                         SEQUENCE LISTING

<110>  Evolva SA
       Schfer, Astrid
       Malca, Sumire Honda
       Chang, Yiming
 
<120>  Host Cells and their Use for Producing a Monosaccharide or 
       Monosaccharide Precursor

<130>  P2638WO00

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  249
<212>  PRT
<213>  E. aerogenes

<400>  1

Met Asn His Ser Val Ser Ser Met Asn Thr Ser Leu Ser Gly Lys Val 
1               5                   10                  15      


Ala Ala Val Thr Gly Ala Ala Ser Gly Ile Gly Leu Glu Cys Ala Lys 
            20                  25                  30          


Thr Leu Leu Gly Ala Gly Ala Lys Val Val Leu Ile Asp Arg Glu Gly 
        35                  40                  45              


Glu Lys Leu Asn Lys Ile Val Ala Glu Leu Gly Glu Asn Ala Phe Ala 
    50                  55                  60                  


Leu Gln Val Asp Leu Met Gln Gly Glu Gln Val Asp Lys Ile Ile Asp 
65                  70                  75                  80  


Gly Ile Leu Gln Leu Ala Gly Arg Leu Asp Ile Phe His Ala Asn Ala 
                85                  90                  95      


Gly Ala Tyr Ile Gly Gly Pro Val Ala Glu Gly Asp Pro Asp Val Trp 
            100                 105                 110         


Asp Arg Val Leu His Leu Asn Thr Asn Ala Ala Phe Arg Cys Val Arg 
        115                 120                 125             


Ser Val Leu Pro His Met Ile Ala Gln Lys Ser Gly Asp Ile Ile Phe 
    130                 135                 140                 


Thr Ser Ser Ile Ala Gly Val Val Pro Val Ile Trp Glu Pro Ile Tyr 
145                 150                 155                 160 


Thr Ala Ser Lys Phe Ala Val Gln Ala Phe Val His Thr Thr Arg Arg 
                165                 170                 175     


Gln Val Ser Gln His Gly Val Arg Val Gly Ala Val Leu Pro Gly Pro 
            180                 185                 190         


Val Val Thr Ala Leu Leu Asp Asp Trp Pro Lys Glu Lys Met Glu Glu 
        195                 200                 205             


Ala Leu Ala Asn Gly Ser Leu Met Gln Pro Ile Glu Val Ala Glu Ser 
    210                 215                 220                 


Val Leu Phe Met Val Thr Arg Ser Lys Asn Val Thr Val Arg Asp Leu 
225                 230                 235                 240 


Val Ile Leu Pro Asn Ser Val Asp Leu 
                245                 


<210>  2
<211>  747
<212>  DNA
<213>  E. aerogenes

<400>  2
atgaaccact ctgtttcttc tatgaacacc tctttgtctg gtaaggttgc tgctgttacc       60

ggtgctgctt ctggtatcgg tttggagtgt gctaagacct tgttgggtgc tggtgctaag      120

gttgttttga tcgacagaga gggtgagaag ttgaacaaga tcgttgctga gttgggtgag      180

aacgctttcg ctttgcaggt tgacttgatg cagggtgagc aggttgacaa gatcatcgac      240

ggtatcttgc agttggctgg tagattggac atcttccacg ctaacgctgg tgcttacatc      300

ggtggtccag ttgctgaggg tgacccagac gtttgggaca gagttttgca cttgaacacc      360

aacgctgctt tcagatgtgt tagatctgtt ttgccacaca tgatcgctca gaagtctggt      420

gacatcatct tcacctcttc tatcgctggt gttgttccag ttatctggga gccaatctac      480

accgcttcta agttcgctgt tcaggctttc gttcacacca ccagaagaca ggtttctcag      540

cacggtgtta gagttggtgc tgttttgcca ggtccagttg ttaccgcttt gttggacgac      600

tggccaaagg agaagatgga ggaggctttg gctaacggtt ctttgatgca gccaatcgag      660

gttgctgagt ctgttttgtt catggttacc agatctaaga acgttaccgt tagagacttg      720

gttatcttgc caaactctgt tgacttg                                          747


<210>  3
<211>  249
<212>  PRT
<213>  E. coli

<400>  3

Met Asn His Ser Val Pro Ser Met Asn Thr Ser Leu Asn Gly Lys Val 
1               5                   10                  15      


