
                                SEQUENCE LISTING

<110> Blobel, Gerd
      Peslak, Scott
      Shi, Junwei

<120> COMPOSITIONS AND METHODS FOR HEMOGLOBIN
  PRODUCTION

<130> 3460-P06747WO00

<150> 62/952,653          
<151> 2019-12-23  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 305
<212> PRT
<213> Homo sapiens

<400> 1
Met Ala Pro Leu Asp Leu Asp Lys Tyr Val Glu Ile Ala Arg Leu Cys
 1               5                  10                  15      
Lys Tyr Leu Pro Glu Asn Asp Leu Lys Arg Leu Cys Asp Tyr Val Cys
            20                  25                  30          
Asp Leu Leu Leu Glu Glu Ser Asn Val Gln Pro Val Ser Thr Pro Val
        35                  40                  45              
Thr Val Cys Gly Asp Ile His Gly Gln Phe Tyr Asp Leu Cys Glu Leu
    50                  55                  60                  
Phe Arg Thr Gly Gly Gln Val Pro Asp Thr Asn Tyr Ile Phe Met Gly
65                  70                  75                  80  
Asp Phe Val Asp Arg Gly Tyr Tyr Ser Leu Glu Thr Phe Thr Tyr Leu
                85                  90                  95      
Leu Ala Leu Lys Ala Lys Trp Pro Asp Arg Ile Thr Leu Leu Arg Gly
            100                 105                 110         
Asn His Glu Ser Arg Gln Ile Thr Gln Val Tyr Gly Phe Tyr Asp Glu
        115                 120                 125             
Cys Gln Thr Lys Tyr Gly Asn Ala Asn Ala Trp Arg Tyr Cys Thr Lys
    130                 135                 140                 
Val Phe Asp Met Leu Thr Val Ala Ala Leu Ile Asp Glu Gln Ile Leu
145                 150                 155                 160 
Cys Val His Gly Gly Leu Ser Pro Asp Ile Lys Thr Leu Asp Gln Ile
                165                 170                 175     
Arg Thr Ile Glu Arg Asn Gln Glu Ile Pro His Lys Gly Ala Phe Cys
            180                 185                 190         
Asp Leu Val Trp Ser Asp Pro Glu Asp Val Asp Thr Trp Ala Ile Ser
        195                 200                 205             
Pro Arg Gly Ala Gly Trp Leu Phe Gly Ala Lys Val Thr Asn Glu Phe
    210                 215                 220                 
Val His Ile Asn Asn Leu Lys Leu Ile Cys Arg Ala His Gln Leu Val
225                 230                 235                 240 
His Glu Gly Tyr Lys Phe Met Phe Asp Glu Lys Leu Val Thr Val Trp
                245                 250                 255     
Ser Ala Pro Asn Tyr Cys Tyr Arg Cys Gly Asn Ile Ala Ser Ile Met
            260                 265                 270         
Val Phe Lys Asp Val Asn Thr Arg Glu Pro Lys Leu Phe Arg Ala Val
        275                 280                 285             
Pro Asp Ser Glu Arg Val Ile Pro Pro Arg Thr Thr Thr Pro Tyr Phe
    290                 295                 300                 
Leu
305 


<210> 2
<211> 918
<212> DNA
<213> Homo sapiens

<400> 2
atggcgccgc tagacctgga caagtatgtg gaaatagcgc ggctgtgcaa gtacctgcca 60
gagaacgacc tgaagcggct atgtgactac gtttgtgacc tcctcttaga agagtcaaat 120
gttcagccag tatcaacacc agtaacagtg tgtggagata tccatggaca gttttatgac 180
ctttgtgaac tgttcagaac tggaggtcag gttcctgaca caaactacat atttatgggt 240
gattttgtag acagaggtta ctatagtttg gagaccttca cttaccttct tgcattaaag 300
gctaaatggc ctgatcgtat tacacttttg cgaggaaatc atgagagtag acagataaca 360
caggtctatg gattttatga tgagtgccaa accaaatatg gaaatgctaa tgcctggaga 420
tactgtacca aagtttttga catgctcaca gtagcagctt taatagatga gcagattttg 480
tgtgtccatg gtggtttatc tcctgatatc aaaacactgg atcaaattcg aaccatcgaa 540
cggaatcagg aaattcctca taaaggagca ttttgtgatc tggtttggtc agatcctgaa 600
gatgtggata cctgggctat cagtccccga ggagcaggtt ggctttttgg agcaaaggtc 660
acaaatgagt ttgttcatat caacaactta aaactcatct gcagagcaca tcaactagtg 720
cacgaaggct ataaatttat gtttgatgag aagctggtga cagtatggtc tgctcctaat 780
tactgctatc gttgtggaaa tattgcttcg atcatggtct tcaaagatgt aaatacaaga 840
gaaccaaagt tattccgggc agttccagat tcagaacgtg ttattcctcc cagaacgaca 900
acgccatatt tcctttga                                               918

