                         SEQUENCE LISTING

<110>  Intervet International BV
 
<120>  Multivalent HVT vector vaccine

<130>  24912

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  5498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Combined single expression cassette


<220>
<221>  promoter
<222>  (1)..(1391)
<223>  murine cytomegalovirus immediate early 1 gene promoter-enhancer

<220>
<221>  gene
<222>  (1423)..(2781)
<223>  IBDV strain F 52/70, VP2 gene

<220>
<221>  terminator
<222>  (2812)..(3021)
<223>  SV40 polyA signal + terminator

<220>
<221>  promoter
<222>  (3160)..(3520)
<223>  Human cytomegalovirus immediate early 1 gene core promoter

<220>
<221>  gene
<222>  (3545)..(5206)
<223>  NDV Clone 30 F-gene

<220>
<221>  terminator
<222>  (5218)..(5498)
<223>  hCMV IE1 gene terminator

<400>  1
aactccgccc gttttatgac tagaaccaat agtttttaat gccaaatgca ctgaaatccc       60

ctaatttgca aagccaaacg ccccctatgt gagtaatacg gggacttttt acccaatttc      120

ccaagcggaa agccccctaa tacactcata tggcatatga atcagcacgg tcatgcactc      180

taatggcggc ccatagggac tttccacata gggggcgttc accatttccc agcatagggg      240

tggtgactca atggccttta cccaagtaca ttgggtcaat gggaggtaag ccaatgggtt      300

tttcccatta ctggcaagca cactgagtca aatgggactt tccactgggt tttgcccaag      360

tacattgggt caatgggagg tgagccaatg ggaaaaaccc attgctgcca agtacactga      420

ctcaataggg actttccaat gggtttttcc attgttggca agcatataag gtcaatgtgg      480

gtgagtcaat agggactttc cattgtattc tgcccagtac ataaggtcaa tagggggtga      540

atcaacagga aagtcccatt ggagccaagt acactgcgtc aatagggact ttccattggg      600

ttttgcccag tacataaggt caatagggga tgagtcaatg ggaaaaaccc attggagcca      660

agtacactga ctcaataggg actttccatt gggttttgcc cagtacataa ggtcaatagg      720

gggtgagtca acaggaaagt cccattggag ccaagtacat tgagtcaata gggactttcc      780

aatgggtttt gcccagtaca taaggtcaat gggaggtaag ccaatgggtt tttcccatta      840

ctggcacgta tactgagtca ttagggactt tccaatgggt tttgcccagt acataaggtc      900

aataggggtg aatcaacagg aaagtcccat tggagccaag tacactgagt caatagggac      960

tttccattgg gttttgccca gtacaaaagg tcaatagggg gtgagtcaat gggtttttcc     1020

cattattggc acgtacataa ggtcaatagg ggtgagtcat tgggtttttc cagccaattt     1080

aattaaaacg ccatgtactt tcccaccatt gacgtcaatg ggctattgaa actaatgcaa     1140

cgtgaccttt aaacggtact ttcccatagc tgattaatgg gaaagtaccg ttctcgagcc     1200

aatacacgtc aatgggaagt gaaagggcag ccaaaacgta acaccgcccc ggttttcccc     1260

tggaaattcc atattggcac gcattctatt ggctgagctg cgttctacgt gggtataaga     1320

ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga ccaccgtaga acgcagagct     1380