Ala Ala Ile Thr Gly Ala Ala Ser Gly Ile Gly Leu Gln Cys Ala Lys 
            20                  25                  30          


Thr Leu Leu Glu Ala Gly Ala Lys Val Val Leu Ile Asp Arg Glu Gly 
        35                  40                  45              


Glu Lys Leu Gln Lys Ile Val Val Glu Leu Gly Glu Asn Ala Tyr Ala 
    50                  55                  60                  


Leu Gln Ile Asp Leu Phe Asn Gly Glu Gln Val Asp Ser Met Leu Ala 
65                  70                  75                  80  


Arg Ile Val Glu Leu Ala Gly Gly Leu Asp Ile Phe His Ala Asn Ala 
                85                  90                  95      


Gly Ala Tyr Ile Gly Gly Pro Val Ala Glu Gly Asp Pro Asp Ile Trp 
            100                 105                 110         


Asp Arg Val Leu Asn Leu Asn Ile Asn Ala Ala Phe Arg Cys Val Arg 
        115                 120                 125             


Ala Val Leu Pro His Met Ile Ala Gln Lys Ser Gly Asp Ile Ile Phe 
    130                 135                 140                 


Thr Ser Ser Ile Ala Gly Val Val Pro Val Ile Trp Glu Pro Ile Tyr 
145                 150                 155                 160 


Thr Ala Ser Lys Phe Ala Val Gln Ala Phe Val His Thr Thr Arg Arg 
                165                 170                 175     


Gln Val Ser Gln Tyr Gly Ile Arg Val Gly Ala Val Leu Pro Gly Pro 
            180                 185                 190         


Val Val Thr Ala Leu Leu Asp Asp Trp Pro Lys Ala Lys Met Asp Glu 
        195                 200                 205             


Ala Leu Ala Asn Gly Ser Leu Met Gln Pro Ile Glu Val Ala Glu Ser 
    210                 215                 220                 


Val Leu Phe Met Val Thr Arg Ser Lys Asn Val Thr Val Arg Asp Leu 
225                 230                 235                 240 


Val Ile Leu Pro Asn Ser Val Asp Leu 
                245                 


<210>  4
<211>  747
<212>  DNA
<213>  E. coli

<400>  4
atgaaccact ctgttccatc tatgaacacc tctttgaacg gtaaggttgc tgctatcacc       60

ggtgctgctt ctggtatcgg tttgcagtgt gctaagacct tgttggaggc tggtgctaag      120

gttgttttga tcgacagaga gggtgagaag ttgcagaaga tcgttgttga gttgggtgag      180

aacgcttacg ctttgcagat cgacttgttc aacggtgagc aggttgactc tatgttggct      240

agaatcgttg agttggctgg tggtttggac atcttccacg ctaacgctgg tgcttacatc      300

ggtggtccag ttgctgaggg tgacccagac atctgggaca gagttttgaa cttgaacatc      360

aacgctgctt tcagatgtgt tagagctgtt ttgccacaca tgatcgctca gaagtctggt      420

gacatcatct tcacctcttc tatcgctggt gttgttccag ttatctggga gccaatctac      480

accgcttcta agttcgctgt tcaggctttc gttcacacca ccagaagaca ggtttctcag      540

tacggtatca gagttggtgc tgttttgcca ggtccagttg ttaccgcttt gttggacgac      600

tggccaaagg ctaagatgga cgaggctttg gctaacggtt ctttgatgca gccaatcgag      660

gttgctgagt ctgttttgtt catggttacc agatctaaga acgttaccgt tagagacttg      720

gttatcttgc caaactctgt tgacttg                                          747


<210>  5
<211>  242
<212>  PRT
<213>  R. sphaeroides

<400>  5

Met Ala Glu Leu Met Gln Gly Lys Val Ala Ala Ile Thr Gly Ala Ala 
1               5                   10                  15      


