﻿               SEQUENCE LISTING

<110> UNIVERSITÉ CATHOLIQUE DE LOUVAIN
      COLLET Jean-François
      DEGHELT Michaël
      CHO Seung Hyun

<120> GENETICALLY MODIFIED BACTERIUM WITH ALTERED ENVELOP INTEGRITY AND USES THEREOF

<130> CV - 1290/PCT

<150> EP20152513.6
<151> 2020-01-17

<160> 21

<170> BiSSAP 1.3.6

<210> 1
<211> 1041
<212> DNA
<213> Escherichia coli


<220> 
<223> ompA nucleic acid sequence, 1041 bp

<400> 1
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag      60

gccgctccga aagataacac ctggtacact ggtgctaaac tgggctggtc ccagtaccat     120

gacactggtt tcatcaacaa caatggcccg acccatgaaa accaactggg cgctggtgct     180

tttggtggtt accaggttaa cccgtatgtt ggctttgaaa tgggttacga ctggttaggt     240

cgtatgccgt acaaaggcag cgttgaaaac ggtgcataca aagctcaggg cgttcaactg     300

accgctaaac tgggttaccc aatcactgac gacctggaca tctacactcg tctgggtggc     360

atggtatggc gtgcagacac taaatccaac gtttatggta aaaaccacga caccggcgtt     420

tctccggtct tcgctggcgg tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg     480

gaataccagt ggaccaacaa catcggtgac gcacacacca tcggcactcg tccggacaac     540

ggcatgctga gcctgggtgt ttcctaccgt ttcggtcagg gcgaagcagc tccagtagtt     600

gctccggctc cagctccggc accggaagta cagaccaagc acttcactct gaagtctgac     660

gttctgttca acttcaacaa agcaaccctg aaaccggaag gtcaggctgc tctggatcag     720

ctgtacagcc agctgagcaa cctggatccg aaagacggtt ccgtagttgt tctgggttac     780

accgaccgca tcggttctga cgcttacaac cagggtctgt ccgagcgccg tgctcagtct     840

gttgttgatt acctgatctc caaaggtatc ccggcagaca agatctccgc acgtggtatg     900

ggcgaatcca acccggttac tggcaacacc tgtgacaacg tgaaacagcg tgctgcactg     960

atcgactgcc tggctccgga tcgtcgcgta gagatcgaag ttaaaggtat caaagacgtt    1020

gtaactcagc cgcaggctta a                                              1041


<210> 2
<211> 346
<212> PRT
<213> Escherichia coli


<220> 
<223> OmpA preprotein amino acid sequence, 346 aa

<400> 2
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      
Thr Val Ala Gln Ala Ala Pro Lys Asp Asn Thr Trp Tyr Thr Gly Ala 
            20                  25                  30          
Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly Phe Ile Asn Asn Asn 
        35                  40                  45              
Gly Pro Thr His Glu Asn Gln Leu Gly Ala Gly Ala Phe Gly Gly Tyr 
    50                  55                  60                  
Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly 
65                  70                  75                  80  
Arg Met Pro Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln 
                85                  90                  95      
Gly Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu 
            100                 105                 110         
Asp Ile Tyr Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys 
        115                 120                 125             
Ser Asn Val Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe 
    130                 135                 140                 
Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu 
145                 150                 155                 160 
Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr 
                165                 170                 175     
Arg Pro Asp Asn Gly Met Leu Ser Leu Gly Val Ser Tyr Arg Phe Gly 
            180                 185                 190         
Gln Gly Glu Ala Ala Pro Val Val Ala Pro Ala Pro Ala Pro Ala Pro 
        195                 200                 205             
Glu Val Gln Thr Lys His Phe Thr Leu Lys Ser Asp Val Leu Phe Asn 
    210                 215                 220                 
Phe Asn Lys Ala Thr Leu Lys Pro Glu Gly Gln Ala Ala Leu Asp Gln 
225                 230                 235                 240 
Leu Tyr Ser Gln Leu Ser Asn Leu Asp Pro Lys Asp Gly Ser Val Val 
                245                 250                 255     
Val Leu Gly Tyr Thr Asp Arg Ile Gly Ser Asp Ala Tyr Asn Gln Gly 
            260                 265                 270         
Leu Ser Glu Arg Arg Ala Gln Ser Val Val Asp Tyr Leu Ile Ser Lys 
        275                 280                 285             
Gly Ile Pro Ala Asp Lys Ile Ser Ala Arg Gly Met Gly Glu Ser Asn 
    290                 295                 300                 
Pro Val Thr Gly Asn Thr Cys Asp Asn Val Lys Gln Arg Ala Ala Leu 
305                 310                 315                 320 
Ile Asp Cys Leu Ala Pro Asp Arg Arg Val Glu Ile Glu Val Lys Gly 
                325                 330                 335     
Ile Lys Asp Val Val Thr Gln Pro Gln Ala 
            340                 345     

