 705                 710           

gcc aat cct caa ttg ttc cca gaa gtt gac gcc gaa tta gct act cgc       2573
Ala Asn Pro Gln Leu Phe Pro Glu Val Asp Ala Glu Leu Ala Thr Arg           
                715                 720                 725               

gat tat gac gaa tct tta acc gat aag aac att gaa aaa act ttt gtt       2621
Asp Tyr Asp Glu Ser Leu Thr Asp Lys Asn Ile Glu Lys Thr Phe Val           
            730                 735                 740                   

aat gtt gct aag cca ttc cac aaa gag aga gtt gcc caa tct ttg att       2669
Asn Val Ala Lys Pro Phe His Lys Glu Arg Val Ala Gln Ser Leu Ile           
        745                 750                 755                       

gtt cca aca aac aca ggt aac atg tac acc gca tct gtt tat gcc gcc       2717
Val Pro Thr Asn Thr Gly Asn Met Tyr Thr Ala Ser Val Tyr Ala Ala           
    760                 765                 770                           

ttt gca tct cta tta aac tat gtt gga tct gac gac tta caa ggc aag       2765
Phe Ala Ser Leu Leu Asn Tyr Val Gly Ser Asp Asp Leu Gln Gly Lys           
775                 780                 785                 790           

cgt gtt ggt tta ttt tct tac ggt tcc ggt tta gct gca tct cta tat       2813
Arg Val Gly Leu Phe Ser Tyr Gly Ser Gly Leu Ala Ala Ser Leu Tyr           
                795                 800                 805               

tct tgc aaa att gtt ggt gac gtc caa cat att atc aag gaa tta gat       2861
Ser Cys Lys Ile Val Gly Asp Val Gln His Ile Ile Lys Glu Leu Asp           
            810                 815                 820                   

att act aac aaa tta gcc aag aga atc acc gaa act cca aag gat tac       2909
Ile Thr Asn Lys Leu Ala Lys Arg Ile Thr Glu Thr Pro Lys Asp Tyr           
        825                 830                 835                       

gaa gct gcc atc gaa ttg aga gaa aat gcc cat ttg aag aag aac ttc       2957
Glu Ala Ala Ile Glu Leu Arg Glu Asn Ala His Leu Lys Lys Asn Phe           
    840                 845                 850                           

aaa cct caa ggt tcc att gag cat ttg caa agt ggt gtt tac tac ttg       3005
Lys Pro Gln Gly Ser Ile Glu His Leu Gln Ser Gly Val Tyr Tyr Leu           
855                 860                 865                 870           

acc aac atc gat gac aaa ttt aga aga tct tac gat gtt aaa aaa taa       3053
Thr Asn Ile Asp Asp Lys Phe Arg Arg Ser Tyr Asp Val Lys Lys               
                875                 880                 885               

ggaggattac act atg gtt tta acc aat aaa aca gtc att tct gga tcg        3102
               Met Val Leu Thr Asn Lys Thr Val Ile Ser Gly Ser            
                               890                 895                    

aaa gtc aaa agt tta tca tct gcg caa tcg agc tca tca gga cct tca       3150
Lys Val Lys Ser Leu Ser Ser Ala Gln Ser Ser Ser Ser Gly Pro Ser           
        900                 905                 910                       

tca tct agt gag gaa gat gat tcc cgc gat att gaa agc ttg gat aag       3198
Ser Ser Ser Glu Glu Asp Asp Ser Arg Asp Ile Glu Ser Leu Asp Lys           
    915                 920                 925                           

aaa ata cgt cct tta gaa gaa tta gaa gca tta tta agt agt gga aat       3246
Lys Ile Arg Pro Leu Glu Glu Leu Glu Ala Leu Leu Ser Ser Gly Asn           
930                 935                 940                 945           

aca aaa caa ttg aag aac aaa gag gtc gct gcc ttg gtt att cac ggt       3294
Thr Lys Gln Leu Lys Asn Lys Glu Val Ala Ala Leu Val Ile His Gly           
                950                 955                 960               

aag tta cct ttg tac gct ttg gag aaa aaa tta ggt gat act acg aga       3342
Lys Leu Pro Leu Tyr Ala Leu Glu Lys Lys Leu Gly Asp Thr Thr Arg           
            965                 970                 975                   

gcg gtt gcg gta cgt agg aag gct ctt tca att ttg gca gaa gct cct       3390
Ala Val Ala Val Arg Arg Lys Ala Leu Ser Ile Leu Ala Glu Ala Pro           
        980                 985                 990                       

gta tta gca tct gat cgt tta  cca tat aaa aat tat  gac tac gac cgc     3438
Val Leu Ala Ser Asp Arg Leu  Pro Tyr Lys Asn Tyr  Asp Tyr Asp Arg         
    995                 1000                 1005                         

gta  ttt ggc gct tgt tgt  gaa aat gtt ata ggt  tac atg cct ttg        3483
Val  Phe Gly Ala Cys Cys  Glu Asn Val Ile Gly  Tyr Met Pro Leu            
1010                 1015                 1020                            

ccc  gtt ggt gtt ata ggc  ccc ttg gtt atc gat  ggt aca tct tat        3528
Pro  Val Gly Val Ile Gly  Pro Leu Val Ile Asp  Gly Thr Ser Tyr            
1025                 1030                 1035                            

cat  ata cca atg gca act  aca gag ggt tgt ttg  gta gct tct gcc        3573
His  Ile Pro Met Ala Thr  Thr Glu Gly Cys Leu  Val Ala Ser Ala            
1040                 1045                 1050                            

atg  cgt ggc tgt aag gca  atc aat gct ggc ggt  ggt gca aca act        3618
Met  Arg Gly Cys Lys Ala  Ile Asn Ala Gly Gly  Gly Ala Thr Thr            
1055                 1060                 1065                            

gtt  tta act aag gat ggt  atg aca aga ggc cca  gta gtc cgt ttc        3663
Val  Leu Thr Lys Asp Gly  Met Thr Arg Gly Pro  Val Val Arg Phe            
1070                 1075                 1080                            

cca  act ttg aaa aga tct  ggt gcc tgt aag ata  tgg tta gac tca        3708
Pro  Thr Leu Lys Arg Ser  Gly Ala Cys Lys Ile  Trp Leu Asp Ser            
1085                 1090                 1095                            

gaa  gag gga caa aac gca  att aaa aaa gct ttt  aac tct aca tca        3753
Glu  Glu Gly Gln Asn Ala  Ile Lys Lys Ala Phe  Asn Ser Thr Ser            
1100                 1105                 1110                            

aga  ttt gca cgt ctg caa  cat att caa act tgt  cta gca gga gat        3798
Arg  Phe Ala Arg Leu Gln  His Ile Gln Thr Cys  Leu Ala Gly Asp            
1115                 1120                 1125                            

tta  ctc ttc atg aga ttt  aga aca act act ggt  gac gca atg ggt        3843
Leu  Leu Phe Met Arg Phe  Arg Thr Thr Thr Gly  Asp Ala Met Gly            
1130                 1135                 1140                            

atg  aat atg att tct aaa  ggt gtc gaa tac tca  tta aag caa atg        3888
Met  Asn Met Ile Ser Lys  Gly Val Glu Tyr Ser  Leu Lys Gln Met            
1145                 1150                 1155                            

gta  gaa gag tat ggc tgg  gaa gat atg gag gtt  gtc tcc gtt tct        3933
Val  Glu Glu Tyr Gly Trp  Glu Asp Met Glu Val  Val Ser Val Ser            
1160                 1165                 1170                            

ggt  aac tac tgt acc gac  aaa aaa cca gct gcc  atc aac tgg atc        3978
Gly  Asn Tyr Cys Thr Asp  Lys Lys Pro Ala Ala  Ile Asn Trp Ile            
1175                 1180                 1185                            

gaa  ggt cgt ggt aag agt  gtc gtc gca gaa gct  act att cct ggt        4023
Glu  Gly Arg Gly Lys Ser  Val Val Ala Glu Ala  Thr Ile Pro Gly            
1190                 1195                 1200                            

gat  gtt gtc aga aaa gtg  tta aaa agt gat gtt  tcc gca ttg gtt        4068
Asp  Val Val Arg Lys Val  Leu Lys Ser Asp Val  Ser Ala Leu Val            
1205                 1210                 1215                            

gag  ttg aac att gct aag  aat ttg gtt gga tct  gca atg gct ggg        4113
Glu  Leu Asn Ile Ala Lys  Asn Leu Val Gly Ser  Ala Met Ala Gly            
1220                 1225                 1230                            

tct  gtt ggt gga ttt aac  gca cat gca gct aat  tta gtg aca gct        4158
Ser  Val Gly Gly Phe Asn  Ala His Ala Ala Asn  Leu Val Thr Ala            
1235                 1240                 1245                            

gtt  ttc ttg gca tta gga  caa gat cct gca caa  aat gtt gaa agt        4203
Val  Phe Leu Ala Leu Gly  Gln Asp Pro Ala Gln  Asn Val Glu Ser            
1250                 1255                 1260                            

tcc  aac tgt ata aca ttg  atg aaa gaa gtg gac  ggt gat ttg aga        4248
Ser  Asn Cys Ile Thr Leu  Met Lys Glu Val Asp  Gly Asp Leu Arg            
1265                 1270                 1275                            

att  tcc gta tcc atg cca  tcc atc gaa gta ggt  acc atc ggt ggt        4293
Ile  Ser Val Ser Met Pro  Ser Ile Glu Val Gly  Thr Ile Gly Gly            
1280                 1285                 1290                            

ggt  act gtt cta gaa cca  caa ggt gcc atg ttg  gac tta tta ggt        4338
Gly  Thr Val Leu Glu Pro  Gln Gly Ala Met Leu  Asp Leu Leu Gly            
1295                 1300                 1305                            

gta  aga ggc ccg cat gct  acc gct cct ggt acc  aac gca cgt caa        4383
Val  Arg Gly Pro His Ala  Thr Ala Pro Gly Thr  Asn Ala Arg Gln            
1310                 1315                 1320                            

tta  gca aga ata gtt gcc  tgt gcc gtc ttg gca  ggt gaa tta tcc        4428
Leu  Ala Arg Ile Val Ala  Cys Ala Val Leu Ala  Gly Glu Leu Ser            
1325                 1330                 1335                            

tta  tgt gct gcc cta gca  gcc ggc cat ttg gtt  caa agt cat atg        4473
Leu  Cys Ala Ala Leu Ala  Ala Gly His Leu Val  Gln Ser His Met            
1340                 1345                 1350                            

acc  cac aac agg aaa cct  gct gaa cca aca aaa  cct aac aat ttg        4518
Thr  His Asn Arg Lys Pro  Ala Glu Pro Thr Lys  Pro Asn Asn Leu            
1355                 1360                 1365                            

gac  gcc act gat ata aat  cgt ttg aaa gat ggg  tcc gtc acc tgc        4563
Asp  Ala Thr Asp Ile Asn  Arg Leu Lys Asp Gly  Ser Val Thr Cys            
1370                 1375                 1380                            

att  aaa tcc taa gtcgacctgc aggcatgcaa gcttggctgt tttggcggat          4615
Ile  Lys Ser                                                              
1385                                                                      

gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac     4675

agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag     4735

tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc     4795

aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt     4855

ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg     4915

aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt     4975

aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt     5035

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca     5095

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt     5155

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga     5215

tgctgaagat cagttgggtg cagcaaacta ttaactggcg aactacttac tctagcttcc     5275

cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg     5335

gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc     5395

ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg     5455

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca     5515

ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta     5575

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc     5635

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac     5695

gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag     5755

tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc     5815

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg     5875

actcttgttc caaacttgaa caacactcaa ccctatctcg ggctattctt ttgatttata     5935

agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa     5995

cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt     6055

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc     6115

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct     6175

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa     6235

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag     6295

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc     6355

tgctaatcct gttaccagtg gggcatttga gaagcacacg gtcacactgc ttccggtagt     6415

caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc     6475

gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt     6535

caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc     6595

cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat     6655

cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat     6715

aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat     6775

caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc     6835

ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta     6895

gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct     6955

catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca     7015

ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg     7075

gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa     7135

cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat     7195

gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct     7255

tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat     7315

ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc     7375

agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg     7435

tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt     7495

gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt     7555

tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg     7615

agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg     7675

caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc     7735

ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta     7795

cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg     7855

gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga     7915

cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     7975

ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg     8035

ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc     8095

caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa     8155

ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg     8215

taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga     8275

caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc     8335

agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta     8395

cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttgctca     8455

tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag     8515

caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctgct     8575

catgtttgac agcttatc                                                   8593


<210>  109
<211>  394
<212>  PRT
<213>  synthetic

<400>  109

Met Lys Asn Cys Val Ile Val Ser Ala Val Arg Thr Ala Ile Gly Ser 
1               5                   10                  15      


Phe Asn Gly Ser Leu Ala Ser Thr Ser Ala Ile Asp Leu Gly Ala Thr 
            20                  25                  30          


