                               SEQUENCE LISTING

<110> TRUSTEES OF BOSTON UNIVERSITY
 
<120> ENZYME-BASED ELECTROCHEMICAL NICOTINE BIOSENSOR

<130> 701586-093890WOPT

<140> PCT/US2021/016894
<141> 2021-02-05

<150> 62/971,012
<151> 2020-02-06

<160> 7     

<170> PatentIn version 3.5

<210> 1
<211> 1449
<212> DNA
<213> Pseudomonas putida

<400> 1
atgagtgata aaacaaaaac aaatgaaggc tttagccgca ggtcttttat cggaagcgcg       60

gcagtcgtaa cagcaggtgt tgcgggattg ggagctattg atgcggcttc ggctacgcaa      120

aaaacgaacc gagcaagcac cgtcaaaggt ggcttcgatt acgatgtggt agtagttggt      180

ggagggtttg ctggcgcgac agccgcccgt gaatgtggtt tgcagggtta tcgaacgctt      240

ttattggaag cgaggtcccg cctaggtggt cgtacgttta cctcgcgctt tgcaggtcaa      300

gaaattgaat ttggcggggc atgggtgcac tggctgcagc cgcatgtttg ggcagaaatg      360

cagcgttacg gtctgggtgt agtggaagat ccacttacta atttagataa aaccttaatc      420

atgtataacg acggaagcgt cgaaagtatt tcgcccgatg aatttggcaa aaacattcga      480

atagcttttg aaaagctttg tcacgatgcc tgggaagtat ttcctcgtcc gcatgagccg      540

atgtttactg agcgcgctcg ggaattggat aaatcttctg ttcttgatcg catcaaaact      600

ttgggcttaa gtcggctgca acaggctcaa atcaatagtt acatggcctt gtatgcaggt      660

gagacaactg acaaatttgg cctgcctggt gtacttaagt tgtttgcatg cggcggttgg      720

aactatgacg ccttcatgga cactgaaact cattatagaa ttcaaggggg cacgataggc      780

ctcattaatg caatgttgac cgatagcggt gccgaggtcc gcatgtctgt gcccgtcact      840

gctgttgagc aagtcaatgg tggcgtcaaa atcaagaccg acgacgacga aattattacc      900

gccggagtgg tcgtaatgac agttccactc aatacgtata aacatatcgg ttttacgcct      960

gccctttcta aaggtaaaca acgattcatc aaagaggggc agcttagcaa aggtgctaag     1020

ctttatgttc atgttaagca gaatctcgga cgggtttttg cgtttgcgga tgaacagcaa     1080

cctttaaact gggtccagac gcacgattac agcgacgagt tggggacaat actgtcgatc     1140

accatcgctc gcaaagaaac aattgatgtg aatgaccgag atgctgtaac tcgcgaagtt     1200

caaaaaatgt ttccgggtgt tgaggttctt ggtacagcgg cttacgactg gacagctgat     1260

ccattttcct tgggggcatg ggcggcttat ggagtaggtc aactaagtcg tctcaaagat     1320

ctacaggcgg ctgaaggacg tattttattt gcaggagctg aaaccagtaa cggttggcac     1380

gcgaatatcg atggtgctgt tgaaagtgga ctacgtgccg gtagggaggt taagcagctc     1440

ttaagctag                                                             1449


<210> 2
<211> 482
<212> PRT
<213> Pseudomonas putida

<400> 2
Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210> 3
<211> 490
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala His Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser Leu Glu His His His His His His 
                485                 490 


<210> 4
<211> 490
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Tyr Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Tyr Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser Leu Glu His His His His His His 
                485                 490 