<210> 3
<211> 325
<212> PRT
<213> Escherichia coli


<220> 
<223> OmpA mature protein amino acid sequence, 325 aa

<400> 3
Ala Pro Lys Asp Asn Thr Trp Tyr Thr Gly Ala Lys Leu Gly Trp Ser 
1               5                   10                  15      
Gln Tyr His Asp Thr Gly Phe Ile Asn Asn Asn Gly Pro Thr His Glu 
            20                  25                  30          
Asn Gln Leu Gly Ala Gly Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr 
        35                  40                  45              
Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys 
    50                  55                  60                  
Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln Leu Thr 
65                  70                  75                  80  
Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg 
                85                  90                  95      
Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val Tyr Gly 
            100                 105                 110         
Lys Asn His Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly Val Glu 
        115                 120                 125             
Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln Trp Thr 
    130                 135                 140                 
Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp Asn Gly 
145                 150                 155                 160 
Met Leu Ser Leu Gly Val Ser Tyr Arg Phe Gly Gln Gly Glu Ala Ala 
                165                 170                 175     
Pro Val Val Ala Pro Ala Pro Ala Pro Ala Pro Glu Val Gln Thr Lys 
            180                 185                 190         
His Phe Thr Leu Lys Ser Asp Val Leu Phe Asn Phe Asn Lys Ala Thr 
        195                 200                 205             
Leu Lys Pro Glu Gly Gln Ala Ala Leu Asp Gln Leu Tyr Ser Gln Leu 
    210                 215                 220                 
Ser Asn Leu Asp Pro Lys Asp Gly Ser Val Val Val Leu Gly Tyr Thr 
225                 230                 235                 240 
Asp Arg Ile Gly Ser Asp Ala Tyr Asn Gln Gly Leu Ser Glu Arg Arg 
                245                 250                 255     
Ala Gln Ser Val Val Asp Tyr Leu Ile Ser Lys Gly Ile Pro Ala Asp 
            260                 265                 270         
Lys Ile Ser Ala Arg Gly Met Gly Glu Ser Asn Pro Val Thr Gly Asn 
        275                 280                 285             
Thr Cys Asp Asn Val Lys Gln Arg Ala Ala Leu Ile Asp Cys Leu Ala 
    290                 295                 300                 
Pro Asp Arg Arg Val Glu Ile Glu Val Lys Gly Ile Lys Asp Val Val 
305                 310                 315                 320 
Thr Gln Pro Gln Ala 
                325 

<210> 4
<211> 237
<212> DNA
<213> Escherichia coli


<220> 
<223> lpp nucleic acid sequence, 237 bp

<400> 4
atgaaagcta ctaaactggt actgggcgcg gtaatcctgg gttctactct gctggcaggt     60

tgctccagca acgctaaaat cgatcagctg tcttctgacg ttcagactct gaacgctaaa    120

gttgaccagc tgagcaacga cgtgaacgca atgcgttccg acgttcaggc tgctaaagat    180

gacgcagctc gtgctaacca gcgtctggac aacatggcta ctaaataccg caagtaa       237


<210> 5
<211> 78
<212> PRT
<213> Escherichia coli


<220> 
<223> Lpp preprotein amino acid sequence, 78 aa

<400> 5
Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      
Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Leu Ser Ser 
            20                  25                  30          
Asp Val Gln Thr Leu Asn Ala Lys Val Asp Gln Leu Ser Asn Asp Val 
        35                  40                  45              
Asn Ala Met Arg Ser Asp Val Gln Ala Ala Lys Asp Asp Ala Ala Arg 
    50                  55                  60                  
Ala Asn Gln Arg Leu Asp Asn Met Ala Thr Lys Tyr Arg Lys 
65                  70                  75              

