               SEQUENCE LISTING

<110> Technische Universiteit Delft

<120> Recombinant fungal cell

<130> P1600135NL00

<160> 10

<170> BiSSAP 1.3.6

<210> 1
<211> 1968
<212> DNA
<213> Artificial Sequence


<220> 
<223> TtTHC1 - codon-optimized sequence based on THC1 gene of
      Tetrahymena thermophila

<400> 1
atgaagaaga tcttgatcgg tttgatcatc ggtttgttct tgttctcttc tgttaacgct      60

tctgttaact tgactgaagt tcaaaacgct atctctatcc aacaaggtat caactgggct     120

gaagttcaca acaacacttg gtactaccca ccatacttgg gtgaaatgtt catctctgaa     180

tactacttcg aattgttggt tttgaactgg actcacaagt ctgctttcaa cgctacttac     240

ttcactgaaa gattgttgca aactcaattc gaagacggtt cttgggaaca agttagagaa     300

caaaacttgg aaactggtca attggacgct actgttttca actactggta cttgaagtct     360

atcaacaaca acccaaagat cgaagctgct ttgcaaaagg ctagaaagtg gatcgttgct     420

caaggtggta tcgaagctac tcaaactatg actaagttca agttggctgc tttcggtcaa     480

tactcttggg aagacttgtg gtacgttcca ttgttcatct tcaagcaaaa cggtatcttc     540

aagtacactt acgttaagga catcgttgct caatgggttt acccacactt gactgctttg     600

gcttacttga gataccaaag aactgttttc aacgttccag ttgctgactt gagagaattg     660

tggatcaact acccaaagaa cggtatcaag atctctccaa gagaatactc tactttgaac     720

ccagactctg acttgttgat cttgatggac gaaatcttca agttgaagca accattgggt     780

tctttcggtg cttacactat ctctactttg ttgactttga tgtctttcaa ggacttccaa     840

tctaagcacc cacacttgta ccaaaacgaa atccaaaagg cttacgaaga cggttactac     900

ttcgttgaat tcaactactt caacttcaga gaagcttacc acggttcttt ggacgacggt     960

agatggtggg acactatctt gatctcttgg gctatgttgg aatctggtca agacaaggaa    1020

agaatcttcc caatcgttca aaacatggtt aaggaaggtt tgcaaccaaa gaagggtatc    1080

ggttacggtt acgacttcga atacgctcca gacactgacg acactggttt gttgttggtt    1140

gttatgtctt actacaagga agctttccaa aagcaaatcc cagaaactat cgaatggttg    1200

ttctctatgc aaaacgacga cggtggttac ccagctttcg acaagggtaa gaacgaagac    1260

aacttgttgt tcaagttcgc tttcaacatg gctggtatcg ctaactctgc tgaaatcttc    1320

gacccatctt gtccagacat cactggtcac atcatggaag gtttgggtga attcggttac    1380

caagctaacc acccacaaat ccaaaacatg atcaagtacc aaagaaagac tcaaaacaag    1440

tggggttctt ggcaagctag atggggtgtt aactacatca tggctgttgg tgctgttgtt    1500

ccaggtttgg ctagagttaa ctacgacttg aacgaacaat gggttcaaaa ctctatcaac    1560

tacttgttga acaagcaaaa caaggacggt ggtttcggtg aatgtgtttt gtcttacaac    1620

gacccagaaa agtggaacgg tatcggtaag tctactgtta ctcaaacttc ttggggtttg    1680

ttggctttgt tggaagttta caaccaaaac gaacaaatca agcacgctgc tgacagagct    1740

gctcaatact tgttggacca attcaagaga gacgacaaca ctttctacga ccactctact    1800

atcggtactg gtcacagagg tttgttgtac ttgcaatacc catcttacgc tcaatctttc    1860

ccattggttg ctttgaacag ataccaaaag atctctcaag gtcaatacca cttctctaag    1920

aacttgtaca acggtaacgg tgaaccagtt caaaagcaaa acatctaa                 1968


<210> 2
<211> 1971
<212> DNA
<213> Artificial Sequence


<220> 
<223> SjSHC - codon-optimized sequence based on SHC gene of
      Schizosaccharomyces japonicus CBS5679 yFS275

<400> 2
atgaaaactg atggtaacac tactttggac accaccattt ctatggaaga attggaaaga      60

