                         SEQUENCE LISTING

<110>  The General Hospital Corporation
       Whitehead Institute for Biomedical Research
 
<120>  The Kinase NEK10 and Its Use in Treating and Diagnosing 
       Bronchiectasis and Other Respiratory Disorders

<130>  29539-0495WO1

<150>  US 62/962,434
<151>  2020-01-17

<150>  US 62/961,817
<151>  2020-01-16

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  1172
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Asp Gln Asp Lys Lys Val Lys Thr Thr Glu Lys Ser Thr Asp 
1               5                   10                  15      


Lys Gln Gln Glu Ile Thr Ile Arg Asp Tyr Ser Asp Leu Lys Arg Leu 
            20                  25                  30          


Arg Cys Leu Leu Asn Val Gln Ser Ser Lys Gln Gln Leu Pro Ala Ile 
        35                  40                  45              


Asn Phe Asp Ser Ala Gln Asn Ser Met Thr Lys Ser Glu Pro Ala Ile 
    50                  55                  60                  


Arg Ala Gly Gly His Arg Ala Arg Gly Gln Trp His Glu Ser Thr Glu 
65                  70                  75                  80  


Ala Val Glu Leu Glu Asn Phe Ser Ile Asn Tyr Lys Asn Glu Arg Asn 
                85                  90                  95      


Phe Ser Lys His Pro Gln Arg Lys Leu Phe Gln Glu Ile Phe Thr Ala 
            100                 105                 110         


Leu Val Lys Asn Arg Leu Ile Ser Arg Glu Trp Val Asn Arg Ala Pro 
        115                 120                 125             


Ser Ile His Phe Leu Arg Val Leu Ile Cys Leu Arg Leu Leu Met Arg 
    130                 135                 140                 


Asp Pro Cys Tyr Gln Glu Ile Leu His Ser Leu Gly Gly Ile Glu Asn 
145                 150                 155                 160 


Leu Ala Gln Tyr Met Glu Ile Val Ala Asn Glu Tyr Leu Gly Tyr Gly 
                165                 170                 175     


Glu Glu Gln His Thr Val Asp Lys Leu Val Asn Met Thr Tyr Ile Phe 
            180                 185                 190         


Gln Lys Leu Ala Ala Val Lys Asp Gln Arg Glu Trp Val Thr Thr Ser 
        195                 200                 205             


Gly Ala His Lys Thr Leu Val Asn Leu Leu Gly Ala Arg Asp Thr Asn 
    210                 215                 220                 


Val Leu Leu Gly Ser Leu Leu Ala Leu Ala Ser Leu Ala Glu Ser Gln 
225                 230                 235                 240 


Glu Cys Arg Glu Lys Ile Ser Glu Leu Asn Ile Val Glu Asn Leu Leu 
                245                 250                 255     


Met Ile Leu His Glu Tyr Asp Leu Leu Ser Lys Arg Leu Thr Ala Glu 
            260                 265                 270         


Leu Leu Arg Leu Leu Cys Ala Glu Pro Gln Val Lys Glu Gln Val Lys 
        275                 280                 285             


Leu Tyr Glu Gly Ile Pro Val Leu Leu Ser Leu Leu His Ser Asp His 
    290                 295                 300                 


Leu Lys Leu Leu Trp Ser Ile Val Trp Ile Leu Val Gln Val Cys Glu 
305                 310                 315                 320 


Asp Pro Glu Thr Ser Val Glu Ile Arg Ile Trp Gly Gly Ile Lys Gln 
                325                 330                 335     


Leu Leu His Ile Leu Gln Gly Asp Arg Asn Phe Val Ser Asp His Ser 
            340                 345                 350         


Ser Ile Gly Ser Leu Ser Ser Ala Asn Ala Ala Gly Arg Ile Gln Gln 
        355                 360                 365             


Leu His Leu Ser Glu Asp Leu Ser Pro Arg Glu Ile Gln Glu Asn Thr 
    370                 375                 380                 


Phe Ser Leu Gln Ala Ala Cys Cys Ala Ala Leu Thr Glu Leu Val Leu 
385                 390                 395                 400 


Asn Asp Thr Asn Ala His Gln Val Val Gln Glu Asn Gly Val Tyr Thr 
                405                 410                 415     


Ile Ala Lys Leu Ile Leu Pro Asn Lys Gln Lys Asn Ala Ala Lys Ser 
            420                 425                 430         


Asn Leu Leu Gln Cys Tyr Ala Phe Arg Ala Leu Arg Phe Leu Phe Ser 
        435                 440                 445             


