                               SEQUENCE LISTING

<110> SYNTHEKINE, INC.
 
<120> IL2 MUTEINS

<130> 1218661

<140> PCT/US2021/013514
<141> 2021-01-14

<150> 62/960,847
<151> 2020-01-14

<160> 101   

<170> PatentIn version 3.5

<210> 1
<211> 251
<212> PRT
<213> Homo sapiens

<400> 1
Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys 
1               5                   10                  15      


Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg 
            20                  25                  30          


Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly 
        35                  40                  45              


Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser 
    50                  55                  60                  


Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln 
65                  70                  75                  80  


Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp 
                85                  90                  95      


Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn 
            100                 105                 110         


Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr 
        115                 120                 125             


Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu 
    130                 135                 140                 


Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln 
145                 150                 155                 160 


Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu 
                165                 170                 175     


Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys 
            180                 185                 190         


Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr 
        195                 200                 205             


Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln Val Ala Val Ala Gly 
    210                 215                 220                 


Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu Ser Gly Leu Thr Trp 
225                 230                 235                 240 


Gln Arg Arg Gln Arg Lys Ser Arg Arg Thr Ile 
                245                 250     


<210> 2
<211> 525
<212> PRT
<213> Homo sapiens

<400> 2
Ala Val Asn Gly Thr Ser Gln Phe Thr Cys Phe Tyr Asn Ser Arg Ala 
1               5                   10                  15      


Asn Ile Ser Cys Val Trp Ser Gln Asp Gly Ala Leu Gln Asp Thr Ser 
            20                  25                  30          


Cys Gln Val His Ala Trp Pro Asp Arg Arg Arg Trp Asn Gln Thr Cys 
        35                  40                  45              


Glu Leu Leu Pro Val Ser Gln Ala Ser Trp Ala Cys Asn Leu Ile Leu 
    50                  55                  60                  


Gly Ala Pro Asp Ser Gln Lys Leu Thr Thr Val Asp Ile Val Thr Leu 
65                  70                  75                  80  


Arg Val Leu Cys Arg Glu Gly Val Arg Trp Arg Val Met Ala Ile Gln 
                85                  90                  95      


Asp Phe Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro Ile Ser Leu 
            100                 105                 110         


Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser Trp Glu Ile 
        115                 120                 125             


Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe Glu Ala Arg 
    130                 135                 140                 


Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu Leu Thr Leu 
145                 150                 155                 160 


Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr Pro Asp Thr 
                165                 170                 175     


Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly Glu Phe Thr 
            180                 185                 190         


Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr Lys Pro Ala 
        195                 200                 205             


Ala Leu Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly 
    210                 215                 220                 


Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn 
225                 230                 235                 240 


Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr 
                245                 250                 255     


Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly 
            260                 265                 270         


Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser 
        275                 280                 285             


Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg 
    290                 295                 300                 


Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro 
305                 310                 315                 320 


Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln 
                325                 330                 335     


Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys 
            340                 345                 350         


Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu 
        355                 360                 365             


Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro 
    370                 375                 380                 


Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp 
385                 390                 395                 400 


Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser 
                405                 410                 415     


Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser 
            420                 425                 430         


Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro 
        435                 440                 445             


Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu 
    450                 455                 460                 


Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg 
465                 470                 475                 480 


Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe 
                485                 490                 495     


Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser 
            500                 505                 510         


Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        515                 520                 525 


<210> 3
<211> 214
<212> PRT
<213> Homo sapiens

<400> 3
Ala Val Asn Gly Thr Ser Gln Phe Thr Cys Phe Tyr Asn Ser Arg Ala 
1               5                   10                  15      


Asn Ile Ser Cys Val Trp Ser Gln Asp Gly Ala Leu Gln Asp Thr Ser 
            20                  25                  30          


Cys Gln Val His Ala Trp Pro Asp Arg Arg Arg Trp Asn Gln Thr Cys 
        35                  40                  45              


Glu Leu Leu Pro Val Ser Gln Ala Ser Trp Ala Cys Asn Leu Ile Leu 
    50                  55                  60                  


Gly Ala Pro Asp Ser Gln Lys Leu Thr Thr Val Asp Ile Val Thr Leu 
65                  70                  75                  80  


Arg Val Leu Cys Arg Glu Gly Val Arg Trp Arg Val Met Ala Ile Gln 
                85                  90                  95      


Asp Phe Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro Ile Ser Leu 
            100                 105                 110         


Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser Trp Glu Ile 
        115                 120                 125             


Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe Glu Ala Arg 
    130                 135                 140                 


Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu Leu Thr Leu 
145                 150                 155                 160 


Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr Pro Asp Thr 
                165                 170                 175     


Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly Glu Phe Thr 
            180                 185                 190         


Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr Lys Pro Ala 
        195                 200                 205             


Ala Leu Gly Lys Asp Thr 
    210                 


<210> 4
<211> 347
<212> PRT
<213> Homo sapiens

<400> 4
Leu Asn Thr Thr Ile Leu Thr Pro Asn Gly Asn Glu Asp Thr Thr Ala 
1               5                   10                  15      


Asp Phe Phe Leu Thr Thr Met Pro Thr Asp Ser Leu Ser Val Ser Thr 
            20                  25                  30          


Leu Pro Leu Pro Glu Val Gln Cys Phe Val Phe Asn Val Glu Tyr Met 
        35                  40                  45              


Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro Gln Pro Thr Asn Leu Thr 
    50                  55                  60                  


Leu His Tyr Trp Tyr Lys Asn Ser Asp Asn Asp Lys Val Gln Lys Cys 
65                  70                  75                  80  


Ser His Tyr Leu Phe Ser Glu Glu Ile Thr Ser Gly Cys Gln Leu Gln 
                85                  90                  95      


Lys Lys Glu Ile His Leu Tyr Gln Thr Phe Val Val Gln Leu Gln Asp 
            100                 105                 110         


Pro Arg Glu Pro Arg Arg Gln Ala Thr Gln Met Leu Lys Leu Gln Asn 
        115                 120                 125             


Leu Val Ile Pro Trp Ala Pro Glu Asn Leu Thr Leu His Lys Leu Ser 
    130                 135                 140                 


Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn Arg Phe Leu Asn His Cys 
145                 150                 155                 160 


Leu Glu His Leu Val Gln Tyr Arg Thr Asp Trp Asp His Ser Trp Thr 
                165                 170                 175     


Glu Gln Ser Val Asp Tyr Arg His Lys Phe Ser Leu Pro Ser Val Asp 
            180                 185                 190         


Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg Ser Arg Phe Asn Pro Leu 
        195                 200                 205             


Cys Gly Ser Ala Gln His Trp Ser Glu Trp Ser His Pro Ile His Trp 
    210                 215                 220                 


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
225                 230                 235                 240 


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
                245                 250                 255     


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
            260                 265                 270         


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
        275                 280                 285             


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
    290                 295                 300                 


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
305                 310                 315                 320 


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
                325                 330                 335     


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
            340                 345         


<210> 5
<211> 133
<212> PRT
<213> Homo sapiens

<400> 5
Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Asp Ile Cys Leu Pro Arg Trp Gly Cys Leu Trp 
1               5                   10      


<210> 7
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
gatggattac cttttgtgag agcatcatct caaca                                  35


<210> 8
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
tgttgagatg atgctctcac aaaaggtaat ccatc                                  35


<210> 9
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
ggattacctt ttgtatgagc atcatctcaa c                                      31


<210> 10
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
gttgagatga tgctcataca aaaggtaatc c                                      31


<210> 11
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
ggattacctt ttgtgtgagc atcatctcaa cac                                    33


<210> 12
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
gtgttgagat gatgctcaca caaaaggtaa tcc                                    33


<210> 13
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
ggattacctt ttgtctgagc atcatctcaa cac                                    33


<210> 14
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
gtgttgagat gatgctcaga caaaaggtaa tcc                                    33


<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
ggattacctt ttgtttcagc atcatctcaa cac                                    33


<210> 16
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
gtgttgagat gatgctgaaa caaaaggtaa tcc                                    33


<210> 17
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
ggattacctt ttgtaacagc atcatctcaa cac                                    33


<210> 18
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
gtgttgagat gatgctgtta caaaaggtaa tcc                                    33


<210> 19
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ggattacctt ttgtaggagc atcatctcaa cac                                    33


<210> 20
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
gtgttgagat gatgctccta caaaaggtaa tcc                                    33


<210> 21
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
ggattacctt ttgttacagc atcatctcaa cac                                    33


<210> 22
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
gtgttgagat gatgctgtaa caaaaggtaa tcc                                    33


<210> 23
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
ggattacctt ttgtaagagc atcatctc                                          28


<210> 24
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 25
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
ggattacctt ttgtaagagc atcatctc                                          28


<210> 26
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 27
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
ggattacctt ttgtaagagc atcatctc                                          28


<210> 28
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 29
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
ggattacctt ttgtaagagc atcatctc                                          28


<210> 30
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 31
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
ggattacctt ttgtaagagc atcatctc                                          28


<210> 32
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 33
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
ggattacctt ttgtaagagc atcatctc                                          28