accgttaagt ctgcttatga agctttggct aaggatcaac aagatgatgg tcattggatc     120

tacgaattgg aagctgatgt tactattcca gctcagttca ttttgttgga acacaccttg     180

gataagatcg acgaagagtt ggaacaaaag attgccaact acttgagaag atgccaatct     240

agagaacatt ggggttggcc agtttattat ggtggtgaat tcaatatctc cgcttccgtt     300

caagcttact ttgctttgaa aatgaccggt gaagatatta acgctccaca tatggttaga     360

gctagagaag ctattttggc tcatggtggt ccagaatacg ctaatgtttt caccagaatc     420

cagttgtctt tgttcggtga agcttcttgg ttggctactc cttttatgcc agttgaaatt     480

atgttgttgc caaggtggat gtacttctcc atttggaata tgtcttactg gtctagaact     540

accgttgctc cattattgat agttgctgat ttgaagccaa aggctatcaa cccaagaaac     600

gttcatattc cagagttgtt tccaactcca ccagataagg ttaagacttg gattcatggt     660

ccattcagat caaaatgggg tcacgttttc aagttcattg ataccgctat tagaccattc     720

accagattcg ttccatcttt cttgcataag aaagcttaca aggctgcctt ggatttcatt     780

gaacctagat tgaatggtgt tgatggtttg ggtgctatct atccaccaat gtcttattct     840

gctgttatgt acagagcttt gggtatccca gatgatgatc caagagctgc tactaattgg     900

gaagctttga aaggtttgtt ggtcatcaaa gaaagagaag cttactgtca agcttgcgtt     960

tctccagttt gggatactgc tttgtctggt catgctttga tggaagcttc atttggtcca    1020

gatggtatta acgcagatag aaccgaaaag ttgattgata gagctgctca ttggttgaga    1080

gcacatcaag ttttgaatgt tgttggtgat tgggccatta acaacccaaa cttgcaacca    1140

ggtggttggg cttttcaata tggtaatgat tactacccag atgttgatga tactgctgtt    1200

gctgctatgt tgctgcatag acaaaatttg ccagaaaacg aagaagcctt ggatagagcc    1260

agaaaatgga ttattggtat gcaatcttct aatggtggat ggggtgcttt cgatattgat    1320

aacgacaagc aaatcttgaa cgatatccca tttgctgatc atggtgcttt gttggatcca    1380

ccaactgctg atgtttctgc tagatgtatt tctttgttgg ccgaattggg tcatccagaa    1440

gatagaccag ttattgaaag aggcattaag tacctgcgta aagaacaaga ggaagatggt    1500

tcttggtttg gtagatgggg tactaattac atctatggtg cttggtctgt tttgtgtgct    1560

tttaatgctt ctggtgttcc acacgatgat ccatctgttt tgaagtgtgt taacttcttg    1620

aagtccgtcc aacgtgaaga tggcggttgg ggtgaatctt gtgaaactta tgaaggttct    1680

gcacatggtg tttacactga atctttgcca tctcaaactg cttgggcagt tttgggtttg    1740

atggcttctg gtagaagaac tgatccagct gttaagagag gtatcgtttg gttgattcaa    1800

caccaacaag ataacggtga atgggctgaa gaacctttta acgctgttgg ttttccaagg    1860

atgttctact tgcattactt gggttacaag cagttcttcc cattattggc tttagccaga    1920

tacagacaca tggaaaaatc tggcactaac aatgtttctt tcgccttctg a             1971


<210> 3
<211> 2687
<212> DNA
<213> Artificial Sequence


<220> 
<223> TEF1-promotor - TtTHC1 ORF - CYC1-terminator

<400> 3
catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc      60

atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct     120

ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt     180

ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa     240

tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac     300

ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact     360

tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac gcgataccct     420

gcgatcttca tgaagaagat cttgatcggt ttgatcatcg gtttgttctt gttctcttct     480

gttaacgctt ctgttaactt gactgaagtt caaaacgcta tctctatcca acaaggtatc     540

aactgggctg aagttcacaa caacacttgg tactacccac catacttggg tgaaatgttc     600

atctctgaat actacttcga attgttggtt ttgaactgga ctcacaagtc tgctttcaac     660

gctacttact tcactgaaag attgttgcaa actcaattcg aagacggttc ttgggaacaa     720

gttagagaac aaaacttgga aactggtcaa ttggacgcta ctgttttcaa ctactggtac     780

ttgaagtcta tcaacaacaa cccaaagatc gaagctgctt tgcaaaaggc tagaaagtgg     840

atcgttgctc aaggtggtat cgaagctact caaactatga ctaagttcaa gttggctgct     900

ttcggtcaat actcttggga agacttgtgg tacgttccat tgttcatctt caagcaaaac     960

ggtatcttca agtacactta cgttaaggac atcgttgctc aatgggttta cccacacttg    1020

actgctttgg cttacttgag ataccaaaga actgttttca acgttccagt tgctgacttg    1080

agagaattgt ggatcaacta cccaaagaac ggtatcaaga tctctccaag agaatactct    1140

actttgaacc cagactctga cttgttgatc ttgatggacg aaatcttcaa gttgaagcaa    1200

ccattgggtt ctttcggtgc ttacactatc tctactttgt tgactttgat gtctttcaag    1260

gacttccaat ctaagcaccc acacttgtac caaaacgaaa tccaaaaggc ttacgaagac    1320

ggttactact tcgttgaatt caactacttc aacttcagag aagcttacca cggttctttg    1380

gacgacggta gatggtggga cactatcttg atctcttggg ctatgttgga atctggtcaa    1440

gacaaggaaa gaatcttccc aatcgttcaa aacatggtta aggaaggttt gcaaccaaag    1500