Val Ile Lys Ala Ala Ile Glu Arg Ala Lys Ile Asp Ser Gln His Val 
        35                  40                  45              


Asp Glu Val Ile Met Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Leu Leu Lys Ser Gly Leu Ala Glu Thr Val Cys 
65                  70                  75                  80  


Gly Phe Thr Val Asn Lys Val Cys Gly Ser Gly Leu Lys Ser Val Ala 
                85                  90                  95      


Leu Ala Ala Gln Ala Ile Gln Ala Gly Gln Ala Gln Ser Ile Val Ala 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Leu Ala Pro Tyr Leu Leu Asp Ala Lys 
        115                 120                 125             


Ala Arg Ser Gly Tyr Arg Leu Gly Asp Gly Gln Val Tyr Asp Val Ile 
    130                 135                 140                 


Leu Arg Asp Gly Leu Met Cys Ala Thr His Gly Tyr His Met Gly Ile 
145                 150                 155                 160 


Thr Ala Glu Asn Val Ala Lys Glu Tyr Gly Ile Thr Arg Glu Met Gln 
                165                 170                 175     


Asp Glu Leu Ala Leu His Ser Gln Arg Lys Ala Ala Ala Ala Ile Glu 
            180                 185                 190         


Ser Gly Ala Phe Thr Ala Glu Ile Val Pro Val Asn Val Val Thr Arg 
        195                 200                 205             


Lys Lys Thr Phe Val Phe Ser Gln Asp Glu Phe Pro Lys Ala Asn Ser 
    210                 215                 220                 


Thr Ala Glu Ala Leu Gly Ala Leu Arg Pro Ala Phe Asp Lys Ala Gly 
225                 230                 235                 240 


Thr Val Thr Ala Gly Asn Ala Ser Gly Ile Asn Asp Gly Ala Ala Ala 
                245                 250                 255     


Leu Val Ile Met Glu Glu Ser Ala Ala Leu Ala Ala Gly Leu Thr Pro 
            260                 265                 270         


Leu Ala Arg Ile Lys Ser Tyr Ala Ser Gly Gly Val Pro Pro Ala Leu 
        275                 280                 285             


Met Gly Met Gly Pro Val Pro Ala Thr Gln Lys Ala Leu Gln Leu Ala 
    290                 295                 300                 


Gly Leu Gln Leu Ala Asp Ile Asp Leu Ile Glu Ala Asn Glu Ala Phe 
305                 310                 315                 320 


Ala Ala Gln Phe Leu Ala Val Gly Lys Asn Leu Gly Phe Asp Ser Glu 
                325                 330                 335     


Lys Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly 
            340                 345                 350         


Ala Ser Gly Ala Arg Ile Leu Val Thr Leu Leu His Ala Met Gln Ala 
        355                 360                 365             


Arg Asp Lys Thr Leu Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Gln 
    370                 375                 380                 


Gly Ile Ala Met Val Ile Glu Arg Leu Asn 
385                 390                 


<210>  110
<211>  491
<212>  PRT
<213>  synthetic

<400>  110

Met Lys Leu Ser Thr Lys Leu Cys Trp Cys Gly Ile Lys Gly Arg Leu 
1               5                   10                  15      


Arg Pro Gln Lys Gln Gln Gln Leu His Asn Thr Asn Leu Gln Met Thr 
            20                  25                  30          


Glu Leu Lys Lys Gln Lys Thr Ala Glu Gln Lys Thr Arg Pro Gln Asn 
        35                  40                  45              


Val Gly Ile Lys Gly Ile Gln Ile Tyr Ile Pro Thr Gln Cys Val Asn 
    50                  55                  60                  


Gln Ser Glu Leu Glu Lys Phe Asp Gly Val Ser Gln Gly Lys Tyr Thr 
65                  70                  75                  80  


Ile Gly Leu Gly Gln Thr Asn Met Ser Phe Val Asn Asp Arg Glu Asp 
                85                  90                  95      


Ile Tyr Ser Met Ser Leu Thr Val Leu Ser Lys Leu Ile Lys Ser Tyr 
            100                 105                 110         


Asn Ile Asp Thr Asn Lys Ile Gly Arg Leu Glu Val Gly Thr Glu Thr 
        115                 120                 125             


Leu Ile Asp Lys Ser Lys Ser Val Lys Ser Val Leu Met Gln Leu Phe 
    130                 135                 140                 


Gly Glu Asn Thr Asp Val Glu Gly Ile Asp Thr Leu Asn Ala Cys Tyr 
145                 150                 155                 160 


Gly Gly Thr Asn Ala Leu Phe Asn Ser Leu Asn Trp Ile Glu Ser Asn 
                165                 170                 175     


Ala Trp Asp Gly Arg Asp Ala Ile Val Val Cys Gly Asp Ile Ala Ile 
            180                 185                 190         


Tyr Asp Lys Gly Ala Ala Arg Pro Thr Gly Gly Ala Gly Thr Val Ala 
        195                 200                 205             


Met Trp Ile Gly Pro Asp Ala Pro Ile Val Phe Asp Ser Val Arg Ala 
    210                 215                 220                 


Ser Tyr Met Glu His Ala Tyr Asp Phe Tyr Lys Pro Asp Phe Thr Ser 
225                 230                 235                 240 


Glu Tyr Pro Tyr Val Asp Gly His Phe Ser Leu Thr Cys Tyr Val Lys 
                245                 250                 255     


Ala Leu Asp Gln Val Tyr Lys Ser Tyr Ser Lys Lys Ala Ile Ser Lys 
            260                 265                 270         


Gly Leu Val Ser Asp Pro Ala Gly Ser Asp Ala Leu Asn Val Leu Lys 
        275                 280                 285             


Tyr Phe Asp Tyr Asn Val Phe His Val Pro Thr Cys Lys Leu Val Thr 
    290                 295                 300                 


Lys Ser Tyr Gly Arg Leu Leu Tyr Asn Asp Phe Arg Ala Asn Pro Gln 
305                 310                 315                 320 


Leu Phe Pro Glu Val Asp Ala Glu Leu Ala Thr Arg Asp Tyr Asp Glu 
                325                 330                 335     


Ser Leu Thr Asp Lys Asn Ile Glu Lys Thr Phe Val Asn Val Ala Lys 
            340                 345                 350         


Pro Phe His Lys Glu Arg Val Ala Gln Ser Leu Ile Val Pro Thr Asn 
        355                 360                 365             


Thr Gly Asn Met Tyr Thr Ala Ser Val Tyr Ala Ala Phe Ala Ser Leu 
    370                 375                 380                 


Leu Asn Tyr Val Gly Ser Asp Asp Leu Gln Gly Lys Arg Val Gly Leu 
385                 390                 395                 400 


Phe Ser Tyr Gly Ser Gly Leu Ala Ala Ser Leu Tyr Ser Cys Lys Ile 
                405                 410                 415     


Val Gly Asp Val Gln His Ile Ile Lys Glu Leu Asp Ile Thr Asn Lys 
            420                 425                 430         


Leu Ala Lys Arg Ile Thr Glu Thr Pro Lys Asp Tyr Glu Ala Ala Ile 
        435                 440                 445             


Glu Leu Arg Glu Asn Ala His Leu Lys Lys Asn Phe Lys Pro Gln Gly 
    450                 455                 460                 


Ser Ile Glu His Leu Gln Ser Gly Val Tyr Tyr Leu Thr Asn Ile Asp 
465                 470                 475                 480 


Asp Lys Phe Arg Arg Ser Tyr Asp Val Lys Lys 
                485                 490     


<210>  111
<211>  502
<212>  PRT
<213>  synthetic

<400>  111

Met Val Leu Thr Asn Lys Thr Val Ile Ser Gly Ser Lys Val Lys Ser 
1               5                   10                  15      


Leu Ser Ser Ala Gln Ser Ser Ser Ser Gly Pro Ser Ser Ser Ser Glu 
            20                  25                  30          


Glu Asp Asp Ser Arg Asp Ile Glu Ser Leu Asp Lys Lys Ile Arg Pro 
        35                  40                  45              


Leu Glu Glu Leu Glu Ala Leu Leu Ser Ser Gly Asn Thr Lys Gln Leu 
    50                  55                  60                  


Lys Asn Lys Glu Val Ala Ala Leu Val Ile His Gly Lys Leu Pro Leu 
65                  70                  75                  80  


Tyr Ala Leu Glu Lys Lys Leu Gly Asp Thr Thr Arg Ala Val Ala Val 
                85                  90                  95      


Arg Arg Lys Ala Leu Ser Ile Leu Ala Glu Ala Pro Val Leu Ala Ser 
            100                 105                 110         


Asp Arg Leu Pro Tyr Lys Asn Tyr Asp Tyr Asp Arg Val Phe Gly Ala 
        115                 120                 125             


Cys Cys Glu Asn Val Ile Gly Tyr Met Pro Leu Pro Val Gly Val Ile 
    130                 135                 140                 


Gly Pro Leu Val Ile Asp Gly Thr Ser Tyr His Ile Pro Met Ala Thr 
145                 150                 155                 160 


Thr Glu Gly Cys Leu Val Ala Ser Ala Met Arg Gly Cys Lys Ala Ile 
                165                 170                 175     


Asn Ala Gly Gly Gly Ala Thr Thr Val Leu Thr Lys Asp Gly Met Thr 
            180                 185                 190         


Arg Gly Pro Val Val Arg Phe Pro Thr Leu Lys Arg Ser Gly Ala Cys 
        195                 200                 205             


Lys Ile Trp Leu Asp Ser Glu Glu Gly Gln Asn Ala Ile Lys Lys Ala 
    210                 215                 220                 


Phe Asn Ser Thr Ser Arg Phe Ala Arg Leu Gln His Ile Gln Thr Cys 
225                 230                 235                 240 


Leu Ala Gly Asp Leu Leu Phe Met Arg Phe Arg Thr Thr Thr Gly Asp 
                245                 250                 255     


Ala Met Gly Met Asn Met Ile Ser Lys Gly Val Glu Tyr Ser Leu Lys 
            260                 265                 270         


Gln Met Val Glu Glu Tyr Gly Trp Glu Asp Met Glu Val Val Ser Val 
        275                 280                 285             


Ser Gly Asn Tyr Cys Thr Asp Lys Lys Pro Ala Ala Ile Asn Trp Ile 
    290                 295                 300                 


Glu Gly Arg Gly Lys Ser Val Val Ala Glu Ala Thr Ile Pro Gly Asp 
305                 310                 315                 320 


Val Val Arg Lys Val Leu Lys Ser Asp Val Ser Ala Leu Val Glu Leu 
                325                 330                 335     


Asn Ile Ala Lys Asn Leu Val Gly Ser Ala Met Ala Gly Ser Val Gly 
            340                 345                 350         


Gly Phe Asn Ala His Ala Ala Asn Leu Val Thr Ala Val Phe Leu Ala 
        355                 360                 365             


Leu Gly Gln Asp Pro Ala Gln Asn Val Glu Ser Ser Asn Cys Ile Thr 
    370                 375                 380                 


Leu Met Lys Glu Val Asp Gly Asp Leu Arg Ile Ser Val Ser Met Pro 
385                 390                 395                 400 


Ser Ile Glu Val Gly Thr Ile Gly Gly Gly Thr Val Leu Glu Pro Gln 
                405                 410                 415     


Gly Ala Met Leu Asp Leu Leu Gly Val Arg Gly Pro His Ala Thr Ala 
            420                 425                 430         


Pro Gly Thr Asn Ala Arg Gln Leu Ala Arg Ile Val Ala Cys Ala Val 
        435                 440                 445             


Leu Ala Gly Glu Leu Ser Leu Cys Ala Ala Leu Ala Ala Gly His Leu 
    450                 455                 460                 


Val Gln Ser His Met Thr His Asn Arg Lys Pro Ala Glu Pro Thr Lys 
465                 470                 475                 480 


Pro Asn Asn Leu Asp Ala Thr Asp Ile Asn Arg Leu Lys Asp Gly Ser 
                485                 490                 495     


Val Thr Cys Ile Lys Ser 
            500         


<210>  112
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  yccV forward primer

<400>  112
attcatatgu ggttataaag acatcaacat gc                                     32


<210>  113
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  yccV reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  113
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt agtcacctcc cgggaaatct                                        90


<210>  114
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  ycjX forward primer

<400>  114
attcatatgu ttggctgata ctgtaattct tc                                     32


<210>  115
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  ycjX reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  115
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt tgctgtcctg tgctgctctg                                        90


<210>  116
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  mutM forward primer

<400>  116
attcatatgu ggcgaaaagt actaagtact ta                                     32


<210>  117
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  mutM reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  117
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt agcatctcca ggaatgaaca                                        90


<210>  118
<211>  19
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(19)
<223>  primer P492

<400>  118
tgcaactctt cttcaacgc                                                    19


<210>  119
<211>  19
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(19)
<223>  primer P493