<210> 34
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 35
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
gatggattac cttttgtgag agcatcatct caaca                                  35


<210> 36
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
tgttgagatg atgctctcac aaaaggtaat ccatc                                  35


<210> 37
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
ggattacctt ttgtatgagc atcatctcaa c                                      31


<210> 38
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
gttgagatga tgctcataca aaaggtaatc c                                      31


<210> 39
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
ggattacctt ttgtgtgagc atcatctcaa cac                                    33


<210> 40
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
gtgttgagat gatgctcaca caaaaggtaa tcc                                    33


<210> 41
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
ggattacctt ttgtctgagc atcatctcaa cac                                    33


<210> 42
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
gtgttgagat gatgctcaga caaaaggtaa tcc                                    33


<210> 43
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
ggattacctt ttgtttcagc atcatctcaa cac                                    33


<210> 44
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
gtgttgagat gatgctgaaa caaaaggtaa tcc                                    33


<210> 45
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 45
ggattacctt ttgtaacagc atcatctcaa cac                                    33


<210> 46
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 46
gtgttgagat gatgctgtta caaaaggtaa tcc                                    33


<210> 47
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 47
ggattacctt ttgtaggagc atcatctcaa cac                                    33


<210> 48
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 48
gtgttgagat gatgctccta caaaaggtaa tcc                                    33


<210> 49
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 49
ggattacctt ttgttacagc atcatctcaa cac                                    33


<210> 50
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 50
gtgttgagat gatgctgtaa caaaaggtaa tcc                                    33


<210> 51
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 51
gacttaatca gccgtatcaa cgtaata                                           27


<210> 52
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 52
tattacgttg atacggctga ttaagtc                                           27


<210> 53
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 53
ggacttaatc agcgatatca acgtaat                                           27


<210> 54
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 54
attacgttga tatcgctgat taagtcc                                           27


<210> 55
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 55
gggacttaat cagcggtatc aacgtaat                                          28


<210> 56
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 56
attacgttga taccgctgat taagtccc                                          28


<210> 57
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 57
ggacttaatc agcattatca acgtaat                                           27


<210> 58
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 58
attacgttga taatgctgat taagtcc                                           27


<210> 59
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 59
gcatttaagg ctgattttag agatgatttt g                                      31


<210> 60
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 60
caaaatcatc tctaaaatca gccttaaatg c                                      31


<210> 61
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 61
gagcatttaa ggctgcattt agagatg                                           27


<210> 62
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 62
catctctaaa tgcagcctta aatgctc                                           27


<210> 63
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 63
gcatttaagg ctgactttag agatgatttt g                                      31


<210> 64
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 64
caaaatcatc tctaaagtca gccttaaatg c                                      31


<210> 65
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 65
gcatttaagg ctgggtttag agatga                                            26


<210> 66
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 66
tcatctctaa acccagcctt aaatgc                                            26


<210> 67
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 67
gcatttaagg ctggctttag agatgatttt g                                      31


<210> 68
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 68
caaaatcatc tctaaagcca gccttaaatg c                                      31


<210> 69
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 69
cagcaatatc aacaagatag ttctggaac                                         29


<210> 70
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 70
gttccagaac tatcttgttg atattgctg                                         29


<210> 71
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 71
cagcaatatc aacaagatag ttctggaac                                         29


<210> 72
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 72
gttccagaac tatcttgttg atattgctg                                         29


<210> 73
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 73
cagcaatatc aacaagatag ttctggaac                                         29


<210> 74
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 74
gttccagaac tatcttgttg atattgctg                                         29


<210> 75
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 75
cagcaatatc aacaagatag ttctggaac                                         29


<210> 76
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 76
gttccagaac tatcttgttg atattgctg                                         29


<210> 77
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 77
cagcaatatc aacaagatag ttctggaac                                         29


<210> 78
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 78
gttccagaac tatcttgttg atattgctg                                         29


<210> 79
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 79
cagcaatatc aacaagatag ttctggaac                                         29


<210> 80
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 80
gttccagaac tatcttgttg atattgctg                                         29


<210> 81
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 81
cagcaatatc aacaagatag ttctggaac                                         29


<210> 82
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 82
gttccagaac tatcttgttg atattgctg                                         29


<210> 83
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 83
ggattacctt ttgtaagagc atcatctc                                          28


<210> 84
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 84
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 85
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 85
ggattacctt ttgtaagagc atcatctc                                          28


<210> 86
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 86
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 87
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 87
ggattacctt ttgtaagagc atcatctc                                          28


<210> 88
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 88
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 89
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 89
ggattacctt ttgtaagagc atcatctc                                          28


