                         SEQUENCE LISTING

<110>  Keygene N.V.
       Stichting Wageningen Research
 
<120>  germacrene A synthase mutants

<130>  P6090372pct

<150>  EP 19217865.5
<151>  2019-12-19

<150>  EP 20180022.4
<151>  2020-06-15

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  558
<212>  PRT
<213>  Cichorium intybus

<400>  1

Met Ala Ala Val Glu Ala Asn Gly Thr Leu Gln Thr Asn Thr Lys Thr 
1               5                   10                  15      


Thr Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp Gly 
            20                  25                  30          


Asp Arg Phe Leu Ser Phe Ser Leu Asp Thr Lys Glu Leu Glu Gly Tyr 
        35                  40                  45              


Ala Lys Ala Met Glu Glu Pro Lys Glu Glu Leu Arg Lys Leu Ile Val 
    50                  55                  60                  


Asp Pro Thr Met Asp Ser Asn Lys Lys Leu Ser Leu Ile Tyr Ser Val 
65                  70                  75                  80  


His Arg Leu Gly Leu Ala Tyr Leu Phe Leu Gln Glu Ile Glu Ala Gln 
                85                  90                  95      


Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Asp Glu Phe 
            100                 105                 110         


Asp Leu Tyr Thr Thr Ser Ile Asn Phe Gln Val Phe Arg His Leu Gly 
        115                 120                 125             


His Lys Leu Pro Cys Asp Val Phe Asn Lys Phe Lys Asp Ser Ser Leu 
    130                 135                 140                 


Gly Thr Phe Lys Glu Ser Ile Thr Asn Asp Val Lys Gly Met Leu Gly 
145                 150                 155                 160 


Leu Tyr Glu Ser Ala Gln Leu Arg Leu Arg Gly Glu Pro Ile Leu Asp 
                165                 170                 175     


Glu Ala Ser Ala Phe Thr Glu Thr Gln Leu Lys Ser Val Val Asn Thr 
            180                 185                 190         


Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg Pro 
        195                 200                 205             


Phe His Gln Gly Met Pro Met Val Glu Ala Arg Met Tyr Phe Ser Asn 
    210                 215                 220                 


Tyr Asp Glu Glu Cys Ser Thr His Glu Ser Leu Leu Lys Leu Ala Lys 
225                 230                 235                 240 


Leu His Phe Asn Tyr Leu Gln Leu Gln Gln Lys Glu Glu Leu Arg Ile 
                245                 250                 255     


Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro Tyr 
            260                 265                 270         


Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu Tyr 
        275                 280                 285             


Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile Thr 
    290                 295                 300                 


Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr Ile 
305                 310                 315                 320 


Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Ile Ser 
                325                 330                 335     


Ala Met Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile Leu 
            340                 345                 350         


Leu Asp Glu Tyr Ala Glu Leu Glu Lys Gln Leu Ala Lys Glu Gly Arg 
        355                 360                 365             


Ala Lys Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala Arg 
    370                 375                 380                 


Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala Ser 
385                 390                 395                 400 


Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn Val 
                405                 410                 415     


Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Met Val Ser Glu Asp 
            420                 425                 430         


Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser Glu 
        435                 440                 445             


Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu Arg 
    450                 455                 460                 


Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ser Tyr Ile Lys Thr Tyr 
465                 470                 475                 480 


Gly Val Thr Glu Lys Glu Ala Ile Asp Glu Leu Asn Lys Met Ile Glu 
                485                 490                 495     


Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu Val 
            500                 505                 510         


Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile Asp 
        515                 520                 525             


Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr Met 
    530                 535                 540                 


Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Ser Pro Met 
545                 550                 555             


<210>  2
<211>  558
<212>  PRT
<213>  Cichorium intybus

<400>  2

Met Ala Ala Val Glu Ala Asn Gly Thr Leu Gln Ala Asn Thr Lys Thr 
1               5                   10                  15      


Thr Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp Gly 
            20                  25                  30          


Asp Arg Phe Leu Ser Phe Ser Leu Asp Thr Lys Glu Leu Glu Gly Tyr 
        35                  40                  45              


Ala Lys Ala Met Glu Glu Pro Lys Glu Glu Leu Arg Lys Leu Ile Val 
    50                  55                  60                  


Asp Pro Thr Met Asp Ser Lys Lys Lys Leu Ser Leu Ile Tyr Ser Val 
65                  70                  75                  80  


His Arg Leu Gly Leu Ala Tyr Leu Phe Leu Gln Glu Ile Glu Ala Gln 
                85                  90                  95      


Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Asp Glu Phe 
            100                 105                 110         


Asp Leu Tyr Thr Thr Ser Ile Asn Phe Gln Val Phe Arg His Leu Gly 
        115                 120                 125             


His Lys Leu Pro Cys Asp Val Phe Asn Lys Phe Lys Asp Ser Ser Leu 
    130                 135                 140                 


Gly Thr Phe Lys Glu Ser Ile Thr Asn Asp Val Lys Gly Met Leu Gly 
145                 150                 155                 160 


Leu Tyr Glu Ser Ala Gln Leu Arg Leu Arg Gly Glu Pro Ile Leu Asp 
                165                 170                 175     


Glu Ala Ser Ala Phe Thr Glu Thr Gln Leu Lys Ser Val Val Asn Thr 
            180                 185                 190         


Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg Pro 
        195                 200                 205             


Phe His Gln Gly Met Pro Met Val Glu Ala Arg Met Tyr Phe Ser Asn 
    210                 215                 220                 


Tyr Asp Glu Glu Cys Ser Thr His Glu Ser Leu Leu Lys Leu Ala Lys 
225                 230                 235                 240 


Leu His Phe Asn Tyr Leu Gln Leu Gln Gln Lys Glu Glu Leu Arg Ile 
                245                 250                 255     


Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro Tyr 
            260                 265                 270         


Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu Tyr 
        275                 280                 285             


Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile Thr 
    290                 295                 300                 


Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr Ile 
305                 310                 315                 320 


Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Ile Ser 
                325                 330                 335     


Ala Met Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile Leu 
            340                 345                 350         


Leu Asp Glu Tyr Ala Glu Leu Glu Lys Gln Leu Ala Lys Glu Gly Arg 
        355                 360                 365             


Ala Lys Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala Arg 
    370                 375                 380                 


Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala Ser 
385                 390                 395                 400 


Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn Val 
                405                 410                 415     


Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Met Val Ser Glu Asp 
            420                 425                 430         


Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser Glu 
        435                 440                 445             


Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu Arg 
    450                 455                 460                 


Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ser Tyr Ile Lys Thr Tyr 
465                 470                 475                 480 


Gly Val Thr Glu Lys Glu Ala Ile Asp Glu Leu Asn Lys Met Ile Glu 
                485                 490                 495     


Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu Val 
            500                 505                 510         


Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile Asp 
        515                 520                 525             


Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr Met 
    530                 535                 540                 


Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Ser Pro Met 
545                 550                 555             


<210>  3
<211>  558
<212>  PRT
<213>  Cichorium intybus

<400>  3

Met Ala Ala Val Glu Ala Asn Gly Thr Phe Gln Ala Asn Thr Lys Thr 
1               5                   10                  15      


Thr Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp Gly 
            20                  25                  30          


Asp Arg Phe Leu Ser Phe Ser Leu Asp Thr Thr Glu Leu Glu Gly Tyr 
        35                  40                  45              


Ala Lys Ala Met Glu Glu Pro Lys Glu Glu Val Arg Lys Leu Ile Val 
    50                  55                  60                  


Asp Pro Thr Met Asp Ser Asn Lys Lys Leu Ser Leu Ile Tyr Ser Val 
65                  70                  75                  80  


His Arg Leu Gly Leu Thr Tyr Leu Phe Leu Gln Glu Ile Glu Ala Gln 
                85                  90                  95      


Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Asp Glu Phe 
            100                 105                 110         


Asp Leu Tyr Thr Thr Ser Ile Asn Phe Gln Val Phe Arg His Leu Gly 
        115                 120                 125             


His Lys Leu Pro Cys Asp Val Phe Asn Lys Phe Lys Asp Ser Ser Ser 
    130                 135                 140                 


Gly Thr Phe Lys Glu Ser Ile Thr Asn Asp Val Lys Gly Met Leu Gly 
145                 150                 155                 160 


Leu Tyr Glu Ser Ala Gln Leu Arg Leu Arg Gly Glu Pro Ile Leu Asp 
                165                 170                 175     


Glu Ala Ser Ala Phe Thr Glu Thr Gln Leu Lys Ser Val Val Asn Thr 
            180                 185                 190         


Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg Pro 
        195                 200                 205             


Phe His Gln Gly Met Pro Met Val Glu Ala Arg Met Tyr Phe Ser Asn 
    210                 215                 220                 


Tyr Asp Glu Glu Cys Ser Thr His Glu Ser Leu Pro Lys Leu Ala Lys 
225                 230                 235                 240 