Ser Gly Ile Gly Leu Glu Cys Ala Arg Thr Leu Val Ala Glu Gly Ala 
            20                  25                  30          


Thr Val Val Leu Ile Asp Arg Ala Glu Asp Arg Leu Lys Ala Leu Cys 
        35                  40                  45              


Ala Glu Ile Gly Pro Arg Ala Leu Pro Leu Val Val Asp Leu Leu Asp 
    50                  55                  60                  


Gly Pro Gln Val Ser Gly Met Leu Pro Arg Ile Leu Glu Leu Ala Gly 
65                  70                  75                  80  


Ser Leu Asp Ile Phe His Ala Asn Ala Gly Ala Tyr Ile Gly Gly Gln 
                85                  90                  95      


Val Ala Glu Gly Asp Pro Asp Ala Trp Asp Arg Met Leu Asn Leu Asn 
            100                 105                 110         


Ile Asn Ala Ala Phe Arg Ser Val His Ala Val Leu Pro Tyr Met Ile 
        115                 120                 125             


Glu Arg Lys Ser Gly Asp Ile Leu Phe Thr Ser Ser Val Ala Gly Val 
    130                 135                 140                 


Val Pro Val Val Trp Glu Pro Ile Tyr Thr Ala Ser Lys Phe Ala Val 
145                 150                 155                 160 


Gln Ala Phe Val His Ser Thr Arg Arg Gln Val Ala Pro His Gly Val 
                165                 170                 175     


Arg Val Gly Ala Val Leu Pro Gly Pro Val Val Thr Ala Leu Leu Asp 
            180                 185                 190         


Asp Trp Pro Lys Ala Lys Met Glu Glu Ala Leu Ala Asn Gly Ser Leu 
        195                 200                 205             


Met Gln Pro Lys Glu Val Ala Glu Ala Val Leu Phe Met Leu Ser Arg 
    210                 215                 220                 


Pro Lys Gly Val Val Ile Arg Asp Leu Val Ile Leu Pro His Ser Val 
225                 230                 235                 240 


Asp Ile 
        


<210>  6
<211>  726
<212>  DNA
<213>  R. sphaeroides

<400>  6
atggctgagt tgatgcaggg taaggttgct gctatcaccg gtgctgcttc tggtatcggt       60

ttggagtgtg ctagaacctt ggttgctgag ggtgctaccg ttgttttgat cgacagagct      120

gaggacagat tgaaggcttt gtgtgctgag atcggtccaa gagctttgcc attggttgtt      180

gacttgttgg acggtccaca ggtttctggt atgttgccaa gaatcttgga gttggctggt      240

tctttggaca tcttccacgc taacgctggt gcttacatcg gtggtcaggt tgctgagggt      300

gacccagacg cttgggacag aatgttgaac ttgaacatca acgctgcttt cagatctgtt      360

cacgctgttt tgccatacat gatcgagaga aagtctggtg acatcttgtt cacctcttct      420

gttgctggtg ttgttccagt tgtttgggag ccaatctaca ccgcttctaa gttcgctgtt      480

caggctttcg ttcactctac cagaagacag gttgctccac acggtgttag agttggtgct      540

gttttgccag gtccagttgt taccgctttg ttggacgact ggccaaaggc taagatggag      600

gaggctttgg ctaacggttc tttgatgcag ccaaaggagg ttgctgaggc tgttttgttc      660

atgttgtcta gaccaaaggg tgttgttatc agagacttgg ttatcttgcc acactctgtt      720

gacatc                                                                 726


<210>  7
<211>  249
<212>  PRT
<213>  E. aerogenes

<400>  7

Met Asn His Ser Val Ser Ser Met Asn Thr Ser Leu Ser Gly Lys Val 
1               5                   10                  15      


Ala Ala Val Thr Gly Ala Ala Ser Gly Ile Gly Leu Glu Cys Ala Lys 
            20                  25                  30          


Thr Leu Leu Gly Ala Gly Ala Lys Val Val Leu Ile Ser Arg Glu Gly 
        35                  40                  45              


Glu Lys Leu Asn Lys Ile Val Ala Glu Leu Gly Glu Asn Ala Phe Ala 
    50                  55                  60                  