<210> 3
<211> 4367
<212> DNA
<213> Homo sapiens

<400> 3
gaagcgggct ctcttggggc ctgcagtcgg ggttcagcca gagggggcgc cgggcgtacc 60
gacgtagcgg ggagaaacgg gtcgcgcttt aacggcgctg cgcaggcgcc actcacgggc 120
cggacgtgac gcagggaaag ttccggcttc ggcctccgcc gctgccgccg ccgctgctac 180
agccgccgcc gccgctgttg ccgcggcttg ttattcttaa aatggcgccg ctagacctgg 240
acaagtatgt ggaaatagcg cggctgtgca agtacctgcc agagaacgac ctgaaggtga 300
gccctatttg cgggctcgcc ccttccggct gtggggcgcc cgccggccgg cccttccttt 360
ctccaggacc cccgccggta tttcattttc ttcgttttct aaaggagcgg ctatgtgact 420
acgtttgtga cctcctctta gaagagtcaa atgttcagcc agtatcaaca ccagtaacag 480
tgtgtggaga tatccatgga cagttttatg acctttgtga actgttcaga actggaggtc 540
aggttcctga cacaaactac atatttatgg gtgattttgt agacagaggt tactatagtt 600
tggagacctt cacttacctt cttgcattaa aggctaaatg gcctgatcgt attacacttt 660
tgcgaggaaa tcatgagagt agacagataa cacaggtcta tggattttat gatgagtgcc 720
aaaccaaata tggaaatgct aatgcctgga gatactgtac caaagttttt gacatgctca 780
cagtagcagc tttaatagat gagcagattt tgtgtgtcca tggtggttta tctcctgata 840
tcaaaacact ggatcaaatt cgaaccatcg aacggaatca ggaaattcct cataaaggag 900
cattttgtga tctggtttgg tcagatcctg aagatgtgga tacctgggct atcagtcccc 960
gaggagcagg ttggcttttt ggagcaaagg tcacaaatga gtttgttcat atcaacaact 1020
taaaactcat ctgcagagca catcaactag tgcacgaagg ctataaattt atgtttgatg 1080
agaagctggt gacagtatgg tctgctccta attactgcta tcgttgtgga aatattgctt 1140
cgatcatggt cttcaaagat gtaaatacaa gagaaccaaa gttattccgg gcagttccag 1200
attcagaacg tgttattcct cccagaacga caacgccata tttcctttga ggccttcgcc 1260
catcctgctg acccattttt ctgccctctt cttaccccaa ttttcttgta ttaccctcta 1320
caatatactt tttattgagc actttgctgc tgaaatgctg cctcttgcct tttttttttt 1380
aaattttaaa ttatctaaat ttattgtttg ttgtggtgtc tatagcaaag tttttctatc 1440
aattttcccc catcccatcc ccaccctgga ctcatttgag aagacttgag aaatgtctta 1500
atactcacac tgctgcatgt agctcttgct tatttactgg tctgggaaac aggatgtgtt 1560
tccttttttt aaaagccaat tgacagatta cacctaaata ctcctccttt tgtatcattc 1620
agccttttgt tttagtttgg taagttttaa gaaatttcag cagcaaagtt gttattcagt 1680
gggcacgatg gactccaaat gcctcaagtt atgtatacct gtcccagatg taaacttcat 1740
tgtcctttgt tggatgatat tttaaatgga tataaaataa attggtctaa agggctgccc 1800
tccttgttgt gtttttaaat tttagttaaa aactgctaca gcttatgact ttgtacttta 1860
agataattgt attgatcttt tttcagattc cttgtatttt ttaataaagt aatcttaaat 1920
aaaactcaga taggttaagt gttagaaatt ttaaacagct tacattgtta gcgtaaagtt 1980
atcttttctt ttttcctaat cagagttctt gaccctttgg ttattgagtt taaaacttca 2040
attgaaattc aatagtattt atttttgaaa aaaatcacta aactgtgcct aaagaacata 2100
actgccatat taatgttttg gtttatatcc tctatagtaa tagaaaaaca tttaatactt 2160
gtaatgctga tgtgttaatt tgataccagt tgagtagaat gtgatcaatc cagtttacaa 2220
tctatcatga gtattattaa ctaaaatcta tgtgcttttc aataggaatc attcttctct 2280
tgctgtaaca cttgacctta acttttagaa agtgttcatt tttaaactgc aactggaaag 2340
gttgaaaagt taggactctt gtatttgtga actgtaatct gaagcagatt atttaaagtg 2400
tagaaaaaga aacaagttct tcttttttgc aaaggtctgt gataccatat ttcagctttg 2460
tgtaagtaat ttgaatatcc aaagggttgt gatgatcagt tctgaatatg caactgtcca 2520
cttaataagg acaagtattc cagtatctct tatgactgta gtcataaatg atgttggaat 2580
gtacattttg tgaaatagtt ggtatccctt tactatgatt aatttttgtt attccaggaa 2640
atacttgtga agccagccaa ttaataaagc actttagcat ctgttcaggt agttttgaaa 2700
acccactttt ccccttcagg ataagaactt ccaggttacc taaaaatgca ataaaaatct 2760
