                         SEQUENCE LISTING

<110>  UCL Business Ltd
 
<120>  TREATMENT OF CHRONIC PAIN

<130>  N417555WO

<150>  GB 1918879.6
<151>  2019-12-19

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  394
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Advillin consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Position 1 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Position 2 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Position 3 is G, A or no nucleotide

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Position 4 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Position 5 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Position 6 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  Position 57 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (73)..(73)
<223>  Position 73 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (74)..(74)
<223>  Position 74 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (75)..(75)
<223>  Position 75 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Position 76 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  Position 77 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (78)..(78)
<223>  Position 78 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  Position 79 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  Position 80 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (100)..(100)
<223>  Position 100 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (101)..(101)
<223>  Position 101 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (121)..(121)
<223>  Position 121 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Position 150 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (151)..(151)
<223>  Position 151 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (152)..(152)
<223>  Position 152 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (153)..(153)
<223>  Position 153 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (154)..(154)
<223>  Position 154 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (155)..(155)
<223>  Position 155 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (156)..(156)
<223>  Position 156 is A or no nucleotide

<220>
<221>  misc_feature
<222>  (184)..(184)
<223>  Position 184 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (188)..(188)
<223>  Position 188 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Position 222 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (223)..(223)
<223>  Position 223 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (330)..(330)
<223>  Position 330 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (331)..(331)
<223>  Position 331 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (332)..(332)
<223>  Position 332 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (333)..(333)
<223>  Position 333 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (334)..(334)
<223>  Position 334 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (351)..(351)
<223>  Position 351 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (371)..(371)
<223>  Position 371 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (372)..(372)
<223>  Position 372 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (373)..(373)
<223>  Position 373 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (374)..(374)
<223>  Position 374 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (375)..(375)
<223>  Position 375 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (376)..(376)
<223>  Position 376 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (377)..(377)
<223>  Position 377 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (378)..(378)
<223>  Position 378 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (379)..(379)
<223>  Position 379 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (380)..(380)
<223>  Position 380 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (381)..(381)
<223>  Position 381 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (382)..(382)
<223>  Position 382 is G, T or no nucleotide

<220>
<221>  misc_feature
<222>  (383)..(383)
<223>  Position 383 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (384)..(384)
<223>  Position 384 is A, G or no nucleotide

<220>
<221>  misc_feature
<222>  (385)..(385)
<223>  Position 385 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (386)..(386)
<223>  Position 386 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (387)..(387)
<223>  Position 387 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (388)..(388)
<223>  Position 388 is G or no nucleotide

<220>
<221>  misc_feature
<222>  (389)..(389)
<223>  Position 389 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (390)..(390)
<223>  Position 390 is T or no nucleotide

<220>
<221>  misc_feature
<222>  (391)..(391)
<223>  Position 391 is C or no nucleotide

<220>
<221>  misc_feature
<222>  (392)..(392)
<223>  Position 392 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (393)..(393)
<223>  Position 393 is C, T or no nucleotide

<220>
<221>  misc_feature
<222>  (394)..(394)
<223>  Position 394 is C, T or no nucleotide

<400>  1
nnnnnnycwk yctcctgtca tcasyrtaat ryrtyyayat ctmcaatatg gcaaatntca       60

tatcctyccw mmnnnnnnnn ctgmattatt gatggvcwrn ntgcactttt waaaaaatca      120

nwtagaycag ggygtggarc tggartyman nnnnnnrrga rgcctttara agtcygcwct      180

tctngttnyy gctgttttgm ataggmrcag ataaagctty cnnctctggt ttgaataagy      240

caagcycagk gctaggttgg cystgattgg ycagvrctrr garaatkhgg ttawgatgca      300

aacmcaagca aatataaccc dgwrtctvwn nnnngcrgcc attrctrarm nmaggcwkca      360

ggasskgvhg nnnnnnnnnn nnnnnnnnnn nnnn                                  394


<210>  2
<211>  378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Advillin promoter fragment

<400>  2
ccagcctctt cctcctgtca tcaccataat gcatccatat ctacaatatg gcaaatttca       60

tatccttcca acctctttcc tgcattattg atgggctgtg tgcacttttt aaaaaatcaa      120

ttagatcagg gcgtggagct ggagttcaaa gaagccttta aaagtctgct cttctgtttt      180

gctgttttga ataggcacag ataaagcttt ccctctggtt tgaataagcc aagctcagtg      240

ctaggttggc tctgattggc caggactagg aaaatgcggt taagatgcaa acacaagcaa      300

atataaccca gtatctctgc ggccattact aagctaaggc agcaggacct ggagcctcct      360

gctttggagt ggttcttc                                                    378


<210>  3
<211>  344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Advillin promoter fragment