aagggtatcg gttacggtta cgacttcgaa tacgctccag acactgacga cactggtttg    1560

ttgttggttg ttatgtctta ctacaaggaa gctttccaaa agcaaatccc agaaactatc    1620

gaatggttgt tctctatgca aaacgacgac ggtggttacc cagctttcga caagggtaag    1680

aacgaagaca acttgttgtt caagttcgct ttcaacatgg ctggtatcgc taactctgct    1740

gaaatcttcg acccatcttg tccagacatc actggtcaca tcatggaagg tttgggtgaa    1800

ttcggttacc aagctaacca cccacaaatc caaaacatga tcaagtacca aagaaagact    1860

caaaacaagt ggggttcttg gcaagctaga tggggtgtta actacatcat ggctgttggt    1920

gctgttgttc caggtttggc tagagttaac tacgacttga acgaacaatg ggttcaaaac    1980

tctatcaact acttgttgaa caagcaaaac aaggacggtg gtttcggtga atgtgttttg    2040

tcttacaacg acccagaaaa gtggaacggt atcggtaagt ctactgttac tcaaacttct    2100

tggggtttgt tggctttgtt ggaagtttac aaccaaaacg aacaaatcaa gcacgctgct    2160

gacagagctg ctcaatactt gttggaccaa ttcaagagag acgacaacac tttctacgac    2220

cactctacta tcggtactgg tcacagaggt ttgttgtact tgcaataccc atcttacgct    2280

caatctttcc cattggttgc tttgaacaga taccaaaaga tctctcaagg tcaataccac    2340

ttctctaaga acttgtacaa cggtaacggt gaaccagttc aaaagcaaaa catctaagct    2400

tcgctaatct gcgcgacagg ccccttttcc tttgtcgata tcatgtaatt agttatgtca    2460

cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa    2520

cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt    2580

atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg    2640

aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttg                  2687


<210> 4
<211> 2639
<212> DNA
<213> Artificial Sequence


<220> 
<223> TEF1-promotor - SjSHC ORF - CYC1-terminator

<400> 4
gctggagctc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg      60

actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc     120

tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga     180

ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt     240

tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag     300

ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact     360

ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt tttatgaaaa     420

ctgatggtaa cactactttg gacaccacca tttctatgga agaattggaa agaaccgtta     480

agtctgctta tgaagctttg gctaaggatc aacaagatga tggtcattgg atctacgaat     540

tggaagctga tgttactatt ccagctcagt tcattttgtt ggaacacacc ttggataaga     600

tcgacgaaga gttggaacaa aagattgcca actacttgag aagatgccaa tctagagaac     660

attggggttg gccagtttat tatggtggtg aattcaatat ctccgcttcc gttcaagctt     720

actttgcttt gaaaatgacc ggtgaagata ttaacgctcc acatatggtt agagctagag     780

aagctatttt ggctcatggt ggtccagaat acgctaatgt tttcaccaga atccagttgt     840

ctttgttcgg tgaagcttct tggttggcta ctccttttat gccagttgaa attatgttgt     900

tgccaaggtg gatgtacttc tccatttgga atatgtctta ctggtctaga actaccgttg     960

ctccattatt gatagttgct gatttgaagc caaaggctat caacccaaga aacgttcata    1020

ttccagagtt gtttccaact ccaccagata aggttaagac ttggattcat ggtccattca    1080

gatcaaaatg gggtcacgtt ttcaagttca ttgataccgc tattagacca ttcaccagat    1140

tcgttccatc tttcttgcat aagaaagctt acaaggctgc cttggatttc attgaaccta    1200

gattgaatgg tgttgatggt ttgggtgcta tctatccacc aatgtcttat tctgctgtta    1260

tgtacagagc tttgggtatc ccagatgatg atccaagagc tgctactaat tgggaagctt    1320

tgaaaggttt gttggtcatc aaagaaagag aagcttactg tcaagcttgc gtttctccag    1380

tttgggatac tgctttgtct ggtcatgctt tgatggaagc ttcatttggt ccagatggta    1440

ttaacgcaga tagaaccgaa aagttgattg atagagctgc tcattggttg agagcacatc    1500

aagttttgaa tgttgttggt gattgggcca ttaacaaccc aaacttgcaa ccaggtggtt    1560

gggcttttca atatggtaat gattactacc cagatgttga tgatactgct gttgctgcta    1620

tgttgctgca tagacaaaat ttgccagaaa acgaagaagc cttggataga gccagaaaat    1680

ggattattgg tatgcaatct tctaatggtg gatggggtgc tttcgatatt gataacgaca    1740

agcaaatctt gaacgatatc ccatttgctg atcatggtgc tttgttggat ccaccaactg    1800

ctgatgtttc tgctagatgt atttctttgt tggccgaatt gggtcatcca gaagatagac    1860

cagttattga aagaggcatt aagtacctgc gtaaagaaca agaggaagat ggttcttggt    1920

ttggtagatg gggtactaat tacatctatg gtgcttggtc tgttttgtgt gcttttaatg    1980

cttctggtgt tccacacgat gatccatctg ttttgaagtg tgttaacttc ttgaagtccg    2040

tccaacgtga agatggcggt tggggtgaat cttgtgaaac ttatgaaggt tctgcacatg    2100

gtgtttacac tgaatctttg ccatctcaaa ctgcttgggc agttttgggt ttgatggctt    2160