<210> 6
<211> 58
<212> PRT
<213> Escherichia coli


<220> 
<223> Lpp mature protein amino acid sequence, 58 aa

<400> 6
Cys Ser Ser Asn Ala Lys Ile Asp Gln Leu Ser Ser Asp Val Gln Thr 
1               5                   10                  15      
Leu Asn Ala Lys Val Asp Gln Leu Ser Asn Asp Val Asn Ala Met Arg 
            20                  25                  30          
Ser Asp Val Gln Ala Ala Lys Asp Asp Ala Ala Arg Ala Asn Gln Arg 
        35                  40                  45              
Leu Asp Asn Met Ala Thr Lys Tyr Arg Lys 
    50                  55              

<210> 7
<211> 522
<212> DNA
<213> Escherichia coli


<220> 
<223> pal nucleic acid sequence, 522 bp

<400> 7
atgcaactga acaaagtgct gaaagggctg atgattgctc tgcctgttat ggcaattgcg      60

gcatgttctt ccaacaagaa cgccagcaat gacggcagcg aaggcatgct gggtgccggc     120

actggtatgg atgcgaacgg cggcaacggc aacatgtctt ccgaagagca ggctcgtctg     180

caaatgcaac agctgcagca gaacaacatc gtttacttcg atctggacaa gtacgatatc     240

cgttctgact tcgctcaaat gctggatgca catgcaaact tcctgcgtag caacccgtct     300

tacaaagtca ccgtagaagg tcacgcggac gaacgtggta ctccggaata caacatctcc     360

ctgggtgaac gtcgtgcgaa cgccgttaag atgtacctgc agggtaaagg cgtttctgca     420

gaccagatct ccatcgtttc ttacggtaaa gaaaaacctg cagtactggg tcatgacgaa     480

gcggcatact ccaaaaaccg tcgtgcggta ctggtttact aa                        522


<210> 8
<211> 173
<212> PRT
<213> Escherichia coli


<220> 
<223> Pal preprotein amino acid sequence, 173 aa

<400> 8
Met Gln Leu Asn Lys Val Leu Lys Gly Leu Met Ile Ala Leu Pro Val 
1               5                   10                  15      
Met Ala Ile Ala Ala Cys Ser Ser Asn Lys Asn Ala Ser Asn Asp Gly 
            20                  25                  30          
Ser Glu Gly Met Leu Gly Ala Gly Thr Gly Met Asp Ala Asn Gly Gly 
        35                  40                  45              
Asn Gly Asn Met Ser Ser Glu Glu Gln Ala Arg Leu Gln Met Gln Gln 
    50                  55                  60                  
Leu Gln Gln Asn Asn Ile Val Tyr Phe Asp Leu Asp Lys Tyr Asp Ile 
65                  70                  75                  80  
Arg Ser Asp Phe Ala Gln Met Leu Asp Ala His Ala Asn Phe Leu Arg 
                85                  90                  95      
Ser Asn Pro Ser Tyr Lys Val Thr Val Glu Gly His Ala Asp Glu Arg 
            100                 105                 110         
Gly Thr Pro Glu Tyr Asn Ile Ser Leu Gly Glu Arg Arg Ala Asn Ala 
        115                 120                 125             
Val Lys Met Tyr Leu Gln Gly Lys Gly Val Ser Ala Asp Gln Ile Ser 
    130                 135                 140                 
Ile Val Ser Tyr Gly Lys Glu Lys Pro Ala Val Leu Gly His Asp Glu 
145                 150                 155                 160 
Ala Ala Tyr Ser Lys Asn Arg Arg Ala Val Leu Val Tyr 
                165                 170             