<400>  3
ccagtctcct gtcatcagca taatacgttc atatctacaa tatggcaaat tcatatcctc       60

cctcactgaa ttattgatgg acagtgcact tttaaaaaaa tcaatagacc agggtgtgga      120

gctggagtta aaagaagcct ttagaagtct gctcttcttg ttttcgctgt tttgcatagg      180

agcagataaa gctttcctct ctggtttgaa taagtcaagc ccagggctag gttggctgtg      240

attggccaga gctaggagaa tgtggttatg atgcaaacac aagcaaatat aaccctgtgt      300

ctatgctgtg cggccattgc tgaaacaggc ttcaggacgg gctg                       344


<210>  4
<211>  376
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pig Advillin promoter fragment

<400>  4
ccagcccctg cctcctgtca tcagtgtaat gtgtctacat ctccaatatg gcaaatttca       60

tatcctccca ccctctttct gcattattga tggcctgtgc acttttaaaa aaatcaatta      120

gatcagggcg tggagctgga gttaaaagaa gcctttaaaa gtctgcactt cttgttttgc      180

tgttttgaat aggaacagat aaagctttcc ctctggtttg aataagtcaa gcccagggct      240

aggttggctg tgattggtca ggactaagaa aatgcggtta agatgcaaac ccaagcaaat      300

ataacccagt gtctgagcag ccattactga gctaaggcag caggaccgga agcctcccgc      360

ttttgggtgg ttcctc                                                      376


<210>  5
<211>  386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dog Advillin promoter fragment

<400>  5
ccggcccctg cctcctgtca tcagcataat gcatccatat ctacaatatg gcaaatttca       60

tatcctccca ccctctttcc ctgcattatt gatgggctat gcacttttaa aaaaatcaat      120

tagatcaggg tgtggaactg gaatcaaaag aaaagggagg cctttaaaag tccgctcttc      180

ttgtttctgc tgttttgaat aggagcagat aaagcttccc ctctggtttg aataagtcaa      240

gcccagggct aggttggccg tgattggcca gcactgggaa aattaggtta agatgcaaac      300

acaagcaaat ataacccgga gtctgtgcag ccattgctaa gctaaggcag caggagctgg      360

cgcctcccgc cccggagtgg ttctct                                           386


<210>  6
<211>  1298
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Advillin larger promoter region (including exon 1)

<400>  6
tgtgagctgc caccaacctt cattggccct taagtgttca ctgggcccat ggcaagcacc       60

gcctttcttc tcgcaacccc ttccatcatg aagcagctgc acccccgggc tgagtggggt      120

gttgcaccca ccgggaggcc agctgcagat gccacacagg gcttccgctc aaggttacac      180

aagttctgct gtgatcaacc ccattgcagt caggtgctta gcacgtgtat atgcttgatg      240

tcctattgac ttctgggcat gaaccagaag ccctaaggga atggattatt attttttttt      300

ttttttttga aatggagtct tgctctgttg cccaggctag aggcagtggc atgatctcag      360

ctcactgcaa cctccacctc ccaggttcaa gcgattctcc tgcctcagtc tcctgagtag      420

ctgggattac aggcgcctgc cagcacgccc ggctcatttt tgtattttta gtagagatgg      480

ggtttcacca tcttggccag gctgttttca aactcctcac attgtgatcc acccacctca      540

gcctcccaaa gtgctgggat tacaggcgtg agccaccacc acacccagcc ctgggaatgg      600

agtcttgatt cttctctgcc cctcactgat tcttccaaac tggaaaccct gaagctgaga      660

gcccagcatg gttcctggca aacagcaggc actcaaatat tgattggttt actgtatgac      720

tagtagagac cccaacgagc aaaactgtgg cctaataaaa ttctggctcc tctcccagac      780

ttcccctccc tttgagaaat gccagaagct tcttagggag gctcttgcca acctagacat      840

cacaggcact catggggcag ctccagcctc ttcctcctgt catcaccata atgcatccat      900

atctacaata tggcaaattt catatccttc caacctcttt cctgcattat tgatgggctg      960

tgtgcacttt ttaaaaaatc aattagatca gggcgtggag ctggagttca aagaagcctt     1020

taaaagtctg ctcttctgtt ttgctgtttt gaataggcac agataaagct ttccctctgg     1080

tttgaataag ccaagctcag tgctaggttg gctctgattg gccaggacta ggaaaatgcg     1140

gttaagatgc aaacacaagc aaatataacc cagtatctct gcggccatta ctaagctaag     1200

gcagcaggac ctggagcctc ctgctttgga gtggttcttc aatactgctg ctgcttacgc     1260

gccgggaaac tgggaaggct ggtgagcgag aaggcaag                             1298


<210>  7
<211>  856
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Advillin larger promoter region (including exon 1)

