<110> APPLICANTS: Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food; Wieden, Hans-Joachim; Smith, Dustin D.; Girodat, Dylan; Roberts, Luc Arden Hamilton

<120> TITLE OF INVENTION: Improved Processes for Rationally-Designing and Producing Biomolecules

<130> FILE REFERENCE: 128808.002

<150> EARLIER PATENT APPLICATION: US 62/969,317
<151> EARLIER APPLICATION FILING DATE: 2020-02-03

<160> NUMBER OF SEQ ID NOS: 3

<210> SEQ ID NO: 1
<211> LENGTH: 416
<212> TYPE: PRT
<213> ORGANISM: Streptococcus pneumoniae

<220> FEATURE:
<223> OTHER INFORMATION: Unmodified MalX

<300> PUBLICATION INFORMATION:

<400> SEQUENCE: 1

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
  1               5                  10                  15   
    
Arg Gly Ser His Met Ala Ser Asp Lys Pro Ala Asp Ser Gly Ser Ser 
             20                  25                  30      

Glu Val Lys Glu Leu Thr Val Tyr Val Asp Glu Gly Tyr Lys Ser Tyr 
         35                  40                  45      

Ile Glu Glu Val Ala Lys Ala Tyr Glu Lys Glu Ala Gly Val Lys Val 
     50                  55                  60         

Thr Leu Lys Thr Gly Asp Ala Leu Gly Gly Leu Asp Lys Leu Ser Leu
 65                  70                  75                  80

Asp Asn Gln Asn Gly Asn Val Pro Asp Val Met Met Ala Pro Tyr Asp 
                 85                  90                  95

Arg Val Gly Ser Leu Gly Ser Asp Gly Gln Leu Ser Glu Val Lys Leu 
            100                 105                 110   

Ser Asp Gly Ala Lys Thr Asp Asp Thr Thr Lys Ser Leu Val Thr Ala 
        115                 120                 125   

Ala Asn Gly Lys Val Tyr Gly Ala Pro Ala Val Ile Glu Ser Leu Val 
    130                 135                 140         

Met Tyr Tyr Asn Lys Asp Leu Val Lys Asp Ala Pro Lys Thr Phe Ala
145                 150                 155                 160

Asp Leu Glu Asn Leu Ala Lys Asp Ser Lys Tyr Ala Phe Ala Gly Glu 
                165                 170                 175

Asp Gly Lys Thr Thr Ala Phe Leu Ala Asp Trp Thr Asn Phe Tyr Tyr 
            180                 185                 190   

Thr Tyr Gly Leu Leu Ala Gly Asn Gly Ala Tyr Val Phe Gly Gln Asn 
        195                 200                 205       

Gly Lys Asp Ala Lys Asp Ile Gly Leu Ala Asn Asp Gly Ser Ile Val 
    210                 215                 220           

Gly Ile Asn Tyr Ala Lys Ser Trp Tyr Glu Lys Trp Pro Lys Gly Met
225                 230                 235                 240

Gln Asp Thr Glu Gly Ala Gly Asn Leu Ile Gln Thr Gln Phe Gln Glu 
                245                 250                 255

Gly Lys Thr Ala Ala Ile Ile Asp Gly Pro Trp Lys Ala Gln Ala Phe 
            260                 265                 270   

Lys Asp Ala Lys Val Asn Tyr Gly Val Ala Thr Ile Pro Thr Leu Pro 
        275                 280                 285      

Asn Gly Lys Glu Tyr Ala Ala Phe Gly Gly Gly Lys Ala Trp Val Ile 
    290                 295                 300         

Pro Gln Ala Val Lys Asn Leu Glu Ala Ser Gln Lys Phe Val Asp Phe
305                 310                 315                 320

Leu Val Ala Thr Glu Gln Gln Lys Val Leu Tyr Asp Lys Thr Asn Glu 
                325                 330                 335

Ile Pro Ala Asn Thr Glu Ala Arg Ser Tyr Ala Glu Gly Lys Asn Asp 
            340                 345                 350

Glu Leu Thr Thr Ala Val Ile Lys Gln Phe Lys Asn Thr Gln Pro Leu 
        355                 360                 365

