                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  TARGETING TRANSFER RNA FOR THE SUPPRESSION OF NONSENSE MUTATIONS 
       IN MESSENGER RNA

<130>  00015-381WO1

<140>  Not yet assigned
<141>  2021-01-08

<150>  US 62/959,087
<151>  2020-01-09

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Random template illustration

<400>  1
uucgauuggu aguucgauug g                                                 21


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Random template illustration

<400>  2

Phe Asp Trp Ser Phe Asp Trp 
1               5           


<210>  3
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ser tRNA

<400>  3
gtagtcgtgg ccgagtggtt aaggcgatgg actagaaatc cattggggtt tccccgcgca       60

ggttcgaatc ctgccgacta cg                                                82


<210>  4
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified tRNA Ser-TAG

<400>  4
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattggggtt tccccgcgca       60

ggttcgaatc ctgccgacta cg                                                82


<210>  5
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-BoxBv1

<400>  5
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattggccct gaaaaagggc       60

cgcgcaggtt cgaatcctgc cgactacg                                          88


<210>  6
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-BoxBv2

<400>  6
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgggccc tgaaaaaggg       60

ccgcgcaggt tcgaatcctg ccgactacg                                         89


<210>  7
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG MS2v2

<400>  7
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattggggac atgaggatca       60

cccatgtccc gcgcaggttc gaatcctgcc gactacg                                97


<210>  8
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-U1Av3

<400>  8
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgagctt atccattgca       60

ctccggataa gctgcgcagg ttcgaatcct gccgactacg                            100


<210>  9
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-Gas5v4

<400>  9
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgggccc agtggtcttt       60

gtagactgcc tgatggccgc gcaggttcga atcctgccga ctacg                      105


<210>  10
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-TARv1

<400>  10
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgggcca gatctgagcc       60

tgggagctct ctggccgcgc aggttcgaat cctgccgact acg                        103


<210>  11
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-SLBPv1

<400>  11
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgccaaa ggctcttctc       60

agagccaccc agcgcaggtt cgaatcctgc cgactacg                               98


<210>  12
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ttRNA Ser-TAG-SLBPv2

<400>  12
gtagtcgtgg ccgagtggtt aaggcgatgg actctaaatc cattgggctc ttctcagagc       60

cgcgcaggtt cgaatcctgc cgactacg                                          88


<210>  13
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cLuc gRNA with MS2 Hairpin

<400>  13
aggacatgag gatcacccat gtcccccaca gctgctatca atacaaggac atgaggatca       60

cccatgtccc                                                              70


<210>  14
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Box B sequence

<400>  14
gcccugaaaa agggc                                                        15


<210>  15
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  BoxB sequence

<400>  15
ggcccugaaa aagggcc                                                      17


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csy4 target coding sequence

<400>  16
gttcactgcc gtataggcag                                                   20


<210>  17
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Csy4 RNA target sequence

<400>  17
guucacugcc guauaggcag cuaagaaa                                          28


<210>  18
<211>  6
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ttRNA anticodon consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is a pyrimidine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  N is a purine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  N is U, A or C

<400>  18
nucann                                                                   6


<210>  19
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ttRNA anticodon consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is a pyrimidine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  N is a purine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  N is U, A or C

<400>  19
nuuuann                                                                  7


<210>  20
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ttRNA anticodon consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is a pyrimidine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  N is a purine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  N is U, A or C

<400>  20
nuucann                                                                  7


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for LambdaN

<400>  21
ggccctgaaa aagggcc                                                      17


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for MCP

<400>  22
gggacatgag gatcacccat gtccc                                             25


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for U1A

<400>  23
agcttatcca ttgcactccg gataagct                                          28


<210>  24
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for GR-DBD

<400>  24
ggcccagtgg tctttgtaga ctgcctgatg gcc                                    33


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for TBP6.7

<400>  25
ggccagatct gagcctggga gctctctggc c                                      31


<210>  26
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for SLBP

<400>  26
ccaaaggctc ttctcagagc caccca                                            26


<210>  27
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide coding motif for SLBP

<400>  27
ggctcttctc agagcc                                                       16


<210>  28
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Ala


<220>
<221>  misc_feature
<222>  (17)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  n is either present or absent and if present n is any RNA base

<400>  28
ggggrwkuag cucardnngg unnagagcgc nyncuuhgca ugnrngagnn nnnnnnnnnn       60

nnnnnnngym sygggwucra yscccrsmwy cucca                                  95


<210>  29
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Arg


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (71)..(72)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (86)..(87)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (89)..(90)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (93)..(94)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<400>  29
nnsynngugg csnaaunngg aunansgcrn yknmcuhckr aknmrnrgnn nnnnnnnnnn       60

nnnnnnnruu nnrgguucga vuccynncnn rsnnr                                  95


<210>  30
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Asn


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (43)..(45)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (91)..(91)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<400>  30
nucncugugg cncaauysgg yunagngcgu nnngcuguua acnnnaagnn nnnnnnnnnn       60

nnnnnnnguu ggugguucra dcccacccag ngang                                  95


<210>  31
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Asp


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (49)..(69)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (91)..(91)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<400>  31
uccncnuuag uauagunngg uknaguauyc ccnccuguca cgngggagnn nnnnnnnnnn       60

nnnnnnnanc csggguucra uucccsgang nggag                                  95


<210>  32
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Cys


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (73)..(73)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (85)..(85)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<220>
<221>  misc_feature
<222>  (88)..(88)
<223>  n is a paired consensus sequence A/T, A/U or G/C

<400>  32
gggggunuag cucagbnngg unnagagcau uugacugcag aucaagagnn nnnnnnnnnn       60

nnnnnnnguc ccnrguucaa aucynggngc ccccu                                  95


<210>  33
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Primate tRNA consensus sequence isotype Gln


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (49)..(67)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (69)..(69)
<223>  n is either present or absent and if present n is any RNA base

<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  n is either present or absent and if present n is any RNA base

<400>  33
ggnyccaugg uguaaunngg uknagcacuc uggacuyuga auccagyrnn nnnnnnnnnn       60

nnnnnnnanu ccgaguucra rucucggugg rnccu                                  95


