                         SEQUENCE LISTING

<110>  Research Foundation of the City University of New York
 
<120>  TETO TO INCREASE ODORANT RECEPTOR REPRESENTATION IN THE OLFACTORY
        SYSTEM

<130>  03284.0277WO01

<140>  PCT/US21/013462
<141>  2021-01-14

<150>  US62/960,962
<151>  2020-01-14

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  19bp inverted repeat (tetracycline operator, TetO)

<400>  1
tccctatcag tgatagaga                                                    19


<210>  2
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  42 bp sequence comprising TetO

<400>  2
tcgagtttac cactccctat cagtgataga gaaaagtgaa ag                          42


<210>  3
<211>  294
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seven repeats of the 42 bp sequence comprising TetO

<400>  3
tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct       60

atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag      120

tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca      180

ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga      240

gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaag            294


<210>  4
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV mini promoter

<400>  4
gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac cg               52


<210>  5
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  42 bp sequence comprising TetO and CMV mini promoter

<400>  5
tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct       60

atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag      120

tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca      180

ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga      240

gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc      300

tcggtacccg ggtcgagtag gcgtgtacgg tgggaggcct atataagcag agctcgttta      360

gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac      420

cgggaccgat ccagcctccg cggcc                                            445


<210>  6
<211>  1008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tetracycline transactivator (tTA)

<400>  6
atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc       60

ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca      120

ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta      180

gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt      240

aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat      300

ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta      360

tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt      420

actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca      480

cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa      540

ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa      600

cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa ttacgggtct      660

accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc ggggctggcg      720

gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac ggcccccccg      780

accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat      840

gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg      900

ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt      960

gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggtag                  1008


<210>  7
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tTA advanced

<400>  7
atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatgaagtc       60

ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc      120

ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctggcaat cgagatgctg      180

gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg      240

aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat      300

ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg      360

tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt      420

acactgggct gcgtattgga ggatcaggag catcaagtag caaaagagga aagagagaca      480

cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccatcag      540

ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag      600

ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc      660

ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat      720

ttggaccttg acatgctccc cgggtaa                                          747


<210>  8
<211>  1008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse tetracycline transactivator (rtTA)

<400>  8
atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc       60

ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc ttggtgtaga gcagcctaca      120

ctgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta      180

gataggcacc atactcactt ttgcccttta aaaggggaaa gctggcaaga ttttttacgc      240

aataacgcta aaagttttag atgtgcttta ctaagtcatc gcaatggagc aaaagtacat      300

tcagatacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta      360

tgccaacaag gtttttcact agagaacgcg ttatatgcac tcagcgctgt ggggcatttt      420

actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca      480

cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa      540

ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa      600

cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa ttacgggtct      660

accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc ggggctggcg      720

gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac ggcccccccg      780

accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat      840

gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg      900

ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt      960

gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggtag                  1008


<210>  9
<211>  1473
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VP16

<400>  9
atggacctct tggtcgacga gctgtttgcc gacatgaacg cggacggcgc ttcgccaccg       60

