                         SEQUENCE LISTING

<110>  DANISCO US INC
 
<120>  METHODS AND COMPOSITIONS FOR ENHANCED ETHANOL PRODUCTION

<130>  NB41362-WO-PCT

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1571
<212>  DNA
<213>  Hypocrea jecorina

<400>  1
atgcactcac gactcctcat cacggccctc ttcctgggcc tcatcgccct ggtctcggcc       60

tcggccatcc ccatccacca gaagcgcggc tccttcaagg tcgagcggag ggccaacccc      120

aacttcaccg gcaatgacgg cctcaaggcc atggccaagg cgtaccgcaa gttcggctgg      180

accatgcccc aggacctcaa ggacgcgctg gcggtgcggg aggcggccgt caaggcccgc      240

cgggctgcgg cggctgtggc tgcgcaggag gctgctgcca agaagaagag cagacgatcg      300

ctgttggatc tcctgggtga gcttggcctg ctcgggggaa acggaggcaa tggatcaaac      360

gatgatgcca atgccaatcc taacggtggc agcggaagac atcaccacgg caatggaaat      420

ggcaacggca acggcaacgg aaatggtcaa ggacaaggcc aaggccaggc tggaggaaac      480

cagactcagc ctgccccggc agcccagccc gcctctggcc aggtcggaag cgtcacaaac      540

acgcccgagg gcaacgacgt cgagttcctg tcccccgtca agatcggcgg ccagacgctc      600

aacctcgact tcgacaccgg ctcctctgac ctctgggtct tcaacacggc catggatccc      660

tcgctgacgg ccggccacac cctgtacgat cccaccaaga gcaagacctt caagcagatc      720

cagggcgcgc agttcctcgt ccagtacggc gacggctcag gcgccgaggg cgtcgtcggc      780

acggacgttg tcgacgttgg cggcgccgtc ttcgacgccc aggccgtcga gattgccacc      840

gccgtcacgc agcagtttgt cgacgaccag cagaacgacg ggctcatggg cctcgccttt      900

tccaagctca acacggtcca gccgcagcag caaaagacgt tcctcgacaa cgtccagagc      960

tccctcgccg agcccgtctt caccgccgac ctcaagaagg gccagcccgg cacgtacacc     1020

tttggcgccg tcgacgcctc cgccttccag ggcgacctga cctgggtcga cgtcgacaac     1080

tcgcagggct tctggcagtt cagcagcgag tcctttgccg tcgacggcgg cgcgacccag     1140

caggccacgg ccggcggcca ggccattgcc gacaccggca ccaccctgct gctggccgac     1200

cccatcatcg tccagggcta ctacgcaaag gtccagggcg cccagaacga tgcccaggcc     1260

ggtggcttta ccgtgccgtg cgatgcccag ctgcccgact tggacctgga tgttggcgga     1320

aagtatgtgg cccgcatcag cggttcggac ctcaactttg cgccggttca gggaaacagt     1380

aagttgctac cgtatcatct ttttactgag agacgacttc acaggactga catttttcga     1440

cagcttgctt tggtggtctt caggcaacga cgcagggcgg cctgggtgtc tatggcgaca     1500

tcttcttcaa gtcgcagttt gtggcgttca acattggcaa caacacgctg ggcctggctc     1560

ctcatgctta g                                                          1571


<210>  2
<211>  430
<212>  PRT
<213>  Hypocrea jecorina

<400>  2

Met His Ser Arg Leu Leu Ile Thr Ala Leu Phe Leu Gly Leu Ile Ala 
1               5                   10                  15      


Leu Val Ser Ala Ser Ala Ile Pro Ile His Gln Lys Arg Gly Ser Phe 
            20                  25                  30          


Lys Val Glu Arg Arg Ala Asn Pro Asn Phe Thr Gly Asn Asp Gly Leu 
        35                  40                  45              


Lys Ala Met Ala Lys Ala Tyr Arg Lys Phe Gly Trp Thr Met Pro Gln 
    50                  55                  60                  