Pro Asn Ile Ser Gln Met Ser Ala Val Trp Asp Pro Ala Lys Asn Met 
    370                 375                 380

Leu Phe Asp Ala Val Ser Gly Gln Lys Asp Ala Lys Thr Ala Ala Asn
385                 390                 395                 400

Asp Ala Val Thr Leu Ile Lys Glu Thr Leu Lys Gln Lys Phe Gly Glu
                405                 410                 415

<210> SEQ ID NO: 2
<211> LENGTH: 425
<212> TYPE: PRT
<213> ORGANISM: Yersinia enterocolitica

<220> FEATURE:
<223> OTHER INFORMATION: Unmodified TogB

<400> SEQUENCE: 2

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
  1               5                  10                  15   

Arg Gly Ser His Met Glu Val Asn Leu Arg Met Ser Trp Trp Gly Gly 
             20                  25                  30      

Asn Gly Arg His Gln Val Thr Leu Lys Ala Leu Glu Glu Phe His Lys 
         35                  40                  45      

Gln His Pro Asn Ile Asn Val Lys Ala Glu Tyr Thr Gly Trp Asp Gly 
     50                  55                  60         

His Leu Ser Arg Leu Thr Thr Gln Ile Ala Gly Gly Thr Glu Pro Asp
65                  70                  75                  80

Val Met Gln Thr Asn Trp Asn Trp Leu Pro Ile Phe Ser Lys Asp Gly 
                 85                  90                  95

Thr Gly Phe Tyr Asn Leu Phe Ser Val Lys Glu Gln Leu Asp Leu Ala 
            100                 105                 110   

Gln Phe Asp Pro Lys Glu Leu Gln Gln Thr Thr Val Asn Gly Lys Leu 
        115                 120                 125   

Asn Gly Ile Pro Ile Ser Val Thr Ala Arg Ile Phe Tyr Phe Asn Asp 
    130                 135                 140         

Ala Thr Trp Ala Lys Ala Gly Leu Glu Tyr Pro Lys Thr Trp Asp Glu
145                 150                 155                 160

Leu Leu Ala Ala Gly Lys Val Phe Lys Glu Lys Leu Gly Asp Gln Tyr
                165                 170                 175

Tyr Pro Val Val Leu Glu His Gln Asp Thr Leu Ala Leu Ile Arg Ser 
            180                 185                 190   

Tyr Met Thr Gln Lys Tyr Asn Ile Pro Thr Ile Asp Glu Ala Asn Lys 
        195                 200                 205       

Lys Phe Ala Tyr Ser Pro Glu Gln Trp Val Glu Phe Phe Thr Met Tyr 
    210                 215                 220           

Lys Thr Met Val Asp Asn His Val Met Pro Ser Thr Lys Tyr Tyr Ala
225                 230                 235                 240

Ser Phe Gly Lys Ser Asn Met Tyr Glu Met Lys Pro Trp Ile Asn Gly 
                245                 250                 255

Glu Trp Ala Gly Thr Tyr Met Trp Asn Ser Thr Ile Thr Lys Tyr Ser 
            260                 265                 270   

Asp Asn Leu Thr Lys Pro Ala Lys Leu Val Leu Gly Pro Tyr Pro Met 
        275                 280                 285      

Leu Pro Gly Ala Lys Asp Ala Gly Leu Phe Phe Lys Pro Ala Gln Met 
    290                 295                 300         

Leu Ser Ile Gly Lys Ser Thr Lys His Pro Gln Glu Ser Ala Met Leu
305                 310                 315                 320

Ile Asn Phe Leu Leu Asn Ser Lys Glu Gly Val Glu Ala Leu Gly Leu 
                325                 330                 335

Glu Arg Gly Val Pro Leu Ser Ala Thr Ala Val Thr Gln Leu Arg Ala 
            340                 345                 350

Ser Gly Val Ile Lys Asp Glu Asp Pro Ser Val Ala Gly Leu Asn Met 
        355                 360                 365

Ala Leu Glu Leu Pro His Lys Met Thr Thr Ser Pro Tyr Phe Asp Asp 
    370                 375                 380