<400>  119
aagaaccagg cgcttctaa                                                    19


<210>  120
<211>  19
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(19)
<223>  primer P525

<400>  120
attgatgacc tgatggcac                                                    19


<210>  121
<211>  18
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(18)
<223>  primer P526

<400>  121
gatccagact tcgaagcg                                                     18


<210>  122
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  ibpA forward primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  122
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt aatcaatagc tcctgaaatc                                        90


<210>  123
<211>  31
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(31)
<223>  ibpA reverse primer

<400>  123
attcatatgt ttggcctgat gagttatagc g                                      31


<210>  124
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  grpE forward primer

<400>  124
attcatatgu tctccgcgag cgtgccagtt tt                                     32


<210>  125
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  grpE reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  125
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt gaatttctcc gcgttttttt                                        90


<210>  126
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  ldhA forward primer

<400>  126
attcatatgu gggaacccac agcccgagcg tc                                     32


<210>  127
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  ldhA reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  127
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt aagactttct ccagtgatgt                                        90


<210>  128
<211>  122
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(122)
<223>  ybbN forward primer

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  n is a, c, g, t or u

<400>  128
attcatatgu cccattcgta ctcgcttcac cgatccccat tacgtgagga tggctaaggt       60

ccagtgaagt accctgggca aagagtttca taccttncaa gtggagtcgc tctctgttgt      120

cg                                                                     122


<210>  129
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  ybbN reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  129
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt ggagtcgctc tctgttgtcg                                        90


<210>  130
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  fxsA forward primer

<400>  130
attcatatgu taaacacaca gaatatatgt gg                                     32


<210>  131
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  fxsA reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  131
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt aggtttctcc tgtaatagca                                        90


<210>  132
<211>  31
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(31)
<223>  rrnB forward primer

<400>  132
attcatatgu gcaaacacgc cgccgggtca g                                      31


<210>  133
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  rrnB reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  133
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt aaaagtttga cgctcaaaga                                        90


<210>  134
<211>  35
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(35)
<223>  rrnE forward primer

<400>  134
attcatatgu aatgcgcctc cgttgagacg acaac                                  35


<210>  135
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  rrnE reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  135
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt aaaagtttga tgctcaaaga                                        90


<210>  136
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  poxB forwrad primer

<400>  136
attcatatgu taggttgtcg ctgcctgccg tg                                     32


<210>  137
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  poxB reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  137
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt ggttctccat ctcctgaatg                                        90


<210>  138
<211>  32
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(32)
<223>  cspD forward primer

<400>  138
attcatatgu cggttatcgg cagaacgccc tg                                     32


<210>  139
<211>  90
<212>  DNA
<213>  synthetic


<220>
<221>  primer
<222>  (1)..(90)
<223>  cspD reverse primer

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, c, g, or t

<400>  139
atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcata       60

ccttncaagt gcttcgacat ccttcgcaaa                                        90


<210>  140
<211>  1902
<212>  DNA
<213>  synthetic


<220>
<221>  integration sequence s9_pmutM_folP
<222>  (1)..(1902)

<220>
<221>  CDS
<222>  (299)..(1093)
<223>  kanR

<220>
<221>  promoter
<222>  (1533)..(1832)
<223>  burden sensing promoter p(mutM)

<220>
<221>  RBS
<222>  (1833)..(1838)
<223>  rbs catalog

<220>
<221>  misc_feature
<222>  (1838)..(1838)
<223>  n is a, c, g, or t

<220>
<221>  gene
<222>  (1844)..(1902)
<223>  beginning of folP gene

<400>  140
aagaaccagg cgcttctaac aattctggcg acaatggtag tccaaaggct cctcgtccgg       60

ttgatgaacc gcgtacgccg aacccgggta acaccatgtc agagcagtta ggcgacaagt      120

aagttcccgc atcagatgac tgtatttgta ccgaaaaccc cggggcgtgc tccggggttt      180

tttcttatca attcatacca gggataacat cagcgattgt gtaggctgga gctgcttcga      240

agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tcccctta        298

tta gaa gaa ctc gtc aag aag gcg ata gaa ggc gat gcg ctg cga atc        346
Leu Glu Glu Leu Val Lys Lys Ala Ile Glu Gly Asp Ala Leu Arg Ile           
1               5                   10                  15                

ggg agc ggc gat acc gta aag cac gag gaa gcg gtc agc cca ttc gcc        394
Gly Ser Gly Asp Thr Val Lys His Glu Glu Ala Val Ser Pro Phe Ala           
            20                  25                  30                    

gcc aag ctc ttc agc aat atc acg ggt agc caa cgc tat gtc ctg ata        442
Ala Lys Leu Phe Ser Asn Ile Thr Gly Ser Gln Arg Tyr Val Leu Ile           
        35                  40                  45                        

gcg gtc cgc cac acc cag ccg gcc aca gtc gat gaa tcc aga aaa gcg        490
Ala Val Arg His Thr Gln Pro Ala Thr Val Asp Glu Ser Arg Lys Ala           
    50                  55                  60                            

gcc att ttc cac cat gat att cgg caa gca ggc atc gcc atg ggt cac        538
Ala Ile Phe His His Asp Ile Arg Gln Ala Gly Ile Ala Met Gly His           
65                  70                  75                  80            

gac gag atc ctc gcc gtc ggg cat gcg cgc ctt gag cct ggc gaa cag        586
Asp Glu Ile Leu Ala Val Gly His Ala Arg Leu Glu Pro Gly Glu Gln           
                85                  90                  95                

ttc ggc tgg cgc gag ccc ctg atg ctc ttc gtc cag atc atc ctg atc        634
Phe Gly Trp Arg Glu Pro Leu Met Leu Phe Val Gln Ile Ile Leu Ile           
            100                 105                 110                   

gac aag acc ggc ttc cat ccg agt acg tgc tcg ctc gat gcg atg ttt        682
Asp Lys Thr Gly Phe His Pro Ser Thr Cys Ser Leu Asp Ala Met Phe           
        115                 120                 125                       

cgc ttg gtg gtc gaa tgg gca ggt agc cgg atc aag cgt atg cag ccg        730
Arg Leu Val Val Glu Trp Ala Gly Ser Arg Ile Lys Arg Met Gln Pro           
    130                 135                 140                           

ccg cat tgc atc agc cat gat gga tac ttt ctc ggc agg agc aag gtg        778
Pro His Cys Ile Ser His Asp Gly Tyr Phe Leu Gly Arg Ser Lys Val           
145                 150                 155                 160           

aga tga cag gag atc ctg ccc cgg cac ttc gcc caa tag cag cca gtc        826
Arg     Gln Glu Ile Leu Pro Arg His Phe Ala Gln     Gln Pro Val           
                    165                 170                               

cct tcc cgc ttc agt gac aac gtc gag cac agc tgc gca agg aac gcc        874
Pro Ser Arg Phe Ser Asp Asn Val Glu His Ser Cys Ala Arg Asn Ala           
175                 180                 185                 190           

cgt cgt ggc cag cca cga tag ccg cgc tgc ctc gtc ctg cag ttc att        922
Arg Arg Gly Gln Pro Arg     Pro Arg Cys Leu Val Leu Gln Phe Ile           
                195                     200                 205           

cag ggc acc gga cag gtc ggt ctt gac aaa aag aac cgg gcg ccc ctg        970
Gln Gly Thr Gly Gln Val Gly Leu Asp Lys Lys Asn Arg Ala Pro Leu           
                210                 215                 220               

cgc tga cag ccg gaa cac ggc ggc atc aga gca gcc gat tgt ctg ttg       1018
Arg     Gln Pro Glu His Gly Gly Ile Arg Ala Ala Asp Cys Leu Leu           
                225                 230                 235               

tgc cca gtc ata gcc gaa tag cct ctc cac cca agc ggc cgg aga acc       1066
Cys Pro Val Ile Ala Glu     Pro Leu His Pro Ser Gly Arg Arg Thr           
            240                     245                 250               

tgc gtg caa tcc atc ttg ttc aat cat gcgaaacgat cctcatcctg             1113
Cys Val Gln Ser Ile Leu Phe Asn His                                       
            255                 260                                       

tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc     1173

agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt     1233

cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta     1293

cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca     1353

tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc     1413

ccttgcgccc tgagtgcttg cggcagcgtg agcttcaaaa gcgctctgaa gttcctatac     1473

tttctagaga ataggaactt cgaactgcag gtcgacggat ccccggaata ttcatatgtg     1533

gcgaaaagta ctaagtactt agaggaaata aatcatggct aaaggtattc gtgagaaaat     1593

caagctggtt tcttctgctg gtactggtca cttctatacc actacgaaga acaaacgtac     1653

taagccggaa aaactggaac tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta     1713

caaagaagcg aaaatcaaat aattctcgct ttgatgtaac aaaaaacctc gctccggcgg     1773

ggttttttgt tatctgcttg cccccatatt gactgcatct gttcattcct ggagatgcta     1833

cttgnaaggt atgaaactct ttgcccaggg tacttcactg gaccttagcc atcctcacgt     1893

aatggggat                                                             1902


<210>  141
<211>  161
<212>  PRT
<213>  synthetic

<400>  141

Leu Glu Glu Leu Val Lys Lys Ala Ile Glu Gly Asp Ala Leu Arg Ile 
1               5                   10                  15      


Gly Ser Gly Asp Thr Val Lys His Glu Glu Ala Val Ser Pro Phe Ala 
            20                  25                  30          


Ala Lys Leu Phe Ser Asn Ile Thr Gly Ser Gln Arg Tyr Val Leu Ile 
        35                  40                  45              


Ala Val Arg His Thr Gln Pro Ala Thr Val Asp Glu Ser Arg Lys Ala 
    50                  55                  60                  


Ala Ile Phe His His Asp Ile Arg Gln Ala Gly Ile Ala Met Gly His 
65                  70                  75                  80  


Asp Glu Ile Leu Ala Val Gly His Ala Arg Leu Glu Pro Gly Glu Gln 
                85                  90                  95      


Phe Gly Trp Arg Glu Pro Leu Met Leu Phe Val Gln Ile Ile Leu Ile 
            100                 105                 110         


Asp Lys Thr Gly Phe His Pro Ser Thr Cys Ser Leu Asp Ala Met Phe 
        115                 120                 125             


Arg Leu Val Val Glu Trp Ala Gly Ser Arg Ile Lys Arg Met Gln Pro 
    130                 135                 140                 


Pro His Cys Ile Ser His Asp Gly Tyr Phe Leu Gly Arg Ser Lys Val 
145                 150                 155                 160 


Arg 
    


<210>  142
<211>  10
<212>  PRT
<213>  synthetic

<400>  142

Gln Glu Ile Leu Pro Arg His Phe Ala Gln 
1               5                   10  


<210>  143
<211>  25
<212>  PRT
<213>  synthetic

<400>  143

Gln Pro Val Pro Ser Arg Phe Ser Asp Asn Val Glu His Ser Cys Ala 
1               5                   10                  15      


Arg Asn Ala Arg Arg Gly Gln Pro Arg 
            20                  25  


<210>  144
<211>  26
<212>  PRT
<213>  synthetic

<400>  144

Pro Arg Cys Leu Val Leu Gln Phe Ile Gln Gly Thr Gly Gln Val Gly 
1               5                   10                  15      


Leu Asp Lys Lys Asn Arg Ala Pro Leu Arg 
            20                  25      


<210>  145
<211>  20
<212>  PRT
<213>  synthetic

<400>  145

Gln Pro Glu His Gly Gly Ile Arg Ala Ala Asp Cys Leu Leu Cys Pro 
1               5                   10                  15      


Val Ile Ala Glu 
            20  


<210>  146
<211>  18
<212>  PRT
<213>  synthetic

<400>  146

Pro Leu His Pro Ser Gly Arg Arg Thr Cys Val Gln Ser Ile Leu Phe 
1               5                   10                  15      


Asn His 
        


<210>  147
<211>  1347
<212>  DNA
<213>  Bacillus licheniformis


<220>
<221>  CDS
<222>  (1)..(1347)
<223>  Bacillus licheniformis essential gene glmM