Asp Leu Lys Asp Ala Leu Ala Val Arg Glu Ala Ala Val Lys Ala Arg 
65                  70                  75                  80  


Arg Ala Ala Ala Ala Ala Gly Gly Asn Gln Thr Gln Pro Ala Pro Ala 
                85                  90                  95      


Ala Gln Pro Ala Ser Gly Gln Val Gly Ser Val Thr Asn Thr Pro Glu 
            100                 105                 110         


Gly Asn Asp Val Glu Phe Leu Ser Pro Val Lys Ile Gly Gly Gln Thr 
        115                 120                 125             


Leu Asn Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Asn 
    130                 135                 140                 


Thr Ala Met Asp Pro Ser Leu Thr Ala Gly His Thr Leu Tyr Asp Pro 
145                 150                 155                 160 


Thr Lys Ser Lys Thr Phe Lys Gln Ile Gln Gly Ala Gln Phe Leu Val 
                165                 170                 175     


Gln Tyr Gly Asp Gly Ser Gly Ala Glu Gly Val Val Gly Thr Asp Val 
            180                 185                 190         


Val Asp Val Gly Gly Ala Val Phe Asp Ala Gln Ala Val Glu Ile Ala 
        195                 200                 205             


Thr Ala Val Thr Gln Gln Phe Val Asp Asp Gln Gln Asn Asp Gly Leu 
    210                 215                 220                 


Met Gly Leu Ala Phe Ser Lys Leu Asn Thr Val Gln Pro Gln Gln Gln 
225                 230                 235                 240 


Lys Thr Phe Leu Asp Asn Val Gln Ser Ser Leu Ala Glu Pro Val Phe 
                245                 250                 255     


Thr Ala Asp Leu Lys Lys Gly Gln Pro Gly Thr Tyr Thr Phe Gly Ala 
            260                 265                 270         


Val Asp Ala Ser Ala Phe Gln Gly Asp Leu Thr Trp Val Asp Val Asp 
        275                 280                 285             


Asn Ser Gln Gly Phe Trp Gln Phe Ser Ser Glu Ser Phe Ala Val Asp 
    290                 295                 300                 


Gly Gly Ala Thr Gln Gln Ala Thr Ala Gly Gly Gln Ala Ile Ala Asp 
305                 310                 315                 320 


Thr Gly Thr Thr Leu Leu Leu Ala Asp Pro Ile Ile Val Gln Gly Tyr 
                325                 330                 335     


Tyr Ala Lys Val Gln Gly Ala Gln Asn Asp Ala Gln Ala Gly Gly Phe 
            340                 345                 350         


Thr Val Pro Cys Asp Ala Gln Leu Pro Asp Leu Asp Leu Asp Val Gly 
        355                 360                 365             


Gly Lys Tyr Val Ala Arg Ile Ser Gly Ser Asp Leu Asn Phe Ala Pro 
    370                 375                 380                 


Val Gln Gly Asn Thr Cys Phe Gly Gly Leu Gln Ala Thr Thr Gln Gly 
385                 390                 395                 400 


Gly Leu Gly Val Tyr Gly Asp Ile Phe Phe Lys Ser Gln Phe Val Ala 
                405                 410                 415     


Phe Asn Ile Gly Asn Asn Thr Leu Gly Leu Ala Pro His Ala 
            420                 425                 430 


<210>  3
<211>  2388
<212>  DNA
<213>  Aspergillus niger

<400>  3
atgaagttct tctcgacaat ctgcagtttg acccttgcgg tctctgcgtt ggccttgcca       60

