                         SEQUENCE LISTING

<110>  General Probiotics, Inc.
 
<120>  COMPOSITIONS INCLUDING PROBIOTIC BACTERIA FOR THE EXPRESSION AND 
       SECRETION OF ENTEROCINS TO CONTROL CLOSTRIDIA PERFRINGENS-INDUCED
        NECROTIC ENTERITIS IN LIVESTOCK AND RELATED METHODS

<130>  PDSD 269.0002WOU1

<150>  US 62/966,354
<151>  2020-01-27

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  424
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of construct to express Enterocin A

<400>  1
ttgacggcta gctcagtcct aggtacagtg ctagctactt acataacaac agcaacaact       60

aaggaggttt tcaatgcgca ctctgactct gaatgaatta gattctgttt ctggtggtac      120

cactcatagc ggtaagtatt acggaaatgg agtttactgt accaaaaata aatgcaccgt      180

tgattgggct aaagcgacaa cttgtatcgc tggtatgtct atcggcgggt tcttaggggg      240

tgccattcca ggcaaatgct aagcttggtc gaagctcaga ggatcgtaca ggcttccagg      300

catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg      360

tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt      420

tata                                                                   424


<210>  2
<211>  3632
<212>  DNA
<213>  Escherichia coli

<400>  2
cctgataact ctcctatgtt gtatgtttat atgattttcc ttgaaacata taatgcaaat       60

