                         SEQUENCE LISTING

<110>  Purdue Research Foundation
 
<120>  LIGAND-MEDIATED DELIVERY OF THERAPEUTIC PROTEINS AND THE USES 
       THEREOF

<130>  68875-02

<150>  US 62/967,767
<151>  2020-01-30

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  821
<212>  PRT
<213>  artificial sequence

<220>
<223>  S7 or pepL

<400>  1

Met Glu Met Pro Gly Arg Arg Ser Asn Tyr Thr Leu Leu Ser Gln Phe 
1               5                   10                  15      


Ser Asp Asp Gln Val Ser Val Ser Val Thr Gly Ala Pro Pro Pro His 
            20                  25                  30          


Tyr Asp Ser Leu Ser Ser Glu Asn Arg Ser Asn His Asn Ser Gly Asn 
        35                  40                  45              


Thr Gly Lys Ala Lys Ala Glu Arg Gly Gly Phe Asp Trp Asp Pro Ser 
    50                  55                  60                  


Gly Gly Gly Gly Gly Asp His Arg Leu Asn Asn Gln Pro Asn Arg Val 
65                  70                  75                  80  


Gly Asn Asn Met Tyr Ala Ser Ser Leu Gly Leu Gln Arg Gln Ser Ser 
                85                  90                  95      


Gly Ser Ser Phe Gly Glu Ser Ser Leu Ser Gly Asp Tyr Tyr Met Pro 
            100                 105                 110         


Thr Leu Ser Ala Ala Ala Asn Glu Ile Glu Ser Val Gly Phe Pro Gln 
        115                 120                 125             


Asp Asp Gly Phe Arg Leu Gly Phe Gly Gly Gly Gly Gly Asp Leu Arg 
    130                 135                 140                 


Ile Gln Met Ala Ala Asp Ser Ala Gly Gly Ser Ser Ser Gly Lys Ser 
145                 150                 155                 160 


Trp Ala Gln Gln Thr Glu Glu Ser Tyr Gln Leu Gln Leu Ala Leu Ala 
                165                 170                 175     


Leu Arg Leu Ser Ser Glu Ala Thr Cys Ala Asp Asp Pro Asn Phe Leu 
            180                 185                 190         


Asp Pro Val Pro Asp Glu Ser Ala Leu Arg Thr Ser Pro Ser Ser Ala 
        195                 200                 205             


Glu Thr Val Ser His Arg Phe Trp Val Asn Gly Cys Leu Ser Tyr Tyr 
    210                 215                 220                 


Asp Lys Val Pro Asp Gly Phe Tyr Met Met Asn Gly Leu Asp Pro Tyr 
225                 230                 235                 240 


Ile Trp Thr Leu Cys Ile Asp Leu His Glu Ser Gly Arg Ile Pro Ser 
                245                 250                 255     


Ile Glu Ser Leu Arg Ala Val Asp Ser Gly Val Asp Ser Ser Leu Glu 
            260                 265                 270         


Ala Ile Ile Val Asp Arg Arg Ser Asp Pro Ala Phe Lys Glu Leu His 
        275                 280                 285             


Asn Arg Val His Asp Ile Ser Cys Ser Cys Ile Thr Thr Lys Glu Val 
    290                 295                 300                 


Val Asp Gln Leu Ala Lys Leu Ile Cys Asn Arg Met Gly Gly Pro Val 
305                 310                 315                 320 


Ile Met Gly Glu Asp Glu Leu Val Pro Met Trp Lys Glu Cys Ile Asp 
                325                 330                 335     


Gly Leu Lys Glu Ile Phe Lys Val Val Val Pro Ile Gly Ser Leu Ser 
            340                 345                 350         


Val Gly Leu Cys Arg His Arg Ala Leu Leu Phe Lys Val Leu Ala Asp 
        355                 360                 365             


Ile Ile Asp Leu Pro Cys Arg Ile Ala Lys Gly Cys Lys Tyr Cys Asn 
    370                 375                 380                 


Arg Asp Asp Ala Ala Ser Cys Leu Val Arg Phe Gly Leu Asp Arg Glu 
385                 390                 395                 400 


Tyr Leu Val Asp Leu Val Gly Lys Pro Gly His Leu Trp Glu Pro Asp 
                405                 410                 415     