ttatagtcta agcttcttgg catataattt ttcatcaggg ttttctttta ttaattgagc 2820
acaatactgt tttgatttaa tttttttccc tcaaaccacc cagctcctta catagttttt 2880
aattatatag ctatatgaaa gaggtggcta agacatttgc tgcacacact tgaactaatg 2940
ttgggtcagt agcttcgtgt tactgccctg atcccagtgt aattcaggtg gaaaggtatt 3000
tttatctcac agggatatgt agctatgtat tttactaatt gtgaaacact ggaaattaat 3060
gatgcagaca acttggtgtg gtctttgaac agctctctgc agtatttttt tttttcctgt 3120
taccataaat tgtatttaag tgcttgcttt ccacttaagt tgtactaata gaaccggtaa 3180
ctcccagccc tccctgtttg acactcctta gcttagattg atgtagttgt ttttgttatc 3240
cctataatgt gacttttatt tttaagtcat tgtgtactgc atttgtttgt cactagttgg 3300
gcacgtgcca ataatattct ctccattcct tatctgccat ctctgttttg cctgatttct 3360
cttcaactga ataatggctt tttgcatgga aaaaatagtt tttactatta gacgtgtaaa 3420
gggaagagag agctaatgta ttggactttg tgagcctaca aggaatattt tggatccctc 3480
caataaataa gggctatgta ctatatgtac tatatagagt tatcatgtgg tggaagatac 3540
ttgcaagtca tagatttatg ggcaggagga tttgttactc cctatatcta ggctgaatgt 3600
aaaatccctt atgttgtatc aatgggggta aaaactattt ttatttgcct atgatatact 3660
tggtttctaa taaagtgccc taggctctag tgagaactgt ctactttgaa ttgccattta 3720
ctccctttcc tcctttggcc gatatgctct tggctagctt tttataagtt aatgtgtttc 3780
cccaaaaagt ttcactactt tatattcatt tgagtgtgat cctaaaacac ctggatcaac 3840
agtacatctc atatgcaatc tgcatcagct cctattctgt ctggatgtct agaactgttg 3900
gaagattttg acgtcttaag ccctaggttt tgctttggga aataaggttt gaaatattgt 3960
tcattgcatt aagatttgtg tgtgtgtgct ttgtaagccc agaaccaggt tttggaaaat 4020
gcctgtactg tgaaagcaaa ttaggactct ttctgagcct ctttcattgt cagaaataga 4080
atcactttcc atcagcttcc aggaaattgt gtatctggag tcgaagagat ttgacatcaa 4140
gaatccagat ttttaaatgt aattgttttt taaatgctaa tgtttgtaaa gcaccttcag 4200
ttcttcggat gaaaggtgct atattccctc agtgtaaatt aataaaaaga ttacaggaag 4260
tttgtcaaaa aattcaatgc atagtctgta gtatgtcctg acaagaagtt agcattttat 4320
ataagaaatt aaaaaatgct tattcctcca aaaaaaaaaa aaaaaaa               4367

<210> 4
<211> 80
<212> RNA
<213> Artificial Sequence

<220> 
<223> guide RNA scaffold sequence

<400> 4
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu 60
ggcaccgagu cggugcuuuu                                             80

<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA1

<400> 5
gcacatcaac tagtgcacga                                             20

<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA2

<400> 6
cctaattact gctatcgttg                                             20

<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA3

<400> 7
ggaactgccc ggaataactt                                             20

<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA4

<400> 8
tcctcgggga ctgatagccc                                             20

<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA5

<400> 9
aatttcctga ttccgttcga                                             20

<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> sgRNA6

<400> 10
cgcaaaagtg taatacgatc                                             20

<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> neg sgRNA

<400> 11
gcactaccag agctaactca                                             20

<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> BCL11A sgRNA

<400> 12
ctaacagttg cttttatcac                                             20
