                         SEQUENCE LISTING

<110>  THE INSTITUTE OF CANCER RESEARCH
 
<120>  MONITORING TUMOUR EVOLUTION

<130>  007844293

<140>  PCT/EP2021/050851
<141>  2021-01-15

<150>  GB 2000747.2
<151>  2020-01-17

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRX2 Methylation Clock

<400>  1
gcacatcctg ccaggactgg agctgcgggg acctttcagt cttccacgga tgctcctctg       60

cacgtgtttt ccaggaaaac atttaggcct cggaagcgtc tttgagagcc tagaaatgcc      120

ggttgagcgt gaaatccagt ccaacctact cacagagtac tagacctaga aacgacgttt      180

gaaagagatg tgggttccat                                                  200


<210>  2
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454 Methylation Clock

<400>  2
accaggtctc tgccttgcac taaggcgcag gaagatgcag atcagcgaaa agctgaagat       60

ggggcaaggg aggttagtcc aggggagcga cgcccgatcg ctgacgagag aggatcggga      120

gggtccggaa gacggagtgg gaggcgacag ggcgcgttcc tgactgggac cgcgccgaaa      180

aggcctggaa ggcaacagaa                                                  200


<210>  3
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454-short Methylation Clock

<400>  3
gggaggttag tccaggggag cgacgcccga tcgctgacga gagaggatcg ggagggtccg       60

gaagacggag tgggaggcga cagggcgcgt tcctgactgg gaccgcgccg aaaaggcctg      120

gaaggcaaca gaa                                                         133


<210>  4
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NETO1 Methylation Clock

<400>  4
gtctttgcag gagtaatttg gtggggggac tggacatcag gcccagtgct caaccgcgaa       60

acttttacgg ccgcctttcc ctggggcgcg cccctgccgt gtctcccaag ctgagcgcgg      120

agtccgcggc gaaacctgcg ccccagggag ggagggagag                            160


<210>  5
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NETO1-short Methylation Clock

<400>  5
ggtgggggga ctggacatca ggcccagtgc tcaaccgcga aacttttacg gccgcctttc       60

cctggggcgc gcccctgccg tgtctcccaa gctgagcgcg gagtccgcgg cgaaacctgc      120

gccccaggga gggagggaga g                                                141


<210>  6
<211>  250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHAT Methylation Clock

<400>  6
gccctgagag cctcaagcca gcgttcgcgc aacactccat gtcccgaaga ccgtagtctg       60

atgatcgagg cagagtcttc ccatcatagc gtgaagaggc ggctggtcag ggtcgcctgt      120

gtgttagctg cgcctgtgag ggtctgctct gctgttgtgc gcatgtctgt cctcgatctg      180

cgagtcgggc tgtgtgtgcc tgggcggagt ctgcctttgt cggtctgtct gtgctatttc      240

tgtgtggggg                                                             250


<210>  7
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NEUROG2 Methylation Clock

<400>  7
gaaccagaca gctgtggcct gcctggtaga aacgaccctg cgataacgca gttgctagta       60

cagctctcgg cggaggagcc agggccgggt ctccaggccg ggagggggcg gccgcggtgc      120

gcccgggcag cgctccctgc gcaagtagtt cttctatgtc tttacacg                   168


<210>  8
<211>  259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCTEX1D1 Methylation Clock

<400>  8
ggcagctcag agggccactc agcggccaag tccggccggg gagggccgag ggctctgttt       60

gaggtcaccc tggagaccgg cctcagagtc cagggagagt gcgcgggcgg ccgccggctg      120

aatgaagcct gggacgcggg agccgcgccg cgcgcagtgt ctgcagtgcc ggaggtctgg      180

gaggctccgg gcgaagcctc cctgctgcag gtagggtcgc ctctctctgc agcgcgtctg      240

gaccccaaag gaaggatag                                                   259


<210>  9
<211>  254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SOX21 Methylation Clock

<400>  9
ggaggagaga ttaataaagt ttggacggct ccctcggcct ccgctgggcc gcgggcgcaa       60

gaaagacgcg gaccctcgag gcggacgagt tgcgggaact tcgcttgcct ggaggacacg      120

gcggcccagg cggccgggag cgccggcgcc gagccgcgct ctgattggct ggcgcgaccg      180

tccctatctg ctccggggag agtggaatct tctctccatc ttctgcgccc cgagcctggg      240

gtgagaggga gatc                                                        254


<210>  10
<211>  272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFAP2B Methylation Clock

<400>  10
gacaccagtt gggagactgg gtaataacac acgctccggg cacagggacc gcgggccaac       60

gaaccgcgcg tgcgccgcgc cagcctgcgt cgagccgtcg cacacggctc cgggagcccg      120

cgtctaggca cgctctccag gttgccaagc agggtgtcaa caagtgcgca cgcgcggacg      180

cccacgcagg cgcacgcgcc gtggcgcccc cgggcgtaac cccagctgct gtgacgggcc      240

agggctcgca gctcgcctgc tagagtttgt gg                                    272


<210>  11
<211>  272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLRA3 Methylation Clock