Ser Leu Leu Asn Gly Pro Ser Ser Ile Ser Ile Ser Ser Pro Leu Arg 
            420                 425                 430         


Phe Pro Arg Pro Lys Pro Val Glu Pro Ala Val Asp Phe Arg Leu Leu 
        435                 440                 445             


Ala Lys Gln Tyr Phe Ser Asp Ser Gln Ser Leu Asn Leu Val Phe Asp 
    450                 455                 460                 


Pro Ala Ser Asp Asp Met Gly Phe Ser Met Phe His Arg Gln Tyr Asp 
465                 470                 475                 480 


Asn Pro Gly Gly Glu Asn Asp Ala Leu Ala Glu Asn Gly Gly Gly Ser 
                485                 490                 495     


Leu Pro Pro Ser Ala Asn Met Pro Pro Gln Asn Met Met Arg Ala Ser 
            500                 505                 510         


Asn Gln Ile Glu Ala Ala Pro Met Asn Ala Pro Pro Ile Ser Gln Pro 
        515                 520                 525             


Val Pro Asn Arg Ala Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met 
    530                 535                 540                 


Asp Ile Pro Trp Cys Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly 
545                 550                 555                 560 


Ser Phe Gly Thr Val His Arg Ala Glu Trp His Gly Ser Asp Val Ala 
                565                 570                 575     


Val Lys Ile Leu Met Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu 
            580                 585                 590         


Phe Leu Arg Glu Val Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile 
        595                 600                 605             


Val Leu Phe Met Gly Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val 
    610                 615                 620                 


Thr Glu Tyr Leu Ser Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser 
625                 630                 635                 640 


Gly Ala Arg Glu Gln Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr 
                645                 650                 655     


Asp Val Ala Lys Gly Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile 
            660                 665                 670         


Val His Arg Asp Leu Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr 
        675                 680                 685             


Thr Val Lys Val Cys Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr 
    690                 695                 700                 


Phe Leu Ser Ser Lys Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro 
705                 710                 715                 720 


Glu Val Leu Arg Asp Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser 
                725                 730                 735     


Phe Gly Val Ile Leu Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly 
            740                 745                 750         


Asn Leu Asn Pro Ala Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys 
        755                 760                 765             


Arg Leu Glu Ile Pro Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile 
    770                 775                 780                 


Glu Gly Cys Trp Thr Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr 
785                 790                 795                 800 


Ile Met Asp Leu Leu Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro 
                805                 810                 815     


Asn Arg Ser Asp Leu 
            820     


<210>  2
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  G4S linker

<400>  2

Gly Gly Gly Gly Ser 
1               5   


<210>  3
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Non-specific control peptide

<400>  3

Glu Asp Leu Gly Arg Glu Lys 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  rNLS oligopeptide

<400>  4

Val Lys Arg Lys Lys Lys Pro 
1               5           


<210>  5
<211>  444
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mouse IL27 with linked subunits of IL27B (EBI3) and IL27A 
       (IL27p28)

<400>  5

Met Ser Lys Leu Leu Phe Leu Ser Leu Ala Leu Trp Ala Ser Arg Ser 
1               5                   10                  15      


Pro Gly Tyr Thr Glu Thr Ala Leu Val Ala Leu Ser Gln Pro Arg Val 
            20                  25                  30          


Gln Cys His Ala Ser Arg Tyr Pro Val Ala Val Asp Cys Ser Trp Thr 
        35                  40                  45              


Pro Leu Gln Ala Pro Asn Ser Thr Arg Ser Thr Ser Phe Ile Ala Thr 
    50                  55                  60                  


Tyr Arg Leu Gly Val Ala Thr Gln Gln Gln Ser Gln Pro Cys Leu Gln 
65                  70                  75                  80  


Arg Ser Pro Gln Ala Ser Arg Cys Thr Ile Pro Asp Val His Leu Phe 
                85                  90                  95      


Ser Thr Val Pro Tyr Met Leu Asn Val Thr Ala Val His Pro Gly Gly 
            100                 105                 110         


Ala Ser Ser Ser Leu Leu Ala Phe Val Ala Glu Arg Ile Ile Lys Pro 
        115                 120                 125             


Asp Pro Pro Glu Gly Val Arg Leu Arg Thr Ala Gly Gln Arg Leu Gln 
    130                 135                 140                 


