                         SEQUENCE LISTING

<110>  Cornell University
 
<120>  COMPOSITIONS AND METHODS FOR RAPID RNA-ADENYLATION AND RNA 
       SEQUENCING

<130>  018617.01275

<150>  62/971,214
<151>  2020-02-06

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  822
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion containign E. coli PAP1 and RtcA

<400>  1

Met His His His His His His His His His Met Lys Val Leu Ser Arg 
1               5                   10                  15      


Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro Gln Val Thr Val 
            20                  25                  30          


Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp Ile Ser Glu Asn 
        35                  40                  45              


Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly Tyr Glu Ala Trp 
    50                  55                  60                  


Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly Lys Lys Pro Lys 
65                  70                  75                  80  


Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln Val Arg Lys Leu 
                85                  90                  95      


Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg Leu Ala His Val 
            100                 105                 110         


Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe Arg Gly His His 
        115                 120                 125             


Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg Gly Gln Asn Gly 
    130                 135                 140                 


Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu Glu Asp Ala Gln 
145                 150                 155                 160 


Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser Val Ala Asp Phe 
                165                 170                 175     


Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu Lys Asp Gly Val 
            180                 185                 190         


Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg Glu Asp Pro Val 
        195                 200                 205             


Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu Gly Met Arg Ile 
    210                 215                 220                 


Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala Thr Leu Leu Asn 
225                 230                 235                 240 


Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu Lys Leu Leu Gln 
                245                 250                 255     


Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys Glu Tyr His Leu 
            260                 265                 270         


Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe Thr Glu Asn Gly 
        275                 280                 285             


Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu Lys Asn Thr Asp 
    290                 295                 300                 


Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala Phe Leu Phe Ala 
305                 310                 315                 320 


Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln Lys Ile Ala Gln 
                325                 330                 335     


Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu Ala Met Asn Asp 
            340                 345                 350         


Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro Lys Arg Leu Thr 
        355                 360                 365             


Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg Met Ser Arg Arg 
    370                 375                 380                 


Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro Lys Phe Arg Ala 
385                 390                 395                 400 


Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu Arg Asn Ala Glu 
                405                 410                 415     


Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln Val Ser Ala Pro 
            420                 425                 430         


Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu Glu Pro Ser Pro 
        435                 440                 445             


Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro Arg Arg Glu Gly 
    450                 455                 460                 


Thr Ala Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
465                 470                 475                 480 


Glu Ser His Met Met Lys Arg Met Ile Ala Leu Asp Gly Ala Gln Gly 
                485                 490                 495     


Glu Gly Gly Gly Gln Ile Leu Arg Ser Ala Leu Ser Leu Ser Met Ile 
            500                 505                 510         


Thr Gly Leu Pro Phe Thr Ile Thr Gly Ile Arg Ala Gly Arg Ala Lys 
        515                 520                 525             


Pro Gly Leu Leu Arg Gln His Leu Thr Ala Val Lys Ala Ala Ala Glu 
    530                 535                 540                 


Ile Cys Arg Ala Thr Val Glu Gly Ala Glu Leu Gly Ser Gln Arg Leu 
545                 550                 555                 560 


Leu Phe Arg Pro Gly Thr Val Arg Gly Gly Asp Tyr Arg Phe Ala Ile 
                565                 570                 575     


Gly Ser Ala Gly Ser Cys Thr Leu Val Leu Gln Thr Val Leu Pro Ala 
            580                 585                 590         


Leu Trp Phe Ala Asp Gly Pro Ser Arg Val Glu Val Ser Gly Gly Thr 
        595                 600                 605             


Asp Asn Pro Ser Ala Pro Pro Ala Asp Phe Ile Arg Arg Val Leu Glu 
    610                 615                 620                 


Pro Leu Leu Ala Lys Ile Gly Ile His Gln Gln Thr Thr Leu Leu Arg 
625                 630                 635                 640 


His Gly Phe Tyr Pro Ala Gly Gly Gly Val Val Ala Thr Glu Val Ser 
                645                 650                 655     


Pro Val Thr Ser Phe Asn Thr Leu Gln Leu Gly Glu Arg Gly Asn Ile 
            660                 665                 670         


Val Arg Leu Arg Gly Glu Val Leu Leu Ala Gly Val Pro Arg His Val 
        675                 680                 685             