Met Glu Arg Asn Arg Pro Leu Phe Lys Arg Leu Phe Pro Thr Asp Leu 
    450                 455                 460                 


Phe Glu Ile Phe Ile Asp Ile Gly His Tyr Val Arg Asp Ile Ser Ala 
465                 470                 475                 480 


Tyr Glu Glu Leu Val Ser Lys Leu Asn Leu Leu Val Glu Asp Glu Leu 
                485                 490                 495     


Lys Gln Ile Ala Glu Asn Ile Glu Ser Ile Asn Gln Asn Lys Ala Pro 
            500                 505                 510         


Leu Lys Tyr Ile Gly Asn Tyr Ala Ile Leu Asp His Leu Gly Ser Gly 
        515                 520                 525             


Ala Phe Gly Cys Val Tyr Lys Val Arg Lys His Ser Gly Gln Asn Leu 
    530                 535                 540                 


Leu Ala Met Lys Glu Val Asn Leu His Asn Pro Ala Phe Gly Lys Asp 
545                 550                 555                 560 


Lys Lys Asp Arg Asp Ser Ser Val Arg Asn Ile Val Ser Glu Leu Thr 
                565                 570                 575     


Ile Ile Lys Glu Gln Leu Tyr His Pro Asn Ile Val Arg Tyr Tyr Lys 
            580                 585                 590         


Thr Phe Leu Glu Asn Asp Arg Leu Tyr Ile Val Met Glu Leu Ile Glu 
        595                 600                 605             


Gly Ala Pro Leu Gly Glu His Phe Ser Ser Leu Lys Glu Lys His His 
    610                 615                 620                 


His Phe Thr Glu Glu Arg Leu Trp Lys Ile Phe Ile Gln Leu Cys Leu 
625                 630                 635                 640 


Ala Leu Arg Tyr Leu His Lys Glu Lys Arg Ile Val His Arg Asp Leu 
                645                 650                 655     


Thr Pro Asn Asn Ile Met Leu Gly Asp Lys Asp Lys Val Thr Val Thr 
            660                 665                 670         


Asp Phe Gly Leu Ala Lys Gln Lys Gln Glu Asn Ser Lys Leu Thr Ser 
        675                 680                 685             


Val Val Gly Thr Ile Leu Tyr Ser Cys Pro Glu Val Leu Lys Ser Glu 
    690                 695                 700                 


Pro Tyr Gly Glu Lys Ala Asp Val Trp Ala Val Gly Cys Ile Leu Tyr 
705                 710                 715                 720 


Gln Met Ala Thr Leu Ser Pro Pro Phe Tyr Ser Thr Asn Met Leu Ser 
                725                 730                 735     


Leu Ala Thr Lys Ile Val Glu Ala Val Tyr Glu Pro Val Pro Glu Gly 
            740                 745                 750         


Ile Tyr Ser Glu Lys Val Thr Asp Thr Ile Ser Arg Cys Leu Thr Pro 
        755                 760                 765             


Asp Ala Glu Ala Arg Pro Asp Ile Val Glu Val Ser Ser Met Ile Ser 
    770                 775                 780                 


Asp Val Met Met Lys Tyr Leu Asp Asn Leu Ser Thr Ser Gln Leu Ser 
785                 790                 795                 800 


Leu Glu Lys Lys Leu Glu Arg Glu Arg Arg Arg Thr Gln Arg Tyr Phe 
                805                 810                 815     


Met Glu Ala Asn Arg Asn Thr Val Thr Cys His His Glu Leu Ala Val 
            820                 825                 830         


Leu Ser His Glu Thr Phe Glu Lys Ala Ser Leu Ser Ser Ser Ser Ser 
        835                 840                 845             


Gly Ala Ala Ser Leu Lys Ser Glu Leu Ser Glu Ser Ala Asp Leu Pro 
    850                 855                 860                 


Pro Glu Gly Phe Gln Ala Ser Tyr Gly Lys Asp Glu Asp Arg Ala Cys 
865                 870                 875                 880 


Asp Glu Ile Leu Ser Asp Asp Asn Phe Asn Leu Glu Asn Ala Glu Lys 
                885                 890                 895     


Asp Thr Tyr Ser Glu Val Asp Asp Glu Leu Asp Ile Ser Asp Asn Ser 
            900                 905                 910         


Ser Ser Ser Ser Ser Ser Pro Leu Lys Glu Ser Thr Phe Asn Ile Leu 
        915                 920                 925             