tttcgattta ttttccatca ttaatccaga taaacaacaa actaatagta tgcaaggaga      120

cattatttgt ttcgccatga tgctttagaa aacagaaaaa tgaagtggca gggacgggca      180

atattacttc ccggaatacc actgtggtta atcatgctgg gaagcattgt gtttattacg      240

gcatttctga tgttcattat tgttggtacc tatagccgcc gtgttaatgt cagtggtgag      300

gtcacaacct ggccaagagc tgtcaatata tattcaggtg tacagggatt tgttgtcagg      360

cagtttgttc atgaagggca gttgataaaa aaaggggatc ctgtttatct gattgacatc      420

agtaaaagta cacgcaatgg tattgtcact gataatcatc gccgggatat agaaaaccag      480

ctggttcgtg tggacaacat tatttcccgt ctggaagaaa gtaaaaaaat aacgctagat      540

accctggaaa aacaacgtct gcaatacaca gatgcgttcc gtcgctcatc agacattata      600

cagcgtgcag aggaagggat aaaaataatg aaaaataata tggagaatta cagatactat      660

cagtcaaaag gactgattaa taaagatcaa ttaactaacc aagttgcatt atattatcaa      720

caacaaaaca accttctcag tctgagcgga caaaatgaac aaaatgccct gcagataacc      780

actctggaga gtcagattca gactcaggca gcagattttg ataatcgtat ctatcagatg      840

gaactgcaac gactcgaatt gcagaaagaa ctggttaaca ctgatgtgga aggcgaaatc      900

attatccggg cgttgtctga cgggaaagtt gactccctga gtgtcactgt agggcaaatg      960

gtcaataccg gagacagcct tctgcaggtt attcctgaga acattgaaaa ctattatctt     1020

attctctggg tcccgaatga tgctgttcct tatatttcgg ctggtgacaa agtgaatatt     1080

cgttatgaag ccttcccctc agaaaaattt gggcagttct ctgctacggt taaaactata     1140

tccaggactc ctgcgtcaac acaggaaatg ttgacctata agggagcacc tcaaaatacg     1200

ccgggtgcct ctgttccctg gtataaagtc attgcgacgc ctgaaaagca gataatcagg     1260

tatgacgaaa aatacctccc tctggaaaat ggaatgaaag ccgaaagtac actatttctg     1320

gaaaaaaggc gtatttacca gtggatgctt tctcctttct atgacatgaa acacagtgca     1380

acaggaccga tcaatgacta acaggaattt cagacaaatt ataaatctgc ttgatttgcg     1440

ctggcaacgt cgtgttccgg ttattcatca gacggagacc gctgaatgtg gactggcctg     1500

cctagcaatg atatgcggtc attttggtaa gaatattgac ctgatatatc ttcgccggaa     1560

gtttaatctc tctgcccgtg gagcaaccct tgcaggaatc aatggaatag cggagcaact     1620

ggggatggcc acccgggctc tttcactgga gttggatgaa cttcgagtcc tcaaaacgcc     1680

gtgtattctc cactgggatt tcagtcactt cgtcgttctg gtcagcgtaa agcgtaaccg     1740

ttatgtactg catgatccgg ccaggggcat aagatatatc agccgggagg aaatgagccg     1800

atattttaca ggcgttgcac ttgaggtctg gcccggaagt gaattccagt cggaaaccct     1860

gcagacccgc ataagtcttc gttcactgat taacagtatt tacggtatta aaagaacgct     1920

ggcgaaaatt ttctgtctgt cagttgtaat tgaagcaatc aatctgctaa tgccggtggg     1980

gacacagctg gttatggatc atgctattcc tgcgggggac agagggctac tgacgctaat     2040

ttctgctgct cttatgtttt ttatattact caaagctgca acgagtacgc tgcgcgcatg     2100

gtcttcactg gttatgagca cgctcatcaa tgtacagtgg cagtcggggc tgttcgatca     2160

tcttctcaga ctaccgctgg cgttttttga acgccgaaaa ttaggtgata tccagtcacg     2220

ttttgactcc cttgacacat tgagggccac atttaccacc agtgtgatcg ggttcataat     2280

ggacagcatt atggttgtcg gtgtttgtgt gatgatgctg ttatacggag gatatctcac     2340

ctggatagtt ctctgcttta ccacaattta catttttatt cgactggtga catacggcaa     2400

ttaccgacag atatcagaag aatgtcttgt cagggaggcc cgtgccgcct cctattttat     2460

ggaaacatta tatggtattg ccacggtaaa aatccagggg atggtcggaa ttcggggggc     2520

acactggctt aatatgaaaa tagatgcgat aaattcgggt attaagctaa ccaggatgga     2580

tttgctcttc ggaggaataa atacctttgt taccgcctgt gatcagattg taattttatg     2640

gctgggagca ggccttgtga tcgataatca gatgacaata ggaatgtttg tagcgtttag     2700

ttcttttcgt gggcagtttt cggaaagagt tgcctctctg accagttttc ttcttcagct     2760

aagaataatg agtctgcaca atgagcgcat tgcagatatt gcattacatg aaaaggagga     2820

aaagaaacct gaaattgaaa tcgttgctga tatggggcca atatccctgg aaaccaatgg     2880

tttaagctat cgttatgaca gtcagtcagc accgatattc agtgctctga gtttatctgt     2940

agctccgggg gaaagtgtgg ctataactgg tgcttccggt gcgggaaaaa ccacattaat     3000

gaaagtacta tgtggactat ttgaacctga tagcgggagg gtactgataa atggtataga     3060

tatacgccaa attggaataa ataattatca ccggatgata gcctgtgtta tgcaggatga     3120

ccggctattt tcaggctcaa ttcgtgaaaa tatctgtggt tttgcagagg aaatggatga     3180

agagtggatg gtagaatgtg ccagagcaag tcatattcat gatgttataa tgaatatgcc     3240

aatgggatat gaaacattaa taggtgaact tggggaaggt ctttctggcg gtcaaaaaca     3300

gcgtatattt attgcacgag ccttataccg gaaaccagga atattattta tggatgaggc     3360

aaccagtgct cttgattcag agagtgaaca tttcgtgaat gttgccataa aaaacatgaa     3420

tatcaccagg gtaattattg cacacagaga aacaacgttg agaactgttg atagagttat     3480

ttctatttaa accatagagg aattacaagc gtatgaggaa tatttcttcc tgttataatt     3540

cctcgttatg ctcagatatc tgttggaggt ggaatggaag atagacaatc caccaagaag     3600

aaatatcatt ctgtgtggat tgtccaataa ct                                   3632


<210>  3
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of synthetic promoter J23109

<400>  3
tttacagcta gctcagtcct agggactgtg ctagc                                  35


<210>  4
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of synthetic promoter J23106

<400>  4
tttacggcta gctcagtcct aggtatagtg ctagc                                  35


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of synthetic promoter J23100

<400>  5
ttgacggcta gctcagtcct aggtacagtg ctagc                                  35


<210>  6
<211>  150
<212>  DNA
<213>  Escherichia coli

<400>  6
agttgttctt attggtggtg ttgctttatg gttgcatcgt agtaaatggt tgtaacaaaa       60

gcaatttttc cggctgtctg tatacaaaaa cgccgtaaag tttgagcgaa gtcaataaac      120

tctctaccca ttcagggcaa tatctctctt                                       150


<210>  7
<211>  174
<212>  DNA
<213>  Escherichia coli

<400>  7
gatcgcccga acagcaatgt ttgggcgatt tttattacga taataaagtc tgtttttaat       60

attatcatgt taaatgttta tattataaaa agtcgttttt ctgcttagga ttttgttatt      120

taaattaagc ctgtaatgcc ttgcttccat tgcggataaa tcctactttt ttat            174


<210>  8
<211>  242
<212>  DNA
<213>  Escherichia coli

<400>  8
tcaagtacta atagtgatat tttaaggtct gatttttacg tgataattca ggagacacag       60

aatgcgcata aaaataacag cataaaacac cttaccacca cccaagaatt tcatattgta      120

ttgtttttca atgaaaaaat attattcgcg taatatctca cgataaataa cattaggatt      180

ttgttattta aacacgagtc ctttgcactt gcttacttta tcgataaatc ctactttttt      240

aa                                                                     242


<210>  9
<211>  63
<212>  DNA
<213>  Escherichia coli

<400>  9
ccctgcgcgc gagcagattt cacggaataa tttcaccaga cttattctta gctattatag       60

tta                                                                     63


<210>  10
<211>  260
<212>  DNA
<213>  Escherichia coli

<400>  10
ttttttctta attgatggct aaatattctg aaataattag aaaaatgtat aaaaatccaa       60

aatattgtac taaatttgac cacttttgca gattgattag tttatggatg tttgtatcta      120

aatgatttta ttgataaatt actaaagcgt aatgattatt gatctcaatt gtattttgtg      180

ctaataaaat tctaacagaa ggacgtgagg ttcctctgta aaaatcatca tactatttcc      240

atcaaataag gaacgtaaaa                                                  260


<210>  11
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of synthetic RBS of bicistronic design by Mutalik et
       al.