Ala Glu Arg Glu Ile Ala Thr Leu Ala Ala Ser Phe Ser Leu His Glu 
    690                 695                 700                 


Gln Asn Ile His Asn Leu Pro Arg Asp Gln Gly Pro Gly Asn Thr Val 
705                 710                 715                 720 


Ser Leu Glu Val Glu Ser Glu Asn Ile Thr Glu Arg Phe Phe Val Val 
                725                 730                 735     


Gly Glu Lys Arg Val Ser Ala Glu Val Val Ala Ala Gln Leu Val Lys 
            740                 745                 750         


Glu Val Lys Arg Tyr Leu Ala Ser Pro Ala Ala Val Gly Glu Tyr Leu 
        755                 760                 765             


Ala Asp Gln Leu Val Leu Pro Met Ala Leu Ala Gly Ala Gly Glu Phe 
    770                 775                 780                 


Thr Val Ala His Pro Ser Cys His Leu Leu Thr Asn Ile Ala Val Val 
785                 790                 795                 800 


Glu Arg Phe Leu Pro Val Arg Phe Gly Leu Val Glu Ala Asp Gly Val 
                805                 810                 815     


Thr Arg Val Ser Ile Glu 
            820         


<210>  2
<211>  934
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion of yeast PAP1 and RtcA

<400>  2

Met His His His His His His His His His Met Ser Ser Gln Lys Val 
1               5                   10                  15      


Phe Gly Ile Thr Gly Pro Val Ser Thr Val Gly Ala Thr Ala Ala Glu 
            20                  25                  30          


Asn Lys Leu Asn Asp Ser Leu Ile Gln Glu Leu Lys Lys Glu Gly Ser 
        35                  40                  45              


Phe Glu Thr Glu Gln Glu Thr Ala Asn Arg Val Gln Val Leu Lys Ile 
    50                  55                  60                  


Leu Gln Glu Leu Ala Gln Arg Phe Val Tyr Glu Val Ser Lys Lys Lys 
65                  70                  75                  80  


Asn Met Ser Asp Gly Met Ala Arg Asp Ala Gly Gly Lys Ile Phe Thr 
                85                  90                  95      


Tyr Gly Ser Tyr Arg Leu Gly Val His Gly Pro Gly Ser Asp Ile Asp 
            100                 105                 110         


Thr Leu Val Val Val Pro Lys His Val Thr Arg Glu Asp Phe Phe Thr 
        115                 120                 125             


Val Phe Asp Ser Leu Leu Arg Glu Arg Lys Glu Leu Asp Glu Ile Ala 
    130                 135                 140                 


Pro Val Pro Asp Ala Phe Val Pro Ile Ile Lys Ile Lys Phe Ser Gly 
145                 150                 155                 160 


Ile Ser Ile Asp Leu Ile Cys Ala Arg Leu Asp Gln Pro Gln Val Pro 
                165                 170                 175     


Leu Ser Leu Thr Leu Ser Asp Lys Asn Leu Leu Arg Asn Leu Asp Glu 
            180                 185                 190         


Lys Asp Leu Arg Ala Leu Asn Gly Thr Arg Val Thr Asp Glu Ile Leu 
        195                 200                 205             


Glu Leu Val Pro Lys Pro Asn Val Phe Arg Ile Ala Leu Arg Ala Ile 
    210                 215                 220                 


Lys Leu Trp Ala Gln Arg Arg Ala Val Tyr Ala Asn Ile Phe Gly Phe 
225                 230                 235                 240 


Pro Gly Gly Val Ala Trp Ala Met Leu Val Ala Arg Ile Cys Gln Leu 
                245                 250                 255     


Tyr Pro Asn Ala Cys Ser Ala Val Ile Leu Asn Arg Phe Phe Ile Ile 
            260                 265                 270         


Leu Ser Glu Trp Asn Trp Pro Gln Pro Val Ile Leu Lys Pro Ile Glu 
        275                 280                 285             


Asp Gly Pro Leu Gln Val Arg Val Trp Asn Pro Lys Ile Tyr Ala Gln 
    290                 295                 300                 


Asp Arg Ser His Arg Met Pro Val Ile Thr Pro Ala Tyr Pro Ser Met 
305                 310                 315                 320 


Cys Ala Thr His Asn Ile Thr Glu Ser Thr Lys Lys Val Ile Leu Gln 
                325                 330                 335     


