                         SEQUENCE LISTING

<110>  Yale University
       Vinetz, Joseph
       Matthias, Michael
 
<120>  LEPTOSPIRAL PROTEINS AND USES THEREOF

<130>  047162-5283-00WO

<150>  US 62/971,434
<151>  2020-02-07

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  606
<212>  PRT
<213>  Leptospira interrogans

<400>  1

Ser Ser Lys Ile Glu Tyr Ser Val Ile Gln Lys Pro Thr Asp Pro Pro 
1               5                   10                  15      


Lys Asp Lys Pro Ile Lys Val Ile Val Ser Asp Gly Gly Lys Phe Cys 
            20                  25                  30          


Tyr Gly Pro Asn Phe Ser Gly Gly Glu Ser Tyr Ile Ile Ile Glu Gln 
        35                  40                  45              


Cys Trp Gln Met His Val Met Asn Ala Arg Tyr Asp Val Phe Gln Arg 
    50                  55                  60                  


Ile Ser Tyr Asn Ile Asn Asn Thr Trp Leu Cys Ile Thr Ala Pro Glu 
65                  70                  75                  80  


Lys Val Ile Lys Ala Glu Glu Thr Trp Asp Tyr Val His Leu Arg Pro 
                85                  90                  95      


Cys Thr Ile Asn Asp Pro Leu Gln Arg Trp Ile Ile Lys Asn Asn Ser 
            100                 105                 110         


Phe Trp Thr Ala Asn Gly Phe Tyr Arg Leu Lys Asp Tyr Asn Trp Tyr 
        115                 120                 125             


Gly Tyr Ile Ser Arg Asn Ser Gly Asp Arg Tyr Asn His Thr Leu Asp 
    130                 135                 140                 


Pro Ser Met Asn Asp Trp Val Asn Thr Ile Ala Thr Pro Gly Asn Ile 
145                 150                 155                 160 


Ser Ile Gln Thr Ser Ile Ala Trp Asn Leu Gln Thr Thr Glu Gly Gln 
                165                 170                 175     


Glu Arg Tyr Phe Ile Arg Trp Gly Ser Ser Asn Lys Asn Thr Thr Pro 
            180                 185                 190         


Leu Tyr Tyr Asn Pro Glu Asn Gly His Leu Ala Gln Tyr Asp Pro Ile 
        195                 200                 205             


Ser Gly Ser Leu Tyr Cys Met Tyr Ser Gln Val Asp Asn Tyr Gln Trp 
    210                 215                 220                 


Asn Trp Val Lys Trp Lys Trp Cys Ser Asp Ser Leu Glu Ser Lys Ser 
225                 230                 235                 240 


Lys Gly Asn Pro Thr Phe Trp Asn Val Phe Phe Glu Thr Asp Gln Gly 
                245                 250                 255     


Gly Met Ile Thr Asp Tyr Lys Gly Asn Ala Leu Arg Val Thr Arg Tyr 
            260                 265                 270         


Gly Ser Asn Trp Gly Val Ala Tyr Thr Ala Lys Pro Asp Phe Val Lys 
        275                 280                 285             


Thr Asp Thr Lys Asn Ser Pro Thr Ser Leu Phe Val Val Asp Lys Ser 
    290                 295                 300                 


Leu Leu Asp Trp Thr Arg Tyr Thr Ser Ser Asn Leu Gly Lys Thr Glu 
305                 310                 315                 320 


Gln Tyr Cys Pro Ala Gly Asn Lys Glu Ser Val Val His Lys Lys Ala 
                325                 330                 335     


Lys Arg Thr Leu Pro Pro Asp Phe Gln Leu Thr Glu Ala Trp Ile Arg 
            340                 345                 350         


Arg Leu Tyr Glu Ile Ala Arg Thr Asp Pro Ser Ser Arg Thr Ser Arg 
        355                 360                 365             


Gly Val Cys Gly Val Cys Met Leu Gln Ala Leu Gln Met Ile Ala Glu 
    370                 375                 380                 


Leu Gln Glu Tyr His Ser Gln Gly Pro Leu Gln Ser Gly Gly Tyr Phe 
385                 390                 395                 400 


Phe Asn Thr Ala Pro Asn Thr Asn Pro Phe Ile Ser Phe Gly Gln Arg 
                405                 410                 415     


Tyr Pro His Leu Asp Arg Leu Leu Val Asp Ile Tyr Arg Val Phe Asp 
            420                 425                 430         


