                         SEQUENCE LISTING

<110>  The Israel Institute of Biological
Research (IIBR)
 
<120>  Methods for Identifying Anti Clostridial Neurotoxin
Compounds

<130>  2757730

<150>  IL272002
<151>  2020-01-13

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  1024
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
1               5                   10                  15      


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
            20                  25                  30          


Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro 
        35                  40                  45              


Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe 
    50                  55                  60                  


Pro Ala Pro Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro 
65                  70                  75                  80  


Glu Ala Asp Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly Tyr 
                85                  90                  95      


Asp Ala Pro Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro 
            100                 105                 110         


Pro Phe Val Pro Thr Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr Phe 
        115                 120                 125             


Asn Val Asp Glu Ser Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile Phe 
    130                 135                 140                 


Asp Gly Val Asn Ser Ala Phe His Leu Trp Cys Asn Gly Arg Trp Val 
145                 150                 155                 160 


Gly Tyr Gly Gln Asp Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser Ala 
                165                 170                 175     


Phe Leu Arg Ala Gly Glu Asn Arg Leu Ala Val Met Val Leu Arg Trp 
            180                 185                 190         


Ser Asp Gly Ser Tyr Leu Glu Asp Gln Asp Met Trp Arg Met Ser Gly 
        195                 200                 205             


Ile Phe Arg Asp Val Ser Leu Leu His Lys Pro Thr Thr Gln Ile Ser 
    210                 215                 220                 


Asp Phe His Val Ala Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala Val 
225                 230                 235                 240 


Leu Glu Ala Glu Val Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu Arg 
                245                 250                 255     


Val Thr Val Ser Leu Trp Gln Gly Glu Thr Gln Val Ala Ser Gly Thr 
            260                 265                 270         


Ala Pro Phe Gly Gly Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala Asp 
        275                 280                 285             


Arg Val Thr Leu Arg Leu Asn Val Glu Asn Pro Lys Leu Trp Ser Ala 
    290                 295                 300                 


Glu Ile Pro Asn Leu Tyr Arg Ala Val Val Glu Leu His Thr Ala Asp 
305                 310                 315                 320 


Gly Thr Leu Ile Glu Ala Glu Ala Cys Asp Val Gly Phe Arg Glu Val 
                325                 330                 335     


Arg Ile Glu Asn Gly Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile 
            340                 345                 350         


Arg Gly Val Asn Arg His Glu His His Pro Leu His Gly Gln Val Met 
        355                 360                 365             


Asp Glu Gln Thr Met Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn 
    370                 375                 380                 


Phe Asn Ala Val Arg Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr 
385                 390                 395                 400 


Thr Leu Cys Asp Arg Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile 
                405                 410                 415     


Glu Thr His Gly Met Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg 
            420                 425                 430         


Trp Leu Pro Ala Met Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp 
        435                 440                 445             


Arg Asn His Pro Ser Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly 
    450                 455                 460                 


His Gly Ala Asn His Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp 
465                 470                 475                 480 


Pro Ser Arg Pro Val Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala 
                485                 490                 495     


Thr Asp Ile Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro 
            500                 505                 510         


Phe Pro Ala Val Pro Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro 
        515                 520                 525             


Gly Glu Thr Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly 
    530                 535                 540                 


Asn Ser Leu Gly Gly Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr 
545                 550                 555                 560 


Pro Arg Leu Gln Gly Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu 
                565                 570                 575     


Ile Lys Tyr Asp Glu Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly Asp 
            580                 585                 590         


Phe Gly Asp Thr Pro Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val 
        595                 600                 605             


Phe Ala Asp Arg Thr Pro His Pro Ala Leu Thr Glu Ala Lys His Gln 
    610                 615                 620                 


Gln Gln Phe Phe Gln Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr 
625                 630                 635                 640 


Ser Glu Tyr Leu Phe Arg His Ser Asp Asn Glu Leu Leu His Trp Met 
                645                 650                 655     


Val Ala Leu Asp Gly Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp 
            660                 665                 670         


Val Ala Pro Gln Gly Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln 
        675                 680                 685             


Pro Glu Ser Ala Gly Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro 
    690                 695                 700                 


Asn Ala Thr Ala Trp Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln 
705                 710                 715                 720 


Trp Arg Leu Ala Glu Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His 
                725                 730                 735     


Ala Ile Pro His Leu Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu 
            740                 745                 750         


Gly Asn Lys Arg Trp Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln 
        755                 760                 765             


Met Trp Ile Gly Asp Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln 
    770                 775                 780                 


Phe Thr Arg Ala Pro Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr 
785                 790                 795                 800 


Arg Ile Asp Pro Asn Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His 
                805                 810                 815     


Tyr Gln Ala Glu Ala Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala 
            820                 825                 830         


Asp Ala Val Leu Ile Thr Thr Ala His Ala Trp Gln His Gln Gly Lys 
        835                 840                 845             


Thr Leu Phe Ile Ser Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln 
    850                 855                 860                 


Met Ala Ile Thr Val Asp Val Glu Val Ala Ser Asp Thr Pro His Pro 
865                 870                 875                 880 


Ala Arg Ile Gly Leu Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val 
                885                 890                 895     


Asn Trp Leu Gly Leu Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr 
            900                 905                 910         


Ala Ala Cys Phe Asp Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr 
        915                 920                 925             


Pro Tyr Val Phe Pro Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu 
    930                 935                 940                 


Leu Asn Tyr Gly Pro His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile 
945                 950                 955                 960 


Ser Arg Tyr Ser Gln Gln Gln Leu Met Glu Thr Ser His Arg His Leu 
                965                 970                 975     


Leu His Ala Glu Glu Gly Thr Trp Leu Asn Ile Asp Gly Phe His Met 
            980                 985                 990         


Gly Ile Gly Gly Asp Asp Ser Trp  Ser Pro Ser Val Ser  Ala Glu Phe 
        995                 1000                 1005             


Gln Leu  Ser Ala Gly Arg Tyr  His Tyr Gln Leu Val  Trp Cys Gln 
    1010                 1015                 1020             


Lys 
    


<210>  2
<211>  425
<212>  PRT
<213>  Clostridium botulinum

<400>  2

Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His 
1               5                   10                  15      


Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys 
            20                  25                  30          


Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu 
        35                  40                  45              


Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn 
    50                  55                  60                  


Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile 
            100                 105                 110         


Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe 
        115                 120                 125             


Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 
                165                 170                 175     


His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr 
            180                 185                 190         


His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 
        195                 200                 205             


Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr 
225                 230                 235                 240 


Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn 
                245                 250                 255     


Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val 
            260                 265                 270         


Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys 
        275                 280                 285             


Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg 
    290                 295                 300                 


Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr 
305                 310                 315                 320 


Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser 
                325                 330                 335     


Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met 
            340                 345                 350         


Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu 
        355                 360                 365             


Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe 
    370                 375                 380                 


Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile 
385                 390                 395                 400 


Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val 
                405                 410                 415     


Asp Asp Gly Trp Gly Glu Arg Pro Leu 
            420                 425 


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 1

<400>  3

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6His tag

<400>  4

His His His His His His 
1               5       


<210>  5
<211>  1472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   beta-gal-HC/A fusion protein construct

<400>  5

Met Lys Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg 
1               5                   10                  15      


Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His 
            20                  25                  30          


Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
        35                  40                  45              


Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp 
    50                  55                  60                  


Phe Pro Ala Pro Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu 
65                  70                  75                  80  


Pro Glu Ala Asp Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly 
                85                  90                  95      


Tyr Asp Ala Pro Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn 
            100                 105                 110         


Pro Pro Phe Val Pro Thr Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr 
        115                 120                 125             


Phe Asn Val Asp Glu Ser Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile 
    130                 135                 140                 


Phe Asp Gly Val Asn Ser Ala Phe His Leu Trp Cys Asn Gly Arg Trp 
145                 150                 155                 160 


Val Gly Tyr Gly Gln Asp Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser 
                165                 170                 175     


Ala Phe Leu Arg Ala Gly Glu Asn Arg Leu Ala Val Met Val Leu Arg 
            180                 185                 190         


Trp Ser Asp Gly Ser Tyr Leu Glu Asp Gln Asp Met Trp Arg Met Ser 
        195                 200                 205             


Gly Ile Phe Arg Asp Val Ser Leu Leu His Lys Pro Thr Thr Gln Ile 
    210                 215                 220                 


Ser Asp Phe His Val Ala Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala 
225                 230                 235                 240 


Val Leu Glu Ala Glu Val Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu 
                245                 250                 255     


Arg Val Thr Val Ser Leu Trp Gln Gly Glu Thr Gln Val Ala Ser Gly 
            260                 265                 270         


Thr Ala Pro Phe Gly Gly Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala 
        275                 280                 285             


Asp Arg Val Thr Leu Arg Leu Asn Val Glu Asn Pro Lys Leu Trp Ser 
    290                 295                 300                 


Ala Glu Ile Pro Asn Leu Tyr Arg Ala Val Val Glu Leu His Thr Ala 
305                 310                 315                 320 


Asp Gly Thr Leu Ile Glu Ala Glu Ala Cys Asp Val Gly Phe Arg Glu 
                325                 330                 335     


Val Arg Ile Glu Asn Gly Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu 
            340                 345                 350         


Ile Arg Gly Val Asn Arg His Glu His His Pro Leu His Gly Gln Val 
        355                 360                 365             


Met Asp Glu Gln Thr Met Val Gln Asp Ile Leu Leu Met Lys Gln Asn 
    370                 375                 380                 


Asn Phe Asn Ala Val Arg Cys Ser His Tyr Pro Asn His Pro Leu Trp 
385                 390                 395                 400 


Tyr Thr Leu Cys Asp Arg Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn 
                405                 410                 415     


Ile Glu Thr His Gly Met Val Pro Met Asn Arg Leu Thr Asp Asp Pro 
            420                 425                 430         


Arg Trp Leu Pro Ala Met Ser Glu Arg Val Thr Arg Met Val Gln Arg 
        435                 440                 445             


Asp Arg Asn His Pro Ser Val Ile Ile Trp Ser Leu Gly Asn Glu Ser 
    450                 455                 460                 


Gly His Gly Ala Asn His Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val 
465                 470                 475                 480 


Asp Pro Ser Arg Pro Val Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr 
                485                 490                 495     


Ala Thr Asp Ile Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln 
            500                 505                 510         


Pro Phe Pro Ala Val Pro Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu 
        515                 520                 525             


Pro Gly Glu Thr Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Ala Met 
    530                 535                 540                 


Gly Asn Ser Leu Gly Gly Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln 
545                 550                 555                 560 


Tyr Pro Arg Leu Gln Gly Gly Phe Val Trp Asp Trp Val Asp Gln Ser 
                565                 570                 575     


Leu Ile Lys Tyr Asp Glu Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly 
            580                 585                 590         


Asp Phe Gly Asp Thr Pro Asn Asp Arg Gln Phe Cys Met Asn Gly Leu 
        595                 600                 605             


Val Phe Ala Asp Arg Thr Pro His Pro Ala Leu Thr Glu Ala Lys His 
    610                 615                 620                 


Gln Gln Gln Phe Phe Gln Phe Arg Leu Ser Gly Gln Thr Ile Glu Val 
625                 630                 635                 640 


Thr Ser Glu Tyr Leu Phe Arg His Ser Asp Asn Glu Leu Leu His Trp 
                645                 650                 655     


Met Val Ala Leu Asp Gly Lys Pro Leu Ala Ser Gly Glu Val Pro Leu 
            660                 665                 670         


Asp Val Ala Pro Gln Gly Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro 
        675                 680                 685             


Gln Pro Glu Ser Ala Gly Gln Leu Trp Leu Thr Val Arg Val Val Gln 
    690                 695                 700                 


Pro Asn Ala Thr Ala Trp Ser Glu Ala Gly His Ile Ser Ala Trp Gln 
705                 710                 715                 720 


Gln Trp Arg Leu Ala Glu Asn Leu Ser Val Thr Leu Pro Ala Ala Ser 
                725                 730                 735     


His Ala Ile Pro His Leu Thr Thr Ser Glu Met Asp Phe Cys Ile Glu 
            740                 745                 750         