Val Leu Trp His Pro Pro Ala Ser Trp Pro Phe Pro Asp Ile Phe Ser 
145                 150                 155                 160 


Leu Lys Tyr Arg Leu Arg Tyr Arg Arg Arg Gly Ala Ser His Phe Arg 
                165                 170                 175     


Gln Val Gly Pro Ile Glu Ala Thr Thr Phe Thr Leu Arg Asn Ser Lys 
            180                 185                 190         


Pro His Ala Lys Tyr Cys Ile Gln Val Ser Ala Gln Asp Leu Thr Asp 
        195                 200                 205             


Tyr Gly Lys Pro Ser Asp Trp Ser Leu Pro Gly Gln Val Glu Ser Ala 
    210                 215                 220                 


Pro His Lys Pro Val Pro Gly Val Gly Val Pro Gly Val Gly Phe Pro 
225                 230                 235                 240 


Thr Asp Pro Leu Ser Leu Gln Glu Leu Arg Arg Glu Phe Thr Val Ser 
                245                 250                 255     


Leu Tyr Leu Ala Arg Lys Leu Leu Ser Glu Val Gln Gly Tyr Val His 
            260                 265                 270         


Ser Phe Ala Glu Ser Arg Leu Pro Gly Val Asn Leu Asp Leu Leu Pro 
        275                 280                 285             


Leu Gly Tyr His Leu Pro Asn Val Ser Leu Thr Phe Gln Ala Trp His 
    290                 295                 300                 


His Leu Ser Asp Ser Glu Arg Leu Cys Phe Leu Ala Thr Thr Leu Arg 
305                 310                 315                 320 


Pro Phe Pro Ala Met Leu Gly Gly Leu Gly Thr Gln Gly Thr Trp Thr 
                325                 330                 335     


Ser Ser Glu Arg Glu Gln Leu Trp Ala Met Arg Leu Asp Leu Arg Asp 
            340                 345                 350         


Leu His Arg His Leu Arg Phe Gln Val Leu Ala Ala Gly Phe Lys Cys 
        355                 360                 365             


Ser Lys Glu Glu Glu Asp Lys Glu Glu Glu Glu Glu Glu Glu Glu Glu 
    370                 375                 380                 


Glu Lys Lys Leu Pro Leu Gly Ala Leu Gly Gly Pro Asn Gln Val Ser 
385                 390                 395                 400 


Ser Gln Val Ser Trp Pro Gln Leu Leu Tyr Thr Tyr Gln Leu Leu His 
                405                 410                 415     


Ser Leu Glu Leu Val Leu Ser Arg Ala Val Arg Asp Leu Leu Leu Leu 
            420                 425                 430         


Ser Leu Pro Arg Arg Pro Gly Ser Ala Trp Asp Ser 
        435                 440                 


<210>  6
<211>  454
<212>  PRT
<213>  artificial sequence

<220>
<223>  human IL27 linked subunits IL27B (EBI3) and IL27A (IL27p28)

<400>  6

Met Thr Pro Gln Leu Leu Leu Ala Leu Val Leu Trp Ala Ser Cys Pro 
1               5                   10                  15      


Pro Cys Ser Gly Arg Lys Gly Pro Pro Ala Ala Leu Thr Leu Pro Arg 
            20                  25                  30          


Val Gln Cys Arg Ala Ser Arg Tyr Pro Ile Ala Val Asp Cys Ser Trp 
        35                  40                  45              


Thr Leu Pro Pro Ala Pro Asn Ser Thr Ser Pro Val Ser Phe Ile Ala 
    50                  55                  60                  


Thr Tyr Arg Leu Gly Met Ala Ala Arg Gly His Ser Trp Pro Cys Leu 
65                  70                  75                  80  


Gln Gln Thr Pro Thr Ser Thr Ser Cys Thr Ile Thr Asp Val Gln Leu 
                85                  90                  95      


Phe Ser Met Ala Pro Tyr Val Leu Asn Val Thr Ala Val His Pro Trp 
            100                 105                 110         


Gly Ser Ser Ser Ser Phe Val Pro Phe Ile Thr Glu His Ile Ile Lys 
        115                 120                 125             


Pro Asp Pro Pro Glu Gly Val Arg Leu Ser Pro Leu Ala Glu Arg Gln 
    130                 135                 140                 