His Phe Phe Asp Thr Ser His Thr Leu Gly Tyr Leu Ser Ala Met Asn 
        435                 440                 445             


Leu Leu Pro Gln Tyr Glu Trp Gly Arg Thr Arg Glu Phe Ser Thr Met 
    450                 455                 460                 


Ser Glu Ile Arg Ser His Ile Arg Ser Leu Ile Thr Ser Pro Pro Gly 
465                 470                 475                 480 


Asn Ile Trp Leu Val Leu Met Thr Met Ile Tyr Pro Asp Gly Thr Arg 
                485                 490                 495     


Gly Gly His Ala Val Pro Ile Leu Arg Thr Pro Gln Gly Leu Val Val 
            500                 505                 510         


Ile Glu Thr Thr Met Ala Thr Ala Thr Phe Glu Glu Tyr Arg Ala Ala 
        515                 520                 525             


Leu Arg Pro Thr Thr Asp Phe Glu Gln Ile Ile Arg Asn Leu Arg Gly 
    530                 535                 540                 


Pro Asn Asn Ile Leu Ile Gly Leu Ser Thr Leu Gln Leu Gly Arg Phe 
545                 550                 555                 560 


Tyr His Asn Pro Leu Asp Ser Met Ile Ser Asn Arg Asn Cys Thr Gly 
                565                 570                 575     


Glu Gly Ser Asp Arg Arg Gly Thr Gly Gly Tyr Pro Ala Ser Thr Ser 
            580                 585                 590         


Val Asn Gln Cys Ser Ser Lys Ser Ser Arg Cys Ser Leu Gln 
        595                 600                 605     


<210>  2
<211>  621
<212>  PRT
<213>  Leptospira interrogans

<400>  2

Phe Glu Tyr Gly Val Asn His Thr His Ile His Ala Leu Ser Lys Ile 
1               5                   10                  15      


Glu Tyr Ser Val Ile Gln Lys Pro Thr Asp Pro Pro Lys Asp Lys Pro 
            20                  25                  30          


Ile Lys Val Ile Val Ser Asp Gly Gly Lys Phe Cys Tyr Gly Pro Asn 
        35                  40                  45              


Phe Ser Gly Gly Glu Ser Tyr Ile Ile Ile Glu Gln Cys Trp Gln Met 
    50                  55                  60                  


His Val Met Asn Ala Arg Tyr Asp Val Phe Gln Arg Ile Ser Tyr Asn 
65                  70                  75                  80  


Ile Asn Asn Thr Trp Leu Cys Ile Thr Ala Pro Glu Lys Val Ile Lys 
                85                  90                  95      


Ala Glu Lys Asn Trp Asp Tyr Val His Leu Arg Pro Cys Thr Ile Asn 
            100                 105                 110         


Asp Pro Leu Gln Arg Trp Ile Ile Lys Asn Asn Ser Phe Trp Thr Ala 
        115                 120                 125             


Asn Gly Phe Tyr Arg Leu Lys Asp Tyr Asn Trp Tyr Gly Tyr Ile Ser 
    130                 135                 140                 


Arg Asn Ser Gly Asp Arg Tyr Asn His Thr Leu Asp Ser Ser Met Asn 
145                 150                 155                 160 


Asp Trp Val Asn Thr Ile Ala Thr Pro Gly Asn Ile Ser Ile Gln Thr 
                165                 170                 175     


Ser Ile Ala Trp Asn Leu Gln Thr Thr Glu Gly Gln Glu Arg Tyr Phe 
            180                 185                 190         


Ile Arg Trp Gly Gly Ser Asp Lys Asn Thr Thr Pro Leu Tyr Tyr Asn 
        195                 200                 205             


Pro Glu Asn Gly His Leu Ala Gln Tyr Asp Pro Ile Ser Gly Ser Leu 
    210                 215                 220                 


Tyr Cys Met Tyr Ser Gln Val Asp Asn Tyr Gln Trp Asn Trp Val Lys 
225                 230                 235                 240 


Trp Lys Trp Cys Ser Asp Leu Leu Glu Ser Lys Ser Lys Gly Asn Pro 
                245                 250                 255     


Thr Phe Trp Asn Val Phe Phe Glu Thr Asp Gln Gly Gly Met Ile Thr 
            260                 265                 270         