cccccccgcc cggccggggg tcccaaaaac accccggcgg cccccccgct gtacgcaacg      120

gggcgcctga gccaggccca gctcatgccc tccccaccca tgcccgtccc ccccgccgcc      180

ctctttaacc gtctcctcga cgacttgggc tttagcgcgg gccccgcgct atgtaccatg      240

ctcgatacct ggaacgagga tctgttttcg gcgctaccga ccaacgccga cctgtaccgg      300

gagtgtaaat tcctatcaac gctgcccagc gatgtggtgg aatgggggga cgcgtacgtc      360

cccgaacgca cccaaatcga cattcgcgcc cacggcgacg tggccttccc tacgcttccg      420

gccacccgcg acggcctcgg gctctactac gaagcgctct ctcgtttctt ccacgccgag      480

ctacgggcgc gggaggagag ctatcgaacc gtgttggcca acttctgctc ggccctgtac      540

cggtacctgc gcgccagcgt ccggcagctg caccgccagg cgcacatgcg cggacgcgat      600

cgcgacctgg gagaaatgct gcgcgccacg atcgcggaca ggtactaccg agagaccgct      660

cgtctggcgc gtgttttgtt tttgcatttg tatctatttt tgacccgcga gatcctatgg      720

gccgcgtacg ccgagcagat gatgcggccc gacctgtttg actgcctctg ttgcgacctg      780

gagagctggc gtcagttggc gggtctgttc cagcccttca tgttcgtcaa cggagcgctc      840

accgtccggg gagtgccaat cgaggcccgc cggctgcggg agctaaacca cattcgcgag      900

caccttaacc tcccgctggt gcgcagcgcg gctacggagg agccaggggc gccgttgacg      960

acccctccca ccctgcatgg caaccaggcc cgcgcctctg ggtactttat ggtgttgatt     1020

cgggcgaagt tggactcgta ttccagcttc acgacctcgc cctccgaggc ggtcatgcgg     1080

gaacacgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat cgagggcctg     1140

ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc gcgcctgtcc     1200

tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga tgtcagcctg     1260

ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac     1320

gatttcgatc tggacatgtt gggggacggg gattccccgg ggccgggatt taccccccac     1380

gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc     1440

gatgcccttg gaattgacga gtacggtggg tag                                  1473


<210>  10
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tTA

<400>  10

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser Ala 
        195                 200                 205             


Tyr Ser Arg Ala Arg Thr Lys Asn Asn Tyr Gly Ser Thr Ile Glu Gly 
    210                 215                 220                 


Leu Leu Asp Leu Pro Asp Asp Asp Ala Pro Glu Glu Ala Gly Leu Ala 
225                 230                 235                 240 


Ala Pro Arg Leu Ser Phe Leu Pro Ala Gly His Thr Arg Arg Leu Ser 
                245                 250                 255     


Thr Ala Pro Pro Thr Asp Val Ser Leu Gly Asp Glu Leu His Leu Asp 
            260                 265                 270         


Gly Glu Asp Val Ala Met Ala His Ala Asp Ala Leu Asp Asp Phe Asp 
        275                 280                 285             


Leu Asp Met Leu Gly Asp Gly Asp Ser Pro Gly Pro Gly Phe Thr Pro 
    290                 295                 300                 


His Asp Ser Ala Pro Tyr Gly Ala Leu Asp Met Ala Asp Phe Glu Phe 
305                 310                 315                 320 


Glu Gln Met Phe Thr Asp Ala Leu Gly Ile Asp Glu Tyr Gly Gly 
                325                 330                 335 


<210>  11
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tTA advanced

<400>  11

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Gly Pro 
        195                 200                 205             


Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Pro Ala Asp Ala 
    210                 215                 220                 


Leu Asp Asp Phe Asp Leu Asp Met Leu Pro Ala Asp Ala Leu Asp Asp 
225                 230                 235                 240 


Leu Asp Leu Asp Met Leu Pro Gly 
                245             


<210>  12
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rtTA

<400>  12

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Lys Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asn Gly 
                85                  90                  95      


Ala Lys Val His Ser Asp Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser Ala 
        195                 200                 205             


Tyr Ser Arg Ala Arg Thr Lys Asn Asn Tyr Gly Ser Thr Ile Glu Gly 
    210                 215                 220                 


Leu Leu Asp Leu Pro Asp Asp Asp Ala Pro Glu Glu Ala Gly Leu Ala 
225                 230                 235                 240 


Ala Pro Arg Leu Ser Phe Leu Pro Ala Gly His Thr Arg Arg Leu Ser 
                245                 250                 255     


Thr Ala Pro Pro Thr Asp Val Ser Leu Gly Asp Glu Leu His Leu Asp 
            260                 265                 270         


Gly Glu Asp Val Ala Met Ala His Ala Asp Ala Leu Asp Asp Phe Asp 
        275                 280                 285             


Leu Asp Met Leu Gly Asp Gly Asp Ser Pro Gly Pro Gly Phe Thr Pro 
    290                 295                 300                 


His Asp Ser Ala Pro Tyr Gly Ala Leu Asp Met Ala Asp Phe Glu Phe 
305                 310                 315                 320 


Glu Gln Met Phe Thr Asp Ala Leu Gly Ile Asp Glu Tyr Gly Gly 
                325                 330                 335 


<210>  13
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VP16

<400>  13

Met Asp Leu Leu Val Asp Glu Leu Phe Ala Asp Met Asn Ala Asp Gly 
1               5                   10                  15      