Leu His Phe Asn Tyr Leu Gln Leu Gln Gln Lys Glu Glu Leu Arg Ile 
                245                 250                 255     


Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro Tyr 
            260                 265                 270         


Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu Tyr 
        275                 280                 285             


Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile Thr 
    290                 295                 300                 


Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr Ile 
305                 310                 315                 320 


Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Ile Ser 
                325                 330                 335     


Ala Met Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile Leu 
            340                 345                 350         


Leu Asp Glu Tyr Ala Glu Leu Glu Lys Gln Leu Ala Lys Glu Gly Arg 
        355                 360                 365             


Ala Lys Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala Arg 
    370                 375                 380                 


Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala Ser 
385                 390                 395                 400 


Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn Val 
                405                 410                 415     


Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Met Val Gly Glu Asp 
            420                 425                 430         


Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser Glu 
        435                 440                 445             


Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu Arg 
    450                 455                 460                 


Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ser Tyr Ile Lys Thr Tyr 
465                 470                 475                 480 


Gly Val Thr Glu Lys Glu Ala Ile Asp Glu Leu Asn Lys Met Ile Glu 
                485                 490                 495     


Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu Val 
            500                 505                 510         


Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile Asp 
        515                 520                 525             


Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr Met 
    530                 535                 540                 


Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Ser Pro Met 
545                 550                 555             


<210>  4
<211>  558
<212>  PRT
<213>  Cichorium intybus

<400>  4

Met Ala Ala Val Glu Ala Asn Gly Thr Phe Gln Ala Asn Thr Lys Thr 
1               5                   10                  15      


Thr Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp Gly 
            20                  25                  30          


Asp Arg Phe Leu Ser Phe Ser Leu Asp Thr Thr Glu Leu Glu Gly Tyr 
        35                  40                  45              


Ala Lys Ala Met Glu Glu Pro Lys Glu Glu Val Arg Lys Leu Ile Val 
    50                  55                  60                  


Asp Pro Thr Met Asp Ser Asn Lys Lys Leu Ser Leu Ile Tyr Ser Val 
65                  70                  75                  80  


His Arg Leu Gly Leu Thr Tyr Leu Phe Leu Gln Glu Ile Glu Ala Gln 
                85                  90                  95      


Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Asp Glu Phe 
            100                 105                 110         


Asp Leu Tyr Thr Thr Ser Ile Asn Phe Gln Val Phe Arg His Leu Gly 
        115                 120                 125             


His Lys Leu Pro Cys Asp Val Phe Asn Lys Phe Lys Asp Ser Ser Ser 
    130                 135                 140                 


Gly Thr Phe Lys Glu Ser Ile Thr Asn Asp Val Lys Gly Met Leu Gly 
145                 150                 155                 160 


Leu Tyr Glu Ser Ala Gln Leu Arg Leu Arg Gly Glu Pro Ile Leu Asp 
                165                 170                 175     


Glu Ala Ser Ala Phe Thr Glu Thr Gln Leu Lys Ser Val Val Asn Thr 
            180                 185                 190         


Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg Pro 
        195                 200                 205             


Phe His Gln Gly Met Pro Met Val Glu Ala Arg Met Tyr Phe Ser Asn 
    210                 215                 220                 


Tyr Asp Glu Glu Cys Ser Thr His Glu Ser Leu Pro Lys Leu Ala Lys 
225                 230                 235                 240 


Leu His Phe Asn Tyr Leu Gln Leu Gln Gln Lys Glu Glu Leu Arg Ile 
                245                 250                 255     


Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro Tyr 
            260                 265                 270         


Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu Tyr 
        275                 280                 285             


Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile Thr 
    290                 295                 300                 


Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr Ile 
305                 310                 315                 320 


Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Ile Ser 
                325                 330                 335     


Ala Met Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile Leu 
            340                 345                 350         


Leu Asp Glu Tyr Ala Glu Leu Glu Lys Gln Leu Ala Lys Glu Gly Arg 
        355                 360                 365             


Ala Lys Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala Arg 
    370                 375                 380                 


Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala Ser 
385                 390                 395                 400 


Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn Val 
                405                 410                 415     


Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Met Val Gly Glu Asp 
            420                 425                 430         


Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser Glu 
        435                 440                 445             


Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu Arg 
    450                 455                 460                 


Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ser Tyr Ile Lys Thr Tyr 
465                 470                 475                 480 


Gly Val Thr Glu Lys Glu Ala Ile Asp Glu Leu Asn Lys Met Ile Glu 
                485                 490                 495     


Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu Val 
            500                 505                 510         


Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile Asp 
        515                 520                 525             


Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr Met 
    530                 535                 540                 


Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Ser Pro Met 
545                 550                 555             


<210>  5
<211>  559
<212>  PRT
<213>  Cichorium intybus

<400>  5

Met Ala Ala Val Glu Thr Asn Gly Thr Ile Gln Ala Lys Thr Asn Thr 
1               5                   10                  15      


Thr Ala Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp 
            20                  25                  30          


Gly Asp Arg Phe Leu Ser Phe Thr Leu Asp Asn Ser Glu Leu Glu Gly 
        35                  40                  45              


Tyr Ala Arg Ala Met Glu Glu Pro Lys Glu Asp Leu Arg Arg Leu Ile 
    50                  55                  60                  


Thr Asp Ala Thr Met Glu Ser Asn Lys Lys Leu Ser Leu Ile Tyr Ser 
65                  70                  75                  80  


Val His Arg Leu Gly Leu Thr Tyr Leu Phe Leu Gln Glu Ile Glu Ala 
                85                  90                  95      


Gln Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Ile Asp 
            100                 105                 110         


Leu Asp Leu Tyr Thr Leu Ser Ile Asn Phe Gln Val Phe Arg His Leu 
        115                 120                 125             


Gly Tyr Lys Leu Pro Cys Asp Val Phe Asn Lys Ile Lys Asp Ser Asn 
    130                 135                 140                 


Ser Gly Thr Phe Lys Glu Asp Ile Ser Thr Asp Val Lys Gly Met Leu 
145                 150                 155                 160 


Gly Leu Tyr Glu Ser Ala Gln Leu Arg Val Arg Gly Glu Ser Ile Leu 
                165                 170                 175     


Asp Glu Ala Ser Ala Phe Thr Glu Ala Lys Leu Lys Ser Val Val Asn 
            180                 185                 190         


Thr Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg 
        195                 200                 205             


Pro Phe His Gln Gly Met Pro Met Val Glu Ala Arg Leu Tyr Phe Ser 
    210                 215                 220                 


Asn Tyr Asp Glu Asp Cys Ser Thr His Glu Ser Leu Leu Lys Leu Ala 
225                 230                 235                 240 


Lys Leu His Phe Asn Tyr Leu Glu Leu Gln Gln Lys Glu Glu Leu Arg 
                245                 250                 255     


Ile Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro 
            260                 265                 270         


Tyr Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu 
        275                 280                 285             


Tyr Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile 
    290                 295                 300                 


Thr Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr 
305                 310                 315                 320 


Ile Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Met 
                325                 330                 335     


Ser Ala Ile Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile 
            340                 345                 350         


Leu Leu Asp Glu Tyr Ala Asp Leu Glu Lys Gln Leu Ala Ile Glu Gly 
        355                 360                 365             


Arg Ala Asn Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala 
    370                 375                 380                 


Arg Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala 
385                 390                 395                 400 


Ser Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn 
                405                 410                 415     


Val Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Ile Val Ser Glu 
            420                 425                 430         


Asn Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser 
        435                 440                 445             


Glu Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu 
    450                 455                 460                 


Arg Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ala Tyr Ile Lys Thr 
465                 470                 475                 480 


Tyr Gly Val Ser Glu Lys Glu Ala Ile Asp Glu Leu Lys Ile Met Ile 
                485                 490                 495     


Glu Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu 
            500                 505                 510         


Val Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile 
        515                 520                 525             


Asp Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr 
    530                 535                 540                 


Met Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Val Ser Met 
545                 550                 555                 


<210>  6
<211>  559
<212>  PRT
<213>  Cichorium intybus

<400>  6

Met Ala Ala Val Glu Thr Asn Gly Thr Ile Gln Ala Lys Thr Asn Thr 
1               5                   10                  15      


Thr Ala Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp 
            20                  25                  30          


Gly Asp Arg Phe Leu Ser Phe Thr Leu Asp Asn Ser Glu Leu Glu Gly 
        35                  40                  45              


Tyr Ala Arg Ala Met Glu Glu Pro Lys Glu Asp Leu Arg Arg Leu Ile 
    50                  55                  60                  


Thr Asp Ala Thr Met Glu Ser Asn Lys Lys Leu Ser Leu Ile Tyr Ser 
65                  70                  75                  80  


Val His Arg Leu Gly Leu Thr Tyr Leu Phe Leu Gln Glu Ile Glu Ala 
                85                  90                  95      


Gln Leu Asp Lys Leu Phe Lys Glu Phe Asn Leu Gln Asp Tyr Ile Asp 
            100                 105                 110         