acttctgacc atgttattca tgagaagcga tctggtaccc catctcgatg ggagaagatc      120

agtcgggtca atggtactga gcatgtcttg gtgcgcatcg gtctgacgca gaacaatctt      180

gatcgagcct atgagtatct catgagcgtg tatgttgctt cctcccccct atattgttcc      240

gactgactag tcaggtctga ctccgtgtcg cctaactacg gcaaattctg gactccagaa      300

gaggtgcaca gtaccttcgc gccgtcgaat gagactgtca acgctgtgcg caactggctt      360

attgaatccg gtgtggatga gtctcgcttg gttcacacca aaaaccaagg ctggattgtg      420

tttgatgcaa ccaccaaaga agcggaaaat ctcctccaca ccaagtatta ccactacaca      480

gaccggattt ctggcttcaa gacactcgca gcagaagagt atgtctccca tttattctca      540

gagatatagt atactaacaa gacgaccagg taccgcgtcc cccagaagat ccaacaacac      600

atcgacttca ttaaacccgg cgttcttctc ccattgacct ccaagggacc atccgccaag      660

cacaccaaga aatacaaacc cttgaagcag acatcagtca acgccacgtc tctcaccacg      720

tgcgacgagg tcatcactcc agcttgtgtc gccgctctgt acaagatccc tcacgcaagc      780

ggcaacgtca gtgcaagcaa ctcgcttggg atcttcgaag aaggagacta ctacgcccag      840

gaagatctgg atctcttttt ccgcaacttc accccgtaca ttccgaaggg aacacaccct      900

aagccggcat ttatcgatgg agcctcagcg cccgtgagcg ttgctgatgc tggtgcggag      960

tcggatttgg acttccagct tgcgtatcca attgtttacc ctcagaccat cacgctgtat     1020

cagacagatg actatgacta tgcgagtggg gaggttgaga ctgatggatt ctttaacacc     1080

tttcttgatg ctgttgatgg ggtaagtagc ctctggaatt ttgggattta gctaatgtct     1140

cagtcatact gcacctactg tgcctatggg gaatgcggag acagcccgac tctcgatccc     1200

acttacccag ataactccac cggaggttac aagggacagt tgatgtgcgg tgtttataag     1260

cccacgaatg tcatctccgt ttcttatggt ggccaggagg cagacctccc ggcttactac     1320

cagcagcgac aatgcaatga gtatgccatc cccacctcta tttatacagc ccgaattaac     1380

aacaggaata gattcctcaa gctcggtctc cagggcattt ccatcctctt cgcctctggc     1440

gatgacggcg tcgcagggcc cccaggcgac gactcgacta acgggtgtct gggaaatgga     1500

accatcttca gtcctgcgtt ccccaattcg tatgttttct tctcttgcaa tattcttgca     1560

acaaccacat ctaacttccc tagttgcccc tgggtgacta atgtcggagc caccaaactc     1620

taccccggaa agaccatcgc agacggggaa agcgccgtcg tcgacccggc cggccacccc     1680

tactcggtcg cattctcctc tggtggtggc ttcagcaaca tctatactat tccagactat     1740

caagccgaag cagtagcaga gtaggtgctt ttctatcatc ctatacgcgc aatctaacag     1800

cccccattca gatacttcaa aaagcacaac ccaccctatc cttactacga aggcaacgcc     1860

agcttcggca aaaacggcgg tgtctacaac cgtcttggac gcgggtaccc cgacgtggca     1920

gcgaacggcg acaacatcgc cgagtacaac gcgggagaat tcatacttga gggtggaact     1980

agtgctagta cgttactacc ctactacatc cacccaactc cgttatattt caattactaa     2040

tgacaatgat taaaggtacc ccgatcttct cctccgtgat taaccgcatc atcgagaagc     2100

gaatcgcggc aggaaagggc ccactaggct tcctgaaccc ggttctgtat cggaatgcgt     2160

gggcgttgaa tgatattacg aatgggtcga atccgggttg tggaacggag gggttctata     2220

ctgctcctgg gtatgtattt ccctctcgtt tttttatttc tttattatcg tacttcgata     2280

tatggagcca tgctaacaat cggtgaacaa aatagatggg atcccgtcac cggtctcgga     2340

acgcctaact tcccgaaatt gctagacgtg ttcctgaacc tcccgtaa                  2388


<210>  4
<211>  646
<212>  PRT
<213>  Aspergillus niger

<400>  4

Met Lys Phe Phe Ser Thr Ile Cys Ser Leu Thr Leu Ala Val Ser Ala 
1               5                   10                  15      