Glu Phe Val Arg Gly Val Gln Ile Thr Asn Asp Ile Phe Ser Asn Lys 
            340                 345                 350         


Lys Ser Trp Ala Asn Leu Phe Glu Lys Asn Asp Phe Phe Phe Arg Tyr 
        355                 360                 365             


Lys Phe Tyr Leu Glu Ile Thr Ala Tyr Thr Arg Gly Ser Asp Glu Gln 
    370                 375                 380                 


His Leu Lys Trp Ser Gly Leu Val Glu Ser Lys Val Arg Leu Leu Val 
385                 390                 395                 400 


Met Lys Leu Glu Val Leu Ala Gly Ile Lys Ile Ala His Pro Phe Thr 
                405                 410                 415     


Lys Pro Phe Glu Ser Ser Tyr Cys Cys Pro Thr Glu Asp Asp Tyr Glu 
            420                 425                 430         


Met Ile Gln Asp Lys Tyr Gly Ser His Lys Thr Glu Thr Ala Leu Asn 
        435                 440                 445             


Ala Leu Lys Leu Val Thr Asp Glu Asn Lys Glu Glu Glu Ser Ile Lys 
    450                 455                 460                 


Asp Ala Pro Lys Ala Tyr Leu Ser Thr Met Tyr Ile Gly Leu Asp Phe 
465                 470                 475                 480 


Asn Ile Glu Asn Lys Lys Glu Lys Val Asp Ile His Ile Pro Cys Thr 
                485                 490                 495     


Glu Phe Val Asn Leu Cys Arg Ser Phe Asn Glu Asp Tyr Gly Asp His 
            500                 505                 510         


Lys Val Phe Asn Leu Ala Leu Arg Phe Val Lys Gly Tyr Asp Leu Pro 
        515                 520                 525             


Asp Glu Val Phe Asp Glu Asn Glu Lys Arg Pro Ser Lys Lys Ser Lys 
    530                 535                 540                 


Arg Lys Asn Leu Asp Ala Arg His Glu Thr Val Lys Arg Ser Lys Ser 
545                 550                 555                 560 


Asp Ala Ala Ser Gly Asp Asn Ile Asn Gly Thr Thr Ala Ala Val Asp 
                565                 570                 575     


Val Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
            580                 585                 590         


Glu Ser His Met Met Lys Arg Met Ile Ala Leu Asp Gly Ala Gln Gly 
        595                 600                 605             


Glu Gly Gly Gly Gln Ile Leu Arg Ser Ala Leu Ser Leu Ser Met Ile 
    610                 615                 620                 


Thr Gly Leu Pro Phe Thr Ile Thr Gly Ile Arg Ala Gly Arg Ala Lys 
625                 630                 635                 640 


Pro Gly Leu Leu Arg Gln His Leu Thr Ala Val Lys Ala Ala Ala Glu 
                645                 650                 655     


Ile Cys Arg Ala Thr Val Glu Gly Ala Glu Leu Gly Ser Gln Arg Leu 
            660                 665                 670         


Leu Phe Arg Pro Gly Thr Val Arg Gly Gly Asp Tyr Arg Phe Ala Ile 
        675                 680                 685             


Gly Ser Ala Gly Ser Cys Thr Leu Val Leu Gln Thr Val Leu Pro Ala 
    690                 695                 700                 


Leu Trp Phe Ala Asp Gly Pro Ser Arg Val Glu Val Ser Gly Gly Thr 
705                 710                 715                 720 


Asp Asn Pro Ser Ala Pro Pro Ala Asp Phe Ile Arg Arg Val Leu Glu 
                725                 730                 735     


Pro Leu Leu Ala Lys Ile Gly Ile His Gln Gln Thr Thr Leu Leu Arg 
            740                 745                 750         


His Gly Phe Tyr Pro Ala Gly Gly Gly Val Val Ala Thr Glu Val Ser 
        755                 760                 765             


Pro Val Thr Ser Phe Asn Thr Leu Gln Leu Gly Glu Arg Gly Asn Ile 
    770                 775                 780                 


Val Arg Leu Arg Gly Glu Val Leu Leu Ala Gly Val Pro Arg His Val 
785                 790                 795                 800 


Ala Glu Arg Glu Ile Ala Thr Leu Ala Ala Ser Phe Ser Leu His Glu 
                805                 810                 815     