Leu Gly Asn Lys Arg Trp Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser 
        755                 760                 765             


Gln Met Trp Ile Gly Asp Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp 
    770                 775                 780                 


Gln Phe Thr Arg Ala Pro Leu Asp Asn Asp Ile Gly Val Ser Glu Ala 
785                 790                 795                 800 


Thr Arg Ile Asp Pro Asn Ala Trp Val Glu Arg Trp Lys Ala Ala Gly 
                805                 810                 815     


His Tyr Gln Ala Glu Ala Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu 
            820                 825                 830         


Ala Asp Ala Val Leu Ile Thr Thr Ala His Ala Trp Gln His Gln Gly 
        835                 840                 845             


Lys Thr Leu Phe Ile Ser Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly 
    850                 855                 860                 


Gln Met Ala Ile Thr Val Asp Val Glu Val Ala Ser Asp Thr Pro His 
865                 870                 875                 880 


Pro Ala Arg Ile Gly Leu Asn Cys Gln Leu Ala Gln Val Ala Glu Arg 
                885                 890                 895     


Val Asn Trp Leu Gly Leu Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu 
            900                 905                 910         


Thr Ala Ala Cys Phe Asp Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr 
        915                 920                 925             


Thr Pro Tyr Val Phe Pro Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg 
    930                 935                 940                 


Glu Leu Asn Tyr Gly Pro His Gln Trp Arg Gly Asp Phe Gln Phe Asn 
945                 950                 955                 960 


Ile Ser Arg Tyr Ser Gln Gln Gln Leu Met Glu Thr Ser His Arg His 
                965                 970                 975     


Leu Leu His Ala Glu Glu Gly Thr Trp Leu Asn Ile Asp Gly Phe His 
            980                 985                 990         


Met Gly Ile Gly Gly Asp Asp Ser  Trp Ser Pro Ser Val  Ser Ala Glu 
        995                 1000                 1005             


Phe Gln  Leu Ser Ala Gly Arg  Tyr His Tyr Gln Leu  Val Trp Cys 
    1010                 1015                 1020             


Gln Lys  Gly Gly Gly Gly Ser  Gly Gly Gly Gly Ser  Gly Gly Gly 
    1025                 1030                 1035             


Gly Ser  Met Asn Ile Ile Asn  Thr Ser Ile Leu Asn  Leu Arg Tyr 
    1040                 1045                 1050             


Glu Ser  Asn His Leu Ile Asp  Leu Ser Arg Tyr Ala  Ser Lys Ile 
    1055                 1060                 1065             


Asn Ile  Gly Ser Lys Val Asn  Phe Asp Pro Ile Asp  Lys Asn Gln 
    1070                 1075                 1080             


Ile Gln  Leu Phe Asn Leu Glu  Ser Ser Lys Ile Glu  Val Ile Leu 
    1085                 1090                 1095             


Lys Asn  Ala Ile Val Tyr Asn  Ser Met Tyr Glu Asn  Phe Ser Thr 
    1100                 1105                 1110             


Ser Phe  Trp Ile Arg Ile Pro  Lys Tyr Phe Asn Ser  Ile Ser Leu 
    1115                 1120                 1125             


Asn Asn  Glu Tyr Thr Ile Ile  Asn Cys Met Glu Asn  Asn Ser Gly 
    1130                 1135                 1140             


Trp Lys  Val Ser Leu Asn Tyr  Gly Glu Ile Ile Trp  Thr Leu Gln 
    1145                 1150                 1155             


Asp Thr  Gln Glu Ile Lys Gln  Arg Val Val Phe Lys  Tyr Ser Gln 
    1160                 1165                 1170             


Met Ile  Asn Ile Ser Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr 
    1175                 1180                 1185             


Ile Thr  Asn Asn Arg Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly 
    1190                 1195                 1200             


Arg Leu  Ile Asp Gln Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His 
    1205                 1210                 1215             


Ala Ser  Asn Asn Ile Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr 
    1220                 1225                 1230             


His Arg  Tyr Ile Trp Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu 
    1235                 1240                 1245             


Leu Asn  Glu Lys Glu Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn 
    1250                 1255                 1260             


Ser Gly  Ile Leu Lys Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp 
    1265                 1270                 1275             


Lys Pro  Tyr Tyr Met Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val 
    1280                 1285                 1290             


Asp Val  Asn Asn Val Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly 
    1295                 1300                 1305             


Pro Arg  Gly Ser Val Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser 
    1310                 1315                 1320             


Leu Tyr  Arg Gly Thr Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly 
    1325                 1330                 1335             


Asn Lys  Asp Asn Ile Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn 
    1340                 1345                 1350             


Val Val  Val Lys Asn Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser 
    1355                 1360                 1365             


Gln Ala  Gly Val Glu Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp 
    1370                 1375                 1380             


Val Gly  Asn Leu Ser Gln Val  Val Val Met Lys Ser  Lys Asn Asp 
    1385                 1390                 1395             


Gln Gly  Ile Thr Asn Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn 
    1400                 1405                 1410             


Gly Asn  Asp Ile Gly Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile 
    1415                 1420                 1425             


Ala Lys  Leu Val Ala Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg 
    1430                 1435                 1440             


Ser Ser  Arg Thr Leu Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp 
    1445                 1450                 1455             


Asp Gly  Trp Gly Glu Arg Pro  Leu His His His His  His His 
    1460                 1465                 1470         


<210>  6
<211>  3075
<212>  DNA
<213>  Escherichia coli

<400>  6
atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct       60

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc      120

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc      180

tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct      240

gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc      300

tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg      360

acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg      420

cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc      480

ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc      540

ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat      600

caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact      660

acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta      720

ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct      780

ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc      840

gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa      900

ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac      960

ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat     1020

ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat     1080

catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg     1140

aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac     1200

acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc     1260

atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc     1320

gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg     1380

aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat     1440

ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt     1500

tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc     1560

atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc     1620

cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat     1680

ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat     1740

gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc     1800

cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa     1860

gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc     1920

agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat     1980

ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg     2040

attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc     2100

gtagtgcaac cgaacgcgac cgcatggtca gaagccggac acatcagcgc ctggcagcag     2160

tggcgtctgg ctgaaaacct cagcgtgaca ctccccgccg cgtcccacgc catcccgcat     2220

ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac     2280

cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg     2340

ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc     2400

cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa     2460

gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct     2520

cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat     2580

ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg     2640

gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga     2700

ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat     2760

ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc     2820

gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc     2880

agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa     2940

gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg     3000

agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc     3060

tggtgtcaaa aataa                                                      3075


<210>  7
<211>  3078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequence encoding E.coli beta-gal

<400>  7
catatgaaaa ccatgattac ggatagcctg gccgtggttc tgcaacgtcg cgactgggaa       60

aatccgggtg tgacccagct gaaccgtctg gccgcacatc cgccgtttgc tagttggcgc      120

aactccgaag aagcgcgtac ggatcgcccg agccagcaac tgcgttctct gaatggcgaa      180

tggcgctttg cctggtttcc ggccccggaa gccgttccgg aaagctggct ggaatgcgat      240

ctgccggaag ctgacaccgt cgtggttccg tctaactggc agatgcatgg ttatgatgcg      300

ccgatctaca ccaatgtgac gtatccgatt acggtgaacc cgccgttcgt tccgaccgaa      360

aatccgacgg gctgctatag cctgaccttt aatgttgatg aatcttggct gcaggaaggt      420

caaacgcgta ttatcttcga cggcgttaac agtgcctttc acctgtggtg taatggtcgt      480

tgggtcggtt acggccagga ttcacgcctg ccgtcggaat ttgacctgtc cgcttttctg      540

cgtgcgggcg aaaaccgcct ggcagttatg gtcctgcgct ggtcagatgg ttcgtatctg      600

gaagatcagg acatgtggcg tatgtcaggc atcttccgcg atgtgtcgct gctgcataaa      660

ccgaccacgc agatttcaga ctttcacgtt gcgacccgtt tcaatgatga cttttcgcgc      720

gccgtcctgg aagcagaagt gcagatgtgc ggtgaactgc gtgattatct gcgcgtgacg      780

gtttcactgt ggcagggtga aacccaggtg gctagcggca cggccccgtt tggcggtgaa      840

attatcgatg aacgtggcgg ttacgcggac cgtgtgaccc tgcgtctgaa cgttgaaaat      900

ccgaaactgt ggagcgcaga aattccgaac ctgtatcgcg ctgtcgtgga actgcatacc      960

gcggatggta cgctgatcga agcagaagct tgtgacgttg gcttccgtga agtccgcatt     1020

gaaaatggtc tgctgctgct gaacggcaaa ccgctgctga tccgtggtgt gaatcgccat     1080

gaacatcacc cgctgcacgg ccaggtgatg gatgaacaga cgatggtgca agacattctg     1140

ctgatgaaac agaacaattt taacgcggtt cgttgcagtc attacccgaa tcacccgctg     1200

tggtataccc tgtgtgatcg ctatggtctg tacgttgtcg acgaagcgaa catcgaaacc     1260

cacggcatgg tgccgatgaa tcgtctgacc gatgacccgc gctggctgcc ggcaatgagc     1320

gaacgtgtta cccgcatggt ccagcgtgat cgcaaccacc cgagtgttat tatctggtcc     1380

ctgggcaatg aatcaggtca tggcgccaac cacgatgcac tgtaccgttg gattaaaagc     1440

gtcgacccgt ctcgcccggt gcagtatgaa ggcggtggcg ctgataccac cgccaccgac     1500

attatctgcc cgatgtacgc tcgtgttgat gaagaccagc cgtttccggc ggtcccgaaa     1560

tggtcaatca aaaaatggct gagcctgccg ggtgaaaccc gtccgctgat cctgtgtgaa     1620

tatgcgcatg cgatgggcaa cagcctgggt ggcttcgcca aatactggca ggcatttcgt     1680

caatatccgc gcctgcaagg tggcttcgtg tgggattggg ttgaccagag tctgattaaa     1740

tacgatgaaa acggtaatcc gtggtccgcg tatggcggcg attttggcga caccccgaat     1800

gatcgtcagt tctgcatgaa cggtctggtg tttgccgacc gtaccccgca tccggcactg     1860

accgaagcaa aacaccagca acagtttttc cagttccgtc tgtctggcca aaccattgaa     1920

gtgacgagcg aatacctgtt tcgccattct gataacgaac tgctgcactg gatggttgcc     1980

ctggacggta aaccgctggc atcaggcgaa gtcccgctgg atgtggctcc gcagggtaaa     2040

caactgattg aactgccgga actgccgcag ccggaaagcg caggccaact gtggctgacc     2100

gtgcgtgtgg ttcagccgaa cgcaacggct tggagcgaag ctggtcatat ctctgcgtgg     2160

caacagtggc gcctggccga aaatctgagt gttaccctgc cggcagcttc ccatgcaatc     2220

ccgcacctga ccacgagcga aatggatttc tgtattgaac tgggcaataa acgttggcag     2280

ttcaaccgcc aaagtggttt tctgtcccag atgtggattg gcgataaaaa acaactgctg     2340

accccgctgc gtgaccagtt tacccgtgcc ccgctggata acgacatcgg tgtgtctgaa     2400

gctacccgta ttgatccgaa tgcgtgggtt gaacgctgga aagcggccgg ccattaccag     2460

gccgaagcag ctctgctgca atgtaccgca gataccctgg ccgatgcggt gctgattacc     2520

acggcacatg cttggcagca ccaaggtaaa accctgttta tcagccgtaa aacgtatcgc     2580

attgatggtt ctggccagat ggcgattacc gtcgatgtgg aagttgcaag cgacacgccg     2640

cacccggcac gtattggtct gaattgtcag ctggcacaag ttgctgaacg tgtcaactgg     2700

ctgggtctgg gtccgcagga aaattatccg gatcgtctga ccgccgcatg ctttgatcgt     2760

tgggacctgc cgctgagcga tatgtatacc ccgtacgtgt ttccgtctga aaacggtctg     2820

cgttgtggca cgcgcgaact gaattacggt ccgcatcagt ggcgtggcga ttttcaattc     2880

aacattagcc gctattctca acagcaactg atggaaacca gccatcgtca cctgctgcat     2940

gcggaagaag gtacgtggct gaatatcgat ggtttccaca tgggcattgg tggcgatgac     3000

agctggtctc cgagtgtttc cgccgaattt cagctgtctg caggccgcta tcattaccag     3060

ctggtctggt gtcaaaaa                                                   3078


<210>  8
<211>  1296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/A HC fragment