<400>  11
gggcccaagt tcacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc       60

ttaatcatgc taaggaggtt ttct                                              84


<210>  12
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of synthetic transcriptional terminator

<400>  12
ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt       60

gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct      120

gcgtttata                                                              129


<210>  13
<211>  6141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of vector required for Enterocin A production and 
       secretion from Escherichia coli

<400>  13
atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg       60

aattcggtct ctggagttga cggctagctc agtcctaggt acagtgctag ctacttacat      120

aacaacagca acaactaagg aggttttcaa tgcgcactct gactctgaat gaattagatt      180

ctgtttctgg tggtaccact catagcggta agtattacgg aaatggagtt tactgtacca      240

aaaataaatg caccgttgat tgggctaaag cgacaacttg tatcgctggt atgtctatcg      300

gcgggttctt agggggtgcc attccaggca aatgctaagc ttggtcgaag ctcagaggat      360

cgtacaggct tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg      420

ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg      480

tgggcctttc tgcgtttata cgctcagagg agcctgataa ctctcctatg ttgtatgttt      540

atatgatttt ccttgaaaca tataatgcaa attttcgatt tattttccat cattaatcca      600

gataaacaac aaactaatag tatgcaagga gacattattt gtttcgccat gatgctttag      660

aaaacagaaa aatgaagtgg cagggacggg caatattact tcccggaata ccactgtggt      720

taatcatgct gggaagcatt gtgtttatta cggcatttct gatgttcatt attgttggta      780

cctatagccg ccgtgttaat gtcagtggtg aggtcacaac ctggccaaga gctgtcaata      840

tatattcagg tgtacaggga tttgttgtca ggcagtttgt tcatgaaggg cagttgataa      900

aaaaagggga tcctgtttat ctgattgaca tcagtaaaag tacacgcaat ggtattgtca      960

ctgataatca tcgccgggat atagaaaacc agctggttcg tgtggacaac attatttccc     1020

gtctggaaga aagtaaaaaa ataacgctag ataccctgga aaaacaacgt ctgcaataca     1080

cagatgcgtt ccgtcgctca tcagacatta tacagcgtgc agaggaaggg ataaaaataa     1140

tgaaaaataa tatggagaat tacagatact atcagtcaaa aggactgatt aataaagatc     1200

aattaactaa ccaagttgca ttatattatc aacaacaaaa caaccttctc agtctgagcg     1260

gacaaaatga acaaaatgcc ctgcagataa ccactctgga gagtcagatt cagactcagg     1320

cagcagattt tgataatcgt atctatcaga tggaactgca acgactcgaa ttgcagaaag     1380

aactggttaa cactgatgtg gaaggcgaaa tcattatccg ggcgttgtct gacgggaaag     1440

ttgactccct gagtgtcact gtagggcaaa tggtcaatac cggagacagc cttctgcagg     1500

ttattcctga gaacattgaa aactattatc ttattctctg ggtcccgaat gatgctgttc     1560

cttatatttc ggctggtgac aaagtgaata ttcgttatga agccttcccc tcagaaaaat     1620

ttgggcagtt ctctgctacg gttaaaacta tatccaggac tcctgcgtca acacaggaaa     1680

tgttgaccta taagggagca cctcaaaata cgccgggtgc ctctgttccc tggtataaag     1740

tcattgcgac gcctgaaaag cagataatca ggtatgacga aaaatacctc cctctggaaa     1800

atggaatgaa agccgaaagt acactatttc tggaaaaaag gcgtatttac cagtggatgc     1860

tttctccttt ctatgacatg aaacacagtg caacaggacc gatcaatgac taacaggaat     1920

ttcagacaaa ttataaatct gcttgatttg cgctggcaac gtcgtgttcc ggttattcat     1980

cagacggaga ccgctgaatg tggactggcc tgcctagcaa tgatatgcgg tcattttggt     2040

aagaatattg acctgatata tcttcgccgg aagtttaatc tctctgcccg tggagcaacc     2100

cttgcaggaa tcaatggaat agcggagcaa ctggggatgg ccacccgggc tctttcactg     2160

gagttggatg aacttcgagt cctcaaaacg ccgtgtattc tccactggga tttcagtcac     2220

ttcgtcgttc tggtcagcgt aaagcgtaac cgttatgtac tgcatgatcc ggccaggggc     2280

ataagatata tcagccggga ggaaatgagc cgatatttta caggcgttgc acttgaggtc     2340

tggcccggaa gtgaattcca gtcggaaacc ctgcagaccc gcataagtct tcgttcactg     2400

attaacagta tttacggtat taaaagaacg ctggcgaaaa ttttctgtct gtcagttgta     2460

attgaagcaa tcaatctgct aatgccggtg gggacacagc tggttatgga tcatgctatt     2520

cctgcggggg acagagggct actgacgcta atttctgctg ctcttatgtt ttttatatta     2580

ctcaaagctg caacgagtac gctgcgcgca tggtcttcac tggttatgag cacgctcatc     2640

aatgtacagt ggcagtcggg gctgttcgat catcttctca gactaccgct ggcgtttttt     2700

gaacgccgaa aattaggtga tatccagtca cgttttgact cccttgacac attgagggcc     2760

acatttacca ccagtgtgat cgggttcata atggacagca ttatggttgt cggtgtttgt     2820

gtgatgatgc tgttatacgg aggatatctc acctggatag ttctctgctt taccacaatt     2880

tacattttta ttcgactggt gacatacggc aattaccgac agatatcaga agaatgtctt     2940

gtcagggagg cccgtgccgc ctcctatttt atggaaacat tatatggtat tgccacggta     3000

aaaatccagg ggatggtcgg aattcggggg gcacactggc ttaatatgaa aatagatgcg     3060

ataaattcgg gtattaagct aaccaggatg gatttgctct tcggaggaat aaataccttt     3120

gttaccgcct gtgatcagat tgtaatttta tggctgggag caggccttgt gatcgataat     3180

cagatgacaa taggaatgtt tgtagcgttt agttcttttc gtgggcagtt ttcggaaaga     3240