Asp Tyr Lys Gly Asn Ala Leu Arg Val Thr Arg Tyr Gly Ser Asn Trp 
        275                 280                 285             


Gly Ser Ala Tyr Thr Ala Lys Pro Ser Tyr Leu Glu Lys Asp Thr Thr 
    290                 295                 300                 


Asn Ser Pro Thr Ser Leu Phe Val Val Asn Lys Asp Leu Leu Asp Trp 
305                 310                 315                 320 


Thr Arg Tyr Thr Ala Ser Asn Leu Gly Lys Thr Gly Gln Tyr Cys Pro 
                325                 330                 335     


Ala Gly Lys Arg Glu Asn Ile Val His Arg Arg Val Lys Arg Glu Leu 
            340                 345                 350         


Pro Pro Asp Phe Gln Leu Thr Glu Ala Trp Ile Arg Arg Leu Tyr Glu 
        355                 360                 365             


Ile Ala Thr Ser Val Ser Ala Glu Ser Glu Thr Arg Val Ser Gly Ile 
    370                 375                 380                 


Cys Gly Pro Cys Ala Leu His Ser Phe Gln Met Leu Ala Glu Leu Leu 
385                 390                 395                 400 


Glu Tyr His Ser Arg Glu Pro Leu Gln Ser Gly Gly Tyr Phe Phe Asp 
                405                 410                 415     


Thr Ala Pro Asn Thr Asp Pro Phe Ile Ser Phe Gly Gln Arg Tyr Pro 
            420                 425                 430         


His Leu Glu Arg Leu Leu Glu Asp Ile Pro Lys Lys Tyr Ala Pro Tyr 
        435                 440                 445             


Pro His Tyr Ser Thr Gln Ser Phe Leu Ser Phe Ala Ser Ile Asp Ser 
    450                 455                 460                 


Met Leu Pro Gln Tyr Phe Trp Ser Ala Ser Thr Glu Phe Thr Asn Arg 
465                 470                 475                 480 


Asp Glu Ile Leu Ser His Ile Ser Ser Leu Ile Asn Ser Pro Ala Gly 
                485                 490                 495     


Ser Ile Trp Leu Gly Val Met Glu Gln Gln His Pro Asp Gly Thr Ile 
            500                 505                 510         


Thr Gly His Ala Ala Pro Ile Leu Arg Ile Ser Gln Gly Leu Val Val 
        515                 520                 525             


Ile Pro Thr Asn Val His Leu Trp Thr Leu Glu Glu Phe Arg Arg Phe 
    530                 535                 540                 


Leu Ile Pro Thr Thr Glu Leu Ser Gln Ile Val Ala Asn Leu Glu Gly 
545                 550                 555                 560 


Ser Asn Thr Leu Ile Arg Phe Thr Thr Ile Gln Ser Leu Gly Met Leu 
                565                 570                 575     


Thr Thr Asn Met Phe Asp Ser Met Val Ser Asn Arg Asn Cys Thr Gly 
            580                 585                 590         


Glu Gly Glu Asp Arg Arg Gly Ser Gly Glu Tyr Pro Thr Ser Thr Ser 
        595                 600                 605             


Val Asn Gln Cys Pro Ser Gly Arg Cys Ala Leu Pro Phe 
    610                 615                 620     


<210>  3
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, predicted Ricin B-like domain

<400>  3

Glu Ser Tyr Ile Ile Ile Glu Gln Cys Trp Gln Met His Val Met Asn 
1               5                   10                  15      


Ala Arg Tyr Asp Val Phe Gln Arg Ile Ser Tyr Asn Ile Asn Asn Thr 
            20                  25                  30          


Trp Leu Cys Ile Thr Ala Pro Glu Lys Val Ile Lys Ala Glu Glu Thr 
        35                  40                  45              


Trp Asp Tyr Val His Leu Arg Pro Cys Thr Ile Asn Asp Pro Leu Gln 
    50                  55                  60                  


Arg Trp Ile Ile Lys Asn Asn Ser Phe Trp Thr Ala Asn Gly Phe Tyr 
65                  70                  75                  80  


Arg Leu Lys Asp Tyr Asn Trp Tyr Gly Tyr 
                85                  90  


<210>  4
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, predicted Ricin B-like domain

<400>  4

Glu Ser Tyr Ile Ile Ile Glu Gln Cys Trp Gln Met His Val Met Asn 
1               5                   10                  15      