Leu Gln Val Asp Leu Met Gln Gly Glu Gln Val Asp Lys Ile Ile Asp 
65                  70                  75                  80  


Gly Ile Leu Gln Leu Ala Gly Arg Leu Asp Ile Phe His Ala Asn Ala 
                85                  90                  95      


Gly Ala Tyr Ile Gly Gly Pro Val Ala Glu Gly Asp Pro Asp Val Trp 
            100                 105                 110         


Asp Arg Val Leu His Leu Asn Thr Asn Ala Ala Phe Arg Cys Val Arg 
        115                 120                 125             


Ser Val Leu Pro His Met Ile Ala Gln Lys Ser Gly Asp Ile Ile Phe 
    130                 135                 140                 


Thr Ser Ser Ile Ala Gly Val Val Pro Val Ile Trp Glu Pro Ile Tyr 
145                 150                 155                 160 


Thr Ala Ser Lys Phe Ala Val Gln Ala Phe Val His Thr Thr Arg Arg 
                165                 170                 175     


Gln Val Ser Gln His Gly Val Arg Val Gly Ala Val Leu Pro Gly Pro 
            180                 185                 190         


Val Val Thr Ala Leu Leu Asp Asp Trp Pro Lys Glu Lys Met Glu Glu 
        195                 200                 205             


Ala Leu Ala Asn Gly Ser Leu Met Gln Pro Ile Glu Val Ala Glu Ser 
    210                 215                 220                 


Val Leu Phe Met Val Thr Arg Ser Lys Asn Val Thr Val Arg Asp Leu 
225                 230                 235                 240 


Val Ile Leu Pro Asn Ser Val Asp Leu 
                245                 


<210>  8
<211>  747
<212>  DNA
<213>  E. aerogenes

<400>  8
atgaaccact ctgtttcttc tatgaacacc tctttgtctg gtaaggttgc tgctgttacc       60

ggtgctgctt ctggtatcgg tttggagtgt gctaagacct tgttgggtgc tggtgctaag      120

gttgttttga tctctagaga gggtgagaag ttgaacaaga tcgttgctga gttgggtgag      180

aacgctttcg ctttgcaggt tgacttgatg cagggtgagc aggttgacaa gatcatcgac      240

ggtatcttgc agttggctgg tagattggac atcttccacg ctaacgctgg tgcttacatc      300

ggtggtccag ttgctgaggg tgacccagac gtttgggaca gagttttgca cttgaacacc      360

aacgctgctt tcagatgtgt tagatctgtt ttgccacaca tgatcgctca gaagtctggt      420

gacatcatct tcacctcttc tatcgctggt gttgttccag ttatctggga gccaatctac      480

accgcttcta agttcgctgt tcaggctttc gttcacacca ccagaagaca ggtttctcag      540

cacggtgtta gagttggtgc tgttttgcca ggtccagttg ttaccgcttt gttggacgac      600

tggccaaagg agaagatgga ggaggctttg gctaacggtt ctttgatgca gccaatcgag      660

gttgctgagt ctgttttgtt catggttacc agatctaaga acgttaccgt tagagacttg      720

gttatcttgc caaactctgt tgacttg                                          747


<210>  9
<211>  249
<212>  PRT
<213>  E. coli

<400>  9

Met Asn His Ser Val Pro Ser Met Asn Thr Ser Leu Asn Gly Lys Val 
1               5                   10                  15      


Ala Ala Ile Thr Gly Ala Ala Ser Gly Ile Gly Leu Gln Cys Ala Lys 
            20                  25                  30          


Thr Leu Leu Glu Ala Gly Ala Lys Val Val Leu Ile Ser Arg Glu Gly 
        35                  40                  45              


Glu Lys Leu Gln Lys Ile Val Val Glu Leu Gly Glu Asn Ala Tyr Ala 
    50                  55                  60                  


Leu Gln Ile Asp Leu Phe Asn Gly Glu Gln Val Asp Ser Met Leu Ala 
65                  70                  75                  80  


Arg Ile Val Glu Leu Ala Gly Gly Leu Asp Ile Phe His Ala Asn Ala 
                85                  90                  95      