Gln Asn Ile His Asn Leu Pro Arg Asp Gln Gly Pro Gly Asn Thr Val 
            820                 825                 830         


Ser Leu Glu Val Glu Ser Glu Asn Ile Thr Glu Arg Phe Phe Val Val 
        835                 840                 845             


Gly Glu Lys Arg Val Ser Ala Glu Val Val Ala Ala Gln Leu Val Lys 
    850                 855                 860                 


Glu Val Lys Arg Tyr Leu Ala Ser Pro Ala Ala Val Gly Glu Tyr Leu 
865                 870                 875                 880 


Ala Asp Gln Leu Val Leu Pro Met Ala Leu Ala Gly Ala Gly Glu Phe 
                885                 890                 895     


Thr Val Ala His Pro Ser Cys His Leu Leu Thr Asn Ile Ala Val Val 
            900                 905                 910         


Glu Arg Phe Leu Pro Val Arg Phe Gly Leu Val Glu Ala Asp Gly Val 
        915                 920                 925             


Thr Arg Val Ser Ile Glu 
    930                 


<210>  3
<211>  2469
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding fusion protein

<400>  3
atgcatcatc atcatcatca tcatcatcat atgaaagtgc tgagccgcga agaaagcgaa       60

gcggaacagg cggtggcgcg cccgcaggtg accgtgattc cgcgcgaaca gcatgcgatt      120

agccgcaaag atattagcga aaacgcgctg aaagtgatgt atcgcctgaa caaagcgggc      180

tatgaagcgt ggctggtggg cggcggcgtg cgcgatctgc tgctgggcaa aaaaccgaaa      240

gattttgatg tgaccaccaa cgcgaccccg gaacaggtgc gcaaactgtt tcgcaactgc      300

cgcctggtgg gccgccgctt tcgcctggcg catgtgatgt ttggcccgga aattattgaa      360

gtggcgacct ttcgcggcca tcatgaaggc aacgtgagcg atcgcaccac cagccagcgc      420

ggccagaacg gcatgctgct gcgcgataac atttttggca gcattgaaga agatgcgcag      480

cgccgcgatt ttaccattaa cagcctgtat tatagcgtgg cggattttac cgtgcgcgat      540

tatgtgggcg gcatgaaaga tctgaaagat ggcgtgattc gcctgattgg caacccggaa      600

acccgctatc gcgaagatcc ggtgcgcatg ctgcgcgcgg tgcgctttgc ggcgaaactg      660

ggcatgcgca ttagcccgga aaccgcggaa ccgattccgc gcctggcgac cctgctgaac      720

gatattccgc cggcgcgcct gtttgaagaa agcctgaaac tgctgcaggc gggctatggc      780

tatgaaacct ataaactgct gtgcgaatat catctgtttc agccgctgtt tccgaccatt      840

acccgctatt ttaccgaaaa cggcgatagc ccgatggaac gcattattga acaggtgctg      900

aaaaacaccg atacccgcat tcataacgat atgcgcgtga acccggcgtt tctgtttgcg      960

gcgatgtttt ggtatccgct gctggaaacc gcgcagaaaa ttgcgcagga aagcggcctg     1020

acctatcatg atgcgtttgc gctggcgatg aacgatgtgc tggatgaagc gtgccgcagc     1080

ctggcgattc cgaaacgcct gaccaccctg acccgcgata tttggcagct gcagctgcgc     1140

atgagccgcc gccagggcaa acgcgcgtgg aaactgctgg aacatccgaa atttcgcgcg     1200

gcgtatgatc tgctggcgct gcgcgcggaa gtggaacgca acgcggaact gcagcgcctg     1260

gtgaaatggt ggggcgaatt tcaggtgagc gcgccgccgg atcagaaagg catgctgaac     1320

gaactggatg aagaaccgag cccgcgccgc cgcacccgcc gcccgcgcaa acgcgcgccg     1380

cgccgcgaag gcaccgcgag cggcagcgag actcccggga cctcagagtc cgccacaccc     1440

gaaagtcata tgatgaaaag gatgattgcg ctggatggcg cacagggcga aggcggcggg     1500

cagatcctgc gctcggcgct gagcctgtcg atgataaccg gcctgccatt taccatcacc     1560

ggcattcgtg ccgggcgggc aaaaccggga ctgttgcgcc agcatctgac cgcggtaaaa     1620

gcggctgcgg aaatttgtag ggcaacggtg gaaggtgcgg agctgggatc gcagcgtctg     1680

ctcttccggc ccggcaccgt gcgcggcggc gattaccgct ttgctatcgg tagcgccgga     1740

agttgtacgc tggtgctgca aacggtgctg cccgcgctgt ggtttgccga tggaccttcg     1800

cgtgttgaag tgagcggagg caccgataac ccgtcggccc cgcctgcgga ttttatccgc     1860

cgggtgctgg agccgctgct ggcgaaaata ggaattcatc agcaaaccac gctgttacgt     1920

cacggttttt atcctgccgg aggcggcgtg gtggcaacgg aagtctcgcc ggtgacatcg     1980

tttaacacct tgcaacttgg cgagcgcggg aacattgtgc ggctgcgtgg tgaggtgtta     2040

ttagctggcg taccgcgaca tgttgctgag cgtgaaatcg ctacgctggc ggcaagtttt     2100

tccctgcatg agcagaatat tcataacctg ccgcgtgacc aggggccggg taataccgtt     2160

tcgcttgaag tcgaaagtga aaatatcacc gaacgctttt ttgtcgtcgg tgaaaagcgc     2220

gtcagcgccg aggtggtcgc ggcacagttg gtgaaagagg tgaaacgcta cctggcaagc     2280

ccggcggcgg tgggggaata tctcgccgac cagttggtgc taccgatggc gctggcgggc     2340

gcgggagaat ttacggtcgc ccatccctca tgccatctgc tgaccaatat cgcggtggtg     2400

gagcgtttct tgccagtgcg gtttggtctg gtggaggctg atggcgtaac gcgggtgagc     2460

attgaataa                                                             2469


<210>  4
<211>  2805
<212>  DNA
<213>  artificial sequenc

<220>
<223>  coding sequence for fusion protein

<400>  4
atgcatcatc atcatcatca tcatcatcat atgagctctc aaaaggtttt tggtattact       60