<400>  147
atg ggc aag tat ttt ggt aca gac ggt gta aga ggc gtg gca aac agt         48
Met Gly Lys Tyr Phe Gly Thr Asp Gly Val Arg Gly Val Ala Asn Ser           
1               5                   10                  15                

gaa ctt aca cct gag ctg gcc ttt aaa gtc gga cgc ttt ggc gga tat         96
Glu Leu Thr Pro Glu Leu Ala Phe Lys Val Gly Arg Phe Gly Gly Tyr           
            20                  25                  30                    

gtc cta aca aaa gat aag gag cgt ccc aag gtt ctg atc ggc cgt gac        144
Val Leu Thr Lys Asp Lys Glu Arg Pro Lys Val Leu Ile Gly Arg Asp           
        35                  40                  45                        

acg cgc att tca ggg cat atg ctg gaa ggg gcc ctt gta gca ggc ctt        192
Thr Arg Ile Ser Gly His Met Leu Glu Gly Ala Leu Val Ala Gly Leu           
    50                  55                  60                            

ctt tcg ata gga gca gaa gtc atg cgt ctc ggc gtg att tcg acg ccc        240
Leu Ser Ile Gly Ala Glu Val Met Arg Leu Gly Val Ile Ser Thr Pro           
65                  70                  75                  80            

gga gtc gca tat ttg acg aag gct atg gac gcg gag gcg ggt gtg atg        288
Gly Val Ala Tyr Leu Thr Lys Ala Met Asp Ala Glu Ala Gly Val Met           
                85                  90                  95                

att tcc gct tcc cac aac cct gtc cag gac aac gga att aag ttt ttc        336
Ile Ser Ala Ser His Asn Pro Val Gln Asp Asn Gly Ile Lys Phe Phe           
            100                 105                 110                   

ggc ggc gac ggc ttt aag ctt tcc gat gaa cag gag ctt gaa atc gag        384
Gly Gly Asp Gly Phe Lys Leu Ser Asp Glu Gln Glu Leu Glu Ile Glu           
        115                 120                 125                       

cgt ctg atg gat cag ccg gaa gat cac ctg cca agg cct gta ggc gct        432
Arg Leu Met Asp Gln Pro Glu Asp His Leu Pro Arg Pro Val Gly Ala           
    130                 135                 140                           

gat ctg ggc atg gtg aac gac tac ttt gaa ggc gga cag aaa tat ttg        480
Asp Leu Gly Met Val Asn Asp Tyr Phe Glu Gly Gly Gln Lys Tyr Leu           
145                 150                 155                 160           

cag ttt ctt aag cag tct gct gat gaa gac ttc aca ggc att cat gtc        528
Gln Phe Leu Lys Gln Ser Ala Asp Glu Asp Phe Thr Gly Ile His Val           
                165                 170                 175               

gcg ctt gac tgt gcg cac ggg gcg aca tct tcg ctg gcg acc cac ctg        576
Ala Leu Asp Cys Ala His Gly Ala Thr Ser Ser Leu Ala Thr His Leu           
            180                 185                 190                   

ttt gcc gat ctt gac gca gat gta tcg acg atg ggg act tct ccg aac        624
Phe Ala Asp Leu Asp Ala Asp Val Ser Thr Met Gly Thr Ser Pro Asn           
        195                 200                 205                       

gga tta aac atc aat gac gga gtc gga tca acc cac cct gaa gcc ctg        672
Gly Leu Asn Ile Asn Asp Gly Val Gly Ser Thr His Pro Glu Ala Leu           
    210                 215                 220                           

gca gaa ttt gtg aag gaa aag gga gct gac gtc ggc atg gcg ttt gac        720
Ala Glu Phe Val Lys Glu Lys Gly Ala Asp Val Gly Met Ala Phe Asp           
225                 230                 235                 240           

ggg gat ggc gac cgc tta atc gct gtc gac gaa aaa ggg aat atc gta        768
Gly Asp Gly Asp Arg Leu Ile Ala Val Asp Glu Lys Gly Asn Ile Val           
                245                 250                 255               

gac ggc gat caa atc atg tac ata tgc gcg aag tac ttg aag agc gag        816
Asp Gly Asp Gln Ile Met Tyr Ile Cys Ala Lys Tyr Leu Lys Ser Glu           
            260                 265                 270                   

ggg cgc ctg acg gac aat acc gtt gtc tcc acg gtg atg agc aac ctc        864
Gly Arg Leu Thr Asp Asn Thr Val Val Ser Thr Val Met Ser Asn Leu           
        275                 280                 285                       

ggt ttc tac aaa gcg ctt gaa gcg gaa ggc att aaa agc gtt cag aca        912
Gly Phe Tyr Lys Ala Leu Glu Ala Glu Gly Ile Lys Ser Val Gln Thr           
    290                 295                 300                           

gcg gtc ggc gac cgc tat gta gtg gag gcg atg aaa aag ggc gga ttt        960
Ala Val Gly Asp Arg Tyr Val Val Glu Ala Met Lys Lys Gly Gly Phe           
305                 310                 315                 320           

acc ctc ggc gga gaa cag tcg gga cac ctg att ttc ctt gat tac aat       1008
Thr Leu Gly Gly Glu Gln Ser Gly His Leu Ile Phe Leu Asp Tyr Asn           
                325                 330                 335               

acg aca ggc gac gga ctg ctt tct gcg atc atg ctg atg aac acg att       1056
Thr Thr Gly Asp Gly Leu Leu Ser Ala Ile Met Leu Met Asn Thr Ile           
            340                 345                 350                   

aaa atg aca ggc aag ccg ctg tct gag ctt gcg gca gag atg cag aag       1104
Lys Met Thr Gly Lys Pro Leu Ser Glu Leu Ala Ala Glu Met Gln Lys           
        355                 360                 365                       

ttt ccg cag ctc ctc ttg aat gtg aaa gta acc gat aaa cat aaa gta       1152
Phe Pro Gln Leu Leu Leu Asn Val Lys Val Thr Asp Lys His Lys Val           
    370                 375                 380                           

acg gaa aac gaa aag gta aaa gcg gtc atc gaa gaa gtt gag aag gaa       1200
Thr Glu Asn Glu Lys Val Lys Ala Val Ile Glu Glu Val Glu Lys Glu           
385                 390                 395                 400           

atg aac ggt gac ggc cgc atc ctt gtg cgc cca tcc ggc acc gag ccg       1248
Met Asn Gly Asp Gly Arg Ile Leu Val Arg Pro Ser Gly Thr Glu Pro           
                405                 410                 415               

ctt gtc cgt gtc atg gct gaa gcg aaa acg aag gag ctt tgc gag aag       1296
Leu Val Arg Val Met Ala Glu Ala Lys Thr Lys Glu Leu Cys Glu Lys           
            420                 425                 430                   

tac gta ggc cgc att gca gat gtc gtg aaa gct gaa atg gga gca gag       1344
Tyr Val Gly Arg Ile Ala Asp Val Val Lys Ala Glu Met Gly Ala Glu           
        435                 440                 445                       

taa                                                                   1347


<210>  148
<211>  448
<212>  PRT
<213>  Bacillus licheniformis

<400>  148

Met Gly Lys Tyr Phe Gly Thr Asp Gly Val Arg Gly Val Ala Asn Ser 
1               5                   10                  15      


Glu Leu Thr Pro Glu Leu Ala Phe Lys Val Gly Arg Phe Gly Gly Tyr 
            20                  25                  30          


Val Leu Thr Lys Asp Lys Glu Arg Pro Lys Val Leu Ile Gly Arg Asp 
        35                  40                  45              


Thr Arg Ile Ser Gly His Met Leu Glu Gly Ala Leu Val Ala Gly Leu 
    50                  55                  60                  


Leu Ser Ile Gly Ala Glu Val Met Arg Leu Gly Val Ile Ser Thr Pro 
65                  70                  75                  80  


Gly Val Ala Tyr Leu Thr Lys Ala Met Asp Ala Glu Ala Gly Val Met 
                85                  90                  95      


Ile Ser Ala Ser His Asn Pro Val Gln Asp Asn Gly Ile Lys Phe Phe 
            100                 105                 110         


Gly Gly Asp Gly Phe Lys Leu Ser Asp Glu Gln Glu Leu Glu Ile Glu 
        115                 120                 125             


Arg Leu Met Asp Gln Pro Glu Asp His Leu Pro Arg Pro Val Gly Ala 
    130                 135                 140                 


Asp Leu Gly Met Val Asn Asp Tyr Phe Glu Gly Gly Gln Lys Tyr Leu 
145                 150                 155                 160 


Gln Phe Leu Lys Gln Ser Ala Asp Glu Asp Phe Thr Gly Ile His Val 
                165                 170                 175     


Ala Leu Asp Cys Ala His Gly Ala Thr Ser Ser Leu Ala Thr His Leu 
            180                 185                 190         


Phe Ala Asp Leu Asp Ala Asp Val Ser Thr Met Gly Thr Ser Pro Asn 
        195                 200                 205             


Gly Leu Asn Ile Asn Asp Gly Val Gly Ser Thr His Pro Glu Ala Leu 
    210                 215                 220                 


Ala Glu Phe Val Lys Glu Lys Gly Ala Asp Val Gly Met Ala Phe Asp 
225                 230                 235                 240 


Gly Asp Gly Asp Arg Leu Ile Ala Val Asp Glu Lys Gly Asn Ile Val 
                245                 250                 255     


Asp Gly Asp Gln Ile Met Tyr Ile Cys Ala Lys Tyr Leu Lys Ser Glu 
            260                 265                 270         


Gly Arg Leu Thr Asp Asn Thr Val Val Ser Thr Val Met Ser Asn Leu 
        275                 280                 285             


Gly Phe Tyr Lys Ala Leu Glu Ala Glu Gly Ile Lys Ser Val Gln Thr 
    290                 295                 300                 


Ala Val Gly Asp Arg Tyr Val Val Glu Ala Met Lys Lys Gly Gly Phe 
305                 310                 315                 320 


Thr Leu Gly Gly Glu Gln Ser Gly His Leu Ile Phe Leu Asp Tyr Asn 
                325                 330                 335     


Thr Thr Gly Asp Gly Leu Leu Ser Ala Ile Met Leu Met Asn Thr Ile 
            340                 345                 350         


Lys Met Thr Gly Lys Pro Leu Ser Glu Leu Ala Ala Glu Met Gln Lys 
        355                 360                 365             


Phe Pro Gln Leu Leu Leu Asn Val Lys Val Thr Asp Lys His Lys Val 
    370                 375                 380                 


Thr Glu Asn Glu Lys Val Lys Ala Val Ile Glu Glu Val Glu Lys Glu 
385                 390                 395                 400 


Met Asn Gly Asp Gly Arg Ile Leu Val Arg Pro Ser Gly Thr Glu Pro 
                405                 410                 415     


Leu Val Arg Val Met Ala Glu Ala Lys Thr Lys Glu Leu Cys Glu Lys 
            420                 425                 430         


Tyr Val Gly Arg Ile Ala Asp Val Val Lys Ala Glu Met Gly Ala Glu 
        435                 440                 445             


<210>  149
<211>  282
<212>  DNA
<213>  Bacillus licheniformis


<220>
<221>  CDS
<222>  (1)..(282)
<223>  Bacillus licheniformis essential gene ylaN

<400>  149
ttg gcg tct gag att gta gtc gac cat cgt gaa aaa gca ctt gct ctg         48
Leu Ala Ser Glu Ile Val Val Asp His Arg Glu Lys Ala Leu Ala Leu           
1               5                   10                  15                

tta aaa cgt gat gct gat aaa atc tta aag ctg att caa gtg caa atg         96
Leu Lys Arg Asp Ala Asp Lys Ile Leu Lys Leu Ile Gln Val Gln Met           
            20                  25                  30                    

gac aac tta acg atg cct caa tgt cct ctt tat gaa gag gtt tta gat        144
Asp Asn Leu Thr Met Pro Gln Cys Pro Leu Tyr Glu Glu Val Leu Asp           
        35                  40                  45                        

aca caa atg ttc gga ctc tct aga gaa att gat ttt gct gtc cgc ctt        192
Thr Gln Met Phe Gly Leu Ser Arg Glu Ile Asp Phe Ala Val Arg Leu           
    50                  55                  60                            

gga tta gtg gat gaa gaa gag ggt aaa gaa ctg ctt tac agg ctg gag        240
Gly Leu Val Asp Glu Glu Glu Gly Lys Glu Leu Leu Tyr Arg Leu Glu           
65                  70                  75                  80            

cgt gaa ttg tct gca tta cat gat gca ttt aca aaa aaa taa                282
Arg Glu Leu Ser Ala Leu His Asp Ala Phe Thr Lys Lys                       
                85                  90                                    


<210>  150
<211>  93
<212>  PRT
<213>  Bacillus licheniformis

<400>  150

Leu Ala Ser Glu Ile Val Val Asp His Arg Glu Lys Ala Leu Ala Leu 
1               5                   10                  15      


Leu Lys Arg Asp Ala Asp Lys Ile Leu Lys Leu Ile Gln Val Gln Met 
            20                  25                  30          


Asp Asn Leu Thr Met Pro Gln Cys Pro Leu Tyr Glu Glu Val Leu Asp 
        35                  40                  45              


Thr Gln Met Phe Gly Leu Ser Arg Glu Ile Asp Phe Ala Val Arg Leu 
    50                  55                  60                  


Gly Leu Val Asp Glu Glu Glu Gly Lys Glu Leu Leu Tyr Arg Leu Glu 
65                  70                  75                  80  


Arg Glu Leu Ser Ala Leu His Asp Ala Phe Thr Lys Lys 
                85                  90              


<210>  151
<211>  219
<212>  DNA
<213>  Bacillus licheniformis


<220>
<221>  CDS
<222>  (1)..(219)
<223>  Bacillus licheniformis essential gene infA

