               SEQUENCE LISTING

<110> Xbrane Biopharma AB
      CloneOpt AB

<120> Controlling signal peptide performance

<130> 2020Q101

<160> 62

<170> BiSSAP 1.3.6

<210> 1
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region - short sequence - A14

<400> 1
cgtattatga aaatt                                                     15


<210> 2
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region - short sequence - A2

<400> 2
tcatggaatg aagatc                                                    16


<210> 3
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region - short short sequence -
      A6

<400> 3
ttagtggatg aaaata                                                    16


<210> 4
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region - short sequence - B16

<400> 4
gttcgtatga agaag                                                     15


<210> 5
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region - short sequence - B6

<400> 5
aaggaaatga agaag                                                     15


<210> 6
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region - short sequence - B7

<400> 6
aattctatga aaaag                                                     15


<210> 7
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - syn_evolved - Randomized region - short sequence - C5

<400> 7
taacgtatga agcaa                                                     15


<210> 8
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - syn_evolved - Randomized region - short sequence - C3

<400> 8
attatgatga agcaa                                                     15


<210> 9
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region - short sequence - D15

<400> 9
cgtaggatga aaaag                                                     15


<210> 10
<211> 14
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region - short sequence - D2

<400> 10
ggtggatgaa gaaa                                                      14


<210> 11
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region - short sequence - D11

<400> 11
ggctccatga aaaag                                                     15


<210> 12
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region - short sequence - E5

<400> 12
atcaggatga agtat                                                     15


<210> 13
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region - short sequence - E12

<400> 13
tcaagtatga aatat                                                     15


<210> 14
<211> 15
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region - short sequence - E13

<400> 14
ctgtttatga aatat                                                     15


<210> 15
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region - full sequence - A14

<400> 15
gtttaacttt aagaaggaga tcgtattatg aaaattaaaa caggtgcacg c              51


<210> 16
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region – full sequence - A2

<400> 16
gtttaacttt aagaaggaga tcatggaatg aagatcaaaa caggtgcacg c              51


<210> 17
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - syn_evolved - Randomized region – full sequence - A6

<400> 17
gtttaacttt aagaaggaga ttagtggatg aaaataaaaa caggtgcacg c              51


<210> 18
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region – full sequence - B16

<400> 18
gtttaacttt aagaaggaga tgttcgtatg aagaagacag ctatcgcgat t              51


<210> 19
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region – full sequence - B6

<400> 19
gtttaacttt aagaaggaga taaggaaatg aagaagacag ctatcgcgat t              51


<210> 20
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - syn_evolved - Randomized region – full sequence - B7

<400> 20
gtttaacttt aagaaggaga taattctatg aaaaagacag ctatcgcgat t              51


<210> 21
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - syn_evolved - Randomized region – full sequence - C5

<400> 21
gtttaacttt aagaaggaga ttaacgtatg aagcaaagca ctattgcact g              51


<210> 22
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - syn_evolved - Randomized region – full sequence - C3

<400> 22
gtttaacttt aagaaggaga tattatgatg aagcaaagca ctattgcact g              51


<210> 23
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region – full sequence - D15

<400> 23
gtttaacttt aagaaggaga tcgtaggatg aaaaagattt ggctggcgct g              51


<210> 24
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region – full sequence - D2

<400> 24
gtttaacttt aagaaggaga tcggtggatg aagaaaattt ggctggcgct g              51


<210> 25
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - syn_evolved - Randomized region – full sequence - D11

<400> 25
gtttaacttt aagaaggaga tggctccatg aaaaagattt ggctggcgct g              51


<210> 26
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region – full sequence - E5

<400> 26
gtttaacttt aagaaggaga tatcaggatg aagtatctgc tgccgaccgc t              51


<210> 27
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region – full sequence - E12

<400> 27
gtttaacttt aagaaggaga ttcaagtatg aaatatctgc tgccgaccgc t              51


<210> 28
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - syn_evolved - Randomized region – full sequence - E13

<400> 28
gtttaacttt aagaaggaga tctgtttatg aaatatctgc tgccgaccgc t              51


<210> 29
<211> 26
<212> PRT
<213> Escherichia coli


<220> 
<223> MalE signal peptide

<400> 29
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      
Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      

<210> 30
<211> 21
<212> PRT
<213> Escherichia coli


<220> 
<223> OmpA signal peptide

<400> 30
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      
Thr Val Ala Gln Ala 
            20      

<210> 31
<211> 21
<212> PRT
<213> Escherichia coli


<220> 
<223> PhoA signal peptide

<400> 31
Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      
Pro Val Thr Lys Ala 
            20      