<400>  8
aacattatca ataccagtat cctgaatctg cgttacgaat ccaaccacct gattgatctg       60

tcacgctatg cctcgaaaat caacatcggt tcaaaagtga acttcgatcc gatcgacaaa      120

aatcagattc aactgtttaa cctggaaagc tctaaaattg aagtcatcct gaaaaacgcg      180

attgtgtata attccatgta cgaaaacttc tcaacctcgt tttggattcg tatcccgaaa      240

tactttaaca gcatctctct gaacaacgaa tacacgatca tcaactgcat ggaaaacaat      300

agtggttgga aagtgtccct gaactatggc gaaattatct ggaccctgca ggatacgcaa      360

gaaattaaac aacgtgtcgt gttcaaatac agtcagatga tcaacatctc cgattacatc      420

aaccgttgga tctttgttac cattacgaac aatcgcctga acaacagcaa aatctacatc      480

aacggtcgcc tgatcgatca gaaaccgatt tcaaacctgg gcaatatcca tgcgtcgaac      540

aatattatgt tcaaactgga tggctgtcgt gacacccacc gctacatttg gatcaaatac      600

ttcaacctgt ttgataaaga actgaacgaa aaagaaatca aagatctgta tgacaaccag      660

agtaattccg gtattctgaa agatttttgg ggcgactatc tgcagtacga taaaccgtat      720

tacatgctga atctgtacga tccgaacaaa tatgtcgacg tgaacaatgt cggtattcgt      780

ggctatatgt acctgaaagg tccgcgcggc agcgtgatga ccacgaacat ctacctgaat      840

agttccctgt accgtggcac caaattcatc atcaaaaaat acgcctctgg caacaaagat      900

aatatcgttc gtaacaatga ccgcgtctac atcaacgttg tcgtgaaaaa caaagaatac      960

cgcctggcga ccaacgccag tcaggcaggt gtcgaaaaaa tcctgtccgc gctggaaatt     1020

ccggatgtgg gcaacctgtc acaagttgtc gtgatgaaat cgaaaaatga tcagggtatt     1080

accaataaat gcaaaatgaa cctgcaggat aacaatggca atgacattgg ttttatcggc     1140

ttccatcagt ttaacaatat cgctaaactg gtggcgagta actggtataa tcgtcagatt     1200

gaacgttcat cgcgcaccct gggctgttcc tgggaattta tcccggttga tgacggttgg     1260

ggcgaacgcc cgctgcatca ccatcaccat cactaa                               1296


<210>  9
<211>  4428
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  beta-gal-HC/A fusion protein construct

<400>  9
catatgaaaa ccatgattac ggatagcctg gccgtggttc tgcaacgtcg cgactgggaa       60

aatccgggtg tgacccagct gaaccgtctg gccgcacatc cgccgtttgc tagttggcgc      120

aactccgaag aagcgcgtac ggatcgcccg agccagcaac tgcgttctct gaatggcgaa      180

tggcgctttg cctggtttcc ggccccggaa gccgttccgg aaagctggct ggaatgcgat      240

ctgccggaag ctgacaccgt cgtggttccg tctaactggc agatgcatgg ttatgatgcg      300

ccgatctaca ccaatgtgac gtatccgatt acggtgaacc cgccgttcgt tccgaccgaa      360

aatccgacgg gctgctatag cctgaccttt aatgttgatg aatcttggct gcaggaaggt      420

caaacgcgta ttatcttcga cggcgttaac agtgcctttc acctgtggtg taatggtcgt      480

tgggtcggtt acggccagga ttcacgcctg ccgtcggaat ttgacctgtc cgcttttctg      540

cgtgcgggcg aaaaccgcct ggcagttatg gtcctgcgct ggtcagatgg ttcgtatctg      600

gaagatcagg acatgtggcg tatgtcaggc atcttccgcg atgtgtcgct gctgcataaa      660

ccgaccacgc agatttcaga ctttcacgtt gcgacccgtt tcaatgatga cttttcgcgc      720

gccgtcctgg aagcagaagt gcagatgtgc ggtgaactgc gtgattatct gcgcgtgacg      780

gtttcactgt ggcagggtga aacccaggtg gctagcggca cggccccgtt tggcggtgaa      840

attatcgatg aacgtggcgg ttacgcggac cgtgtgaccc tgcgtctgaa cgttgaaaat      900

ccgaaactgt ggagcgcaga aattccgaac ctgtatcgcg ctgtcgtgga actgcatacc      960

gcggatggta cgctgatcga agcagaagct tgtgacgttg gcttccgtga agtccgcatt     1020

gaaaatggtc tgctgctgct gaacggcaaa ccgctgctga tccgtggtgt gaatcgccat     1080

gaacatcacc cgctgcacgg ccaggtgatg gatgaacaga cgatggtgca agacattctg     1140

ctgatgaaac agaacaattt taacgcggtt cgttgcagtc attacccgaa tcacccgctg     1200

tggtataccc tgtgtgatcg ctatggtctg tacgttgtcg acgaagcgaa catcgaaacc     1260

cacggcatgg tgccgatgaa tcgtctgacc gatgacccgc gctggctgcc ggcaatgagc     1320

gaacgtgtta cccgcatggt ccagcgtgat cgcaaccacc cgagtgttat tatctggtcc     1380

ctgggcaatg aatcaggtca tggcgccaac cacgatgcac tgtaccgttg gattaaaagc     1440

gtcgacccgt ctcgcccggt gcagtatgaa ggcggtggcg ctgataccac cgccaccgac     1500

attatctgcc cgatgtacgc tcgtgttgat gaagaccagc cgtttccggc ggtcccgaaa     1560

tggtcaatca aaaaatggct gagcctgccg ggtgaaaccc gtccgctgat cctgtgtgaa     1620

tatgcgcatg cgatgggcaa cagcctgggt ggcttcgcca aatactggca ggcatttcgt     1680

caatatccgc gcctgcaagg tggcttcgtg tgggattggg ttgaccagag tctgattaaa     1740

tacgatgaaa acggtaatcc gtggtccgcg tatggcggcg attttggcga caccccgaat     1800

gatcgtcagt tctgcatgaa cggtctggtg tttgccgacc gtaccccgca tccggcactg     1860

accgaagcaa aacaccagca acagtttttc cagttccgtc tgtctggcca aaccattgaa     1920

gtgacgagcg aatacctgtt tcgccattct gataacgaac tgctgcactg gatggttgcc     1980

ctggacggta aaccgctggc atcaggcgaa gtcccgctgg atgtggctcc gcagggtaaa     2040

caactgattg aactgccgga actgccgcag ccggaaagcg caggccaact gtggctgacc     2100

gtgcgtgtgg ttcagccgaa cgcaacggct tggagcgaag ctggtcatat ctctgcgtgg     2160

caacagtggc gcctggccga aaatctgagt gttaccctgc cggcagcttc ccatgcaatc     2220

ccgcacctga ccacgagcga aatggatttc tgtattgaac tgggcaataa acgttggcag     2280

ttcaaccgcc aaagtggttt tctgtcccag atgtggattg gcgataaaaa acaactgctg     2340

accccgctgc gtgaccagtt tacccgtgcc ccgctggata acgacatcgg tgtgtctgaa     2400

gctacccgta ttgatccgaa tgcgtgggtt gaacgctgga aagcggccgg ccattaccag     2460

gccgaagcag ctctgctgca atgtaccgca gataccctgg ccgatgcggt gctgattacc     2520

acggcacatg cttggcagca ccaaggtaaa accctgttta tcagccgtaa aacgtatcgc     2580

attgatggtt ctggccagat ggcgattacc gtcgatgtgg aagttgcaag cgacacgccg     2640

cacccggcac gtattggtct gaattgtcag ctggcacaag ttgctgaacg tgtcaactgg     2700

ctgggtctgg gtccgcagga aaattatccg gatcgtctga ccgccgcatg ctttgatcgt     2760

tgggacctgc cgctgagcga tatgtatacc ccgtacgtgt ttccgtctga aaacggtctg     2820

cgttgtggca cgcgcgaact gaattacggt ccgcatcagt ggcgtggcga ttttcaattc     2880

aacattagcc gctattctca acagcaactg atggaaacca gccatcgtca cctgctgcat     2940

gcggaagaag gtacgtggct gaatatcgat ggtttccaca tgggcattgg tggcgatgac     3000

agctggtctc cgagtgtttc cgccgaattt cagctgtctg caggccgcta tcattaccag     3060

ctggtctggt gtcaaaaagg tggcggtggc tcgggtggcg gtggcagcgg tggcggtggc     3120

tctatgaaca ttatcaatac cagtatcctg aatctgcgtt acgaatccaa ccacctgatt     3180

gatctgtcac gctatgcctc gaaaatcaac atcggttcaa aagtgaactt cgatccgatc     3240

gacaaaaatc agattcaact gtttaacctg gaaagctcta aaattgaagt catcctgaaa     3300

aacgcgattg tgtataattc catgtacgaa aacttctcaa cctcgttttg gattcgtatc     3360

ccgaaatact ttaacagcat ctctctgaac aacgaataca cgatcatcaa ctgcatggaa     3420

aacaatagtg gttggaaagt gtccctgaac tatggcgaaa ttatctggac cctgcaggat     3480

acgcaagaaa ttaaacaacg tgtcgtgttc aaatacagtc agatgatcaa catctccgat     3540

tacatcaacc gttggatctt tgttaccatt acgaacaatc gcctgaacaa cagcaaaatc     3600

tacatcaacg gtcgcctgat cgatcagaaa ccgatttcaa acctgggcaa tatccatgcg     3660

tcgaacaata ttatgttcaa actggatggc tgtcgtgaca cccaccgcta catttggatc     3720

aaatacttca acctgtttga taaagaactg aacgaaaaag aaatcaaaga tctgtatgac     3780

aaccagagta attccggtat tctgaaagat ttttggggcg actatctgca gtacgataaa     3840

ccgtattaca tgctgaatct gtacgatccg aacaaatatg tcgacgtgaa caatgtcggt     3900

attcgtggct atatgtacct gaaaggtccg cgcggcagcg tgatgaccac gaacatctac     3960

ctgaatagtt ccctgtaccg tggcaccaaa ttcatcatca aaaaatacgc ctctggcaac     4020

aaagataata tcgttcgtaa caatgaccgc gtctacatca acgttgtcgt gaaaaacaaa     4080

gaataccgcc tggcgaccaa cgccagtcag gcaggtgtcg aaaaaatcct gtccgcgctg     4140

gaaattccgg atgtgggcaa cctgtcacaa gttgtcgtga tgaaatcgaa aaatgatcag     4200

ggtattacca ataaatgcaa aatgaacctg caggataaca atggcaatga cattggtttt     4260

atcggcttcc atcagtttaa caatatcgct aaactggtgg cgagtaactg gtataatcgt     4320

cagattgaac gttcatcgcg caccctgggc tgttcctggg aatttatccc ggttgatgac     4380

ggttggggcg aacgcccgct gcatcaccat caccatcact aaggatcc                  4428


<210>  10
<211>  381
<212>  DNA
<213>  Mus musculus

<400>  10
ttccctgatg tcattaaaca tctccagtct gatgagtatg cactgctaac taggaatgtg       60