Ala Ser Pro Pro Pro Pro Arg Pro Ala Gly Gly Pro Lys Asn Thr Pro 
            20                  25                  30          


Ala Ala Pro Pro Leu Tyr Ala Thr Gly Arg Leu Ser Gln Ala Gln Leu 
        35                  40                  45              


Met Pro Ser Pro Pro Met Pro Val Pro Pro Ala Ala Leu Phe Asn Arg 
    50                  55                  60                  


Leu Leu Asp Asp Leu Gly Phe Ser Ala Gly Pro Ala Leu Cys Thr Met 
65                  70                  75                  80  


Leu Asp Thr Trp Asn Glu Asp Leu Phe Ser Ala Leu Pro Thr Asn Ala 
                85                  90                  95      


Asp Leu Tyr Arg Glu Cys Lys Phe Leu Ser Thr Leu Pro Ser Asp Val 
            100                 105                 110         


Val Glu Trp Gly Asp Ala Tyr Val Pro Glu Arg Thr Gln Ile Asp Ile 
        115                 120                 125             


Arg Ala His Gly Asp Val Ala Phe Pro Thr Leu Pro Ala Thr Arg Asp 
    130                 135                 140                 


Gly Leu Gly Leu Tyr Tyr Glu Ala Leu Ser Arg Phe Phe His Ala Glu 
145                 150                 155                 160 


Leu Arg Ala Arg Glu Glu Ser Tyr Arg Thr Val Leu Ala Asn Phe Cys 
                165                 170                 175     


Ser Ala Leu Tyr Arg Tyr Leu Arg Ala Ser Val Arg Gln Leu His Arg 
            180                 185                 190         


Gln Ala His Met Arg Gly Arg Asp Arg Asp Leu Gly Glu Met Leu Arg 
        195                 200                 205             


Ala Thr Ile Ala Asp Arg Tyr Tyr Arg Glu Thr Ala Arg Leu Ala Arg 
    210                 215                 220                 


Val Leu Phe Leu His Leu Tyr Leu Phe Leu Thr Arg Glu Ile Leu Trp 
225                 230                 235                 240 


Ala Ala Tyr Ala Glu Gln Met Met Arg Pro Asp Leu Phe Asp Cys Leu 
                245                 250                 255     


Cys Cys Asp Leu Glu Ser Trp Arg Gln Leu Ala Gly Leu Phe Gln Pro 
            260                 265                 270         


Phe Met Phe Val Asn Gly Ala Leu Thr Val Arg Gly Val Pro Ile Glu 
        275                 280                 285             


Ala Arg Arg Leu Arg Glu Leu Asn His Ile Arg Glu His Leu Asn Leu 
    290                 295                 300                 


Pro Leu Val Arg Ser Ala Ala Thr Glu Glu Pro Gly Ala Pro Leu Thr 
305                 310                 315                 320 


Thr Pro Pro Thr Leu His Gly Asn Gln Ala Arg Ala Ser Gly Tyr Phe 
                325                 330                 335     


Met Val Leu Ile Arg Ala Lys Leu Asp Ser Tyr Ser Ser Phe Thr Thr 
            340                 345                 350         


Ser Pro Ser Glu Ala Val Met Arg Glu His Ala Tyr Ser Arg Ala Arg 
        355                 360                 365             


Thr Lys Asn Asn Tyr Gly Ser Thr Ile Glu Gly Leu Leu Asp Leu Pro 
    370                 375                 380                 


Asp Asp Asp Ala Pro Glu Glu Ala Gly Leu Ala Ala Pro Arg Leu Ser 
385                 390                 395                 400 


Phe Leu Pro Ala Gly His Thr Arg Arg Leu Ser Thr Ala Pro Pro Thr 
                405                 410                 415     


Asp Val Ser Leu Gly Asp Glu Leu His Leu Asp Gly Glu Asp Val Ala 
            420                 425                 430         


Met Ala His Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly 
        435                 440                 445             


Asp Gly Asp Ser Pro Gly Pro Gly Phe Thr Pro His Asp Ser Ala Pro 
    450                 455                 460                 


Tyr Gly Ala Leu Asp Met Ala Asp Phe Glu Phe Glu Gln Met Phe Thr 
465                 470                 475                 480 


Asp Ala Leu Gly Ile Asp Glu Tyr Gly Gly 
                485                 490 


