                         SEQUENCE LISTING

<110>  Biosion Inc.
 
<120>  ANTIBODIES BINDING BCMA AND USES THEREOF

<130>  55532 00026

<150>  US62/956,642
<151>  2020-01-03

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1 for mouse, chimeric and humanized B1H2

<400>  1

Asn Tyr Val Met His 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2 for mouse, chimeric and humanized B1H2

<400>  2

Phe Ile Ile Pro Phe Asn Asp Gly Thr Lys Tyr Asn Glu His Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3 for mouse, chimeric and humanized B1H2

<400>  3

Tyr Asp Phe Glu Gly Tyr Phe Asp Val 
1               5                   


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1 for mouse, chimeric and humanized B1H2

<400>  4

Ser Ala Ser Gln Asp Ile Thr Asn Phe Leu Asn 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2 for mouse, chimeric and humanized B1H2

<400>  5

Ser Thr Ser Arg Leu His Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3 for mouse, chimeric and humanized B1H2

<400>  6

Gln Gln Tyr Ser Asn Leu Pro Trp Thr 
1               5                   


<210>  7
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for mouse and chimeric B1H2

<400>  7

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Leu Thr Asn Tyr 
            20                  25                  30          


Val Met His Trp Met Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Phe Ile Ile Pro Phe Asn Asp Gly Thr Lys Tyr Asn Glu His Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Val Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Asp Phe Glu Gly Tyr Phe Asp Val Trp Gly Ala Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser 
        115             


<210>  8
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huB1H2-V1, huB1H2-V7, huB1H2-V13, and huB1H2-V19

<400>  8

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Leu Thr Asn Tyr 
            20                  25                  30          


Val Met His Trp Met Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Phe Ile Ile Pro Phe Asn Asp Gly Thr Lys Tyr Asn Glu His Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ser Asp Lys Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Asp Phe Glu Gly Tyr Phe Asp Val Trp Gly Ala Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  9
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huB1H2-V2 to huB1H2-V6, huB1H2-V8 to huB1H2-V12, 
       huB1H2-V14 to huB1H2-V18, huB1H2-V20 to huB1H2-V24, and 
       huB1H2-V33


<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be Val or Met

<220>
<221>  misc_feature
<222>  (72)..(72)
<223>  Xaa can be Arg or Ser

<220>
<221>  misc_feature
<222>  (74)..(74)
<223>  Xaa can be Thr or Lys

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Leu Thr Asn Tyr 
            20                  25                  30          


Val Met His Trp Xaa Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Phe Ile Ile Pro Phe Asn Asp Gly Thr Lys Tyr Asn Glu His Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Xaa Asp Xaa Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Asp Phe Glu Gly Tyr Phe Asp Val Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  10
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for mouse and chimeric B1H2

<400>  10

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Asp Ile Thr Asn Phe 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Asn Leu Glu Ile Lys 
            100                 105         


<210>  11
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huB1H2-V1 to huB1H2-V24 and huB1H2-V33


<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be Thr or Ala

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa can be Val or Pro

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be Tyr or Phe

<220>
<221>  misc_feature
<222>  (73)..(73)
<223>  Xaa can be Phe or Leu

<400>  11

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Thr Asn Phe 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Xaa Xaa Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Xaa Thr Xaa Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  12
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain constant region for chimeric and humanized antibodies

<400>  12

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  13
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain constant region for chimeric and humanized antibodies

<400>  13

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  14
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of BCMA BM1

<400>  14

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Thr Tyr Arg Gly His Ser Asp Thr Tyr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Ala Ile Tyr Asp Gly Tyr Asp Val Leu Asp Asn Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215                 220                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
            260                 265                 270         


Glu Asp Pro Glu Val Lys Glu Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Pro Gly Lys 
    450     


<210>  15
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain of BCMA BM1

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Asn Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Arg Lys Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  16
<211>  184
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Leu Gln Met Ala Gly Gln Cys Ser Gln Asn Glu Tyr Phe Asp Ser 
1               5                   10                  15      


Leu Leu His Ala Cys Ile Pro Cys Gln Leu Arg Cys Ser Ser Asn Thr 
            20                  25                  30          


Pro Pro Leu Thr Cys Gln Arg Tyr Cys Asn Ala Ser Val Thr Asn Ser 
        35                  40                  45              


Val Lys Gly Thr Asn Ala Ile Leu Trp Thr Cys Leu Gly Leu Ser Leu 
    50                  55                  60                  


Ile Ile Ser Leu Ala Val Phe Val Leu Met Phe Leu Leu Arg Lys Ile 
65                  70                  75                  80  


Asn Ser Glu Pro Leu Lys Asp Glu Phe Lys Asn Thr Gly Ser Gly Leu 
                85                  90                  95      


Leu Gly Met Ala Asn Ile Asp Leu Glu Lys Ser Arg Thr Gly Asp Glu 
            100                 105                 110         


Ile Ile Leu Pro Arg Gly Leu Glu Tyr Thr Val Glu Glu Cys Thr Cys 
        115                 120                 125             


Glu Asp Cys Ile Lys Ser Lys Pro Lys Val Asp Ser Asp His Cys Phe 
    130                 135                 140                 


Pro Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val Thr Thr Lys 
145                 150                 155                 160 


Thr Asn Asp Tyr Cys Lys Ser Leu Pro Ala Ala Leu Ser Ala Thr Glu 
                165                 170                 175     