gttgcctctc tgaccagttt tcttcttcag ctaagaataa tgagtctgca caatgagcgc     3300

attgcagata ttgcattaca tgaaaaggag gaaaagaaac ctgaaattga aatcgttgct     3360

gatatggggc caatatccct ggaaaccaat ggtttaagct atcgttatga cagtcagtca     3420

gcaccgatat tcagtgctct gagtttatct gtagctccgg gggaaagtgt ggctataact     3480

ggtgcttccg gtgcgggaaa aaccacatta atgaaagtac tatgtggact atttgaacct     3540

gatagcggga gggtactgat aaatggtata gatatacgcc aaattggaat aaataattat     3600

caccggatga tagcctgtgt tatgcaggat gaccggctat tttcaggctc aattcgtgaa     3660

aatatctgtg gttttgcaga ggaaatggat gaagagtgga tggtagaatg tgccagagca     3720

agtcatattc atgatgttat aatgaatatg ccaatgggat atgaaacatt aataggtgaa     3780

cttggggaag gtctttctgg cggtcaaaaa cagcgtatat ttattgcacg agccttatac     3840

cggaaaccag gaatattatt tatggatgag gcaaccagtg ctcttgattc agagagtgaa     3900

catttcgtga atgttgccat aaaaaacatg aatatcacca gggtaattat tgcacacaga     3960

gaaacaacgt tgagaactgt tgatagagtt atttctattt aaaccataga ggaattacaa     4020

gcgtatgagg aatatttctt cctgttataa ttcctcgtta tgctcagata tctgttggag     4080

gtggaatgga agatagacaa tccaccaaga agaaatatca ttctgtgtgg attgtccaat     4140

aactcgctgt cacgcttgag accacccctg cagtccggca aaaaagggca aggtgtcacc     4200

accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct     4260

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     4320

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     4380

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg     4440

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     4500

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     4560

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     4620

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     4680

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     4740

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     4800

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     4860

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     4920

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     4980

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     5040

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     5100

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     5160

atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg     5220

gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat     5280

caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt     5340

actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa     5400

cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga     5460

aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca     5520

cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca     5580

ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt     5640

cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac     5700

aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg     5760

gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat     5820

ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac     5880

attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa     5940

cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg     6000

ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc     6060

ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca     6120

ttaacctata aaaataggcg t                                               6141


<210>  14
<211>  47
<212>  PRT
<213>  Enterococcus faecium

<400>  14

Thr Thr His Ser Gly Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Thr Lys 
1               5                   10                  15      


Asn Lys Cys Thr Val Asp Trp Ala Lys Ala Thr Thr Cys Ile Ala Gly 
            20                  25                  30          


Met Ser Ile Gly Gly Phe Leu Gly Gly Ala Ile Pro Gly Lys Cys 
        35                  40                  45          


<210>  15
<211>  44
<212>  PRT
<213>  Enterococcus faecium

<400>  15

Ala Thr Arg Ser Tyr Gly Asn Gly Val Tyr Cys Asn Asn Ser Lys Cys 
1               5                   10                  15      


Trp Val Asn Trp Gly Glu Ala Lys Glu Asn Ile Ala Gly Ile Val Ile 
            20                  25                  30          


Ser Gly Trp Ala Ser Gly Leu Ala Gly Met Gly His 
        35                  40                  


<210>  16
<211>  53
<212>  PRT
<213>  Enterococcus faecium

<400>  16

Glu Asn Asp His Arg Met Pro Asn Glu Leu Asn Arg Pro Asn Asn Leu 
1               5                   10                  15      


Ser Lys Gly Gly Ala Lys Cys Gly Ala Ala Ile Ala Gly Gly Leu Phe 
            20                  25                  30          


Gly Ile Pro Lys Gly Pro Leu Ala Trp Ala Ala Gly Leu Ala Asn Val 
        35                  40                  45              