ctggtagaag aactgatcca gctgttaaga gaggtatcgt ttggttgatt caacaccaac    2220

aagataacgg tgaatgggct gaagaacctt ttaacgctgt tggttttcca aggatgttct    2280

acttgcatta cttgggttac aagcagttct tcccattatt ggctttagcc agatacagac    2340

acatggaaaa atctggcact aacaatgttt ctttcgcctt ctgatcatgt aattagttat    2400

gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag    2460

acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt    2520

atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat    2580

actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggt     2639


<210> 5
<211> 2881
<212> DNA
<213> Artificial Sequence


<220> 
<223> AgTEF1p-KanMX-AgTEF1t

<400> 5
ggataagctg ttcacaaaat ctcttctcga gtatgggttc gatgtggcta ttccaagcaa      60

cgcaagaagg tttttaccaa acgatgaaga gttaagagat tcttataaat acggctccaa     120

cgttggaggt tcgcattacg cctatctaat ctcctcattc gacattcccg aaggtgacaa     180

tgacaagttt tggagtcttg tcgaatacta ctatgaccgc tttttagaat cgtacgacaa     240

cggtgaccac ttgattggtc tgggggtcct acaacttgat tttatcgttg aaaacaagaa     300

tatagacagc cttcttgcca actcttattt gcaccagcaa agaggcggtg caatcatcag     360

taatacagga cttgtctcgc aagatacgac caagccgtac tacgttcggg atttaatctt     420

ctcgcagtct gcaggcgcct tgagatttgc gttcggccta aacgtttgct ccacaaacgt     480

gaatggtatg aacatggaca tgagcgtggt tcagggcact ctacgggatc gtggcgaatg     540

ggaatcgttc tgcaagctct tctaccaaac catcggcgaa tttgcgtcgc tttaatgcga     600

tactgccgta gcgggccttc gtatagctcg gccgagctcg tacaaaaggc aagcagtgta     660

tcggacagag ctgatataac acaatacgct cgtagtcgat gcatgccgtg gctgctctcg     720

gtcgggtata agtcttagac aatagtctta cctcgcatgt cgacatggag gcccagaata     780

ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca     840

tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg     900

cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca     960

cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt    1020

tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc    1080

acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg    1140

attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg    1200

gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct    1260

gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg    1320

gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc    1380

atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc    1440

tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat    1500

tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc    1560

acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc    1620

tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt    1680

cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg    1740

tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa    1800

ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga    1860

taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt    1920

actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt    1980

gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc    2040

atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga    2100

atgctggtcg ctatactgct gtcgattcga tatgcttcaa aatatgcatt gagattcata    2160

ttcggttatc aattaacgat attacttcct taagtgatat taaatcaagc ttgccggatt    2220

ctgcagtcag aattatacat aatagtattt gtgctctcgt aaatcctttc agaattacac    2280

catagtagtc tttgtgaatg ttctacttat tagaatagat tattgctagt ttatctcact    2340

tttcattccc catatccctt tttttgctca tcccccgggg tattttatca agataaaatt    2400

ttatatacat gcagtgcgcg ataactccta gagaggcagt aatagcaaaa caaagacagt    2460

ataagcatta tttgggtatg gaaagaccat tgtgggtacg ttggttgaaa gtctatgcaa    2520

ttggtggcgc tattattgga agcgggtttc tgcttttcaa gtacacaacg ccaacagatc    2580

aacagttaat cagccagctt tccccagaat tacgtttaca atatgagagg gagaaaaaac    2640

tacgtcagtc ggagcagcag gctttgatga aaattgttaa ggaaacttct caaagtgacg    2700

atccaatttg gaagacgggc ccccttcaat caccttggga aaggaacggt gataacgttc    2760

aaagcagaga tcactttgct aaggtgaggg cggaggaggt gcaaaaagaa gagttggcta    2820

ggatcagaaa tgaactgtct cagctaagat ctgaaacgga ggagaaaaca aaggaaatag    2880

t                                                                    2881


<210> 6
<211> 3205
<212> DNA
<213> Artificial Sequence


<220> 
<223> KmPDC1-promotor - TtTHC1 ORF - ScADH1-terminator

<400> 6
tccagcgaat atacagcgtg aataatggaa tggccttgta ttcgtttttt ccgagagaaa       60