<400>  7
actgggagct gcttttgctt tccttccctt ccactgacct gacactcggt ggacactgtt       60

cttcctgcat gacctcgttg atcgtgaaat gatagcacct ggcagagcaa acgctgggat      120

tctgatcaag gttacagagg ttggagtgca ttagcccctc tcgggcctgg gttggaacca      180

actccctctc cccaggtact tggcaagcgt ttgcttgctg tgctattgac tggggtagag      240

actgaagccc tgggggaatt ggaggctcct gcatgtgctc ttgatgctgc caaacaggcc      300

ttgagattca gagcccagca tggtttccag gcacatagta ggatgacttg tacagactcc      360

aagcaacaaa agctttcctg acaccacaga attgcccaga atcatctctc agccctgaac      420

gtccaggcac acagtagggg agctctagcc agtctcctgt catcagcata atacgttcat      480

atctacaata tggcaaattc atatcctccc tcactgaatt attgatggac agtgcacttt      540

taaaaaaatc aatagaccag ggtgtggagc tggagttaaa agaagccttt agaagtctgc      600

tcttcttgtt ttcgctgttt tgcataggag cagataaagc tttcctctct ggtttgaata      660

agtcaagccc agggctaggt tggctgtgat tggccagagc taggagaatg tggttatgat      720

gcaaacacaa gcaaatataa ccctgtgtct atgctgtgcg gccattgctg aaacaggctt      780

caggacgggc tgctttggga gacctcatca gtacttcacc tgctgctttg ccggacttcg      840

gaggacagag ggtcag                                                      856


<210>  8
<211>  937
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pig Advillin larger promoter region (including exon 1)

<400>  8
atcctgctgt tgttcatttg ggcacttaac cccttcttcc ctcaaaagcc ctctgtggtg       60

atgaatgatg aagccagagc gcccaggctg ggaggccagc tacggactca gcacaggggt      120

tgtgctccag gttacagaag tctggctatg ttagctcttc cctggcctcc atgtggaacc      180

aagcccagtg ccatccaggt gcttaataag catatgcttg gggtcttaac tgacttctag      240

ggaggaacca gaagccccaa gggacttgga ctcttctttc ttctgtgctc ctcactgatt      300

ttcccaaact aggagccctg aaactcagcc cagggtagtt cctggtactt agtaggcact      360

gagatactga ttggatggtt ttgcccaagg ggcaaaacag gatgatgtaa taacatactc      420

ctggcccctc tcccagactg cccctccgag aaatgcccag ctgggagggg cttgccagcc      480

tggacatccc aggtgctcag ggggcagccc cagcccctgc ctcctgtcat cagtgtaatg      540

tgtctacatc tccaatatgg caaatttcat atcctcccac cctctttctg cattattgat      600

ggcctgtgca cttttaaaaa aatcaattag atcagggcgt ggagctggag ttaaaagaag      660

cctttaaaag tctgcacttc ttgttttgct gttttgaata ggaacagata aagctttccc      720

tctggtttga ataagtcaag cccagggcta ggttggctgt gattggtcag gactaagaaa      780

atgcggttaa gatgcaaacc caagcaaata taacccagtg tctgagcagc cattactgag      840

ctaaggcagc aggaccggaa gcctcccgct tttgggtggt tcctcaacac taattatccg      900

ccaaaaaact gggaagactg gtgagagaga aaacaag                               937


<210>  9
<211>  974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dog Advillin larger promoter region (including exon 1)

<400>  9
ccctggtgtc gttcattcgg gagtcaccgg cacactcttc ttcccgtgca tccaacccct       60

tgtgtcacgg cagagctgcg cacccaggct gggcaggctg ttgctccggc cgggaggcca      120

gctacggact cggctcggct tccgctcaag gttacagaag ttgcactaca tgagttctgt      180

tctggccccc cgtgtggaag cagctccgtt gccgtccagg tgcttgataa gcatacgctt      240

ggtgtcccat tgacttctgg gaatgaacca ggagccccga gggagtagga gtcttctgtg      300

ccccttactg cttctcccag actgggatcc ccggaaccta gaatccatga ttcttggcac      360

atagtaggca ctcagattct ggttggactg ttgtatggac cccaagggaa gccctaaaga      420

gcaaaacagg atgacctaat ataacacatc ctggcccctc ccctagacct ctctgcctct      480

gagaaatacc cagaagcttc tcagggaggc acttgccagc ccaggcactc aggggcagct      540

ccggcccctg cctcctgtca tcagcataat gcatccatat ctacaatatg gcaaatttca      600

tatcctccca ccctctttcc ctgcattatt gatgggctat gcacttttaa aaaaatcaat      660

tagatcaggg tgtggaactg gaatcaaaag aaaagggagg cctttaaaag tccgctcttc      720

ttgtttctgc tgttttgaat aggagcagat aaagcttccc ctctggtttg aataagtcaa      780

gcccagggct aggttggccg tgattggcca gcactgggaa aattaggtta agatgcaaac      840

acaagcaaat ataacccgga gtctgtgcag ccattgctaa gctaaggcag caggagctgg      900

cgcctcccgc cccggagtgg ttctctggat accgcctgga aacccggtgg gagcccggcc      960

ggagagcagg caag                                                        974


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scn9a guide sequence 1

<400>  10
ccactttatg tggcttgaag g                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scn9a guide sequence 2