cctcgctgca ggcggccgct ctagaactcg tcgatcgcag cgatgacaaa cctgcaagat     1440

caaacccaac agattgttcc gttcatacgg agccttctga tgccaacaac cggaccggcg     1500

tccattccgg acgacaccct ggagaagcac actctcaggt cagagacctc gacctacaat     1560

ttgactgtgg gggacacagg gtcagggcta attgtctttt tccctggatt ccctggctca     1620

attgtgggtg ctcactacac actgcagagc aatgggaact acaagttcga tcagatgctc     1680

ctgactgccc agaacctacc ggccagctac aactactgca gactagtgag tcggagtctc     1740

acagtgaggt caagcacact ccctggtggc gtttatgcac taaacggcac cataaacgcc     1800

gtgaccttcc aaggaagcct gagtgaactg acagatgtta gctacaatgg gttgatgtct     1860

gcaacagcca acatcaacga caaaattggg aatgtcctgg taggggaagg ggtcactgtc     1920

ctcagcctac ccacatcata tgatcttggg tatgtgaggc ttggtgaccc cattcccgct     1980

atagggcttg acccaaaaat ggtagctaca tgcgacagca gtgacaggcc cagagtctac     2040

accataactg cagccgatga ttaccaattc tcatcacagt accaaccagg tggggtaaca     2100

atcacactgt tctcagccaa cattgatgct atcacaagcc tcagcattgg gggagagctc     2160

gtgtttcaaa caagcgtcca aggccttgta ctgggcgcca ccatctacct tataggcttt     2220

gatgggactg cggtaatcac cagagctgta gccgcagata atgggctgac ggccggcacc     2280

gacaatctta tgccattcaa tcttgtcatt ccaaccaatg agataaccca gccaatcaca     2340

tccatcaaac tggagatagt gacctccaaa agtggtggtc aggcagggga tcagatgtca     2400

tggtcggcaa gtgggagcct agcagtgacg atccatggtg gcaactatcc aggggccctc     2460

cgtcccgtca cactagtagc ctacgaaaga gtggcaacag gatccgtcgt tacggtcgct     2520

ggggtgagta acttcgagct gattccaaat cctgaactag caaagaacct ggttacagaa     2580

tacggccgat ttgacccagg agccatgaac tacacaaaat tgatactgag tgagagggac     2640

cgtcttggca tcaagaccgt ctggccaaca agggagtaca ctgattttcg tgagtacttc     2700

atggaggtgg ccgacctcaa ctctcccctg aagattgcag gagcatttgg cttcaaagac     2760

ataatccggg ctataaggag gtaagcttga tctagagcgg ccgcggggat ccagacatga     2820

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta     2880

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag     2940

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt     3000

tttcggatcc tctagagtcg acaattattt catttaataa catatagccc aaagacctct     3060

atgaacattt agtttcccgt atactcaacg gcgcgtgtac acacaagggc gaattccaca     3120

gtggatatca agcttaatta agtaccgagc tcgaattggc gcgccaggtc aattccctgg     3180

cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta     3240

gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg     3300

tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg     3360

caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg     3420

ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag     3480

atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggcgcgccgg     3540

atccatgggc cccagacctt ctaccaagaa cccagtacct atgatgctga ctgtccgagt     3600

cgcgctggta ctgagttgca tctgtccggc aaactccatt gatggcaggc ctcttgcggc     3660