Pro Gln Ile Val Ser Leu Phe Gly Asp Ala Ile Gln Tyr Ile Asp Tyr
385                 390                 395                 400

Gly Gln Lys Thr Val Gln Glu Thr Ala Glu Tyr Phe Asn Lys Gln Gly
                405                 410                 415
 
Asp Arg Ile Leu Lys Arg Ala Met Arg
            420                 425

<210> SEQ ID NO: 3
<211> LENGTH: 401
<212> TYPE: PRT
<213> ORGANISM: Escherichia coli

<220> FEATURE:
<223> OTHER INFORMATION: Unmodified EF-Tu

<400> SEQUENCE: 3

Met Ser Lys Glu Lys Phe Glu Arg Thr Lys Pro His Val Asn Val Gly
  1               5                  10                  15   

Thr Ile Gly His Val Asp His Gly Lys Thr Thr Leu Thr Ala Ala Ile
             20                  25                  30      

Thr Thr Val Leu Ala Lys Thr Tyr Gly Gly Ala Ala Arg Ala Phe Asp
         35                  40                  45      

Gln Ile Asp Asn Ala Pro Glu Glu Lys Ala Arg Gly Ile Thr Ile Asn
     50                  55                  60         

Thr Ser His Val Glu Tyr Asp Thr Pro Thr Arg His Tyr Ala His Val
 65                  70                  75                  80

Asp Cys Pro Gly His Ala Asp Tyr Val Lys Asn Met Ile Thr Gly Ala
                 85                  90                  95

Ala Gln Met Asp Gly Ala Ile Leu Val Val Ala Ala Thr Asp Gly Pro
            100                 105                 110   

Met Pro Gln Thr Arg Glu His Ile Leu Leu Gly Arg Gln Val Gly Val
        115                 120                 125   

Pro Tyr Ile Ile Val Phe Leu Asn Lys Cys Asp Met Val Asp Asp Glu
    130                 135                 140         

Glu Leu Leu Glu Leu Val Glu Met Glu Val Arg Glu Leu Leu Ser Gln
145                 150                 155                 160

Tyr Asp Phe Pro Gly Asp Asp Thr Pro Ile Val Arg Gly Ser Ala Leu
                165                 170                 175

Lys Ala Leu Glu Gly Asp Ala Glu Trp Glu Ala Lys Ile Leu Glu Leu
            180                 185                 190   

Ala Gly Phe Leu Asp Ser Tyr Ile Pro Glu Pro Glu Arg Ala Ile Asp
        195                 200                 205       

Lys Pro Phe Leu Leu Pro Ile Glu Asp Val Phe Ser Ile Ser Gly Arg
    210                 215                 220           

Gly Thr Val Val Thr Gly Arg Val Glu Arg Gly Ile Ile Lys Val Gly
225                 230                 235                 240

Glu Glu Val Glu Ile Val Gly Ile Lys Glu Thr Gln Lys Ser Thr Cys
                245                 250                 255

Thr Gly Val Glu Met Phe Arg Lys Leu Leu Asp Glu Gly Arg Ala Gly
            260                 265                 270   

Glu Asn Val Gly Val Leu Leu Arg Gly Ile Lys Arg Glu Glu Ile Glu
        275                 280                 285      

Arg Gly Gln Val Leu Ala Lys Pro Gly Thr Ile Lys Pro His Thr Lys
    290                 295                 300         

Phe Glu Ser Glu Val Tyr Ile Leu Ser Lys Asp Glu Gly Gly Arg His
305                 310                 315                 320

Thr Pro Phe Phe Lys Gly Tyr Arg Pro Gln Phe Tyr Phe Arg Thr Thr
                325                 330                 335

Asp Val Thr Gly Thr Ile Glu Leu Pro Glu Gly Val Glu Met Val Met
            340                 345                 350

Pro Gly Asp Asn Ile Lys Met Val Val Thr Leu Ile His Pro Ile Ala
        355                 360                 365

Met Asp Asp Gly Leu Arg Phe Ala Ile Arg Glu Gly Gly Arg Thr Val
    370                 375                 380

Gly Ala Gly Val Val Ala Lys Val Leu Gly Ser His His His His His
385                 390                 395                 400

His
