                             LISTA DE SECUENCIAS                              

<110> Centro de Ingeniera Gentica y Biotecnologa

<120> POLIPEPTIDOS QUE COMPRENDEN MUTANTES DEL VEGF-A HUMANO
      CON RE-ARREGLOS DE PUENTES DISULFURO Y COMPOSICIONES
      QUE LOS CONTIENEN

<130> VEGFII

<140> CU 2019-0111
<141> 24.12.2019

<160> 24                            

<170> PatentIn Ver. 2.1

<210> 1
<211> 573
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:  acido
      nucleico que codifica para polipeptido-mutante

<400> 1
atggtagata aaagaatggc tttagttgaa ttgaaagtgc ccgacattgg cggacacgaa 60    
aatgtagata ttatcgcggt tgaagtaaac gtgggcgaca ctattgctgt ggacgatacc 120   
ctgattactt tggatctaga tcacgatgac gatgacgata aagcttcaga tctgctagcc 180   
gcacccatgg caaaaggagg agggcagaat catcacgaag tggtgaagtt catggatgtc 240   
tatcagcgcm gtkactgcca tccaatcgag accctggtgg acatcttcca ggagtaccct 300   
gatgagatcg agtacatctt caagccatcc tgtgtgcccc tgatgcgatg cgggggctgc 360   
tgcaatgacg agggcctgga gtgtgtgccc actgaggagt ccaacatcac catgcagatt 420   
atggagatcg aacctgagca aggccagcac ataggagaga tgagcttcct acagcacaac 480   
aaatgtgaat gcagaccaaa gaaagataga gccagacaag aaaaatgtga caagccgagg 540   
cggggatccc gggcacacca tcaccatcac cat                              573   


<210> 2
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      polipeptido-mutante

<400> 2
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 3
<211> 214
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      polipeptido-mutante isoforma 145

<400> 3
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser  
                165                 170                 175    

Val Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg  
            180                 185                 190        

Tyr Lys Ser Trp Ser Val Cys Asp Lys Pro Arg Arg Gly Ser Arg Ala  
        195                 200                 205            

His His His His His His                                          
    210                


<210> 4
<211> 234
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      polipeptido-mutante isoforma 165

<400> 4
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro Cys  
                165                 170                 175    

Gly Pro Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln  
            180                 185                 190        

Thr Cys Lys Cys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg  
        195                 200                 205            

Gln Leu Glu Leu Asn Glu Arg Thr Cys Arg Cys Asp Lys Pro Arg Arg  
    210                 215                 220                

Gly Ser Arg Ala His His His His His His                          
225                 230                


<210> 5
<211> 258
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      polipeptido-mutante isoforma 189

<400> 5
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser  
                165                 170                 175    

Val Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg  
            180                 185                 190        

Tyr Lys Ser Trp Ser Val Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys  
        195                 200                 205            

His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn  
    210                 215                 220                

Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr  
225                 230                 235                 240

Cys Arg Cys Asp Lys Pro Arg Arg Gly Ser Arg Ala His His His His  
                245                 250                 255    

His His                                                          
       


<210> 6
<211> 275
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      Polipeptido-mutante isoforma 206

<400> 6
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser  
                165                 170                 175    

Val Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg  
            180                 185                 190        

Tyr Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro  
        195                 200                 205            

Trp Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg  
    210                 215                 220                

Lys His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys  
225                 230                 235                 240

Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg  
                245                 250                 255    

Thr Cys Arg Cys Asp Lys Pro Arg Arg Gly Ser Arg Ala His His His  
            260                 265                 270        

His His His                                                      
        275


<210> 7
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      oligonucleotido

<400> 7
attgcatgcg cccccgcatc gcatcagggg cactgcgga                        39    


<210> 8
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 8
tccgcagtgc ccctgatgcg atgcgggggc gcatgcaat                        39    


<210> 9
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 9
attgcatgcg cccccgcatc gcatcagggg cacacagga                        39    


<210> 10
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 10
tcctgtgtgc ccctgatgcg atgcgggggc gcatgcaat                        39    