Ile Glu Lys Ser Ile Ser Ala Arg 
            180                 


<210>  17
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for mouse B1H2

<400>  17
gaggtccagt tgcagcagtc tggacctgag ctggtaaagc ctggggcttc agtgaagatg       60

tcctgcaagg cttctggata cacattaact aactatgtta tgcactggat gaaacagaag      120

cctgggcagg gccttgagtg gattggattt attattcctt tcaatgatgg tactaaatac      180

aatgagcact tcaaaggcaa ggccacactg acttcagaca aatcctccaa cacagcctac      240

atggttctca gcagcctgac ctctgaggac tctgcggtct attactgtgc aaggtatgat      300

ttcgagggtt acttcgatgt ctggggcgca gggaccacgg tcaccgtctc ctca            354


<210>  18
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for huB1H2-V4, huB1H2-V10, huB1H2-V16, huB1H2-V22, and 
       huB1H2-V33

<400>  18
caggtgcagc tggtgcagag cggcgccgag gtgaagaagc ctggcgcctc cgtgaaggtg       60

agctgcaagg ccagcggcta cacactgaca aattacgtga tgcactgggt gagacaggcc      120

cctggccagg gcctggagtg gatcggattc atcatcccct tcaacgatgg caccaagtac      180

aatgagcact tcaagggcag agtgaccatc accagcgata cctccatctc caccgcctac      240

atgcagctgt ccagcctgag gtccgaggat accgccgtgt actactgcgc caggtacgac      300

ttcgagggct acttcgacgt gtggggccag ggcaccctgg tgaccgtgtc cagc            354


<210>  19
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for mouse B1H2

<400>  19
gatatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc       60

atcagttgca gtgcaagtca ggacattacc aattttttaa actggtatca gcagaaacca      120

gatggaactg ttaaactcct gatctattcc acatcaagat tacactcagg agtcccatca      180

aggttcagtg gcagtgggtc tgggacagat tattctctca ccatcagcaa cctggaacct      240

gaagatattg ccacttacta ttgtcagcag tatagtaacc ttccgtggac gttcggtggc      300

ggcaccaacc tggaaatcaa a                                                321


<210>  20
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for huB1H2-V33

<400>  20
gacatccaga tgacacagag cccctccagc ctgagcgcca gcgtgggaga tagggtgacc       60

atcacctgta gcgcctccca ggatatcacc aacttcctga attggtatca acagaagccc      120

ggcaaggccc ccaagctgct gatctactcc acctccagac tgcacagcgg cgtgccttcc      180

aggttcagcg gctccggctc cggcacagac ttcaccttca ccatctccag cctgcagcct      240

gaggatatcg ccacctacta ctgccagcag tacagcaatc tgccctggac cttcggccag      300

ggcaccaagg tggagatcaa g                                                321


<210>  21
<211>  993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Heavy chain constant region for chimeric and humanized antibodies

<400>  21
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg       60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg      480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac      540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc      660

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag      720

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc      780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg      840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg      900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg      960

cagaagagcc tctccctgtc tccgggtaaa tga                                   993


<210>  22
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Light chain constant region for chimeric and humanized antibodies

<400>  22
cgtacggtgg cggcgccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct       60

ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag      120

tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac      180

agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag      240

aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag      300

agcttcaaca ggggagagtg ttga                                             324


<210>  23
<211>  594
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant protein DT3C

<400>  23

Met Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Lys His Ile Asp Glu Ile Leu Ala Ala Leu Pro Lys Thr Asp Thr 
385                 390                 395                 400 


Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr 
                405                 410                 415     


Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala 
            420                 425                 430         


Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys 
        435                 440                 445             


Thr Phe Thr Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu 
    450                 455                 460                 


Thr Pro Ala Val Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu 
465                 470                 475                 480 


Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys 
                485                 490                 495     


Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr 
            500                 505                 510         


Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu Val 
        515                 520                 525             


Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu Val 
    530                 535                 540                 


Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Lys Ala Val Asp 
545                 550                 555                 560 


Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr Ala Asn Asp Asn Gly 
                565                 570                 575     


Val Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val 
            580                 585                 590         


Thr Glu 
        


<210>  24
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for chimeric B1H2

<400>  24
gaggtgcagc tgcagcagag cggccctgag ctggtgaagc ccggcgcttc cgtgaagatg       60

agctgcaagg ccagcggcta cacactgaca aattacgtga tgcactggat gaagcagaag      120

cccggccagg gcctggagtg gatcggcttc atcatccctt ttaacgacgg caccaagtac      180

aacgagcact ttaagggcaa ggccacactg acatccgaca agagcagcaa taccgcctac      240

atggtgctgt ccagcctgac cagcgaggac tccgccgtgt actactgtgc caggtacgat      300

tttgagggct acttcgacgt gtggggcgcc ggcaccacag tgaccgtgag cagc            354


<210>  25
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for chimeric B1H2

<400>  25
gacatccaga tgacccagac aacaagcagc ctgagcgcct ccctgggcga cagggtgaca       60

atctcctgca gcgccagcca ggacatcaca aacttcctga actggtatca acagaagccc      120

gacggcacag tgaagctgct gatctactcc acatccagac tgcactccgg cgtgccctcc      180

aggttctccg gctccggatc cggcacagat tactccctga ccatcagcaa cctggagcct      240

gaggacatcg ccacctacta ctgccagcag tacagcaatc tgccttggac ctttggcggc      300

ggcaccaatc tggagatcaa g                                                321