<400>  151
atg gcg aaa gac gat gta att gaa gtg gaa ggt act gta gtc gaa acg         48
Met Ala Lys Asp Asp Val Ile Glu Val Glu Gly Thr Val Val Glu Thr           
1               5                   10                  15                

ctg cca aac gca atg ttc aaa gtt gaa ctt gag aat ggc cac acg gtt         96
Leu Pro Asn Ala Met Phe Lys Val Glu Leu Glu Asn Gly His Thr Val           
            20                  25                  30                    

ttg gct cac gta tct ggt aaa atc cgc atg cac ttc att cgc att tta        144
Leu Ala His Val Ser Gly Lys Ile Arg Met His Phe Ile Arg Ile Leu           
        35                  40                  45                        

cct gga gac aaa gtt acg gta gaa tta tct cca tat gac tta act cgt        192
Pro Gly Asp Lys Val Thr Val Glu Leu Ser Pro Tyr Asp Leu Thr Arg           
    50                  55                  60                            

ggt aga att acg tac cgt tac aaa taa                                    219
Gly Arg Ile Thr Tyr Arg Tyr Lys                                           
65                  70                                                    


<210>  152
<211>  72
<212>  PRT
<213>  Bacillus licheniformis

<400>  152

Met Ala Lys Asp Asp Val Ile Glu Val Glu Gly Thr Val Val Glu Thr 
1               5                   10                  15      


Leu Pro Asn Ala Met Phe Lys Val Glu Leu Glu Asn Gly His Thr Val 
            20                  25                  30          


Leu Ala His Val Ser Gly Lys Ile Arg Met His Phe Ile Arg Ile Leu 
        35                  40                  45              


Pro Gly Asp Lys Val Thr Val Glu Leu Ser Pro Tyr Asp Leu Thr Arg 
    50                  55                  60                  


Gly Arg Ile Thr Tyr Arg Tyr Lys 
65                  70          


<210>  153
<211>  870
<212>  DNA
<213>  Bacillus licheniformis


<220>
<221>  CDS
<222>  (1)..(870)
<223>  Bacillus licheniformis essential gene dapA

<400>  153
atg aac ttc gga aat atc gca acc gcg atg gtt acg ccc ttt gac aaa         48
Met Asn Phe Gly Asn Ile Ala Thr Ala Met Val Thr Pro Phe Asp Lys           
1               5                   10                  15                

aat gaa aat atc gat ttt caa aaa ctg tca aag ctg atc gat tat tta         96
Asn Glu Asn Ile Asp Phe Gln Lys Leu Ser Lys Leu Ile Asp Tyr Leu           
            20                  25                  30                    

cta aac aat ggg acg gat tcc ctg gtc gtt gcc gga aca acc gga gaa        144
Leu Asn Asn Gly Thr Asp Ser Leu Val Val Ala Gly Thr Thr Gly Glu           
        35                  40                  45                        

tcg ccg acg ctt tcc gaa gaa gaa aag gta gcg ctc att caa tac tct        192
Ser Pro Thr Leu Ser Glu Glu Glu Lys Val Ala Leu Ile Gln Tyr Ser           
    50                  55                  60                            

gtc aag gaa gcg gca ggc cgt gcc cca atc atc gcc ggc acg gga agc        240
Val Lys Glu Ala Ala Gly Arg Ala Pro Ile Ile Ala Gly Thr Gly Ser           
65                  70                  75                  80            

aac aac acg aag gcg tcg atc aag ctg acg aaa aaa gct gaa gaa gcc        288
Asn Asn Thr Lys Ala Ser Ile Lys Leu Thr Lys Lys Ala Glu Glu Ala           
                85                  90                  95                

ggc gct gac gct gtc atg ctc gtc act ccg tac tac aac aag cct tct        336
Gly Ala Asp Ala Val Met Leu Val Thr Pro Tyr Tyr Asn Lys Pro Ser           
            100                 105                 110                   

cag gaa ggc atg tac cgc cat ttc aga gcg att gcg gag gaa aca tcg        384
Gln Glu Gly Met Tyr Arg His Phe Arg Ala Ile Ala Glu Glu Thr Ser           
        115                 120                 125                       

ctg cct gtc atg ctt tat aat gtg ccg gga aga acg gct gct tcg ctc        432
Leu Pro Val Met Leu Tyr Asn Val Pro Gly Arg Thr Ala Ala Ser Leu           
    130                 135                 140                           

gca cca gag acg aca atc cgc ctt gcg gag atc ccg aat att atc gcc        480
Ala Pro Glu Thr Thr Ile Arg Leu Ala Glu Ile Pro Asn Ile Ile Ala           
145                 150                 155                 160           

att aaa gaa gcg agc ggc gac cta gat gcg ata aca aaa atc gta gcc        528
Ile Lys Glu Ala Ser Gly Asp Leu Asp Ala Ile Thr Lys Ile Val Ala           
                165                 170                 175               

gaa acc cct gag gac ttt gca gtt tat tca gga gat gac agc ctg aca        576
Glu Thr Pro Glu Asp Phe Ala Val Tyr Ser Gly Asp Asp Ser Leu Thr           
            180                 185                 190                   

ctg ccg gcg ctg tcc gtg gga gcg aga gga atc gtt tcc gtt gca tca        624
Leu Pro Ala Leu Ser Val Gly Ala Arg Gly Ile Val Ser Val Ala Ser           
        195                 200                 205                       

cac atc atc gga ccg gaa atg cag gaa atg atc aaa cat tat acc gag        672
His Ile Ile Gly Pro Glu Met Gln Glu Met Ile Lys His Tyr Thr Glu           
    210                 215                 220                           

ggg aac acg gct cag gct gct ttg atc cat caa aag ctg ctt ccg ctg        720
Gly Asn Thr Ala Gln Ala Ala Leu Ile His Gln Lys Leu Leu Pro Leu           
225                 230                 235                 240           

atg aaa ggg ctt ttt gcc gca ccg aac ccg tct cca tta aag acg gct        768
Met Lys Gly Leu Phe Ala Ala Pro Asn Pro Ser Pro Leu Lys Thr Ala           
                245                 250                 255               

ctt cag ctg aag ggt ctt gac gtc ggg tcc gtc cgg ctc cct ttg att        816
Leu Gln Leu Lys Gly Leu Asp Val Gly Ser Val Arg Leu Pro Leu Ile           
            260                 265                 270                   

ccg cta aat gaa gat gaa cga ctc cgt tta agc agc ctg atg aac ggc        864
Pro Leu Asn Glu Asp Glu Arg Leu Arg Leu Ser Ser Leu Met Asn Gly           
        275                 280                 285                       

ctg taa                                                                870
Leu                                                                       
                                                                          


<210>  154
<211>  289
<212>  PRT
<213>  Bacillus licheniformis

<400>  154

Met Asn Phe Gly Asn Ile Ala Thr Ala Met Val Thr Pro Phe Asp Lys 
1               5                   10                  15      


Asn Glu Asn Ile Asp Phe Gln Lys Leu Ser Lys Leu Ile Asp Tyr Leu 
            20                  25                  30          


Leu Asn Asn Gly Thr Asp Ser Leu Val Val Ala Gly Thr Thr Gly Glu 
        35                  40                  45              


Ser Pro Thr Leu Ser Glu Glu Glu Lys Val Ala Leu Ile Gln Tyr Ser 
    50                  55                  60                  


Val Lys Glu Ala Ala Gly Arg Ala Pro Ile Ile Ala Gly Thr Gly Ser 
65                  70                  75                  80  


Asn Asn Thr Lys Ala Ser Ile Lys Leu Thr Lys Lys Ala Glu Glu Ala 
                85                  90                  95      


Gly Ala Asp Ala Val Met Leu Val Thr Pro Tyr Tyr Asn Lys Pro Ser 
            100                 105                 110         


Gln Glu Gly Met Tyr Arg His Phe Arg Ala Ile Ala Glu Glu Thr Ser 
        115                 120                 125             


Leu Pro Val Met Leu Tyr Asn Val Pro Gly Arg Thr Ala Ala Ser Leu 
    130                 135                 140                 


Ala Pro Glu Thr Thr Ile Arg Leu Ala Glu Ile Pro Asn Ile Ile Ala 
145                 150                 155                 160 


Ile Lys Glu Ala Ser Gly Asp Leu Asp Ala Ile Thr Lys Ile Val Ala 
                165                 170                 175     


Glu Thr Pro Glu Asp Phe Ala Val Tyr Ser Gly Asp Asp Ser Leu Thr 
            180                 185                 190         


Leu Pro Ala Leu Ser Val Gly Ala Arg Gly Ile Val Ser Val Ala Ser 
        195                 200                 205             


His Ile Ile Gly Pro Glu Met Gln Glu Met Ile Lys His Tyr Thr Glu 
    210                 215                 220                 


Gly Asn Thr Ala Gln Ala Ala Leu Ile His Gln Lys Leu Leu Pro Leu 
225                 230                 235                 240 


Met Lys Gly Leu Phe Ala Ala Pro Asn Pro Ser Pro Leu Lys Thr Ala 
                245                 250                 255     


Leu Gln Leu Lys Gly Leu Asp Val Gly Ser Val Arg Leu Pro Leu Ile 
            260                 265                 270         


Pro Leu Asn Glu Asp Glu Arg Leu Arg Leu Ser Ser Leu Met Asn Gly 
        275                 280                 285             


Leu 
    


<210>  155
<211>  2830
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(1000)
<223>  pAOX1 promoter

<220>
<221>  misc_feature
<222>  (1)..(2830)
<223>  pAOX1-ALB construct

<220>
<221>  CDS
<222>  (1001)..(2830)
<223>  cds encoding Human_serum_albumin (hAS) protein

<400>  155
atgttggtat tgtgaaatag acgcagatcg ggaacactga aaaataacag ttattattcg       60

agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag      120

gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt      180

tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc      240

agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta      300

acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta      360

tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg      420

agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct      480

gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg      540

ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt      600

cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct      660

ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct      720

ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact      780

gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat      840

atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt      900

actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga      960

caacttgaga agatcaaaaa acaactaatt attcgaaacg atg aag tgg gta acc       1015
                                            Met Lys Trp Val Thr           
                                            1               5             

ttt att tcc ctt ctt ttt ctc ttt agc tcg gct tat tcc agg ggt gtg       1063
Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala Tyr Ser Arg Gly Val           
                10                  15                  20                

ttt cgt cga gat gca cac aag agt gag gtt gct cat cgg ttt aaa gat       1111
Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp           
            25                  30                  35                    

ttg gga gaa gaa aat ttc aaa gcc ttg gtg ttg att gcc ttt gct cag       1159
Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln           
        40                  45                  50                        

tat ctt cag cag tgt cca ttt gaa gat cat gta aaa tta gtg aat gaa       1207
Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu           
    55                  60                  65                            

gta act gaa ttt gca aaa aca tgt gtt gct gat gag tca gct gaa aat       1255
Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn           
70                  75                  80                  85            

tgt gac aaa tca ctt cat acc ctt ttt gga gac aaa tta tgc aca gtt       1303
Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val           
                90                  95                  100               

gca act ctt cgt gaa acc tat ggt gaa atg gct gac tgc tgt gca aaa       1351
Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys           
            105                 110                 115                   

caa gaa cct gag aga aat gaa tgc ttc ttg caa cac aaa gat gac aac       1399
Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn           
        120                 125                 130                       

cca aac ctc ccc cga ttg gtg aga cca gag gtt gat gtg atg tgc act       1447
Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr           
    135                 140                 145                           

gct ttt cat gac aat gaa gag aca ttt ttg aaa aaa tac tta tat gaa       1495
Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu           
150                 155                 160                 165           

att gcc aga aga cat cct tac ttt tat gcc ccg gaa ctc ctt ttc ttt       1543
Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe           
                170                 175                 180               

gct aaa agg tat aaa gct gct ttt aca gaa tgt tgc caa gct gct gat       1591
Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp           
            185                 190                 195                   

aaa gct gcc tgc ctg ttg cca aag ctc gat gaa ctt cgg gat gaa ggg       1639
Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly           
        200                 205                 210                       

aag gct tcg tct gcc aaa cag aga ctc aag tgt gcc agt ctc caa aaa       1687
Lys Ala Ser Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys           
    215                 220                 225                           

ttt gga gaa aga gct ttc aaa gca tgg gca gta gct cgc ctg agc cag       1735
Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln           
230                 235                 240                 245           

aga ttt ccc aaa gct gag ttt gca gaa gtt tcc aag tta gtg aca gat       1783
Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp           
                250                 255                 260               

ctt acc aaa gtc cac acg gaa tgc tgc cat gga gat ctg ctt gaa tgt       1831
Leu Thr Lys Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys           
            265                 270                 275                   

gct gat gac agg gcg gac ctt gcc aag tat atc tgt gaa aat caa gat       1879
Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp           
        280                 285                 290                       

tcg atc tcc agt aaa ctg aag gaa tgc tgt gaa aaa cct ctg ttg gaa       1927
Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu           
    295                 300                 305                           