Gly Ala Tyr Ile Gly Gly Pro Val Ala Glu Gly Asp Pro Asp Ile Trp 
            100                 105                 110         


Asp Arg Val Leu Asn Leu Asn Ile Asn Ala Ala Phe Arg Cys Val Arg 
        115                 120                 125             


Ala Val Leu Pro His Met Ile Ala Gln Lys Ser Gly Asp Ile Ile Phe 
    130                 135                 140                 


Thr Ser Ser Ile Ala Gly Val Val Pro Val Ile Trp Glu Pro Ile Tyr 
145                 150                 155                 160 


Thr Ala Ser Lys Phe Ala Val Gln Ala Phe Val His Thr Thr Arg Arg 
                165                 170                 175     


Gln Val Ser Gln Tyr Gly Ile Arg Val Gly Ala Val Leu Pro Gly Pro 
            180                 185                 190         


Val Val Thr Ala Leu Leu Asp Asp Trp Pro Lys Ala Lys Met Asp Glu 
        195                 200                 205             


Ala Leu Ala Asn Gly Ser Leu Met Gln Pro Ile Glu Val Ala Glu Ser 
    210                 215                 220                 


Val Leu Phe Met Val Thr Arg Ser Lys Asn Val Thr Val Arg Asp Leu 
225                 230                 235                 240 


Val Ile Leu Pro Asn Ser Val Asp Leu 
                245                 


<210>  10
<211>  747
<212>  DNA
<213>  E. coli

<400>  10
atgaaccact ctgttccatc tatgaacacc tctttgaacg gtaaggttgc tgctatcacc       60

ggtgctgctt ctggtatcgg tttgcagtgt gctaagacct tgttggaggc tggtgctaag      120

gttgttttga tctctagaga gggtgagaag ttgcagaaga tcgttgttga gttgggtgag      180

aacgcttacg ctttgcagat cgacttgttc aacggtgagc aggttgactc tatgttggct      240

agaatcgttg agttggctgg tggtttggac atcttccacg ctaacgctgg tgcttacatc      300

ggtggtccag ttgctgaggg tgacccagac atctgggaca gagttttgaa cttgaacatc      360

aacgctgctt tcagatgtgt tagagctgtt ttgccacaca tgatcgctca gaagtctggt      420

gacatcatct tcacctcttc tatcgctggt gttgttccag ttatctggga gccaatctac      480

accgcttcta agttcgctgt tcaggctttc gttcacacca ccagaagaca ggtttctcag      540

tacggtatca gagttggtgc tgttttgcca ggtccagttg ttaccgcttt gttggacgac      600

tggccaaagg ctaagatgga cgaggctttg gctaacggtt ctttgatgca gccaatcgag      660

gttgctgagt ctgttttgtt catggttacc agatctaaga acgttaccgt tagagacttg      720

gttatcttgc caaactctgt tgacttg                                          747


<210>  11
<211>  242
<212>  PRT
<213>  R. sphaeroides

<400>  11

Met Ala Glu Leu Met Gln Gly Lys Val Ala Ala Ile Thr Gly Ala Ala 
1               5                   10                  15      


Ser Gly Ile Gly Leu Glu Cys Ala Arg Thr Leu Val Ala Glu Gly Ala 
            20                  25                  30          


Thr Val Val Leu Ile Ser Arg Ala Glu Asp Arg Leu Lys Ala Leu Cys 
        35                  40                  45              


Ala Glu Ile Gly Pro Arg Ala Leu Pro Leu Val Val Asp Leu Leu Asp 
    50                  55                  60                  


Gly Pro Gln Val Ser Gly Met Leu Pro Arg Ile Leu Glu Leu Ala Gly 
65                  70                  75                  80  


Ser Leu Asp Ile Phe His Ala Asn Ala Gly Ala Tyr Ile Gly Gly Gln 
                85                  90                  95      


Val Ala Glu Gly Asp Pro Asp Ala Trp Asp Arg Met Leu Asn Leu Asn 
            100                 105                 110         