aaaacgggct tcggtgaaaa tcgggtgaat atgcaactag cgggacgaat gctctggaaa      120

tgcatatcct atgcaactag cgggatgaac aaatctcacc ccagaattcg caggaaaaaa      180

caggaaaaaa aaaaagaagg ccaccacggc cacaaagacc acaaagacca caaaaaaaaa      240

caaaaaacaa ccgtcccagc ttccagtgtt tggaatactg gaacacagga agccgcataa      300

gagtgggcgt tgcacaggaa gccaggccca gaagccccag agttactttt ttttttttgt      360

tttttccttc tgttcgctgt gcccgcatca gatgatgcgc ctttatttac gatgccaatg      420

cgaatagcac cagtgagagc accagtaaaa gcatacgcat acacatacac acatagagca      480

agcaagcagg ctagcaacca ggaaaggctg ccagtgactg ctactgggtg tctaagaacc      540

gtagggcgga ttattgttgc ggtggttggt tgcgggtggt tatgcgatgg tacggtgcag      600

aatcgtacgg tgttggttat ggaattagta tgggtatgtg atatgtggta atatgtgata      660

ttgggttatt gtgatttgga atactgaata tcgaatatgg gatatggaat atggccatgg      720

catggtatgg tatgggatgg gagtattcta ttttatttta ttttattctg gttcctgcgt      780

ttagggtagg gtaggaagaa ggtgagtgct tttgtatata agtggagtgt ctggatcagt      840

tttgtggatt gtgaatgtta gtttcccctt taatgtatat ttgtattatt tgcttttgag      900

tactcaataa ccaagcacaa ctactagttt taaaggatcc atcctcttaa acagtacaaa      960

tcgcaaagaa aagctccaca cccaaaccaa ataattgcta tgaagaagat cttgatcggt     1020

ttgatcatcg gtttgttctt gttctcttct gttaacgctt ctgttaactt gactgaagtt     1080

caaaacgcta tctctatcca acaaggtatc aactgggctg aagttcacaa caacacttgg     1140

tactacccac catacttggg tgaaatgttc atctctgaat actacttcga attgttggtt     1200

ttgaactgga ctcacaagtc tgctttcaac gctacttact tcactgaaag attgttgcaa     1260

actcaattcg aagacggttc ttgggaacaa gttagagaac aaaacttgga aactggtcaa     1320

ttggacgcta ctgttttcaa ctactggtac ttgaagtcta tcaacaacaa cccaaagatc     1380

gaagctgctt tgcaaaaggc tagaaagtgg atcgttgctc aaggtggtat cgaagctact     1440

caaactatga ctaagttcaa gttggctgct ttcggtcaat actcttggga agacttgtgg     1500

tacgttccat tgttcatctt caagcaaaac ggtatcttca agtacactta cgttaaggac     1560

atcgttgctc aatgggttta cccacacttg actgctttgg cttacttgag ataccaaaga     1620

actgttttca acgttccagt tgctgacttg agagaattgt ggatcaacta cccaaagaac     1680

ggtatcaaga tctctccaag agaatactct actttgaacc cagactctga cttgttgatc     1740

ttgatggacg aaatcttcaa gttgaagcaa ccattgggtt ctttcggtgc ttacactatc     1800

tctactttgt tgactttgat gtctttcaag gacttccaat ctaagcaccc acacttgtac     1860

caaaacgaaa tccaaaaggc ttacgaagac ggttactact tcgttgaatt caactacttc     1920

aacttcagag aagcttacca cggttctttg gacgacggta gatggtggga cactatcttg     1980

atctcttggg ctatgttgga atctggtcaa gacaaggaaa gaatcttccc aatcgttcaa     2040

aacatggtta aggaaggttt gcaaccaaag aagggtatcg gttacggtta cgacttcgaa     2100

tacgctccag acactgacga cactggtttg ttgttggttg ttatgtctta ctacaaggaa     2160

gctttccaaa agcaaatccc agaaactatc gaatggttgt tctctatgca aaacgacgac     2220

ggtggttacc cagctttcga caagggtaag aacgaagaca acttgttgtt caagttcgct     2280

ttcaacatgg ctggtatcgc taactctgct gaaatcttcg acccatcttg tccagacatc     2340

actggtcaca tcatggaagg tttgggtgaa ttcggttacc aagctaacca cccacaaatc     2400

caaaacatga tcaagtacca aagaaagact caaaacaagt ggggttcttg gcaagctaga     2460

tggggtgtta actacatcat ggctgttggt gctgttgttc caggtttggc tagagttaac     2520

tacgacttga acgaacaatg ggttcaaaac tctatcaact acttgttgaa caagcaaaac     2580

aaggacggtg gtttcggtga atgtgttttg tcttacaacg acccagaaaa gtggaacggt     2640

atcggtaagt ctactgttac tcaaacttct tggggtttgt tggctttgtt ggaagtttac     2700

aaccaaaacg aacaaatcaa gcacgctgct gacagagctg ctcaatactt gttggaccaa     2760

ttcaagagag acgacaacac tttctacgac cactctacta tcggtactgg tcacagaggt     2820

ttgttgtact tgcaataccc atcttacgct caatctttcc cattggttgc tttgaacaga     2880

taccaaaaga tctctcaagg tcaataccac ttctctaaga acttgtacaa cggtaacggt     2940

gaaccagttc aaaagcaaaa catctaaatc ctaactcgag gcgaatttct tatgatttat     3000

gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc     3060

ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct     3120

ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa     3180

atgcctgcaa atcgctcccc atttc                                           3205


<210> 7
<211> 1959
<212> DNA
<213> Artificial Sequence


<220> 
<223> SjSHC_2 - codon-optimized sequence based on SHC gene of
      Schizosaccharomyces japonicus CBS5679

