                         SEQUENCE LISTING

<110>  VIB VZW
       VRIJE UNIVERSITEIT BRUSSEL
 
<120>  NANOBODY EXCHANGE CHROMATOGRAPHY

<130>  JaSt/NANEX/676

<150>  EP 19219043.7
<151>  2019-12-20

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA12760 GFP-Nb207 (including C-terminal 6xHis + EPEA tag)

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr Ala 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val 
        35                  40                  45              


Ala Gly Ile Tyr Trp Thr Val Gly Ser Thr Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Arg Gly Phe Thr Leu Ala Pro Thr Arg Ala Asn Glu 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  2
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15818 GFP-NbF103A

<400>  2

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr Ala 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val 
        35                  40                  45              


Ala Gly Ile Tyr Trp Thr Val Gly Ser Thr Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Arg Gly Ala Thr Leu Ala Pro Thr Arg Ala Asn Glu 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  3
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15816 GFP- NbT54A/V55A

<400>  3

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr Ala 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val 
        35                  40                  45              


Ala Gly Ile Tyr Trp Ala Ala Gly Ser Thr Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Arg Gly Phe Thr Leu Ala Pro Thr Arg Ala Asn Glu 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  4
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15861 GFP- NbT54A/V55A/F103A

<400>  4

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Thr Ala 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Phe Val 
        35                  40                  45              


Ala Gly Ile Tyr Trp Ala Ala Gly Ser Thr Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Arg Gly Ala Thr Leu Ala Pro Thr Arg Ala Asn Glu 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  5
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15621 MbNb207cHopQ (C-term. 6xHis)

<400>  5

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr Lys Thr 
1               5                   10                  15      


Thr Thr Ser Val Ile Asp Thr Thr Asn Asp Ala Gln Asn Leu Leu Thr 
            20                  25                  30          


Gln Ala Gln Thr Ile Val Asn Thr Leu Lys Asp Tyr Cys Pro Ile Leu 
        35                  40                  45              


Ile Ala Lys Ser Ser Ser Ser Asn Gly Gly Thr Asn Asn Ala Asn Thr 
    50                  55                  60                  


Pro Ser Trp Gln Thr Ala Gly Gly Gly Lys Asn Ser Cys Ala Thr Phe 
65                  70                  75                  80  


Gly Ala Glu Phe Ser Ala Ala Ser Asp Met Ile Asn Asn Ala Gln Lys 
                85                  90                  95      


Ile Val Gln Glu Thr Gln Gln Leu Ser Ala Asn Gln Pro Lys Asn Ile 
            100                 105                 110         


Thr Gln Pro His Asn Leu Asn Leu Asn Ser Pro Ser Ser Leu Thr Ala 
        115                 120                 125             


Leu Ala Gln Lys Met Leu Lys Asn Ala Gln Ser Gln Ala Glu Ile Leu 
    130                 135                 140                 


Lys Leu Ala Asn Gln Val Glu Ser Asp Phe Asn Lys Leu Ser Ser Gly 
145                 150                 155                 160 


His Leu Lys Asp Tyr Ile Gly Lys Cys Asp Ala Ser Ala Ile Ser Ser 
                165                 170                 175     


Ala Asn Met Thr Met Gln Asn Gln Lys Asn Asn Trp Gly Asn Gly Cys 
            180                 185                 190         


Ala Gly Val Glu Glu Thr Gln Ser Leu Leu Lys Thr Ser Ala Ala Asp 
        195                 200                 205             


Phe Asn Asn Gln Thr Pro Gln Ile Asn Gln Ala Gln Asn Leu Ala Asn 
    210                 215                 220                 


Thr Leu Ile Gln Glu Leu Gly Asn Asn Thr Tyr Glu Gln Leu Ser Arg 
225                 230                 235                 240 


Leu Leu Thr Asn Asp Asn Gly Thr Asn Ser Lys Thr Ser Ala Gln Ala 
                245                 250                 255     


Ile Asn Gln Ala Val Asn Asn Leu Asn Glu Arg Ala Lys Thr Leu Ala 
            260                 265                 270         


Gly Gly Thr Thr Asn Ser Pro Ala Tyr Gln Ala Thr Leu Leu Ala Leu 
        275                 280                 285             


Arg Ser Val Leu Gly Leu Trp Asn Ser Met Gly Tyr Ala Val Ile Cys 
    290                 295                 300                 


Gly Gly Tyr Thr Lys Ser Pro Gly Glu Asn Asn Gln Lys Asp Phe His 
305                 310                 315                 320 


Tyr Thr Asp Glu Asn Gly Asn Gly Thr Thr Ile Asn Cys Gly Gly Ser 
                325                 330                 335     


Thr Asn Ser Asn Gly Thr His Ser Tyr Asn Gly Thr Asn Thr Leu Lys 
            340                 345                 350         


Ala Asp Lys Asn Val Ser Leu Ser Ile Glu Gln Tyr Glu Lys Ile His 
        355                 360                 365             


Glu Ala Tyr Gln Ile Leu Ser Lys Ala Leu Lys Gln Ala Gly Leu Ala 
    370                 375                 380                 


Pro Leu Asn Ser Lys Gly Glu Lys Leu Glu Ala His Val Thr Thr Ser 
385                 390                 395                 400 


Lys Tyr Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe 
                405                 410                 415     


Ser Thr Ala Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg 
            420                 425                 430         


Asp Phe Val Ala Gly Ile Tyr Trp Thr Val Gly Ser Thr Tyr Tyr Ala 
        435                 440                 445             


Asp Ser Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
    450                 455                 460                 


Thr Val Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val 
465                 470                 475                 480 


Tyr Tyr Cys Ala Ala Arg Arg Arg Gly Phe Thr Leu Ala Pro Thr Arg 
                485                 490                 495     


Ala Asn Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            500                 505                 510         


Ser His His His His His His 
        515                 


<210>  6
<211>  905
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15616 MbNb207YgjK (C-term. 6xHis)

<400>  6

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Tyr Lys Glu Glu 
1               5                   10                  15      


Thr Gln Ser Gly Leu Asn Asn Tyr Ala Arg Val Val Glu Lys Gly Gln 
            20                  25                  30          


Tyr Asp Ser Leu Glu Ile Pro Ala Gln Val Ala Ala Ser Trp Glu Ser 
        35                  40                  45              


Gly Arg Asp Asp Ala Ala Val Phe Gly Phe Ile Asp Lys Glu Gln Leu 
    50                  55                  60                  


Asp Lys Tyr Val Ala Asn Gly Gly Lys Arg Ser Asp Trp Thr Val Lys 
65                  70                  75                  80  


Phe Ala Glu Asn Arg Ser Gln Asp Gly Thr Leu Leu Gly Tyr Ser Leu 
                85                  90                  95      


Leu Gln Glu Ser Val Asp Gln Ala Ser Tyr Met Tyr Ser Asp Asn His 
            100                 105                 110         


Tyr Leu Ala Glu Met Ala Thr Ile Leu Gly Lys Pro Glu Glu Ala Lys 
        115                 120                 125             


Arg Tyr Arg Gln Leu Ala Gln Gln Leu Ala Asp Tyr Ile Asn Thr Cys 
    130                 135                 140                 


Met Phe Asp Pro Thr Thr Gln Phe Tyr Tyr Asp Val Arg Ile Glu Asp 
145                 150                 155                 160 


Lys Pro Leu Ala Asn Gly Cys Ala Gly Lys Pro Ile Val Glu Arg Gly 
                165                 170                 175     


Lys Gly Pro Glu Gly Trp Ser Pro Leu Phe Asn Gly Ala Ala Thr Gln 
            180                 185                 190         


Ala Asn Ala Asp Ala Val Val Lys Val Met Leu Asp Pro Lys Glu Phe 
        195                 200                 205             


Asn Thr Phe Val Pro Leu Gly Thr Ala Ala Leu Thr Asn Pro Ala Phe 
    210                 215                 220                 


Gly Ala Asp Ile Tyr Trp Arg Gly Arg Val Trp Val Asp Gln Phe Trp 
225                 230                 235                 240 


Phe Gly Leu Lys Gly Met Glu Arg Tyr Gly Tyr Arg Asp Asp Ala Leu 
                245                 250                 255     


Lys Leu Ala Asp Thr Phe Phe Arg His Ala Lys Gly Leu Thr Ala Asp 
            260                 265                 270         


Gly Pro Ile Gln Glu Asn Tyr Asn Pro Leu Thr Gly Ala Gln Gln Gly 
        275                 280                 285             


Ala Pro Asn Phe Ser Trp Ser Ala Ala His Leu Tyr Met Leu Tyr Asn 
    290                 295                 300                 


Asp Phe Phe Arg Lys Gln Ala Ser Gly Gly Gly Ser Gly Gly Gly Gly 
305                 310                 315                 320 


Ser Gly Gly Gly Gly Ser Gly Asn Ala Asp Asn Tyr Lys Asn Val Ile 
                325                 330                 335     


Asn Arg Thr Gly Ala Pro Gln Tyr Met Lys Asp Tyr Asp Tyr Asp Asp 
            340                 345                 350         


His Gln Arg Phe Asn Pro Phe Phe Asp Leu Gly Ala Trp His Gly His 
        355                 360                 365             


Leu Leu Pro Asp Gly Pro Asn Thr Met Gly Gly Phe Pro Gly Val Ala 
    370                 375                 380                 


Leu Leu Thr Glu Glu Tyr Ile Asn Phe Met Ala Ser Asn Phe Asp Arg 
385                 390                 395                 400 


Leu Thr Val Trp Gln Asp Gly Lys Lys Val Asp Phe Thr Leu Glu Ala 
                405                 410                 415     