Leu Gln Val Gln Trp Glu Pro Pro Gly Ser Trp Pro Phe Pro Glu Ile 
145                 150                 155                 160 


Phe Ser Leu Lys Tyr Trp Ile Arg Tyr Lys Arg Gln Gly Ala Ala Arg 
                165                 170                 175     


Phe His Arg Val Gly Pro Ile Glu Ala Thr Ser Phe Ile Leu Arg Ala 
            180                 185                 190         


Val Arg Pro Arg Ala Arg Tyr Tyr Ile Gln Val Ala Ala Gln Asp Leu 
        195                 200                 205             


Thr Asp Tyr Gly Glu Leu Ser Asp Trp Ser Leu Pro Ala Thr Ala Thr 
    210                 215                 220                 


Met Ser Leu Gly Lys Val Pro Gly Val Gly Val Pro Gly Val Gly Phe 
225                 230                 235                 240 


Pro Arg Pro Pro Gly Arg Pro Gln Leu Ser Leu Gln Glu Leu Arg Arg 
                245                 250                 255     


Glu Phe Thr Val Ser Leu His Leu Ala Arg Lys Leu Leu Ala Glu Val 
            260                 265                 270         


Arg Gly Gln Ala His Arg Phe Ala Glu Ser His Leu Pro Gly Val Asn 
        275                 280                 285             


Leu Tyr Leu Leu Pro Leu Gly Glu Gln Leu Pro Asp Val Ser Leu Thr 
    290                 295                 300                 


Phe Gln Ala Trp Arg Arg Leu Ser Asp Pro Glu Arg Leu Cys Phe Ile 
305                 310                 315                 320 


Ser Thr Thr Leu Gln Pro Phe His Ala Leu Leu Gly Gly Leu Gly Thr 
                325                 330                 335     


Gln Gly Arg Trp Thr Asn Met Glu Arg Met Gln Leu Trp Ala Met Arg 
            340                 345                 350         


Leu Asp Leu Arg Asp Leu Gln Arg His Leu Arg Phe Gln Val Leu Ala 
        355                 360                 365             


Ala Gly Phe Asn Leu Pro Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 
    370                 375                 380                 


Glu Glu Glu Arg Lys Gly Leu Leu Pro Gly Ala Leu Gly Ser Ala Leu 
385                 390                 395                 400 


Gln Gly Pro Ala Gln Val Ser Trp Pro Gln Leu Leu Ser Thr Tyr Arg 
                405                 410                 415     


Leu Leu His Ser Leu Glu Leu Val Leu Ser Arg Ala Val Arg Glu Leu 
            420                 425                 430         


Leu Leu Leu Ser Lys Ala Gly His Ser Val Trp Pro Leu Gly Phe Pro 
        435                 440                 445             


Thr Leu Ser Pro Gln Pro 
    450                 


<210>  7
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CANINE IL27 with linked subunits of IL27B (EBI3) and IL27A 
       (IL27p28)

<400>  7

Met Ala Pro Gly Leu Leu Leu Val Leu Ala Leu Trp Val Gly Cys Ser 
1               5                   10                  15      


Pro Cys Arg Gly Arg Glu Gly Ala Pro Ala Ala Pro Thr Gln Pro Arg 
            20                  25                  30          


Val Arg Cys Arg Ala Ser Arg Tyr Pro Val Ala Val Asp Cys Phe Trp 
        35                  40                  45              


Thr Leu Pro Pro Ala Pro Arg Ser Ala Thr Pro Thr Ser Phe Ile Ala 
    50                  55                  60                  


Thr Tyr Arg Leu Gly Val Ala Ala His Gly Glu Ser Leu Pro Cys Leu 
65                  70                  75                  80  


Gln Gln Thr Pro Glu Ala Thr Ser Cys Thr Ile Pro Asp Val His Met 
                85                  90                  95      


Phe Ser Met Val Pro Tyr Val Leu Asn Val Thr Ala Val Arg Pro Trp 
            100                 105                 110         


Gly Ser Ser Ser Ser Phe Val Pro Phe Val Pro Glu Gln Leu Ile Lys 
        115                 120                 125             


Pro Asp Pro Pro Glu Gly Val Arg Leu Ser Val Leu Pro Arg Gln Arg 
    130                 135                 140                 