<210> 9
<211> 152
<212> PRT
<213> Escherichia coli


<220> 
<223> Pal mature protein amino acid sequence, 152 aa

<400> 9
Cys Ser Ser Asn Lys Asn Ala Ser Asn Asp Gly Ser Glu Gly Met Leu 
1               5                   10                  15      
Gly Ala Gly Thr Gly Met Asp Ala Asn Gly Gly Asn Gly Asn Met Ser 
            20                  25                  30          
Ser Glu Glu Gln Ala Arg Leu Gln Met Gln Gln Leu Gln Gln Asn Asn 
        35                  40                  45              
Ile Val Tyr Phe Asp Leu Asp Lys Tyr Asp Ile Arg Ser Asp Phe Ala 
    50                  55                  60                  
Gln Met Leu Asp Ala His Ala Asn Phe Leu Arg Ser Asn Pro Ser Tyr 
65                  70                  75                  80  
Lys Val Thr Val Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr 
                85                  90                  95      
Asn Ile Ser Leu Gly Glu Arg Arg Ala Asn Ala Val Lys Met Tyr Leu 
            100                 105                 110         
Gln Gly Lys Gly Val Ser Ala Asp Gln Ile Ser Ile Val Ser Tyr Gly 
        115                 120                 125             
Lys Glu Lys Pro Ala Val Leu Gly His Asp Glu Ala Ala Tyr Ser Lys 
    130                 135                 140                 
Asn Arg Arg Ala Val Leu Val Tyr 
145                 150         

<210> 10
<211> 921
<212> DNA
<213> Escherichia coli


<220> 
<223> ybiS nucleic acid sequence, 921 bp

<400> 10
atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg cttttgcagt      60

accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt tggtcagaat     120

caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc cgccgagtac     180

cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt cctgccgaaa     240

ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt tcatgaaggc     300

atcgtcatta acagtgctga gatgcgtctt tattactatc cgaaagggac caacaccgtt     360

atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa ctggaccacc     420

aaagttgagc gtaaaaaagc aggcccgacc tggacgccga ccgccaaaat gcacgcagag     480

taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga taacccgatg     540

gggctgtatg cactctatat cggtcgcctg tatgctatcc atggcaccaa cgccaacttc     600

ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga catcaaattc     660

ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc ggtaaaagcg     720

accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc taccaccgaa     780

gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt gcagacagtg     840

accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa ccgctccggg     900

atgccggttc gtctgaatta a                                               921


<210> 11
<211> 306
<212> PRT
<213> Escherichia coli


<220> 
<223> YbiS protein amino acid sequence, 306 aa

<400> 11
Met Asn Met Lys Leu Lys Thr Leu Phe Ala Ala Ala Phe Ala Val Val 
1               5                   10                  15      
Gly Phe Cys Ser Thr Ala Ser Ala Val Thr Tyr Pro Leu Pro Thr Asp 
            20                  25                  30          
Gly Ser Arg Leu Val Gly Gln Asn Gln Val Ile Thr Ile Pro Glu Gly 
        35                  40                  45              
Asn Thr Gln Pro Leu Glu Tyr Phe Ala Ala Glu Tyr Gln Met Gly Leu 
    50                  55                  60                  
Ser Asn Met Met Glu Ala Asn Pro Gly Val Asp Thr Phe Leu Pro Lys 
65                  70                  75                  80  
Gly Gly Thr Val Leu Asn Ile Pro Gln Gln Leu Ile Leu Pro Asp Thr 
                85                  90                  95      
Val His Glu Gly Ile Val Ile Asn Ser Ala Glu Met Arg Leu Tyr Tyr 
            100                 105                 110         
Tyr Pro Lys Gly Thr Asn Thr Val Ile Val Leu Pro Ile Gly Ile Gly 
        115                 120                 125             
Gln Leu Gly Lys Asp Thr Pro Ile Asn Trp Thr Thr Lys Val Glu Arg 
    130                 135                 140                 
Lys Lys Ala Gly Pro Thr Trp Thr Pro Thr Ala Lys Met His Ala Glu 
145                 150                 155                 160 
Tyr Arg Ala Ala Gly Glu Pro Leu Pro Ala Val Val Pro Ala Gly Pro 
                165                 170                 175     
Asp Asn Pro Met Gly Leu Tyr Ala Leu Tyr Ile Gly Arg Leu Tyr Ala 
            180                 185                 190         
Ile His Gly Thr Asn Ala Asn Phe Gly Ile Gly Leu Arg Val Ser His 
        195                 200                 205             
Gly Cys Val Arg Leu Arg Asn Glu Asp Ile Lys Phe Leu Phe Glu Lys 
    210                 215                 220                 
Val Pro Val Gly Thr Arg Val Gln Phe Ile Asp Glu Pro Val Lys Ala 
225                 230                 235                 240 
Thr Thr Glu Pro Asp Gly Ser Arg Tyr Ile Glu Val His Asn Pro Leu 
                245                 250                 255     
Ser Thr Thr Glu Ala Gln Phe Glu Gly Gln Glu Ile Val Pro Ile Thr 
            260                 265                 270         
Leu Thr Lys Ser Val Gln Thr Val Thr Gly Gln Pro Asp Val Asp Gln 
        275                 280                 285             
Val Val Leu Asp Glu Ala Ile Lys Asn Arg Ser Gly Met Pro Val Arg 
    290                 295                 300                 
Leu Asn 
305     

