                         SEQUENCE LISTING

<110>  Snoretox Pty Ltd
 
<120>  COMPOSITION AND METHOD

<130>  P112158.PCT

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  1

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Ser 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Ser Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Ser  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Ser Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  2
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding TeNT

<400>  2
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga ttaccatcaa taattttcgt      240

tattcagatc cggtgaataa tgataccatc atcatgatgg aaccaccgta ctgtaaaggg      300

ctggatattt attataaagc gttcaaaatc accgaccgca tctggatcgt gccggaacgc      360

tacgaattcg gcaccaaacc ggaagatttt aatccgccga gtagtctgat cgaaggtgca      420

tcggaatact acgatccgaa ttatctgcgt actgactctg ataaagatcg ctttctgcaa      480

acgatggtaa aactgttcaa tcgtatcaaa aacaatgtag caggcgaagc cctgctggat      540

aaaatcatca acgccattcc gtatctggga aacagttatt ctctgctgga taaattcgat      600

acaaactcga actctgtgtc attcaacctg ctggaacagg acccgagcgg cgcgaccact      660

aagagtgcga tgctgactaa cctgattatt ttcggtccgg gaccggtact gaataaaaat      720

gaagttcgcg gcattgtact gcgtgtcgat aataaaaact atttcccatg tcgtgatggc      780

ttcggcagca tcatgcagat ggccttttgt ccggaatatg tgccaacttt cgataatgtg      840

attgagaaca tcacctctct gacgattggt aaaagtaaat atttccagga tccggctctg      900

ctgctgatgc atgaactgat ccatgttctg catggcctgt atggcatgca ggtttcatcc      960

cacgaaatta tcccatccaa acaggaaatt tacatgcagc atacatatcc gattagtgcc     1020

gaagaactgt tcacttttgg cggccaggat gcgaacctga tttcgattga cattaaaaac     1080

gatctgtatg aaaaaactct gaacgattat aaagcgattg ccaacaaact gtctcaggta     1140

acctcctgta acgatccgaa tattgatatt gacagttata aacaaattta tcagcagaag     1200

tatcagttcg ataaagactc taatggccag tatattgtta acgaagataa attccagatt     1260

ctgtacaata gcattatgta tggctttact gagatcgaac tgggtaaaaa atttaacatc     1320

aagactcgtc tgagctattt tagcatgaat catgatccag tgaaaatccc gaatctgctg     1380

gatgatacga tttataatga taccgaagga tttaacatcg aaagcaagga tctgaaatcc     1440

gaatataaag ggcagaacat gcgcgttaat accaatgcat ttcgcaatgt tgatggttca     1500

ggcctggtgt cgaaactgat tgggctgtgt aagaaaatca ttccaccgac aaatattcgc     1560

gaaaatctgt acaaccgtac ggcgagcctg accgatctgg ggggagaact gtgtattaaa     1620

atcaaaaatg aagatctgac cttcattgct gagaagaata gcttttccga agagccattc     1680

caggacgaaa tcgtgtctta taacaccaag aataaaccgc tgaatttcaa ctactccctg     1740

gacaaaatca ttgtggatta caacctgcag agtaaaatta ccctgccgaa tgatcgtacc     1800

accccggtga cgaaaggcat cccttacgca ccagaatata aatcaaatgc agcctcgact     1860

atcgagatcc ataatattga tgacaacact atttaccagt acctgtatgc tcagaaatct     1920

ccgacgacgc tgcagcgcat caccatgact aacagcgtgg acgatgccct gattaatagc     1980

accaaaatct actcttactt tccgtcggtg atctctaagg ttaatcaggg cgcgcaaggt     2040

atcctgtttc tgcaatgggt gcgtgatatt attgatgatt tcactaatga atctagccag     2100

aaaacgacaa ttgataaaat ttcggatgtt tccaccatcg tgccttacat cggcccagcg     2160

ctgaacatcg tgaagcaggg ttatgagggt aactttatcg gagcactgga aacgaccggc     2220

gtggttctgc tgctggaata tattccggag attactctgc cagttattgc ggctctgtcg     2280

attgcagaga gctcaacgca gaaagaaaaa attattaaga cgatcgacaa tttcctggaa     2340

aagcgctacg aaaaatggat cgaagtgtat aagctggtga aagcgaaatg gctggggacc     2400

gtgaacaccc agttccaaaa acgttcctat caaatgtatc gtagcctgga atatcaggtg     2460

gacgccatta aaaagatcat cgattacgaa tataagatct actccggtcc ggacaaagaa     2520

cagattgcgg acgaaattaa caatctgaaa aataaactgg aggaaaaagc caacaaagcg     2580

atgattaata tcaatatttt catgcgtgaa agcagccgta gcttcctggt caatcagatg     2640

attaatgaag cgaagaaaca actgctggaa tttgatacgc aatctaaaaa tattctgatg     2700

caatacatca aagccaattc taaatttatt gggatcacgg aactgaaaaa gctggaatcg     2760

aaaatcaata aagtctttag caccccgatt ccgttctcct actcgaaaaa tctggattgt     2820

tgggttgaca atgaagaaga tattgatgtt attctgaaaa agagcacgat cctgaacctg     2880

gatattaata acgatattat ctctgatatc agtggtttta attcatcagt tattacttac     2940

ccagacgctc aactggtgcc gggaatcaat gggaaagcca ttcatctggt gaataatgaa     3000

tcaagtgaag tgatcgtgca taaagcgatg gatatcgagt acaacgatat gtttaataat     3060

ttcacggtgt cgttctggct gcgtgttccg aaagtgagtg cctcccacct ggaacaatat     3120

ggaaccaacg aatactcaat cattagcagc atgaagaaac attcgctgag tattggttca     3180

ggttggagcg tttccctgaa agggaacaat ctgatctgga cactgaagga ctcagcgggc     3240

gaagtgcgtc agattacgtt tcgtgatctg ccggataaat ttaatgcata cctggctaac     3300

aaatgggtgt tcatcacaat caccaatgac cgtctgtcgt ctgcaaacct gtatattaat     3360

ggggtactga tgggctcggc agaaattaca gggctgggcg ccattcgtga agataacaat     3420

attacgctga aactggatcg ttgtaataac aataatcagt atgtgagcat tgataaattt     3480

cgtattttct gcaaagcgct gaacccgaaa gaaattgaaa aactgtatac ctcgtatctg     3540

tcaattacgt ttctgcgcga tttctgggga aacccgctgc gttacgatac ggaatactac     3600

ctgatcccgg tagccagttc tagtaaagac gttcaactga aaaatattac cgactacatg     3660

tatctgacaa acgctccatc atacacaaac ggcaaactga acatctatta ccgtcgcctg     3720

tacaatgggc tgaaattcat cattaaacgt tataccccga ataacgaaat tgattccttt     3780

gtgaagtccg gtgacttcat taagctgtat gtatcctata acaataatga acacatcgtt     3840

ggctatccga aggatggcaa tgcctttaac aacctggatc gtattctgcg tgtaggttac     3900

aacgccccgg gtattccgct gtataagaaa atggaagcag tgaaactgcg tgatctgaaa     3960

acatattccg tgcaactgaa gctgtatgat gacaaaaatg ctagcctggg tctggtaggc     4020

acgcataacg gtcagattgg aaacgatcct aatcgtgaca tcctgatcgc ctctaactgg     4080

tattttaacc acctgaaaga taaaattctg ggctgcgatt ggtattttgt ccctaccgat     4140

gaaggctgga cgaacgatta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


<210>  3
<211>  858
<212>  PRT
<213>  Clostridium tetani

<400>  3

Ser Leu Thr Asp Leu Gly Gly Glu Leu Cys Ile Lys Ile Lys Asn Glu 
1               5                   10                  15      


Asp Leu Thr Phe Ile Ala Glu Lys Asn Ser Phe Ser Glu Glu Pro Phe 
            20                  25                  30          