tgcaggaatt gtggttacag gagacaaagc cgtcaacata tacacctcat cccagacagg     3720

atcaatcata gttaagctcc tcccgaatct gcccaaggat aaggaggcat gtgcgaaagc     3780

ccccttggat gcatacaaca ggacattgac cactttgctc accccccttg gtgactctat     3840

ccgtaggata caagagtctg tgactacatc tggagggggg agacaggggc gccttatagg     3900

cgccattatt ggcggtgtgg ctcttggggt tgcaactgcc gcacaaataa cagcggccgc     3960

agctctgata caagccaaac aaaatgctgc caacatcctc cgacttaaag agagcattgc     4020

cgcaaccaat gaggctgtgc atgaggtcac tgacggatta tcgcaactag cagtggcagt     4080

tgggaagatg cagcagtttg ttaatgacca atttaataaa acagctcagg aattagactg     4140

catcaaaatt gcacagcaag ttggtgtaga gctcaacctg tacctaaccg aattgactac     4200

agtattcgga ccacaaatca cttcacctgc tttaaacaag ctgactattc aggcacttta     4260

caatctagct ggtggaaata tggattactt attgactaag ttaggtgtag ggaacaatca     4320

actcagctca ttaatcggta gcggcttaat caccggtaac cctattctat acgactcaca     4380

gactcaactc ttgggtatac aggtaactct accttcagtc gggaacctaa ataatatgcg     4440

tgccacctac ttggaaacct tatccgtaag cacaaccagg ggatttgcct cggcacttgt     4500

cccaaaagtg gtgacacagg tcggttctgt gatagaagaa cttgacacct catactgtat     4560

agaaactgac ttagatttat attgtacaag aatagtaacg ttccctatgt cccctggtat     4620

ttattcctgc ttgagcggca atacgtcggc ctgtatgtac tcaaagaccg aaggcgcact     4680

tactacacca tacatgacta tcaaaggttc agtcatcgcc aactgcaaga tgacaacatg     4740

tagatgtgta aaccccccgg gtatcatatc gcaaaactat ggagaagccg tgtctctaat     4800

agataaacaa tcatgcaatg ttttatcctt aggcgggata actttaaggc tcagtgggga     4860

attcgatgta acttatcaga agaatatctc aatacaagat tctcaagtaa taataacagg     4920

caatcttgat atctcaactg agcttgggaa tgtcaacaac tcgatcagta atgctttgaa     4980

taagttagag gaaagcaaca gaaaactaga caaagtcaat gtcaaactga ctagcacatc     5040

tgctctcatt acctatatcg ttttgactat catatctctt gtttttggta tacttagccc     5100

gattctagca tgctacctaa tgtacaagca aaaggcgcaa caaaagacct tattatggct     5160

tgggaataat actctagatc agatgagagc cactacaaaa atgtgaggat ctctcgagga     5220

attctagatc ccacgtcact attgtatact ctatattata ctctatgtta tactctgtaa     5280

tcctactcaa taaacgtgtc acgcctgtga aaccgtacta agtctcccgt gtcttcttat     5340

caccatcagg tgacatcctc gcccaggctg tcaatcatgc cggtatcgat tccagtagca     5400

ccggccccac gctgacaacc cactcttgca gcgttagcag cgcccctctt aacaagccga     5460

cccccaccag cgtcgcggtt actaacactc ctctcccc                             5498


<210>  2
<211>  2459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette 3


<220>
<221>  promoter
<222>  (20)..(701)
<223>  PRV gB gene promoter, extended

<220>
<221>  gene
<222>  (713)..(2395)
<223>  AIV HA H9 gene, synthetic, codon optimised

<220>
<221>  terminator
<222>  (2404)..(2458)
<223>  FHV1 Us9 gene terminator

<400>  2
agcttaatta aggcgcgcca gatctcgctg ctgcacacgt acgtggcggt ggccgccggg       60

