               SEQUENCE LISTING

						RECTIFIED UNDER RULE 91 ISA/EP

<110> Glycom A/S



<120> HMO production



<130> P2940WO00



<150> DK202000088

<151> 2020-01-23



<160> 17



<170> BiSSAP 1.3.6



<210> 1

<211> 393

<212> PRT

<213> Yersinia bercovieri





<220> 

<223> YberC_EEQ08298.1 \optimized translation



<400> 1

Met Lys Ser Ala Leu Thr Phe Ser Arg Arg Ile Asn Pro Val Phe Leu 

1               5                   10                  15      

Ala Phe Phe Val Val Ala Phe Leu Ser Gly Ile Ala Gly Ala Leu Gln 

            20                  25                  30          

Ala Pro Thr Leu Ser Leu Phe Leu Ser Thr Glu Val Lys Val Arg Pro 

        35                  40                  45              

Leu Trp Val Gly Leu Phe Tyr Thr Val Asn Ala Ile Ala Gly Ile Thr 

    50                  55                  60                  

Val Ser Phe Ile Leu Ala Lys Arg Ser Asp Ser Arg Gly Asp Arg Arg 

65                  70                  75                  80  

Lys Leu Ile Met Val Cys Tyr Leu Met Ala Val Gly Asn Cys Leu Leu 

                85                  90                  95      

Phe Ala Phe Asn Arg Asp Tyr Leu Thr Leu Ile Thr Ala Gly Val Leu 

            100                 105                 110         

Leu Ala Ser Val Ala Asn Thr Ala Met Pro Gln Ile Phe Ala Leu Ala 

        115                 120                 125             

Arg Glu Tyr Ala Asp Ser Ser Ala Arg Glu Val Val Met Phe Ser Ser 

    130                 135                 140                 

Ile Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 

145                 150                 155                 160 

Ser Phe Met Leu Ala Leu Asn Tyr Gly Phe Thr Leu Met Phe Ser Ile 

                165                 170                 175     

Ala Ala Gly Ile Phe Val Leu Ser Ala Leu Val Val Trp Phe Ile Leu 

            180                 185                 190         

Pro Ser Val Pro Arg Ala Glu Pro Val Val Asp Ala Pro Val Val Val 

        195                 200                 205             

Gln Gly Ser Leu Phe Ala Asp Lys Asn Val Leu Leu Leu Phe Ile Ala 

    210                 215                 220                 

Ser Met Leu Met Trp Thr Cys Asn Thr Met Tyr Ile Ile Asp Met Pro 

225                 230                 235                 240 

Leu Tyr Ile Thr Ala Ser Leu Gly Leu Pro Glu Arg Leu Ala Gly Leu 

                245                 250                 255     

Leu Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Ile Met Leu Leu Ala 

            260                 265                 270         

Gly Tyr Ser Val Arg Tyr Phe Gly Lys Arg Lys Ile Met Leu Phe Ala 

        275                 280                 285             

Val Leu Ala Gly Val Leu Phe Tyr Thr Gly Leu Val Leu Phe Lys Phe 

    290                 295                 300                 

Lys Thr Ala Leu Met Leu Leu Gln Ile Phe Asn Ala Ile Phe Ile Gly 

305                 310                 315                 320 

Ile Val Ala Gly Ile Gly Met Leu Tyr Phe Gln Asp Leu Met Pro Gly 

                325                 330                 335     

Arg Ala Gly Ala Ala Thr Thr Leu Phe Thr Asn Ser Ile Ser Thr Gly 

            340                 345                 350         

Val Ile Leu Ala Gly Val Leu Gln Gly Gly Leu Thr Glu Thr Trp Gly 

        355                 360                 365             

His Asp Ser Val Tyr Val Met Ala Met Val Leu Ser Ile Leu Ala Leu 

    370                 375                 380                 

Ile Ile Cys Ala Arg Val Arg Glu Ala 

385                 390             



<210> 2

<211> 1182

<212> DNA

<213> Yersinia bercovieri





<220> 

<223> YberC nucleotide sequence



<400> 2

atgaagagcg cgctgacctt tagccgtcgt atcaatccgg tgtttctggc gtttttcgtg      60



gtggcgttcc tgagcggtat tgcgggtgcg ctgcaggcgc cgaccctgag cctgtttctg     120



agcaccgagg ttaaagtgcg tccgctgtgg gttggcctgt tttacaccgt gaacgcgatt     180



gcgggtatca ccgttagctt cattctggcg aagcgtagcg acagccgtgg cgatcgtcgt     240



aaactgatca tggtttgcta cctgatggcg gtgggtaact gcctgctgtt cgcgtttaac     300



cgtgactatc tgaccctgat taccgcgggc gttctgctgg cgagcgtggc gaacaccgcg     360



atgccgcaga ttttcgcgct ggcgcgtgag tatgcggata gcagcgcgcg tgaagtggtt     420



atgttcagca gcattatgcg tgcgcaactg agcctggcgt gggtgattgg tccgccgctg     480



agcttcatgc tggcgctgaa ctatggcttc accctgatgt ttagcattgc ggcgggtatc     540



tttgttctga gcgcgctggt tgtgtggttc attctgccga gcgtgccgcg tgcggaaccg     600



