                         SEQUENCE LISTING

<110>  COLUMBUS EXPLORER, S.L.
 
<120>  Process for the production of closed linear DNA

<130>  P5393PC00

<150>  EP20382064
<151>  2020-01-31

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  293
<212>  PRT
<213>  Thermus thermophilus

<400>  1

Met Arg Pro Ile Glu His Ala Leu Ser Tyr Ala Ala Gln Gly Tyr Gly 
1               5                   10                  15      


Val Leu Pro Leu Arg Pro Gly Gly Lys Glu Pro Leu Gly Lys Leu Val 
            20                  25                  30          


Pro His Gly Leu Lys Asn Ala Ser Arg Asp Pro Ala Thr Leu Glu Ala 
        35                  40                  45              


Trp Trp Arg Ser Cys Pro Arg Cys Gly Val Gly Ile Leu Pro Gly Pro 
    50                  55                  60                  


Glu Val Leu Val Leu Asp Phe Asp Asp Pro Glu Ala Trp Glu Gly Leu 
65                  70                  75                  80  


Arg Gln Glu His Pro Ala Leu Glu Ala Ala Pro Arg Gln Arg Thr Pro 
                85                  90                  95      


Lys Gly Gly Arg His Val Phe Leu Arg Leu Pro Glu Gly Val Arg Leu 
            100                 105                 110         


Ser Ala Ser Val Arg Ala Ile Pro Gly Val Asp Leu Arg Gly Met Gly 
        115                 120                 125             


Arg Ala Tyr Val Val Ala Ala Pro Thr Arg Leu Lys Asp Gly Arg Thr 
    130                 135                 140                 


Tyr Thr Trp Glu Ala Pro Leu Thr Pro Pro Glu Glu Leu Pro Pro Val 
145                 150                 155                 160 


Pro Gln Ala Leu Leu Leu Lys Leu Leu Pro Pro Pro Pro Pro Pro Arg 
                165                 170                 175     


Pro Ser Trp Gly Ala Val Gly Thr Ala Ser Pro Lys Arg Leu Gln Ala 
            180                 185                 190         


Leu Leu Gln Ala Tyr Ala Ala Gln Val Ala Arg Thr Pro Glu Gly Gln 
        195                 200                 205             


Arg His Leu Thr Leu Ile Arg Tyr Ala Val Ala Ala Gly Gly Leu Ile 
    210                 215                 220                 


Pro His Gly Leu Asp Pro Arg Glu Ala Glu Glu Val Leu Val Ala Ala 
225                 230                 235                 240 


Ala Met Ser Ala Gly Leu Pro Glu Trp Glu Ala Arg Asp Ala Val Arg 
                245                 250                 255     


Trp Gly Leu Gly Val Gly Ala Ser Arg Pro Leu Val Leu Glu Ser Ser 
            260                 265                 270         


Ser Lys Pro Pro Glu Pro Arg Thr Tyr Arg Ala Arg Val Tyr Ala Arg 
        275                 280                 285             


Met Arg Arg Trp Val 
    290             


<210>  2
<211>  631
<212>  PRT
<213>  Artificial

<220>
<223>  TelN

<400>  2

Met Ser Lys Val Lys Ile Gly Glu Leu Ile Asn Thr Leu Val Asn Glu 
1               5                   10                  15      


Val Glu Ala Ile Asp Ala Ser Asp Arg Pro Gln Gly Asp Lys Thr Lys 
            20                  25                  30          


Arg Ile Lys Ala Ala Ala Ala Arg Tyr Lys Asn Ala Leu Phe Asn Asp 
        35                  40                  45              


Lys Arg Lys Phe Arg Gly Lys Gly Leu Gln Lys Arg Ile Thr Ala Asn 
    50                  55                  60                  


Thr Phe Asn Ala Tyr Met Ser Arg Ala Arg Lys Arg Phe Asp Asp Lys 
65                  70                  75                  80  


Leu His His Ser Phe Asp Lys Asn Ile Asn Lys Leu Ser Glu Lys Tyr 
                85                  90                  95      


Pro Leu Tyr Ser Glu Glu Leu Ser Ser Trp Leu Ser Met Pro Thr Ala 
            100                 105                 110         


Asn Ile Arg Gln His Met Ser Ser Leu Gln Ser Lys Leu Lys Glu Ile 
        115                 120                 125             


Met Pro Leu Ala Glu Glu Leu Ser Asn Val Arg Ile Gly Ser Lys Gly 
    130                 135                 140                 


Ser Asp Ala Lys Ile Ala Arg Leu Ile Lys Lys Tyr Pro Asp Trp Ser 
145                 150                 155                 160 


Phe Ala Leu Ser Asp Leu Asn Ser Asp Asp Trp Lys Glu Arg Arg Asp 
                165                 170                 175     