Leu Ala Leu Pro Thr Ser Asp His Val Ile His Glu Lys Arg Ser Gly 
            20                  25                  30          


Thr Pro Ser Arg Trp Glu Lys Ile Ser Arg Val Asn Gly Thr Glu His 
        35                  40                  45              


Val Leu Val Arg Ile Gly Leu Thr Gln Asn Asn Leu Asp Arg Ala Tyr 
    50                  55                  60                  


Glu Tyr Leu Met Ser Val Ser Asp Ser Val Ser Pro Asn Tyr Gly Lys 
65                  70                  75                  80  


Phe Trp Thr Pro Glu Glu Val His Ser Thr Phe Ala Pro Ser Asn Glu 
                85                  90                  95      


Thr Val Asn Ala Val Arg Asn Trp Leu Ile Glu Ser Gly Val Asp Glu 
            100                 105                 110         


Ser Arg Leu Val His Thr Lys Asn Gln Gly Trp Ile Val Phe Asp Ala 
        115                 120                 125             


Thr Thr Lys Glu Ala Glu Asn Leu Leu His Thr Lys Tyr Tyr His Tyr 
    130                 135                 140                 


Thr Asp Arg Ile Ser Gly Phe Lys Thr Leu Ala Ala Glu Glu Tyr Arg 
145                 150                 155                 160 


Val Pro Gln Lys Ile Gln Gln His Ile Asp Phe Ile Lys Pro Gly Val 
                165                 170                 175     


Leu Leu Pro Leu Thr Ser Lys Gly Pro Ser Ala Lys His Thr Lys Lys 
            180                 185                 190         


Tyr Lys Pro Leu Lys Gln Thr Ser Val Asn Ala Thr Ser Leu Thr Thr 
        195                 200                 205             


Cys Asp Glu Val Ile Thr Pro Ala Cys Val Ala Ala Leu Tyr Lys Ile 
    210                 215                 220                 


Pro His Ala Ser Gly Asn Val Ser Ala Ser Asn Ser Leu Gly Ile Phe 
225                 230                 235                 240 


Glu Glu Gly Asp Tyr Tyr Ala Gln Glu Asp Leu Asp Leu Phe Phe Arg 
                245                 250                 255     


Asn Phe Thr Pro Tyr Ile Pro Lys Gly Thr His Pro Lys Pro Ala Phe 
            260                 265                 270         


Ile Asp Gly Ala Ser Ala Pro Val Ser Val Ala Asp Ala Gly Ala Glu 
        275                 280                 285             


Ser Asp Leu Asp Phe Gln Leu Ala Tyr Pro Ile Val Tyr Pro Gln Thr 
    290                 295                 300                 


Ile Thr Leu Tyr Gln Thr Asp Asp Tyr Asp Tyr Ala Ser Gly Glu Val 
305                 310                 315                 320 


Glu Thr Asp Gly Phe Phe Asn Thr Phe Leu Asp Ala Val Asp Gly Ser 
                325                 330                 335     


Tyr Cys Thr Tyr Cys Ala Tyr Gly Glu Cys Gly Asp Ser Pro Thr Leu 
            340                 345                 350         


Asp Pro Thr Tyr Pro Asp Asn Ser Thr Gly Gly Tyr Lys Gly Gln Leu 
        355                 360                 365             


Met Cys Gly Val Tyr Lys Pro Thr Asn Val Ile Ser Val Ser Tyr Gly 
    370                 375                 380                 


Gly Gln Glu Ala Asp Leu Pro Ala Tyr Tyr Gln Gln Arg Gln Cys Asn 
385                 390                 395                 400 


Glu Phe Leu Lys Leu Gly Leu Gln Gly Ile Ser Ile Leu Phe Ala Ser 
                405                 410                 415     


Gly Asp Asp Gly Val Ala Gly Pro Pro Gly Asp Asp Ser Thr Asn Gly 
            420                 425                 430         