gtggttgacg cgccggtggt tgtgcagggc agcctgtttg cggataaaaa cgttctgctg     660



ctgttcattg cgagcatgct gatgtggacc tgcaacacca tgtacatcat tgacatgccg     720



ctgtatatca ccgcgagcct gggtctgccg gagcgtctgg cgggtctgct gatgggcacc     780



gcggcgggtc tggaaatccc gattatgctg ctggcgggct acagcgtgcg ttatttcggc     840



aagcgtaaaa tcatgctgtt tgcggttctg gcgggcgtgc tgttctacac cggtctggtt     900



ctgttcaagt ttaaaaccgc gctgatgctg ctgcagattt tcaacgcgat ctttattggc     960



atcgtggcgg gtatcggcat gctgtatttt caagacctga tgccgggtcg tgcgggtgcg    1020



gcgaccaccc tgttcaccaa cagcattagc accggcgtta tcctggcggg tgtgctgcaa    1080



ggtggcctga ccgaaacctg gggtcacgat agcgtttatg tgatggcgat ggttctgagc    1140



attctggcgc tgattatctg cgcgcgtgtg cgtgaggcgt aa                       1182





<210> 3

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF expression element



<300> 

<310> WO2019123324

<311> 19.12.2018

<312> 27.06.2019



<400> 3

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300





<210> 4

<211> 195

<212> DNA

<213> Artificial Sequence





<220> 

<223> Plac expression element



<300> 

<310> WO2019123324

<311> 19.12.2018

<312> 27.06.2019



<400> 4

atgcgcaaat tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc     60



cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg    120



accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtacct    180



aaggaggaaa cagct                                                     195





<210> 5

<211> 34

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O40 backbone.for



<220> 

<221> misc_RNA

<222> 9



<400> 5

attaacccuc caggcatcaa ataaaacgaa aggc                                 34





<210> 6

<211> 32

<212> DNA

<213> Artificial Sequence





<220> 

<223> Plac.for



<400> 6

atgcgcaaau tgtgagttag ctcactcatt ag                                   32





<210> 7

<211> 32

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O79 Backbone.rev



<220> 

<221> misc_RNA

<222> 10



<400> 7

atttgcgcau caccaatcaa attcacgcgg cc                                   32





<210> 8

<211> 36

<212> DNA

<213> Artificial Sequence





<220> 

<223> Plac.rev



<400> 8

agctgttucc tccttaggta cccagctttt gttccc                               36





<210> 9

<211> 32

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O261, PglpF.for



<220> 

<221> misc_RNA

<222> 10



<400> 9

atgcgcaaau gcggcacgcc ttgcagatta cg                                   32





<210> 10

<211> 36

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O262, PglpF.rev



<220> 

<221> misc_RNA

<222> 8



<400> 10

agctgttucc tccttggtta atgtttgttg tatgcg                               36





<210> 11

<211> 32

<212> DNA

<213> Artificial Sequence





<220> 

<223> primer KABY721, yberC0001_9420.for



<220> 

<221> misc_RNA

<222> 8



<400> 11

aaacagcuat gaagagcgcg ctgaccttta gc                                   32





<210> 12

<211> 29

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer KABY722, yberC0001_9420.rev



<220> 

<221> misc_RNA

<222> 9



<400> 12

agggttaaut tacgcctcac gcacacgcg                                      29





<210> 13

<211> 24

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O48, galK.for



<400> 13

cccagcgaga cctgaccgca gaac                                           24





<210> 14

<211> 24

<212> DNA

<213> Artificial Sequence





<220> 

<223> Primer O49, glaK.rev



<400> 14

ccccagtcca tcagcgtgac tacc                                           24





<210> 15

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> Promoter sequence PmglB_70UTR



<400> 15

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ggaggaaaca gct                                            203





<210> 16

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> Promoter sequence PmglB_70UTR_SD4



<400> 16

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ctaggaaaca gct                                            203





<210> 17

<211> 291

<212> DNA

<213> Artificial Sequence





<220> 

<223> Promoter sequence PgatY_70UTR



<400> 17

cggcaaccta tgcctgatgc gacgctgaag cgtcttatca tgcctacata gcactgccac     60



gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg    120



gaggccgttt ttttgccttt cactcctcga ataattttca tattgtcgtt tttgtgatcg    180



ttatctcgat atttaaaaac aaataatttc attatatttt gtgcctacaa gcatcgtgga    240



ggtccgtgac tttcacgcat acaacaaaca ttaaccaagg aggaaacagc t             291