caaaaggata aatatgcaaa cttcagcatt aactttacca tggagaacca gatccacact      120

ggaatggaat acgagaacgg cagattcctc ggagtcaagt tcaaatcggt aaccttcaaa      180

gattcagtgt ttaagtcctg cacctttgac gacgtgacct cagtcaacac ctacttcaag      240

aactgcacgt ttattgatac cctttttgat aacacagatt ttgagcccta taaattcata      300

gacagtgaat ttcagaactg ctcatttctt cacaataaga cggggtgcca gattactttt      360

gatgacgact atagtgccta c                                                381


<210>  11
<211>  783
<212>  DNA
<213>  Schistosoma japonicum

<400>  11
atgtcgccga tcctgggcta ctggaaaatc aaaggcctgg tgcaaccgac ccgtctgctg       60

ctggaatacc tggaagaaaa atacgaagaa catctgtatg aacgtgatga aggcgacaaa      120

tggcgcaaca aaaaattcga actgggtctg gaatttccga atctgccgta ttacatcgat      180

ggcgacgtga aactgaccca gagcatggct attatccgtt acattgcgga taaacacaac      240

atgctgggcg gttgcccgaa agaacgcgcc gaaatctcta tgctggaagg cgcagtgctg      300

gatattcgtt atggtgttag tcgcattgcg tactccaaag atttcgaaac cctgaaagtt      360

gactttctga gcaaactgcc ggaaatgctg aaaatgttcg aagatcgtct gtgtcataaa      420

acctatctga atggtgatca tgtcacgcac ccggacttta tgctgtatga tgccctggac      480

gtggttctgt acatggaccc gatgtgcctg gacgccttcc cgaaactggt gtgttttaaa      540

aaacgcattg aagcaatccc gcaaatcgat aaatacctga aaagctctaa atacattgcg      600

tggccgctgc agggttggca agccaccttt ggcggtggcg atcacccgcc gaaaagtgac      660

ggtagtacgt ccggttcagg ctcggcgggt ctggttccgc gtggctccac cgcaatcggt      720

atgaaagaaa cggcggcggc aaaattcgaa cgccagcaca tggatagccc ggacctgggc      780

acc                                                                    783


<210>  12
<211>  261
<212>  PRT
<213>  Schistosoma japonicum

<400>  12

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser 
    210                 215                 220                 


Gly Ser Gly Ser Ala Gly Leu Val Pro Arg Gly Ser Thr Ala Ile Gly 
225                 230                 235                 240 


Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
                245                 250                 255     


Pro Asp Leu Gly Thr 
            260     


<210>  13
<211>  127
<212>  PRT
<213>  Mus musculus

<400>  13

Phe Pro Asp Val Ile Lys His Leu Gln Ser Asp Glu Tyr Ala Leu Leu 
1               5                   10                  15      


Thr Arg Asn Val Gln Lys Asp Lys Tyr Ala Asn Phe Ser Ile Asn Phe 
            20                  25                  30          


Thr Met Glu Asn Gln Ile His Thr Gly Met Glu Tyr Glu Asn Gly Arg 
        35                  40                  45              


Phe Leu Gly Val Lys Phe Lys Ser Val Thr Phe Lys Asp Ser Val Phe 
    50                  55                  60                  


Lys Ser Cys Thr Phe Asp Asp Val Thr Ser Val Asn Thr Tyr Phe Lys 
65                  70                  75                  80  


Asn Cys Thr Phe Ile Asp Thr Leu Phe Asp Asn Thr Asp Phe Glu Pro 
                85                  90                  95      


Tyr Lys Phe Ile Asp Ser Glu Phe Gln Asn Cys Ser Phe Leu His Asn 
            100                 105                 110         


Lys Thr Gly Cys Gln Ile Thr Phe Asp Asp Asp Tyr Ser Ala Tyr 
        115                 120                 125         


<210>  14
<211>  1176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fusion receptor protein GST-SV2C

<400>  14
catatgtcgc cgatcctggg ctactggaaa atcaaaggcc tggtgcaacc gacccgtctg       60

ctgctggaat acctggaaga aaaatacgaa gaacatctgt atgaacgtga tgaaggcgac      120

aaatggcgca acaaaaaatt cgaactgggt ctggaatttc cgaatctgcc gtattacatc      180

gatggcgacg tgaaactgac ccagagcatg gctattatcc gttacattgc ggataaacac      240

aacatgctgg gcggttgccc gaaagaacgc gccgaaatct ctatgctgga aggcgcagtg      300

ctggatattc gttatggtgt tagtcgcatt gcgtactcca aagatttcga aaccctgaaa      360

gttgactttc tgagcaaact gccggaaatg ctgaaaatgt tcgaagatcg tctgtgtcat      420

aaaacctatc tgaatggtga tcatgtcacg cacccggact ttatgctgta tgatgccctg      480

gacgtggttc tgtacatgga cccgatgtgc ctggacgcct tcccgaaact ggtgtgtttt      540

aaaaaacgca ttgaagcaat cccgcaaatc gataaatacc tgaaaagctc taaatacatt      600

gcgtggccgc tgcagggttg gcaagccacc tttggcggtg gcgatcaccc gccgaaaagt      660

gacggtagta cgtccggttc aggctcggcg ggtctggttc cgcgtggctc caccgcaatc      720

ggtatgaaag aaacggcggc ggcaaaattc gaacgccagc acatggatag cccggacctg      780

ggcacctttc cggatgtcat taaacacctg cagtctgacg aatatgctct gctgacgcgt      840

aacgtgcaaa aagataaata cgcgaacttt agcatcaatt tcacgatgga aaaccaaatt      900

catacgggta tggaatatga aaatggtcgc tttctgggcg ttaaattcaa atcagtcacc      960

ttcaaagata gcgttttcaa atcttgcacg tttgatgacg tcacctcggt gaacacgtac     1020

ttcaaaaact gtaccttcat cgatacgctg ttcgataaca ccgactttga accgtacaaa     1080

ttcatcgact cagaatttca gaactgctcg ttcctgcaca ataaaacggg ctgccagatt     1140

acctttgacg atgattactc cgcatactaa ggatcc                               1176


<210>  15
<211>  388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion receptor protein GST-SV2C

<400>  15

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser 
    210                 215                 220                 


Gly Ser Gly Ser Ala Gly Leu Val Pro Arg Gly Ser Thr Ala Ile Gly 
225                 230                 235                 240 


Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
                245                 250                 255     


Pro Asp Leu Gly Thr Phe Pro Asp Val Ile Lys His Leu Gln Ser Asp 
            260                 265                 270         


Glu Tyr Ala Leu Leu Thr Arg Asn Val Gln Lys Asp Lys Tyr Ala Asn 
        275                 280                 285             


Phe Ser Ile Asn Phe Thr Met Glu Asn Gln Ile His Thr Gly Met Glu 
    290                 295                 300                 


Tyr Glu Asn Gly Arg Phe Leu Gly Val Lys Phe Lys Ser Val Thr Phe 
305                 310                 315                 320 


Lys Asp Ser Val Phe Lys Ser Cys Thr Phe Asp Asp Val Thr Ser Val 
                325                 330                 335     


Asn Thr Tyr Phe Lys Asn Cys Thr Phe Ile Asp Thr Leu Phe Asp Asn 
            340                 345                 350         


Thr Asp Phe Glu Pro Tyr Lys Phe Ile Asp Ser Glu Phe Gln Asn Cys 
        355                 360                 365             


Ser Phe Leu His Asn Lys Thr Gly Cys Gln Ile Thr Phe Asp Asp Asp 
    370                 375                 380                 


Tyr Ser Ala Tyr 
385             


<210>  16
<211>  432
<212>  PRT
<213>  Clostridium botulinum

<400>  16

Ile Leu Asn Asn Ile Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu 
1               5                   10                  15      


Ile Asp Leu Ser Gly Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val 
            20                  25                  30          


Glu Leu Asn Asp Lys Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser 
        35                  40                  45              


Lys Ile Arg Val Thr Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe 
    50                  55                  60                  


Leu Asp Phe Ser Val Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn 
65                  70                  75                  80  


Asp Gly Ile Gln Asn Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Lys Asn Asn Ser Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile 
            100                 105                 110         


Ile Trp Thr Leu Ile Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe 
        115                 120                 125             


Glu Tyr Asn Ile Arg Glu Asp Ile Ser Glu Tyr Ile Asn Arg Trp Phe 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Leu Asn Asn Ala Lys Ile Tyr Ile Asn 
145                 150                 155                 160 


Gly Lys Leu Glu Ser Asn Thr Asp Ile Lys Asp Ile Arg Glu Val Ile 
                165                 170                 175     


Ala Asn Gly Glu Ile Ile Phe Lys Leu Asp Gly Asp Ile Asp Arg Thr 
            180                 185                 190         


Gln Phe Ile Trp Met Lys Tyr Phe Ser Ile Phe Asn Thr Glu Leu Ser 
        195                 200                 205             


Gln Ser Asn Ile Glu Glu Arg Tyr Lys Ile Gln Ser Tyr Ser Glu Tyr 
    210                 215                 220                 


Leu Lys Asp Phe Trp Gly Asn Pro Leu Met Tyr Asn Lys Glu Tyr Tyr 
225                 230                 235                 240 


Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp 
                245                 250                 255     


Ser Pro Val Gly Glu Ile Leu Thr Arg Ser Lys Tyr Asn Gln Asn Ser 
            260                 265                 270         


Lys Tyr Ile Asn Tyr Arg Asp Leu Tyr Ile Gly Glu Lys Phe Ile Ile 
        275                 280                 285             


Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Lys 
    290                 295                 300                 


Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg 
305                 310                 315                 320 


Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu 
                325                 330                 335     


Ala Pro Ile Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys 
            340                 345                 350         


Glu Tyr Asp Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys 
        355                 360                 365             


Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe 
    370                 375                 380                 


Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile 
385                 390                 395                 400 


Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys 
                405                 410                 415     


Leu Gly Cys Asn Trp Gln Phe Ile Pro Lys Asp Glu Gly Trp Thr Glu 
            420                 425                 430         


<210>  17
<211>  1408
<212>  DNA
<213>  Clostridium botulinum

<400>  17
attttaaata atattatctt aaatttaaga tataaggata ataatttaat agatttatca       60

ggatatgggg caaaggtaga ggtatatgat ggagtcgagc ttaatgataa aaatcaattt      120

aaattaacta gttcagcaaa tagtaagatt agagtgactc aaaatcagaa tatcatattt      180

aatagtgtgt tccttgattt tagcgttagc ttttggataa gaatacctaa atataagaat      240

gatggtatac aaaattatat tcataatgaa tatacaataa ttaattgtat gaaaaataat      300

tcgggctgga aaatatctat taggggtaat aggataatat ggactttaat tgatataaat      360

ggaaaaacca aatcggtatt ttttgaatat aacataagag aagatatatc agagtatata      420

aatagatggt tttttgtaac tattactaat aatttgaata acgctaaaat ttatattaat      480

ggtaagctag aatcaaatac agatattaaa gatataagag aagttattgc taatggtgaa      540

ataatattta aattagatgg tgatatagat agaacacaat ttatttggat gaaatatttc      600

agtattttta atacggaatt aagtcaatca aatattgaag aaagatataa aattcaatca      660

tatagcgaat atttaaaaga tttttgggga aatcctttaa tgtacaataa agaatattat      720

atgtttaatg cggggaataa aaattcatat attaaactaa agaaagattc acctgtaggt      780

gaaattttaa cacgtagcaa atataatcaa aattctaaat atataaatta tagagattta      840

tatattggag aaaaatttat tataagaaga aagtcaaatt ctcaatctat aaatgatgat      900

atagttagaa aagaagatta tatatatcta gattttttta atttaaatca agagtggaga      960

gtatatacct ataaatattt taagaaagag gaagaaaaat tgtttttagc tcctataagt     1020

gattctgatg agttttacaa tactatacaa ataaaagaat atgatgaaca gccaacatat     1080

agttgtcagt tgctttttaa aaaagatgaa gaaagtactg atgagatagg attgattggt     1140

attcatcgtt tctacgaatc tggaattgta tttgaagagt ataaagatta tttttgtata     1200

agtaaatggt acttaaaaga ggtaaaaagg aaaccatata atttaaaatt gggatgtaat     1260

tggcagttta ttcctaaaga tgaagggtgg actgaataat ataactatat gctcagcaaa     1320

cctattttat ataagaaaag tttaagttta taaaatctta agtttaagga tgtagctaaa     1380

ttttgaatat tagataaact acatgttt                                        1408


<210>  18
<211>  449
<212>  PRT
<213>  Clostridium botulinum

<400>  18

Thr Ile Pro Phe Asn Ile Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys 
1               5                   10                  15      