<210> 12
<211> 963
<212> DNA
<213> Escherichia coli


<220> 
<223> ycfS nucleic acid sequence, 963 bp

<400> 12
gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg      60

gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg     120

gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc     180

aaaaaataca acgtcggctt tctcgctctg ttacaggcta accccggcgt tgatccttac     240

gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agatgcgccg     300

cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa     360

aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg     420

acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac     480

atccgcgccc gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggactg     540

gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt     600

catggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg     660

cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc     720

atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat     780

cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc     840

gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg     900

gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg     960

taa                                                                   963


<210> 13
<211> 320
<212> PRT
<213> Escherichia coli


<220> 
<223> YcfS protein amino acid sequence, 320 aa

<400> 13
Val Met Ile Lys Thr Arg Phe Ser Arg Trp Leu Thr Phe Phe Thr Phe 
1               5                   10                  15      
Ala Ala Ala Val Ala Leu Ala Leu Pro Ala Lys Ala Asn Thr Trp Pro 
            20                  25                  30          
Leu Pro Pro Ala Gly Ser Arg Leu Val Gly Glu Asn Lys Phe His Val 
        35                  40                  45              
Val Glu Asn Asp Gly Gly Ser Leu Glu Ala Ile Ala Lys Lys Tyr Asn 
    50                  55                  60                  
Val Gly Phe Leu Ala Leu Leu Gln Ala Asn Pro Gly Val Asp Pro Tyr 
65                  70                  75                  80  
Val Pro Arg Ala Gly Ser Val Leu Thr Ile Pro Leu Gln Thr Leu Leu 
                85                  90                  95      
Pro Asp Ala Pro Arg Glu Gly Ile Val Ile Asn Ile Ala Glu Leu Arg 
            100                 105                 110         
Leu Tyr Tyr Tyr Pro Pro Gly Lys Asn Ser Val Thr Val Tyr Pro Ile 
        115                 120                 125             
Gly Ile Gly Gln Leu Gly Gly Asp Thr Leu Thr Pro Thr Met Val Thr 
    130                 135                 140                 
Thr Val Ser Asp Lys Arg Ala Asn Pro Thr Trp Thr Pro Thr Ala Asn 
145                 150                 155                 160 
Ile Arg Ala Arg Tyr Lys Ala Gln Gly Ile Glu Leu Pro Ala Val Val 
                165                 170                 175     
Pro Ala Gly Leu Asp Asn Pro Met Gly His His Ala Ile Arg Leu Ala 
            180                 185                 190         
Ala Tyr Gly Gly Val Tyr Leu Leu His Gly Thr Asn Ala Asp Phe Gly 
        195                 200                 205             
Ile Gly Met Arg Val Ser Ser Gly Cys Ile Arg Leu Arg Asp Asp Asp 
    210                 215                 220                 
Ile Lys Thr Leu Phe Ser Gln Val Thr Pro Gly Thr Lys Val Asn Ile 
225                 230                 235                 240 
Ile Asn Thr Pro Ile Lys Val Ser Ala Glu Pro Asn Gly Ala Arg Leu 
                245                 250                 255     
Val Glu Val His Gln Pro Leu Ser Glu Lys Ile Asp Asp Asp Pro Gln 
            260                 265                 270         
Leu Leu Pro Ile Thr Leu Asn Ser Ala Met Gln Ser Phe Lys Asp Ala 
        275                 280                 285             
Ala Gln Thr Asp Ala Glu Val Met Gln His Val Met Asp Val Arg Ser 
    290                 295                 300                 
Gly Met Pro Val Asp Val Arg Arg His Gln Val Ser Pro Gln Thr Leu 
305                 310                 315                 320 