Tyr Ser Lys Cys Asn 
    50              


<210>  17
<211>  44
<212>  PRT
<213>  Enterococcus hirae

<400>  17

Ala Thr Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Glu Lys Cys Trp 
1               5                   10                  15      


Val Asp Trp Asn Gln Ala Lys Gly Glu Ile Gly Lys Ile Ile Val Asn 
            20                  25                  30          


Gly Trp Val Asn His Gly Pro Trp Ala Pro Arg Arg 
        35                  40                  


<210>  18
<211>  289
<212>  PRT
<213>  Enterococcus faecalis

<400>  18

Met Ala Gly Glu Val Phe Ser Ser Leu Ile Thr Ser Val Asn Pro Asn 
1               5                   10                  15      


Pro Met Asn Ala Gly Ser Arg Asn Gly Ile Pro Ile Asp Thr Ile Ile 
            20                  25                  30          


Leu His His Asn Ala Thr Thr Asn Lys Asp Val Ala Met Asn Thr Trp 
        35                  40                  45              


Leu Leu Gly Gly Gly Ala Gly Thr Ser Ala His Tyr Glu Cys Thr Pro 
    50                  55                  60                  


Thr Glu Ile Ile Gly Cys Val Gly Glu Gln Tyr Ser Ala Phe His Ala 
65                  70                  75                  80  


Gly Gly Thr Gly Gly Ile Asp Val Pro Lys Ile Ala Asn Pro Asn Gln 
                85                  90                  95      


Arg Ser Ile Gly Ile Glu Asn Val Asn Ser Ser Gly Ala Pro Asn Trp 
            100                 105                 110         


Ser Val Asp Pro Arg Thr Ile Thr Asn Cys Ala Arg Leu Val Ala Asp 
        115                 120                 125             


Ile Cys Thr Arg Tyr Gly Ile Pro Cys Asp Arg Gln His Val Leu Gly 
    130                 135                 140                 


His Asn Glu Val Thr Ala Thr Ala Cys Pro Gly Gly Met Asp Val Asp 
145                 150                 155                 160 


Glu Val Val Arg Gln Ala Gln Gln Phe Met Ala Gly Gly Ser Asn Asn 
                165                 170                 175     


Ala Val Lys Pro Glu Pro Ser Lys Pro Thr Pro Ser Lys Pro Ser Asn 
            180                 185                 190         


Asn Lys Asn Lys Glu Gly Val Ala Thr Met Tyr Cys Leu Tyr Glu Arg 
        195                 200                 205             


Pro Ile Asn Ser Lys Thr Gly Val Leu Glu Trp Asn Gly Asp Ala Trp 
    210                 215                 220                 


Thr Val Met Phe Cys Asn Gly Val Asn Cys Arg Arg Val Ser His Pro 
225                 230                 235                 240 


Asp Glu Met Lys Val Ile Glu Asp Ile Tyr Arg Lys Asn Asn Gly Lys 
                245                 250                 255     


Asp Ile Pro Phe Tyr Ser Gln Lys Glu Trp Asn Lys Asn Ala Pro Trp 
            260                 265                 270         


Tyr Asn Arg Leu Glu Thr Val Cys Pro Val Val Gly Ile Thr Lys Lys 
        275                 280                 285             


Ser 
    


<210>  19
<211>  410
<212>  PRT
<213>  phage F1

<400>  19

Met Ser Asn Ile Asn Met Glu Thr Ala Ile Ala Asn Met Tyr Ala Leu 
1               5                   10                  15      


Lys Ala Arg Gly Ile Thr Tyr Ser Met Asn Tyr Ser Arg Thr Gly Ala 
            20                  25                  30          


Asp Gly Thr Gly Asp Cys Ser Gly Thr Val Tyr Asp Ser Leu Arg Lys 
        35                  40                  45              


Ala Gly Ala Ser Asp Ala Gly Trp Val Leu Asn Thr Asp Ser Met His 
    50                  55                  60                  


Ser Trp Leu Glu Lys Asn Gly Phe Lys Leu Ile Ala Gln Asn Lys Glu 
65                  70                  75                  80  


Trp Ser Ala Lys Arg Gly Asp Val Val Ile Phe Gly Lys Lys Gly Ala 
                85                  90                  95      


Ser Gly Gly Ser Ala Gly His Val Val Ile Phe Ile Ser Ser Thr Gln 
            100                 105                 110         


Ile Ile His Cys Thr Trp Lys Ser Ala Thr Ala Asn Gly Val Tyr Val 
        115                 120                 125             


Asp Asn Glu Ala Thr Thr Cys Pro Tyr Ser Met Gly Trp Tyr Val Tyr 
    130                 135                 140                 


Arg Leu Asn Gly Gly Ser Thr Pro Pro Lys Pro Asn Thr Lys Lys Val 
145                 150                 155                 160 


Lys Val Leu Lys His Ala Thr Asn Trp Ser Pro Ser Ser Lys Gly Ala 
                165                 170                 175     


Lys Met Ala Ser Phe Val Lys Gly Gly Thr Phe Glu Val Lys Gln Gln 
            180                 185                 190         


Arg Pro Ile Ser Tyr Ser Tyr Ser Asn Gln Glu Tyr Leu Ile Val Asn 
        195                 200                 205             