Ala Arg Tyr Asp Val Phe Gln Arg Ile Ser Tyr Asn Ile Asn Asn Thr 
            20                  25                  30          


Trp Leu Cys Ile Thr Ala Pro Glu Lys Val Ile Lys Ala Glu Lys Asn 
        35                  40                  45              


Trp Asp Tyr Val His Leu Arg Pro Cys Thr Ile Asn Asp Pro Leu Gln 
    50                  55                  60                  


Arg Trp Ile Ile Lys Asn Asn Ser Phe Trp Thr Ala Asn Gly Phe Tyr 
65                  70                  75                  80  


Arg Leu Lys Asp Tyr Asn Trp Tyr Gly Tyr 
                85                  90  


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, enterokinase cleavage site

<400>  5

Asp Asp Asp Asp Lys 
1               5   


<210>  6
<211>  862
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, LA_0620 (full)-mCherry Fusion protein 
       including Gly4Ser linkers and an enterokinase cleavage site

<400>  6

Ser Ser Lys Ile Glu Tyr Ser Val Ile Gln Lys Pro Thr Asp Pro Pro 
1               5                   10                  15      


Lys Asp Lys Pro Ile Lys Val Ile Val Ser Asp Gly Gly Lys Phe Cys 
            20                  25                  30          


Tyr Gly Pro Asn Phe Ser Gly Gly Glu Ser Tyr Ile Ile Ile Glu Gln 
        35                  40                  45              


Cys Trp Gln Met His Val Met Asn Ala Arg Tyr Asp Val Phe Gln Arg 
    50                  55                  60                  


Ile Ser Tyr Asn Ile Asn Asn Thr Trp Leu Cys Ile Thr Ala Pro Glu 
65                  70                  75                  80  


Lys Val Ile Lys Ala Glu Glu Thr Trp Asp Tyr Val His Leu Arg Pro 
                85                  90                  95      


Cys Thr Ile Asn Asp Pro Leu Gln Arg Trp Ile Ile Lys Asn Asn Ser 
            100                 105                 110         


Phe Trp Thr Ala Asn Gly Phe Tyr Arg Leu Lys Asp Tyr Asn Trp Tyr 
        115                 120                 125             


Gly Tyr Ile Ser Arg Asn Ser Gly Asp Arg Tyr Asn His Thr Leu Asp 
    130                 135                 140                 


Pro Ser Met Asn Asp Trp Val Asn Thr Ile Ala Thr Pro Gly Asn Ile 
145                 150                 155                 160 


Ser Ile Gln Thr Ser Ile Ala Trp Asn Leu Gln Thr Thr Glu Gly Gln 
                165                 170                 175     


Glu Arg Tyr Phe Ile Arg Trp Gly Ser Ser Asn Lys Asn Thr Thr Pro 
            180                 185                 190         


Leu Tyr Tyr Asn Pro Glu Asn Gly His Leu Ala Gln Tyr Asp Pro Ile 
        195                 200                 205             


Ser Gly Ser Leu Tyr Cys Met Tyr Ser Gln Val Asp Asn Tyr Gln Trp 
    210                 215                 220                 


Asn Trp Val Lys Trp Lys Trp Cys Ser Asp Ser Leu Glu Ser Lys Ser 
225                 230                 235                 240 


Lys Gly Asn Pro Thr Phe Trp Asn Val Phe Phe Glu Thr Asp Gln Gly 
                245                 250                 255     


Gly Met Ile Thr Asp Tyr Lys Gly Asn Ala Leu Arg Val Thr Arg Tyr 
            260                 265                 270         


Gly Ser Asn Trp Gly Val Ala Tyr Thr Ala Lys Pro Asp Phe Val Lys 
        275                 280                 285             


Thr Asp Thr Lys Asn Ser Pro Thr Ser Leu Phe Val Val Asp Lys Ser 
    290                 295                 300                 


Leu Leu Asp Trp Thr Arg Tyr Thr Ser Ser Asn Leu Gly Lys Thr Glu 
305                 310                 315                 320 


Gln Tyr Cys Pro Ala Gly Asn Lys Glu Ser Val Val His Lys Lys Ala 
                325                 330                 335     


Lys Arg Thr Leu Pro Pro Asp Phe Gln Leu Thr Glu Ala Trp Ile Arg 
            340                 345                 350         


Arg Leu Tyr Glu Ile Ala Arg Thr Asp Pro Ser Ser Arg Thr Ser Arg 
        355                 360                 365             


