               SEQUENCE LISTING

<110> Quanta Therapeutics, Inc. 

<120> COMPOSITIONS AND METHODS FOR IDENTIFICATION OF MODULATORS OF RAS PROTEIN COMPLEXES

<130> 55172-701.601

<140> PCT/US2021/012356
<141> 2021-01-06

<150> US63/068,120
<151> 2020-08-20

<150> US62/984,761
<151> 2020-03-03

<150> US62/957,786
<151> 2020-01-06

<160> 5

<170> BiSSAP 1.3.6

<210> 1
<211> 186
<212> PRT
<213> Artificial Sequence

<220> 
<223> Ras protein

<400> 1
Gly Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly 
1               5                   10                  15      
Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu 
            20                  25                  30          
Tyr Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp 
        35                  40                  45              
Gly Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu 
    50                  55                  60                  
Tyr Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu 
65                  70                  75                  80  
Cys Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His 
                85                  90                  95      
Tyr Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met 
            100                 105                 110         
Val Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr 
        115                 120                 125             
Lys Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu 
    130                 135                 140                 
Thr Ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala Phe Tyr Thr Leu 
145                 150                 155                 160 
Val Arg Glu Ile Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys 
                165                 170                 175     
Lys Lys Lys Lys Lys Ser Lys Thr Lys Cys 
            180                 185     

<210> 2
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Raf protein

<400> 2
Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn Lys Gln Arg 
1               5                   10                  15      
Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp Cys Leu Met 
            20                  25                  30          
Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys Ala Val Phe 
        35                  40                  45              
Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu Asp Trp Asn 
    50                  55                  60                  
Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val Asp Phe Leu 
65                  70                  75                  80  
Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys Thr Phe Leu 
                85                  90                  95      
Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu Asn Gly Phe 
            100                 105                 110         
Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys Ser Thr Lys 
        115                 120                 125             
Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln 
    130                 135                 140     

<210> 3
<211> 648
<212> PRT
<213> Artificial Sequence

<220> 
<223> Full-length c-RAF protein [P04049.1]

<400> 3
Met Glu His Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly 
1               5                   10                  15      
Phe Lys Asp Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile 
            20                  25                  30          
Val Gln Gln Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu 
        35                  40                  45              
Thr Asp Pro Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn 
    50                  55                  60                  
Lys Gln Arg Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp 
65                  70                  75                  80  
Cys Leu Met Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys 
                85                  90                  95      
Ala Val Phe Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu 
            100                 105                 110         
Asp Trp Asn Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val 
        115                 120                 125             
Asp Phe Leu Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys 
    130                 135                 140                 
Thr Phe Leu Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu 
145                 150                 155                 160 
Asn Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys 
                165                 170                 175     
Ser Thr Lys Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln 
            180                 185                 190         
Leu Leu Leu Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala 
        195                 200                 205             
Leu Pro Ser Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met 
    210                 215                 220                 
Pro Val Ser Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe 
225                 230                 235                 240 
Asn Thr Ser Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg 
                245                 250                 255     
Ser Thr Ser Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val 
            260                 265                 270         
Asp Ser Arg Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala 
        275                 280                 285             
Ser Pro Ser Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly 
    290                 295                 300                 
Trp Ser Gln Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro 
305                 310                 315                 320 
Val Ser Gly Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg 
                325                 330                 335     
Asp Ser Ser Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser 
            340                 345                 350         
Thr Arg Ile Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp 
        355                 360                 365             
His Gly Asp Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro 
    370                 375                 380                 
Glu Gln Phe Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr 
385                 390                 395                 400 
Arg His Val Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn 
                405                 410                 415     
Leu Ala Ile Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His 
            420                 425                 430         
Leu His Val Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile 
        435                 440                 445             
Ala Arg Gln Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile 
    450                 455                 460                 
Ile His Arg Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu 
465                 470                 475                 480 
Thr Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp 
                485                 490                 495     
Ser Gly Ser Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met 
            500                 505                 510         
Ala Pro Glu Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln 
        515                 520                 525             
Ser Asp Val Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly 
    530                 535                 540                 
Glu Leu Pro Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met 
545                 550                 555                 560 
Val Gly Arg Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn 
                565                 570                 575     
Cys Pro Lys Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val 
            580                 585                 590         
Lys Glu Glu Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu 
        595                 600                 605             
Leu Gln His Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser 
    610                 615                 620                 
Leu His Arg Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr 
625                 630                 635                 640 
Thr Ser Pro Arg Leu Pro Val Phe 
                645             