ggacctgttt ccaccgtggg cgccacagca gcagaaaata aattaaatga tagtttaatc      120

caagaactga aaaaggaagg atcgttcgaa acagagcaag aaactgccaa tagggtacaa      180

gtgttgaaaa tattgcagga attggcacaa agatttgttt atgaagtatc gaagaagaaa      240

aatatgtcag acgggatggc aagggatgct ggtgggaaga tttttacgta tgggtcctat      300

agactaggag tccatgggcc tggtagtgat atcgatactt tggtagttgt tccaaaacat      360

gtaactcggg aagatttttt tacggtattt gattcactac tgagagagag gaaggaactg      420

gatgaaattg cacctgtacc tgatgcgttt gtcccgatta tcaagataaa gttcagtggt      480

atttctatcg atttaatctg tgcacgtcta gaccaacctc aagtgccttt atccttgact      540

ttatcagata aaaatctact gcgaaatcta gacgagaagg acttgagagc tttgaatggt      600

accagagtaa cagatgagat attagaactg gtaccaaagc cgaatgtttt cagaatcgct      660

ttaagagcta ttaagctatg ggcccaaaga agggctgttt atgctaatat ttttggtttt      720

cctggtggtg tggcttgggc catgctagtg gctagaattt gtcaactata ccctaacgcc      780

tgtagcgcag ttatattgaa cagatttttc atcattttgt cggaatggaa ttggccacaa      840

cctgttatct tgaaaccaat tgaggatggc ccgttacaag ttcgtgtatg gaatccaaag      900

atatatgccc aagacaggtc tcatagaatg cccgtcatta caccagctta tccatcaatg      960

tgtgctaccc ataacatcac ggaatctact aaaaaagtca ttttacagga attcgtaaga     1020

ggcgttcaaa ttacgaatga tattttttcc aataagaagt cctgggccaa tttattcgaa     1080

aaaaacgatt ttttctttcg atacaagttc tatttagaaa ttactgcata tacaaggggc     1140

agtgacgagc agcatttaaa atggagtggt cttgttgaaa gtaaggtaag gcttctagtt     1200

atgaaactgg aggtgttagc tggaataaaa attgcacatc ctttcaccaa accctttgaa     1260

agtagttatt gttgtccaac cgaggatgac tatgaaatga ttcaagacaa atacggtagt     1320

cataaaactg agacagcact gaacgccctt aaactggtaa cagatgaaaa taaagaggaa     1380

gaaagtatta aagatgcacc aaaggcatat ttaagcacca tgtacatagg ccttgacttt     1440

aatattgaaa acaaaaagga aaaagttgac attcacattc cctgcactga atttgtgaat     1500

ttatgtcgaa gtttcaatga ggattatggt gaccacaaag tattcaatct agccctccgc     1560

ttcgtaaagg gttacgattt gccagatgaa gttttcgatg aaaatgaaaa gagaccatca     1620

aagaagagta aaaggaagaa tttagatgct agacatgaaa ccgtgaagag atctaaatca     1680

gatgctgctt caggtgacaa catcaatggc acaaccgcag ctgttgacgt aaacagcggc     1740

agcgagactc ccgggacctc agagtccgcc acacccgaaa gtcatatgat gaaaaggatg     1800

attgcgctgg atggcgcaca gggcgaaggc ggcgggcaga tcctgcgctc ggcgctgagc     1860

ctgtcgatga taaccggcct gccatttacc atcaccggca ttcgtgccgg gcgggcaaaa     1920

ccgggactgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca     1980

acggtggaag gtgcggagct gggatcgcag cgtctgctct tccggcccgg caccgtgcgc     2040

ggcggcgatt accgctttgc tatcggtagc gccggaagtt gtacgctggt gctgcaaacg     2100

gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc     2160

gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg     2220

aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc     2280

ggcgtggtgg caacggaagt ctcgccggtg acatcgttta acaccttgca acttggcgag     2340

cgcgggaaca ttgtgcggct gcgtggtgag gtgttattag ctggcgtacc gcgacatgtt     2400

gctgagcgtg aaatcgctac gctggcggca agtttttccc tgcatgagca gaatattcat     2460

aacctgccgc gtgaccaggg gccgggtaat accgtttcgc ttgaagtcga aagtgaaaat     2520

atcaccgaac gcttttttgt cgtcggtgaa aagcgcgtca gcgccgaggt ggtcgcggca     2580