Lys Gly Thr Val Leu Gly Trp Val Leu Ser Gln Asp Ile Glu Gly Gly 
    210                 215                 220                 


Tyr Gly Ser Asp Arg Val Gly Gly Ser Lys Pro Lys Leu Pro Ala Gly 
225                 230                 235                 240 


Phe Thr Lys Glu Glu Ala Thr Phe Ile Asn Gly Asn Ala Pro Ile Thr 
                245                 250                 255     


Thr Arg Lys Asn Lys Pro Ser Leu Ser Ser Gln Thr Ala Thr Pro Leu 
            260                 265                 270         


Tyr Pro Gly Gln Ser Val Arg Tyr Leu Gly Trp Lys Ser Ala Glu Gly 
        275                 280                 285             


Tyr Ile Trp Ile Tyr Ala Thr Asp Gly Arg Tyr Ile Pro Val Arg Pro 
    290                 295                 300                 


Val Gly Lys Glu Ala Trp Gly Thr Phe Lys Gln Asp Ile Glu Gly Gly 
305                 310                 315                 320 


Tyr Gly Ser Asp Arg Val Gly Gly Ser Lys Pro Lys Leu Pro Ala Gly 
                325                 330                 335     


Phe Thr Lys Glu Glu Ala Thr Phe Ile Asn Gly Asn Ala Pro Ile Thr 
            340                 345                 350         


Thr Arg Lys Asn Lys Pro Ser Leu Ser Ser Gln Thr Ala Thr Pro Leu 
        355                 360                 365             


Tyr Pro Gly Gln Ser Val Arg Tyr Leu Gly Trp Lys Ser Ala Glu Gly 
    370                 375                 380                 


Tyr Ile Trp Ile Tyr Ala Thr Asp Gly Arg Tyr Ile Pro Val Arg Pro 
385                 390                 395                 400 


Val Gly Lys Glu Ala Trp Gly Thr Phe Lys 
                405                 410 


<210>  20
<211>  328
<212>  PRT
<213>  phage EFAP-1

<400>  20

Met Lys Leu Lys Gly Ile Leu Leu Ser Val Val Thr Thr Phe Gly Leu 
1               5                   10                  15      


Leu Phe Gly Ala Thr Asn Val Gln Ala Tyr Glu Val Asn Asn Glu Phe 
            20                  25                  30          


Asn Leu Gln Pro Trp Glu Gly Ser Gln Gln Leu Ala Tyr Pro Asn Lys 
        35                  40                  45              


Ile Ile Leu His Glu Thr Ala Asn Pro Arg Ala Thr Gly Arg Asn Glu 
    50                  55                  60                  


Ala Thr Tyr Met Lys Asn Asn Trp Phe Asn Ala His Thr Thr Ala Ile 
65                  70                  75                  80  


Val Gly Asp Gly Gly Ile Val Tyr Lys Val Ala Pro Glu Gly Asn Val 
                85                  90                  95      


Ser Trp Gly Ala Gly Asn Ala Asn Pro Tyr Ala Pro Val Gln Ile Glu 
            100                 105                 110         


Leu Gln His Thr Asn Asp Pro Glu Leu Phe Lys Ala Asn Tyr Lys Ala 
        115                 120                 125             


Tyr Val Asp Tyr Thr Arg Asp Met Gly Lys Lys Phe Gly Ile Pro Met 
    130                 135                 140                 


Thr Leu Asp Gln Gly Gly Ser Leu Trp Glu Lys Gly Val Val Ser His 
145                 150                 155                 160 


Gln Trp Val Thr Asp Phe Val Trp Gly Asp His Thr Asp Pro Tyr Gly 
                165                 170                 175     


Tyr Leu Ala Lys Met Gly Ile Ser Lys Ala Gln Leu Ala His Asp Leu 
            180                 185                 190         


Ala Asn Gly Val Ser Gly Asn Thr Ala Thr Pro Thr Pro Lys Pro Asp 
        195                 200                 205             


Lys Pro Lys Pro Thr Gln Pro Ser Lys Pro Ser Asn Lys Lys Arg Phe 
    210                 215                 220                 


Asn Tyr Arg Val Asp Gly Leu Glu Tyr Val Asn Gly Met Trp Gln Ile 
225                 230                 235                 240 


Tyr Asn Glu His Leu Gly Lys Ile Asp Phe Asn Trp Thr Glu Asn Gly 
                245                 250                 255     


Ile Pro Val Glu Val Val Asp Lys Val Asn Pro Ala Thr Gly Gln Pro 
            260                 265                 270         


Thr Lys Asp Gln Val Leu Lys Val Gly Asp Tyr Phe Asn Phe Gln Glu 
        275                 280                 285             


Asn Ser Thr Gly Val Val Gln Glu Gln Thr Pro Tyr Met Gly Tyr Thr 
    290                 295                 300                 


Leu Ser His Val Gln Leu Pro Asn Glu Phe Ile Trp Leu Phe Thr Asp 
305                 310                 315                 320 