<210> 4
<211> 766
<212> PRT
<213> Artificial Sequence

<220> 
<223> Full-length b-RAF protein [AAA35609.2]

<400> 4
Met Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln 
1               5                   10                  15      
Ala Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly 
            20                  25                  30          
Ala Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp 
        35                  40                  45              
Asn Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu 
    50                  55                  60                  
Leu Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu 
65                  70                  75                  80  
Ala Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu 
                85                  90                  95      
Gln Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser 
            100                 105                 110         
Ser Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu 
        115                 120                 125             
Ser Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val 
    130                 135                 140                 
Ala Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe 
145                 150                 155                 160 
Leu Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr 
                165                 170                 175     
Val Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro 
            180                 185                 190         
Glu Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile 
        195                 200                 205             
Gly Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val 
    210                 215                 220                 
Glu Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys 
225                 230                 235                 240 
Thr Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe 
                245                 250                 255     
Gln Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys 
            260                 265                 270         
Ser Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu 
        275                 280                 285             
Leu Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu 
    290                 295                 300                 
Ala Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala 
305                 310                 315                 320 
Pro Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro 
                325                 330                 335     
Ser Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp 
            340                 345                 350         
His Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn 
        355                 360                 365             
Val His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg 
    370                 375                 380                 
Asp Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala 
385                 390                 395                 400 
Thr Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu 
                405                 410                 415     
Gln Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser 
            420                 425                 430         
Glu Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp 
        435                 440                 445             
Asp Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly 
    450                 455                 460                 
Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val 
465                 470                 475                 480 
Ala Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln 
                485                 490                 495     
Ala Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn 
            500                 505                 510         
Ile Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val 
        515                 520                 525             
Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile 
    530                 535                 540                 
Glu Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr 
545                 550                 555                 560 
Ala Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp 
                565                 570                 575     
Leu Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile 
            580                 585                 590         
Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His 
        595                 600                 605             
Gln Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val 
    610                 615                 620                 
Ile Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr 
625                 630                 635                 640 
Ala Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr 
                645                 650                 655     
Ser Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly 
            660                 665                 670         
Tyr Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala 
        675                 680                 685             
Met Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg 
    690                 695                 700                 
Pro Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser 
705                 710                 715                 720 
Leu Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala 
                725                 730                 735     
Gly Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys 
            740                 745                 750         
Thr Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His 
        755                 760                 765     

<210> 5
<211> 606
<212> PRT
<213> Artificial Sequence

<220> 
<223> Full-length a-RAF protein [P10398.2]