Tyr Ser Ile Pro Gly Ala Leu Val Gln Lys Leu Thr Ala Lys Asp Val 
            420                 425                 430         


Gln Val Glu Met Thr Leu Arg Phe Ala Thr Pro Arg Thr Ser Leu Leu 
        435                 440                 445             


Glu Thr Lys Ile Thr Ser Asn Lys Pro Leu Asp Leu Val Trp Asp Gly 
    450                 455                 460                 


Glu Leu Leu Glu Lys Leu Glu Ala Lys Glu Gly Lys Pro Leu Ser Asp 
465                 470                 475                 480 


Lys Thr Ile Ala Gly Glu Tyr Pro Asp Tyr Gln Arg Lys Ile Ser Ala 
                485                 490                 495     


Thr Arg Asp Gly Leu Lys Val Thr Phe Gly Lys Val Arg Ala Thr Trp 
            500                 505                 510         


Asp Leu Leu Thr Ser Gly Glu Ser Glu Tyr Gln Val His Lys Ser Leu 
        515                 520                 525             


Pro Val Gln Thr Glu Ile Asn Gly Asn Arg Phe Thr Ser Lys Ala His 
    530                 535                 540                 


Ile Asn Gly Ser Thr Thr Leu Tyr Thr Thr Tyr Ser His Leu Leu Thr 
545                 550                 555                 560 


Ala Gln Glu Val Ser Lys Glu Gln Met Gln Ile Arg Asp Ile Leu Ala 
                565                 570                 575     


Arg Pro Ala Phe Tyr Leu Thr Ala Ser Gln Gln Arg Trp Glu Glu Tyr 
            580                 585                 590         


Leu Lys Lys Gly Leu Thr Asn Pro Asp Ala Thr Pro Glu Gln Thr Arg 
        595                 600                 605             


Val Ala Val Lys Ala Ile Glu Thr Leu Asn Gly Asn Trp Arg Ser Pro 
    610                 615                 620                 


Gly Gly Ala Val Lys Phe Asn Thr Val Thr Pro Ser Val Thr Gly Arg 
625                 630                 635                 640 


Trp Phe Ser Gly Asn Gln Thr Trp Pro Trp Asp Thr Trp Lys Gln Ala 
                645                 650                 655     


Phe Ala Met Ala His Phe Asn Pro Asp Ile Ala Lys Glu Asn Ile Arg 
            660                 665                 670         


Ala Val Phe Ser Trp Gln Ile Gln Pro Gly Asp Ser Val Arg Pro Gln 
        675                 680                 685             


Asp Val Gly Phe Val Pro Asp Leu Ile Ala Trp Asn Leu Ser Pro Glu 
    690                 695                 700                 


Arg Gly Gly Asp Gly Gly Asn Trp Asn Glu Arg Asn Thr Lys Pro Ser 
705                 710                 715                 720 


Leu Ala Ala Trp Ser Val Met Glu Val Tyr Asn Val Thr Gln Asp Lys 
                725                 730                 735     


Thr Trp Val Ala Glu Met Tyr Pro Lys Leu Val Ala Tyr His Asp Trp 
            740                 745                 750         


Trp Leu Arg Asn Arg Asp His Asn Gly Asn Gly Val Pro Glu Tyr Gly 
        755                 760                 765             


Ala Thr Arg Asp Lys Ala His Asn Thr Glu Ser Gly Glu Met Leu Phe 
    770                 775                 780                 


Thr Val Lys Lys Asp Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg 
785                 790                 795                 800 


Thr Phe Ser Thr Ala Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys 
                805                 810                 815     


Glu Arg Asp Phe Val Ala Gly Ile Tyr Trp Thr Val Gly Ser Thr Tyr 
            820                 825                 830         


Tyr Ala Asp Ser Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
        835                 840                 845             


Lys Asn Thr Val Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr 
    850                 855                 860                 


Ala Val Tyr Tyr Cys Ala Ala Arg Arg Arg Gly Phe Thr Leu Ala Pro 
865                 870                 875                 880 


Thr Arg Ala Asn Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr 
                885                 890                 895     


Val Ser Ser His His His His His His 
            900                 905 


<210>  7
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA4375 EPEA-NbSyn2 (C-term. 6xHis)

<400>  7

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Ser Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Leu Gly Gly Val Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His 
    130         


<210>  8
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA4394 bivalent EPEA-NbSyn2 EPEA (C-term. 6xHis)

<400>  8

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Ser Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Leu Gly Gly Val Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Ser Gln Val Gln Leu Gln Glu Ser Gly Gly 
    130                 135                 140                 


Gly Ser Val Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
145                 150                 155                 160 


Gly Ile Asp Ser Ser Ser Tyr Cys Met Gly Trp Phe Arg Gln Arg Pro 
                165                 170                 175     


Gly Lys Glu Arg Glu Gly Val Ala Arg Ile Asn Gly Leu Gly Gly Val 
            180                 185                 190         


Lys Thr Ala Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg 
        195                 200                 205             


Asp Asn Ala Glu Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro 
    210                 215                 220                 


Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Ala Lys Phe Ser Pro Gly Tyr 
225                 230                 235                 240 


Cys Gly Gly Ser Trp Ser Asn Phe Gly Tyr Trp Gly Gln Gly Thr Gln 
                245                 250                 255     


Val Thr Val Ser Ser His His His His His His 
            260                 265         


<210>  9
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA13016 Synaptojanin-specific Nb (trapper; C-term. 6xHis+EPEA)


<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (115)..(115)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Gly His 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Asn Ser Gly Ile Thr Arg Tyr Ile Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Asn Arg Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Xaa Tyr Cys 
                85                  90                  95      


Ala Arg Asn Arg Gly Ile Val Pro Gly Thr Arg Pro Val Asn Asp Glu 
            100                 105                 110         


Tyr Asp Xaa Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  10
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA13080 Synaptojanin-specific Nb (Stripper; C-term. 6xHis+EPEA)

<400>  10

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Arg Phe Met Phe Asp Asp Tyr 
            20                  25                  30          


Ile Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ser Cys Ile Ser Asn Ser Asp Gly Ser Thr Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Arg Asn Ala Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gln Arg Ala Phe Met Thr Arg Leu Cys Leu Asn Ile Tyr Tyr 
            100                 105                 110         


Ala His Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His 
        115                 120                 125             


His His His Glu Pro Glu Ala 
    130                 135 


<210>  11
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA11138 FIXa-specific Nb (Trapper1; C-term. 6xHis+EPEA)

<400>  11

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Arg Thr Ala Ser Asn Tyr 
            20                  25                  30          


Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Phe Ile Ser Arg Thr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Gly Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Thr Met Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ser Leu Tyr Ile Arg Asn Tyr Tyr Glu Gly Thr Asn Tyr Ala 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125             


His His Glu Pro Glu Ala 
    130                 


<210>  12
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA10304 FIXa-specific Nb (Stripper1; C-term. 6xHis+EPEA)

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Glu Arg Thr Phe Ser Gly Thr 
            20                  25                  30          


Val Met Gly Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Asp Ile Ser Trp Ser Gly Ser Ile Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Val Gly Gly Tyr Ser Tyr Ile Met Ser Arg Ser Pro Ala 
            100                 105                 110         


Asp Phe Gly Ser Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His 
        115                 120                 125             


His His His His His Glu Pro Glu Ala 
    130                 135         


<210>  13
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA10502 FIXa-specific Nb (Trapper2; C-term. 6xHis+EPEA)

<400>  13

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ser Arg Val Tyr 
            20                  25                  30          


Gly Val Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Ser Thr Ser Gly Ser Val Thr Asn Val Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Pro His Gly Arg Pro Tyr Gly Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser His His His His His His Glu Pro Glu Ala 
        115                 120                 125         


<210>  14
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA10309 FIXa-specific Nb (Stripper2; C-term. 6xHis+EPEA)

<400>  14

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ile Gly Phe Ser Ile Tyr 
            20                  25                  30          


Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Ala Gly Asn Glu Asp Lys Asp Tyr Val Glu Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Tyr Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Glu Met Ser Gly Leu Lys Phe Glu Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Gly Leu Arg Arg Gly Val Ser Leu Thr Pro Tyr Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro 
        115                 120                 125             


Glu Ala 
    130 


<210>  15
<211>  903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA14208 FIXa-specific Megabody MbNbFIXacHopQ (Stripper2; C-term. 
       6xHis+EPEA)

<400>  15

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Tyr Lys Glu Glu 
1               5                   10                  15      


Thr Gln Ser Gly Leu Asn Asn Tyr Ala Arg Val Val Glu Lys Gly Gln 
            20                  25                  30          


Tyr Asp Ser Leu Glu Ile Pro Ala Gln Val Ala Ala Ser Trp Glu Ser 
        35                  40                  45              


Gly Arg Asp Asp Ala Ala Val Phe Gly Phe Ile Asp Lys Glu Gln Leu 
    50                  55                  60                  


Asp Lys Tyr Val Ala Asn Gly Gly Lys Arg Ser Asp Trp Thr Val Lys 
65                  70                  75                  80  


Phe Ala Glu Asn Arg Ser Gln Asp Gly Thr Leu Leu Gly Tyr Ser Leu 
                85                  90                  95      


Leu Gln Glu Ser Val Asp Gln Ala Ser Tyr Met Tyr Ser Asp Asn His 
            100                 105                 110         


Tyr Leu Ala Glu Met Ala Thr Ile Leu Gly Lys Pro Glu Glu Ala Lys 
        115                 120                 125             


Arg Tyr Arg Gln Leu Ala Gln Gln Leu Ala Asp Tyr Ile Asn Thr Cys 
    130                 135                 140                 


Met Phe Asp Pro Thr Thr Gln Phe Tyr Tyr Asp Val Arg Ile Glu Asp 
145                 150                 155                 160 