Gln Asp Glu Ile Val Ser Tyr Asn Thr Lys Asn Lys Pro Leu Asn Phe 
        35                  40                  45              


Asn Tyr Ser Leu Asp Lys Ile Ile Val Asp Tyr Asn Leu Gln Ser Lys 
    50                  55                  60                  


Ile Thr Leu Pro Asn Asp Arg Thr Thr Pro Val Thr Lys Gly Ile Pro 
65                  70                  75                  80  


Tyr Ala Pro Glu Tyr Lys Ser Asn Ala Ala Ser Thr Ile Glu Ile His 
                85                  90                  95      


Asn Ile Asp Asp Asn Thr Ile Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser 
            100                 105                 110         


Pro Thr Thr Leu Gln Arg Ile Thr Met Thr Asn Ser Val Asp Asp Ala 
        115                 120                 125             


Leu Ile Asn Ser Thr Lys Ile Tyr Ser Tyr Phe Pro Ser Val Ile Ser 
    130                 135                 140                 


Lys Val Asn Gln Gly Ala Gln Gly Ile Leu Phe Leu Gln Trp Val Arg 
145                 150                 155                 160 


Asp Ile Ile Asp Asp Phe Thr Asn Glu Ser Ser Gln Lys Thr Thr Ile 
                165                 170                 175     


Asp Lys Ile Ser Asp Val Ser Thr Ile Val Pro Tyr Ile Gly Pro Ala 
            180                 185                 190         


Leu Asn Ile Val Lys Gln Gly Tyr Glu Gly Asn Phe Ile Gly Ala Leu 
        195                 200                 205             


Glu Thr Thr Gly Val Val Leu Leu Leu Glu Tyr Ile Pro Glu Ile Thr 
    210                 215                 220                 


Leu Pro Val Ile Ala Ala Leu Ser Ile Ala Glu Ser Ser Thr Gln Lys 
225                 230                 235                 240 


Glu Lys Ile Ile Lys Thr Ile Asp Asn Phe Leu Glu Lys Arg Tyr Glu 
                245                 250                 255     


Lys Trp Ile Glu Val Tyr Lys Leu Val Lys Ala Lys Trp Leu Gly Thr 
            260                 265                 270         


Val Asn Thr Gln Phe Gln Lys Arg Ser Tyr Gln Met Tyr Arg Ser Leu 
        275                 280                 285             


Glu Tyr Gln Val Asp Ala Ile Lys Lys Ile Ile Asp Tyr Glu Tyr Lys 
    290                 295                 300                 


Ile Tyr Ser Gly Pro Asp Lys Glu Gln Ile Ala Asp Glu Ile Asn Asn 
305                 310                 315                 320 


Leu Lys Asn Lys Leu Glu Glu Lys Ala Asn Lys Ala Met Ile Asn Ile 
                325                 330                 335     


Asn Ile Phe Met Arg Glu Ser Ser Arg Ser Phe Leu Val Asn Gln Met 
            340                 345                 350         


Ile Asn Glu Ala Lys Lys Gln Leu Leu Glu Phe Asp Thr Gln Ser Lys 
        355                 360                 365             


Asn Ile Leu Met Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile 
    370                 375                 380                 


Thr Glu Leu Lys Lys Leu Glu Ser Lys Ile Asn Lys Val Phe Ser Thr 
385                 390                 395                 400 


Pro Ile Pro Phe Ser Tyr Ser Lys Asn Leu Asp Cys Trp Val Asp Asn 
                405                 410                 415     


Glu Glu Asp Ile Asp Val Ile Leu Lys Lys Ser Thr Ile Leu Asn Leu 
            420                 425                 430         


Asp Ile Asn Asn Asp Ile Ile Ser Asp Ile Ser Gly Phe Asn Ser Ser 
        435                 440                 445             


Val Ile Thr Tyr Pro Asp Ala Gln Leu Val Pro Gly Ile Asn Gly Lys 
    450                 455                 460                 


Ala Ile His Leu Val Asn Asn Glu Ser Ser Glu Val Ile Val His Lys 
465                 470                 475                 480 


Ala Met Asp Ile Glu Tyr Asn Asp Met Phe Asn Asn Phe Thr Val Ser 
                485                 490                 495     


Phe Trp Leu Arg Val Pro Lys Val Ser Ala Ser His Leu Glu Gln Tyr 
            500                 505                 510         


Gly Thr Asn Glu Tyr Ser Ile Ile Ser Ser Met Lys Lys His Ser Leu 
        515                 520                 525             


Ser Ile Gly Ser Gly Trp Ser Val Ser Leu Lys Gly Asn Asn Leu Ile 
    530                 535                 540                 


Trp Thr Leu Lys Asp Ser Ala Gly Glu Val Arg Gln Ile Thr Phe Arg 
545                 550                 555                 560 


Asp Leu Pro Asp Lys Phe Asn Ala Tyr Leu Ala Asn Lys Trp Val Phe 
                565                 570                 575     


Ile Thr Ile Thr Asn Asp Arg Leu Ser Ser Ala Asn Leu Tyr Ile Asn 
            580                 585                 590         


Gly Val Leu Met Gly Ser Ala Glu Ile Thr Gly Leu Gly Ala Ile Arg 
        595                 600                 605             


Glu Asp Asn Asn Ile Thr Leu Lys Leu Asp Arg Cys Asn Asn Asn Asn 
    610                 615                 620                 


Gln Tyr Val Ser Ile Asp Lys Phe Arg Ile Phe Cys Lys Ala Leu Asn 
625                 630                 635                 640 


Pro Lys Glu Ile Glu Lys Leu Tyr Thr Ser Tyr Leu Ser Ile Thr Phe 
                645                 650                 655     


Leu Arg Asp Phe Trp Gly Asn Pro Leu Arg Tyr Asp Thr Glu Tyr Tyr 
            660                 665                 670         


Leu Ile Pro Val Ala Ser Ser Ser Lys Asp Val Gln Leu Lys Asn Ile 
        675                 680                 685             


Thr Asp Tyr Met Tyr Leu Thr Asn Ala Pro Ser Tyr Thr Asn Gly Lys 
    690                 695                 700                 


Leu Asn Ile Tyr Tyr Arg Arg Leu Tyr Asn Gly Leu Lys Phe Ile Ile 
705                 710                 715                 720 


Lys Arg Tyr Thr Pro Asn Asn Glu Ile Asp Ser Phe Val Lys Ser Gly 
                725                 730                 735     


Asp Phe Ile Lys Leu Tyr Val Ser Tyr Asn Asn Asn Glu His Ile Val 
            740                 745                 750         


Gly Tyr Pro Lys Asp Gly Asn Ala Phe Asn Asn Leu Asp Arg Ile Leu 
        755                 760                 765             


Arg Val Gly Tyr Asn Ala Pro Gly Ile Pro Leu Tyr Lys Lys Met Glu 
    770                 775                 780                 


Ala Val Lys Leu Arg Asp Leu Lys Thr Tyr Ser Val Gln Leu Lys Leu 
785                 790                 795                 800 


Tyr Asp Asp Lys Asn Ala Ser Leu Gly Leu Val Gly Thr His Asn Gly 
                805                 810                 815     


Gln Ile Gly Asn Asp Pro Asn Arg Asp Ile Leu Ile Ala Ser Asn Trp 
            820                 825                 830         


Tyr Phe Asn His Leu Lys Asp Lys Ile Leu Gly Cys Asp Trp Tyr Phe 
        835                 840                 845             


Val Pro Thr Asp Glu Gly Trp Thr Asn Asp 
    850                 855             


<210>  4
<211>  451
<212>  PRT
<213>  Clostridium tetani

<400>  4

Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
1               5                   10                  15      


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
            20                  25                  30          


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
        35                  40                  45              


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
    50                  55                  60                  


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
65                  70                  75                  80  


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
                85                  90                  95      


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
            100                 105                 110         


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
        115                 120                 125             