<400> 5
Met Glu Pro Pro Arg Gly Pro Pro Ala Asn Gly Ala Glu Pro Ser Arg 
1               5                   10                  15      
Ala Val Gly Thr Val Lys Val Tyr Leu Pro Asn Lys Gln Arg Thr Val 
            20                  25                  30          
Val Thr Val Arg Asp Gly Met Ser Val Tyr Asp Ser Leu Asp Lys Ala 
        35                  40                  45              
Leu Lys Val Arg Gly Leu Asn Gln Asp Cys Cys Val Val Tyr Arg Leu 
    50                  55                  60                  
Ile Lys Gly Arg Lys Thr Val Thr Ala Trp Asp Thr Ala Ile Ala Pro 
65                  70                  75                  80  
Leu Asp Gly Glu Glu Leu Ile Val Glu Val Leu Glu Asp Val Pro Leu 
                85                  90                  95      
Thr Met His Asn Phe Val Arg Lys Thr Phe Phe Ser Leu Ala Phe Cys 
            100                 105                 110         
Asp Phe Cys Leu Lys Phe Leu Phe His Gly Phe Arg Cys Gln Thr Cys 
        115                 120                 125             
Gly Tyr Lys Phe His Gln His Cys Ser Ser Lys Val Pro Thr Val Cys 
    130                 135                 140                 
Val Asp Met Ser Thr Asn Arg Gln Gln Phe Tyr His Ser Val Gln Asp 
145                 150                 155                 160 
Leu Ser Gly Gly Ser Arg Gln His Glu Ala Pro Ser Asn Arg Pro Leu 
                165                 170                 175     
Asn Glu Leu Leu Thr Pro Gln Gly Pro Ser Pro Arg Thr Gln His Cys 
            180                 185                 190         
Asp Pro Glu His Phe Pro Phe Pro Ala Pro Ala Asn Ala Pro Leu Gln 
        195                 200                 205             
Arg Ile Arg Ser Thr Ser Thr Pro Asn Val His Met Val Ser Thr Thr 
    210                 215                 220                 
Ala Pro Met Asp Ser Asn Leu Ile Gln Leu Thr Gly Gln Ser Phe Ser 
225                 230                 235                 240 
Thr Asp Ala Ala Gly Ser Arg Gly Gly Ser Asp Gly Thr Pro Arg Gly 
                245                 250                 255     
Ser Pro Ser Pro Ala Ser Val Ser Ser Gly Arg Lys Ser Pro His Ser 
            260                 265                 270         
Lys Ser Pro Ala Glu Gln Arg Glu Arg Lys Ser Leu Ala Asp Asp Lys 
        275                 280                 285             
Lys Lys Val Lys Asn Leu Gly Tyr Arg Asp Ser Gly Tyr Tyr Trp Glu 
    290                 295                 300                 
Val Pro Pro Ser Glu Val Gln Leu Leu Lys Arg Ile Gly Thr Gly Ser 
305                 310                 315                 320 
Phe Gly Thr Val Phe Arg Gly Arg Trp His Gly Asp Val Ala Val Lys 
                325                 330                 335     
Val Leu Lys Val Ser Gln Pro Thr Ala Glu Gln Ala Gln Ala Phe Lys 
            340                 345                 350         
Asn Glu Met Gln Val Leu Arg Lys Thr Arg His Val Asn Ile Leu Leu 
        355                 360                 365             
Phe Met Gly Phe Met Thr Arg Pro Gly Phe Ala Ile Ile Thr Gln Trp 
    370                 375                 380                 
Cys Glu Gly Ser Ser Leu Tyr His His Leu His Val Ala Asp Thr Arg 
385                 390                 395                 400 
Phe Asp Met Val Gln Leu Ile Asp Val Ala Arg Gln Thr Ala Gln Gly 
                405                 410                 415     
Met Asp Tyr Leu His Ala Lys Asn Ile Ile His Arg Asp Leu Lys Ser 
            420                 425                 430         
Asn Asn Ile Phe Leu His Glu Gly Leu Thr Val Lys Ile Gly Asp Phe 
        435                 440                 445             
Gly Leu Ala Thr Val Lys Thr Arg Trp Ser Gly Ala Gln Pro Leu Glu 
    450                 455                 460                 
Gln Pro Ser Gly Ser Val Leu Trp Met Ala Ala Glu Val Ile Arg Met 
465                 470                 475                 480 
Gln Asp Pro Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr Ala Tyr Gly 
                485                 490                 495     
Val Val Leu Tyr Glu Leu Met Thr Gly Ser Leu Pro Tyr Ser His Ile 
            500                 505                 510         
Gly Cys Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly Tyr Leu Ser 
        515                 520                 525             
Pro Asp Leu Ser Lys Ile Ser Ser Asn Cys Pro Lys Ala Met Arg Arg 
    530                 535                 540                 
Leu Leu Ser Asp Cys Leu Lys Phe Gln Arg Glu Glu Arg Pro Leu Phe 
545                 550                 555                 560 
Pro Gln Ile Leu Ala Thr Ile Glu Leu Leu Gln Arg Ser Leu Pro Lys 
                565                 570                 575     
Ile Glu Arg Ser Ala Ser Glu Pro Ser Leu His Arg Thr Gln Ala Asp 
            580                 585                 590         
Glu Leu Pro Ala Cys Leu Leu Ser Ala Ala Arg Leu Val Pro 
        595                 600                 605     