Tyr Leu Tyr Lys Leu Phe Gln Gln Gly Ser Ala Leu Leu Glu Glu Leu 
            180                 185                 190         


His Gln Leu Lys Val Asn His Glu Val Leu Tyr His Leu Gln Leu Ser 
        195                 200                 205             


Pro Ala Glu Arg Thr Ser Ile Gln Gln Arg Trp Ala Asp Val Leu Arg 
    210                 215                 220                 


Glu Lys Lys Arg Asn Val Val Val Ile Asp Tyr Pro Thr Tyr Met Gln 
225                 230                 235                 240 


Ser Ile Tyr Asp Ile Leu Asn Asn Pro Ala Thr Leu Phe Ser Leu Asn 
                245                 250                 255     


Thr Arg Ser Gly Met Ala Pro Leu Ala Phe Ala Leu Ala Ala Val Ser 
            260                 265                 270         


Gly Arg Arg Met Ile Glu Ile Met Phe Gln Gly Glu Phe Ala Val Ser 
        275                 280                 285             


Gly Lys Tyr Thr Val Asn Phe Ser Gly Gln Ala Lys Lys Arg Ser Glu 
    290                 295                 300                 


Asp Lys Ser Val Thr Arg Thr Ile Tyr Thr Leu Cys Glu Ala Lys Leu 
305                 310                 315                 320 


Phe Val Glu Leu Leu Thr Glu Leu Arg Ser Cys Ser Ala Ala Ser Asp 
                325                 330                 335     


Phe Asp Glu Val Val Lys Gly Tyr Gly Lys Asp Asp Thr Arg Ser Glu 
            340                 345                 350         


Asn Gly Arg Ile Asn Ala Ile Leu Ala Lys Ala Phe Asn Pro Trp Val 
        355                 360                 365             


Lys Ser Phe Phe Gly Asp Asp Arg Arg Val Tyr Lys Asp Ser Arg Ala 
    370                 375                 380                 


Ile Tyr Ala Arg Ile Ala Tyr Glu Met Phe Phe Arg Val Asp Pro Arg 
385                 390                 395                 400 


Trp Lys Asn Val Asp Glu Asp Val Phe Phe Met Glu Ile Leu Gly His 
                405                 410                 415     


Asp Asp Glu Asn Thr Gln Leu His Tyr Lys Gln Phe Lys Leu Ala Asn 
            420                 425                 430         


Phe Ser Arg Thr Trp Arg Pro Glu Val Gly Asp Glu Asn Thr Arg Leu 
        435                 440                 445             


Val Ala Leu Gln Lys Leu Asp Asp Glu Met Pro Gly Phe Ala Arg Gly 
    450                 455                 460                 


Asp Ala Gly Val Arg Leu His Glu Thr Val Lys Gln Leu Val Glu Gln 
465                 470                 475                 480 


Asp Pro Ser Ala Lys Ile Thr Asn Ser Thr Leu Arg Ala Phe Lys Phe 
                485                 490                 495     


Ser Pro Thr Met Ile Ser Arg Tyr Leu Glu Phe Ala Ala Asp Ala Leu 
            500                 505                 510         


Gly Gln Phe Val Gly Glu Asn Gly Gln Trp Gln Leu Lys Ile Glu Thr 
        515                 520                 525             


Pro Ala Ile Val Leu Pro Asp Glu Glu Ser Val Glu Thr Ile Asp Glu 
    530                 535                 540                 


Pro Asp Asp Glu Ser Gln Asp Asp Glu Leu Asp Glu Asp Glu Ile Glu 
545                 550                 555                 560 


Leu Asp Glu Gly Gly Gly Asp Glu Pro Thr Glu Glu Glu Gly Pro Glu 
                565                 570                 575     


Glu His Gln Pro Thr Ala Leu Lys Pro Val Phe Lys Pro Ala Lys Asn 
            580                 585                 590         


Asn Gly Asp Gly Thr Tyr Lys Ile Glu Phe Glu Tyr Asp Gly Lys His 
        595                 600                 605             


Tyr Ala Trp Ser Gly Pro Ala Asp Ser Pro Met Ala Ala Met Arg Ser 
    610                 615                 620                 


Ala Trp Glu Thr Tyr Tyr Ser 
625                 630     


<210>  3
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  TelN target sequence

<400>  3
tatcagcaca caattgccca ttatacgcgc gtataatgga ctattgtgtg ctgata           56


<210>  4
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  AflII adaptor

<400>  4
ttaagtaaca tttgttggcc actcaggcca acaaatgtta c                           41


<210>  5
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  NheI-HF adaptor

<400>  5
ctagctaaca tttgttggcc actcaggcca acaaatgtta g                           41