<400>  11
aaggagagag ctgagtcagg t                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scn9a guide sequence 3

<400>  12
tcctcgatgc tccctgagct c                                                 21


<210>  13
<211>  3156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9

<400>  13
aagcggaact acatcctggg cctggacatc ggcatcacca gcgtgggcta cggcatcatc       60

gactacgaga cacgggacgt gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg      120

gaaaacaacg agggcaggcg gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg      180

catagaatcc agagagtgaa gaagctgctg ttcgactaca acctgctgac cgaccacagc      240

gagctgagcg gcatcaaccc ctacgaggcc agagtgaagg gcctgagcca gaagctgagc      300

gaggaagagt tctctgccgc cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg      360

aacgaggtgg aagaggacac cggcaacgag ctgtccacca aagagcagat cagccggaac      420

agcaaggccc tggaagagaa atacgtggcc gaactgcagc tggaacggct gaagaaagac      480

ggcgaagtgc ggggcagcat caacagattc aagaccagcg actacgtgaa agaagccaaa      540

cagctgctga aggtgcagaa ggcctaccac cagctggacc agagcttcat cgacacctac      600

atcgacctgc tggaaacccg gcggacctac tatgagggac ctggcgaggg cagccccttc      660

ggctggaagg acatcaaaga atggtacgag atgctgatgg gccactgcac ctacttcccc      720

gaggaactgc ggagcgtgaa gtacgcctac aacgccgacc tgtacaacgc cctgaacgac      780

ctgaacaatc tcgtgatcac cagggacgag aacgagaagc tggaatatta cgagaagttc      840

cagatcatcg agaacgtgtt caagcagaag aagaagccca ccctgaagca gatcgccaaa      900

gaaatcctcg tgaacgaaga ggatattaag ggctacagag tgaccagcac cggcaagccc      960

gagttcacca acctgaaggt gtaccacgac atcaaggaca ttaccgcccg gaaagagatt     1020

attgagaacg ccgagctgct ggatcagatt gccaagatcc tgaccatcta ccagagcagc     1080

gaggacatcc aggaagaact gaccaatctg aactccgagc tgacccagga agagatcgag     1140

cagatctcta atctgaaggg ctataccggc acccacaacc tgagcctgaa ggccatcaac     1200

ctgatcctgg acgagctgtg gcacaccaac gacaaccaga tcgctatctt caaccggctg     1260

aagctggtgc ccaagaaggt ggacctgtcc cagcagaaag agatccccac caccctggtg     1320

gacgacttca tcctgagccc cgtcgtgaag agaagcttca tccagagcat caaagtgatc     1380

aacgccatca tcaagaagta cggcctgccc aacgacatca ttatcgagct ggcccgcgag     1440

aagaactcca aggacgccca gaaaatgatc aacgagatgc agaagcggaa ccggcagacc     1500

aacgagcgga tcgaggaaat catccggacc accggcaaag agaacgccaa gtacctgatc     1560

gagaagatca agctgcacga catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc     1620

cctctggaag atctgctgaa caaccccttc aactatgagg tggaccacat catccccaga     1680

agcgtgtcct tcgacaacag cttcaacaac aaggtgctcg tgaagcagga agaaaacagc     1740

aagaagggca accggacccc attccagtac ctgagcagca gcgacagcaa gatcagctac     1800

gaaaccttca agaagcacat cctgaatctg gccaagggca agggcagaat cagcaagacc     1860

aagaaagagt atctgctgga agaacgggac atcaacaggt tctccgtgca gaaagacttc     1920

atcaaccgga acctggtgga taccagatac gccaccagag gcctgatgaa cctgctgcgg     1980

agctacttca gagtgaacaa cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc     2040

agctttctgc ggcggaagtg gaagtttaag aaagagcgga acaaggggta caagcaccac     2100

gccgaggacg ccctgatcat tgccaacgcc gatttcatct tcaaagagtg gaagaaactg     2160

gacaaggcca aaaaagtgat ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg     2220

cccgagatcg aaaccgagca ggagtacaaa gagatcttca tcacccccca ccagatcaag     2280

cacattaagg acttcaagga ctacaagtac agccaccggg tggacaagaa gcctaataga     2340

gagctgatta acgacaccct gtactccacc cggaaggacg acaagggcaa caccctgatc     2400

gtgaacaatc tgaacggcct gtacgacaag gacaatgaca agctgaaaaa gctgatcaac     2460

aagagccccg aaaagctgct gatgtaccac cacgaccccc agacctacca gaaactgaag     2520

ctgattatgg aacagtacgg cgacgagaag aatcccctgt acaagtacta cgaggaaacc     2580

gggaactacc tgaccaagta ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag     2640