<400> 7
atgaaaactg atggtaacac tactctggac acctccattt ctatggaaga tttggaaaga      60

accgttaagt ctgcttatga agctttggct aaggaccaac aagaagatgg tcattggatc     120

tatgaattgg aagccgatgt tactattcca gctcaattca tcttgttgga acacaccttg     180

gataagatcg acgaagaatt ggaacaaaag atcgccaact acttgagaag atgccaatct     240

agagaacatt ggggttggcc agtttattat ggtggtgaat tcaatatctc cgcttccgtt     300

caagcttact ttgctttgaa aatgaccggt gaagatatta acgctccaca tatggttaga     360

gctagagatg ctattttggc tcatggtggt ccagaatacg ctaatgtttt caccagaatc     420

cagttgtctt tgtttggtga agctccatgg ttggctactc cattcatgcc agttgaaatt     480

atgttgttgc caaggttcat gtacttctcc atttggaata tgtcctactg gtctagaact     540

actgttgctc cattattgat cgttgctgat ttgaagccaa aggctattaa cccaagaaac     600

gttcatattc cagagttgtt tccaactcca ccagataagg ttaagacttg gattcatggt     660

ccattcagat caaaatgggg tcacgttttc agattcattg ataccgctat tagaccattc     720

accagattcg ttccatcctt cattcataag agagcttaca aagctgcctt ggatttcatt     780

gaacctagat tgaatggtgt tgatggtttg ggtgctatct atccaccaat gtcttattct     840

gctgttatgt acagagcttt gggtatccca gatgatgatc caagagctgc tactaattgg     900

gaagctttga aaggtttgtt ggtcatcaaa gaaagagaag cttactgtca agcttgcgtt     960

tctccagttt gggatactgc tttatcttct catgctttga tggaagcttc atttggtcca    1020

tatggtatta acgccgatag aaccgaaaag ttgattgata gagctgcttc ttggttgaga    1080

gcacatcaag ttttgaatgt tgttggtgat tgggccatta acaacccaaa cttgcaacca    1140

ggtggttggg cttttcaata tggtaatgat tactacccag atgttgatga tactgctgtt    1200

gctgctatgt tgctgcatag acaaaatttg ccagaaaatg ctgaagcctt ggatagagca    1260

agaaagtgga ttattggtat gcaatcttct tctggtggat ggggtgcttt tgatattgat    1320

aacgataagc agatcctgaa cgatattcca tttgctgatc atggtgcttt gttggatcca    1380

ccaactgctg atgtttctgc tagatgtatt tctttgttgg ccgaattggg tcatccagaa    1440

gatagatctg ttatcgaaag aggcattaag ttcttgcgta aagaacaaga ggaagatggt    1500

tcttggtttg gtagatgggg tactaattac atctatggtg cttggtctgt tttgtgtgct    1560

tttaacgctt gtggtattcc acacgatgat ccatctgtta ttaaggccgt taacttcttg    1620

aagtccatcc aacgtgaaga tggcggttgg ggtgaatctt gtgctactta tgaaggtgct    1680

ccacatggta ttcatatcga atctttgcca tctcaaactg cttgggcagt tttgggtttg    1740

atggcttctg gtagaagaac tgatccagca gttaagagag gtatagtttg gttgatgcaa    1800

caccaacagg ataacggtga atgggctgaa gaacctttta atgctgttgg ttttccaagg    1860

atgttctact tgcattactt gggttacaag cagttcttcc cattgatggc attggctaga    1920

tatcatcaca tggaaatgtc tggtacaaac aacgtctga                           1959


<210> 8
<211> 1845
<212> DNA
<213> Artificial Sequence


<220> 
<223> MaTHS - codon-optimized sequence based on THS gene of
      Methylomicrobium alkaliphilum

<400> 8
atgacaaaaa atgccaactt gtacgacatc tgcgataacc caattttgaa aaccgaatct      60