<210>  6
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  EcoRV adaptor

<400>  6
tctaacattt gttggccact caggccaaca aatgttagat                             40


<210>  7
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  ScaI adaptor

<400>  7
cttaacattt gttggccact caggccaaca aatgttaagt                             40


<210>  8
<211>  141
<212>  DNA
<213>  Artificial

<220>
<223>  5' ITR sequence

<400>  8
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc t                                                141


<210>  9
<211>  141
<212>  DNA
<213>  Artificial

<220>
<223>  3' ITR sequence

<400>  9
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag ctgcctgcag g                                                141


<210>  10
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 15

<400>  10
agggatccac tcaggat                                                      17


<210>  11
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 37


<220>
<221>  modified_base
<222>  (9)..(13)
<223>  phosphothioated nucleotides

<400>  11
agggatccac tcaggat                                                      17


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 4

<400>  12
agggctaacc actcaggtta g                                                 21


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 28


<220>
<221>  modified_base
<222>  (11)..(11)
<223>  8-oxo-deoxyadenosine

<220>
<221>  modified_base
<222>  (15)..(15)
<223>  8-oxo-deoxyadenosine

<400>  13
agggctaacc actcaggtta g                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 29


<220>
<221>  modified_base
<222>  (12)..(12)
<223>  5-Fluoro-deoxyuracil

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  modified_base
<222>  (14)..(14)
<223>  5-Fluoro-deoxyuracil

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is a, c, g, or t

<400>  14
agggctaacc antnaggtta g                                                 21


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 17

<400>  15
agggataaca tggccactca ggccatgtta t                                      31


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 19


<220>
<221>  modified_base
<222>  (11)..(15)
<223>  LNA nucleotides

<400>  16
agggataaca tggccactca ggcca                                             25


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 22


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  8-oxo-deoxyadenosine

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  8-oxo-deoxyadenosine

<400>  17
agggataaca tggccactca ggccatgtta t                                      31


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 21


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  I

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  I

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  18
agggataaca tggccnctcn ggccatgtta t                                      31


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 41


<220>
<221>  modified_base
<222>  (11)..(14)
<223>  phosphothioated nucleotide

<400>  19
agggcttacg cgcgtaag                                                     18


<210>  20
<211>  3762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP plasmid

<400>  20
ccaatgatgg taccgtatca gcacacaatt gcccattata cgcgcgtata atggactatt       60

gtgtgctgat aggtctcgcc ctgatttaaa tcgtcgaccg ttacataact tacggtaaat      120

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt      180

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa      240

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc      300

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct      360

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag      420

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt      480

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac      540

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc      600

agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc      660

catagaagac accgggaccg atccagcctc cggactctag aggatccggt actcgaggaa      720

ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg      780

gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct ttacttctag      840

gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg cggcccggga      900

tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat      960

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     1020

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     1080

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta     1140

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     1200

ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     1260

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     1320

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     1380

cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg     1440

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     1500

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     1560

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     1620

cgagctgtac aagtaaagcg gccgcgggga tccagacatg ataagataca ttgatgagtt     1680

tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc     1740

tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat     1800

tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttagtcga ccatttaaat     1860

cggtctcgag ggtatcagca cacaattgcc cattatacgc gcgtataatg gactattgtg     1920

tgctgataga agcttatcgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     1980

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     2040

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     2100

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     2160

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     2220

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     2280

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     2340

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     2400

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt     2460

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     2520

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     2580

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     2640

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     2700

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     2760

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     2820

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     2880

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     2940

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     3000

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     3060

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     3120

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     3180

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     3240

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     3300

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     3360

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     3420

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     3480

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     3540

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     3600

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     3660

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     3720

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc                        3762


<210>  21
<211>  4695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Luc plasmid

<400>  21
ccaatgatgg taccgtatca gcacacaatt gcccattata cgcgcgtata atggactatt       60