Lys Pro Leu Ala Asn Gly Cys Ala Gly Lys Pro Ile Val Glu Arg Gly 
                165                 170                 175     


Lys Gly Pro Glu Gly Trp Ser Pro Leu Phe Asn Gly Ala Ala Thr Gln 
            180                 185                 190         


Ala Asn Ala Asp Ala Val Val Lys Val Met Leu Asp Pro Lys Glu Phe 
        195                 200                 205             


Asn Thr Phe Val Pro Leu Gly Thr Ala Ala Leu Thr Asn Pro Ala Phe 
    210                 215                 220                 


Gly Ala Asp Ile Tyr Trp Arg Gly Arg Val Trp Val Asp Gln Phe Trp 
225                 230                 235                 240 


Phe Gly Leu Lys Gly Met Glu Arg Tyr Gly Tyr Arg Asp Asp Ala Leu 
                245                 250                 255     


Lys Leu Ala Asp Thr Phe Phe Arg His Ala Lys Gly Leu Thr Ala Asp 
            260                 265                 270         


Gly Pro Ile Gln Glu Asn Tyr Asn Pro Leu Thr Gly Ala Gln Gln Gly 
        275                 280                 285             


Ala Pro Asn Phe Ser Trp Ser Ala Ala His Leu Tyr Met Leu Tyr Asn 
    290                 295                 300                 


Asp Phe Phe Arg Lys Gln Ala Ser Gly Gly Gly Ser Gly Gly Gly Gly 
305                 310                 315                 320 


Ser Gly Gly Gly Gly Ser Gly Asn Ala Asp Asn Tyr Lys Asn Val Ile 
                325                 330                 335     


Asn Arg Thr Gly Ala Pro Gln Tyr Met Lys Asp Tyr Asp Tyr Asp Asp 
            340                 345                 350         


His Gln Arg Phe Asn Pro Phe Phe Asp Leu Gly Ala Trp His Gly His 
        355                 360                 365             


Leu Leu Pro Asp Gly Pro Asn Thr Met Gly Gly Phe Pro Gly Val Ala 
    370                 375                 380                 


Leu Leu Thr Glu Glu Tyr Ile Asn Phe Met Ala Ser Asn Phe Asp Arg 
385                 390                 395                 400 


Leu Thr Val Trp Gln Asp Gly Lys Lys Val Asp Phe Thr Leu Glu Ala 
                405                 410                 415     


Tyr Ser Ile Pro Gly Ala Leu Val Gln Lys Leu Thr Ala Lys Asp Val 
            420                 425                 430         


Gln Val Glu Met Thr Leu Arg Phe Ala Thr Pro Arg Thr Ser Leu Leu 
        435                 440                 445             


Glu Thr Lys Ile Thr Ser Asn Lys Pro Leu Asp Leu Val Trp Asp Gly 
    450                 455                 460                 


Glu Leu Leu Glu Lys Leu Glu Ala Lys Glu Gly Lys Pro Leu Ser Asp 
465                 470                 475                 480 


Lys Thr Ile Ala Gly Glu Tyr Pro Asp Tyr Gln Arg Lys Ile Ser Ala 
                485                 490                 495     


Thr Arg Asp Gly Leu Lys Val Thr Phe Gly Lys Val Arg Ala Thr Trp 
            500                 505                 510         


Asp Leu Leu Thr Ser Gly Glu Ser Glu Tyr Gln Val His Lys Ser Leu 
        515                 520                 525             


Pro Val Gln Thr Glu Ile Asn Gly Asn Arg Phe Thr Ser Lys Ala His 
    530                 535                 540                 


Ile Asn Gly Ser Thr Thr Leu Tyr Thr Thr Tyr Ser His Leu Leu Thr 
545                 550                 555                 560 


Ala Gln Glu Val Ser Lys Glu Gln Met Gln Ile Arg Asp Ile Leu Ala 
                565                 570                 575     


Arg Pro Ala Phe Tyr Leu Thr Ala Ser Gln Gln Arg Trp Glu Glu Tyr 
            580                 585                 590         


Leu Lys Lys Gly Leu Thr Asn Pro Asp Ala Thr Pro Glu Gln Thr Arg 
        595                 600                 605             


Val Ala Val Lys Ala Ile Glu Thr Leu Asn Gly Asn Trp Arg Ser Pro 
    610                 615                 620                 


Gly Gly Ala Val Lys Phe Asn Thr Val Thr Pro Ser Val Thr Gly Arg 
625                 630                 635                 640 


Trp Phe Ser Gly Asn Gln Thr Trp Pro Trp Asp Thr Trp Lys Gln Ala 
                645                 650                 655     


Phe Ala Met Ala His Phe Asn Pro Asp Ile Ala Lys Glu Asn Ile Arg 
            660                 665                 670         


Ala Val Phe Ser Trp Gln Ile Gln Pro Gly Asp Ser Val Arg Pro Gln 
        675                 680                 685             


Asp Val Gly Phe Val Pro Asp Leu Ile Ala Trp Asn Leu Ser Pro Glu 
    690                 695                 700                 


Arg Gly Gly Asp Gly Gly Asn Trp Asn Glu Arg Asn Thr Lys Pro Ser 
705                 710                 715                 720 


Leu Ala Ala Trp Ser Val Met Glu Val Tyr Asn Val Thr Gln Asp Lys 
                725                 730                 735     


Thr Trp Val Ala Glu Met Tyr Pro Lys Leu Val Ala Tyr His Asp Trp 
            740                 745                 750         


Trp Leu Arg Asn Arg Asp His Asn Gly Asn Gly Val Pro Glu Tyr Gly 
        755                 760                 765             


Ala Thr Arg Asp Lys Ala His Asn Thr Glu Ser Gly Glu Met Leu Phe 
    770                 775                 780                 


Thr Val Lys Lys Asp Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ile 
785                 790                 795                 800 


Gly Phe Ser Ile Tyr Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys 
                805                 810                 815     


Glu Arg Glu Leu Val Ala Ser Ile Ala Gly Asn Glu Asp Lys Asp Tyr 
            820                 825                 830         


Val Glu Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Tyr Ala Lys 
        835                 840                 845             


Asn Thr Leu Tyr Leu Glu Met Ser Gly Leu Lys Phe Glu Asp Thr Ala 
    850                 855                 860                 


Met Tyr Tyr Cys Ala Ala Gly Leu Arg Arg Gly Val Ser Leu Thr Pro 
865                 870                 875                 880 


Tyr Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His 
                885                 890                 895     


His His His Glu Pro Glu Ala 
            900             


<210>  16
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP protein

<400>  16

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  17
<211>  428
<212>  PRT
<213>  Heliobacter pylori

<400>  17

Met Ala Val Gln Lys Val Lys Asn Ala Asp Lys Val Gln Lys Leu Ser 
1               5                   10                  15      


Asp Thr Tyr Glu Gln Leu Ser Arg Leu Leu Thr Asn Asp Asn Gly Thr 
            20                  25                  30          


Asn Ser Lys Thr Ser Ala Gln Ala Ile Asn Gln Ala Val Asn Asn Leu 
        35                  40                  45              


Asn Glu Arg Ala Lys Thr Leu Ala Gly Gly Thr Thr Asn Ser Pro Ala 
    50                  55                  60                  


Tyr Gln Ala Thr Leu Leu Ala Leu Arg Ser Val Leu Gly Leu Trp Asn 
65                  70                  75                  80  


Ser Met Gly Tyr Ala Val Ile Cys Gly Gly Tyr Thr Lys Ser Pro Gly 
                85                  90                  95      


Glu Asn Asn Gln Lys Asp Phe His Tyr Thr Asp Glu Asn Gly Asn Gly 
            100                 105                 110         


Thr Thr Ile Asn Cys Gly Gly Ser Thr Asn Ser Asn Gly Thr His Ser 
        115                 120                 125             


Tyr Asn Gly Thr Asn Thr Leu Lys Ala Asp Lys Asn Val Ser Leu Ser 
    130                 135                 140                 


Ile Glu Gln Tyr Glu Lys Ile His Glu Ala Tyr Gln Ile Leu Ser Lys 
145                 150                 155                 160 


Ala Leu Lys Gln Ala Gly Leu Ala Pro Leu Asn Ser Lys Gly Glu Lys 
                165                 170                 175     


Leu Glu Ala His Val Thr Thr Ser Lys Tyr Gln Gln Asp Asn Gln Thr 
            180                 185                 190         


Lys Thr Thr Thr Ser Val Ile Asp Thr Thr Asn Asp Ala Gln Asn Leu 
        195                 200                 205             


Leu Thr Gln Ala Gln Thr Ile Val Asn Thr Leu Lys Asp Tyr Cys Pro 
    210                 215                 220                 


Ile Leu Ile Ala Lys Ser Ser Ser Ser Asn Gly Gly Thr Asn Asn Ala 
225                 230                 235                 240 


Asn Thr Pro Ser Trp Gln Thr Ala Gly Gly Gly Lys Asn Ser Cys Ala 
                245                 250                 255     


Thr Phe Gly Ala Glu Phe Ser Ala Ala Ser Asp Met Ile Asn Asn Ala 
            260                 265                 270         


Gln Lys Ile Val Gln Glu Thr Gln Gln Leu Ser Ala Asn Gln Pro Lys 
        275                 280                 285             


Asn Ile Thr Gln Pro His Asn Leu Asn Leu Asn Ser Pro Ser Ser Leu 
    290                 295                 300                 


Thr Ala Leu Ala Gln Lys Met Leu Lys Asn Ala Gln Ser Gln Ala Glu 
305                 310                 315                 320 