Ser Lys Gln Ala Leu Met Tyr Gln 
                325             


<210>  21
<211>  289
<212>  PRT
<213>  phage jEF24C

<400>  21

Met Ala Gly Glu Val Phe Ser Ser Leu Ile Thr Ser Val Asn Pro Asn 
1               5                   10                  15      


Pro Met Asn Ala Gly Ser Arg Asn Gly Ile Pro Ile Asp Thr Ile Ile 
            20                  25                  30          


Leu His His Asn Ala Thr Thr Asn Lys Asp Val Ala Met Asn Thr Trp 
        35                  40                  45              


Leu Leu Gly Gly Gly Ala Gly Thr Ser Ala His Tyr Glu Cys Thr Pro 
    50                  55                  60                  


Thr Glu Ile Ile Gly Cys Val Gly Glu Gln Tyr Ser Ala Phe His Ala 
65                  70                  75                  80  


Gly Gly Thr Gly Gly Ile Asp Val Pro Lys Ile Ala Asn Pro Asn Gln 
                85                  90                  95      


Arg Ser Ile Gly Ile Glu Asn Val Asn Ser Ser Gly Ala Pro Asn Trp 
            100                 105                 110         


Ser Val Asp Pro Arg Thr Ile Thr Asn Cys Ala Arg Leu Val Ala Asp 
        115                 120                 125             


Ile Cys Thr Arg Tyr Gly Ile Pro Cys Asp Arg Gln His Val Leu Gly 
    130                 135                 140                 


His Asn Glu Val Thr Ala Thr Ala Cys Pro Gly Gly Met Asp Val Asp 
145                 150                 155                 160 


Glu Val Val Arg Gln Ala Gln Gln Phe Met Ala Gly Gly Ser Asn Asn 
                165                 170                 175     


Ala Val Lys Pro Glu Pro Ser Lys Pro Thr Pro Ser Lys Pro Ser Asn 
            180                 185                 190         


Asn Lys Asn Lys Glu Gly Val Ala Thr Met Tyr Cys Leu Tyr Glu Arg 
        195                 200                 205             


Pro Ile Asn Ser Lys Thr Gly Val Leu Glu Trp Asn Gly Asp Ala Trp 
    210                 215                 220                 


Thr Val Met Phe Cys Asn Gly Val Asn Cys Arg Arg Val Ser His Pro 
225                 230                 235                 240 


Asp Glu Met Lys Val Ile Glu Asp Ile Tyr Arg Lys Asn Asn Gly Lys 
                245                 250                 255     


Asp Ile Pro Phe Tyr Ser Gln Lys Glu Trp Asn Lys Asn Ala Pro Trp 
            260                 265                 270         


Tyr Asn Arg Leu Glu Thr Val Cys Pro Val Val Gly Ile Thr Lys Lys 
        275                 280                 285             


Ser 
    


<210>  22
<211>  237
<212>  PRT
<213>  phage F168/08

<400>  22

Met Val Lys Leu Asn Asp Val Leu Ser Tyr Val Asn Gly Leu Val Gly 
1               5                   10                  15      


Lys Gly Val Asp Ala Asp Gly Trp Tyr Gly Thr Gln Cys Met Asp Leu 
            20                  25                  30          


Thr Val Asp Val Met Gln Arg Phe Phe Gly Trp Arg Pro Tyr Gly Asn 
        35                  40                  45              


Ala Ile Ala Leu Val Asp Gln Pro Ile Pro Ala Gly Phe Gln Arg Ile 
    50                  55                  60                  


Arg Thr Thr Ser Ser Thr Gln Ile Lys Ala Gly Asp Val Met Ile Trp 
65                  70                  75                  80  


Gly Leu Gly Tyr Tyr Ala Gln Tyr Gly His Thr His Ile Ala Thr Glu 
                85                  90                  95      


Asp Gly Arg Ala Asp Gly Thr Phe Val Ser Val Asp Gln Asn Trp Ile 
            100                 105                 110         


Asn Pro Ser Leu Glu Val Gly Ser Pro Ala Ala Ala Ile His His Asn 
        115                 120                 125             


Met Asp Gly Val Trp Gly Val Ile Arg Pro Pro Tyr Glu Ala Glu Ser 
    130                 135                 140                 


Lys Pro Lys Pro Pro Ala Pro Lys Pro Asp Lys Pro Asn Leu Gly Gln 
145                 150                 155                 160 


Phe Lys Gly Asp Asp Asp Ile Met Phe Ile Tyr Tyr Lys Lys Thr Lys 
                165                 170                 175     


Gln Gly Ser Thr Glu Gln Trp Phe Val Ile Gly Gly Lys Arg Ile Tyr 
            180                 185                 190         


Leu Pro Thr Met Thr Tyr Val Asn Glu Ala Asn Asp Leu Ile Lys Arg 
        195                 200                 205             


Tyr Gly Gly Asn Thr Asn Val Thr Thr Tyr Asn Tyr Asp Asn Phe Gly 
    210                 215                 220                 


Leu Ala Met Met Glu Lys Ala Tyr Pro Gln Val Lys Leu 
225                 230                 235         


<210>  23
<211>  25
<212>  PRT
<213>  Lactobacillus plantarum

<400>  23

Gly Ala Trp Lys Asn Phe Trp Ser Ser Leu Arg Lys Gly Phe Tyr Asp 
1               5                   10                  15      