Val Ser Leu Lys Gly Asn Asn Leu Ile Trp Thr Leu Lys Asp Ser Ala 
    130                 135                 140                 


Gly Glu Val Arg Gln Ile Thr Phe Arg Asp Leu Pro Asp Lys Phe Asn 
145                 150                 155                 160 


Ala Tyr Leu Ala Asn Lys Trp Val Phe Ile Thr Ile Thr Asn Asp Arg 
                165                 170                 175     


Leu Ser Ser Ala Asn Leu Tyr Ile Asn Gly Val Leu Met Gly Ser Ala 
            180                 185                 190         


Glu Ile Thr Gly Leu Gly Ala Ile Arg Glu Asp Asn Asn Ile Thr Leu 
        195                 200                 205             


Lys Leu Asp Arg Cys Asn Asn Asn Asn Gln Tyr Val Ser Ile Asp Lys 
    210                 215                 220                 


Phe Arg Ile Phe Cys Lys Ala Leu Asn Pro Lys Glu Ile Glu Lys Leu 
225                 230                 235                 240 


Tyr Thr Ser Tyr Leu Ser Ile Thr Phe Leu Arg Asp Phe Trp Gly Asn 
                245                 250                 255     


Pro Leu Arg Tyr Asp Thr Glu Tyr Tyr Leu Ile Pro Val Ala Ser Ser 
            260                 265                 270         


Ser Lys Asp Val Gln Leu Lys Asn Ile Thr Asp Tyr Met Tyr Leu Thr 
        275                 280                 285             


Asn Ala Pro Ser Tyr Thr Asn Gly Lys Leu Asn Ile Tyr Tyr Arg Arg 
    290                 295                 300                 


Leu Tyr Asn Gly Leu Lys Phe Ile Ile Lys Arg Tyr Thr Pro Asn Asn 
305                 310                 315                 320 


Glu Ile Asp Ser Phe Val Lys Ser Gly Asp Phe Ile Lys Leu Tyr Val 
                325                 330                 335     


Ser Tyr Asn Asn Asn Glu His Ile Val Gly Tyr Pro Lys Asp Gly Asn 
            340                 345                 350         


Ala Phe Asn Asn Leu Asp Arg Ile Leu Arg Val Gly Tyr Asn Ala Pro 
        355                 360                 365             


Gly Ile Pro Leu Tyr Lys Lys Met Glu Ala Val Lys Leu Arg Asp Leu 
    370                 375                 380                 


Lys Thr Tyr Ser Val Gln Leu Lys Leu Tyr Asp Asp Lys Asn Ala Ser 
385                 390                 395                 400 


Leu Gly Leu Val Gly Thr His Asn Gly Gln Ile Gly Asn Asp Pro Asn 
                405                 410                 415     


Arg Asp Ile Leu Ile Ala Ser Asn Trp Tyr Phe Asn His Leu Lys Asp 
            420                 425                 430         


Lys Ile Leu Gly Cys Asp Trp Tyr Phe Val Pro Thr Asp Glu Gly Trp 
        435                 440                 445             


Thr Asn Asp 
    450     


<210>  5
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  5

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Cys Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Cys Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Cys 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Cys His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Cys Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Cys Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Cys Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Cys His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Cys Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Cys  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Cys Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  6
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding the surface serine to cysteine 
       substituted mature TeNT of SEQ ID NO: 5

<400>  6
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga tcaccatcaa caacttccgt      240

tactctgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt      300

ctggacatct actacaaagc gttcaaaatc accgaccgta tctggatcgt tccggaacgt      360

tacgaattcg gtaccaaacc ggaagacttc aacccgccgt cttctctgat cgaaggtgcg      420

tctgaatact acgacccgaa ctacctgcgt accgactgcg acaaagaccg tttcctgcag      480

accatggtta aactgttcaa ccgtatcaaa aacaacgttg cgggtgaagc gctgctggac      540

aaaatcatca acgcgatccc gtacctgggt aactgctact ctctgctgga caaattcgac      600

accaactcta actctgtttc tttcaacctg ctggaacagg acccgtgcgg tgcgaccacc      660

aaatctgcga tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac      720

gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt      780

ttcggttcta tcatgcagat ggcgttctgc ccggaatacg ttccgacctt cgacaacgtt      840

atcgaaaaca tcacctctct gaccatcggt aaatctaaat acttccagga cccggcgctg      900

ctgctgatgc acgaactgat ccacgttctg cacggtctgt acggtatgca ggtttcttgc      960

cacgaaatca tcccgtctaa acaggaaatc tacatgcagc acacctaccc gatctctgcg     1020

gaagaactgt tcaccttcgg tggtcaggac gcgaacctga tctctatcga catcaaaaac     1080

gacctgtacg aaaaaaccct gaacgactac aaagcgatcg cgaacaaact gtctcaggtt     1140

acctcttgca acgacccgaa catcgacatc gactcttaca aacagatcta ccagcagaaa     1200

taccagttcg acaaagactg caacggtcag tacatcgtta acgaagacaa attccagatc     1260

ctgtacaact ctatcatgta cggtttcacc gaaatcgaac tgggtaaaaa attcaacatc     1320

aaaacccgtc tgtcttactt ctctatgaac cacgacccgg ttaaaatccc gaacctgctg     1380

gacgacacca tctacaacga caccgaaggt ttcaacatcg aatctaaaga cctgaaatct     1440

gaatacaaag gtcagaacat gcgtgttaac accaacgcgt tccgtaacgt tgacggttgc     1500

ggtctggttt ctaaactgat cggtctgtgc aaaaaaatca tcccgccgac caacatccgt     1560

gaaaacctgt acaaccgtac cgcgtctctg accgacctgg gtggtgaact gtgcatcaaa     1620

atcaaaaacg aagacctgac cttcatcgcg gaaaaaaact ctttctctga agaaccgttc     1680

caggacgaaa tcgtttctta caacaccaaa aacaaaccgc tgaacttcaa ctactctctg     1740

gacaaaatca tcgttgacta caacctgcag tctaaaatca ccctgccgaa cgaccgtacc     1800

accccggtta ccaaaggtat cccgtacgcg ccggaataca aatctaacgc ggcgtctacc     1860

atcgaaatcc acaacatcga cgacaacacc atctaccagt acctgtacgc gcagaaatct     1920

ccgaccaccc tgcagcgtat caccatgacc aactctgttg acgacgcgct gatcaactct     1980

accaaaatct actcttactt cccgtctgtt atctgcaaag ttaaccaggg tgcgcagggt     2040

atcctgttcc tgcagtgggt tcgtgacatc atcgacgact tcaccaacga atcttctcag     2100

aaaaccacca tcgacaaaat ctctgacgtt tctaccatcg ttccgtacat cggtccggcg     2160

ctgaacatcg ttaaacaggg ttacgaaggt aacttcatcg gtgcgctgga aaccaccggt     2220

gttgttctgc tgctggaata catcccggaa atcaccctgc cggttatcgc ggcgctgtct     2280

atcgcggaat cttctaccca gaaagaaaaa atcatcaaaa ccatcgacaa cttcctggaa     2340

aaacgttacg aaaaatggat cgaagtttac aaactggtta aagcgaaatg gctgggtacc     2400

gttaacaccc agttccagaa acgttcttac cagatgtacc gttctctgga ataccaggtt     2460

gacgcgatca aaaaaatcat cgactacgaa tacaaaatct actctggtcc ggacaaagaa     2520

cagatcgcgg acgaaatcaa caacctgaaa aacaaactgg aagaaaaagc gaacaaagcg     2580

atgatcaaca tcaacatctt catgcgtgaa tcttctcgtt ctttcctggt taaccagatg     2640

atcaacgaag cgaaaaaaca gctgctggaa ttcgacaccc agtctaaaaa catcctgatg     2700

cagtacatca aagcgaactc taaattcatc ggtatcaccg aactgaaaaa actggaatct     2760

aaaatcaaca aagttttctc taccccgatc ccgttctctt actctaaaaa cctggactgc     2820

tgggttgaca acgaagaaga catcgacgtt atcctgaaaa aatctaccat cctgaacctg     2880

gacatcaaca acgacatcat ctctgacatc tctggtttca actcttctgt tatcacctac     2940

ccggacgcgc agctggttcc gggtatcaac ggtaaagcga tccacctggt taacaacgaa     3000

tcttctgaag ttatcgttca caaagcgatg gacatcgaat acaacgacat gttcaacaac     3060

ttcaccgttt ctttctggct gcgtgttccg aaagtttctg cgtgccacct ggaacagtac     3120

ggtaccaacg aatactctat catctcttct atgaaaaaac actctctgtc tatcggttct     3180

ggttggtctg tttctctgaa aggtaacaac ctgatctgga ccctgaaaga ctctgcgggt     3240

gaagttcgtc agatcacctt ccgtgacctg ccggacaaat tcaacgcgta cctggcgaac     3300

aaatgggttt tcatcaccat caccaacgac cgtctgtgct ctgcgaacct gtacatcaac     3360

ggtgttctga tgggttctgc ggaaatcacc ggtctgggtg cgatccgtga agacaacaac     3420

atcaccctga aactggaccg ttgcaacaac aacaaccagt acgtttctat cgacaaattc     3480

cgtatcttct gcaaagcgct gaacccgaaa gaaatcgaaa aactgtacac ctcttacctg     3540

tctatcacct tcctgcgtga cttctggggt aacccgctgc gttacgacac cgaatactac     3600

ctgatcccgg ttgcgtcttc ttctaaagac gttcagctga aaaacatcac cgactacatg     3660

tacctgacca acgcgccgtg ctacaccaac ggtaaactga acatctacta ccgtcgtctg     3720

tacaacggtc tgaaattcat catcaaacgt tacaccccga acaacgaaat cgactgcttc     3780

gttaaatctg gtgacttcat caaactgtac gtttcttaca acaacaacga acacatcgtt     3840

ggttacccga aagacggtaa cgcgttcaac aacctggacc gtatcctgcg tgttggttac     3900

aacgcgccgg gtatcccgct gtacaaaaaa atggaagcgg ttaaactgcg tgacctgaaa     3960

acctactctg ttcagctgaa actgtacgac gacaaaaacg cgtctctggg tctggttggt     4020

acccacaacg gtcagatcgg taacgacccg aaccgtgaca tcctgatcgc gtctaactgg     4080

tacttcaacc acctgaaaga caaaatcctg ggttgcgact ggtacttcgt tccgaccgac     4140

gaaggttgga ccaacgacta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


<210>  7
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  7

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Cys Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Cys Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Cys 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Cys His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Cys Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Cys Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Cys His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Cys Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Cys  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Cys Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  8
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding the surface serine to cysteine 
       substituted mature TeNT of SEQ ID NO: 7

<400>  8
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga tcaccatcaa caacttccgt      240

tactctgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt      300

ctggacatct actacaaagc gttcaaaatc accgaccgta tctggatcgt tccggaacgt      360

tacgaattcg gtaccaaacc ggaagacttc aacccgccgt cttctctgat cgaaggtgcg      420

tctgaatact acgacccgaa ctacctgcgt accgactgcg acaaagaccg tttcctgcag      480

accatggtta aactgttcaa ccgtatcaaa aacaacgttg cgggtgaagc gctgctggac      540

aaaatcatca acgcgatccc gtacctgggt aactgctact ctctgctgga caaattcgac      600

accaactcta actctgtttc tttcaacctg ctggaacagg acccgtgcgg tgcgaccacc      660

aaatctgcga tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac      720

gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt      780

ttcggttcta tcatgcagat ggcgttctgc ccggaatacg ttccgacctt cgacaacgtt      840

atcgaaaaca tcacctctct gaccatcggt aaatctaaat acttccagga cccggcgctc      900

tgctgatgca cgaactgatc cacgttctgc acggtctgta cggtatgcag gtttcttgcc      960

acgaaatcat cccgtctaaa caggaaatct acatgcagca cacctacccg atctctgcgg     1020

aagaactgtt caccttcggt ggtcaggacg cgaacctgat ctctatcgac atcaaaaacg     1080

acctgtacga aaaaaccctg aacgactaca aagcgatcgc gaacaaactg tctcaggtta     1140

cctcttgcaa cgacccgaac atcgacatcg actcttacaa acagatctac cagcagaaat     1200

accagttcga caaagactgc aacggtcagt acatcgttaa cgaagacaaa ttccagatcc     1260

tgtacaactc tatcatgtac ggtttcaccg aaatcgaact gggtaaaaaa ttcaacatca     1320

aaacccgtct gtcttacttc tctatgaacc acgacccggt taaaatcccg aacctgctgg     1380

acgacaccat ctacaacgac accgaaggtt tcaacatcga atctaaagac ctgaaatctg     1440

aatacaaagg tcagaacatg cgtgttaaca ccaacgcgtt ccgtaacgtt gacggttgcg     1500

gtctggtttc taaactgatc ggtctgtgca aaaaaatcat cccgccgacc aacatccgtg     1560

aaaacctgta caaccgtacc gcgtctctga ccgacctggg tggtgaactg tgcatcaaaa     1620

tcaaaaacga agacctgacc ttcatcgcgg aaaaaaactc tttctctgaa gaaccgttcc     1680

aggacgaaat cgtttcttac aacaccaaaa acaaaccgct gaacttcaac tactctctgg     1740

acaaaatcat cgttgactac aacctgcagt ctaaaatcac cctgccgaac gaccgtacca     1800

ccccggttac caaaggtatc ccgtacgcgc cggaatacaa atctaacgcg gcgtctacca     1860

tcgaaatcca caacatcgac gacaacacca tctaccagta cctgtacgcg cagaaatctc     1920

cgaccaccct gcagcgtatc accatgacca actctgttga cgacgcgctg atcaactcta     1980

ccaaaatcta ctcttacttc ccgtctgtta tctctaaagt taaccagggt gcgcagggta     2040

tcctgttcct gcagtgggtt cgtgacatca tcgacgactt caccaacgaa tcttctcaga     2100

aaaccaccat cgacaaaatc tctgacgttt ctaccatcgt tccgtacatc ggtccggcgc     2160

tgaacatcgt taaacagggt tacgaaggta acttcatcgg tgcgctggaa accaccggtg     2220

ttgttctgct gctggaatac atcccggaaa tcaccctgcc ggttatcgcg gcgctgtcta     2280

tcgcggaatc ttctacccag aaagaaaaaa tcatcaaaac catcgacaac ttcctggaaa     2340

aacgttacga aaaatggatc gaagtttaca aactggttaa agcgaaatgg ctgggtaccg     2400

ttaacaccca gttccagaaa cgttcttacc agatgtaccg ttctctggaa taccaggttg     2460

acgcgatcaa aaaaatcatc gactacgaat acaaaatcta ctctggtccg gacaaagaac     2520

agatcgcgga cgaaatcaac aacctgaaaa acaaactgga agaaaaagcg aacaaagcga     2580

tgatcaacat caacatcttc atgcgtgaat cttctcgttc tttcctggtt aaccagatga     2640

tcaacgaagc gaaaaaacag ctgctggaat tcgacaccca gtctaaaaac atcctgatgc     2700

agtacatcaa agcgaactct aaattcatcg gtatcaccga actgaaaaaa ctggaatcta     2760

aaatcaacaa agttttctct accccgatcc cgttctctta ctctaaaaac ctggactgct     2820

gggttgacaa cgaagaagac atcgacgtta tcctgaaaaa atctaccatc ctgaacctgg     2880

acatcaacaa cgacatcatc tctgacatct ctggtttcaa ctcttctgtt atcacctacc     2940

cggacgcgca gctggttccg ggtatcaacg gtaaagcgat ccacctggtt aacaacgaat     3000

cttctgaagt tatcgttcac aaagcgatgg acatcgaata caacgacatg ttcaacaact     3060

tcaccgtttc tttctggctg cgtgttccga aagtttctgc gtgccacctg gaacagtacg     3120

gtaccaacga atactctatc atctcttcta tgaaaaaaca ctctctgtct atcggttctg     3180

gttggtctgt ttctctgaaa ggtaacaacc tgatctggac cctgaaagac tctgcgggtg     3240

aagttcgtca gatcaccttc cgtgacctgc cggacaaatt caacgcgtac ctggcgaaca     3300

aatgggtttt catcaccatc accaacgacc gtctgtgctc tgcgaacctg tacatcaacg     3360

gtgttctgat gggttctgcg gaaatcaccg gtctgggtgc gatccgtgaa gacaacaaca     3420

tcaccctgaa actggaccgt tgcaacaacc aacaaccagt acgtttctat cgacaaattc     3480

cgtatcttct gcaaagcgct gaacccgaaa gaaatcgaaa aactgtacac ctcttacctg     3540

tctatcacct tcctgcgtga cttctggggt aacccgctgc gttacgacac cgaatactac     3600

ctgatcccgg ttgcgtcttc ttctaaagac gttcagctga aaaacatcac cgactacatg     3660

tacctgacca acgcgccgtg ctacaccaac ggtaaactga acatctacta ccgtcgtctg     3720

tacaacggtc tgaaattcat catcaaacgt tacaccccga acaacgaaat cgactgcttc     3780

gttaaatctg gtgacttcat caaactgtac gtttcttaca acaacaacga acacatcgtt     3840

ggttacccga aagacggtaa cgcgttcaac aacctggacc gtatcctgcg tgttggttac     3900

aacgcgccgg gtatcccgct gtacaaaaaa atggaagcgg ttaaactgcg tgacctgaaa     3960

acctactctg ttcagctgaa actgtacgac gacaaaaacg cgtctctggg tctggttggt     4020

acccacaacg gtcagatcgg taacgacccg aaccgtgaca tcctgatcgc gtctaactgg     4080

tacttcaacc acctgaaaga caaaatcctg ggttgcgact ggtacttcgt tccgaccgac     4140

gaaggttgga ccaacgacta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


<210>  9
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  9

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Ser 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Cys Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Cys His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Cys Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Cys  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Cys Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  10
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding the surface serine to cysteine 
       substituted mature TeNT of SEQ ID NO: 9

<400>  10
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga tcaccatcaa caacttccgt      240

tactctgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt      300

ctggacatct actacaaagc gttcaaaatc accgaccgta tctggatcgt tccggaacgt      360

tacgaattcg gtaccaaacc ggaagacttc aacccgccgt cttctctgat cgaaggtgcg      420

tctgaatact acgacccgaa ctacctgcgt accgactctg acaaagaccg tttcctgcag      480

accatggtta aactgttcaa ccgtatcaaa aacaacgttg cgggtgaagc gctgctggac      540

aaaatcatca acgcgatccc gtacctgggt aactcttact ctctgctgga caaattcgac      600

accaactcta actctgtttc tttcaacctg ctggaacagg acccgtctgg tgcgaccacc      660

aaatctgcga tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac      720

gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt      780

ttcggttcta tcatgcagat ggcgttctgc ccggaatacg ttccgacctt cgacaacgtt      840

atcgaaaaca tcacctctct gaccatcggt aaatctaaat acttccagga cccggcgctg      900

ctgctgatgc acgaactgat ccacgttctg cacggtctgt acggtatgca ggtttcttct      960

cacgaaatca tcccgtctaa acaggaaatc tacatgcagc acacctaccc gatctctgcg     1020

gaagaactgt tcaccttcgg tggtcaggac gcgaacctga tctctatcga catcaaaaac     1080

gacctgtacg aaaaaaccct gaacgactac aaagcgatcg cgaacaaact gtctcaggtt     1140

acctcttgca acgacccgaa catcgacatc gactcttaca aacagatcta ccagcagaaa     1200

taccagttcg acaaagactc taacggtcag tacatcgtta acgaagacaa attccagatc     1260

ctgtacaact ctatcatgta cggtttcacc gaaatcgaac tgggtaaaaa attcaacatc     1320

aaaacccgtc tgtcttactt ctctatgaac cacgacccgg ttaaaatccc gaacctgctg     1380

gacgacacca tctacaacga caccgaaggt ttcaacatcg aatctaaaga cctgaaatct     1440

gaatacaaag gtcagaacat gcgtgttaac accaacgcgt tccgtaacgt tgacggttct     1500

ggtctggttt ctaaactgat cggtctgtgc aaaaaaatca tcccgccgac caacatccgt     1560

gaaaacctgt acaaccgtac cgcgtctctg accgacctgg gtggtgaact gtgcatcaaa     1620

atcaaaaacg aagacctgac cttcatcgcg gaaaaaaact ctttctctga agaaccgttc     1680

caggacgaaa tcgtttctta caacaccaaa aacaaaccgc tgaacttcaa ctactctctg     1740

gacaaaatca tcgttgacta caacctgcag tctaaaatca ccctgccgaa cgaccgtacc     1800

accccggtta ccaaaggtat cccgtacgcg ccggaataca aatctaacgc ggcgtctacc     1860

atcgaaatcc acaacatcga cgacaacacc atctaccagt acctgtacgc gcagaaatct     1920

ccgaccaccc tgcagcgtat caccatgacc aactctgttg acgacgcgct gatcaactct     1980

accaaaatct actcttactt cccgtctgtt atctgcaaag ttaaccaggg tgcgcagggt     2040

atcctgttcc tgcagtgggt tcgtgacatc atcgacgact tcaccaacga atcttctcag     2100

aaaaccacca tcgacaaaat ctctgacgtt tctaccatcg ttccgtacat cggtccggcg     2160

ctgaacatcg ttaaacaggg ttacgaaggt aacttcatcg gtgcgctgga aaccaccggt     2220

gttgttctgc tgctggaata catcccggaa atcaccctgc cggttatcgc ggcgctgtct     2280

atcgcggaat cttctaccca gaaagaaaaa atcatcaaaa ccatcgacaa cttcctggaa     2340

aaacgttacg aaaaatggat cgaagtttac aaactggtta aagcgaaatg gctgggtacc     2400

gttaacaccc agttccagaa acgttcttac cagatgtacc gttctctgga ataccaggtt     2460

gacgcgatca aaaaaatcat cgactacgaa tacaaaatct actctggtcc ggacaaagaa     2520

cagatcgcgg acgaaatcaa caacctgaaa aacaaactgg aagaaaaagc gaacaaagcg     2580

atgatcaaca tcaacatctt catgcgtgaa tcttctcgtt ctttcctggt taaccagatg     2640

atcaacgaag cgaaaaaaca gctgctggaa ttcgacaccc agtctaaaaa catcctgatg     2700

cagtacatca aagcgaactc taaattcatc ggtatcaccg aactgaaaaa actggaatct     2760

aaaatcaaca aagttttctc taccccgatc ccgttctctt actctaaaaa cctggactgc     2820

tgggttgaca acgaagaaga catcgacgtt atcctgaaaa aatctaccat cctgaacctg     2880

gacatcaaca acgacatcat ctctgacatc tctggtttca actcttctgt tatcacctac     2940

ccggacgcgc agctggttcc gggtatcaac ggtaaagcga tccacctggt taacaacgaa     3000

tcttctgaag ttatcgttca caaagcgatg gacatcgaat acaacgacat gttcaacaac     3060

ttcaccgttt ctttctggct gcgtgttccg aaagtttctg cgtgccacct ggaacagtac     3120

ggtaccaacg aatactctat catctcttct atgaaaaaac actctctgtc tatcggttct     3180

ggttggtctg tttctctgaa aggtaacaac ctgatctgga ccctgaaaga ctctgcgggt     3240

gaagttcgtc agatcacctt ccgtgacctg ccggacaaat tcaacgcgta cctggcgaac     3300

aaatgggttt tcatcaccat caccaacgac cgtctgtgct ctgcgaacct gtacatcaac     3360

ggtgttctga tgggttctgc ggaaatcacc ggtctgggtg cgatccgtga agacaacaac     3420

atcaccctga aactggaccg ttgcaacaac aacaaccagt acgtttctat cgacaaattc     3480

cgtatcttct gcaaagcgct gaacccgaaa gaaatcgaaa aactgtacac ctcttacctg     3540

tctatcacct tcctgcgtga cttctggggt aacccgctgc gttacgacac cgaatactac     3600

ctgatcccgg ttgcgtcttc ttctaaagac gttcagctga aaaacatcac cgactacatg     3660

tacctgacca acgcgccgtg ctacaccaac ggtaaactga acatctacta ccgtcgtctg     3720

tacaacggtc tgaaattcat catcaaacgt tacaccccga acaacgaaat cgactgcttc     3780

gttaaatctg gtgacttcat caaactgtac gtttcttaca acaacaacga acacatcgtt     3840

ggttacccga aagacggtaa cgcgttcaac aacctggacc gtatcctgcg tgttggttac     3900

aacgcgccgg gtatcccgct gtacaaaaaa atggaagcgg ttaaactgcg tgacctgaaa     3960

acctactctg ttcagctgaa actgtacgac gacaaaaacg cgtctctggg tctggttggt     4020

acccacaacg gtcagatcgg taacgacccg aaccgtgaca tcctgatcgc gtctaactgg     4080

tacttcaacc acctgaaaga caaaatcctg ggttgcgact ggtacttcgt tccgaccgac     4140

gaaggttgga ccaacgacta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


<210>  11
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  11

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Cys Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Cys Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Cys 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Cys His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Cys Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Cys Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Cys His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Cys Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Cys  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Cys Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  12
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding the surface serine to cysteine 
       substituted mature TeNT of SEQ ID NO: 11

<400>  12
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga tcaccatcaa caacttccgt      240

tactctgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt      300

ctggacatct actacaaagc gttcaaaatc accgaccgta tctggatcgt tccggaacgt      360

tacgaattcg gtaccaaacc ggaagacttc aacccgccgt cttctctgat cgaaggtgcg      420

tctgaatact acgacccgaa ctacctgcgt accgactgcg acaaagaccg tttcctgcag      480

accatggtta aactgttcaa ccgtatcaaa aacaacgttg cgggtgaagc gctgctggac      540

aaaatcatca acgcgatccc gtacctgggt aactgctact ctctgctgga caaattcgac      600

accaactcta actctgtttc tttcaacctg ctggaacagg acccgtgcgg tgcgaccacc      660

aaatctgcga tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac      720

gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt      780

ttcggttcta tcatgcagat ggcgttctgc ccggaatacg ttccgacctt cgacaacgtt      840

atcgaaaaca tcacctctct gaccatcggt aaatctaaat acttccagga cccggcgctg      900

ctgctgatgc acgaactgat ccacgttctg cacggtctgt acggtatgca ggtttcttgc      960

cacgaaatca tcccgtctaa acaggaaatc tacatgcagc acacctaccc gatctctgcg     1020

gaagaactgt tcaccttcgg tggtcaggac gcgaacctga tctctatcga catcaaaaac     1080

gacctgtacg aaaaaaccct gaacgactac aaagcgatcg cgaacaaact gtctcaggtt     1140

acctcttgca acgacccgaa catcgacatc gactcttaca aacagatcta ccagcagaaa     1200

taccagttcg acaaagactg caacggtcag tacatcgtta acgaagacaa attccagatc     1260

ctgtacaact ctatcatgta cggtttcacc gaaatcgaac tgggtaaaaa attcaacatc     1320

aaaacccgtc tgtcttactt ctctatgaac cacgacccgg ttaaaatccc gaacctgctg     1380

gacgacacca tctacaacga caccgaaggt ttcaacatcg aatctaaaga cctgaaatct     1440

gaatacaaag gtcagaacat gcgtgttaac accaacgcgt tccgtaacgt tgacggttgc     1500

ggtctggttt ctaaactgat cggtctgtgc aaaaaaatca tcccgccgac caacatccgt     1560

gaaaacctgt acaaccgtac cgcgtctctg accgacctgg gtggtgaact gtgcatcaaa     1620

atcaaaaacg aagacctgac cttcatcgcg gaaaaaaact ctttctctga agaaccgttc     1680

caggacgaaa tcgtttctta caacaccaaa aacaaaccgc tgaacttcaa ctactctctg     1740

gacaaaatca tcgttgacta caacctgcag tctaaaatca ccctgccgaa cgaccgtacc     1800

accccggtta ccaaaggtat cccgtacgcg ccggaataca aatctaacgc ggcgtctacc     1860

atcgaaatcc acaacatcga cgacaacacc atctaccagt acctgtacgc gcagaaatct     1920

ccgaccaccc tgcagcgtat caccatgacc aactctgttg acgacgcgct gatcaactct     1980

accaaaatct actcttactt cccgtctgtt atctctaaag ttaaccaggg tgcgcagggt     2040

atcctgttcc tgcagtgggt tcgtgacatc atcgacgact tcaccaacga atcttctcag     2100

aaaaccacca tcgacaaaat ctctgacgtt tctaccatcg ttccgtacat cggtccggcg     2160

ctgaacatcg ttaaacaggg ttacgaaggt aacttcatcg gtgcgctgga aaccaccggt     2220

gttgttctgc tgctggaata catcccggaa atcaccctgc cggttatcgc ggcgctgtct     2280

atcgcggaat cttctaccca gaaagaaaaa atcatcaaaa ccatcgacaa cttcctggaa     2340

aaacgttacg aaaaatggat cgaagtttac aaactggtta aagcgaaatg gctgggtacc     2400

gttaacaccc agttccagaa acgttcttac cagatgtacc gttctctgga ataccaggtt     2460

gacgcgatca aaaaaatcat cgactacgaa tacaaaatct actctggtcc ggacaaagaa     2520

cagatcgcgg acgaaatcaa caacctgaaa aacaaactgg aagaaaaagc gaacaaagcg     2580

atgatcaaca tcaacatctt catgcgtgaa tcttctcgtt ctttcctggt taaccagatg     2640

atcaacgaag cgaaaaaaca gctgctggaa ttcgacaccc agtctaaaaa catcctgatg     2700

cagtacatca aagcgaactc taaattcatc ggtatcaccg aactgaaaaa actggaatct     2760

aaaatcaaca aagttttctc taccccgatc ccgttctctt actctaaaaa cctggactgc     2820

tgggttgaca acgaagaaga catcgacgtt atcctgaaaa aatctaccat cctgaacctg     2880

gacatcaaca acgacatcat ctctgacatc tctggtttca actcttctgt tatcacctac     2940

ccggacgcgc agctggttcc gggtatcaac ggtaaagcga tccacctggt taacaacgaa     3000

tcttctgaag ttatcgttca caaagcgatg gacatcgaat acaacgacat gttcaacaac     3060

ttcaccgttt ctttctggct gcgtgttccg aaagtttctg cgtgccacct ggaacagtac     3120

ggtaccaacg aatactctat catctcttct atgaaaaaac actctctgtc tatcggttct     3180

ggttggtctg tttctctgaa aggtaacaac ctgatctgga ccctgaaaga ctctgcgggt     3240

gaagttcgtc agatcacctt ccgtgacctg ccggacaaat tcaacgcgta cctggcgaac     3300

aaatgggttt tcatcaccat caccaacgac cgtctgtgct ctgcgaacct gtacatcaac     3360

ggtgttctga tgggttctgc ggaaatcacc ggtctgggtg cgatccgtga agacaacaac     3420

atcaccctga aactggaccg ttgcaacaac aacaaccagt acgtttctat cgacaaattc     3480

cgtatcttct gcaaagcgct gaacccgaaa gaaatcgaaa aactgtacac ctcttacctg     3540

tctatcacct tcctgcgtga cttctggggt aacccgctgc gttacgacac cgaatactac     3600

ctgatcccgg ttgcgtcttc ttctaaagac gttcagctga aaaacatcac cgactacatg     3660

tacctgacca acgcgccgtg ctacaccaac ggtaaactga acatctacta ccgtcgtctg     3720

tacaacggtc tgaaattcat catcaaacgt tacaccccga acaacgaaat cgactgcttc     3780

gttaaatctg gtgacttcat caaactgtac gtttcttaca acaacaacga acacatcgtt     3840

ggttacccga aagacggtaa cgcgttcaac aacctggacc gtatcctgcg tgttggttac     3900

aacgcgccgg gtatcccgct gtacaaaaaa atggaagcgg ttaaactgcg tgacctgaaa     3960

acctactctg ttcagctgaa actgtacgac gacaaaaacg cgtctctggg tctggttggt     4020

acccacaacg gtcagatcgg taacgacccg aaccgtgaca tcctgatcgc gtctaactgg     4080

tacttcaacc acctgaaaga caaaatcctg ggttgcgact ggtacttcgt tccgaccgac     4140

gaaggttgga ccaacgacta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


<210>  13
<211>  1314
<212>  PRT
<213>  Clostridium tetani

<400>  13

Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn Asp 
1               5                   10                  15      


Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile Tyr 
            20                  25                  30          


Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu Arg 
        35                  40                  45              


Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser Leu 
    50                  55                  60                  


Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn Arg 
                85                  90                  95      


Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile Asn 
            100                 105                 110         


Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe Asp 
        115                 120                 125             


Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro Ser 
    130                 135                 140                 


Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe Gly 
145                 150                 155                 160 


Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu Arg 
                165                 170                 175     


Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser Ile 
            180                 185                 190         


Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn Val 
        195                 200                 205             


Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe Gln 
    210                 215                 220                 


Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His Gly 
225                 230                 235                 240 


Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys Gln 
                245                 250                 255     


Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu Phe 
            260                 265                 270         


Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys Asn 
        275                 280                 285             


Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn Lys 
    290                 295                 300                 


Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser Asn 
                325                 330                 335     


Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn Ser 
            340                 345                 350         


Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn Ile 
        355                 360                 365             


Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys Ile 
    370                 375                 380                 


Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe Asn 
385                 390                 395                 400 


Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met Arg 
                405                 410                 415     


Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val Ser 
            420                 425                 430         


Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg 
        435                 440                 445             


Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu 
    450                 455                 460                 


Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu Lys 
465                 470                 475                 480 


Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr Asn 
                485                 490                 495     


Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile Ile 
            500                 505                 510         


Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg Thr 
        515                 520                 525             


Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser Asn 
    530                 535                 540                 


Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile Tyr 
545                 550                 555                 560 


Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile Thr 
                565                 570                 575     


Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile Tyr 
            580                 585                 590         


Ser Tyr Phe Pro Ser Val Ile Cys Lys Val Asn Gln Gly Ala Gln Gly 
        595                 600                 605             


Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr Asn 
    610                 615                 620                 


Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser Thr 
625                 630                 635                 640 


Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly Tyr 
                645                 650                 655     


Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu Leu 
            660                 665                 670         


Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu Ser 
        675                 680                 685             


Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile Asp 
    690                 695                 700                 


Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys Leu 
705                 710                 715                 720 


Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys Arg 
                725                 730                 735     


Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile Lys 
            740                 745                 750         


Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys Glu 
        755                 760                 765             


Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu Lys 
    770                 775                 780                 


Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser Ser 
785                 790                 795                 800 


Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln Leu 
                805                 810                 815     


Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile Lys 
            820                 825                 830         


Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu Ser 
        835                 840                 845             


Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser Lys 
    850                 855                 860                 


Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile Leu 
865                 870                 875                 880 


Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile Ser 
                885                 890                 895     


Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala Gln 
            900                 905                 910         


Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn Glu 
        915                 920                 925             


Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn Asp 
    930                 935                 940                 


Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val 
945                 950                 955                 960 


Ser Ala Cys His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile Ile 
                965                 970                 975     


Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser Val 
            980                 985                 990         


Ser Leu Lys Gly Asn Asn Leu Ile  Trp Thr Leu Lys Asp  Ser Ala Gly 
        995                 1000                 1005             


Glu Val  Arg Gln Ile Thr Phe  Arg Asp Leu Pro Asp  Lys Phe Asn 
    1010                 1015                 1020             


Ala Tyr  Leu Ala Asn Lys Trp  Val Phe Ile Thr Ile  Thr Asn Asp 
    1025                 1030                 1035             


Arg Leu  Cys Ser Ala Asn Leu  Tyr Ile Asn Gly Val  Leu Met Gly 
    1040                 1045                 1050             


Ser Ala  Glu Ile Thr Gly Leu  Gly Ala Ile Arg Glu  Asp Asn Asn 
    1055                 1060                 1065             


Ile Thr  Leu Lys Leu Asp Arg  Cys Asn Asn Asn Asn  Gln Tyr Val 
    1070                 1075                 1080             


Ser Ile  Asp Lys Phe Arg Ile  Phe Cys Lys Ala Leu  Asn Pro Lys 
    1085                 1090                 1095             


Glu Ile  Glu Lys Leu Tyr Thr  Ser Tyr Leu Ser Ile  Thr Phe Leu 
    1100                 1105                 1110             


Arg Asp  Phe Trp Gly Asn Pro  Leu Arg Tyr Asp Thr  Glu Tyr Tyr 
    1115                 1120                 1125             


Leu Ile  Pro Val Ala Ser Ser  Ser Lys Asp Val Gln  Leu Lys Asn 
    1130                 1135                 1140             


Ile Thr  Asp Tyr Met Tyr Leu  Thr Asn Ala Pro Cys  Tyr Thr Asn 
    1145                 1150                 1155             


Gly Lys  Leu Asn Ile Tyr Tyr  Arg Arg Leu Tyr Asn  Gly Leu Lys 
    1160                 1165                 1170             


Phe Ile  Ile Lys Arg Tyr Thr  Pro Asn Asn Glu Ile  Asp Cys Phe 
    1175                 1180                 1185             


Val Lys  Ser Gly Asp Phe Ile  Lys Leu Tyr Val Ser  Tyr Asn Asn 
    1190                 1195                 1200             


Asn Glu  His Ile Val Gly Tyr  Pro Lys Asp Gly Asn  Ala Phe Asn 
    1205                 1210                 1215             


Asn Leu  Asp Arg Ile Leu Arg  Val Gly Tyr Asn Ala  Pro Gly Ile 
    1220                 1225                 1230             


Pro Leu  Tyr Lys Lys Met Glu  Ala Val Lys Leu Arg  Asp Leu Lys 
    1235                 1240                 1245             


Thr Tyr  Ser Val Gln Leu Lys  Leu Tyr Asp Asp Lys  Asn Ala Ser 
    1250                 1255                 1260             


Leu Gly  Leu Val Gly Thr His  Asn Gly Gln Ile Gly  Asn Asp Pro 
    1265                 1270                 1275             


Asn Arg  Asp Ile Leu Ile Ala  Ser Asn Trp Tyr Phe  Asn His Leu 
    1280                 1285                 1290             


Lys Asp  Lys Ile Leu Gly Cys  Asp Trp Tyr Phe Val  Pro Thr Asp 
    1295                 1300                 1305             


Glu Gly  Trp Thr Asn Asp 
    1310                 


<210>  14
<211>  6816
<212>  DNA
<213>  Artificial sequence

<220>
<223>  vector pRSET-TeNT encoding the surface serine to cysteine 
       substituted mature TeNT of SEQ ID NO: 13