aaa tcc cac tgc att gcc gaa gtg gaa aat gat gag atg cct gct gac       1975
Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp           
310                 315                 320                 325           

ttg cct tca tta gct gct gat ttt gtt gaa agt aag gat gtt tgc aaa       2023
Leu Pro Ser Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys           
                330                 335                 340               

aac tat gct gag gca aag gat gtc ttc ctg ggc atg ttt ttg tat gaa       2071
Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu           
            345                 350                 355                   

tat gca aga agg cat cct gat tac tct gtc gtg ctg ctg ctg aga ctt       2119
Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu           
        360                 365                 370                       

gcc aag aca tat gaa acc act cta gag aag tgc tgt gcc gct gca gat       2167
Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp           
    375                 380                 385                           

cct cat gaa tgc tat gcc aaa gtg ttc gat gaa ttt aaa cct ctt gtg       2215
Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val           
390                 395                 400                 405           

gaa gag cct cag aat tta atc aaa caa aat tgt gag ctt ttt gag cag       2263
Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln           
                410                 415                 420               

ctt gga gag tac aaa ttc cag aat gcg cta tta gtt cgt tac acc aag       2311
Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys           
            425                 430                 435                   

aaa gta ccc caa gtg tca act cca act ctt gta gag gtc tca aga aac       2359
Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn           
        440                 445                 450                       

cta gga aaa gtg ggc agc aaa tgt tgt aaa cat cct gaa gca aaa aga       2407
Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg           
    455                 460                 465                           

atg ccc tgt gca gaa gac tat cta tcc gtg gtc ctg aac cag tta tgt       2455
Met Pro Cys Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys           
470                 475                 480                 485           

gtg ttg cat gag aaa acg cca gta agt gac aga gtc acc aaa tgc tgc       2503
Val Leu His Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys           
                490                 495                 500               

aca gaa tcc ttg gtg aac agg cga cca tgc ttt tca gct ctg gaa gtc       2551
Thr Glu Ser Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val           
            505                 510                 515                   

gat gaa aca tac gtt ccc aaa gag ttt aat gct gaa aca ttc acc ttc       2599
Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe           
        520                 525                 530                       

cat gca gat ata tgc aca ctt tct gag aag gag aga caa atc aag aaa       2647
His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys           
    535                 540                 545                           

caa act gca ctt gtt gag ctc gtg aaa cac aag ccc aag gca aca aaa       2695
Gln Thr Ala Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys           
550                 555                 560                 565           

gag caa ctg aaa gct gtt atg gat gat ttc gca gct ttt gta gag aag       2743
Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys           
                570                 575                 580               

tgc tgc aag gct gac gat aag gag acc tgc ttt gcc gag gag ggt aaa       2791
Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys           
            585                 590                 595                   

aaa ctt gtt gct gca agt caa gct gcc tta ggc tta taa                   2830
Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly Leu                           
        600                 605                                           


<210>  156
<211>  609
<212>  PRT
<213>  synthetic

<400>  156

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala 
            20                  25                  30          


His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu 
        35                  40                  45              


Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val 
    50                  55                  60                  


Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp 
65                  70                  75                  80  


Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp 
                85                  90                  95      


Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala 
            100                 105                 110         


Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln 
        115                 120                 125             


His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val 
    130                 135                 140                 


Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys 
145                 150                 155                 160 


Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro 
                165                 170                 175     


Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys 
            180                 185                 190         


Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu 
        195                 200                 205             


Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Arg Leu Lys Cys 
    210                 215                 220                 


Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val 
225                 230                 235                 240 


Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser 
                245                 250                 255     


Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu Cys Cys His Gly 
            260                 265                 270         


Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile 
        275                 280                 285             


Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu 
    290                 295                 300                 


Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp 
305                 310                 315                 320 


Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp Phe Val Glu Ser 
                325                 330                 335     


Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly 
            340                 345                 350         


Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val 
        355                 360                 365             


Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys 
    370                 375                 380                 


Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu 
385                 390                 395                 400 


Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys 
                405                 410                 415     


Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu 
            420                 425                 430         


Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val 
        435                 440                 445             


Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His 
    450                 455                 460                 


Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Tyr Leu Ser Val Val 
465                 470                 475                 480 


Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro Val Ser Asp Arg 
                485                 490                 495     


Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Arg Arg Pro Cys Phe 
            500                 505                 510         


Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala 
        515                 520                 525             


Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu 
    530                 535                 540                 


Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu Val Lys His Lys 
545                 550                 555                 560 


Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala 
                565                 570                 575     


Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe 
            580                 585                 590         


Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly 
        595                 600                 605             


Leu 
    


<210>  157
<211>  3505
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(3505)
<223>  construct KU70(up)-pPDI1-kanMX-terminator-KU70

<220>
<221>  misc_feature
<222>  (1)..(1002)
<223>  upstream region

<220>
<221>  promoter
<222>  (1002)..(1542)
<223>  promoter pPDI1

<220>
<221>  CDS
<222>  (1543)..(2352)
<223>  kanMX

<220>
<221>  terminator
<222>  (2353)..(2555)
<223>  terminator

<220>
<221>  misc_feature
<222>  (2556)..(3505)
<223>  downstream region

<400>  157
gctcttcaga agcctaactt taatggggta aaatatcagc agtaggctga atatagagca       60

tggcaacgtt tacgtcaaca gagtttaata gtgaaaacta cgataggttc aggcctgtat      120

acccagacga gttgtatcag caattagtgg attatcatgt tggtgctaaa ggattgtgtg      180

tcgatgttgg atgtggctct ggccaggcaa cgttcaccct gaaaaagtac tttgacaagg      240

tcataggatc tgatatctca gaaaatcagt tggcagtggc taggaaaaga cagcctgctg      300

gaattgaatt taggttagga acgggcgagg atttttcttg gctaacggag acgccagatg      360

taatcaccgc cgctgaatgc ctgcactggg tagacccaca gaaattcgtt gcaaatgtcg      420

ccaattctct aagggatcat ggcaccttga gttactggct ttatacagaa ccaatttttc      480

agaacgaaag ggccaatcag gtttacaaca aattcaccta cgggtctgac tacctgggcc      540

catattggga tcctggccgt acacatttca gaaatcattt aaaggagttg aatcacatct      600

tactggatag cgagcttttt gacgaagtga aaatttctaa ttttaaacaa gaggaagggg      660

tcaaaaacgg agatatctta tacttggaaa aagagatgac aatcagtgat ttcatcaatt      720

ttgtatctag ttggccttct gtgttttcgt ggaagcagca acgaggaaag gagggtatcc      780

tagatgattt ttacaacgaa ctgaacgact gctttgaggg gggtaacatg aaagtaatat      840

ggaactccgt cctagtattt gccaggagga agcaaagggt tgtataggct ttagtactta      900

tagaggaaac ggggttacgt gcaagcgcgc atgcctgagc tttgaggggg gggactttca      960

catctcttct tctcacactt agccctaaca cagagatcaa ctactcaagg cagcaccaaa     1020

agacgccaga aattatatgg tccaggaaaa aacaactctc tttactacaa aatttgaaca     1080

ggtgtcctgc ctgcggaaac tacaaacgat cacacaccct ctgcatgcat tgcgtaggac     1140

aaatcaggag acattggaac gactctgttc ctcaacagga ggcatttcgt gaagagtttg     1200

ttaatccttt ggatgagaag attctttatc caggaaagaa agaactgccc gatgaacgaa     1260

ctttacgtaa gaaggagtgg ctgaagagaa gaccccgaac actccctgtt gaatagaaca     1320

cgaacactgt aaatagaata aaagaaaact tggatagtag aacttcaatg tagtgtttct     1380

attgtcttac gcggctcttt agattgcaat ccccagaatg gaatcgtcca tctttctcaa     1440

cccactcaaa gataatctac cagacatacc tacgccctcc atcccagcac cacgtcgcga     1500

tcacccctaa aacttcaata attgaacacg tactgatttc ca atg ggt aag gaa        1554
                                               Met Gly Lys Glu            
                                               1                          

aag act cac gtt tcg agg ccg cga tta aat tcc aac atg gat gct gat       1602
Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp           
5                   10                  15                  20            

tta tat ggg tat aaa tgg gct cgc gat aat gtc ggg caa tca ggt gcg       1650
Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala           
                25                  30                  35                

aca atc tat cga ttg tat ggg aag ccc gat gcg cca gag ttg ttt ctg       1698
Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu           
            40                  45                  50                    

aaa cat ggc aaa ggt agc gtt gcc aat gat gtt aca gat gag atg gtc       1746
Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr Asp Glu Met Val           
        55                  60                  65                        

aga cta aac tgg ctg acg gaa ttt atg cct ctt ccg acc atc aag cat       1794
Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His           
    70                  75                  80                            

ttt atc cgt act cct gat gat gca tgg tta ctc acc act gcg atc ccc       1842
Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro           
85                  90                  95                  100           

ggc aaa aca gca ttc cag gta tta gaa gaa tat cct gat tca ggt gaa       1890
Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu           
                105                 110                 115               

aat att gtt gat gcg ctg gca gtg ttc ctg cgc cgg ttg cat tcg att       1938
Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile           
            120                 125                 130                   

cct gtt tgt aat tgt cct ttt aac agc gat cgc gta ttt cgt ctc gct       1986
Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala           
        135                 140                 145                       

cag gcg caa tca cga atg aat aac ggt ttg gtt gat gcg agt gat ttt       2034
Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe           
    150                 155                 160                           

gat gac gag cgt aat ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg       2082
Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met           
165                 170                 175                 180           

cat aag ctt ttg cca ttc tca ccg gat tca gtc gtc act cat ggt gat       2130
His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val Thr His Gly Asp           
                185                 190                 195               

ttc tca ctt gat aac ctt att ttt gac gag ggg aaa tta ata ggt tgt       2178
Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys           
            200                 205                 210                   

att gat gtt gga cga gtc gga atc gca gac cga tac cag gat ctt gcc       2226
Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala           
        215                 220                 225                       

atc cta tgg aac tgc ctc ggt gag ttt tct cct tca tta cag aaa cgg       2274
Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg           
    230                 235                 240                           

ctt ttt caa aaa tat ggt att gat aat cct gat atg aat aaa ttg cag       2322
Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln           
245                 250                 255                 260           

ttt cat ttg atg ctc gat gag ttt ttc taa tcagtactga caataaaaag         2372
Phe His Leu Met Leu Asp Glu Phe Phe                                       
                265                                                       

attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt     2432

taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg     2492

aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata     2552

ctgttcatct tagagaatgt cagtgaggtt atttctgagc taatcattac cttgcccggt     2612

acaggaatag ggtgttacct tattaattac gacggtggtc aaaacgacga aatttacccc     2672

atttttgagt tacaagacct gaatttggaa atgatgaaac aattgtacca agtcttggag     2732

gaccatgtaa gtgggcttaa tcctctcgag aagcaattcc caattgaaca cagtaaaccg     2792

ttatcagcca ctctgttctt tcacttaagg tctctttttt acatggcgaa gactcataag     2852

cgtactggaa gacattacaa cttgaaaaag attttcttgt tcactaataa cgataaacct     2912

tacaatggaa actctcagct gagagttccc ttgaagaaaa ccctggctga ttacaatgac     2972

gtagacatta ctttgattcc gtttcttctg aacaagcctt caggtgtcaa gtttgacaag     3032

acggaatact cagaaatttt gttctatgat aaagatgctt gttcgatgtc aattgaggag     3092

atccgccaac gaatttctag acataaggag atcaagcggg tttacttcac ctgtcctttg     3152

aaaatcgcaa ataacttgtg catttctgtg aaaggttatt ctatgtttta tcatgaaact     3212

ccaaggaaga tcaaatttgt cgtcaatgag ggttcaactt tcaaagatgt ggagacaaaa     3272

tctcagtttg tcgatccaac atccggaaaa gagttttcca gtgaacagct gatcaaagca     3332

tatcctctag gtgccgatgc ttacattcct ttaaactcag agcaagtcaa aacaataaat     3392

cgatttaatg atatcatcaa tatcccctct ttggaaattc taggtttcag ggatatatct     3452

aattggttgc cacagtatca gtttggcaaa gcatcgtttt tatcccctaa taa            3505


<210>  158
<211>  269
<212>  PRT
<213>  synthetic

<400>  158

Met Gly Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn 
1               5                   10                  15      


Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly 
            20                  25                  30          


Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro 
        35                  40                  45              


Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr 
    50                  55                  60                  


Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro 
65                  70                  75                  80  


Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr 
                85                  90                  95      


Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro 
            100                 105                 110         


Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg 
        115                 120                 125             


Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val 
    130                 135                 140                 


Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp 
145                 150                 155                 160 


Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val 
                165                 170                 175     


Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val 
            180                 185                 190         


Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys 
        195                 200                 205             


Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr 
    210                 215                 220                 


Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser 
225                 230                 235                 240 


Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met 
                245                 250                 255     


Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 


<210>  159
<211>  3533
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(3533)
<223>  KU70(up)-pFPR2-kanMX-terminator-KU70 construct