Leu Trp Val Gln Trp Glu Pro Pro Arg Ser Trp Pro Phe Pro Glu Leu 
145                 150                 155                 160 


Phe Ser Leu Lys Tyr Trp Ile Arg Tyr Lys His His Gly Ser Pro Arg 
                165                 170                 175     


Phe Arg Gln Val Gly Pro Ile Glu Ala Thr Ser Phe Thr Phe Arg Ala 
            180                 185                 190         


Val Arg Pro Gln Ala Arg Tyr Cys Ile Gln Val Ala Ala Gln Asp Leu 
        195                 200                 205             


Thr Asp Tyr Gly Glu Ser Ser Asp Trp Ser Leu Pro Ala Ala Pro Ser 
    210                 215                 220                 


Thr Pro Leu Gly Lys Val Pro Gly Val Gly Val Pro Gly Val Gly Phe 
225                 230                 235                 240 


Pro Arg Pro Pro Gly Arg Ser Pro Leu Ser Leu Gln Glu Leu Arg Arg 
                245                 250                 255     


Glu Phe Lys Val Ser Leu Gln Leu Ala Lys Lys Leu Phe Ser Glu Val 
            260                 265                 270         


Arg Ile Gln Ala His His Phe Ala Glu Ser Gln Leu Pro Gly Val Ser 
        275                 280                 285             


Leu Asp Leu Leu Pro Leu Gly Asp Gln Leu Pro Asn Val Ser Leu Pro 
    290                 295                 300                 


Phe Gln Ala Trp His Ser Leu Ser Asp Pro Glu Arg Leu Cys Phe Leu 
305                 310                 315                 320 


Ser Met Met Leu His Pro Phe His Ala Leu Leu Glu Ser Leu Gly Ser 
                325                 330                 335     


Gln Gly Gly Trp Thr Ser Ser Glu Lys Met His Leu Trp Thr Met Arg 
            340                 345                 350         


Leu Asp Leu Arg Asp Leu Gln Arg His Leu Arg Phe Gln Val Glu Tyr 
        355                 360                 365             


Pro Pro Thr Cys Ser Thr Pro Arg Asp Gln Gln Glu Glu Glu Glu Glu 
    370                 375                 380                 


Gln His Glu Glu Arg Lys Gly Leu Leu Ala Ala Ala Pro Gly Gly Pro 
385                 390                 395                 400 


Ser Gln Thr Ala Val Gln Pro Ser Trp Pro Gln Leu Leu Tyr Thr Tyr 
                405                 410                 415     


Gln Leu Leu His Ser Leu Glu Leu Ala Leu Ala Arg Ala Val Arg Asp 
            420                 425                 430         


Leu Leu Leu Leu Ser Gln Ala Gly Asn Pro Ala Pro Pro Val Gly His 
        435                 440                 445             


Ser Thr Phe Gly Ser Gln Pro 
    450                 455 


<210>  8
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  polypeptide targeting EG

<400>  8

Tyr His Trp Tyr Gly Tyr Thr Pro Gln Asn Val Ile 
1               5                   10          


<210>  9
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide targeting GRP78

<400>  9

Ser Asn Thr Arg Val Ala Pro 
1               5           


<210>  10
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide targeting BMPR1b

<400>  10

Ala Ile Ser Met Leu Tyr Leu Asp Glu Asn Glu Lys Val Val Leu 
1               5                   10                  15  


<210>  11
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting petpide 11

<400>  11

Thr Pro Leu Ser Tyr Leu Lys Gly Leu Val Thr Val 
1               5                   10          


<210>  12
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting peptide 12

<400>  12

Asn Pro Tyr His Pro Thr Ile Pro Gln Ser Val His 
1               5                   10          


<210>  13
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting peptide 13

<400>  13

Ala Ser Ala Cys Pro Pro His 
1               5           


<210>  14
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Targeting peptide 14

<400>  14

Gly Gly Pro Asn Leu Thr Gly Arg Trp 
1               5                   


<210>  15
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting petpide 15

<400>  15

Phe Leu Pro Ala Ser Gly Leu 
1               5           


<210>  16
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting petpide 16

<400>  16

Thr Pro Ile Val His His Val Ala 
1               5               


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2p targeting peptide

<400>  17

Thr Val Ala Leu Pro Gly Gly Tyr Val Arg Val 
1               5                   10      