<210> 14
<211> 933
<212> DNA
<213> Escherichia coli


<220> 
<223> erfK nucleic acid sequence, 933 bp

<400> 14
atgcgtcgtg taaatattct ttgctcattt gctctgcttt ttgccagcca tactagcctg      60

gcggtaactt atccattacc tccagagggt agccgtttag tggggcagtc gtttactgta     120

actgttcctg atcacaatac ccagccgctg gagacttttg ccgcacaata cgggcaaggg     180

ttaagtaaca tgctggaagc gaacccgggc gctgatgttt ttttgccgaa gtctggctcg     240

caactcacca ttccgcagca actgattttg cccgacactg ttcgtaaagg gattgttgtt     300

aacgtcgctg agatgcgtct ttattactac ccaccagaca gtaatactgt ggaagtcttt     360

cctattggta tcggccaggc tgggcgagaa accccgcgta actgggtgac taccgttgaa     420

cgtaaacaag aagctccaac ctggacgcca acgccgaaca ctcggcgcga atatgcgaaa     480

cgaggggaga gtttgcccgc atttgttcct gcgggccccg ataatcccat ggggctgtac     540

gcgatttata ttggcaggtt gtatgccatc catggtacca atgccaattt tggtattggg     600

ctccgggtaa gtcagggctg tattcgtctg cgcaatgacg atatcaaata tctgtttgat     660

aatgttcctg ttgggacgcg tgtgcaaatt atcgaccagc cagtaaaata caccactgaa     720

ccagatggct cgaactggct ggaagttcat gagccattgt cgcgcaatcg tgcagaatat     780

gagtctgacc gaaaagtgcc attgccggta accccatctt tgcgggcgtt tatcaacggg     840

caagaagttg atgtaaatcg cgcaaatgct gcgttgcaac gtcgatcggg aatgcctgtg     900

caaattagtt ctggttcaag acagatgttt taa                                  933


<210> 15
<211> 310
<212> PRT
<213> Escherichia coli


<220> 
<223> ErfK protein amino acid sequence, 310 aa

<400> 15
Met Arg Arg Val Asn Ile Leu Cys Ser Phe Ala Leu Leu Phe Ala Ser 
1               5                   10                  15      
His Thr Ser Leu Ala Val Thr Tyr Pro Leu Pro Pro Glu Gly Ser Arg 
            20                  25                  30          
Leu Val Gly Gln Ser Phe Thr Val Thr Val Pro Asp His Asn Thr Gln 
        35                  40                  45              
Pro Leu Glu Thr Phe Ala Ala Gln Tyr Gly Gln Gly Leu Ser Asn Met 
    50                  55                  60                  
Leu Glu Ala Asn Pro Gly Ala Asp Val Phe Leu Pro Lys Ser Gly Ser 
65                  70                  75                  80  
Gln Leu Thr Ile Pro Gln Gln Leu Ile Leu Pro Asp Thr Val Arg Lys 
                85                  90                  95      
Gly Ile Val Val Asn Val Ala Glu Met Arg Leu Tyr Tyr Tyr Pro Pro 
            100                 105                 110         
Asp Ser Asn Thr Val Glu Val Phe Pro Ile Gly Ile Gly Gln Ala Gly 
        115                 120                 125             
Arg Glu Thr Pro Arg Asn Trp Val Thr Thr Val Glu Arg Lys Gln Glu 
    130                 135                 140                 
Ala Pro Thr Trp Thr Pro Thr Pro Asn Thr Arg Arg Glu Tyr Ala Lys 
145                 150                 155                 160 
Arg Gly Glu Ser Leu Pro Ala Phe Val Pro Ala Gly Pro Asp Asn Pro 
                165                 170                 175     
Met Gly Leu Tyr Ala Ile Tyr Ile Gly Arg Leu Tyr Ala Ile His Gly 
            180                 185                 190         
Thr Asn Ala Asn Phe Gly Ile Gly Leu Arg Val Ser Gln Gly Cys Ile 
        195                 200                 205             
Arg Leu Arg Asn Asp Asp Ile Lys Tyr Leu Phe Asp Asn Val Pro Val 
    210                 215                 220                 
Gly Thr Arg Val Gln Ile Ile Asp Gln Pro Val Lys Tyr Thr Thr Glu 
225                 230                 235                 240 
Pro Asp Gly Ser Asn Trp Leu Glu Val His Glu Pro Leu Ser Arg Asn 
                245                 250                 255     
Arg Ala Glu Tyr Glu Ser Asp Arg Lys Val Pro Leu Pro Val Thr Pro 
            260                 265                 270         
Ser Leu Arg Ala Phe Ile Asn Gly Gln Glu Val Asp Val Asn Arg Ala 
        275                 280                 285             
Asn Ala Ala Leu Gln Arg Arg Ser Gly Met Pro Val Gln Ile Ser Ser 
    290                 295                 300                 
Gly Ser Arg Gln Met Phe 
305                 310 