Gly Val Cys Gly Val Cys Met Leu Gln Ala Leu Gln Met Ile Ala Glu 
    370                 375                 380                 


Leu Gln Glu Tyr His Ser Gln Gly Pro Leu Gln Ser Gly Gly Tyr Phe 
385                 390                 395                 400 


Phe Asn Thr Ala Pro Asn Thr Asn Pro Phe Ile Ser Phe Gly Gln Arg 
                405                 410                 415     


Tyr Pro His Leu Asp Arg Leu Leu Val Asp Ile Tyr Arg Val Phe Asp 
            420                 425                 430         


His Phe Phe Asp Thr Ser His Thr Leu Gly Tyr Leu Ser Ala Met Asn 
        435                 440                 445             


Leu Leu Pro Gln Tyr Glu Trp Gly Arg Thr Arg Glu Phe Ser Thr Met 
    450                 455                 460                 


Ser Glu Ile Arg Ser His Ile Arg Ser Leu Ile Thr Ser Pro Pro Gly 
465                 470                 475                 480 


Asn Ile Trp Leu Val Leu Met Thr Met Ile Tyr Pro Asp Gly Thr Arg 
                485                 490                 495     


Gly Gly His Ala Val Pro Ile Leu Arg Thr Pro Gln Gly Leu Val Val 
            500                 505                 510         


Ile Glu Thr Thr Met Ala Thr Ala Thr Phe Glu Glu Tyr Arg Ala Ala 
        515                 520                 525             


Leu Arg Pro Thr Thr Asp Phe Glu Gln Ile Ile Arg Asn Leu Arg Gly 
    530                 535                 540                 


Pro Asn Asn Ile Leu Ile Gly Leu Ser Thr Leu Gln Leu Gly Arg Phe 
545                 550                 555                 560 


Tyr His Asn Pro Leu Asp Ser Met Ile Ser Asn Arg Asn Cys Thr Gly 
                565                 570                 575     


Glu Gly Ser Asp Arg Arg Gly Thr Gly Gly Tyr Pro Ala Ser Thr Ser 
            580                 585                 590         


Val Asn Gln Cys Ser Ser Lys Ser Ser Arg Cys Ser Leu Gln Gly Gly 
        595                 600                 605             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp Asp 
    610                 615                 620                 


Asp Lys Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys 
625                 630                 635                 640 


Glu Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His 
                645                 650                 655     


Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr 
            660                 665                 670         


Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala 
        675                 680                 685             


Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val 
    690                 695                 700                 


Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu 
705                 710                 715                 720 


Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val 
                725                 730                 735     


Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys 
            740                 745                 750         


Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln 
        755                 760                 765             


Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu 
    770                 775                 780                 


Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp 
785                 790                 795                 800 


Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys 
                805                 810                 815     


Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile 
            820                 825                 830         


Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala 
        835                 840                 845             


Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
    850                 855                 860         


<210>  7
<211>  877
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, LA_3490 (full)-mCherry Fusion protein 
       including Gly4Ser linkers and an enterokinase cleavage site

<400>  7

Phe Glu Tyr Gly Val Asn His Thr His Ile His Ala Leu Ser Lys Ile 
1               5                   10                  15      


Glu Tyr Ser Val Ile Gln Lys Pro Thr Asp Pro Pro Lys Asp Lys Pro 
            20                  25                  30          


Ile Lys Val Ile Val Ser Asp Gly Gly Lys Phe Cys Tyr Gly Pro Asn 
        35                  40                  45              


Phe Ser Gly Gly Glu Ser Tyr Ile Ile Ile Glu Gln Cys Trp Gln Met 
    50                  55                  60                  


His Val Met Asn Ala Arg Tyr Asp Val Phe Gln Arg Ile Ser Tyr Asn 
65                  70                  75                  80  


Ile Asn Asn Thr Trp Leu Cys Ile Thr Ala Pro Glu Lys Val Ile Lys 
                85                  90                  95      


Ala Glu Lys Asn Trp Asp Tyr Val His Leu Arg Pro Cys Thr Ile Asn 
            100                 105                 110         


Asp Pro Leu Gln Arg Trp Ile Ile Lys Asn Asn Ser Phe Trp Thr Ala 
        115                 120                 125             


Asn Gly Phe Tyr Arg Leu Lys Asp Tyr Asn Trp Tyr Gly Tyr Ile Ser 
    130                 135                 140                 