tattacggca acaaactgaa cgcccatctg gacatcaccg acgactaccc caacagcaga     2700

aacaaggtcg tgaagctgtc cctgaagccc tacagattcg acgtgtacct ggacaatggc     2760

gtgtacaagt tcgtgaccgt gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa     2820

gtgaatagca agtgctatga ggaagctaag aagctgaaga agatcagcaa ccaggccgag     2880

tttatcgcct ccttctacaa caacgatctg atcaagatca acggcgagct gtatagagtg     2940

atcggcgtga acaacgacct gctgaaccgg atcgaagtga acatgatcga catcacctac     3000

cgcgagtacc tggaaaacat gaacgacaag aggcccccca ggatcattaa gacaatcgcc     3060

tccaagaccc agagcattaa gaagtacagc acagacattc tgggcaacct gtatgaagtg     3120

aaatctaaga agcaccctca gatcatcaaa aagggc                               3156


<210>  14
<211>  3156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dSaCas9

<400>  14
aagcggaact acatcctggg cctggccatc ggcatcacca gcgtgggcta cggcatcatc       60

gactacgaga cacgggacgt gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg      120

gaaaacaacg agggcaggcg gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg      180

catagaatcc agagagtgaa gaagctgctg ttcgactaca acctgctgac cgaccacagc      240

gagctgagcg gcatcaaccc ctacgaggcc agagtgaagg gcctgagcca gaagctgagc      300

gaggaagagt tctctgccgc cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg      360

aacgaggtgg aagaggacac cggcaacgag ctgtccacca aagagcagat cagccggaac      420

agcaaggccc tggaagagaa atacgtggcc gaactgcagc tggaacggct gaagaaagac      480

ggcgaagtgc ggggcagcat caacagattc aagaccagcg actacgtgaa agaagccaaa      540

cagctgctga aggtgcagaa ggcctaccac cagctggacc agagcttcat cgacacctac      600

atcgacctgc tggaaacccg gcggacctac tatgagggac ctggcgaggg cagccccttc      660

ggctggaagg acatcaaaga atggtacgag atgctgatgg gccactgcac ctacttcccc      720

gaggaactgc ggagcgtgaa gtacgcctac aacgccgacc tgtacaacgc cctgaacgac      780

ctgaacaatc tcgtgatcac cagggacgag aacgagaagc tggaatatta cgagaagttc      840

cagatcatcg agaacgtgtt caagcagaag aagaagccca ccctgaagca gatcgccaaa      900

gaaatcctcg tgaacgaaga ggatattaag ggctacagag tgaccagcac cggcaagccc      960

gagttcacca acctgaaggt gtaccacgac atcaaggaca ttaccgcccg gaaagagatt     1020

attgagaacg ccgagctgct ggatcagatt gccaagatcc tgaccatcta ccagagcagc     1080

gaggacatcc aggaagaact gaccaatctg aactccgagc tgacccagga agagatcgag     1140

cagatctcta atctgaaggg ctataccggc acccacaacc tgagcctgaa ggccatcaac     1200

ctgatcctgg acgagctgtg gcacaccaac gacaaccaga tcgctatctt caaccggctg     1260

aagctggtgc ccaagaaggt ggacctgtcc cagcagaaag agatccccac caccctggtg     1320

gacgacttca tcctgagccc cgtcgtgaag agaagcttca tccagagcat caaagtgatc     1380

aacgccatca tcaagaagta cggcctgccc aacgacatca ttatcgagct ggcccgcgag     1440

aagaactcca aggacgccca gaaaatgatc aacgagatgc agaagcggaa ccggcagacc     1500

aacgagcgga tcgaggaaat catccggacc accggcaaag agaacgccaa gtacctgatc     1560

gagaagatca agctgcacga catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc     1620

cctctggaag atctgctgaa caaccccttc aactatgagg tggaccacat catccccaga     1680

agcgtgtcct tcgacaacag cttcaacaac aaggtgctcg tgaagcagga agaagccagc     1740

aagaagggca accggacccc attccagtac ctgagcagca gcgacagcaa gatcagctac     1800

gaaaccttca agaagcacat cctgaatctg gccaagggca agggcagaat cagcaagacc     1860

aagaaagagt atctgctgga agaacgggac atcaacaggt tctccgtgca gaaagacttc     1920

atcaaccgga acctggtgga taccagatac gccaccagag gcctgatgaa cctgctgcgg     1980

agctacttca gagtgaacaa cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc     2040

agctttctgc ggcggaagtg gaagtttaag aaagagcgga acaaggggta caagcaccac     2100

gccgaggacg ccctgatcat tgccaacgcc gatttcatct tcaaagagtg gaagaaactg     2160

gacaaggcca aaaaagtgat ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg     2220