<210> 32
<211> 19
<212> PRT
<213> Escherichia coli


<220> 
<223> DsbA signal peptide

<400> 32
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      
Ala Ser Ala 
            

<210> 33
<211> 22
<212> PRT
<213> Erwinia carotovora


<220> 
<223> PelB signal peptide

<400> 33
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      
Ala Gln Pro Ala Met Ala 
            20          

<210> 34
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - SP - DNA sequence - A14

<400> 34
atgaaaatta aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt     60

tccgcctcgg ctctcgccca c                                               81


<210> 35
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - SP - DNA sequence - A2

<400> 35
atgaagatca aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt     60

tccgcctcgg ctctcgccca c                                               81


<210> 36
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - SP - DNA sequence - A6

<400> 36
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt     60

tccgcctcgg ctctcgccca c                                               81


<210> 37
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - SP - DNA sequence - B16

<400> 37
atgaagaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag     60

gcccac                                                                66


<210> 38
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - SP - DNA sequence - B6

<400> 38
atgaagaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag     60

gcccac                                                                66


<210> 39
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - SP - DNA sequence - B7

<400> 39
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag     60

gcccac                                                                66


<210> 40
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - SP - DNA sequence - C5

<400> 40
atgaagcaaa gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa     60

gcccac                                                                66


<210> 41
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - SP - DNA sequence - C3

<400> 41
atgaagcaaa gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa     60

gcccac                                                                66


<210> 42
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - SP - DNA sequence - D15

<400> 42
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcgcac     60


<210> 43
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - SP - DNA sequence - D2

<400> 43
atgaagaaaa tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcgcac     60


<210> 44
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - SP - DNA sequence - D11

<400> 44
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcgcac     60


<210> 45
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - SP - DNA sequence - E5

<400> 45
atgaagtatc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg     60

atggcccac                                                             69


<210> 46
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - SP - DNA sequence - E12

<400> 46
atgaaatatc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg     60

atggcccac                                                             69


<210> 47
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - SP - DNA sequence - E13

<400> 47
atgaaatatc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg     60

atggcccac                                                             69


<210> 48
<211> 15
<212> DNA
<213> Escherichia coli


<220> 
<223> MalE - unevolved - Short sequence

<400> 48
ataccgatga aaata                                                     15


<210> 49
<211> 51
<212> DNA
<213> Escherichia coli


<220> 
<223> MalE- unevolved - Full sequence

<400> 49
gtttaacttt aagaaggaga tataccgatg aaaataaaaa caggtgcacg c              51


<210> 50
<211> 15
<212> DNA
<213> Escherichia coli


<220> 
<223> OmpA - unevolved - Short sequence

<400> 50
ataccgatga aaaag                                                     15


<210> 51
<211> 51
<212> DNA
<213> Escherichia coli


<220> 
<223> OmpA - unevolved - Full sequence

<400> 51
gtttaacttt aagaaggaga tataccgatg aaaaagacag ctatcgcgat t              51


<210> 52
<211> 15
<212> DNA
<213> Escherichia coli


<220> 
<223> PhoA - unevolved - Short sequence

<400> 52
ataccgatga aacaa                                                     15


<210> 53
<211> 51
<212> DNA
<213> Escherichia coli


<220> 
<223> PhoA - unevolved - Full sequence

<400> 53
gtttaacttt aagaaggaga tataccgatg aaacaaagca ctattgcact g              51


<210> 54
<211> 15
<212> DNA
<213> Escherichia coli


<220> 
<223> DsbA - unevolved - Short sequence

<400> 54
ataccgatga aaaag                                                     15


<210> 55
<211> 51
<212> DNA
<213> Escherichia coli


<220> 
<223> DsbA - unevolved - Full sequence

<400> 55
gtttaacttt aagaaggaga tataccgatg aaaaagattt ggctggcgct g              51


<210> 56
<211> 15
<212> DNA
<213> Erwinia carotovora


<220> 
<223> PelB - unevolved - Short sequence

<400> 56
ataccgatga aatac                                                     15


<210> 57
<211> 51
<212> DNA
<213> Erwinia carotovora


<220> 
<223> PelB - unevolved - Full sequence

<400> 57
gtttaacttt aagaaggaga tataccgatg aaatacctgc tgccgaccgc t              51


<210> 58
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> MalE - SP - unevolved - DNA sequence

<400> 58
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt     60

tccgcctcgg ctctcgccca c                                               81


<210> 59
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> OmpA - SP - unevolved - DNA sequence

<400> 59
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag     60

gcccac                                                                66


<210> 60
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> PhoA - SP - unevolved - DNA sequence

<400> 60
atgaaacaaa gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa     60

gcccac                                                                66


<210> 61
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> DsbA - SP - unevolved - DNA sequence

<400> 61
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcgcac     60


<210> 62
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> PelB - SP - unevolved - DNA sequence

<400> 62
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg     60

atggcccac                                                             69


