               SEQUENCE LISTING

<110> Glycom A/S

<120> HMO production

<130> P2939WO00

<150> DK202000087
<151> 2020-01-23

<160> 29

<170> BiSSAP 1.3.6

<210> 1
<211> 392
<212> PRT
<213> Pantoea vagans


<220> 
<223> vag_WP_048785139.1\optimized translation:

<400> 1
Met Lys Ser Leu Leu Thr Arg Lys Arg Arg Ile Asn Pro Val Phe Leu 
1               5                   10                  15      
Ala Phe Met Ala Ala Ser Phe Met Ile Gly Val Ala Gly Ala Leu Gln 
            20                  25                  30          
Ala Pro Thr Leu Ser Leu Phe Leu Thr Arg Glu Val Gln Ala Arg Pro 
        35                  40                  45              
Leu Trp Val Gly Leu Phe Phe Thr Val Asn Ala Ile Ala Gly Ile Val 
    50                  55                  60                  
Val Ser Met Leu Val Ala Lys Arg Ser Asp Ser Arg Gly Asp Arg Arg 
65                  70                  75                  80  
Thr Leu Ile Leu Phe Cys Cys Ala Met Ala Phe Cys Asn Ala Leu Leu 
                85                  90                  95      
Phe Ala Phe Thr Arg His Tyr Leu Thr Leu Ile Thr Leu Gly Val Leu 
            100                 105                 110         
Leu Ser Ala Leu Ala Ser Val Ser Met Pro Gln Ile Phe Ala Leu Ala 
        115                 120                 125             
Arg Glu Tyr Ala Asp Gln Ser Ala Arg Glu Ala Val Met Phe Ser Ser 
    130                 135                 140                 
Val Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 
145                 150                 155                 160 
Ser Phe Ala Leu Ala Leu Asn Phe Gly Phe Val Thr Leu Phe Leu Val 
                165                 170                 175     
Ala Ala Ala Leu Phe Leu Val Cys Ile Leu Leu Ile Lys Phe Thr Leu 
            180                 185                 190         
Pro Ser Val Pro Arg Ala Glu Pro Leu Met Arg Ser Gly Gly Met Pro 
        195                 200                 205             
Leu Ser Gly Trp Arg Asp Arg Asp Val Arg Leu Leu Phe Ile Ala Ser 
    210                 215                 220                 
Val Thr Met Trp Thr Cys Asn Thr Met Tyr Ile Ile Asp Met Pro Leu 
225                 230                 235                 240 
Tyr Ile Ser Val Thr Leu Gly Leu Pro Glu Lys Leu Ala Gly Leu Leu 
                245                 250                 255     
Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Val Met Leu Leu Ala Gly 
            260                 265                 270         
His Tyr Ala Lys Arg Val Gly Lys Arg Asn Leu Met Leu Ile Ala Val 
        275                 280                 285             
Ala Ala Gly Val Leu Phe Tyr Ala Gly Leu Ala Met Phe Ala Ser Gln 
    290                 295                 300                 
Thr Ala Leu Met Ala Leu Gln Leu Phe Asn Ala Val Phe Ile Gly Ile 
305                 310                 315                 320 
Ile Ala Gly Ile Gly Met Leu Trp Phe Gln Asp Leu Met Pro Gly Arg 
                325                 330                 335     
Pro Gly Ala Ala Thr Thr Met Phe Thr Asn Ser Ile Ser Thr Gly Met 
            340                 345                 350         
Ile Leu Ala Gly Val Ile Gln Gly Thr Leu Ser Glu Arg Phe Gly His 
        355                 360                 365             
Ile Ala Val Tyr Trp Leu Ala Leu Gly Leu Ala Val Ala Ala Phe Ala 
    370                 375                 380                 
Met Ser Ala Arg Val Lys Asn Val 
385                 390         

<210> 2
<211> 1182
<212> DNA
<213> Pantoea vagans


<220> 
<223> vag nucleotide sequence

<400> 2
atgaagagcg cgctgacctt cagccgtcgt attaacccgg tttttctggc gttctttgtg      60

gttgcgttcc tgagcggtat tgcgggtgcg ctgcaggcgc cgaccctgag cctgttcctg     120

agcaccgagg tgaaagttcg tccgctgtgg gtgggcctgt tctacaccgt taacgcgatt     180

gcgggtatca ccgtgagctt tgttctggcg aagcgtagcg acctgcgtgg cgatcgtcgt     240

aaactgatcc tggtgtgcta cctgatggcg gttggtaact gcctgctgtt cgcgtttaac     300

cgtgactatc tgaccctgat taccgcgggc gtgctgctgg cggcggttgc gaacaccgcg     360

atgccgcaga ttttcgcgct ggcgcgtgag tacgcggata acagcgcgcg tgaagtggtt     420

atgtttagca gcattatgcg tgcgcaactg agcctggcgt gggttatcgg tccgccgctg     480

agcttcatgc tggcgctgaa ctatggcttc accctgatgt tttgcattgc ggcgggtatc     540

ttcgtgctga gcgcgctggt tgtgtggttt attctgccga gcgtgcagcg tgcggaaccg     600

gttatggatg cgccgaccgt ggcgcaaggc agcctgttcg cggacaagga tgttctgctg     660

ctgtttattg cgagcatgct gatgtggacc tgcaacacca tgtacatcat tgatatgccg     720

ctgtatatca ccgcgagcct gggtctgccg gagcgtctgg cgggtctgct gatgggcacc     780

gcggcgggtc tggaaatccc gattatgctg ctggcgggct acagcgtgcg tcgttttggc     840

aagcgtaaaa tcatgctgtt cgcggtgctg gcgggcgttc tgttttatac cggtctggtt     900

ctgttcaagt ttaaaagcgc gctgatgctg ctgcagattt tcaacgcgat ctttattggt     960

atcgtggcgg gtatcggcat gctgtacttc caagacctga tgccgggtcg tgcgggtgcg    1020

gcgaccaccc tgtttaccaa cagcattagc accggcgtta tcctggcggg cgtgctgcaa    1080

ggtgttctga ccgaaacctg gggtcacaac agcgtgtatg ttatggcgat gattctggcg    1140

atcctgagcc tgatcatttg cgcgcgtgtg cgtgaagcgt aa                       1182


<210> 3
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> PglpF regulatory sequence