Ile Asn Ala Ala Phe Arg Ser Val His Ala Val Leu Pro Tyr Met Ile 
        115                 120                 125             


Glu Arg Lys Ser Gly Asp Ile Leu Phe Thr Ser Ser Val Ala Gly Val 
    130                 135                 140                 


Val Pro Val Val Trp Glu Pro Ile Tyr Thr Ala Ser Lys Phe Ala Val 
145                 150                 155                 160 


Gln Ala Phe Val His Ser Thr Arg Arg Gln Val Ala Pro His Gly Val 
                165                 170                 175     


Arg Val Gly Ala Val Leu Pro Gly Pro Val Val Thr Ala Leu Leu Asp 
            180                 185                 190         


Asp Trp Pro Lys Ala Lys Met Glu Glu Ala Leu Ala Asn Gly Ser Leu 
        195                 200                 205             


Met Gln Pro Lys Glu Val Ala Glu Ala Val Leu Phe Met Leu Ser Arg 
    210                 215                 220                 


Pro Lys Gly Val Val Ile Arg Asp Leu Val Ile Leu Pro His Ser Val 
225                 230                 235                 240 


Asp Ile 
        


<210>  12
<211>  726
<212>  DNA
<213>  R. sphaeroides

<400>  12
atggctgagt tgatgcaggg taaggttgct gctatcaccg gtgctgcttc tggtatcggt       60

ttggagtgtg ctagaacctt ggttgctgag ggtgctaccg ttgttttgat ctctagagct      120

gaggacagat tgaaggcttt gtgtgctgag atcggtccaa gagctttgcc attggttgtt      180

gacttgttgg acggtccaca ggtttctggt atgttgccaa gaatcttgga gttggctggt      240

tctttggaca tcttccacgc taacgctggt gcttacatcg gtggtcaggt tgctgagggt      300

gacccagacg cttgggacag aatgttgaac ttgaacatca acgctgcttt cagatctgtt      360

cacgctgttt tgccatacat gatcgagaga aagtctggtg acatcttgtt cacctcttct      420

gttgctggtg ttgttccagt tgtttgggag ccaatctaca ccgcttctaa gttcgctgtt      480

caggctttcg ttcactctac cagaagacag gttgctccac acggtgttag agttggtgct      540

gttttgccag gtccagttgt taccgctttg ttggacgact ggccaaaggc taagatggag      600

gaggctttgg ctaacggttc tttgatgcag ccaaaggagg ttgctgaggc tgttttgttc      660

atgttgtcta gaccaaaggg tgttgttatc agagacttgg ttatcttgcc acactctgtt      720

gacatc                                                                 726


<210>  13
<211>  32
<212>  DNA
<213>  oligo

<400>  13
cggaccctta ttaaactaaa aggcgcgcca aa                                     32


<210>  14
<211>  33
<212>  DNA
<213>  oligo

<400>  14
cttctcaccc tctctagaga tcaaaacaac ctt                                    33


<210>  15
<211>  33
<212>  DNA
<213>  oligo

<400>  15
aaggttgttt tgatctctag agagggtgag aag                                    33


<210>  16
<211>  33
<212>  DNA
<213>  oligo

<400>  16
tcatatcttt ctagattctc ttcgagagca tgc                                    33


<210>  17
<211>  33
<212>  DNA
<213>  oligo

<400>  17
tctgtcctca gctctagaga tcaaaacaac ggt                                    33


<210>  18
<211>  33
<212>  DNA
<213>  oligo

<400>  18
accgttgttt tgatctctag agctgaggac aga                                    33


<210>  19
<211>  30
<212>  DNA
<213>  oligo

<400>  19
aaagggctgc agggatccgt agaaatcttg                                        30


<210>  20
<211>  30
<212>  DNA
<213>  oligo

<400>  20
aaaggggtcg acttgctcgt ggtcggaaat                                        30


<210>  21
<211>  30
<212>  DNA
<213>  oligo

<400>  21
aaagggccat ggttctactg tgctaaggcc                                        30


<210>  22
<211>  30
<212>  DNA
<213>  oligo

<400>  22
aaagggacta gtagaatgac acggccgaca                                        30