ccatctacac ctggtcaaaa cttgagactg atctacaaaa agaccattgg tcatccagcc     120

ttgaagtact ttattttgaa gggttgtaga cacccctcca tcaactttga acaaattcca     180

atctaccagt ccatgatgca agaagcaatg caagcttcta ctgaacaatg gcaagataag     240

caatggatta cctctacttt tcaaccattg gctcatttgt tggacaccat cgaaaacaga     300

gattggcaaa tcagagatag ctctaatcca tctcaagcta agacctctca aatcgattgc     360

catgttatta ttgatgcctg cttgcaagac atcttcagag tttggaatca agataagaac     420

gatccatggt ttccagttgg tgctcaagtt caattgtctg gtgatgatca tatggacggt     480

aagaacttgt tggatgtatt gcaaggtgtt ggttcttacg agtacaagaa cattactttg     540

ttgttcgcct tgatcaggtg tttcttgatg ccaaatccaa acagattgaa gtggttcaga     600

aaaccataca gaggtatttg cgaacctatg tctgctagat tttcttggat ctggcataga     660

attgccttct ccgatgttaa cttcttcgaa cacttgctgg tgttcttgga atctaatgct     720

gctcatagac agtacaacga aagattgatt cccatcctgg aaaacttgtt gcactactct     780

ttgtctactt ccagagaatg gttgactacc ccaaacaaac atattagaca tccagctatt     840

acctgcttgc cagttgatgc taaaggtaag caattgtgca gattgtctga ttcctcttgg     900

caaaaaaaac gtgacttagg tttcggtgat tacgttccag atactgatac aacttttttg     960

gctttggcta tggccaagaa gtggattaac ttcgtccaaa aagaaaactt gcaggtcgac    1020

agatccttgt tagatgaatg tgaatctatg ttggctttcc catgggttga aattattgcc    1080

gaatatcaag ttggtggctc ctacgaatct aatttgccaa ctattaagtt ggccaggcca    1140

ttggattatg atggtgctgt tccaatttgg ttcgaaaagg cttttactga taccgatggt    1200

aacatccaca gagaagtttt gggtaacgaa atttgtccag gtcacaactt ggatatcttg    1260

gactccattt tgatcaacag acgtcaatgg catgctttga ctggtgaaaa tttgatcttc    1320

ctgcacaagt tgttggactt tcatttcaga gctttcgtgt ccgataactt cagacatgaa    1380

tctgctttca tctactacct gccagaaatc tacacttact acttgggtag attctaccaa    1440

acctacagaa ctttgtctgc tgaagaaaga cagttgttcg atccagaaag aaaggttgaa    1500

accatgagag aaatcgcatt gcaatactgc aaggatgatt tgattggtca gaccttgaac    1560

gtttttgatg ctgctttggc tgtttctact ttggtgcaat tggaatacga acctaagcaa    1620

gatggtgtta tttctgctgg tttgaaggtt atcaacgacc acttaggtga aggtggtaat    1680

ggtcatcctt ttaaagctta cgaatggaac agaatgagac acccaactag aattttggtc    1740

ggttctgaaa tttccacctc cttgtttgtt ttgagagctt gttctgaagc ccaatcctac    1800

ttggattcta aagcttgtgc tttcccagaa tcttacccat cttaa                    1845


<210> 9
<211> 2614
<212> DNA
<213> Artificial Sequence


<220> 
<223> TEF1-promotor - SjSHC_2 ORF - CYC1-terminator

<400> 9
catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc      60

atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct     120

ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt     180

ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa     240

tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac     300

ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact     360

tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttatgaaa actgatggta     420