Ile Leu Lys Leu Ala Asn Gln Val Glu Ser Asp Phe Asn Lys Leu Ser 
                325                 330                 335     


Ser Gly His Leu Lys Asp Tyr Ile Gly Lys Cys Asp Ala Ser Ala Ile 
            340                 345                 350         


Ser Ser Ala Asn Met Thr Met Gln Asn Gln Lys Asn Asn Trp Gly Asn 
        355                 360                 365             


Gly Cys Ala Gly Val Glu Glu Thr Gln Ser Leu Leu Lys Thr Ser Ala 
    370                 375                 380                 


Ala Asp Phe Asn Asn Gln Thr Pro Gln Ile Asn Gln Ala Gln Asn Leu 
385                 390                 395                 400 


Ala Asn Thr Leu Ile Gln Glu Leu Gly Asn Asn Pro Phe Arg Asn Met 
                405                 410                 415     


Gly Met Ile Ala Ser Ser Thr Thr Asn Asn Gly Ala 
            420                 425             


<210>  18
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16047 GFP-Nb (including C-terminal 6xHis + EPEA tag)

<400>  18

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Gly Tyr 
            20                  25                  30          


Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ser Cys Ile Ser Ser Ser Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Ala Trp Ser Pro Ser Thr Gly Cys Gly Ser Tyr Tyr Val Gln 
            100                 105                 110         


Arg Pro Pro Gly Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
        115                 120                 125             


Ser Ser His His His His His His Glu Pro Glu Ala 
    130                 135                 140 


<210>  19
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16695 GFP-Nb_Y119F (including C-terminal 6xHis + EPEA tag)

<400>  19

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Gly Tyr 
            20                  25                  30          


Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ser Cys Ile Ser Ser Ser Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Ala Trp Ser Pro Ser Thr Gly Cys Gly Ser Tyr Tyr Val Gln 
            100                 105                 110         


Arg Pro Pro Gly Tyr Asp Phe Trp Gly Gln Gly Thr Gln Val Thr Val 
        115                 120                 125             


Ser Ser His His His His His His Glu Pro Glu Ala 
    130                 135                 140 


<210>  20
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16239 GST-Nb (including C-terminal 6xHis + EPEA tag)

<400>  20

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Arg Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asn Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Ser Trp Asn Gly Gly Thr Thr Asn Ser Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Val Pro Tyr Leu Ser Gly Thr Arg Tyr Tyr Thr Gly 
            100                 105                 110         


Glu Val Asp Tyr Asp Thr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser His His His His His His Glu Pro Glu Ala 
    130                 135                 


<210>  21
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16240 GST-Nb_Y109A (including C-terminal 6xHis + EPEA tag)

<400>  21

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Arg Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asn Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Ser Trp Asn Gly Gly Thr Thr Asn Ser Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Arg Val Pro Tyr Leu Ser Gly Thr Arg Ala Tyr Thr Gly 
            100                 105                 110         


Glu Val Asp Tyr Asp Thr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser His His His His His His Glu Pro Glu Ala 
    130                 135                 


<210>  22
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glutathione S-transferase (GST)

<400>  22

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys 
    210                 215             


<210>  23
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA15839 SMT3-Nb (including C-terminal 6xHis + EPEA tag)

<400>  23

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Ala Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Thr Leu Ser Cys Thr Ala Ser Gly Ser Ile Val Ser Met Asn 
            20                  25                  30          


Ser Met Gly Trp Phe Arg Arg Ala Pro Trp Lys Gly Arg Glu Trp Leu 
        35                  40                  45              


Gly Asp Ile Gly Arg Asp Gly Arg Thr Ser Tyr Ala Ser Ser Val Val 
    50                  55                  60                  


Gly Arg Phe His Ile Ser Arg Asp Glu Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Thr Arg Leu Lys Pro Glu Asp Ala Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Val Pro Phe Gly Asp Asp Asp Val Gly Glu Trp Glu Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His Glu 
        115                 120                 125             


Pro Glu Ala 
    130     


<210>  24
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16687 SMT3-Nb_D50A (including C-terminal 6xHis + EPEA tag)

<400>  24

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Ala Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Thr Leu Ser Cys Thr Ala Ser Gly Ser Ile Val Ser Met Asn 
            20                  25                  30          


Ser Met Gly Trp Phe Arg Arg Ala Pro Trp Lys Gly Arg Glu Trp Leu 
        35                  40                  45              


Gly Ala Ile Gly Arg Asp Gly Arg Thr Ser Tyr Ala Ser Ser Val Val 
    50                  55                  60                  


Gly Arg Phe His Ile Ser Arg Asp Glu Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Thr Arg Leu Lys Pro Glu Asp Ala Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Val Pro Phe Gly Asp Asp Asp Val Gly Glu Trp Glu Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His Glu 
        115                 120                 125             


Pro Glu Ala 
    130     


<210>  25
<211>  114
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  25

Met His His His His His His Ser Ser Gly Ser Asp Ser Glu Val Asn 
1               5                   10                  15      


Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His 
            20                  25                  30          


Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile 
        35                  40                  45              


Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg 
    50                  55                  60                  


Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg 
65                  70                  75                  80  


Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp 
                85                  90                  95      


Ile Ile Glu Ala His Arg Glu Gln Ile Ser Ser Gly Leu Glu Val Leu 
            100                 105                 110         


Phe Gln 
        


<210>  26
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16964 mCherry Nb (including C-terminal 6xHis + EPEA tag)

<400>  26

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Asp Phe Ser Phe Asp 
            20                  25                  30          


Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Ser Gly Leu Arg Ile Gly Tyr Ser Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Thr Thr Val His 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Gly Ser His Ala Thr Thr Lys Ala Gln Leu Tyr Asp Tyr Glu 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125             


His His Glu Pro Glu Ala 
    130                 


<210>  27
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA17302 mCherry Nb (including C-terminal 6xHis + EPEA tag)

<400>  27

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Leu Pro Phe Ser Arg Tyr 
            20                  25                  30          


Asn Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Asp Ile Asp Trp Ser Gly Asp Ser Thr Tyr Asp Leu Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Leu Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala His Phe Lys Phe Ile Leu Ala Gln Arg Ile Lys Trp Gly Asp 
            100                 105                 110         


Met Asp Tyr Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  28
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA17341 mCherry Nb (CA17302 mutant I103A) (including C-terminal 
       6xHis + EPEA tag)

<400>  28

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Leu Pro Phe Ser Arg Tyr 
            20                  25                  30          


Asn Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Asp Ile Asp Trp Ser Gly Asp Ser Thr Tyr Asp Leu Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Leu Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala His Phe Lys Phe Ala Leu Ala Gln Arg Ile Lys Trp Gly Asp 
            100                 105                 110         


Met Asp Tyr Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser His His 
        115                 120                 125             


His His His His Glu Pro Glu Ala 
    130                 135     


<210>  29
<211>  415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry (FmlH_lectin_mCherry_his) (CA17337)

<400>  29

Phe Ser Cys Asn Val Asp Gly Gly Ser Ser Ile Gly Ala Gly Thr Thr 
1               5                   10                  15      


Ser Val Tyr Val Asn Leu Asp Pro Val Ile Gln Pro Gly Gln Asn Leu 
            20                  25                  30          


Val Val Asp Leu Ser Gln His Ile Ser Cys Trp Asn Asp Tyr Gly Gly 
        35                  40                  45              


Trp Tyr Asp Thr Asp His Ile Asn Leu Val Gln Gly Ser Ala Phe Ala 
    50                  55                  60                  


Gly Ser Leu Gln Ser Tyr Lys Gly Ser Leu Tyr Trp Asn Asn Val Thr 
65                  70                  75                  80  


Tyr Pro Phe Pro Leu Thr Thr Asn Thr Asn Val Leu Asp Ile Gly Asp 
                85                  90                  95      


Lys Thr Pro Met Pro Leu Pro Leu Lys Leu Tyr Ile Thr Pro Val Gly 
            100                 105                 110         


Ala Ala Gly Gly Val Val Ile Lys Ala Gly Glu Val Ile Ala Arg Ile 
        115                 120                 125             


His Met Tyr Lys Ile Ala Thr Leu Gly Ser Gly Asn Pro Arg Asn Phe 
    130                 135                 140                 


Thr Trp Asn Ile Ile Ser Asn Asn Ser Val Val Met Pro Thr Gly Leu 
145                 150                 155                 160 


Asn Ala Ser Leu Tyr Lys Arg Ala Gly Ser Gly Leu Asn Gly Val Ser 
                165                 170                 175     


Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe 
            180                 185                 190         


Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu 
        195                 200                 205             


Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu 
    210                 215                 220                 


Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser 
225                 230                 235                 240 


Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp 
                245                 250                 255     


Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu 
            260                 265                 270         


Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp 
        275                 280                 285             


Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly 
    290                 295                 300                 


Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly 
305                 310                 315                 320 


Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys 
                325                 330                 335     


Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp 
            340                 345                 350         


Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro 
        355                 360                 365             


Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu 
    370                 375                 380                 


Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser 
385                 390                 395                 400 


Thr Gly Gly Met Asp Glu Leu Tyr Lys His His His His His His 
                405                 410                 415 


<210>  30
<211>  901
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16383 Factor IX functionalized Nb stripper: Megabody 
       MbCA10309YgjK (including C-terminal 6xHis + EPEA tag)

<400>  30

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Glu Glu Thr 
1               5                   10                  15      


Gln Ser Gly Leu Asn Asn Tyr Ala Arg Val Val Glu Lys Gly Gln Tyr 
            20                  25                  30          


Asp Ser Leu Glu Ile Pro Ala Gln Val Ala Ala Ser Trp Glu Ser Gly 
        35                  40                  45              