Cys Leu Gly Asn Gly Thr Ile Phe Ser Pro Ala Phe Pro Asn Ser Cys 
        435                 440                 445             


Pro Trp Val Thr Asn Val Gly Ala Thr Lys Leu Tyr Pro Gly Lys Thr 
    450                 455                 460                 


Ile Ala Asp Gly Glu Ser Ala Val Val Asp Pro Ala Gly His Pro Tyr 
465                 470                 475                 480 


Ser Val Ala Phe Ser Ser Gly Gly Gly Phe Ser Asn Ile Tyr Thr Ile 
                485                 490                 495     


Pro Asp Tyr Gln Ala Glu Ala Val Ala Glu Tyr Phe Lys Lys His Asn 
            500                 505                 510         


Pro Pro Tyr Pro Tyr Tyr Glu Gly Asn Ala Ser Phe Gly Lys Asn Gly 
        515                 520                 525             


Gly Val Tyr Asn Arg Leu Gly Arg Gly Tyr Pro Asp Val Ala Ala Asn 
    530                 535                 540                 


Gly Asp Asn Ile Ala Glu Tyr Asn Ala Gly Glu Phe Ile Leu Glu Gly 
545                 550                 555                 560 


Gly Thr Ser Ala Ser Thr Pro Ile Phe Ser Ser Val Ile Asn Arg Ile 
                565                 570                 575     


Ile Glu Lys Arg Ile Ala Ala Gly Lys Gly Pro Leu Gly Phe Leu Asn 
            580                 585                 590         


Pro Val Leu Tyr Arg Asn Ala Trp Ala Leu Asn Asp Ile Thr Asn Gly 
        595                 600                 605             


Ser Asn Pro Gly Cys Gly Thr Glu Gly Phe Tyr Thr Ala Pro Gly Trp 
    610                 615                 620                 


Asp Pro Val Thr Gly Leu Gly Thr Pro Asn Phe Pro Lys Leu Leu Asp 
625                 630                 635                 640 


Val Phe Leu Asn Leu Pro 
                645     


<210>  5
<211>  1302
<212>  DNA
<213>  Hypocrea jecorina

<400>  5
atgcagacct ttggagcttt tctcgtttcc ttcctcgccg ccagcggcct ggccgcggcc       60