<210> 90
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 90
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 91
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 91
ggattacctt ttgtaagagc atcatctc                                          28


<210> 92
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 92
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 93
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 93
ggattacctt ttgtaagagc atcatctc                                          28


<210> 94
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 94
gagatgatgc tcttacaaaa ggtaatcc                                          28


<210> 95
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 95
gacttaatca gccgtatcaa cgtaata                                           27


<210> 96
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 96
tattacgttg atacggctga ttaagtc                                           27


<210> 97
<211> 133
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> A or absent

<220>
<221> MOD_RES
<222> (2)..(2)
<223> P or absent

<220>
<221> MOD_RES
<222> (3)..(3)
<223> T, C, A, G, Q, E, N, D, R, K, P, or absent

<220>
<221> MOD_RES
<222> (4)..(4)
<223> S or absent

<220>
<221> MOD_RES
<222> (5)..(5)
<223> S or absent

<220>
<221> MOD_RES
<222> (6)..(6)
<223> S or absent

<220>
<221> MOD_RES
<222> (7)..(7)
<223> T or absent

<220>
<221> MOD_RES
<222> (8)..(8)
<223> K or absent

<220>
<221> MOD_RES
<222> (9)..(9)
<223> K or absent

<220>
<221> MOD_RES
<222> (18)..(18)
<223> L, R, G, M, F, E, H, W, K, Q, S, V, I, Y, H, D, or T 

<220>
<221> MOD_RES
<222> (22)..(22)
<223> Q, F, E, G, A, L, M, F, W, K, S, V, I, Y, H, R, N, D, T, or F 

<220>
<221> MOD_RES
<222> (35)..(35)
<223> K or E 

<220>
<221> MOD_RES
<222> (38)..(38)
<223> R, W, or G 

<220>
<221> MOD_RES
<222> (39)..(39)
<223> M, L, or V 

<220>
<221> MOD_RES
<222> (55)..(55)
<223> H or Y 

<220>
<221> MOD_RES
<222> (69)..(69)
<223> V or A 

<220>
<221> MOD_RES
<222> (74)..(74)
<223> Q, P, N, H, or S 

<220>
<221> MOD_RES
<222> (80)..(80)
<223> L, F, or V 

<220>
<221> MOD_RES
<222> (81)..(81)
<223> R, I, D, or T 

<220>
<221> MOD_RES
<222> (85)..(85)
<223> L or V 

<220>
<221> MOD_RES
<222> (86)..(86)
<223> I or V 

<220>
<221> MOD_RES
<222> (89)..(89)
<223> I or V 

<220>
<221> MOD_RES
<222> (91)..(91)
<223> V, R, or K

<220>
<221> MOD_RES
<222> (92)..(92)
<223> I or F 

<220>
<221> MOD_RES
<222> (97)..(97)
<223> K or Q 

<220>
<221> MOD_RES
<222> (104)..(104)
<223> M or A 

<220>
<221> MOD_RES
<222> (109)..(109)
<223> D, C, or a non-natural amino acid with an activated side chain 

<220>
<221> MOD_RES
<222> (113)..(113)
<223> T or N 

<220>
<221> MOD_RES
<222> (125)..(125)
<223> C, A, or S 

<220>
<221> MOD_RES
<222> (126)..(126)
<223> Q, H, M, K, C, D, E, G, I, R, S, or T

<220>
<221> MOD_RES
<222> (130)..(130)
<223> S, T, G, or R

<400> 97
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Xaa Leu Asp Leu Xaa Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Xaa Leu Thr Xaa Xaa Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys Xaa Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Xaa Leu Asn Leu Ala Xaa Ser Lys Asn Phe His Xaa 
65                  70                  75                  80  


Xaa Pro Arg Asp Xaa Xaa Ser Asn Xaa Asn Xaa Xaa Val Leu Glu Leu 
                85                  90                  95      


Xaa Gly Ser Glu Thr Thr Phe Xaa Cys Glu Tyr Ala Xaa Glu Thr Ala 
            100                 105                 110         


Xaa Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Xaa Xaa Ser Ile 
        115                 120                 125             


Ile Xaa Thr Leu Thr 
    130             


<210> 98
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      His tag


<220>
<221> SITE
<222> (1)..(6) 
<223> This sequence may encompass 3-6 residues

<400> 98
His His His His His His 
1               5       


<210> 99
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 99
His His His His His His 
1               5       


<210> 100
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 100
tatagtcagc gccacccatg tacaggatgc aactcctgtc                             40


<210> 101
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 101
tatagggccc tatcaagtca gtgttgagat g                                      31