ttccgcgcac ggcgcgcgtt ctgcgaggcc gccgcgcgcg cgggcaccgt cgtggacgag      120

cgcgagacgg gctgcttcga cgcgcacagc ttcatgaagg ccacggtgca gcgccacccc      180

gtggacgccg cgctcctccc ggcgctcacg cacaagttct tcgagctcgt caacgggccg      240

ctcttcgcgc acgacacgca cgccttcgcc cagtccccca acacggcgct ctactttgcg      300

gtggagaacg tgggcctcct gccgcacctg aaggaggagc tggcgcgctt catggtggcc      360

cgcgattggt gcgtcagtga gttccgcggc ttctaccgct tccagacggc cggcgtaacc      420

gccacccagc ggcaggcctg gcgatatatc cgcgagctgg tgctggcggt tgcagtcttc      480

aggtccgtct tccactgcgg ggacgtcgag gtcctccgcg cggatcgctt cgccggacgc      540

gacgggctgt acctgaccta cgaggcgtct tgccccgctg gtggcggtct ttggcgcggg      600

ccccgcgggc atcggcccgg gcaccacggc ggtgctggcc tcggacgtct ttggcctgct      660

ccacaccacg ctgctgctgc gcggggcgcc gtcgcgctag agatctaaag ccatggagac      720

catctccctg atgactatcc tgctcgtcgt gaccacctcc aacgccgaca agatttgcat      780

cggtcaccag tccaccaact ctaccgagac cgtggacacc ctgaccgaga ctaacgtccc      840

tgtcacccac gccaaggagc tgctgcacac tgagcacaac ggaatgctct gcgctaccaa      900

cctcggtcac ccactcatcc tggacacctg cactatcgag ggtctcatct acggcaaccc      960

ctcttgcgac ctgctcctgg gaggtcgtga gtggtcctac atcgtcgagc gtccttccgc     1020

tgtgaacggc acttgctacc ccggtaacgt ggagaacctc gaggagctcc gtactctctt     1080

ctcctcttcc tccagctacc agcgtatcca aatcttcccc gacacgatct ggaacgtgac     1140

ctacaccggt acctccaagt cctgcagcga ctctttctac cgcaacatgc gctggctgac     1200

ccagaagaac ggactgtacc ctgtgcagga cgctcagtac accaacaacc gcggaaagga     1260

catcctgttc gtctggggta tccaccaccc tcccactgac accgctcaga ccaacctgta     1320

cactcgcacc gacactacca ctagcgtcac taccgagaac ctggaccgca ctttcaagcc     1380

actgatcggt cctcgtcctc tggtgaacgg tctgatcggc aggatcaact actactggag     1440

cgtgctcaag cccggacaga ctctgagggt gcgtagcaac ggcaacctga tcgccccttg     1500

gttcggtcac gtgctgtctg gagagtctca cggacgtatc ctgaagaccg acctgaactc     1560

cggtaactgc gtggtccagt gccagactga gaagggtggc ctcaactcca ctctgccctt     1620

ccacaacatc tccaagtacg ccttcggcac ctgccccaag tacatcggtg tgaagtccct     1680

gaagctcgct atcggactgc gtaacgtccc tgctaggtcc agcaggggtc tgttcggcgc     1740

tatcgctggt ttcatcgagg gtggatggcc tggtctggtg gctggttggt acggcttcca     1800

gcactctaac gaccagggtg tcggaatggc cgctgaccgt gactctactc agaaggccgt     1860

cgacaagatc acctccaagg tgaacaacat cgtcgacaag atgaacaagc agtacgagat     1920

catcgaccac gagttctccg aggtggagac tcgcctcaac atgatcaaca acaagatcga     1980

cgaccagatc caggacgtct gggcttacaa cgctgagctc ctggtgctcc tcgagaacca     2040

gaagactctg gacgagcacg acgccaacgt caacaacctc tacaacaagg tgaagcgtgc     2100

cctgggctcc aacgctatgg aggacggcaa gggttgcttc gagctctacc acaagtgcga     2160

cgaccagtgc atggagacca tcaggaacgg gacctacaac cgccgtaagt acaaggagga     2220

gagcaggctg gagcgtcaga agatcgaggg cgtcaagctg gagtctgagg gcacctacaa     2280

gatcctgact atctactcca ccgtcgcctc tagcctcgtg ctcgctatgg gcttcgctgc     2340

cttcctgttc tgggccatgt ccaacggttc ctgccgttgc aacatctgca tctaattaat     2400

taacaataaa catagcatac gttatgacat ggtctaccgc gtcttatatg gggacgaca      2459


<210>  3
<211>  20
<212>  DNA
<213>  Herpesvirus of turkeys


<220>
<221>  primer
<222>  (1)..(19)
<223>  guide RNA for UL40-41 insertion

<400>  3
acctaaagta cacgtgaatc                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Herpesvirus of turkeys


<220>
<221>  primer
<222>  (1)..(20)
<223>  guide RNA for UL44-45 insertion

<400>  4
acatcgggac gtacatcatg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Herpesvirus of turkeys


<220>
<221>  primer
<222>  (1)..(20)
<223>  guideRNA for UL47-48 insertion

<400>  5
tggcggttac aatttccacg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Herpesvirus of turkeys


<220>
<221>  primer
<222>  (1)..(20)
<223>  guide RNA for UL54-LORF4 insertion

<400>  6
ttagatttcc ggacagcctg                                                   20