Arg Asp Asp Ala Ala Val Phe Gly Phe Ile Asp Lys Glu Gln Leu Asp 
    50                  55                  60                  


Lys Tyr Val Ala Asn Gly Gly Lys Arg Ser Asp Trp Thr Val Lys Phe 
65                  70                  75                  80  


Ala Glu Asn Arg Ser Gln Asp Gly Thr Leu Leu Gly Tyr Ser Leu Leu 
                85                  90                  95      


Gln Glu Ser Val Asp Gln Ala Ser Tyr Met Tyr Ser Asp Asn His Tyr 
            100                 105                 110         


Leu Ala Glu Met Ala Thr Ile Leu Gly Lys Pro Glu Glu Ala Lys Arg 
        115                 120                 125             


Tyr Arg Gln Leu Ala Gln Gln Leu Ala Asp Tyr Ile Asn Thr Cys Met 
    130                 135                 140                 


Phe Asp Pro Thr Thr Gln Phe Tyr Tyr Asp Val Arg Ile Glu Asp Lys 
145                 150                 155                 160 


Pro Leu Ala Asn Gly Cys Ala Gly Lys Pro Ile Val Glu Arg Gly Lys 
                165                 170                 175     


Gly Pro Glu Gly Trp Ser Pro Leu Phe Asn Gly Ala Ala Thr Gln Ala 
            180                 185                 190         


Asn Ala Asp Ala Val Val Lys Val Met Leu Asp Pro Lys Glu Phe Asn 
        195                 200                 205             


Thr Phe Val Pro Leu Gly Thr Ala Ala Leu Thr Asn Pro Ala Phe Gly 
    210                 215                 220                 


Ala Asp Ile Tyr Trp Arg Gly Arg Val Trp Val Asp Gln Phe Trp Phe 
225                 230                 235                 240 


Gly Leu Lys Gly Met Glu Arg Tyr Gly Tyr Arg Asp Asp Ala Leu Lys 
                245                 250                 255     


Leu Ala Asp Thr Phe Phe Arg His Ala Lys Gly Leu Thr Ala Asp Gly 
            260                 265                 270         


Pro Ile Gln Glu Asn Tyr Asn Pro Leu Thr Gly Ala Gln Gln Gly Ala 
        275                 280                 285             


Pro Asn Phe Ser Trp Ser Ala Ala His Leu Tyr Met Leu Tyr Asn Asp 
    290                 295                 300                 


Phe Phe Arg Lys Gln Ala Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser 
305                 310                 315                 320 


Gly Gly Gly Gly Ser Gly Asn Ala Asp Asn Tyr Lys Asn Val Ile Asn 
                325                 330                 335     


Arg Thr Gly Ala Pro Gln Tyr Met Lys Asp Tyr Asp Tyr Asp Asp His 
            340                 345                 350         


Gln Arg Phe Asn Pro Phe Phe Asp Leu Gly Ala Trp His Gly His Leu 
        355                 360                 365             


Leu Pro Asp Gly Pro Asn Thr Met Gly Gly Phe Pro Gly Val Ala Leu 
    370                 375                 380                 


Leu Thr Glu Glu Tyr Ile Asn Phe Met Ala Ser Asn Phe Asp Arg Leu 
385                 390                 395                 400 


Thr Val Trp Gln Asp Gly Lys Lys Val Asp Phe Thr Leu Glu Ala Tyr 
                405                 410                 415     


Ser Ile Pro Gly Ala Leu Val Gln Lys Leu Thr Ala Lys Asp Val Gln 
            420                 425                 430         


Val Glu Met Thr Leu Arg Phe Ala Thr Pro Arg Thr Ser Leu Leu Glu 
        435                 440                 445             


Thr Lys Ile Thr Ser Asn Lys Pro Leu Asp Leu Val Trp Asp Gly Glu 
    450                 455                 460                 


Leu Leu Glu Lys Leu Glu Ala Lys Glu Gly Lys Pro Leu Ser Asp Lys 
465                 470                 475                 480 


Thr Ile Ala Gly Glu Tyr Pro Asp Tyr Gln Arg Lys Ile Ser Ala Thr 
                485                 490                 495     


Arg Asp Gly Leu Lys Val Thr Phe Gly Lys Val Arg Ala Thr Trp Asp 
            500                 505                 510         


Leu Leu Thr Ser Gly Glu Ser Glu Tyr Gln Val His Lys Ser Leu Pro 
        515                 520                 525             


Val Gln Thr Glu Ile Asn Gly Asn Arg Phe Thr Ser Lys Ala His Ile 
    530                 535                 540                 


Asn Gly Ser Thr Thr Leu Tyr Thr Thr Tyr Ser His Leu Leu Thr Ala 
545                 550                 555                 560 


Gln Glu Val Ser Lys Glu Gln Met Gln Ile Arg Asp Ile Leu Ala Arg 
                565                 570                 575     


Pro Ala Phe Tyr Leu Thr Ala Ser Gln Gln Arg Trp Glu Glu Tyr Leu 
            580                 585                 590         


Lys Lys Gly Leu Thr Asn Pro Asp Ala Thr Pro Glu Gln Thr Arg Val 
        595                 600                 605             


Ala Val Lys Ala Ile Glu Thr Leu Asn Gly Asn Trp Arg Ser Pro Gly 
    610                 615                 620                 


Gly Ala Val Lys Phe Asn Thr Val Thr Pro Ser Val Thr Gly Arg Trp 
625                 630                 635                 640 


Phe Ser Gly Asn Gln Thr Trp Pro Trp Asp Thr Trp Lys Gln Ala Phe 
                645                 650                 655     


Ala Met Ala His Phe Asn Pro Asp Ile Ala Lys Glu Asn Ile Arg Ala 
            660                 665                 670         


Val Phe Ser Trp Gln Ile Gln Pro Gly Asp Ser Val Arg Pro Gln Asp 
        675                 680                 685             


Val Gly Phe Val Pro Asp Leu Ile Ala Trp Asn Leu Ser Pro Glu Arg 
    690                 695                 700                 


Gly Gly Asp Gly Gly Asn Trp Asn Glu Arg Asn Thr Lys Pro Ser Leu 
705                 710                 715                 720 


Ala Ala Trp Ser Val Met Glu Val Tyr Asn Val Thr Gln Asp Lys Thr 
                725                 730                 735     


Trp Val Ala Glu Met Tyr Pro Lys Leu Val Ala Tyr His Asp Trp Trp 
            740                 745                 750         


Leu Arg Asn Arg Asp His Asn Gly Asn Gly Val Pro Glu Tyr Gly Ala 
        755                 760                 765             


Thr Arg Asp Lys Ala His Asn Thr Glu Ser Gly Glu Met Leu Phe Thr 
    770                 775                 780                 


Val Lys Lys Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ile Gly Phe 
785                 790                 795                 800 


Ser Ile Tyr Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg 
                805                 810                 815     


Glu Leu Val Ala Ser Ile Ala Gly Asn Glu Asp Lys Asp Tyr Val Glu 
            820                 825                 830         


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Tyr Ala Lys Asn Thr 
        835                 840                 845             


Leu Tyr Leu Glu Met Ser Gly Leu Lys Phe Glu Asp Thr Ala Met Tyr 
    850                 855                 860                 


Tyr Cys Ala Ala Gly Leu Arg Arg Gly Val Ser Leu Thr Pro Tyr Tyr 
865                 870                 875                 880 


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
                885                 890                 895     


His Glu Pro Glu Ala 
            900     


<210>  31
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA11143 FIX Nb (including C-terminal 6xHis + EPEA tag)

<400>  31

Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asn Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Asn Trp Asn Gly Arg Asn Thr Tyr Tyr Ala Glu Ser Val 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Glu Ile Ile Thr Ser Gly Gly Pro Thr Gly Lys Tyr Asp Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
        115                 120                 125             


His Glu Pro Glu Ala 
    130             


<210>  32
<211>  760
<212>  PRT
<213>  Escherichia coli

<400>  32

Asn Ala Asp Asn Tyr Lys Asn Val Ile Asn Arg Thr Gly Ala Pro Gln 
1               5                   10                  15      


Tyr Met Lys Asp Tyr Asp Tyr Asp Asp His Gln Arg Phe Asn Pro Phe 
            20                  25                  30          


Phe Asp Leu Gly Ala Trp His Gly His Leu Leu Pro Asp Gly Pro Asn 
        35                  40                  45              


Thr Met Gly Gly Phe Pro Gly Val Ala Leu Leu Thr Glu Glu Tyr Ile 
    50                  55                  60                  


Asn Phe Met Ala Ser Asn Phe Asp Arg Leu Thr Val Trp Gln Asp Gly 
65                  70                  75                  80  


Lys Lys Val Asp Phe Thr Leu Glu Ala Tyr Ser Ile Pro Gly Ala Leu 
                85                  90                  95      


Val Gln Lys Leu Thr Ala Lys Asp Val Gln Val Glu Met Thr Leu Arg 
            100                 105                 110         


Phe Ala Thr Pro Arg Thr Ser Leu Leu Glu Thr Lys Ile Thr Ser Asn 
        115                 120                 125             


Lys Pro Leu Asp Leu Val Trp Asp Gly Glu Leu Leu Glu Lys Leu Glu 
    130                 135                 140                 


Ala Lys Glu Gly Lys Pro Leu Ser Asp Lys Thr Ile Ala Gly Glu Tyr 
145                 150                 155                 160 


Pro Asp Tyr Gln Arg Lys Ile Ser Ala Thr Arg Asp Gly Leu Lys Val 
                165                 170                 175     


Thr Phe Gly Lys Val Arg Ala Thr Trp Asp Leu Leu Thr Ser Gly Glu 
            180                 185                 190         