gtgtgctgat aggtctcgcc ctgatttaaa tcgtcgaccg ttacataact tacggtaaat      120

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt      180

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa      240

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc      300

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct      360

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag      420

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt      480

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac      540

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc      600

agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc      660

catagaagac accgggaccg atccagcctc cggactctag aggatccggt actcgaggaa      720

ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg      780

gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct ttacttctag      840

gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg cggcccggga      900

tccaccggtc gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta      960

tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct     1020

ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga     1080

atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa     1140

tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc     1200

gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct     1260

caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa     1320

aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa     1380

aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg     1440

ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat     1500

aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc     1560

ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac     1620

tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata     1680

tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg     1740

atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt     1800

cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg     1860

gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg     1920

gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg     1980

ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga     2040

tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc     2100

tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga     2160

tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt     2220

tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga     2280

atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga     2340

tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga     2400

aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg     2460

agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat     2520

cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa gcggccgcgg     2580

ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg     2640

aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag     2700

ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga     2760

ggtgtgggag gttttttagt cgaccattta aatcggtctc gagggtatca gcacacaatt     2820

gcccattata cgcgcgtata atggactatt gtgtgctgat agaagcttat cggaaagaac     2880

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     2940

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     3000

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     3060

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     3120

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     3180

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     3240

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     3300

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     3360

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc     3420

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     3480

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     3540

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     3600

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     3660

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     3720

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     3780

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     3840

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     3900

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     3960

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     4020

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     4080

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     4140

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     4200

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     4260

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     4320

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     4380

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     4440

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     4500

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     4560

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     4620

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     4680

gtgccacctg acgtc                                                      4695


<210>  22
<211>  4902
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Luc-ITR plasmid

<400>  22
ccaatgatgg taccgtatca gcacacaatt gcccattata cgcgcgtata atggactatt       60

gtgtgctgat agataggtct cgccctcctg caggcagctg cgcgctcgct cgctcactga      120

ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga      180

gcgagcgcgc agagagggag tggccaactc catcactagg ggttcctgcg gccgcacgcg      240

tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat      300

tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac      360

ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg      420

acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact      480

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt      540

ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc      600

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc      660

gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata      720

taagcagagc tgctagcttg gcattccggt actgttggta aagccaccat ggaagacgcc      780

aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag      840

caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat      900

gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca      960

gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac     1020

tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc     1080

gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc     1140

gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca     1200

atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg     1260

tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag     1320

tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg     1380

cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat     1440

cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat     1500

cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta     1560

atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt     1620

gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac     1680

gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg     1740

gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag     1800

actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa     1860

gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt     1920

aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat     1980

ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct     2040

tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac     2100

aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc     2160

ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt     2220

gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt     2280

caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa     2340

ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag     2400

ggcggaaaga tcgccgtgtc aagccatggc ttcccgccgg cggtggcggc gcaggatgat     2460

ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt     2520

gcttctgcta ggatcaatgt gtaggcggcc aacaatgcga tccgatggcc gcgactctag     2580

agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa     2640

accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct     2700

ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt     2760

atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa     2820

tgtggtaaaa tcgataagcc cgtgcggacc gagcggccgc aggaacccct agtgatggag     2880

ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc     2940

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag     3000

gggtctcgag ggtatcagca cacaattgcc cattatacgc gcgtataatg gactattgtg     3060

tgctgataga agcttatcgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     3120

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     3180

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     3240

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     3300

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     3360

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     3420

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     3480

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     3540

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt     3600

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     3660

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     3720

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     3780

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     3840

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     3900

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     3960

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     4020

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     4080

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     4140

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     4200

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     4260

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     4320

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     4380

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     4440

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     4500

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     4560

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     4620

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     4680

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     4740

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     4800

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     4860

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc                        4902


<210>  23
<211>  4403
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57-Kan_TELO-CMV-EGFP

<400>  23
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgtatcagca cacaattgcc      420

cattatacgc gcgtataatg gactattgtg tgctgataga tttaaatcgt cgaccgttac      480

ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc      540

aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt      600

ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac      660

gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac      720

cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt      780

gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc      840

aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt      900

tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg      960

ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc     1020

acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagagga     1080

tccggtactc gaggaactga aaaaccagaa agttaactgg taagtttagt ctttttgtct     1140

tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg     1200

ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg     1260

tacccgcggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca     1320

ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg     1380

tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca     1440

ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc     1500

agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc     1560

ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc     1620

gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg     1680

acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca     1740

acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc     1800

acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg     1860

gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca     1920

aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga     1980

tcactctcgg catggacgag ctgtacaagt aaagcggccg cggggatcca gacatgataa     2040

gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt     2100

gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta     2160

acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt     2220

agtcgaccat ttaaatctat cagcacacaa ttgcccatta tacgcgcgta taatggacta     2280

ttgtgtgctg atagaagctt ggtgtaatca tggtcatagc tgtttcctgt gtgaaattgt     2340

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt     2400

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg     2460

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     2520

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     2580

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     2640

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     2700

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     2760

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     2820

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     2880

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     2940

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     3000

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     3060

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     3120

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     3180

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     3240

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     3300

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     3360

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     3420

aaatgaagtt ttaaatcaag cccaatctga ataatgttac aaccaattaa ccaattctga     3480

ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat     3540

accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca     3600

taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc     3660

tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac     3720

tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca     3780

gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg     3840

cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga     3900

atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata     3960

ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc     4020

atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt     4080

tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa     4140

caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac     4200

attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg     4260

cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc     4320

agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt     4380

ttgagacacg ggccagagct gca                                             4403