Gly Glu Ala Gly Arg Ala Ile Arg Arg 
            20                  25  


<210>  24
<211>  32
<212>  PRT
<213>  Lactobacillus plantarum

<400>  24

Arg Arg Ser Arg Lys Asn Gly Ile Gly Tyr Ala Ile Gly Tyr Ala Phe 
1               5                   10                  15      


Gly Ala Val Glu Arg Ala Val Leu Gly Gly Ser Arg Asp Tyr Asn Lys 
            20                  25                  30          


<210>  25
<211>  33
<212>  PRT
<213>  Lactobacillus plantarum

<400>  25

Phe Asn Arg Gly Gly Tyr Asn Phe Gly Lys Ser Val Arg His Val Val 
1               5                   10                  15      


Asp Ala Ile Gly Ser Val Ala Gly Ile Arg Gly Ile Leu Lys Ser Ile 
            20                  25                  30          


Arg 
    


<210>  26
<211>  34
<212>  PRT
<213>  Lactobacillus plantarum

<400>  26

Val Phe His Ala Tyr Ser Ala Arg Gly Val Arg Asn Asn Tyr Lys Ser 
1               5                   10                  15      


Ala Val Gly Pro Ala Asp Trp Val Ile Ser Ala Val Arg Gly Phe Ile 
            20                  25                  30          


His Gly 
        


<210>  27
<211>  75
<212>  PRT
<213>  Escherichia coli

<400>  27

Ala Gly Asp Pro Leu Ala Asp Pro Asn Ser Gln Ile Val Arg Gln Ile 
1               5                   10                  15      


Met Ser Asn Ala Ala Trp Gly Ala Ala Phe Gly Ala Arg Gly Gly Leu 
            20                  25                  30          


Gly Gly Met Ala Val Gly Ala Ala Gly Gly Val Thr Gln Thr Val Leu 
        35                  40                  45              


Gln Gly Ala Ala Ala His Met Pro Val Asn Val Pro Ile Pro Lys Val 
    50                  55                  60                  


Pro Met Gly Pro Ser Trp Asn Gly Ser Lys Gly 
65                  70                  75  


<210>  28
<211>  19
<212>  DNA
<213>  Escherichia coli

<400>  28
gatcgcccga acagcaatg                                                    19


<210>  29
<211>  23
<212>  DNA
<213>  Escherichia coli

<400>  29
ataaaaaagt aggatttatc cgc                                               23


<210>  30
<211>  27
<212>  DNA
<213>  Escherichia coli

<400>  30
tcaagtacta atagtgatat tttaagg                                           27


<210>  31
<211>  27
<212>  DNA
<213>  Escherichia coli

<400>  31
ttaaaaaagt aggatttatc gataaag                                           27


<210>  32
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  32
cacacacaaa cgggagctgt t                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  33
cttcccgcag catagttcca t                                                 21


<210>  34
<211>  22
<212>  DNA
<213>  Escherichia coli

<400>  34
aagtcaaagc aggggttgcc cg                                                22


<210>  35
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  35
gacgccgaca ttaagacgca g                                                 21


<210>  36
<211>  28
<212>  DNA
<213>  Escherichia coli

<400>  36
tcatcccgga agcctccctc actactat                                          28


<210>  37
<211>  28
<212>  DNA
<213>  Escherichia coli

<400>  37
tagcgtttgc tgcactggct tctgatac                                          28


<210>  38
<211>  24
<212>  DNA
<213>  Escherichia coli

<400>  38
ggcgatttag gcattccgat actc                                              24


<210>  39
<211>  20
<212>  DNA
<213>  Escherichia coli

<400>  39
acggggtcgc tagttaagga                                                   20


<210>  40
<211>  26
<212>  DNA
<213>  Escherichia coli

<400>  40
cagcagcgct tcggacaaaa tctcct                                            26


<210>  41
<211>  26
<212>  DNA
<213>  Escherichia coli

<400>  41
ttccccacca ctctccgttc tcaaac                                            26


<210>  42
<211>  22
<212>  DNA
<213>  Escherichia coli

<400>  42
cagcaacccg aaccacttga tg                                                22


<210>  43
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  43
agcattgcca gagcggcaga a                                                 21


<210>  44
<211>  22
<212>  DNA
<213>  Escherichia coli

<400>  44
ggctggacat catgggaact gg                                                22


<210>  45
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  45
cgtcgggaac gggtagaatc g                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Escherichia coli

<400>  46
gatgactcag ccacgggtaa                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Escherichia coli

<400>  47
ccaggactca cctcacgaat                                                   20


<210>  48
<211>  25
<212>  DNA
<213>  Escherichia coli

<400>  48
gtggcagtat gagtaatgac cgtta                                             25


<210>  49
<211>  25
<212>  DNA
<213>  Escherichia coli

<400>  49
atatcctttc tgcagggatg caata                                             25


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Class IIa bacteriocin N-terminal consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be Valine or Lysine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid.

<400>  50

Tyr Gly Asn Gly Xaa Xaa Cys 
1               5           