Ser Glu Tyr Gln Val His Lys Ser Leu Pro Val Gln Thr Glu Ile Asn 
        195                 200                 205             


Gly Asn Arg Phe Thr Ser Lys Ala His Ile Asn Gly Ser Thr Thr Leu 
    210                 215                 220                 


Tyr Thr Thr Tyr Ser His Leu Leu Thr Ala Gln Glu Val Ser Lys Glu 
225                 230                 235                 240 


Gln Met Gln Ile Arg Asp Ile Leu Ala Arg Pro Ala Phe Tyr Leu Thr 
                245                 250                 255     


Ala Ser Gln Gln Arg Trp Glu Glu Tyr Leu Lys Lys Gly Leu Thr Asn 
            260                 265                 270         


Pro Asp Ala Thr Pro Glu Gln Thr Arg Val Ala Val Lys Ala Ile Glu 
        275                 280                 285             


Thr Leu Asn Gly Asn Trp Arg Ser Pro Gly Gly Ala Val Lys Phe Asn 
    290                 295                 300                 


Thr Val Thr Pro Ser Val Thr Gly Arg Trp Phe Ser Gly Asn Gln Thr 
305                 310                 315                 320 


Trp Pro Trp Asp Thr Trp Lys Gln Ala Phe Ala Met Ala His Phe Asn 
                325                 330                 335     


Pro Asp Ile Ala Lys Glu Asn Ile Arg Ala Val Phe Ser Trp Gln Ile 
            340                 345                 350         


Gln Pro Gly Asp Ser Val Arg Pro Gln Asp Val Gly Phe Val Pro Asp 
        355                 360                 365             


Leu Ile Ala Trp Asn Leu Ser Pro Glu Arg Gly Gly Asp Gly Gly Asn 
    370                 375                 380                 


Trp Asn Glu Arg Asn Thr Lys Pro Ser Leu Ala Ala Trp Ser Val Met 
385                 390                 395                 400 


Glu Val Tyr Asn Val Thr Gln Asp Lys Thr Trp Val Ala Glu Met Tyr 
                405                 410                 415     


Pro Lys Leu Val Ala Tyr His Asp Trp Trp Leu Arg Asn Arg Asp His 
            420                 425                 430         


Asn Gly Asn Gly Val Pro Glu Tyr Gly Ala Thr Arg Asp Lys Ala His 
        435                 440                 445             


Asn Thr Glu Ser Gly Glu Met Leu Phe Thr Val Lys Lys Gly Asp Lys 
    450                 455                 460                 


Glu Glu Thr Gln Ser Gly Leu Asn Asn Tyr Ala Arg Val Val Glu Lys 
465                 470                 475                 480 


Gly Gln Tyr Asp Ser Leu Glu Ile Pro Ala Gln Val Ala Ala Ser Trp 
                485                 490                 495     


Glu Ser Gly Arg Asp Asp Ala Ala Val Phe Gly Phe Ile Asp Lys Glu 
            500                 505                 510         


Gln Leu Asp Lys Tyr Val Ala Asn Gly Gly Lys Arg Ser Asp Trp Thr 
        515                 520                 525             


Val Lys Phe Ala Glu Asn Arg Ser Gln Asp Gly Thr Leu Leu Gly Tyr 
    530                 535                 540                 


Ser Leu Leu Gln Glu Ser Val Asp Gln Ala Ser Tyr Met Tyr Ser Asp 
545                 550                 555                 560 


Asn His Tyr Leu Ala Glu Met Ala Thr Ile Leu Gly Lys Pro Glu Glu 
                565                 570                 575     


Ala Lys Arg Tyr Arg Gln Leu Ala Gln Gln Leu Ala Asp Tyr Ile Asn 
            580                 585                 590         


Thr Cys Met Phe Asp Pro Thr Thr Gln Phe Tyr Tyr Asp Val Arg Ile 
        595                 600                 605             


Glu Asp Lys Pro Leu Ala Asn Gly Cys Ala Gly Lys Pro Ile Val Glu 
    610                 615                 620                 


Arg Gly Lys Gly Pro Glu Gly Trp Ser Pro Leu Phe Asn Gly Ala Ala 
625                 630                 635                 640 


Thr Gln Ala Asn Ala Asp Ala Val Val Lys Val Met Leu Asp Pro Lys 
                645                 650                 655     


Glu Phe Asn Thr Phe Val Pro Leu Gly Thr Ala Ala Leu Thr Asn Pro 
            660                 665                 670         


Ala Phe Gly Ala Asp Ile Tyr Trp Arg Gly Arg Val Trp Val Asp Gln 
        675                 680                 685             


Phe Trp Phe Gly Leu Lys Gly Met Glu Arg Tyr Gly Tyr Arg Asp Asp 
    690                 695                 700                 


Ala Leu Lys Leu Ala Asp Thr Phe Phe Arg His Ala Lys Gly Leu Thr 
705                 710                 715                 720 


Ala Asp Gly Pro Ile Gln Glu Asn Tyr Asn Pro Leu Thr Gly Ala Gln 
                725                 730                 735     


Gln Gly Ala Pro Asn Phe Ser Trp Ser Ala Ala His Leu Tyr Met Leu 
            740                 745                 750         


Tyr Asn Asp Phe Phe Arg Lys Gln 
        755                 760 


<210>  33
<211>  904
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16388 FIX functionalized Nb trapper: Megabody MbCA11143YgjK 
       (including C-terminal 6xHis + EPEA tag)

<400>  33

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Glu Glu Thr 
1               5                   10                  15      


Gln Ser Gly Leu Asn Asn Tyr Ala Arg Val Val Glu Lys Gly Gln Tyr 
            20                  25                  30          


Asp Ser Leu Glu Ile Pro Ala Gln Val Ala Ala Ser Trp Glu Ser Gly 
        35                  40                  45              


Arg Asp Asp Ala Ala Val Phe Gly Phe Ile Asp Lys Glu Gln Leu Asp 
    50                  55                  60                  


Lys Tyr Val Ala Asn Gly Gly Lys Arg Ser Asp Trp Thr Val Lys Phe 
65                  70                  75                  80  


Ala Glu Asn Arg Ser Gln Asp Gly Thr Leu Leu Gly Tyr Ser Leu Leu 
                85                  90                  95      


Gln Glu Ser Val Asp Gln Ala Ser Tyr Met Tyr Ser Asp Asn His Tyr 
            100                 105                 110         


Leu Ala Glu Met Ala Thr Ile Leu Gly Lys Pro Glu Glu Ala Lys Arg 
        115                 120                 125             


Tyr Arg Gln Leu Ala Gln Gln Leu Ala Asp Tyr Ile Asn Thr Cys Met 
    130                 135                 140                 


Phe Asp Pro Thr Thr Gln Phe Tyr Tyr Asp Val Arg Ile Glu Asp Lys 
145                 150                 155                 160 


Pro Leu Ala Asn Gly Cys Ala Gly Lys Pro Ile Val Glu Arg Gly Lys 
                165                 170                 175     


Gly Pro Glu Gly Trp Ser Pro Leu Phe Asn Gly Ala Ala Thr Gln Ala 
            180                 185                 190         


Asn Ala Asp Ala Val Val Lys Val Met Leu Asp Pro Lys Glu Phe Asn 
        195                 200                 205             


Thr Phe Val Pro Leu Gly Thr Ala Ala Leu Thr Asn Pro Ala Phe Gly 
    210                 215                 220                 


Ala Asp Ile Tyr Trp Arg Gly Arg Val Trp Val Asp Gln Phe Trp Phe 
225                 230                 235                 240 


Gly Leu Lys Gly Met Glu Arg Tyr Gly Tyr Arg Asp Asp Ala Leu Lys 
                245                 250                 255     


Leu Ala Asp Thr Phe Phe Arg His Ala Lys Gly Leu Thr Ala Asp Gly 
            260                 265                 270         


Pro Ile Gln Glu Asn Tyr Asn Pro Leu Thr Gly Ala Gln Gln Gly Ala 
        275                 280                 285             


Pro Asn Phe Ser Trp Ser Ala Ala His Leu Tyr Met Leu Tyr Asn Asp 
    290                 295                 300                 


Phe Phe Arg Lys Gln Ala Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser 
305                 310                 315                 320 


Gly Gly Gly Gly Ser Gly Asn Ala Asp Asn Tyr Lys Asn Val Ile Asn 
                325                 330                 335     


Arg Thr Gly Ala Pro Gln Tyr Met Lys Asp Tyr Asp Tyr Asp Asp His 
            340                 345                 350         


Gln Arg Phe Asn Pro Phe Phe Asp Leu Gly Ala Trp His Gly His Leu 
        355                 360                 365             


Leu Pro Asp Gly Pro Asn Thr Met Gly Gly Phe Pro Gly Val Ala Leu 
    370                 375                 380                 


Leu Thr Glu Glu Tyr Ile Asn Phe Met Ala Ser Asn Phe Asp Arg Leu 
385                 390                 395                 400 


Thr Val Trp Gln Asp Gly Lys Lys Val Asp Phe Thr Leu Glu Ala Tyr 
                405                 410                 415     


Ser Ile Pro Gly Ala Leu Val Gln Lys Leu Thr Ala Lys Asp Val Gln 
            420                 425                 430         


Val Glu Met Thr Leu Arg Phe Ala Thr Pro Arg Thr Ser Leu Leu Glu 
        435                 440                 445             


Thr Lys Ile Thr Ser Asn Lys Pro Leu Asp Leu Val Trp Asp Gly Glu 
    450                 455                 460                 


Leu Leu Glu Lys Leu Glu Ala Lys Glu Gly Lys Pro Leu Ser Asp Lys 
465                 470                 475                 480 