Arg Asn Ser Gly Asp Arg Tyr Asn His Thr Leu Asp Ser Ser Met Asn 
145                 150                 155                 160 


Asp Trp Val Asn Thr Ile Ala Thr Pro Gly Asn Ile Ser Ile Gln Thr 
                165                 170                 175     


Ser Ile Ala Trp Asn Leu Gln Thr Thr Glu Gly Gln Glu Arg Tyr Phe 
            180                 185                 190         


Ile Arg Trp Gly Gly Ser Asp Lys Asn Thr Thr Pro Leu Tyr Tyr Asn 
        195                 200                 205             


Pro Glu Asn Gly His Leu Ala Gln Tyr Asp Pro Ile Ser Gly Ser Leu 
    210                 215                 220                 


Tyr Cys Met Tyr Ser Gln Val Asp Asn Tyr Gln Trp Asn Trp Val Lys 
225                 230                 235                 240 


Trp Lys Trp Cys Ser Asp Leu Leu Glu Ser Lys Ser Lys Gly Asn Pro 
                245                 250                 255     


Thr Phe Trp Asn Val Phe Phe Glu Thr Asp Gln Gly Gly Met Ile Thr 
            260                 265                 270         


Asp Tyr Lys Gly Asn Ala Leu Arg Val Thr Arg Tyr Gly Ser Asn Trp 
        275                 280                 285             


Gly Ser Ala Tyr Thr Ala Lys Pro Ser Tyr Leu Glu Lys Asp Thr Thr 
    290                 295                 300                 


Asn Ser Pro Thr Ser Leu Phe Val Val Asn Lys Asp Leu Leu Asp Trp 
305                 310                 315                 320 


Thr Arg Tyr Thr Ala Ser Asn Leu Gly Lys Thr Gly Gln Tyr Cys Pro 
                325                 330                 335     


Ala Gly Lys Arg Glu Asn Ile Val His Arg Arg Val Lys Arg Glu Leu 
            340                 345                 350         


Pro Pro Asp Phe Gln Leu Thr Glu Ala Trp Ile Arg Arg Leu Tyr Glu 
        355                 360                 365             


Ile Ala Thr Ser Val Ser Ala Glu Ser Glu Thr Arg Val Ser Gly Ile 
    370                 375                 380                 


Cys Gly Pro Cys Ala Leu His Ser Phe Gln Met Leu Ala Glu Leu Leu 
385                 390                 395                 400 


Glu Tyr His Ser Arg Glu Pro Leu Gln Ser Gly Gly Tyr Phe Phe Asp 
                405                 410                 415     


Thr Ala Pro Asn Thr Asp Pro Phe Ile Ser Phe Gly Gln Arg Tyr Pro 
            420                 425                 430         


His Leu Glu Arg Leu Leu Glu Asp Ile Pro Lys Lys Tyr Ala Pro Tyr 
        435                 440                 445             


Pro His Tyr Ser Thr Gln Ser Phe Leu Ser Phe Ala Ser Ile Asp Ser 
    450                 455                 460                 


Met Leu Pro Gln Tyr Phe Trp Ser Ala Ser Thr Glu Phe Thr Asn Arg 
465                 470                 475                 480 


Asp Glu Ile Leu Ser His Ile Ser Ser Leu Ile Asn Ser Pro Ala Gly 
                485                 490                 495     


Ser Ile Trp Leu Gly Val Met Glu Gln Gln His Pro Asp Gly Thr Ile 
            500                 505                 510         


Thr Gly His Ala Ala Pro Ile Leu Arg Ile Ser Gln Gly Leu Val Val 
        515                 520                 525             


Ile Pro Thr Asn Val His Leu Trp Thr Leu Glu Glu Phe Arg Arg Phe 
    530                 535                 540                 


Leu Ile Pro Thr Thr Glu Leu Ser Gln Ile Val Ala Asn Leu Glu Gly 
545                 550                 555                 560 


Ser Asn Thr Leu Ile Arg Phe Thr Thr Ile Gln Ser Leu Gly Met Leu 
                565                 570                 575     


Thr Thr Asn Met Phe Asp Ser Met Val Ser Asn Arg Asn Cys Thr Gly 
            580                 585                 590         


Glu Gly Glu Asp Arg Arg Gly Ser Gly Glu Tyr Pro Thr Ser Thr Ser 
        595                 600                 605             