<220>
<221>  misc_feature
<222>  (1)..(1002)
<223>  KU70 upstream region

<220>
<221>  promoter
<222>  (1003)..(1570)
<223>  promoter pFPR2

<220>
<221>  CDS
<222>  (1571)..(2380)
<223>  kanMX

<220>
<221>  terminator
<222>  (2381)..(2583)
<223>  terminator

<220>
<221>  misc_feature
<222>  (2584)..(3533)
<223>  KU70 downstream region

<400>  159
gctcttcaga agcctaactt taatggggta aaatatcagc agtaggctga atatagagca       60

tggcaacgtt tacgtcaaca gagtttaata gtgaaaacta cgataggttc aggcctgtat      120

acccagacga gttgtatcag caattagtgg attatcatgt tggtgctaaa ggattgtgtg      180

tcgatgttgg atgtggctct ggccaggcaa cgttcaccct gaaaaagtac tttgacaagg      240

tcataggatc tgatatctca gaaaatcagt tggcagtggc taggaaaaga cagcctgctg      300

gaattgaatt taggttagga acgggcgagg atttttcttg gctaacggag acgccagatg      360

taatcaccgc cgctgaatgc ctgcactggg tagacccaca gaaattcgtt gcaaatgtcg      420

ccaattctct aagggatcat ggcaccttga gttactggct ttatacagaa ccaatttttc      480

agaacgaaag ggccaatcag gtttacaaca aattcaccta cgggtctgac tacctgggcc      540

catattggga tcctggccgt acacatttca gaaatcattt aaaggagttg aatcacatct      600

tactggatag cgagcttttt gacgaagtga aaatttctaa ttttaaacaa gaggaagggg      660

tcaaaaacgg agatatctta tacttggaaa aagagatgac aatcagtgat ttcatcaatt      720

ttgtatctag ttggccttct gtgttttcgt ggaagcagca acgaggaaag gagggtatcc      780

tagatgattt ttacaacgaa ctgaacgact gctttgaggg gggtaacatg aaagtaatat      840

ggaactccgt cctagtattt gccaggagga agcaaagggt tgtataggct ttagtactta      900

tagaggaaac ggggttacgt gcaagcgcgc atgcctgagc tttgaggggg gggactttca      960

catctcttct tctcacactt agccctaaca cagagatcaa ctacaagcgg caattcctac     1020

agaaagtgtt tgcaaagttc ctaatctcgg tttgtcacca agtcactttt ctacgaaccc     1080

tgagtttcct cacgaggaat caagttttga aatgcccgat ttgtcgcagt ttgatagttt     1140

tgatgaattt tttaatggaa tctttccaga atttaaaaga gtcaagaata tttaacttgt     1200

gagcgaacga acatgaaaac cgaaagaata gttgatgatg atgttgaagc aatgcaagta     1260

ccatataaat taggagaagt cctttgtgta ttggcgattc cttttgatgc aagtgagcgt     1320

aaatggttaa caggctgata catttactca agctagtagt gaaagccatt gcgaaatcag     1380

tttatcatag tagtggacgt cacaatggaa ctcatctctg cgaatcgttg tagaataact     1440

gtaggtaaat atgcaattct aacttctggt ctaaaatgtt cagtgacaat gcttatttct     1500

gtagtcgcga agaattgctt ctgaggagac ggagcgagcc atgtattctc gccaacttga     1560

caagaacgcc atg ggt aag gaa aag act cac gtt tcg agg ccg cga tta        1609
           Met Gly Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu            
           1               5                   10                         

aat tcc aac atg gat gct gat tta tat ggg tat aaa tgg gct cgc gat       1657
Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp           
    15                  20                  25                            

aat gtc ggg caa tca ggt gcg aca atc tat cga ttg tat ggg aag ccc       1705
Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro           
30                  35                  40                  45            

gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt agc gtt gcc aat       1753
Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn           
                50                  55                  60                

gat gtt aca gat gag atg gtc aga cta aac tgg ctg acg gaa ttt atg       1801
Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met           
            65                  70                  75                    

cct ctt ccg acc atc aag cat ttt atc cgt act cct gat gat gca tgg       1849
Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp           
        80                  85                  90                        

tta ctc acc act gcg atc ccc ggc aaa aca gca ttc cag gta tta gaa       1897
Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu           
    95                  100                 105                           

gaa tat cct gat tca ggt gaa aat att gtt gat gcg ctg gca gtg ttc       1945
Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe           
110                 115                 120                 125           

ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt cct ttt aac agc       1993
Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser           
                130                 135                 140               

gat cgc gta ttt cgt ctc gct cag gcg caa tca cga atg aat aac ggt       2041
Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly           
            145                 150                 155                   

ttg gtt gat gcg agt gat ttt gat gac gag cgt aat ggc tgg cct gtt       2089
Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val           
        160                 165                 170                       

gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca ttc tca ccg gat       2137
Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp           
    175                 180                 185                           

tca gtc gtc act cat ggt gat ttc tca ctt gat aac ctt att ttt gac       2185
Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp           
190                 195                 200                 205           

gag ggg aaa tta ata ggt tgt att gat gtt gga cga gtc gga atc gca       2233
Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala           
                210                 215                 220               

gac cga tac cag gat ctt gcc atc cta tgg aac tgc ctc ggt gag ttt       2281
Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe           
            225                 230                 235                   

tct cct tca tta cag aaa cgg ctt ttt caa aaa tat ggt att gat aat       2329
Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn           
        240                 245                 250                       

cct gat atg aat aaa ttg cag ttt cat ttg atg ctc gat gag ttt ttc       2377
Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe           
    255                 260                 265                           

taa tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta            2430

tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt     2490

tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc     2550

gtcaatcgta tgtgaatgct ggtcgctata ctgttcatct tagagaatgt cagtgaggtt     2610

atttctgagc taatcattac cttgcccggt acaggaatag ggtgttacct tattaattac     2670

gacggtggtc aaaacgacga aatttacccc atttttgagt tacaagacct gaatttggaa     2730

atgatgaaac aattgtacca agtcttggag gaccatgtaa gtgggcttaa tcctctcgag     2790

aagcaattcc caattgaaca cagtaaaccg ttatcagcca ctctgttctt tcacttaagg     2850

tctctttttt acatggcgaa gactcataag cgtactggaa gacattacaa cttgaaaaag     2910

attttcttgt tcactaataa cgataaacct tacaatggaa actctcagct gagagttccc     2970

ttgaagaaaa ccctggctga ttacaatgac gtagacatta ctttgattcc gtttcttctg     3030

aacaagcctt caggtgtcaa gtttgacaag acggaatact cagaaatttt gttctatgat     3090

aaagatgctt gttcgatgtc aattgaggag atccgccaac gaatttctag acataaggag     3150

atcaagcggg tttacttcac ctgtcctttg aaaatcgcaa ataacttgtg catttctgtg     3210

aaaggttatt ctatgtttta tcatgaaact ccaaggaaga tcaaatttgt cgtcaatgag     3270

ggttcaactt tcaaagatgt ggagacaaaa tctcagtttg tcgatccaac atccggaaaa     3330

gagttttcca gtgaacagct gatcaaagca tatcctctag gtgccgatgc ttacattcct     3390

ttaaactcag agcaagtcaa aacaataaat cgatttaatg atatcatcaa tatcccctct     3450

ttggaaattc taggtttcag ggatatatct aattggttgc cacagtatca gtttggcaaa     3510

gcatcgtttt tatcccctaa taa                                             3533


<210>  160
<211>  269
<212>  PRT
<213>  synthetic

<400>  160

Met Gly Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn 
1               5                   10                  15      


Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly 
            20                  25                  30          


Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro 
        35                  40                  45              


Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr 
    50                  55                  60                  


Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro 
65                  70                  75                  80  


Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr 
                85                  90                  95      


Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro 
            100                 105                 110         


Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg 
        115                 120                 125             


Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val 
    130                 135                 140                 


Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp 
145                 150                 155                 160 


Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val 
                165                 170                 175     


Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val 
            180                 185                 190         


Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys 
        195                 200                 205             


Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr 
    210                 215                 220                 


Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser 
225                 230                 235                 240 


Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met 
                245                 250                 255     


Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 


<210>  161
<211>  3632
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(3632)
<223>  construct KU70(up)-pRPL3-kanMX-terminator-KU70(dw)

<220>
<221>  misc_feature
<222>  (1)..(1002)
<223>  KU70 upstream region

<220>
<221>  promoter
<222>  (1003)..(1669)
<223>  pRPL3 promoter

<220>
<221>  CDS
<222>  (1670)..(2479)
<223>  kanMX

<220>
<221>  terminator
<222>  (2480)..(2682)
<223>  terminator

<220>
<221>  misc_feature
<222>  (2480)..(2682)
<223>  KU70 downstream region

<400>  161
gctcttcaga agcctaactt taatggggta aaatatcagc agtaggctga atatagagca       60

tggcaacgtt tacgtcaaca gagtttaata gtgaaaacta cgataggttc aggcctgtat      120

acccagacga gttgtatcag caattagtgg attatcatgt tggtgctaaa ggattgtgtg      180

tcgatgttgg atgtggctct ggccaggcaa cgttcaccct gaaaaagtac tttgacaagg      240

tcataggatc tgatatctca gaaaatcagt tggcagtggc taggaaaaga cagcctgctg      300

gaattgaatt taggttagga acgggcgagg atttttcttg gctaacggag acgccagatg      360

taatcaccgc cgctgaatgc ctgcactggg tagacccaca gaaattcgtt gcaaatgtcg      420

ccaattctct aagggatcat ggcaccttga gttactggct ttatacagaa ccaatttttc      480

agaacgaaag ggccaatcag gtttacaaca aattcaccta cgggtctgac tacctgggcc      540

catattggga tcctggccgt acacatttca gaaatcattt aaaggagttg aatcacatct      600

tactggatag cgagcttttt gacgaagtga aaatttctaa ttttaaacaa gaggaagggg      660

tcaaaaacgg agatatctta tacttggaaa aagagatgac aatcagtgat ttcatcaatt      720

ttgtatctag ttggccttct gtgttttcgt ggaagcagca acgaggaaag gagggtatcc      780

tagatgattt ttacaacgaa ctgaacgact gctttgaggg gggtaacatg aaagtaatat      840

ggaactccgt cctagtattt gccaggagga agcaaagggt tgtataggct ttagtactta      900

tagaggaaac ggggttacgt gcaagcgcgc atgcctgagc tttgaggggg gggactttca      960

catctcttct tctcacactt agccctaaca cagagatcaa ctccaacgac ttgaggaaat     1020

gacgattatt tcttaagata ttcatggtga ggttttccaa atgtgatctg aaatgtaatc     1080

ttggcaagtt tggttcataa aacgtcaaaa gaccacctga agataaaaaa taaagtggaa     1140

ggagaagaaa aagaggtttt tttccagatg agtctatgac taagatttct cttcaaagaa     1200

ttttttcaag gtttgagagg gatgaaagcg aggctgctgc acgattatcg atgtcattca     1260

atagggaggt agaagagaac tgagggagaa aaggctacaa ctgaacctaa aactaactga     1320

atttggaatg aagcctatta cacgagaggt gtggcgcctt tccaaagtca tcaggttcaa     1380

gttattgctt tctggagcaa taaatgatgt caatcccata cggggtctcc acccttatag     1440

aattacttat ctaccggcta ggtgttgcat cgcggaccag cccaatcggg taattttagt     1500

tgaaaaggtt tttagcgcga gtctagttat tacccggata gtctaatgct gaaaatttct     1560

cggacatttt tgatccgtac gtatatttca gtacggagaa tcatcaacaa gaatctttca     1620

atgccaccaa aaaaaaagac atttttcacc atcgcgcatt cacagtagc atg ggt aag     1678
                                                      Met Gly Lys         
                                                      1                   

gaa aag act cac gtt tcg agg ccg cga tta aat tcc aac atg gat gct       1726
Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn Met Asp Ala           
    5                   10                  15                            

gat tta tat ggg tat aaa tgg gct cgc gat aat gtc ggg caa tca ggt       1774
Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly Gln Ser Gly           
20                  25                  30                  35            

gcg aca atc tat cga ttg tat ggg aag ccc gat gcg cca gag ttg ttt       1822
Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe           
                40                  45                  50                

ctg aaa cat ggc aaa ggt agc gtt gcc aat gat gtt aca gat gag atg       1870
Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr Asp Glu Met           
            55                  60                  65                    

gtc aga cta aac tgg ctg acg gaa ttt atg cct ctt ccg acc atc aag       1918
Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro Thr Ile Lys           
        70                  75                  80                        

cat ttt atc cgt act cct gat gat gca tgg tta ctc acc act gcg atc       1966
His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile           
    85                  90                  95                            

ccc ggc aaa aca gca ttc cag gta tta gaa gaa tat cct gat tca ggt       2014
Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly           
100                 105                 110                 115           

gaa aat att gtt gat gcg ctg gca gtg ttc ctg cgc cgg ttg cat tcg       2062
Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg Leu His Ser           
                120                 125                 130               