Lys Arg Ser Phe Ser Ala Ser Gly Gly Glu Arg Gln Ser Gln Thr Arg 
    930                 935                 940                 


Asp Phe Thr Gly Gly Thr Gly Ser Arg Pro Arg Pro Ala Leu Leu Pro 
945                 950                 955                 960 


Leu Asp Leu Leu Leu Lys Val Pro Pro His Met Leu Arg Ala His Ile 
                965                 970                 975     


Lys Glu Ile Glu Ala Glu Leu Val Thr Gly Trp Gln Ser His Ser Leu 
            980                 985                 990         


Pro Ala Val Ile Leu Arg Asn Leu  Lys Asp His Gly Pro  Gln Met Gly 
        995                 1000                 1005             


Thr Phe  Leu Trp Gln Ala Ser  Ala Gly Ile Ala Val  Ser Gln Arg 
    1010                 1015                 1020             


Lys Val  Arg Gln Ile Ser Asp  Pro Ile Gln Gln Ile  Leu Ile Gln 
    1025                 1030                 1035             


Leu His  Lys Ile Ile Tyr Ile  Thr Gln Leu Pro Pro  Ala Leu His 
    1040                 1045                 1050             


His Asn  Leu Lys Arg Arg Val  Ile Glu Arg Phe Lys  Lys Ser Leu 
    1055                 1060                 1065             


Phe Ser  Gln Gln Ser Asn Pro  Cys Asn Leu Lys Ser  Glu Ile Lys 
    1070                 1075                 1080             


Lys Leu  Ser Gln Gly Ser Pro  Glu Pro Ile Glu Pro  Asn Phe Phe 
    1085                 1090                 1095             


Thr Ala  Asp Tyr His Leu Leu  His Arg Ser Ser Gly  Gly Asn Ser 
    1100                 1105                 1110             


Leu Ser  Pro Asn Asp Pro Thr  Gly Leu Pro Thr Ser  Ile Glu Leu 
    1115                 1120                 1125             


Glu Glu  Gly Ile Thr Tyr Glu  Gln Met Gln Thr Val  Ile Glu Glu 
    1130                 1135                 1140             


Val Leu  Glu Glu Ser Gly Tyr  Tyr Asn Phe Thr Ser  Asn Arg Tyr 
    1145                 1150                 1155             


His Ser  Tyr Pro Trp Gly Thr  Lys Asn His Pro Thr  Lys Arg 
    1160                 1165                 1170         


<210>  2
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  2
agcctgtcca gtgcaaatg                                                    19


<210>  3
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  3
ggcattggtg tcattgagc                                                    19


<210>  4
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  4
gcatgaatcc acagaagctg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  5
tacgctgagg atgtttgctg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  6
gagcccaact ttttcacagc                                                   20


<210>  7
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  7
ccaattcaat gctggttgg                                                    19


<210>  8
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  8
tcagtggaga aggagttgga c                                                 21


<210>  9
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  9
ctgccatatc cagaggaaac ac                                                22


<210>  10
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  10
ccaaaagccc agagaaagc                                                    19


<210>  11
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  11
agttggggat cttcagcttc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  12
tggatcacgg acaacttctg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  13
gaggcacttt gatgaagcac                                                   20


<210>  14
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  14
gctctttgag ttgatgcctg tc                                                22


<210>  15
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  15
cagcggcaat gtagttcaag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  16
agacaaatcg ctccaccaac                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  17
cctgcggctt aatttgactc                                                   20


<210>  18
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  18
tctggggcat tgatgtttta ca                                                22


<210>  19
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  19
ccccatggca tcttggtctt                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  20
gttccccaga acgaacggat                                                   20


<210>  21
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  21
catgtctgcg gggactctc                                                    19


<210>  22
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  22
gcccggcaca aaattaagca                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  23
gatgcagagg aagcagcagt                                                   20


<210>  24
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  24
caccggtctg agcccgccat caggg                                             25


<210>  25
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  25
aaacccctga tggcgggctc agacc                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  26
caccggcggg tggacacaga gctcg                                             25


<210>  27
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  27
aaaccgagct ctgtgtccac ccgcc                                             25


<210>  28
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  28
caccggctgc tcttctccat agccg                                             25


<210>  29
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  29
aaaccggcta tggagaagag cagcc                                             25


<210>  30
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  30
ttccagccca agtgcaaaga                                                   20


<210>  31
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  31
tgatgaatgt tccttaaaac aagca                                             25