Asp Ile Ile Asn Glu Tyr Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu 
            20                  25                  30          


Ser Leu Gln Asn Arg Lys Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn 
        35                  40                  45              


Ala Glu Val Ser Glu Glu Gly Asp Val Gln Leu Asn Pro Ile Phe Pro 
    50                  55                  60                  


Phe Asp Phe Lys Leu Gly Ser Ser Gly Glu Asp Arg Gly Lys Val Ile 
65                  70                  75                  80  


Val Thr Gln Asn Glu Asn Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe 
                85                  90                  95      


Ser Ile Ser Phe Trp Ile Arg Ile Asn Lys Trp Val Ser Asn Leu Pro 
            100                 105                 110         


Gly Tyr Thr Ile Ile Asp Ser Val Lys Asn Asn Ser Gly Trp Ser Ile 
        115                 120                 125             


Gly Ile Ile Ser Asn Phe Leu Val Phe Thr Leu Lys Gln Asn Glu Asp 
    130                 135                 140                 


Ser Glu Gln Ser Ile Asn Phe Ser Tyr Asp Ile Ser Asn Asn Ala Pro 
145                 150                 155                 160 


Gly Tyr Asn Lys Trp Phe Phe Val Thr Val Thr Asn Asn Met Met Gly 
                165                 170                 175     


Asn Met Lys Ile Tyr Ile Asn Gly Lys Leu Ile Asp Thr Ile Lys Val 
            180                 185                 190         


Lys Glu Leu Thr Gly Ile Asn Phe Ser Lys Thr Ile Thr Phe Glu Ile 
        195                 200                 205             


Asn Lys Ile Pro Asp Thr Gly Leu Ile Thr Ser Asp Ser Asp Asn Ile 
    210                 215                 220                 


Asn Met Trp Ile Arg Asp Phe Tyr Ile Phe Ala Lys Glu Leu Asp Gly 
225                 230                 235                 240 


Lys Asp Ile Asn Ile Leu Phe Asn Ser Leu Gln Tyr Thr Asn Val Val 
                245                 250                 255     


Lys Asp Tyr Trp Gly Asn Asp Leu Arg Tyr Asn Lys Glu Tyr Tyr Met 
            260                 265                 270         


Val Asn Ile Asp Tyr Leu Asn Arg Tyr Met Tyr Ala Asn Ser Arg Gln 
        275                 280                 285             


Ile Val Phe Asn Thr Arg Arg Asn Asn Asn Asp Phe Asn Glu Gly Tyr 
    290                 295                 300                 


Lys Ile Ile Ile Lys Arg Ile Arg Gly Asn Thr Asn Asp Thr Arg Val 
305                 310                 315                 320 


Arg Gly Gly Asp Ile Leu Tyr Phe Asp Met Thr Ile Asn Asn Lys Ala 
                325                 330                 335     


Tyr Asn Leu Phe Met Lys Asn Glu Thr Met Tyr Ala Asp Asn His Ser 
            340                 345                 350         


Thr Glu Asp Ile Tyr Ala Ile Gly Leu Arg Glu Gln Thr Lys Asp Ile 
        355                 360                 365             


Asn Asp Asn Ile Ile Phe Gln Ile Gln Pro Met Asn Asn Thr Tyr Tyr 
    370                 375                 380                 


Tyr Ala Ser Gln Ile Phe Lys Ser Asn Phe Asn Gly Glu Asn Ile Ser 
385                 390                 395                 400 


Gly Ile Cys Ser Ile Gly Thr Tyr Arg Phe Arg Leu Gly Gly Asp Trp 
                405                 410                 415     


Tyr Arg His Asn Tyr Leu Val Pro Thr Val Lys Gln Gly Asn Tyr Ala 
            420                 425                 430         


Ser Leu Leu Glu Ser Thr Ser Thr His Trp Gly Phe Val Pro Val Ser 
        435                 440                 445             


Glu 
    


<210>  19
<211>  1347
<212>  DNA
<213>  Clostridium botulinum

<400>  19
acaataccct ttaatatttt ttcatatact aataattctt tattaaaaga tataattaat       60

gaatatttca ataatattaa tgattcaaaa attttgagcc tacaaaacag aaaaaatact      120

ttagtggata catcaggata taatgcagaa gtgagtgaag aaggcgatgt tcagcttaat      180

ccaatatttc catttgactt taaattaggt agttcagggg aggatagagg taaagttata      240

gtaacccaga atgaaaatat tgtatataat tctatgtatg aaagttttag cattagtttt      300

tggattagaa taaataaatg ggtaagtaat ttacctggat atactataat tgatagtgtt      360

aaaaataact caggttggag tataggtatt attagtaatt ttttagtatt tactttaaaa      420

caaaatgaag atagtgaaca aagtataaat tttagttatg atatatcaaa taatgctcct      480

ggatacaata aatggttttt tgtaactgtt actaacaata tgatgggaaa tatgaagatt      540

tatataaatg gaaaattaat agatactata aaagttaaag aactaactgg aattaatttt      600

agcaaaacta taacatttga aataaataaa attccagata ccggtttgat tacttcagat      660

tctgataaca tcaatatgtg gataagagat ttttatatat ttgctaaaga attagatggt      720

aaagatatta atatattatt taatagcttg caatatacta atgttgtaaa agattattgg      780

ggaaatgatt taagatataa taaagaatat tatatggtta atatagatta tttaaataga      840

tatatgtatg cgaactcacg acaaattgtt tttaatacac gtagaaataa taatgacttc      900

aatgaaggat ataaaattat aataaaaaga atcagaggaa atacaaatga tactagagta      960

cgaggaggag atattttata ttttgatatg acaattaata acaaagcata taatttgttt     1020

atgaagaatg aaactatgta tgcagataat catagtactg aagatatata tgctataggt     1080

ttaagagaac aaacaaagga tataaatgat aatattatat ttcaaataca accaatgaat     1140

aatacttatt attacgcatc tcaaatattt aaatcaaatt ttaatggaga aaatatttct     1200

ggaatatgtt caataggtac ttatcgtttt agacttggag gtgattggta tagacacaat     1260

tatttggtgc ctactgtgaa gcaaggaaat tatgcttcat tattagaatc aacatcaact     1320

cattggggtt ttgtacctgt aagtgaa                                         1347


<210>  20
<211>  416
<212>  PRT
<213>  Clostridium botulinum

<400>  20

Phe Asn Ser Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys 
1               5                   10                  15      


Asn Ala Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly 
            20                  25                  30          


Asp Asn Val Gln Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser 
        35                  40                  45              


Ser Ser Gly Asp Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr 
    50                  55                  60                  


Ser Ala Ile Tyr Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser 
65                  70                  75                  80  


Lys Asp Leu Thr Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile 
                85                  90                  95      


Glu Gln Asn Ser Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu 
            100                 105                 110         


Trp Ile Leu Gln Asp Val Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp 
        115                 120                 125             


Tyr Ser Glu Ser Leu Ser His Thr Gly Tyr Thr Asn Lys Trp Phe Phe 
    130                 135                 140                 


Val Thr Ile Thr Asn Asn Ile Met Gly Tyr Met Lys Leu Tyr Ile Asn 
145                 150                 155                 160 


Gly Glu Leu Lys Gln Ser Gln Lys Ile Glu Asp Leu Asp Glu Val Lys 
                165                 170                 175     


Leu Asp Lys Thr Ile Val Phe Gly Ile Asp Glu Asn Ile Asp Glu Asn 
            180                 185                 190         


Gln Met Leu Trp Ile Arg Asp Phe Asn Ile Phe Ser Lys Glu Leu Ser 
        195                 200                 205             


Asn Glu Asp Ile Asn Ile Val Tyr Glu Gly Gln Ile Leu Arg Asn Val 
    210                 215                 220                 


Ile Lys Asp Tyr Trp Gly Asn Pro Leu Lys Phe Asp Thr Glu Tyr Tyr 
225                 230                 235                 240 


Ile Ile Asn Asp Asn Tyr Ile Asp Arg Tyr Ile Ala Pro Glu Ser Asn 
                245                 250                 255     


Val Leu Val Leu Val Gln Tyr Pro Asp Arg Ser Lys Leu Tyr Thr Gly 
            260                 265                 270         


Asn Pro Ile Thr Ile Lys Ser Val Ser Asp Lys Asn Pro Tyr Ser Arg 
        275                 280                 285             


Ile Leu Asn Gly Asp Asn Ile Ile Leu His Met Leu Tyr Asn Ser Arg 
    290                 295                 300                 


Lys Tyr Met Ile Ile Arg Asp Thr Asp Thr Ile Tyr Ala Thr Gln Gly 
305                 310                 315                 320 


Gly Glu Cys Ser Gln Asn Cys Val Tyr Ala Leu Lys Leu Gln Ser Asn 
                325                 330                 335     


Leu Gly Asn Tyr Gly Ile Gly Ile Phe Ser Ile Lys Asn Ile Val Ser 
            340                 345                 350         


Lys Asn Lys Tyr Cys Ser Gln Ile Phe Ser Ser Phe Arg Glu Asn Thr 
        355                 360                 365             


Met Leu Leu Ala Asp Ile Tyr Lys Pro Trp Arg Phe Ser Phe Lys Asn 
    370                 375                 380                 


Ala Tyr Thr Pro Val Ala Val Thr Asn Tyr Glu Thr Lys Leu Leu Ser 
385                 390                 395                 400 


Thr Ser Ser Phe Trp Lys Phe Ile Ser Arg Asp Pro Gly Trp Val Glu 
                405                 410                 415     


<210>  21
<211>  1248
<212>  DNA
<213>  Clostridium botulinum

<400>  21
ttcaatagta ttaatgattc aaaaattttg agcttacaaa acaaaaaaaa tgctttagtg       60

gatacatcag gatataatgc agaagtgagg gtaggagata atgttcaact taatacgata      120

tatacaaatg actttaaatt aagtagttca ggagataaaa ttatagtaaa tttaaataat      180

aatattttat atagcgctat ttatgagaac tctagtgtta gtttttggat taagatatct      240

aaagatttaa ctaattctca taatgaatat acaataatta acagtataga acaaaattct      300

gggtggaaat tatgtattag gaatggcaat atagaatgga ttttacaaga tgttaataga      360

aagtataaaa gtttaatttt tgattatagt gaatcattaa gtcatacagg atatacaaat      420

aaatggtttt ttgttactat aactaataat ataatggggt atatgaaact ttatataaat      480

ggagaattaa agcagagtca aaaaattgaa gatttagatg aggttaagtt agataaaacc      540

atagtatttg gaatagatga gaatatagat gagaatcaga tgctttggat tagagatttt      600

aatatttttt ctaaagaatt aagtaatgaa gatattaata ttgtatatga gggacaaata      660

ttaagaaatg ttattaaaga ttattgggga aatcctttga agtttgatac agaatattat      720

attattaatg ataattatat agataggtat atagcacctg aaagtaatgt acttgtactt      780

gttcagtatc cagatagatc taaattatat actggaaatc ctattactat taaatcagta      840

tctgataaga atccttatag tagaatttta aatggagata atataattct tcatatgtta      900

tataatagta ggaaatatat gataataaga gatactgata caatatatgc aacacaagga      960

ggagagtgtt cacaaaattg tgtatatgca ttaaaattac agagtaattt aggtaattat     1020

ggtataggta tatttagtat aaaaaatatt gtatctaaaa ataaatattg tagtcaaatt     1080

ttctctagtt ttagggaaaa tacaatgctt ctagcagata tatataaacc ttggagattt     1140

tcttttaaaa atgcatacac gccagttgca gtaactaatt atgaaacaaa actattatca     1200

acttcatctt tttggaaatt tatttctagg gatccaggat gggtagag                  1248


<210>  22
<211>  408
<212>  PRT
<213>  Clostridium botulinum

<400>  22

Lys Arg Ile Lys Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp 
1               5                   10                  15      