ctccccaccg agggtcagaa gacggcttcc gtcgaggtcc agtacaacaa gaactacgtc      120

ccccacggcc ctactgctct cttcaaggcc aagagaaagt atggcgctcc catcagcgac      180

aacctgaagt ctctcgtggc tgccaggcag gccaagcagg ctctcgccaa gcgccagacc      240

ggctcggcgc ccaaccaccc cagtgacagc gccgattcgg agtacatcac ctccgtctcc      300

atcggcactc cggctcaggt cctccccctg gactttgaca ccggctcctc cgacctgtgg      360

gtctttagct ccgagacgcc caagtcttcg gccaccggcc acgccatcta cacgccctcc      420

aagtcgtcca cctccaagaa ggtgtctggc gccagctggt ccatcagcta cggcgacggc      480

agcagctcca gcggcgatgt ctacaccgac aaggtcacca tcggaggctt cagcgtcaac      540

acccagggcg tcgagtctgc cacccgcgtg tccaccgagt tcgtccagga cacggtcatc      600

tctggcctcg tcggccttgc ctttgacagc ggcaaccagg tcaggccgca cccgcagaag      660

acgtggttct ccaacgccgc cagcagcctg gctgagcccc ttttcactgc cgacctgagg      720

cacggacaga gtaagtagac actcactgga attcgttcct ttcccgatca tcatgaaagc      780

aagtagactg actgaaccaa acaactagac ggcagctaca actttggcta catcgacacc      840

agcgtcgcca agggccccgt tgcctacacc cccgttgaca acagccaggg cttctgggag      900

ttcactgcct cgggctactc tgtcggcggc ggcaagctca accgcaactc catcgacggc      960

attgccgaca ccggcaccac cctgctcctc ctcgacgaca acgtcgtcga tgcctactac     1020

gccaacgtcc agtcggccca gtacgacaac cagcaggagg gtgtcgtctt cgactgcgac     1080

gaggacctcc cttcgttcag cttcggtgtt ggaagctcca ccatcaccat ccctggcgat     1140

ctgctgaacc tgactcccct cgaggagggc agctccacct gcttcggtgg cctccagagc     1200

agctccggca ttggcatcaa catctttggt gacgttgccc tcaaggctgc cctggttgtc     1260

tttgacctcg gcaacgagcg cctgggctgg gctcagaaat aa                        1302


<210>  6
<211>  407
<212>  PRT
<213>  Hypocrea jecorina

<400>  6

Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly 
1               5                   10                  15      


Leu Ala Ala Ala Leu Pro Thr Glu Gly Gln Lys Thr Ala Ser Val Glu 
            20                  25                  30          


Val Gln Tyr Asn Lys Asn Tyr Val Pro His Gly Pro Thr Ala Leu Phe 
        35                  40                  45              


Lys Ala Lys Arg Lys Tyr Gly Ala Pro Ile Ser Asp Asn Leu Lys Ser 
    50                  55                  60                  


Leu Val Ala Ala Arg Gln Ala Lys Gln Ala Leu Ala Lys Arg Gln Thr 
65                  70                  75                  80  


Gly Ser Ala Pro Asn His Pro Ser Asp Ser Ala Asp Ser Glu Tyr Ile 
                85                  90                  95      


Thr Ser Val Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe 
            100                 105                 110         


Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Lys 
        115                 120                 125             


Ser Ser Ala Thr Gly His Ala Ile Tyr Thr Pro Ser Lys Ser Ser Thr 
    130                 135                 140                 


Ser Lys Lys Val Ser Gly Ala Ser Trp Ser Ile Ser Tyr Gly Asp Gly 
145                 150                 155                 160 


Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Lys Val Thr Ile Gly Gly 
                165                 170                 175     


Phe Ser Val Asn Thr Gln Gly Val Glu Ser Ala Thr Arg Val Ser Thr 
            180                 185                 190         


Glu Phe Val Gln Asp Thr Val Ile Ser Gly Leu Val Gly Leu Ala Phe 
        195                 200                 205             


Asp Ser Gly Asn Gln Val Arg Pro His Pro Gln Lys Thr Trp Phe Ser 
    210                 215                 220                 


Asn Ala Ala Ser Ser Leu Ala Glu Pro Leu Phe Thr Ala Asp Leu Arg 
225                 230                 235                 240 


His Gly Gln Asn Gly Ser Tyr Asn Phe Gly Tyr Ile Asp Thr Ser Val 
                245                 250                 255     


Ala Lys Gly Pro Val Ala Tyr Thr Pro Val Asp Asn Ser Gln Gly Phe 
            260                 265                 270         


Trp Glu Phe Thr Ala Ser Gly Tyr Ser Val Gly Gly Gly Lys Leu Asn 
        275                 280                 285             


Arg Asn Ser Ile Asp Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu 
    290                 295                 300                 


Leu Asp Asp Asn Val Val Asp Ala Tyr Tyr Ala Asn Val Gln Ser Ala 
305                 310                 315                 320 


Gln Tyr Asp Asn Gln Gln Glu Gly Val Val Phe Asp Cys Asp Glu Asp 
                325                 330                 335     


Leu Pro Ser Phe Ser Phe Gly Val Gly Ser Ser Thr Ile Thr Ile Pro 
            340                 345                 350         


Gly Asp Leu Leu Asn Leu Thr Pro Leu Glu Glu Gly Ser Ser Thr Cys 
        355                 360                 365             


Phe Gly Gly Leu Gln Ser Ser Ser Gly Ile Gly Ile Asn Ile Phe Gly 
    370                 375                 380                 


Asp Val Ala Leu Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Glu 
385                 390                 395                 400 


Arg Leu Gly Trp Ala Gln Lys 
                405         