cccgagatcg aaaccgagca ggagtacaaa gagatcttca tcacccccca ccagatcaag     2280

cacattaagg acttcaagga ctacaagtac agccaccggg tggacaagaa gcctaataga     2340

gagctgatta acgacaccct gtactccacc cggaaggacg acaagggcaa caccctgatc     2400

gtgaacaatc tgaacggcct gtacgacaag gacaatgaca agctgaaaaa gctgatcaac     2460

aagagccccg aaaagctgct gatgtaccac cacgaccccc agacctacca gaaactgaag     2520

ctgattatgg aacagtacgg cgacgagaag aatcccctgt acaagtacta cgaggaaacc     2580

gggaactacc tgaccaagta ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag     2640

tattacggca acaaactgaa cgcccatctg gacatcaccg acgactaccc caacagcaga     2700

aacaaggtcg tgaagctgtc cctgaagccc tacagattcg acgtgtacct ggacaatggc     2760

gtgtacaagt tcgtgaccgt gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa     2820

gtgaatagca agtgctatga ggaagctaag aagctgaaga agatcagcaa ccaggccgag     2880

tttatcgcct ccttctacaa caacgatctg atcaagatca acggcgagct gtatagagtg     2940

atcggcgtga acaacgacct gctgaaccgg atcgaagtga acatgatcga catcacctac     3000

cgcgagtacc tggaaaacat gaacgacaag aggcccccca ggatcattaa gacaatcgcc     3060

tccaagaccc agagcattaa gaagtacagc acagacattc tgggcaacct gtatgaagtg     3120

aaatctaaga agcaccctca gatcatcaaa aagggc                               3156


<210>  15
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KRAB domain

<400>  15
cggacactgg tgaccttcaa ggatgtgttt gtggacttca ccagggagga gtggaagctg       60

ctggacactg ctcagcagat cctgtacaga aatgtgatgc tggagaacta taagaacctg      120

gtttccttgg gttatcagct tactaagcca gatgtgatcc tccggttgga gaagggagaa      180

gagccctggc tggtg                                                       195


<210>  16
<211>  3345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NLS-SaCas9-NLS-3xHA

<400>  16
atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc caagcggaac       60

tacatcctgg gcctggacat cggcatcacc agcgtgggct acggcatcat cgactacgag      120

acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag aggccaacgt ggaaaacaac      180

gagggcaggc ggagcaagag aggcgccaga aggctgaagc ggcggaggcg gcatagaatc      240

cagagagtga agaagctgct gttcgactac aacctgctga ccgaccacag cgagctgagc      300

ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc agaagctgag cgaggaagag      360

ttctctgccg ccctgctgca cctggccaag agaagaggcg tgcacaacgt gaacgaggtg      420

gaagaggaca ccggcaacga gctgtccacc aaagagcaga tcagccggaa cagcaaggcc      480

ctggaagaga aatacgtggc cgaactgcag ctggaacggc tgaagaaaga cggcgaagtg      540

cggggcagca tcaacagatt caagaccagc gactacgtga aagaagccaa acagctgctg      600

aaggtgcaga aggcctacca ccagctggac cagagcttca tcgacaccta catcgacctg      660

ctggaaaccc ggcggaccta ctatgaggga cctggcgagg gcagcccctt cggctggaag      720

gacatcaaag aatggtacga gatgctgatg ggccactgca cctacttccc cgaggaactg      780

cggagcgtga agtacgccta caacgccgac ctgtacaacg ccctgaacga cctgaacaat      840

ctcgtgatca ccagggacga gaacgagaag ctggaatatt acgagaagtt ccagatcatc      900

gagaacgtgt tcaagcagaa gaagaagccc accctgaagc agatcgccaa agaaatcctc      960

gtgaacgaag aggatattaa gggctacaga gtgaccagca ccggcaagcc cgagttcacc     1020

aacctgaagg tgtaccacga catcaaggac attaccgccc ggaaagagat tattgagaac     1080

gccgagctgc tggatcagat tgccaagatc ctgaccatct accagagcag cgaggacatc     1140

caggaagaac tgaccaatct gaactccgag ctgacccagg aagagatcga gcagatctct     1200

aatctgaagg gctataccgg cacccacaac ctgagcctga aggccatcaa cctgatcctg     1260

gacgagctgt ggcacaccaa cgacaaccag atcgctatct tcaaccggct gaagctggtg     1320

cccaagaagg tggacctgtc ccagcagaaa gagatcccca ccaccctggt ggacgacttc     1380

atcctgagcc ccgtcgtgaa gagaagcttc atccagagca tcaaagtgat caacgccatc     1440

atcaagaagt acggcctgcc caacgacatc attatcgagc tggcccgcga gaagaactcc     1500

aaggacgccc agaaaatgat caacgagatg cagaagcgga accggcagac caacgagcgg     1560

atcgaggaaa tcatccggac caccggcaaa gagaacgcca agtacctgat cgagaagatc     1620

aagctgcacg acatgcagga aggcaagtgc ctgtacagcc tggaagccat ccctctggaa     1680

gatctgctga acaacccctt caactatgag gtggaccaca tcatccccag aagcgtgtcc     1740

ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg aagaaaacag caagaagggc     1800