<210> 5
<211> 1473
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
atgagtgata aaacaaaaac aaatgaaggc tttagccgca ggtcttttat cggaagcgcg       60

gcagtcgtaa cagcaggtgt tgcgggattg ggagctattg atgcggcttc ggctacgcaa      120

aaaacgaacc gagcaagcac cgtcaaaggt ggcttcgatt acgatgtggt agtagttggt      180

ggagggtttg ctggcgcgac agccgcccgt gaatgtggtt tgcagggtta tcgaacgctt      240

ttattggaag cgaggtcccg cctaggtggt cgtacgttta cctcgcgctt tgcaggtcaa      300

gaaattgaat ttggcggggc atgggtgcac tggctgcagc cgcatgtttg ggcagaaatg      360

cagcgttacg gtctgggtgt agtggaagat ccacttacta atttagataa aaccttaatc      420

atgtataacg acggaagcgt cgaaagtatt tcgcccgatg aatttggcaa aaacattcga      480

atagcttttg aaaagctttg tcacgatgcc tgggaagtat ttcctcgtcc gcatgagccg      540

atgtttactg agcgcgctcg ggaattggat aaatcttctg ttcttgatcg catcaaaact      600

ttgggcttaa gtcggctgca acaggctcaa atcaatagtt acatggcctt gtatgcaggt      660

gagacaactg acaaatttgg cctgcctggt gtacttaagt tgtttgcatg cggcggttgg      720

aactatgacg ccttcatgga cactgaaact cattatagaa ttcaaggggg cacgataggc      780

ctcattaatg caatgttgac cgatagcggt gccgaggtcc gcatgtctgt gcccgtcact      840

gctgttgagc aagtcaatgg tggcgtcaaa atcaagaccg acgacgacga aattattacc      900

gccggagtgg tcgtaatgac agttccactc aatacgtata aacatatcgg ttttacgcct      960

gccctttcta aaggtaaaca acgattcatc aaagaggggc agcttagcaa aggtgctaag     1020

ctttatgttc atgttaagca gaatctcgga cgggtttttg cgtttgcgga tgaacagcaa     1080

cctttaaact gggtccagac gcacgattac agcgacgagt tggggacaat actgtcgatc     1140

accatcgctc gcaaagaaac aattgatgtg aatgaccgag atgctgtaac tcgcgaagtt     1200

caaaaaatgt ttccgggtgt tgaggttctt ggtacagcgg cttacgactg gacagctgat     1260

ccattttcct tgggggcatg ggcggcttat ggagtaggtc aactaagtcg tctcaaagat     1320

ctacaggcgg ctgaaggacg tattttattt gcaggagctg aaaccagtaa cggttggcac     1380

gcgcatatcg atggtgctgt tgaaagtgga ctacgtgccg gtagggaggt taagcagctc     1440

ttaagcctag agcaccacca ccaccaccac tag                                  1473


<210> 6
<211> 1473
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 6
atgagtgata aaacaaaaac aaatgaaggc tttagccgca ggtcttttat cggaagcgcg       60