Thr Ile Ala Gly Glu Tyr Pro Asp Tyr Gln Arg Lys Ile Ser Ala Thr 
                485                 490                 495     


Arg Asp Gly Leu Lys Val Thr Phe Gly Lys Val Arg Ala Thr Trp Asp 
            500                 505                 510         


Leu Leu Thr Ser Gly Glu Ser Glu Tyr Gln Val His Lys Ser Leu Pro 
        515                 520                 525             


Val Gln Thr Glu Ile Asn Gly Asn Arg Phe Thr Ser Lys Ala His Ile 
    530                 535                 540                 


Asn Gly Ser Thr Thr Leu Tyr Thr Thr Tyr Ser His Leu Leu Thr Ala 
545                 550                 555                 560 


Gln Glu Val Ser Lys Glu Gln Met Gln Ile Arg Asp Ile Leu Ala Arg 
                565                 570                 575     


Pro Ala Phe Tyr Leu Thr Ala Ser Gln Gln Arg Trp Glu Glu Tyr Leu 
            580                 585                 590         


Lys Lys Gly Leu Thr Asn Pro Asp Ala Thr Pro Glu Gln Thr Arg Val 
        595                 600                 605             


Ala Val Lys Ala Ile Glu Thr Leu Asn Gly Asn Trp Arg Ser Pro Gly 
    610                 615                 620                 


Gly Ala Val Lys Phe Asn Thr Val Thr Pro Ser Val Thr Gly Arg Trp 
625                 630                 635                 640 


Phe Ser Gly Asn Gln Thr Trp Pro Trp Asp Thr Trp Lys Gln Ala Phe 
                645                 650                 655     


Ala Met Ala His Phe Asn Pro Asp Ile Ala Lys Glu Asn Ile Arg Ala 
            660                 665                 670         


Val Phe Ser Trp Gln Ile Gln Pro Gly Asp Ser Val Arg Pro Gln Asp 
        675                 680                 685             


Val Gly Phe Val Pro Asp Leu Ile Ala Trp Asn Leu Ser Pro Glu Arg 
    690                 695                 700                 


Gly Gly Asp Gly Gly Asn Trp Asn Glu Arg Asn Thr Lys Pro Ser Leu 
705                 710                 715                 720 


Ala Ala Trp Ser Val Met Glu Val Tyr Asn Val Thr Gln Asp Lys Thr 
                725                 730                 735     


Trp Val Ala Glu Met Tyr Pro Lys Leu Val Ala Tyr His Asp Trp Trp 
            740                 745                 750         


Leu Arg Asn Arg Asp His Asn Gly Asn Gly Val Pro Glu Tyr Gly Ala 
        755                 760                 765             


Thr Arg Asp Lys Ala His Asn Thr Glu Ser Gly Glu Met Leu Phe Thr 
    770                 775                 780                 


Val Lys Lys Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
785                 790                 795                 800 


Asp Asn Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                805                 810                 815     


Glu Trp Val Ser Gly Ile Asn Trp Asn Gly Arg Asn Thr Tyr Tyr Ala 
            820                 825                 830         


Glu Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
        835                 840                 845             


Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Val 
    850                 855                 860                 


Tyr Tyr Cys Ala Lys Glu Ile Ile Thr Ser Gly Gly Pro Thr Gly Lys 
865                 870                 875                 880 


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
                885                 890                 895     


His His His His Glu Pro Glu Ala 
            900                 


<210>  34
<211>  1029
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16607 eGFP-tagged human recombinant glucocorticoid receptor 
       P04150 (eGFP-6His-TEV-GR)

<400>  34

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys His 
225                 230                 235                 240 


His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Asp Ser Lys 
                245                 250                 255     


Glu Ser Leu Thr Pro Gly Arg Glu Glu Asn Pro Ser Ser Val Leu Ala 
            260                 265                 270         


Gln Glu Arg Gly Asp Val Met Asp Phe Tyr Lys Thr Leu Arg Gly Gly 
        275                 280                 285             


Ala Thr Val Lys Val Ser Ala Ser Ser Pro Ser Leu Ala Val Ala Ser 
    290                 295                 300                 


Gln Ser Asp Ser Lys Gln Arg Arg Leu Leu Val Asp Phe Pro Lys Gly 
305                 310                 315                 320 


Ser Val Ser Asn Ala Gln Gln Pro Asp Leu Ser Lys Ala Val Ser Leu 
                325                 330                 335     


Ser Met Gly Leu Tyr Met Gly Glu Thr Glu Thr Lys Val Met Gly Asn 
            340                 345                 350         


Asp Leu Gly Phe Pro Gln Gln Gly Gln Ile Ser Leu Ser Ser Gly Glu 
        355                 360                 365             


Thr Asp Leu Lys Leu Leu Glu Glu Ser Ile Ala Asn Leu Asn Arg Ser 
    370                 375                 380                 


Thr Ser Val Pro Glu Asn Pro Lys Ser Ser Ala Ser Thr Ala Val Ser 
385                 390                 395                 400 


Ala Ala Pro Thr Glu Lys Glu Phe Pro Lys Thr His Ser Asp Val Ser 
                405                 410                 415     


Ser Glu Gln Gln His Leu Lys Gly Gln Thr Gly Thr Asn Gly Gly Asn 
            420                 425                 430         


Val Lys Leu Tyr Thr Thr Asp Gln Ser Thr Phe Asp Ile Leu Gln Asp 
        435                 440                 445             


Leu Glu Phe Ser Ser Gly Ser Pro Gly Lys Glu Thr Asn Glu Ser Pro 
    450                 455                 460                 


Trp Arg Ser Asp Leu Leu Ile Asp Glu Asn Cys Leu Leu Ser Pro Leu 
465                 470                 475                 480 


Ala Gly Glu Asp Asp Ser Phe Leu Leu Glu Gly Asn Ser Asn Glu Asp 
                485                 490                 495     


Cys Lys Pro Leu Ile Leu Pro Asp Thr Lys Pro Lys Ile Lys Asp Asn 
            500                 505                 510         


Gly Asp Leu Val Leu Ser Ser Pro Ser Asn Val Thr Leu Pro Gln Val 
        515                 520                 525             


Lys Thr Glu Lys Glu Asp Phe Ile Glu Leu Cys Thr Pro Gly Val Ile 
    530                 535                 540                 


Lys Gln Glu Lys Leu Gly Thr Val Tyr Cys Gln Ala Ser Phe Pro Gly 
545                 550                 555                 560 


Ala Asn Ile Ile Gly Asn Lys Met Ser Ala Ile Ser Val His Gly Val 
                565                 570                 575     


Ser Thr Ser Gly Gly Gln Met Tyr His Tyr Asp Met Asn Thr Ala Ser 
            580                 585                 590         


Leu Ser Gln Gln Gln Asp Gln Lys Pro Ile Phe Asn Val Ile Pro Pro 
        595                 600                 605             


Ile Pro Val Gly Ser Glu Asn Trp Asn Arg Cys Gln Gly Ser Gly Asp 
    610                 615                 620                 


Asp Asn Leu Thr Ser Leu Gly Thr Leu Asn Phe Pro Gly Arg Thr Val 
625                 630                 635                 640 


Phe Ser Asn Gly Tyr Ser Ser Pro Ser Met Arg Pro Asp Val Ser Ser 
                645                 650                 655     


Pro Pro Ser Ser Ser Ser Thr Ala Thr Thr Gly Pro Pro Pro Lys Leu 
            660                 665                 670         


Cys Leu Val Cys Ser Asp Glu Ala Ser Gly Cys His Tyr Gly Val Leu 
        675                 680                 685             


Thr Cys Gly Ser Cys Lys Val Phe Phe Lys Arg Ala Val Glu Gly Gln 
    690                 695                 700                 


His Asn Tyr Leu Cys Ala Gly Arg Asn Asp Cys Ile Ile Asp Lys Ile 
705                 710                 715                 720 


Arg Arg Lys Asn Cys Pro Ala Cys Arg Tyr Arg Lys Cys Leu Gln Ala 
                725                 730                 735     


Gly Met Asn Leu Glu Ala Arg Lys Thr Lys Lys Lys Ile Lys Gly Ile 
            740                 745                 750         


Gln Gln Ala Thr Thr Gly Val Ser Gln Glu Thr Ser Glu Asn Pro Gly 
        755                 760                 765             


Asn Lys Thr Ile Val Pro Ala Thr Leu Pro Gln Leu Thr Pro Thr Leu 
    770                 775                 780                 


Val Ser Leu Leu Glu Val Ile Glu Pro Glu Val Leu Tyr Ala Gly Tyr 
785                 790                 795                 800 


Asp Ser Ser Val Pro Asp Ser Thr Trp Arg Ile Met Thr Thr Leu Asn 
                805                 810                 815     


Met Leu Gly Gly Arg Gln Val Ile Ala Ala Val Lys Trp Ala Lys Ala 
            820                 825                 830         


Ile Pro Gly Phe Arg Asn Leu His Leu Asp Asp Gln Met Thr Leu Leu 
        835                 840                 845             


Gln Tyr Ser Trp Met Phe Leu Met Ala Phe Ala Leu Gly Trp Arg Ser 
    850                 855                 860                 


Tyr Arg Gln Ser Ser Ala Asn Leu Leu Cys Phe Ala Pro Asp Leu Ile 
865                 870                 875                 880 


Ile Asn Glu Gln Arg Met Thr Leu Pro Cys Met Tyr Asp Gln Cys Lys 
                885                 890                 895     


His Met Leu Tyr Val Ser Ser Glu Leu His Arg Leu Gln Val Ser Tyr 
            900                 905                 910         