cagttggtga aagaggtgaa acgctacctg gcaagcccgg cggcggtggg ggaatatctc     2640

gccgaccagt tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat     2700

ccctcatgcc atctgctgac caatatcgcg gtggtggagc gtttcttgcc agtgcggttt     2760

ggtctggtgg aggctgatgg cgtaacgcgg gtgagcattg aataa                     2805


<210>  5
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotin primer, second to last n is  C, or G, and the final N is 
       A, C, G, or T


<220>
<221>  misc_feature
<222>  (57)..(58)
<223>  n is a, c, g, or t

<400>  5
gtgactggag ttgacgtgtg ctcttccgat ctaaaaaaaa aaaaaaaaaa aaaaaann         58


<210>  6
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  adapter sequence, final two nucleotides are rG or rC


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, t or u

<400>  6
acactctttc cctacacgac gctcttccga tctnn                                  35


<210>  7
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  7
agcgagcgac caaaggaacc ataactgatt t                                      31


<210>  8
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  8
cgaggttatc tagagtcacc a                                                 21


<210>  9
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  9
tcctagctgc ggtatccagg cg                                                22


<210>  10
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  10
agatagtcaa gttcgaccgt cttctcagc                                         29


<210>  11
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  11
gggcctcgat cagaaggact tgggcccccc acga                                   34


<210>  12
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  12
cgcttggcgc cagaagcgag agcc                                              24


<210>  13
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  13
agcgacgctc agacaggcgt agccccggga g                                      31


<210>  14
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  14
ccggctatcc gaggccaacc g                                                 21


<210>  15
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  15
tgatctgata aatgcacgca tccccc                                            26


<210>  16
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  16
gtgtcgaggg ctgactttca atagatcgca gc                                     32


<210>  17
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  17
agtagtggta tttcaccggc g                                                 21


<210>  18
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  biotinylated primer

<400>  18
tagaattacc acagttatc                                                    19


<210>  19
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  19
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  20
<211>  63
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer, nnnnnn is a any nucleotide sequenc barcode


<220>
<221>  misc_feature
<222>  (25)..(30)
<223>  n is a, c, g, or t

<400>  20
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg       60

atc                                                                     63