<210> 16
<211> 483
<212> DNA
<213> Escherichia coli


<220> 
<223> yfiB nucleic acid, 483 bp

<400> 16
atgataaagc acctggtagc acccctggtt ttcacctcac taatactgac tggctgccag      60

tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta tggatttact     120

gaatccgccg gcgactggtc gctgggctta tcagatgcca ttctgttcgc aaaaaatgac     180

tacaaattgc tcccggaaag ccagcaacag atccaaacca tggcagctaa attggcctcg     240

acagggctaa cacatgcccg tatggatgga cacaccgata actatggtga agacagttac     300

aacgaaggct tatcattgaa acgggcgaat gtcgtggccg atgcatgggc tatgggtgga     360

caaattccac gcagcaatct caccacacag ggtttaggaa aaaaatatcc catagccagt     420

aacaagaccg cccagggccg cgccgagaac cgccgcgtcg cagtggtgat tactacccct     480

taa                                                                   483


<210> 17
<211> 160
<212> PRT
<213> Escherichia coli


<220> 
<223> YfiB protein amino acid sequence, 160 aa

<400> 17
Met Ile Lys His Leu Val Ala Pro Leu Val Phe Thr Ser Leu Ile Leu 
1               5                   10                  15      
Thr Gly Cys Gln Ser Pro Gln Gly Lys Phe Thr Pro Glu Gln Val Ala 
            20                  25                  30          
Ala Met Gln Ser Tyr Gly Phe Thr Glu Ser Ala Gly Asp Trp Ser Leu 
        35                  40                  45              
Gly Leu Ser Asp Ala Ile Leu Phe Ala Lys Asn Asp Tyr Lys Leu Leu 
    50                  55                  60                  
Pro Glu Ser Gln Gln Gln Ile Gln Thr Met Ala Ala Lys Leu Ala Ser 
65                  70                  75                  80  
Thr Gly Leu Thr His Ala Arg Met Asp Gly His Thr Asp Asn Tyr Gly 
                85                  90                  95      
Glu Asp Ser Tyr Asn Glu Gly Leu Ser Leu Lys Arg Ala Asn Val Val 
            100                 105                 110         
Ala Asp Ala Trp Ala Met Gly Gly Gln Ile Pro Arg Ser Asn Leu Thr 
        115                 120                 125             
Thr Gln Gly Leu Gly Lys Lys Tyr Pro Ile Ala Ser Asn Lys Thr Ala 
    130                 135                 140                 
Gln Gly Arg Ala Glu Asn Arg Arg Val Ala Val Val Ile Thr Thr Pro 
145                 150                 155                 160 



<210> 18
<211> 660
<212> DNA
<213> Escherichia coli


<220> 
<223> yiaD nucleic acid, 660 bp

<400> 18
atgaagaaac gtgtttatct tattgccgcc gtagtgagtg gcgctctggc ggtatctggc      60