Lys Tyr Val Asp Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly 
            20                  25                  30          


Asp Val Tyr Lys Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn 
        35                  40                  45              


Asp Lys Leu Ser Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr 
    50                  55                  60                  


Asp Asn Lys Tyr Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro 
65                  70                  75                  80  


Asn Tyr Asp Asn Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile 
                85                  90                  95      


Asn Cys Met Arg Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His 
            100                 105                 110         


Asn Glu Ile Ile Trp Thr Leu Gln Asp Asn Ala Gly Ile Asn Gln Lys 
        115                 120                 125             


Leu Ala Phe Asn Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn 
    130                 135                 140                 


Lys Trp Ile Phe Val Thr Ile Thr Asn Asp Arg Leu Gly Asp Ser Lys 
145                 150                 155                 160 


Leu Tyr Ile Asn Gly Asn Leu Ile Asp Gln Lys Ser Ile Leu Asn Leu 
                165                 170                 175     


Gly Asn Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Asn Cys 
            180                 185                 190         


Ser Tyr Thr Arg Tyr Ile Gly Ile Arg Tyr Phe Asn Ile Phe Asp Lys 
        195                 200                 205             


Glu Leu Asp Glu Thr Glu Ile Gln Thr Leu Tyr Ser Asn Glu Pro Asn 
    210                 215                 220                 


Thr Asn Ile Leu Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asp Lys 
225                 230                 235                 240 


Glu Tyr Tyr Leu Leu Asn Val Leu Lys Pro Asn Asn Phe Ile Asp Arg 
                245                 250                 255     


Arg Lys Asp Ser Thr Leu Ser Ile Asn Asn Ile Arg Ser Thr Ile Leu 
            260                 265                 270         


Leu Ala Asn Arg Leu Tyr Ser Gly Ile Lys Val Lys Ile Gln Arg Val 
        275                 280                 285             


Asn Asn Ser Ser Thr Asn Asp Asn Leu Val Arg Lys Asn Asp Gln Val 
    290                 295                 300                 


Tyr Ile Asn Phe Val Ala Ser Lys Thr His Leu Phe Pro Leu Tyr Ala 
305                 310                 315                 320 


Asp Thr Ala Thr Thr Asn Lys Glu Lys Thr Ile Lys Ile Ser Ser Ser 
                325                 330                 335     


Gly Asn Arg Phe Asn Gln Val Val Val Met Asn Ser Val Gly Asn Asn 
            340                 345                 350         


Cys Thr Met Asn Phe Lys Asn Asn Asn Gly Asn Asn Ile Gly Leu Leu 
        355                 360                 365             


Gly Phe Lys Ala Asp Thr Val Val Ala Ser Thr Trp Tyr Tyr Thr His 
    370                 375                 380                 


Met Arg Asp His Thr Asn Ser Asn Gly Cys Phe Trp Asn Phe Ile Ser 
385                 390                 395                 400 


Glu Glu His Gly Trp Gln Glu Lys 
                405             


<210>  23
<211>  1271
<212>  DNA
<213>  Clostridium botulinum

<400>  23
aagagaatta aaagtagttc agttttaaat atgagatata aaaatgataa atacgtagat       60

acttcaggat atgattcaaa tataaatatt aatggagatg tatataaata tccaactaat      120

aaaaatcaat ttggaatata taatgataaa cttagtgaag ttaatatatc tcaaaatgat      180

tacattatat atgataataa atataaaaat tttagtatta gtttttgggt aagaattcct      240

aactatgata ataagatagt aaatgttaat aatgaataca ctataataaa ttgtatgaga      300

gataataatt caggatggaa agtatctctt aatcataatg aaataatttg gacattgcaa      360

gataatgcag gaattaatca aaaattagca tttaactatg gtaacgcaaa tggtatttct      420

gattatataa ataagtggat ttttgtaact ataactaatg atagattagg agattctaaa      480

ctttatatta atggaaattt aatagatcaa aaatcaattt taaatttagg taatattcat      540

gttagtgaca atatattatt taaaatagtt aattgtagtt atacaagata tattggtatt      600

agatatttta atatttttga taaagaatta gatgaaacag aaattcaaac tttatatagc      660

aatgaaccta atacaaatat tttgaaggat ttttggggaa attatttgct ttatgacaaa      720

gaatactatt tattaaatgt gttaaaacca aataacttta ttgataggag aaaagattct      780

actttaagca ttaataatat aagaagcact attcttttag ctaatagatt atatagtgga      840

ataaaagtta aaatacaaag agttaataat agtagtacta acgataatct tgttagaaag      900

aatgatcagg tatatattaa ttttgtagcc agcaaaactc acttatttcc attatatgct      960

gatacagcta ccacaaataa agagaaaaca ataaaaatat catcatctgg caatagattt     1020

aatcaagtag tagttatgaa ttcagtagga aataattgta caatgaattt taaaaataat     1080

aatggaaata atattgggtt gttaggtttc aaggcagata ctgtagttgc tagtacttgg     1140

tattatacac atatgagaga tcatacaaac agcaatggat gtttttggaa ctttatttct     1200

gaagaacatg gatggcaaga aaaataaaaa ttagattaaa cggctaaagt cataaattcc     1260

aaaggactta g                                                          1271


<210>  24
<211>  419
<212>  PRT
<213>  Clostridium botulinum

<400>  24

Asn Lys Leu Tyr Lys Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg 
1               5                   10                  15      


Tyr Glu Asn Asn Lys Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile 
            20                  25                  30          


Ser Ile Asn Gly Asp Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe 
        35                  40                  45              


Gly Ile Tyr Ser Ser Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn 
    50                  55                  60                  


Asp Ile Ile Tyr Asn Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp 
65                  70                  75                  80  


Val Arg Ile Pro Lys Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr 
                85                  90                  95      


Thr Ile Ile Asp Cys Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser 
            100                 105                 110         


Leu Asn Tyr Asn Lys Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn 
        115                 120                 125             


Asn Gln Lys Leu Val Phe Asn Tyr Thr Gln Met Ile Ser Ile Ser Asp 
    130                 135                 140                 


Tyr Ile Asn Lys Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Gly 
145                 150                 155                 160 


Asn Ser Arg Ile Tyr Ile Asn Gly Asn Leu Ile Asp Glu Lys Ser Ile 
                165                 170                 175     


Ser Asn Leu Gly Asp Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile 
            180                 185                 190         


Val Gly Cys Asn Asp Thr Arg Tyr Val Gly Ile Arg Tyr Phe Lys Val 
        195                 200                 205             


Phe Asp Thr Glu Leu Gly Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asp 
    210                 215                 220                 


Glu Pro Asp Pro Ser Ile Leu Lys Asp Phe Trp Gly Asn Tyr Leu Leu 
225                 230                 235                 240 


Tyr Asn Lys Arg Tyr Tyr Leu Leu Asn Leu Leu Arg Thr Asp Lys Ser 
                245                 250                 255     


Ile Thr Gln Asn Ser Asn Phe Leu Asn Ile Asn Gln Gln Arg Gly Val 
            260                 265                 270         


Tyr Gln Lys Pro Asn Ile Phe Ser Asn Thr Arg Leu Tyr Thr Gly Val 
        275                 280                 285             


Glu Val Ile Ile Arg Lys Asn Gly Ser Thr Asp Ile Ser Asn Thr Asp 
    290                 295                 300                 


Asn Phe Val Arg Lys Asn Asp Leu Ala Tyr Ile Asn Val Val Asp Arg 
305                 310                 315                 320 


Asp Val Glu Tyr Arg Leu Tyr Ala Asp Ile Ser Ile Ala Lys Pro Glu 
                325                 330                 335     


Lys Ile Ile Lys Leu Ile Arg Thr Ser Asn Ser Asn Asn Ser Leu Gly 
            340                 345                 350         


Gln Ile Ile Val Met Asp Ser Ile Gly Asn Asn Cys Thr Met Asn Phe 
        355                 360                 365             


Gln Asn Asn Asn Gly Gly Asn Ile Gly Leu Leu Gly Phe His Ser Asn 
    370                 375                 380                 


Asn Leu Val Ala Ser Ser Trp Tyr Tyr Asn Asn Ile Arg Lys Asn Thr 
385                 390                 395                 400 


Ser Ser Asn Gly Cys Phe Trp Ser Phe Ile Ser Lys Glu His Gly Trp 
                405                 410                 415     


Gln Glu Asn 
            


<210>  25
<211>  1257
<212>  DNA
<213>  Clostridium botulinum

<400>  25
aataaattat ataaaaaaat taaagataac tctattttag atatgcgata tgaaaataat       60

aaatttatag atatctctgg atatggttca aatataagca ttaatggaga tgtatatatt      120

tattcaacaa atagaaatca atttggaata tatagtagta agcctagtga agttaatata      180

gctcaaaata atgatattat atacaatggt agatatcaaa attttagtat tagtttctgg      240

gtaaggattc ctaaatactt caataaagtg aatcttaata atgaatatac tataatagat      300

tgtataagga ataataattc aggatggaaa atatcactta attataataa aataatttgg      360

actttacaag atactgctgg aaataatcaa aaactagttt ttaattatac acaaatgatt      420

agtatatctg attatataaa taaatggatt tttgtaacta ttactaataa tagattaggc      480

aattctagaa tttacatcaa tggaaattta atagatgaaa aatcaatttc gaatttaggt      540

gatattcatg ttagtgataa tatattattt aaaattgttg gttgtaatga tacaagatat      600

gttggtataa gatattttaa agtttttgat acggaattag gtaaaacaga aattgagact      660

ttatatagtg atgagccaga tccaagtatc ttaaaagact tttggggaaa ttatttgtta      720

tataataaaa gatattattt attgaattta ctaagaacag ataagtctat tactcagaat      780

tcaaactttc taaatattaa tcaacaaaga ggtgtttatc agaaaccaaa tattttttcc      840

aacactagat tatatacagg agtagaagtt attataagaa aaaatggatc tacagatata      900

tctaatacag ataattttgt tagaaaaaat gatctggcat atattaatgt agtagatcgt      960

gatgtagaat atcggctata tgctgatata tcaattgcaa aaccagagaa aataataaaa     1020

ttaataagaa catctaattc aaacaatagc ttaggtcaaa ttatagttat ggattcaata     1080

ggaaataatt gcacaatgaa ttttcaaaac aataatgggg gcaatatagg attactaggt     1140

tttcattcaa ataatttggt tgctagtagt tggtattata acaatatacg aaaaaatact     1200

agcagtaatg gatgcttttg gagttttatt tctaaagagc atggatggca agaaaac        1257


<210>  26
<211>  438
<212>  PRT
<213>  Clostridium botulinum

<400>  26

Asn Tyr Ile Ser Asn Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr 
1               5                   10                  15      


Arg Gly Gly Arg Leu Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn 
            20                  25                  30          


Val Gly Ser Asp Val Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys 
        35                  40                  45              


Leu Asn Asn Ser Glu Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe 
    50                  55                  60                  


Val Val Tyr Asp Ser Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val 
65                  70                  75                  80  


Arg Thr Pro Lys Tyr Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn 
                85                  90                  95      


Glu Tyr Thr Ile Ile Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val 
            100                 105                 110         


Ser Ile Lys Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala 
        115                 120                 125             


Lys Ser Lys Ser Ile Phe Phe Glu Tyr Ser Ile Lys Asp Asn Ile Ser 
    130                 135                 140                 


Asp Tyr Ile Asn Lys Trp Phe Ser Ile Thr Ile Thr Asn Asp Arg Leu 
145                 150                 155                 160 


Gly Asn Ala Asn Ile Tyr Ile Asn Gly Ser Leu Lys Lys Ser Glu Lys 
                165                 170                 175     


Ile Leu Asn Leu Asp Arg Ile Asn Ser Ser Asn Asp Ile Asp Phe Lys 
            180                 185                 190         


