                               SEQUENCE LISTING

<110> THE GENERAL HOSPITAL CORPORATION
 
<120> METHODS TO CHARACTERIZE ENZYMES FOR GENOME ENGINEERING

<130> 29539-0486WO1

<140> PCT/US2021/014887
<141> 2021-01-25

<150> 62/965,645
<151> 2020-01-24

<160> 14    

<170> PatentIn version 3.5

<210> 1
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
cctcgtgacc tgcgc                                                        15


<210> 2
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (36)..(43)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (46)..(48)
<223> a, c, t, g, unknown or other

<400> 2
gcaggaattc gggaggggca cgggcagctt gccggnnnnn nnnctnnngc gcaggtcacg       60

aggcatg                                                                 67


<210> 3
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (36)..(43)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (46)..(48)
<223> a, c, t, g, unknown or other

<400> 3
gcaggaattc ggagggtcgc cctcgaactt cacctnnnnn nnnctnnngc gcaggtcacg       60

aggcatg                                                                 67


<210> 4
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (13)..(15)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (18)..(27)
<223> a, c, t, g, unknown or other

<400> 4
agaccggaat tcnnngtnnn nnnnnnngga atcccttctg cagcacctgg gcgcaggtca       60

cgaggcatg                                                               69


<210> 5
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (13)..(15)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (18)..(27)
<223> a, c, t, g, unknown or other

<400> 5
agaccggaat tcnnngtnnn nnnnnnnctg atggtccatg tctgttactc gcgcaggtca       60

cgaggcatg                                                               69


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
gggcacgggc agcttgccgg                                                   20


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
gtcgccctcg aacttcacct                                                   20


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
gggcacgggc agcttgccga                                                   20


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
gggcacgggc agcttgccgt                                                   20


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
gggcacgggc agcttgccag                                                   20


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
tggcacgggc agcttgccgg                                                   20


<210> 12
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (40)..(49)
<223> a, c, t, g or absent

<400> 12
gcgactttcc ctcactcctg ctcggtgaat ccgaaaacgn nnnnnnnnn                   49


<210> 13
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (40)..(49)
<223> a, c, t, g or absent

<400> 13
gcgactttcc ctcactcctg ctcggtgaat ccgaaaacgn nnnnnnnnn                   49


<210> 14
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (40)..(49)
<223> a, c, t, g or absent

<400> 14
gcgactttcc ctcactcctg ctcggtgaat ccgaaaacgn nnnnnnnnn                   49