gcagtcgtaa cagcaggtgt tgcgggattg ggagctattg atgcggcttc ggctacgcaa      120

aaaacgaacc gagcaagcac cgtcaaaggt ggcttcgatt acgatgtggt agtagttggt      180

ggagggtttg ctggcgcgac agccgcccgt gaatgtggtt tgcagggtta tcgaacgctt      240

ttattggaag cgaggtcccg cctaggtggt cgtacgttta cctcgcgctt tgcaggtcaa      300

gaaattgaat ttggcggggc atgggtgcac tggctgcagc cgcatgtttg ggcagaaatg      360

cagcgttacg gtctgggtgt agtggaagat ccacttacta atttagataa aaccttaatc      420

atgtataacg acggaagcgt cgaaagtatt tcgcccgatg aatttggcaa aaacattcga      480

atagcttttg aaaagctttg tcacgatgcc tgggaagtat ttcctcgtcc gcatgagccg      540

atgtttactg agcgcgctcg ggaattggat aaatcttctg ttcttgatcg catcaaaact      600

ttgggcttaa gtcggctgca acaggctcaa atcaatagtt acatggcctt gtatgcaggt      660

gagacaactg acaaatttgg cctgcctggt gtacttaagt tgtttgcatg cggcggttgg      720

aactatgacg ccttcatgga cactgaaact cattatagaa ttcaaggggg cacgataggc      780

ctcattaatg caatgttgac cgatagcggt gccgaggtcc gcatgtctgt gcccgtcact      840

gctgttgagc aagtcaatgg tggcgtcaaa atcaagaccg acgacgacga aattattacc      900

gccggagtgg tcgtaatgac agttccactc aatacgtata aacatatcgg ttttacgcct      960

gccctttcta aaggtaaaca acgattcatc aaagaggggc agcttagcaa aggtgctaag     1020

ctttatgttc atgttaagca gaatctcgga cgggtttttg cgtttgcgga tgaacagcaa     1080

cctttaaact gggtccagac gcacgattac agcgacgagt tggggacaat actgtcgatc     1140

accatcgctc gcaaagaaac aattgatgtg aatgaccgag atgctgtaac tcgcgaagtt     1200

caaaaaatgt ttccgggtgt tgaggttctt ggtacagcgg cttacgactg gacagctgat     1260

ccattttcct tgggggcata cgcggcttat ggagtaggtc aactaagtcg tctcaaagat     1320

ctacaggcgg ctgaaggacg tattttattt gcaggagctg aaaccagtaa cggttggcac     1380

gcgtacatcg atggtgctgt tgaaagtgga ctacgtgccg gtagggaggt taagcagctc     1440

ttaagcctag agcaccacca ccaccaccac tag                                  1473


<210> 7
<211> 305
<212> PRT
<213> Escherichia coli

<400> 7
Met Asn Ile Arg Asp Leu Glu Tyr Leu Val Ala Leu Ala Glu His Arg 
1               5                   10                  15      


His Phe Arg Arg Ala Ala Asp Ser Cys His Val Ser Gln Pro Thr Leu 
            20                  25                  30          


Ser Gly Gln Ile Arg Lys Leu Glu Asp Glu Leu Gly Val Met Leu Leu 
        35                  40                  45              


Glu Arg Thr Ser Arg Lys Val Leu Phe Thr Gln Ala Gly Met Leu Leu 
    50                  55                  60                  


Val Asp Gln Ala Arg Thr Val Leu Arg Glu Val Lys Val Leu Lys Glu 
65                  70                  75                  80  


Met Ala Ser Gln Gln Gly Glu Thr Met Ser Gly Pro Leu His Ile Gly 
                85                  90                  95      


Leu Ile Pro Thr Val Gly Pro Tyr Leu Leu Pro His Ile Ile Pro Met 
            100                 105                 110         


Leu His Gln Thr Phe Pro Lys Leu Glu Met Tyr Leu His Glu Ala Gln 
        115                 120                 125             


Thr His Gln Leu Leu Ala Gln Leu Asp Ser Gly Lys Leu Asp Cys Val 
    130                 135                 140                 


Ile Leu Ala Leu Val Lys Glu Ser Glu Ala Phe Ile Glu Val Pro Leu 
145                 150                 155                 160 


Phe Asp Glu Pro Met Leu Leu Ala Ile Tyr Glu Asp His Pro Trp Ala 
                165                 170                 175     


Asn Arg Glu Cys Val Pro Met Ala Asp Leu Ala Gly Glu Lys Leu Leu 
            180                 185                 190         


Met Leu Glu Asp Gly His Cys Leu Arg Asp Gln Ala Met Gly Phe Cys 
        195                 200                 205             


Phe Glu Ala Gly Ala Asp Glu Asp Thr His Phe Arg Ala Thr Ser Leu 
    210                 215                 220                 


Glu Thr Leu Arg Asn Met Val Ala Ala Gly Ser Gly Ile Thr Leu Leu 
225                 230                 235                 240 


Pro Ala Leu Ala Val Pro Pro Glu Arg Lys Arg Asp Gly Val Val Tyr 
                245                 250                 255     


Leu Pro Cys Ile Lys Pro Glu Pro Arg Arg Thr Ile Gly Leu Val Tyr 
            260                 265                 270         


Arg Pro Gly Ser Pro Leu Arg Ser Arg Tyr Glu Gln Leu Ala Glu Ala 
        275                 280                 285             


Ile Arg Ala Arg Met Asp Gly His Phe Asp Lys Val Leu Lys Gln Ala 
    290                 295                 300                 


Val 
305 