att cct gtt tgt aat tgt cct ttt aac agc gat cgc gta ttt cgt ctc       2110
Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val Phe Arg Leu           
            135                 140                 145                   

gct cag gcg caa tca cga atg aat aac ggt ttg gtt gat gcg agt gat       2158
Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp Ala Ser Asp           
        150                 155                 160                       

ttt gat gac gag cgt aat ggc tgg cct gtt gaa caa gtc tgg aaa gaa       2206
Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val Trp Lys Glu           
    165                 170                 175                           

atg cat aag ctt ttg cca ttc tca ccg gat tca gtc gtc act cat ggt       2254
Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val Thr His Gly           
180                 185                 190                 195           

gat ttc tca ctt gat aac ctt att ttt gac gag ggg aaa tta ata ggt       2302
Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly           
                200                 205                 210               

tgt att gat gtt gga cga gtc gga atc gca gac cga tac cag gat ctt       2350
Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu           
            215                 220                 225                   

gcc atc cta tgg aac tgc ctc ggt gag ttt tct cct tca tta cag aaa       2398
Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys           
        230                 235                 240                       

cgg ctt ttt caa aaa tat ggt att gat aat cct gat atg aat aaa ttg       2446
Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met Asn Lys Leu           
    245                 250                 255                           

cag ttt cat ttg atg ctc gat gag ttt ttc taa tcagtactga caataaaaag     2499
Gln Phe His Leu Met Leu Asp Glu Phe Phe                                   
260                 265                                                   

attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt     2559

taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg     2619

aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata     2679

ctgttcatct tagagaatgt cagtgaggtt atttctgagc taatcattac cttgcccggt     2739

acaggaatag ggtgttacct tattaattac gacggtggtc aaaacgacga aatttacccc     2799

atttttgagt tacaagacct gaatttggaa atgatgaaac aattgtacca agtcttggag     2859

gaccatgtaa gtgggcttaa tcctctcgag aagcaattcc caattgaaca cagtaaaccg     2919

ttatcagcca ctctgttctt tcacttaagg tctctttttt acatggcgaa gactcataag     2979

cgtactggaa gacattacaa cttgaaaaag attttcttgt tcactaataa cgataaacct     3039

tacaatggaa actctcagct gagagttccc ttgaagaaaa ccctggctga ttacaatgac     3099

gtagacatta ctttgattcc gtttcttctg aacaagcctt caggtgtcaa gtttgacaag     3159

acggaatact cagaaatttt gttctatgat aaagatgctt gttcgatgtc aattgaggag     3219

atccgccaac gaatttctag acataaggag atcaagcggg tttacttcac ctgtcctttg     3279

aaaatcgcaa ataacttgtg catttctgtg aaaggttatt ctatgtttta tcatgaaact     3339

ccaaggaaga tcaaatttgt cgtcaatgag ggttcaactt tcaaagatgt ggagacaaaa     3399

tctcagtttg tcgatccaac atccggaaaa gagttttcca gtgaacagct gatcaaagca     3459

tatcctctag gtgccgatgc ttacattcct ttaaactcag agcaagtcaa aacaataaat     3519

cgatttaatg atatcatcaa tatcccctct ttggaaattc taggtttcag ggatatatct     3579

aattggttgc cacagtatca gtttggcaaa gcatcgtttt tatcccctaa taa            3632


<210>  162
<211>  269
<212>  PRT
<213>  synthetic

<400>  162

Met Gly Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn 
1               5                   10                  15      


Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly 
            20                  25                  30          


Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro 
        35                  40                  45              


Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr 
    50                  55                  60                  


Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro 
65                  70                  75                  80  


Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr 
                85                  90                  95      


Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro 
            100                 105                 110         


Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg 
        115                 120                 125             


Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val 
    130                 135                 140                 


Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp 
145                 150                 155                 160 


Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val 
                165                 170                 175     


Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val 
            180                 185                 190         


Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys 
        195                 200                 205             


Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr 
    210                 215                 220                 


Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser 
225                 230                 235                 240 


Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met 
                245                 250                 255     


Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 


<210>  163
<211>  3573
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(3573)
<223>  construct KU70(up)-pRPL6A-kanMX-terminator-KU70(dw)

<220>
<221>  misc_feature
<222>  (1)..(1002)
<223>  KU70 upstream region

<220>
<221>  promoter
<222>  (1003)..(1610)
<223>  promoter pRPL6A

<220>
<221>  CDS
<222>  (1611)..(2420)
<223>  kanMX

<220>
<221>  terminator
<222>  (2421)..(2623)
<223>  terminator

<220>
<221>  misc_feature
<222>  (2624)..(3573)
<223>  KU70 downstream region

<400>  163
gctcttcaga agcctaactt taatggggta aaatatcagc agtaggctga atatagagca       60

tggcaacgtt tacgtcaaca gagtttaata gtgaaaacta cgataggttc aggcctgtat      120

acccagacga gttgtatcag caattagtgg attatcatgt tggtgctaaa ggattgtgtg      180

tcgatgttgg atgtggctct ggccaggcaa cgttcaccct gaaaaagtac tttgacaagg      240

tcataggatc tgatatctca gaaaatcagt tggcagtggc taggaaaaga cagcctgctg      300

gaattgaatt taggttagga acgggcgagg atttttcttg gctaacggag acgccagatg      360

taatcaccgc cgctgaatgc ctgcactggg tagacccaca gaaattcgtt gcaaatgtcg      420

ccaattctct aagggatcat ggcaccttga gttactggct ttatacagaa ccaatttttc      480

agaacgaaag ggccaatcag gtttacaaca aattcaccta cgggtctgac tacctgggcc      540

catattggga tcctggccgt acacatttca gaaatcattt aaaggagttg aatcacatct      600

tactggatag cgagcttttt gacgaagtga aaatttctaa ttttaaacaa gaggaagggg      660

tcaaaaacgg agatatctta tacttggaaa aagagatgac aatcagtgat ttcatcaatt      720

ttgtatctag ttggccttct gtgttttcgt ggaagcagca acgaggaaag gagggtatcc      780

tagatgattt ttacaacgaa ctgaacgact gctttgaggg gggtaacatg aaagtaatat      840

ggaactccgt cctagtattt gccaggagga agcaaagggt tgtataggct ttagtactta      900

tagaggaaac ggggttacgt gcaagcgcgc atgcctgagc tttgaggggg gggactttca      960

catctcttct tctcacactt agccctaaca cagagatcaa ctcgcttcag catttcgaca     1020

gtgtaaagaa gctcatctca cttgaccaga atgcttttgc tatctgttat ttcaaagacc     1080

tagagtacct cccaattgcc atagaagctt ggaaacttta tgaaccttct tctaaagtac     1140

cggtggtatt cgctcaagtg acacaactcc ccaaatcagc tccaatcgaa tggagcgggt     1200

cagtgtatca agagacacca aactacgatg attactacgg tgattcgtca gaaaatgaag     1260

atgagtccac acagattgcc aacaacttgt cctcgctaga attacaggga caatcggtca     1320

catttcaaga cggctacgtt agcttgacat tcgccccagg cctatcagcc acagcacaca     1380

acacggtaat tttaaacaag gggcaggcaa gcaagctgga aaccattcca caggaatatt     1440

ccgtcgacat catccccgtc aacagactgt tcgatttcac agggaaagat caggacctag     1500

ccgtactcca caggctgaac atctgagatc aggcatgaga atccttacac catctatgtt     1560

gctctagggc tgtcaaccct attatgcacg tgacggtgcg actctttcaa atg ggt        1616
                                                       Met Gly            
                                                       1                  

aag gaa aag act cac gtt tcg agg ccg cga tta aat tcc aac atg gat       1664
Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn Met Asp           
        5                   10                  15                        

gct gat tta tat ggg tat aaa tgg gct cgc gat aat gtc ggg caa tca       1712
Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly Gln Ser           
    20                  25                  30                            

ggt gcg aca atc tat cga ttg tat ggg aag ccc gat gcg cca gag ttg       1760
Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro Glu Leu           
35                  40                  45                  50            

ttt ctg aaa cat ggc aaa ggt agc gtt gcc aat gat gtt aca gat gag       1808
Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr Asp Glu           
                55                  60                  65                

atg gtc aga cta aac tgg ctg acg gaa ttt atg cct ctt ccg acc atc       1856
Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro Thr Ile           
            70                  75                  80                    

aag cat ttt atc cgt act cct gat gat gca tgg tta ctc acc act gcg       1904
Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr Thr Ala           
        85                  90                  95                        

atc ccc ggc aaa aca gca ttc cag gta tta gaa gaa tat cct gat tca       1952
Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro Asp Ser           
    100                 105                 110                           

ggt gaa aat att gtt gat gcg ctg gca gtg ttc ctg cgc cgg ttg cat       2000
Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg Leu His           
115                 120                 125                 130           

tcg att cct gtt tgt aat tgt cct ttt aac agc gat cgc gta ttt cgt       2048
Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val Phe Arg           
                135                 140                 145               

ctc gct cag gcg caa tca cga atg aat aac ggt ttg gtt gat gcg agt       2096
Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp Ala Ser           
            150                 155                 160                   

gat ttt gat gac gag cgt aat ggc tgg cct gtt gaa caa gtc tgg aaa       2144
Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val Trp Lys           
        165                 170                 175                       

gaa atg cat aag ctt ttg cca ttc tca ccg gat tca gtc gtc act cat       2192
Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val Thr His           
    180                 185                 190                           

ggt gat ttc tca ctt gat aac ctt att ttt gac gag ggg aaa tta ata       2240
Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys Leu Ile           
195                 200                 205                 210           

ggt tgt att gat gtt gga cga gtc gga atc gca gac cga tac cag gat       2288
Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr Gln Asp           
                215                 220                 225               

ctt gcc atc cta tgg aac tgc ctc ggt gag ttt tct cct tca tta cag       2336
Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser Leu Gln           
            230                 235                 240                   

aaa cgg ctt ttt caa aaa tat ggt att gat aat cct gat atg aat aaa       2384
Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met Asn Lys           
        245                 250                 255                       

ttg cag ttt cat ttg atg ctc gat gag ttt ttc taa tcagtactga            2430
Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe                               
    260                 265                                               

caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt     2490

tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg     2550

cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct     2610

ggtcgctata ctgttcatct tagagaatgt cagtgaggtt atttctgagc taatcattac     2670

cttgcccggt acaggaatag ggtgttacct tattaattac gacggtggtc aaaacgacga     2730

aatttacccc atttttgagt tacaagacct gaatttggaa atgatgaaac aattgtacca     2790

agtcttggag gaccatgtaa gtgggcttaa tcctctcgag aagcaattcc caattgaaca     2850

cagtaaaccg ttatcagcca ctctgttctt tcacttaagg tctctttttt acatggcgaa     2910

gactcataag cgtactggaa gacattacaa cttgaaaaag attttcttgt tcactaataa     2970

cgataaacct tacaatggaa actctcagct gagagttccc ttgaagaaaa ccctggctga     3030

ttacaatgac gtagacatta ctttgattcc gtttcttctg aacaagcctt caggtgtcaa     3090

gtttgacaag acggaatact cagaaatttt gttctatgat aaagatgctt gttcgatgtc     3150

aattgaggag atccgccaac gaatttctag acataaggag atcaagcggg tttacttcac     3210

ctgtcctttg aaaatcgcaa ataacttgtg catttctgtg aaaggttatt ctatgtttta     3270

tcatgaaact ccaaggaaga tcaaatttgt cgtcaatgag ggttcaactt tcaaagatgt     3330

ggagacaaaa tctcagtttg tcgatccaac atccggaaaa gagttttcca gtgaacagct     3390

gatcaaagca tatcctctag gtgccgatgc ttacattcct ttaaactcag agcaagtcaa     3450

aacaataaat cgatttaatg atatcatcaa tatcccctct ttggaaattc taggtttcag     3510

ggatatatct aattggttgc cacagtatca gtttggcaaa gcatcgtttt tatcccctaa     3570

taa                                                                   3573


<210>  164
<211>  269
<212>  PRT
<213>  synthetic

<400>  164

Met Gly Lys Glu Lys Thr His Val Ser Arg Pro Arg Leu Asn Ser Asn 
1               5                   10                  15      


Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn Val Gly 
            20                  25                  30          


Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp Ala Pro 
        35                  40                  45              


Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp Val Thr 
    50                  55                  60                  


Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro Leu Pro 
65                  70                  75                  80  


Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu Leu Thr 
                85                  90                  95      


Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu Tyr Pro 
            100                 105                 110         


Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu Arg Arg 
        115                 120                 125             


Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp Arg Val 
    130                 135                 140                 


Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu Val Asp 
145                 150                 155                 160 


Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu Gln Val 
                165                 170                 175     


Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser Val Val 
            180                 185                 190         


Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu Gly Lys 
        195                 200                 205             


Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp Arg Tyr 
    210                 215                 220                 


Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser Pro Ser 
225                 230                 235                 240 


Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro Asp Met 
                245                 250                 255     


Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 