aaccggaccc cattccagta cctgagcagc agcgacagca agatcagcta cgaaaccttc     1860

aagaagcaca tcctgaatct ggccaagggc aagggcagaa tcagcaagac caagaaagag     1920

tatctgctgg aagaacggga catcaacagg ttctccgtgc agaaagactt catcaaccgg     1980

aacctggtgg ataccagata cgccaccaga ggcctgatga acctgctgcg gagctacttc     2040

agagtgaaca acctggacgt gaaagtgaag tccatcaatg gcggcttcac cagctttctg     2100

cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt acaagcacca cgccgaggac     2160

gccctgatca ttgccaacgc cgatttcatc ttcaaagagt ggaagaaact ggacaaggcc     2220

aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg ccgagagcat gcccgagatc     2280

gaaaccgagc aggagtacaa agagatcttc atcacccccc accagatcaa gcacattaag     2340

gacttcaagg actacaagta cagccaccgg gtggacaaga agcctaatag agagctgatt     2400

aacgacaccc tgtactccac ccggaaggac gacaagggca acaccctgat cgtgaacaat     2460

ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa agctgatcaa caagagcccc     2520

gaaaagctgc tgatgtacca ccacgacccc cagacctacc agaaactgaa gctgattatg     2580

gaacagtacg gcgacgagaa gaatcccctg tacaagtact acgaggaaac cgggaactac     2640

ctgaccaagt actccaaaaa ggacaacggc cccgtgatca agaagattaa gtattacggc     2700

aacaaactga acgcccatct ggacatcacc gacgactacc ccaacagcag aaacaaggtc     2760

gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc tggacaatgg cgtgtacaag     2820

ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa actactacga agtgaatagc     2880

aagtgctatg aggaagctaa gaagctgaag aagatcagca accaggccga gtttatcgcc     2940

tccttctaca acaacgatct gatcaagatc aacggcgagc tgtatagagt gatcggcgtg     3000

aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg acatcaccta ccgcgagtac     3060

ctggaaaaca tgaacgacaa gaggcccccc aggatcatta agacaatcgc ctccaagacc     3120

cagagcatta agaagtacag cacagacatt ctgggcaacc tgtatgaagt gaaatctaag     3180

aagcaccctc agatcatcaa aaagggcaaa aggccggcgg ccacgaaaaa ggccggccag     3240

gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta cccatacgat     3300

gttccagatt acgcttaccc atacgatgtt ccagattacg cttaa                     3345


<210>  17
<211>  3600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HA-NLS-dSaCas9-NLS-KRAB-NLS

<400>  17
atgtacccat acgatgttcc agattacgct gccccaaaga agaagcggaa ggtcggtatc       60