<210> 11
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 11
attgcagcag cccccgcatc gcatcagggg cactcggga                        39    


<210> 12
<211> 39
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 12
tccgcagtgc ccctgatgcg atgcgggggc tgctgcaat                        39    


<210> 13
<211> 24
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 13
ctgctagccg cacccatggc agaa                                        24    


<210> 14
<211> 21
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:oligonucleotido

<400> 14
ccgggatccc cgcctcggct t                                           21    


<210> 15
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante Cys 110 cambio a
      Ala

<400> 15
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Ala Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 16
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante Cys 119 cambio a
      Ala

<400> 16
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Ala Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 17
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante Cys 110 y 119
      cambio a Ala

<400> 17
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Ala Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Ala Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 18
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 1

<220> 
<221> DISULFID
<222> (85)..(110)

<220> 
<221> DISULFID
<222> (116)..(119)

<220> 
<221> DISULFID
<222> (120)..(127)

<220> 
<223> Cys 161, Cys 163 y Cys 175 estan involucradas en
      puentes disulfuro intermoleculares

<400> 18
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 19
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 2

<220> 
<221> DISULFID
<222> (85)..(110)

<220> 
<221> DISULFID
<222> (116)..(120)

<220> 
<221> DISULFID
<222> (119)..(127)

<220> 
<223> Cys 161, Cys 163 y Cys 175 estan involucradas en
      puentes disulfuro intermoleculares

<400> 19
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 20
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 3

<220> 
<221> DISULFID
<222> (85)..(110)

<220> 
<221> DISULFID
<222> (116)..(127)

<220> 
<221> DISULFID
<222> (119)..(120)

<220> 
<223> Cys 161, Cys 163 y Cys 175 estan involucradas en
      puentes disulfuro intermoleculares

<400> 20
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 21
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 4

<220> 
<221> DISULFID
<222> (85)..(175)

<220> 
<221> DISULFID
<222> (110)..(116)

<220> 
<221> DISULFID
<222> (119)..(120)

<220> 
<223> Cys 161 y Cys 163 estan involucradas en puentes
      disulfuro intermoleculares

<400> 21
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 22
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 5

<220> 
<221> DISULFID
<222> (85)..(175)

<220> 
<221> DISULFID
<222> (110)..(119)

<220> 
<221> DISULFID
<222> (116)..(120)

<220> 
<223> Cys 161 y Cys 163 estan involucradas en puentes
      disulfuro intermoleculares

<400> 22
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 23
<211> 190
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia
      Artificial:polipeptido-mutante re-arreglo 6

<220> 
<221> DISULFID
<222> (85)..(175)

<220> 
<221> DISULFID
<222> (110)..(120)

<220> 
<221> DISULFID
<222> (116)..(119)

<220> 
<223> Cys 161 y Cys 163 estan involucradas en puentes
      disulfuro intermoleculares

<400> 23
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp His Asp  
         35                  40                  45            

Asp Asp Asp Asp Lys Ala Ser Asp Leu Leu Ala Ala Pro Met Ala Glu  
     50                  55                  60                

Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr  
 65                  70                  75                  80

Gln Arg Ser Asn Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln  
                 85                  90                  95    

Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro  
            100                 105                 110        

Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val  
        115                 120                 125            

Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Glu Ile Glu Pro  
    130                 135                 140                

Glu Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys  
145                 150                 155                 160

Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Cys Asp  
                165                 170                 175    

Lys Pro Arg Arg Gly Ser Arg Ala His His His His His His          
            180                 185                 190


<210> 24
<211> 46
<212> PRT
<213> Neisseria meningitidis

<400> 24
Val Asp Lys Arg Met Ala Leu Val Glu Leu Lys Val Pro Asp Ile Gly  
  1               5                  10                  15    

Gly His Glu Asn Val Asp Ile Ile Ala Val Glu Val Asn Val Gly Asp  
             20                  25                  30        

Thr Ile Ala Val Asp Asp Thr Leu Ile Thr Leu Asp Leu Asp          
         35                  40                  45    




Dr. C Sonia Gonzlez Blanco
Representante Legal, CIGB

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