<400>  11
ggaggaagaa gagcagcaac agccggaaag aggacgcctc tccacctgct ccaagcgccg       60

cccgccggaa tccagctctc caccttgggt taaaacccga gacccggagc gcggcaaagg      120

gaagcggggg tggggccgga caacccggtc ctgtcgacca atcacgaccg cacagcggga      180

cggagtgaca ggccacttac gacggtaggc ggggcttagc gcttcaccct ttaacaaaac      240

ccaaacaaac caaacggcga ctggagcgag gt                                    272


<210>  12
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FBXO39 Methylation Clock

<400>  12
gtcttccctg tgcgcagaag caagggcaga gccccaaacc tgcagttgcc gtcttccctg       60

tgcgcagaag caagggcggg ggcgtgccat cctcacccgg agcgccggcc gagcctcccg      120

cctcccaacg cctggccagg gccccgtctc gccctctgca catgggatcc actgaccagg      180

caactgcagg tttggggctc tg                                               202


<210>  13
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HTR7 Methylation Clock

<400>  13
tctagcttga tcctcccagg aaaggcgagc gcgcggggct gagctgccag ccggtcccca       60

gccggagcct gggacggggc ggtccggccc ttacctgtca atgctgatca cgcacagggt      120

catgatcgag gccgtgcagc acatgacgtc catggcgatg aagacattac agaaaaagtg      180

tcc                                                                    183


<210>  14
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SLCA7 Methylation Clock

<400>  14
tgcaagaggc tacaaagtct gggcgcagga aatgggtaga gggggccgag gaaaggccgc       60

agggggccgg gagagcatcg ggctgctgcg gagaggaact tgcctgcttg g               111


<210>  15
<211>  194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPR78 Methylation Clock

<400>  15
ggcccagaga cagattcacc aggaggacgc ctgaggctcg agtgcggagc tcagcgctgt       60

aggcgcaaca aagcagcacc agtgcgttgg atagcagcgc cacggccagt accatcacca      120

ggagacccgc cagcagcgcc tcgccggggc ccatggcgct agcggctcgc caggcaccct      180

ggggttctca tggc                                                        194


<210>  16
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRX2_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  16
gtatattttg ttaggattgg agttgcgggg attttttagt tttttacgga tgtttttttg       60

tacgtgtttt ttaggaaaat atttaggttt cggaagcgtt tttgagagtt tagaaatgtc      120

ggttgagcgt gaaatttagt ttaatttatt tatagagtat tagatttaga aacgacgttt      180

gaaagagatg tgggttttat                                                  200


<210>  17
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  17
attaggtttt tgttttgtat taaggcgtag gaagatgtag attagcgaaa agttgaagat       60

ggggtaaggg aggttagttt aggggagcga cgttcgatcg ttgacgagag aggatcggga      120

gggttcggaa gacggagtgg gaggcgatag ggcgcgtttt tgattgggat cgcgtcgaaa      180

aggtttggaa ggtaatagaa                                                  200


<210>  18
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454_BC -short Methylation Clock (non- CpG cytosines 
       deaminated)

<400>  18
gggaggttag tttaggggag cgacgttcga tcgttgacga gagaggatcg ggagggttcg       60

gaagacggag tgggaggcga tagggcgcgt ttttgattgg gatcgcgtcg aaaaggtttg      120

gaaggtaata gaa                                                         133


<210>  19
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NETO1_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  19
gtttttgtag gagtaatttg gtggggggat tggatattag gtttagtgtt taatcgcgaa       60

atttttacgg tcgttttttt ttggggcgcg tttttgtcgt gttttttaag ttgagcgcgg      120

agttcgcggc gaaatttgcg ttttagggag ggagggagag                            160


<210>  20
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NETO1_BC-short Methylation Clock (non- CpG cytosines deaminated)

<400>  20
ggtgggggga ttggatatta ggtttagtgt ttaatcgcga aatttttacg gtcgtttttt       60

tttggggcgc gtttttgtcg tgttttttaa gttgagcgcg gagttcgcgg cgaaatttgc      120

gttttaggga gggagggaga g                                                141


<210>  21
<211>  243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHAT_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  21
gttttgagag ttttaagtta gcgttcgcgt aatattttat gtttcgaaga tcgtagtttg       60

atgatcgagg tagagttttt ttattatagc gtgaagaggc ggttggttag ggtcgtttgt      120

gtgttagttg cgtttgtgag ggtttgtttt gttgttgtgc gtatgtttgt tttcgatttg      180

cgagtcgggt tgtgtgtgtt tgggcggagt ttgtttttgt cggtttgttt gtgttatttt      240

tgt                                                                    243


<210>  22
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NEUROG2_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  22
gaattagata gttgtggttt gtttggtaga aacgattttg cgataacgta gttgttagta       60