Leu Ile Asn Cys Thr Asp Thr Thr Lys Phe Val Trp Ile Lys Asp Phe 
        195                 200                 205             


Asn Ile Phe Gly Arg Glu Leu Asn Ala Thr Glu Val Ser Ser Leu Tyr 
    210                 215                 220                 


Trp Ile Gln Ser Ser Thr Asn Thr Leu Lys Asp Phe Trp Gly Asn Pro 
225                 230                 235                 240 


Leu Arg Tyr Asp Thr Gln Tyr Tyr Leu Phe Asn Gln Gly Met Gln Asn 
                245                 250                 255     


Ile Tyr Ile Lys Tyr Phe Ser Lys Ala Ser Met Gly Glu Thr Ala Pro 
            260                 265                 270         


Arg Thr Asn Phe Asn Asn Ala Ala Ile Asn Tyr Gln Asn Leu Tyr Leu 
        275                 280                 285             


Gly Leu Arg Phe Ile Ile Lys Lys Ala Ser Asn Ser Arg Asn Ile Asn 
    290                 295                 300                 


Asn Asp Asn Ile Val Arg Glu Gly Asp Tyr Ile Tyr Leu Asn Ile Asp 
305                 310                 315                 320 


Asn Ile Ser Asp Glu Ser Tyr Arg Val Tyr Val Leu Val Asn Ser Lys 
                325                 330                 335     


Glu Ile Gln Thr Gln Leu Phe Leu Ala Pro Ile Asn Asp Asp Pro Thr 
            340                 345                 350         


Phe Tyr Asp Val Leu Gln Ile Lys Lys Tyr Tyr Glu Lys Thr Thr Tyr 
        355                 360                 365             


Asn Cys Gln Ile Leu Cys Glu Lys Asp Thr Lys Thr Phe Gly Leu Phe 
    370                 375                 380                 


Gly Ile Gly Lys Phe Val Lys Asp Tyr Gly Tyr Val Trp Asp Thr Tyr 
385                 390                 395                 400 


Asp Asn Tyr Phe Cys Ile Ser Gln Trp Tyr Leu Arg Arg Ile Ser Glu 
                405                 410                 415     


Asn Ile Asn Lys Leu Arg Leu Gly Cys Asn Trp Gln Phe Ile Pro Val 
            420                 425                 430         


Asp Glu Gly Trp Thr Glu 
        435             


<210>  27
<211>  1314
<212>  DNA
<213>  Clostridium botulinum

<400>  27
aattatatta gtaatattag tagtaatgct attttaagtt taagttatag aggtgggcgt       60

ttaatagatt catctggata tggtgcaact atgaatgtag gttcagatgt tatctttaat      120

gatataggaa atggtcaatt taaattaaat aattctgaaa atagtaatat tacggcacat      180

caaagtaaat tcgttgtata tgatagtatg tttgataatt ttagcattaa cttttgggta      240

aggactccta aatataataa taatgatata caaacttatc ttcaaaatga gtatacaata      300

attagttgta taaaaaatga ctcaggatgg aaagtatcta ttaagggaaa tagaataata      360

tggacattaa tagatgttaa tgcaaaatct aaatcaatat ttttcgaata tagtataaaa      420

gataatatat cagattatat aaataaatgg ttttccataa ctattactaa tgatagatta      480

ggtaacgcaa atatttatat aaatggaagt ttgaaaaaaa gtgaaaaaat tttaaactta      540

gatagaatta attctagtaa tgatatagac ttcaaattaa ttaattgtac agatactact      600

aaatttgttt ggattaagga ttttaatatt tttggtagag aattaaatgc tacagaagta      660

tcttcactat attggattca atcatctaca aatactttaa aagatttttg ggggaatcct      720

ttaagatacg atacacaata ctatctgttt aatcaaggta tgcaaaatat ctatataaag      780

tattttagta aagcttctat gggggaaact gcaccacgta caaactttaa taatgcagca      840

ataaattatc aaaatttata tcttggttta cgatttatta taaaaaaagc atcaaattct      900

cggaatataa ataatgataa tatagtcaga gaaggagatt atatatatct taatattgat      960

aatatttctg atgaatctta cagagtatat gttttggtga attctaaaga aattcaaact     1020

caattatttt tagcacccat aaatgatgat cctacgttct atgatgtact acaaataaaa     1080

aaatattatg aaaaaacaac atataattgt cagatacttt gcgaaaaaga tactaaaaca     1140

tttgggctgt ttggaattgg taaatttgtt aaagattatg gatatgtttg ggatacctat     1200

gataattatt tttgcataag tcagtggtat ctcagaagaa tatctgaaaa tataaataaa     1260

ttaaggttgg gatgtaattg gcaattcatt cccgtggatg aaggatggac agaa           1314


<210>  28
<211>  742
<212>  PRT
<213>  Mus musculus

<400>  28

Met Glu Glu Gly Phe Arg Asp Arg Ala Ala Phe Ile Arg Gly Ala Lys 
1               5                   10                  15      


Asp Ile Ala Lys Glu Val Lys Lys His Ala Ala Lys Lys Val Val Lys 
            20                  25                  30          


Gly Leu Asp Arg Val Gln Asp Glu Tyr Ser Arg Arg Ser Tyr Ser Arg 
        35                  40                  45              


Phe Glu Glu Glu Asp Asp Asp Asp Asp Phe Pro Ala Pro Ala Asp Gly 
    50                  55                  60                  


Tyr Tyr Arg Gly Glu Gly Ala Gln Asp Glu Glu Glu Gly Gly Ala Ser 
65                  70                  75                  80  


Ser Asp Ala Thr Glu Gly His Asp Glu Asp Asp Glu Ile Tyr Glu Gly 
                85                  90                  95      


Glu Tyr Gln Gly Ile Pro Arg Ala Glu Ser Gly Gly Lys Gly Glu Arg 
            100                 105                 110         


Met Ala Asp Gly Ala Pro Leu Ala Gly Val Arg Gly Gly Leu Ser Asp 
        115                 120                 125             


Gly Glu Gly Pro Pro Gly Gly Arg Gly Glu Ala Gln Arg Arg Lys Asp 
    130                 135                 140                 


Arg Glu Glu Leu Ala Gln Gln Tyr Glu Thr Ile Leu Arg Glu Cys Gly 
145                 150                 155                 160 


His Gly Arg Phe Gln Trp Thr Leu Tyr Phe Val Leu Gly Leu Ala Leu 
                165                 170                 175     


Met Ala Asp Gly Val Glu Val Phe Val Val Gly Phe Val Leu Pro Ser 
            180                 185                 190         


Ala Glu Lys Asp Met Cys Leu Ser Asp Ser Asn Lys Gly Met Leu Gly 
        195                 200                 205             


Leu Ile Val Tyr Leu Gly Met Met Val Gly Ala Phe Leu Trp Gly Gly 
    210                 215                 220                 


Leu Ala Asp Arg Leu Gly Arg Arg Gln Cys Leu Leu Ile Ser Leu Ser 
225                 230                 235                 240 


Val Asn Ser Val Phe Ala Phe Phe Ser Ser Phe Val Gln Gly Tyr Gly 
                245                 250                 255     


Thr Phe Leu Phe Cys Arg Leu Leu Ser Gly Val Gly Ile Gly Gly Ser 
            260                 265                 270         


Ile Pro Ile Val Phe Ser Tyr Phe Ser Glu Phe Leu Ala Gln Glu Lys 
        275                 280                 285             


Arg Gly Glu His Leu Ser Trp Leu Cys Met Phe Trp Met Ile Gly Gly 
    290                 295                 300                 


Val Tyr Ala Ala Ala Met Ala Trp Ala Ile Ile Pro His Tyr Gly Trp 
305                 310                 315                 320 


Ser Phe Gln Met Gly Ser Ala Tyr Gln Phe His Ser Trp Arg Val Phe 
                325                 330                 335     


Val Leu Val Cys Ala Phe Pro Ser Val Phe Ala Ile Gly Ala Leu Thr 
            340                 345                 350         


Thr Gln Pro Glu Ser Pro Arg Phe Phe Leu Glu Asn Gly Lys His Asp 
        355                 360                 365             


Glu Ala Trp Met Val Leu Lys Gln Val His Asp Thr Asn Met Arg Ala 
    370                 375                 380                 


Lys Gly His Pro Glu Arg Val Phe Ser Val Thr His Ile Lys Thr Ile 
385                 390                 395                 400 


His Gln Glu Asp Glu Leu Ile Glu Ile Gln Ser Asp Thr Gly Thr Trp 
                405                 410                 415     


Tyr Gln Arg Trp Gly Val Arg Ala Leu Ser Leu Gly Gly Gln Val Trp 
            420                 425                 430         


Gly Asn Phe Leu Ser Cys Phe Ser Pro Glu Tyr Arg Arg Ile Thr Leu 
        435                 440                 445             


Met Met Met Gly Val Trp Phe Thr Met Ser Phe Ser Tyr Tyr Gly Leu 
    450                 455                 460                 


Thr Val Trp Phe Pro Asp Met Ile Arg His Leu Gln Ala Val Asp Tyr 
465                 470                 475                 480 


Ala Ala Arg Thr Lys Val Phe Pro Gly Glu Arg Val Glu His Val Thr 
                485                 490                 495     


Phe Asn Phe Thr Leu Glu Asn Gln Ile His Arg Gly Gly Gln Tyr Phe 
            500                 505                 510         


Asn Asp Lys Phe Ile Gly Leu Arg Leu Lys Ser Val Ser Phe Glu Asp 
        515                 520                 525             


Ser Leu Phe Glu Glu Cys Tyr Phe Glu Asp Val Thr Ser Ser Asn Thr 
    530                 535                 540                 


Phe Phe Arg Asn Cys Thr Phe Ile Asn Thr Val Phe Tyr Asn Thr Asp 
545                 550                 555                 560 


Leu Phe Glu Tyr Lys Phe Val Asn Ser Arg Leu Val Asn Ser Thr Phe 
                565                 570                 575     


Leu His Asn Lys Glu Gly Cys Pro Leu Asp Val Thr Gly Thr Gly Glu 
            580                 585                 590         


Gly Ala Tyr Met Val Tyr Phe Val Ser Phe Leu Gly Thr Leu Ala Val 
        595                 600                 605             


Leu Pro Gly Asn Ile Val Ser Ala Leu Leu Met Asp Lys Ile Gly Arg 
    610                 615                 620                 


Leu Arg Met Leu Ala Gly Ser Ser Val Leu Ser Cys Val Ser Cys Phe 
625                 630                 635                 640 


Phe Leu Ser Phe Gly Asn Ser Glu Ser Ala Met Ile Ala Leu Leu Cys 
                645                 650                 655     


Leu Phe Gly Gly Val Ser Ile Ala Ser Trp Asn Ala Leu Asp Val Leu 
            660                 665                 670         


Thr Val Glu Leu Tyr Pro Ser Asp Lys Arg Thr Thr Ala Phe Gly Phe 
        675                 680                 685             


Leu Asn Ala Leu Cys Lys Leu Ala Ala Val Leu Gly Ile Ser Ile Phe 
    690                 695                 700                 


Thr Ser Phe Val Gly Ile Thr Lys Ala Ala Pro Ile Leu Phe Ala Ser 
705                 710                 715                 720 


Ala Ala Leu Ala Leu Gly Ser Ser Leu Ala Leu Lys Leu Pro Glu Thr 
                725                 730                 735     


Arg Gly Gln Val Leu Gln 
            740         


<210>  29
<211>  683
<212>  PRT
<213>  Mus musculus

<400>  29

Met Asp Asp Tyr Arg Tyr Arg Asp Asn Tyr Glu Gly Tyr Ala Pro Ser 
1               5                   10                  15      


Asp Gly Tyr Tyr Arg Ser Asn Glu Gln Asn Gln Glu Glu Asp Ala Gln 
            20                  25                  30          


Ser Asp Val Thr Glu Gly His Asp Glu Glu Asp Glu Ile Tyr Glu Gly 
        35                  40                  45              