<400>  14
gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat      120

catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac      180

gatgacgata aggatcgatg gggatccgag ctcgagccga tcaccatcaa caacttccgt      240

tactctgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt      300

ctggacatct actacaaagc gttcaaaatc accgaccgta tctggatcgt tccggaacgt      360

tacgaattcg gtaccaaacc ggaagacttc aacccgccgt cttctctgat cgaaggtgcg      420

tctgaatact acgacccgaa ctacctgcgt accgactctg acaaagaccg tttcctgcag      480

accatggtta aactgttcaa ccgtatcaaa aacaacgttg cgggtgaagc gctgctggac      540

aaaatcatca acgcgatccc gtacctgggt aactcttact ctctgctgga caaattcgac      600

accaactcta actctgtttc tttcaacctg ctggaacagg acccgtctgg tgcgaccacc      660

aaatctgcga tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac      720

gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt      780

ttcggttcta tcatgcagat ggcgttctgc ccggaatacg ttccgacctt cgacaacgtt      840

atcgaaaaca tcacctctct gaccatcggt aaatctaaat acttccagga cccggcgctg      900

ctgctgatgc acgaactgat ccacgttctg cacggtctgt acggtatgca ggtttcttct      960

cacgaaatca tcccgtctaa acaggaaatc tacatgcagc acacctaccc gatctctgcg     1020

gaagaactgt tcaccttcgg tggtcaggac gcgaacctga tctctatcga catcaaaaac     1080

gacctgtacg aaaaaaccct gaacgactac aaagcgatcg cgaacaaact gtctcaggtt     1140

acctcttgca acgacccgaa catcgacatc gactcttaca aacagatcta ccagcagaaa     1200

taccagttcg acaaagactc taacggtcag tacatcgtta acgaagacaa attccagatc     1260

ctgtacaact ctatcatgta cggtttcacc gaaatcgaac tgggtaaaaa attcaacatc     1320

aaaacccgtc tgtcttactt ctctatgaac cacgacccgg ttaaaatccc gaacctgctg     1380

gacgacacca tctacaacga caccgaaggt ttcaacatcg aatctaaaga cctgaaatct     1440

gaatacaaag gtcagaacat gcgtgttaac accaacgcgt tccgtaacgt tgacggttct     1500

ggtctggttt ctaaactgat cggtctgtgc aaaaaaatca tcccgccgac caacatccgt     1560

gaaaacctgt acaaccgtac cgcgtctctg accgacctgg gtggtgaact gtgcatcaaa     1620

atcaaaaacg aagacctgac cttcatcgcg gaaaaaaact ctttctctga agaaccgttc     1680

caggacgaaa tcgtttctta caacaccaaa aacaaaccgc tgaacttcaa ctactctctg     1740

gacaaaatca tcgttgacta caacctgcag tctaaaatca ccctgccgaa cgaccgtacc     1800

accccggtta ccaaaggtat cccgtacgcg ccggaataca aatctaacgc ggcgtctacc     1860

atcgaaatcc acaacatcga cgacaacacc atctaccagt acctgtacgc gcagaaatct     1920

ccgaccaccc tgcagcgtat caccatgacc aactctgttg acgacgcgct gatcaactct     1980

accaaaatct actcttactt cccgtctgtt atctgcaaag ttaaccaggg tgcgcagggt     2040

atcctgttcc tgcagtgggt tcgtgacatc atcgacgact tcaccaacga atcttctcag     2100

aaaaccacca tcgacaaaat ctctgacgtt tctaccatcg ttccgtacat cggtccggcg     2160

ctgaacatcg ttaaacaggg ttacgaaggt aacttcatcg gtgcgctgga aaccaccggt     2220

gttgttctgc tgctggaata catcccggaa atcaccctgc cggttatcgc ggcgctgtct     2280

atcgcggaat cttctaccca gaaagaaaaa atcatcaaaa ccatcgacaa cttcctggaa     2340

aaacgttacg aaaaatggat cgaagtttac aaactggtta aagcgaaatg gctgggtacc     2400

gttaacaccc agttccagaa acgttcttac cagatgtacc gttctctgga ataccaggtt     2460

gacgcgatca aaaaaatcat cgactacgaa tacaaaatct actctggtcc ggacaaagaa     2520

cagatcgcgg acgaaatcaa caacctgaaa aacaaactgg aagaaaaagc gaacaaagcg     2580

atgatcaaca tcaacatctt catgcgtgaa tcttctcgtt ctttcctggt taaccagatg     2640

atcaacgaag cgaaaaaaca gctgctggaa ttcgacaccc agtctaaaaa catcctgatg     2700

cagtacatca aagcgaactc taaattcatc ggtatcaccg aactgaaaaa actggaatct     2760

aaaatcaaca aagttttctc taccccgatc ccgttctctt actctaaaaa cctggactgc     2820

tgggttgaca acgaagaaga catcgacgtt atcctgaaaa aatctaccat cctgaacctg     2880

gacatcaaca acgacatcat ctctgacatc tctggtttca actcttctgt tatcacctac     2940

ccggacgcgc agctggttcc gggtatcaac ggtaaagcga tccacctggt taacaacgaa     3000

tcttctgaag ttatcgttca caaagcgatg gacatcgaat acaacgacat gttcaacaac     3060

ttcaccgttt ctttctggct gcgtgttccg aaagtttctg cgtgccacct ggaacagtac     3120

ggtaccaacg aatactctat catctcttct atgaaaaaac actctctgtc tatcggttct     3180

ggttggtctg tttctctgaa aggtaacaac ctgatctgga ccctgaaaga ctctgcgggt     3240

gaagttcgtc agatcacctt ccgtgacctg ccggacaaat tcaacgcgta cctggcgaac     3300

aaatgggttt tcatcaccat caccaacgac cgtctgtgct ctgcgaacct gtacatcaac     3360

ggtgttctga tgggttctgc ggaaatcacc ggtctgggtg cgatccgtga agacaacaac     3420

atcaccctga aactggaccg ttgcaacaac aacaaccagt acgtttctat cgacaaattc     3480

cgtatcttct gcaaagcgct gaacccgaaa gaaatcgaaa aactgtacac ctcttacctg     3540

tctatcacct tcctgcgtga cttctggggt aacccgctgc gttacgacac cgaatactac     3600

ctgatcccgg ttgcgtcttc ttctaaagac gttcagctga aaaacatcac cgactacatg     3660

tacctgacca acgcgccgtg ctacaccaac ggtaaactga acatctacta ccgtcgtctg     3720

tacaacggtc tgaaattcat catcaaacgt tacaccccga acaacgaaat cgactgcttc     3780

gttaaatctg gtgacttcat caaactgtac gtttcttaca acaacaacga acacatcgtt     3840

ggttacccga aagacggtaa cgcgttcaac aacctggacc gtatcctgcg tgttggttac     3900

aacgcgccgg gtatcccgct gtacaaaaaa atggaagcgg ttaaactgcg tgacctgaaa     3960

acctactctg ttcagctgaa actgtacgac gacaaaaacg cgtctctggg tctggttggt     4020

acccacaacg gtcagatcgg taacgacccg aaccgtgaca tcctgatcgc gtctaactgg     4080

tacttcaacc acctgaaaga caaaatcctg ggttgcgact ggtacttcgt tccgaccgac     4140

gaaggttgga ccaacgacta aaagcttgat ccggctgcta acaaagcccg aaaggaagct     4200

gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     4260

gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt aatagcgaag     4320

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4380

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4440

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4500

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4560

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4620

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4680

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4740

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4800

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4860

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4920

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4980

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     5040

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     5100

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     5160

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     5220

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     5280

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5340

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5400

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5460

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5520

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5580

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5640

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5700

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5760

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5820

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5880

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5940

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     6000

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     6060

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     6120

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     6180

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     6240

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     6300

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6360

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6420

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6480

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6540

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6600

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6660

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6720

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6780

gcctctcccc gcgcgttggc cgattcatta atgcag                               6816