<300> 
<310> WO2019123324
<311> 19.12.2018
<312> 27.06.2019
<313> 1-300

<400> 3
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 4
<211> 195
<212> DNA
<213> Artificial Sequence


<220> 
<223> Plac expression element

<300> 
<310> WO2019123324
<311> 19.12.2018
<312> 27.06.2019

<400> 4
atgcgcaaat tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc     60

cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg    120

accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtacct    180

aaggaggaaa cagct                                                     195


<210> 5
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O40 backbone.for

<220> 
<221> misc_RNA
<222> 9

<400> 5
attaacccuc caggcatcaa ataaaacgaa aggc                                 34


<210> 6
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O68, Plac.for

<400> 6
atgcgcaaau tgtgagttag ctcactcatt ag                                   32


<210> 7
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O79 Backbone.rev

<220> 
<221> misc_RNA
<222> 10

<400> 7
atttgcgcau caccaatcaa attcacgcgg cc                                   32


<210> 8
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O113, Plac.rev

<400> 8
agctgttucc tccttaggta cccagctttt gttccc                               36


<210> 9
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O261, PglpF.for

<220> 
<221> misc_RNA
<222> 10

<400> 9
atgcgcaaau gcggcacgcc ttgcagatta cg                                   32


<210> 10
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O262, PglpF.rev

<220> 
<221> misc_RNA
<222> 8

<400> 10
agctgttucc tccttggtta atgtttgttg tatgcg                               36


<210> 11
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY745, vag.for

<400> 11
aaacagcuat gaagagcctg ctgacccgta aac                                  33


<210> 12
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY746, vag.rev

<220> 
<221> misc_RNA
<222> 9

<400> 12
agggttaaut taaacgtttt tcacacgcgc g                                   31


<210> 13
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY721, yberC0001_9420.for

<220> 
<221> misc_RNA
<222> 8

<400> 13
aaacagcuat gaagagcgcg ctgaccttta gc                                   32


<210> 14
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY722, yberC0001_9420.rev

<220> 
<221> misc_RNA
<222> 9

<400> 14
agggttaaut tacgcctcac gcacacgcg                                      29


<210> 15
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer KABY733, fred.for

<220> 
<221> misc_RNA
<222> 8

<400> 15
aaacagcuat gaagagcgcg ctgaccttca g                                   31


<210> 16
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY734, fred.rev

<220> 
<221> misc_RNA
<222> 9

<400> 16
agggttaaut tacgcttcac gcacacgcg                                      29


<210> 17
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY729, bad.for

<220> 
<221> misc_RNA
<222> 8

<400> 17
aaacagcuat gagcagccgt cgtctgagc                                      29


<210> 18
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY730, bad.rev

<220> 
<221> misc_RNA
<222> 9

<400> 18
agggttaaut tacacgtttt taacacgggt catcag                               36


<210> 19
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY741, nec.for

<220> 
<221> misc_RNA
<222> 8

<400> 19
aaacagcuat gcagagcttc accccgcc                                       28


<210> 20
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY742, nec.rev

<220> 
<221> misc_RNA
<222> 9

<400> 20
agggttaaut tacgcctgct ctttaacacg cagc                                 34


<210> 21
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY737, marc.for

<220> 
<221> misc_RNA
<222> 8

<400> 21
aaacagcuat gcagcgtctg agccgtctga g                                   31


<210> 22
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer KABY738, marc.rev

<220> 
<221> misc_RNA
<222> 9

<400> 22
agggttaaut taaacttcac gcactttcgc gc                                   32


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O48, galK.for

<400> 23
cccagcgaga cctgaccgca gaac                                           24


<210> 24
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer O49, glaK.rev

<400> 24
ccccagtcca tcagcgtgac tacc                                           24


<210> 25
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer MP1217, yabM.for

<220> 
<221> misc_RNA
<222> 8

<400> 25
aaacagcuat gaaggcgctg tggagccgtc g                                   31


<210> 26
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer MP1218, yabM.rev

<220> 
<221> misc_RNA
<222> 9

<400> 26
agggttaauc gccagcggaa cgctcttcac g                                   31


<210> 27
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter sequence PmglB_70UTR

<400> 27
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa ggaggaaaca gct                                            203


<210> 28
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter sequence PmglB_70UTR_SD4

<400> 28
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa ctaggaaaca gct                                            203


<210> 29
<211> 291
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter sequence PgatY_70UTR

<400> 29
cggcaaccta tgcctgatgc gacgctgaag cgtcttatca tgcctacata gcactgccac     60

gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg    120

gaggccgttt ttttgccttt cactcctcga ataattttca tattgtcgtt tttgtgatcg    180

ttatctcgat atttaaaaac aaataatttc attatatttt gtgcctacaa gcatcgtgga    240

ggtccgtgac tttcacgcat acaacaaaca ttaaccaagg aggaaacagc t             291