Glu Tyr Gln Gly Ile Pro His Pro Asp Asp Val Lys Ser Lys Gln Thr 
    50                  55                  60                  


Lys Met Ala Pro Ser Arg Ala Asp Gly Leu Gly Gly Gln Ala Asp Leu 
65                  70                  75                  80  


Met Ala Glu Arg Met Glu Asp Glu Glu Glu Leu Ala His Gln Tyr Glu 
                85                  90                  95      


Thr Ile Ile Asp Glu Cys Gly His Gly Arg Phe Gln Trp Thr Leu Phe 
            100                 105                 110         


Phe Val Leu Gly Leu Ala Leu Met Ala Asp Gly Val Glu Ile Phe Val 
        115                 120                 125             


Val Ser Phe Ala Leu Pro Ser Ala Glu Lys Asp Met Cys Leu Ser Ser 
    130                 135                 140                 


Ser Lys Lys Gly Met Leu Gly Leu Ile Val Tyr Leu Gly Met Met Ala 
145                 150                 155                 160 


Gly Ala Phe Ile Leu Gly Gly Leu Ala Asp Lys Leu Gly Arg Lys Lys 
                165                 170                 175     


Val Leu Ser Met Ser Leu Ala Ile Asn Ala Ser Phe Ala Ser Leu Ser 
            180                 185                 190         


Ser Phe Val Gln Gly Tyr Gly Ala Phe Leu Phe Cys Arg Leu Ile Ser 
        195                 200                 205             


Gly Ile Gly Ile Gly Gly Ser Leu Pro Ile Val Phe Ala Tyr Phe Ser 
    210                 215                 220                 


Glu Phe Leu Ser Arg Glu Lys Arg Gly Glu His Leu Ser Trp Leu Gly 
225                 230                 235                 240 


Ile Phe Trp Met Thr Gly Gly Ile Tyr Ala Ser Ala Met Ala Trp Ser 
                245                 250                 255     


Ile Ile Pro His Tyr Gly Trp Gly Phe Ser Met Gly Thr Asn Tyr His 
            260                 265                 270         


Phe His Ser Trp Arg Val Phe Val Ile Val Cys Ala Leu Pro Ala Thr 
        275                 280                 285             


Val Ser Met Val Ala Leu Lys Phe Met Pro Glu Ser Pro Arg Phe Leu 
    290                 295                 300                 


Leu Glu Met Gly Lys His Asp Glu Ala Trp Met Ile Leu Lys Gln Val 
305                 310                 315                 320 


His Asp Thr Asn Met Arg Ala Lys Gly Thr Pro Glu Lys Val Phe Thr 
                325                 330                 335     


Val Ser His Ile Lys Thr Pro Lys Gln Met Asp Glu Phe Ile Glu Ile 
            340                 345                 350         


Gln Ser Ser Thr Gly Thr Trp Tyr Gln Arg Trp Leu Val Arg Phe Met 
        355                 360                 365             


Thr Ile Phe Lys Gln Val Trp Asp Asn Ala Leu Tyr Cys Val Met Gly 
    370                 375                 380                 


Pro Tyr Arg Met Asn Thr Leu Ile Leu Ala Val Val Trp Phe Thr Met 
385                 390                 395                 400 


Ala Leu Ser Tyr Tyr Gly Leu Thr Val Trp Phe Pro Asp Met Ile Arg 
                405                 410                 415     


Tyr Phe Gln Asp Glu Glu Tyr Lys Ser Lys Met Lys Val Phe Phe Gly 
            420                 425                 430         


Glu His Val His Gly Ala Thr Ile Asn Phe Thr Met Glu Asn Gln Ile 
        435                 440                 445             


His Gln His Gly Lys Leu Val Asn Asp Lys Phe Ile Lys Met Tyr Phe 
    450                 455                 460                 


Lys His Val Leu Phe Glu Asp Thr Phe Phe Asp Lys Cys Tyr Phe Glu 
465                 470                 475                 480 


Asp Val Thr Ser Thr Asp Thr Tyr Phe Lys Asn Cys Thr Ile Glu Ser 
                485                 490                 495     


Thr Thr Phe Tyr Asn Thr Asp Leu Tyr Lys His Lys Phe Ile Asn Cys 
            500                 505                 510         


Arg Phe Ile Asn Ser Thr Phe Leu Glu Gln Lys Glu Gly Cys His Met 
        515                 520                 525             


Asp Phe Glu Glu Asp Asn Asp Phe Leu Ile Tyr Leu Val Ser Phe Leu 
    530                 535                 540                 


Gly Ser Leu Ser Val Leu Pro Gly Asn Ile Ile Ser Ala Leu Leu Met 
545                 550                 555                 560 


Asp Arg Ile Gly Arg Leu Lys Met Ile Gly Gly Ser Met Leu Ile Ser 
                565                 570                 575     


Ala Val Cys Cys Phe Phe Leu Phe Phe Gly Asn Ser Glu Ser Ala Met 
            580                 585                 590         


Ile Gly Trp Gln Cys Leu Phe Cys Gly Thr Ser Ile Ala Ala Trp Asn 
        595                 600                 605             


Ala Leu Asp Val Ile Thr Val Glu Leu Tyr Pro Thr Asn Gln Arg Ala 
    610                 615                 620                 


Thr Ala Phe Gly Ile Leu Asn Gly Leu Cys Lys Phe Gly Ala Ile Leu 
625                 630                 635                 640 


Gly Asn Thr Ile Phe Ala Ser Phe Val Gly Ile Thr Lys Val Val Pro 
                645                 650                 655     


Ile Leu Leu Ala Ala Ala Ser Leu Val Gly Gly Gly Leu Ile Ala Leu 
            660                 665                 670         


Arg Leu Pro Glu Thr Arg Glu Gln Val Leu Met 
        675                 680             


<210>  30
<211>  667
<212>  PRT
<213>  Mus musculus

<400>  30

Met Glu Asp Ser Tyr Lys Asp Arg Thr Ser Leu Met Lys Gly Ala Lys 
1               5                   10                  15      


Asp Ile Ala Lys Glu Val Lys Lys Gln Thr Val Lys Lys Val Asn Gln 
            20                  25                  30          


Ala Val Asp Arg Ala Gln Asp Glu Tyr Thr Gln Arg Ser Tyr Ser Arg 
        35                  40                  45              


Phe Gln Asp Glu Glu Asp Asp Asp Asp Tyr Tyr Pro Pro Gly Glu Thr 
    50                  55                  60                  


Tyr Ser Gly Glu Val Asn Asp Asp Glu Gly Ser Ser Glu Ala Thr Glu 
65                  70                  75                  80  


Gly His Asp Glu Glu Asp Glu Ile Tyr Glu Gly Glu Tyr Gln Gly Ile 
                85                  90                  95      


Pro Ser Thr Asn Gln Gly Lys Asp Ser Ile Val Ser Val Gly Gln Pro 
            100                 105                 110         


Lys Gly Asp Glu Tyr Lys Asp Arg Arg Glu Leu Glu Ser Glu Arg Arg 
        115                 120                 125             


Ala Asp Glu Glu Glu Leu Ala Gln Gln Tyr Glu Leu Ile Ile Gln Glu 
    130                 135                 140                 


Cys Gly His Gly Arg Phe Gln Trp Ala Leu Phe Phe Val Leu Gly Met 
145                 150                 155                 160 


Ala Leu Met Ala Asp Gly Val Glu Val Phe Val Val Gly Phe Val Leu 
                165                 170                 175     


Pro Ser Ala Glu Thr Asp Leu Cys Ile Pro Asn Ser Gly Ser Gly Trp 
            180                 185                 190         


Leu Gly Ser Ile Val Tyr Leu Gly Met Met Val Gly Ala Phe Phe Trp 
        195                 200                 205             


Gly Gly Leu Ala Asp Lys Val Gly Arg Lys Gln Ser Leu Leu Ile Cys 
    210                 215                 220                 


Met Ser Val Asn Gly Phe Phe Ala Phe Leu Ser Ser Phe Val Gln Gly 
225                 230                 235                 240 


Tyr Gly Phe Phe Leu Val Cys Arg Leu Leu Ser Gly Phe Gly Ile Gly 
                245                 250                 255     


Gly Ala Ile Pro Thr Val Phe Ser Tyr Phe Ala Glu Val Leu Ala Arg 
            260                 265                 270         


Glu Lys Arg Gly Glu His Leu Ser Trp Leu Cys Met Phe Trp Met Ile 
        275                 280                 285             


Gly Gly Ile Tyr Ala Ser Ala Met Ala Trp Ala Ile Ile Pro His Tyr 
    290                 295                 300                 


Gly Trp Ser Phe Ser Met Gly Ser Ala Tyr Gln Phe His Ser Trp Arg 
305                 310                 315                 320 


Val Phe Val Ile Val Cys Ala Leu Pro Cys Val Ser Ser Val Val Ala 
                325                 330                 335     


Leu Thr Phe Met Pro Glu Ser Pro Arg Phe Leu Leu Glu Val Gly Lys 
            340                 345                 350         


His Asp Glu Ala Trp Met Ile Leu Lys Leu Ile His Asp Thr Asn Met 
        355                 360                 365             


Arg Ala Arg Gly Gln Pro Glu Lys Val Phe Thr Val Asn Lys Ile Lys 
    370                 375                 380                 


Thr Pro Lys Gln Ile Asp Glu Leu Ile Glu Ile Glu Ser Asp Thr Gly 
385                 390                 395                 400 


Thr Trp Tyr Arg Arg Cys Phe Val Arg Ile Arg Thr Glu Leu Tyr Gly 
                405                 410                 415     


Ile Trp Leu Thr Phe Met Arg Cys Phe Asn Tyr Pro Val Arg Glu Asn 
            420                 425                 430         


Thr Ile Lys Leu Thr Ile Val Trp Phe Thr Leu Ser Phe Gly Tyr Tyr 
        435                 440                 445             


Gly Leu Ser Val Trp Phe Pro Asp Val Ile Lys His Leu Gln Ser Asp 
    450                 455                 460                 


Glu Tyr Ala Leu Leu Thr Arg Asn Val Gln Lys Asp Lys Tyr Ala Asn 
465                 470                 475                 480 


Phe Ser Ile Asn Phe Thr Met Glu Asn Gln Ile His Thr Gly Met Glu 
                485                 490                 495     


Tyr Glu Asn Gly Arg Phe Leu Gly Val Lys Phe Lys Ser Val Thr Phe 
            500                 505                 510         


Lys Asp Ser Val Phe Lys Ser Cys Thr Phe Asp Asp Val Thr Ser Val 
        515                 520                 525             


Asn Thr Tyr Phe Lys Asn Cys Thr Phe Ile Asp Thr Leu Phe Asp Asn 
    530                 535                 540                 


Thr Asp Phe Glu Pro Tyr Lys Phe Ile Asp Ser Glu Phe Gln Asn Cys 
545                 550                 555                 560 


Ser Phe Leu His Asn Lys Thr Gly Cys Gln Ile Thr Phe Asp Asp Asp 
                565                 570                 575     


Tyr Ser Ala Tyr Trp Ile Tyr Phe Val Asn Phe Leu Gly Thr Leu Ala 
            580                 585                 590         


Val Leu Pro Gly Asn Ile Val Ser Ala Leu Leu Met Asp Arg Ile Gly 
        595                 600                 605             


Arg Leu Thr Met Leu Gly Gly Ser Met Val Leu Ser Gly Ile Ser Cys 
    610                 615                 620                 


Phe Phe Leu Trp Phe Gly Thr Ser Glu Ser Met Met Ile Gly Met Leu 
625                 630                 635                 640 


Cys Leu Tyr Asn Gly Leu Thr Ile Ser Ala Trp Asn Ser Leu Asp Val 
                645                 650                 655     


Val Thr Val Glu Leu Tyr Pro Thr Asp Arg Arg 
            660                 665         


<210>  31
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  31

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 3

<400>  32

Glu Ala Ala Ala Lys 
1               5   


<210>  33
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 4

<400>  33

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