Glu Glu Tyr Leu Cys Met Lys Thr Leu Leu Leu Leu Ser Ser Val Pro 
        915                 920                 925             


Lys Asp Gly Leu Lys Ser Gln Glu Leu Phe Asp Glu Ile Arg Met Thr 
    930                 935                 940                 


Tyr Ile Lys Glu Leu Gly Lys Ala Ile Val Lys Arg Glu Gly Asn Ser 
945                 950                 955                 960 


Ser Gln Asn Trp Gln Arg Phe Tyr Gln Leu Thr Lys Leu Leu Asp Ser 
                965                 970                 975     


Met His Glu Val Val Glu Asn Leu Leu Asn Tyr Cys Phe Gln Thr Phe 
            980                 985                 990         


Leu Asp Lys Thr Met Ser Ile Glu  Phe Pro Glu Met Leu  Ala Glu Ile 
        995                 1000                 1005             


Ile Thr  Asn Gln Ile Pro Lys  Tyr Ser Asn Gly Asn  Ile Lys Lys 
    1010                 1015                 1020             


Leu Leu  Phe His Gln Lys 
    1025                 


<210>  35
<211>  1172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA16976 eGFP-tagged human recombinant androgen receptor P10275 
       (eGFP-6His-TEV-ARb)

<400>  35

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys His 
225                 230                 235                 240 


His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Glu Val Gln 
                245                 250                 255     


Leu Gly Leu Gly Arg Val Tyr Pro Arg Pro Pro Ser Lys Thr Tyr Arg 
            260                 265                 270         


Gly Ala Phe Gln Asn Leu Phe Gln Ser Val Arg Glu Val Ile Gln Asn 
        275                 280                 285             


Pro Gly Pro Arg His Pro Glu Ala Ala Ser Ala Ala Pro Pro Gly Ala 
    290                 295                 300                 


Ser Leu Leu Leu Leu Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln 
305                 310                 315                 320 


Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Glu Thr Ser Pro 
                325                 330                 335     


Arg Gln Gln Gln Gln Gln Gln Gly Glu Asp Gly Ser Pro Gln Ala His 
            340                 345                 350         


Arg Arg Gly Pro Thr Gly Tyr Leu Val Leu Asp Glu Glu Gln Gln Pro 
        355                 360                 365             


Ser Gln Pro Gln Ser Ala Leu Glu Cys His Pro Glu Arg Gly Cys Val 
    370                 375                 380                 


Pro Glu Pro Gly Ala Ala Val Ala Ala Ser Lys Gly Leu Pro Gln Gln 
385                 390                 395                 400 


Leu Pro Ala Pro Pro Asp Glu Asp Asp Ser Ala Ala Pro Ser Thr Leu 
                405                 410                 415     


Ser Leu Leu Gly Pro Thr Phe Pro Gly Leu Ser Ser Cys Ser Ala Asp 
            420                 425                 430         


Leu Lys Asp Ile Leu Ser Glu Ala Ser Thr Met Gln Leu Leu Gln Gln 
        435                 440                 445             


Gln Gln Gln Glu Ala Val Ser Glu Gly Ser Ser Ser Gly Arg Ala Arg 
    450                 455                 460                 


Glu Ala Ser Gly Ala Pro Thr Ser Ser Lys Asp Asn Tyr Leu Gly Gly 
465                 470                 475                 480 


Thr Ser Thr Ile Ser Asp Asn Ala Lys Glu Leu Cys Lys Ala Val Ser 
                485                 490                 495     


Val Ser Met Gly Leu Gly Val Glu Ala Leu Glu His Leu Ser Pro Gly 
            500                 505                 510         


Glu Gln Leu Arg Gly Asp Cys Met Tyr Ala Pro Leu Leu Gly Val Pro 
        515                 520                 525             


Pro Ala Val Arg Pro Thr Pro Cys Ala Pro Leu Ala Glu Cys Lys Gly 
    530                 535                 540                 


Ser Leu Leu Asp Asp Ser Ala Gly Lys Ser Thr Glu Asp Thr Ala Glu 
545                 550                 555                 560 


Tyr Ser Pro Phe Lys Gly Gly Tyr Thr Lys Gly Leu Glu Gly Glu Ser 
                565                 570                 575     


Leu Gly Cys Ser Gly Ser Ala Ala Ala Gly Ser Ser Gly Thr Leu Glu 
            580                 585                 590         


Leu Pro Ser Thr Leu Ser Leu Tyr Lys Ser Gly Ala Leu Asp Glu Ala 
        595                 600                 605             


Ala Ala Tyr Gln Ser Arg Asp Tyr Tyr Asn Phe Pro Leu Ala Leu Ala 
    610                 615                 620                 


Gly Pro Pro Pro Pro Pro Pro Pro Pro His Pro His Ala Arg Ile Lys 
625                 630                 635                 640 


Leu Glu Asn Pro Leu Asp Tyr Gly Ser Ala Trp Ala Ala Ala Ala Ala 
                645                 650                 655     


Gln Cys Arg Tyr Gly Asp Leu Ala Ser Leu His Gly Ala Gly Ala Ala 
            660                 665                 670         


Gly Pro Gly Ser Gly Ser Pro Ser Ala Ala Ala Ser Ser Ser Trp His 
        675                 680                 685             


Thr Leu Phe Thr Ala Glu Glu Gly Gln Leu Tyr Gly Pro Cys Gly Gly 
    690                 695                 700                 


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
705                 710                 715                 720 


Gly Gly Gly Gly Gly Glu Ala Gly Ala Val Ala Pro Tyr Gly Tyr Thr 
                725                 730                 735     


Arg Pro Pro Gln Gly Leu Ala Gly Gln Glu Ser Asp Phe Thr Ala Pro 
            740                 745                 750         


Asp Val Trp Tyr Pro Gly Gly Met Val Ser Arg Val Pro Tyr Pro Ser 
        755                 760                 765             


Pro Thr Cys Val Lys Ser Glu Met Gly Pro Trp Met Asp Ser Tyr Ser 
    770                 775                 780                 


Gly Pro Tyr Gly Asp Met Arg Leu Glu Thr Ala Arg Asp His Val Leu 
785                 790                 795                 800 


Pro Ile Asp Tyr Tyr Phe Pro Pro Gln Lys Thr Cys Leu Ile Cys Gly 
                805                 810                 815     


Asp Glu Ala Ser Gly Cys His Tyr Gly Ala Leu Thr Cys Gly Ser Cys 
            820                 825                 830         


Lys Val Phe Phe Lys Arg Ala Ala Glu Gly Lys Gln Lys Tyr Leu Cys 
        835                 840                 845             


Ala Ser Arg Asn Asp Cys Thr Ile Asp Lys Phe Arg Arg Lys Asn Cys 
    850                 855                 860                 


Pro Ser Cys Arg Leu Arg Lys Cys Tyr Glu Ala Gly Met Thr Leu Gly 
865                 870                 875                 880 


Ala Arg Lys Leu Lys Lys Leu Gly Asn Leu Lys Leu Gln Glu Glu Gly 
                885                 890                 895     


Glu Ala Ser Ser Thr Thr Ser Pro Thr Glu Glu Thr Thr Gln Lys Leu 
            900                 905                 910         


Thr Val Ser His Ile Glu Gly Tyr Glu Cys Gln Pro Ile Phe Leu Asn 
        915                 920                 925             


Val Leu Glu Ala Ile Glu Pro Gly Val Val Cys Ala Gly His Asp Asn 
    930                 935                 940                 


Asn Gln Pro Asp Ser Phe Ala Ala Leu Leu Ser Ser Leu Asn Glu Leu 
945                 950                 955                 960 


Gly Glu Arg Gln Leu Val His Val Val Lys Trp Ala Lys Ala Leu Pro 
                965                 970                 975     


Gly Phe Arg Asn Leu His Val Asp Asp Gln Met Ala Val Ile Gln Tyr 
            980                 985                 990         


Ser Trp Met Gly Leu Met Val Phe  Ala Met Gly Trp Arg  Ser Phe Thr 
        995                 1000                 1005             


Asn Val  Asn Ser Arg Met Leu  Tyr Phe Ala Pro Asp  Leu Val Phe 
    1010                 1015                 1020             


Asn Glu  Tyr Arg Met His Lys  Ser Arg Met Tyr Ser  Gln Cys Val 
    1025                 1030                 1035             


Arg Met  Arg His Leu Ser Gln  Glu Phe Gly Trp Leu  Gln Ile Thr 
    1040                 1045                 1050             


Pro Gln  Glu Phe Leu Cys Met  Lys Ala Leu Leu Leu  Phe Ser Ile 
    1055                 1060                 1065             


Ile Pro  Val Asp Gly Leu Lys  Asn Gln Lys Phe Phe  Asp Glu Leu 
    1070                 1075                 1080             


Arg Met  Asn Tyr Ile Lys Glu  Leu Asp Arg Ile Ile  Ala Cys Lys 
    1085                 1090                 1095             


Arg Lys  Asn Pro Thr Ser Cys  Ser Arg Arg Phe Tyr  Gln Leu Thr 
    1100                 1105                 1110             


Lys Leu  Leu Asp Ser Val Gln  Pro Ile Ala Arg Glu  Leu His Gln 
    1115                 1120                 1125             


Phe Thr  Phe Asp Leu Leu Ile  Lys Ser His Met Val  Ser Val Asp 
    1130                 1135                 1140             


Phe Pro  Glu Met Met Ala Glu  Ile Ile Ser Val Gln  Val Pro Lys 
    1145                 1150                 1155             


Ile Leu  Ser Gly Lys Val Lys  Pro Ile Tyr Phe His  Thr Gln 
    1160                 1165                 1170         