tgcacaacta acccttacac cggcgaacgc gaagcaggta aatctgctat cggcgcaggt     120

ctgggctctc tcgtgggcgc gggtattggt gcgctctctt cttcgaagaa agatcgcggt     180

aaaggcgcgc tgattggcgc agcagcaggc gcagctctgg gcggcggcgt tggttattac     240

atggatgtgc aggaagcgaa gctgcgcgac aaaatgcgcg gcactggtgt tagcgtaacc     300

cgcagcgggg ataacattat cctcaatatg ccgaacaatg tgaccttcga cagcagcagc     360

gcgaccctga aaccggcggg cgctaacacc ctgaccggcg tggcaatggt actgaaagag     420

tatccgaaaa cggcggttaa cgtgattggt tataccgaca gcacgggtgg tcacgacctg     480

aacatgcgtc tctcccagca acgtgcggat tccgttgcca gcgcgttgat cacccagggc     540

gtggacgcca gccgcatccg tactcagggc cttggcccgg ctaacccaat cgccagcaac     600

agcaccgcag aaggtaaggc gcaaaaccgc cgtgtagaaa ttaccttaag cccgctgtaa     660


<210> 19
<211> 219
<212> PRT
<213> Escherichia coli


<220> 
<223> YiaD protein amino acid sequence, 219 aa

<400> 19
Met Lys Lys Arg Val Tyr Leu Ile Ala Ala Val Val Ser Gly Ala Leu 
1               5                   10                  15      
Ala Val Ser Gly Cys Thr Thr Asn Pro Tyr Thr Gly Glu Arg Glu Ala 
            20                  25                  30          
Gly Lys Ser Ala Ile Gly Ala Gly Leu Gly Ser Leu Val Gly Ala Gly 
        35                  40                  45              
Ile Gly Ala Leu Ser Ser Ser Lys Lys Asp Arg Gly Lys Gly Ala Leu 
    50                  55                  60                  
Ile Gly Ala Ala Ala Gly Ala Ala Leu Gly Gly Gly Val Gly Tyr Tyr 
65                  70                  75                  80  
Met Asp Val Gln Glu Ala Lys Leu Arg Asp Lys Met Arg Gly Thr Gly 
                85                  90                  95      
Val Ser Val Thr Arg Ser Gly Asp Asn Ile Ile Leu Asn Met Pro Asn 
            100                 105                 110         
Asn Val Thr Phe Asp Ser Ser Ser Ala Thr Leu Lys Pro Ala Gly Ala 
        115                 120                 125             
Asn Thr Leu Thr Gly Val Ala Met Val Leu Lys Glu Tyr Pro Lys Thr 
    130                 135                 140                 
Ala Val Asn Val Ile Gly Tyr Thr Asp Ser Thr Gly Gly His Asp Leu 
145                 150                 155                 160 
Asn Met Arg Leu Ser Gln Gln Arg Ala Asp Ser Val Ala Ser Ala Leu 
                165                 170                 175     
Ile Thr Gln Gly Val Asp Ala Ser Arg Ile Arg Thr Gln Gly Leu Gly 
            180                 185                 190         
Pro Ala Asn Pro Ile Ala Ser Asn Ser Thr Ala Glu Gly Lys Ala Gln 
        195                 200                 205             
Asn Arg Arg Val Glu Ile Thr Leu Ser Pro Leu 
    210                 215                 

<210> 20
<211> 258
<212> DNA
<213> Escherichia coli


<220> 
<223> yqhH nucleic acid, 258 bp

<400> 20
atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc     60

gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc    120

gcccggcttg agcaggatat gaaagcacta cgcccacaaa tctatgctgc caaatccgaa    180

gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct gcgctgcttg    240

cgtatgtacg cagaatga                                                  258


<210> 21
<211> 85
<212> PRT
<213> Escherichia coli


<220> 
<223> YqhH protein amino acid sequence, 85 aa

<400> 21
Met Lys Thr Ile Phe Thr Val Gly Ala Val Val Leu Ala Thr Cys Leu 
1               5                   10                  15      
Leu Ser Gly Cys Val Asn Glu Gln Lys Val Asn Gln Leu Ala Ser Asn 
            20                  25                  30          
Val Gln Thr Leu Asn Ala Lys Ile Ala Arg Leu Glu Gln Asp Met Lys 
        35                  40                  45              
Ala Leu Arg Pro Gln Ile Tyr Ala Ala Lys Ser Glu Ala Asn Arg Ala 
    50                  55                  60                  
Asn Thr Arg Leu Asp Ala Gln Asp Tyr Phe Asp Cys Leu Arg Cys Leu 
65                  70                  75                  80  
Arg Met Tyr Ala Glu 
                85  