acactactct ggacacctcc atttctatgg aagatttgga aagaaccgtt aagtctgctt     480

atgaagcttt ggctaaggac caacaagaag atggtcattg gatctatgaa ttggaagccg     540

atgttactat tccagctcaa ttcatcttgt tggaacacac cttggataag atcgacgaag     600

aattggaaca aaagatcgcc aactacttga gaagatgcca atctagagaa cattggggtt     660

ggccagttta ttatggtggt gaattcaata tctccgcttc cgttcaagct tactttgctt     720

tgaaaatgac cggtgaagat attaacgctc cacatatggt tagagctaga gatgctattt     780

tggctcatgg tggtccagaa tacgctaatg ttttcaccag aatccagttg tctttgtttg     840

gtgaagctcc atggttggct actccattca tgccagttga aattatgttg ttgccaaggt     900

tcatgtactt ctccatttgg aatatgtcct actggtctag aactactgtt gctccattat     960

tgatcgttgc tgatttgaag ccaaaggcta ttaacccaag aaacgttcat attccagagt    1020

tgtttccaac tccaccagat aaggttaaga cttggattca tggtccattc agatcaaaat    1080

ggggtcacgt tttcagattc attgataccg ctattagacc attcaccaga ttcgttccat    1140

ccttcattca taagagagct tacaaagctg ccttggattt cattgaacct agattgaatg    1200

gtgttgatgg tttgggtgct atctatccac caatgtctta ttctgctgtt atgtacagag    1260

ctttgggtat cccagatgat gatccaagag ctgctactaa ttgggaagct ttgaaaggtt    1320

tgttggtcat caaagaaaga gaagcttact gtcaagcttg cgtttctcca gtttgggata    1380

ctgctttatc ttctcatgct ttgatggaag cttcatttgg tccatatggt attaacgccg    1440

atagaaccga aaagttgatt gatagagctg cttcttggtt gagagcacat caagttttga    1500

atgttgttgg tgattgggcc attaacaacc caaacttgca accaggtggt tgggcttttc    1560

aatatggtaa tgattactac ccagatgttg atgatactgc tgttgctgct atgttgctgc    1620

atagacaaaa tttgccagaa aatgctgaag ccttggatag agcaagaaag tggattattg    1680

gtatgcaatc ttcttctggt ggatggggtg cttttgatat tgataacgat aagcagatcc    1740

tgaacgatat tccatttgct gatcatggtg ctttgttgga tccaccaact gctgatgttt    1800

ctgctagatg tatttctttg ttggccgaat tgggtcatcc agaagataga tctgttatcg    1860

aaagaggcat taagttcttg cgtaaagaac aagaggaaga tggttcttgg tttggtagat    1920

ggggtactaa ttacatctat ggtgcttggt ctgttttgtg tgcttttaac gcttgtggta    1980

ttccacacga tgatccatct gttattaagg ccgttaactt cttgaagtcc atccaacgtg    2040

aagatggcgg ttggggtgaa tcttgtgcta cttatgaagg tgctccacat ggtattcata    2100

tcgaatcttt gccatctcaa actgcttggg cagttttggg tttgatggct tctggtagaa    2160

gaactgatcc agcagttaag agaggtatag tttggttgat gcaacaccaa caggataacg    2220

gtgaatgggc tgaagaacct tttaatgctg ttggttttcc aaggatgttc tacttgcatt    2280

acttgggtta caagcagttc ttcccattga tggcattggc tagatatcat cacatggaaa    2340

tgtctggtac aaacaacgtc tgatcatgta attagttatg tcacgcttac attcacgccc    2400

tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc    2460

tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct    2520

tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa    2580

ggttttggga cgctcgaagg ctttaatttg cggc                                2614


<210> 10
<211> 2826
<212> DNA
<213> Artificial Sequence


<220> 
<223> TDH3-promotor - MaTHS ORF - ADH1-terminator

<400> 10
agtttatcat tatcaatact cgccatttca aagaatacgt aaataattaa tagtagtgat      60

tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc     120

ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt     180

tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa     240

aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc     300

tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat     360

ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat     420

ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga     480

aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa     540

agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact     600

tttatagtta gtcttttttt tagttttaaa acaccagaac ttagtttcga cggatatgac     660

aaaaaatgcc aacttgtacg acatctgcga taacccaatt ttgaaaaccg aatctccatc     720

tacacctggt caaaacttga gactgatcta caaaaagacc attggtcatc cagccttgaa     780

gtactttatt ttgaagggtt gtagacaccc ctccatcaac tttgaacaaa ttccaatcta     840

ccagtccatg atgcaagaag caatgcaagc ttctactgaa caatggcaag ataagcaatg     900

gattacctct acttttcaac cattggctca tttgttggac accatcgaaa acagagattg     960

gcaaatcaga gatagctcta atccatctca agctaagacc tctcaaatcg attgccatgt    1020

tattattgat gcctgcttgc aagacatctt cagagtttgg aatcaagata agaacgatcc    1080

atggtttcca gttggtgctc aagttcaatt gtctggtgat gatcatatgg acggtaagaa    1140

cttgttggat gtattgcaag gtgttggttc ttacgagtac aagaacatta ctttgttgtt    1200

cgccttgatc aggtgtttct tgatgccaaa tccaaacaga ttgaagtggt tcagaaaacc    1260

atacagaggt atttgcgaac ctatgtctgc tagattttct tggatctggc atagaattgc    1320

cttctccgat gttaacttct tcgaacactt gctggtgttc ttggaatcta atgctgctca    1380

tagacagtac aacgaaagat tgattcccat cctggaaaac ttgttgcact actctttgtc    1440

tacttccaga gaatggttga ctaccccaaa caaacatatt agacatccag ctattacctg    1500

cttgccagtt gatgctaaag gtaagcaatt gtgcagattg tctgattcct cttggcaaaa    1560

aaaacgtgac ttaggtttcg gtgattacgt tccagatact gatacaactt ttttggcttt    1620

ggctatggcc aagaagtgga ttaacttcgt ccaaaaagaa aacttgcagg tcgacagatc    1680

cttgttagat gaatgtgaat ctatgttggc tttcccatgg gttgaaatta ttgccgaata    1740

tcaagttggt ggctcctacg aatctaattt gccaactatt aagttggcca ggccattgga    1800

ttatgatggt gctgttccaa tttggttcga aaaggctttt actgataccg atggtaacat    1860

ccacagagaa gttttgggta acgaaatttg tccaggtcac aacttggata tcttggactc    1920

cattttgatc aacagacgtc aatggcatgc tttgactggt gaaaatttga tcttcctgca    1980

caagttgttg gactttcatt tcagagcttt cgtgtccgat aacttcagac atgaatctgc    2040

tttcatctac tacctgccag aaatctacac ttactacttg ggtagattct accaaaccta    2100

cagaactttg tctgctgaag aaagacagtt gttcgatcca gaaagaaagg ttgaaaccat    2160

gagagaaatc gcattgcaat actgcaagga tgatttgatt ggtcagacct tgaacgtttt    2220

tgatgctgct ttggctgttt ctactttggt gcaattggaa tacgaaccta agcaagatgg    2280

tgttatttct gctggtttga aggttatcaa cgaccactta ggtgaaggtg gtaatggtca    2340

tccttttaaa gcttacgaat ggaacagaat gagacaccca actagaattt tggtcggttc    2400

tgaaatttcc acctccttgt ttgttttgag agcttgttct gaagcccaat cctacttgga    2460

ttctaaagct tgtgctttcc cagaatctta cccatcttaa gcgaatttct tatgatttat    2520

gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc    2580

ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct    2640

ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa    2700

atgcctgcaa atcgctcccc atttcaccca attgtagata tgctaactcc agcaatgagt    2760

tgatgaatct cggtgtgtat tttatgtcct cagaggacaa cacctgttgt aatcgttctt    2820

ccacac                                                               2826