Val Asn Gln Cys Pro Ser Gly Arg Cys Ala Leu Pro Phe Gly Gly Gly 
    610                 615                 620                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp Asp Asp 
625                 630                 635                 640 


Lys Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu 
                645                 650                 655     


Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu 
            660                 665                 670         


Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln 
        675                 680                 685             


Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp 
    690                 695                 700                 


Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys 
705                 710                 715                 720 


His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly 
                725                 730                 735     


Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr 
            740                 745                 750         


Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val 
        755                 760                 765             


Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys 
    770                 775                 780                 


Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp 
785                 790                 795                 800 


Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly 
                805                 810                 815     


Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro 
            820                 825                 830         


Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr 
        835                 840                 845             


Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu 
    850                 855                 860                 


Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
865                 870                 875         


<210>  8
<211>  1821
<212>  DNA
<213>  Leptospira interrogans

<400>  8
tcatcaaaaa ttgaatactc ggttattcaa aaacctaccg atccaccaaa agacaaacca       60

atcaaagtaa ttgtaagcga tggaggaaag ttttgttacg gtcctaattt tagcggaggt      120

gaaagttaca ttataattga acagtgttgg caaatgcacg ttatgaatgc aagatacgac      180

gtgtttcaaa gaatttcgta taacatcaat aatacgtggt tatgtattac tgctccggag      240

aaagtaatta aagcagaaga aacctgggac tatgttcatc tcagaccttg tacgatcaat      300

gatcctctgc agagatggat tataaaaaac aattcttttt ggactgcaaa tgggttttac      360

cgattgaagg attataattg gtacggctat atctctagaa attctggtga tagatacaat      420

catactttag atccttccat gaacgattgg gtgaatacaa tagccactcc cggaaatatc      480

agcattcaaa cttctatagc ctggaatttg caaactactg agggacagga acgttatttt      540

attcgctggg gaagttcgaa taaaaataca actcctctct actacaatcc tgaaaatgga      600

catctcgctc agtatgatcc aatcagtggt tctctctact gtatgtattc tcaagtagac      660

aactatcaat ggaattgggt gaaatggaaa tggtgtagtg attcacttga aagtaaaagc      720

aagggaaatc caactttttg gaatgtcttt tttgaaactg atcaaggagg aatgattaca      780

gattataagg gaaatgcgct gagagttact agatatggaa gcaattgggg tgttgcttat      840

acggctaagc ctgattttgt caaaacggat actaagaata gtcccacttc tttatttgta      900

gttgataaaa gcttactgga ttggacacgt tatacgtctt ctaatcttgg aaagacagag      960

cagtattgtc cagctggtaa taaagaaagt gttgtacata aaaaagccaa aagaacctta     1020

ccgcccgact ttcaattaac tgaggcttgg attagaagac tttatgaaat agcaaggacg     1080

gatccaagtt cgcgtacgtc acgtggagta tgtggtgttt gtatgcttca agctcttcag     1140

atgatagcag aactccagga gtatcattct caaggacctc ttcagagtgg aggttacttc     1200

ttcaatacgg ctcctaatac aaaccctttt atctcgttcg gacaacgtta tccgcacttg     1260

gacaggttgc tagtagatat ttacagagtg tttgaccatt tctttgacac aagccatacg     1320

ttaggatact tatctgctat gaatctctta cctcagtacg aatgggggcg cactcgtgaa     1380

ttctccacta tgtctgaaat acgatcccac attagatcac tcataacttc cccacccgga     1440

aatatttggc tagtgttaat gacaatgatt tatccagatg gaacgagagg agggcatgcg     1500

gttccaattc ttagaacccc tcaaggatta gttgtaattg aaacaaccat ggcaaccgca     1560

acatttgaag aatacagagc agcgttaaga cccactacag attttgaaca gataattaga     1620

aatctgagag gacccaataa tattctaata ggactttcaa ctttacaatt aggaagattt     1680

taccacaatc cgttggactc tatgatatct aacagaaatt gcaccgggga aggaagtgat     1740

agaagaggca caggaggata tccagctagc acatcggtaa accaatgctc aagtaaaagc     1800

agccggtgct ccctgcagta a                                               1821


<210>  9
<211>  1864
<212>  DNA
<213>  Leptospira interrogans

<400>  9
cgagtatgga gtaaatcata cacatatcca cgctttatca aaaattgaat actcggttat       60