tagttttcgg cggaggagtt agggtcgggt ttttaggtcg ggagggggcg gtcgcggtgc      120

gttcgggtag cgttttttgc gtaagtagtt tttttatgtt tttatacg                   168


<210>  23
<211>  259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCTEX1D1_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  23
ggtagtttag agggttattt agcggttaag ttcggtcggg gagggtcgag ggttttgttt       60

gaggttattt tggagatcgg ttttagagtt tagggagagt gcgcgggcgg tcgtcggttg      120

aatgaagttt gggacgcggg agtcgcgtcg cgcgtagtgt ttgtagtgtc ggaggtttgg      180

gaggtttcgg gcgaagtttt tttgttgtag gtagggtcgt ttttttttgt agcgcgtttg      240

gattttaaag gaaggatag                                                   259


<210>  24
<211>  254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SOX21_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  24
ggaggagaga ttaataaagt ttggacggtt ttttcggttt tcgttgggtc gcgggcgtaa       60

gaaagacgcg gattttcgag gcggacgagt tgcgggaatt tcgtttgttt ggaggatacg      120

gcggtttagg cggtcgggag cgtcggcgtc gagtcgcgtt ttgattggtt ggcgcgatcg      180

tttttatttg tttcggggag agtggaattt tttttttatt ttttgcgttt cgagtttggg      240

gtgagaggga gatt                                                        254


<210>  25
<211>  272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFAP2B_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  25
gatattagtt gggagattgg gtaataatat acgtttcggg tatagggatc gcgggttaac       60

gaatcgcgcg tgcgtcgcgt tagtttgcgt cgagtcgtcg tatacggttt cgggagttcg      120

cgtttaggta cgttttttag gttgttaagt agggtgttaa taagtgcgta cgcgcggacg      180

tttacgtagg cgtacgcgtc gtggcgtttt cgggcgtaat tttagttgtt gtgacgggtt      240

agggttcgta gttcgtttgt tagagtttgt gg                                    272


<210>  26
<211>  272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLRA3_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  26
ggaggaagaa gagtagtaat agtcggaaag aggacgtttt tttatttgtt ttaagcgtcg       60

ttcgtcggaa tttagttttt tattttgggt taaaattcga gattcggagc gcggtaaagg      120

gaagcggggg tggggtcgga taattcggtt ttgtcgatta attacgatcg tatagcggga      180

cggagtgata ggttatttac gacggtaggc ggggtttagc gttttatttt ttaataaaat      240

ttaaataaat taaacggcga ttggagcgag gt                                    272


<210>  27
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FBXO39_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  27
gttttttttg tgcgtagaag taagggtaga gttttaaatt tgtagttgtt gttttttttg       60

tgcgtagaag taagggcggg ggcgtgttat ttttattcgg agcgtcggtt gagtttttcg      120

ttttttaacg tttggttagg gtttcgtttt gttttttgta tatgggattt attgattagg      180

taattgtagg tttggggttt tg                                               202


<210>  28
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HTR7_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  28
tttagtttga tttttttagg aaaggcgagc gcgcggggtt gagttgttag tcggttttta       60

gtcggagttt gggacggggc ggttcggttt ttatttgtta atgttgatta cgtatagggt      120

tatgatcgag gtcgtgtagt atatgacgtt tatggcgatg aagatattat agaaaaagtg      180

ttt                                                                    183


<210>  29
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SLCA7_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  29
tgtaagaggt tataaagttt gggcgtagga aatgggtaga gggggtcgag gaarggtcgt       60

agggggtcgg gagagtatcg ggttgttgcg gagaggaatt tgtttgtttg g               111


<210>  30
<211>  194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPR78_BC Methylation Clock (non- CpG cytosines deaminated)

<400>  30
ggtttagaga tagatttatt aggaggacgt ttgaggttcg agtgcggagt ttagcgttgt       60

aggcgtaata aagtagtatt agtgcgttgg atagtagcgt tacggttagt attattatta      120

ggagattcgt tagtagcgtt tcgtcggggt ttatggcgtt agcggttcgt taggtatttt      180

ggggttttta tggt                                                        194


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRX2-fwd

<400>  31
gtatattttg ttaggattgg agt                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRX2-rev

<400>  32
cataaaaccc acatctcttt caa                                               23


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454-fwd (long)

<400>  33
attaggtttt tgttttgtat taagg                                             25


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454-rev (long)

<400>  34
ttctattacc ttccaaacct tttc                                              24


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454-fwd (short)

<400>  35
gggaggttag tttaggggag                                                   20


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZNF454-rev (short)

<400>  36
ttctattacc ttccaaacct ttt                                               23


