                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  IN SITU RNA ANALYSIS USING PROBE PAIR LIGATION

<130>  P16123-02

<150>  62/956,691
<151>  2020-01-03

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' acceptor probe

<400>  1
agatcggaag agcacac                                                      17


<210>  2
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' phosphorylated donor probe

<400>  2
ggagctgtcg ttcactc                                                      17


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A first detection probe

<400>  3
caagtatgca gcgcgattga ccgtctcgtt                                        30


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A second detection probe

<400>  4
cgcaacgctt gggacggttc caatcggatc                                        30


<210>  5
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A third detection probe

<400>  5
acaaatccga ccagatcgga cgatcatggg                                        30


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A fourth detection probe

<400>  6
caagtatgca gcgcgattga ccgtctcgtt                                        30


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' acceptor probe that specifically hybridizes GAPDH target


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  3' terminal ribonucleotide

<400>  7
ttgagcacag ggtactttat                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' phosphorylated donor probe that specifically hybridizes GAPDH 
       target

<400>  8
actggtctca agtcagtgta                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' acceptor probe that specifically hybridizes beta-actin target


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  3' terminal ribonucleotide

<400>  9
aaggtgtgca cttttattca                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' phosphorylated probe that specifically hybridizes beta-actin 
       target

<400>  10
actggtctca agtcagtgta                                                   20


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bridge oligo primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  nucleotides that contain a phophorothioate bond substitution, 
       which renders them less susceptible to degradation by nucleases

<400>  11
agtgaacgac agctccgtgt gctcttccga tc                                     32