tcaaaaacct accgatccac caaaagacaa accaatcaaa gtaattgtaa gcgatggagg      120

aaagttttgt tacggtccta attttagcgg aggtgaaagt tatattataa ttgaacagtg      180

ttggcaaatg cacgttatga atgcaagata cgacgtgttt caaagaattt cgtataacat      240

caataatacg tggttatgta ttactgctcc ggagaaagta attaaagcag agaaaaactg      300

ggactatgtt catctcagac cttgtacgat caatgatcct ctgcaaagat ggattataaa      360

aaacaattct ttttggactg caaatgggtt ttaccgattg aaggattata attggtacgg      420

ttatatctct agaaattctg gtgatagata caatcatact ttagattctt ccatgaacga      480

ttgggtgaat acaatagcta ctcccggaaa tattagcatt caaacttcta tagcctggaa      540

tttgcaaact actgagggac aggaacgtta ttttattcgc tggggtggtt cagataaaaa      600

tacaactcct ctctactaca atcctgaaaa tggacatctc gctcagtatg atccaatcag      660

tggttctctc tattgtatgt attctcaggt agacaactat caatggaatt gggtgaaatg      720

gaaatggtgt agtgatttac ttgaaagtaa aagcaaggga aatccaactt tttggaatgt      780

cttttttgaa actgatcaag gaggaatgat tacagattat aagggaaatg cgctgagagt      840

tactagatat ggatccaatt ggggctctgc ctatacagcc aaaccttctt atttagaaaa      900

ggacactacc aatagcccaa cttctctgtt tgttgttaat aaagatttat tggattggac      960

acgttataca gcaagtaacc ttggtaaaac aggacaatat tgtccggctg gcaaaagaga     1020

aaatattgta catagaagag tcaaaagaga attaccaccc gactttcaat taactgaggc     1080

ttggatccga agactttatg aaatagcaac ttcagtttcc gctgaatccg agactcgagt     1140

cagtggaatc tgtggccctt gtgctcttca tagctttcag atgttggcag agcttctgga     1200

gtatcattct cgagaacctc ttcagagtgg aggttacttt tttgatacag ctccaaatac     1260

agatcctttt atatcgtttg gtcaacgtta tccgcatttg gaaagactgt tagaagatat     1320

acctaaaaag tatgctcctt atcctcacta ttctacacaa agtttcttat catttgcatc     1380

tattgattct atgctgcccc aatatttttg gtctgcttct actgaattta cgaatcggga     1440

tgagattctt tctcacatta gctcacttat taattcccct gctggaagca tttggttagg     1500

agtcatggag caacaacatc ctgatggaac cataacagga cacgcagctc caattcttag     1560

aatctctcag ggattagtag taattccaac taacgtgcat ttgtggacat tagaagaatt     1620

cagaagattt ttaataccca ccacagagct gtctcaaata gttgcgaatt tggaaggatc     1680

aaatacactg ataaggttta caactataca atcattggga atgctcacaa caaatatgtt     1740

tgattccatg gtctctaaca gaaattgtac gggagaggga gaagacagaa gaggttctgg     1800

agaatatcca acaagtacat ccgtaaatca atgtccaagc ggcagatgtg cattaccatt     1860

ttaa                                                                  1864


<210>  10
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, predicted Ricin B domain LA_0620, short

<400>  10
gaaagttaca ttataattga acagtgttgg caaatgcacg ttatgaatgc aagatacgac       60

gtgtttcaaa gaatttcgta taacatcaat aatacgtggt tatgtattac tgctccggag      120

aaagtaatta aagcagaaga aacctgggac tatgttcatc tcagaccttg tacgatcaat      180

gatcctctgc agagatggat tataaaaaac aattcttttt ggactgcaaa tgggttttac      240

cgattgaagg attataattg gtacggctat                                       270


<210>  11
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, predicted Ricin B domain LA_3490, short

<400>  11
gaaagttata ttataattga acagtgttgg caaatgcacg ttatgaatgc aagatacgac       60

gtgtttcaaa gaatttcgta taacatcaat aatacgtggt tatgtattac tgctccggag      120

aaagtaatta aagcagagaa aaactgggac tatgttcatc tcagaccttg tacgatcaat      180

gatcctctgc aaagatggat tataaaaaac aattcttttt ggactgcaaa tgggttttac      240

cgattgaagg attataattg gtacggttat                                       270