cacggagtcc cagcagccaa gcggaactac atcctgggcc tggccatcgg catcaccagc      120

gtgggctacg gcatcatcga ctacgagaca cgggacgtga tcgatgccgg cgtgcggctg      180

ttcaaagagg ccaacgtgga aaacaacgag ggcaggcgga gcaagagagg cgccagaagg      240

ctgaagcggc ggaggcggca tagaatccag agagtgaaga agctgctgtt cgactacaac      300

ctgctgaccg accacagcga gctgagcggc atcaacccct acgaggccag agtgaagggc      360

ctgagccaga agctgagcga ggaagagttc tctgccgccc tgctgcacct ggccaagaga      420

agaggcgtgc acaacgtgaa cgaggtggaa gaggacaccg gcaacgagct gtccaccaaa      480

gagcagatca gccggaacag caaggccctg gaagagaaat acgtggccga actgcagctg      540

gaacggctga agaaagacgg cgaagtgcgg ggcagcatca acagattcaa gaccagcgac      600

tacgtgaaag aagccaaaca gctgctgaag gtgcagaagg cctaccacca gctggaccag      660

agcttcatcg acacctacat cgacctgctg gaaacccggc ggacctacta tgagggacct      720

ggcgagggca gccccttcgg ctggaaggac atcaaagaat ggtacgagat gctgatgggc      780

cactgcacct acttccccga ggaactgcgg agcgtgaagt acgcctacaa cgccgacctg      840

tacaacgccc tgaacgacct gaacaatctc gtgatcacca gggacgagaa cgagaagctg      900

gaatattacg agaagttcca gatcatcgag aacgtgttca agcagaagaa gaagcccacc      960

ctgaagcaga tcgccaaaga aatcctcgtg aacgaagagg atattaaggg ctacagagtg     1020

accagcaccg gcaagcccga gttcaccaac ctgaaggtgt accacgacat caaggacatt     1080

accgcccgga aagagattat tgagaacgcc gagctgctgg atcagattgc caagatcctg     1140

accatctacc agagcagcga ggacatccag gaagaactga ccaatctgaa ctccgagctg     1200

acccaggaag agatcgagca gatctctaat ctgaagggct ataccggcac ccacaacctg     1260

agcctgaagg ccatcaacct gatcctggac gagctgtggc acaccaacga caaccagatc     1320

gctatcttca accggctgaa gctggtgccc aagaaggtgg acctgtccca gcagaaagag     1380

atccccacca ccctggtgga cgacttcatc ctgagccccg tcgtgaagag aagcttcatc     1440

cagagcatca aagtgatcaa cgccatcatc aagaagtacg gcctgcccaa cgacatcatt     1500

atcgagctgg cccgcgagaa gaactccaag gacgcccaga aaatgatcaa cgagatgcag     1560

aagcggaacc ggcagaccaa cgagcggatc gaggaaatca tccggaccac cggcaaagag     1620

aacgccaagt acctgatcga gaagatcaag ctgcacgaca tgcaggaagg caagtgcctg     1680

tacagcctgg aagccatccc tctggaagat ctgctgaaca accccttcaa ctatgaggtg     1740

gaccacatca tccccagaag cgtgtccttc gacaacagct tcaacaacaa ggtgctcgtg     1800

aagcaggaag aagccagcaa gaagggcaac cggaccccat tccagtacct gagcagcagc     1860

gacagcaaga tcagctacga aaccttcaag aagcacatcc tgaatctggc caagggcaag     1920

ggcagaatca gcaagaccaa gaaagagtat ctgctggaag aacgggacat caacaggttc     1980

tccgtgcaga aagacttcat caaccggaac ctggtggata ccagatacgc caccagaggc     2040

ctgatgaacc tgctgcggag ctacttcaga gtgaacaacc tggacgtgaa agtgaagtcc     2100

atcaatggcg gcttcaccag ctttctgcgg cggaagtgga agtttaagaa agagcggaac     2160

aaggggtaca agcaccacgc cgaggacgcc ctgatcattg ccaacgccga tttcatcttc     2220

aaagagtgga agaaactgga caaggccaaa aaagtgatgg aaaaccagat gttcgaggaa     2280

aagcaggccg agagcatgcc cgagatcgaa accgagcagg agtacaaaga gatcttcatc     2340

accccccacc agatcaagca cattaaggac ttcaaggact acaagtacag ccaccgggtg     2400

gacaagaagc ctaatagaga gctgattaac gacaccctgt actccacccg gaaggacgac     2460

aagggcaaca ccctgatcgt gaacaatctg aacggcctgt acgacaagga caatgacaag     2520

ctgaaaaagc tgatcaacaa gagccccgaa aagctgctga tgtaccacca cgacccccag     2580

acctaccaga aactgaagct gattatggaa cagtacggcg acgagaagaa tcccctgtac     2640

aagtactacg aggaaaccgg gaactacctg accaagtact ccaaaaagga caacggcccc     2700

gtgatcaaga agattaagta ttacggcaac aaactgaacg cccatctgga catcaccgac     2760

gactacccca acagcagaaa caaggtcgtg aagctgtccc tgaagcccta cagattcgac     2820

gtgtacctgg acaatggcgt gtacaagttc gtgaccgtga agaatctgga tgtgatcaaa     2880

aaagaaaact actacgaagt gaatagcaag tgctatgagg aagctaagaa gctgaagaag     2940

atcagcaacc aggccgagtt tatcgcctcc ttctacaaca acgatctgat caagatcaac     3000

ggcgagctgt atagagtgat cggcgtgaac aacgacctgc tgaaccggat cgaagtgaac     3060

atgatcgaca tcacctaccg cgagtacctg gaaaacatga acgacaagag gccccccagg     3120

atcattaaga caatcgcctc caagacccag agcattaaga agtacagcac agacattctg     3180

ggcaacctgt atgaagtgaa atctaagaag caccctcaga tcatcaaaaa gggcaaaagg     3240

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccga tgctaagtca     3300

ctgactgcct ggtcccggac actggtgacc ttcaaggatg tgtttgtgga cttcaccagg     3360

gaggagtgga agctgctgga cactgctcag cagatcctgt acagaaatgt gatgctggag     3420

aactataaga acctggtttc cttgggttat cagcttacta agccagatgt gatcctccgg     3480

ttggagaagg gagaagagcc ctggctggtg gagagagaaa ttcaccaaga gacccatcct     3540

gattcagaga ctgcatttga aatcaaatca tcagttccga aaaagaaacg caaagtttaa     3600


<210>  18
<211>  232
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human EFS promoter sequence

<400>  18
ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg       60

ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt      120

gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca      180

gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gg              232


<210>  19
<211>  145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse EFS promoter sequence

<400>  19
accggggagc tggcgcgggg caaactggga aagcggtgtc gtgtgctggc tccgccctct       60

tcccgagggt gggggagaac ggtatataag tgcggcagtc gccttggacg ttctttttcg      120

caacgggttt gccgtcagaa cgcag                                            145