Leu Asp Leu Tyr Thr Leu Ser Ile Asn Phe Gln Val Phe Arg His Leu 
        115                 120                 125             


Gly Tyr Lys Leu Pro Cys Asp Val Phe Asn Lys Ile Lys Val Ser Asn 
    130                 135                 140                 


Ser Gly Thr Phe Lys Glu Asp Ile Ser Thr Asp Val Lys Gly Met Leu 
145                 150                 155                 160 


Gly Leu Tyr Glu Ser Ala Gln Leu Arg Val Arg Gly Glu Ser Ile Leu 
                165                 170                 175     


Asp Glu Ala Ser Ala Phe Thr Glu Ala Lys Leu Lys Ser Val Val Asn 
            180                 185                 190         


Thr Leu Glu Gly Asn Leu Ala Lys Gln Val Met Gln Ser Leu Arg Arg 
        195                 200                 205             


Pro Phe His Gln Gly Met Pro Met Val Glu Ala Arg Leu Tyr Phe Ser 
    210                 215                 220                 


Asn Tyr Asp Glu Asp Cys Ser Thr His Glu Ser Leu Leu Lys Leu Ala 
225                 230                 235                 240 


Lys Leu His Phe Asn Tyr Leu Glu Leu Gln Gln Lys Glu Glu Leu Arg 
                245                 250                 255     


Ile Val Ser Lys Trp Trp Lys Asp Met Arg Phe Gln Glu Thr Thr Pro 
            260                 265                 270         


Tyr Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu Trp Ile Leu Gly Leu 
        275                 280                 285             


Tyr Phe Glu Pro Arg Tyr Ser Leu Ala Arg Ile Ile Ala Thr Lys Ile 
    290                 295                 300                 


Thr Leu Phe Leu Val Val Leu Asp Asp Thr Tyr Asp Ala Tyr Ala Thr 
305                 310                 315                 320 


Ile Glu Glu Ile Arg Leu Leu Thr Asp Ala Ile Asn Arg Trp Asp Met 
                325                 330                 335     


Ser Ala Ile Glu Gln Ile Pro Glu Tyr Ile Arg Pro Phe Tyr Lys Ile 
            340                 345                 350         


Leu Leu Asp Glu Tyr Ala Asp Leu Glu Lys Gln Leu Ala Ile Glu Gly 
        355                 360                 365             


Arg Ala Asn Ser Val Ile Ala Ser Lys Glu Ala Phe Gln Asp Ile Ala 
    370                 375                 380                 


Arg Gly Tyr Leu Glu Glu Ala Glu Trp Thr Asn Ser Gly Tyr Val Ala 
385                 390                 395                 400 


Ser Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile Thr Ser Ala Tyr Asn 
                405                 410                 415     


Val Ile Ser Lys Ser Ala Leu Val Gly Met Gly Glu Ile Val Ser Glu 
            420                 425                 430         


Asn Ala Leu Ala Trp Tyr Glu Ser His Pro Lys Thr Leu Gln Ala Ser 
        435                 440                 445             


Glu Leu Ile Ser Arg Leu Gln Asp Asp Val Met Thr Tyr Gln Phe Glu 
    450                 455                 460                 


Arg Glu Arg Gly Gln Ser Ala Thr Gly Val Asp Ala Tyr Ile Lys Thr 
465                 470                 475                 480 


Tyr Gly Val Ser Glu Lys Glu Ala Ile Asp Glu Leu Lys Ile Met Ile 
                485                 490                 495     


Glu Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys Leu Lys Pro Arg Glu 
            500                 505                 510         


Val Ser Met Asp Leu Leu Ala Pro Ile Leu Asn Leu Ala Arg Met Ile 
        515                 520                 525             


Asp Val Val Tyr Arg Tyr Asp Asp Gly Phe Thr Phe Pro Gly Lys Thr 
    530                 535                 540                 


Met Lys Glu Tyr Ile Thr Leu Leu Phe Val Gly Ser Val Ser Met 
545                 550                 555                 


<210>  7
<211>  1677
<212>  DNA
<213>  Cichorium intybus

<400>  7
atggcagcag ttgaagccaa cggtaccctc caaacaaaca ccaaaaccac agagccagtg       60

cgtcctttag ccaacttccc tccttctgta tggggtgatc gattcttgtc attctctctt      120

gacactaagg aattggaagg atatgcaaaa gctatggagg agccaaaaga agaactgaga      180

aaactgattg tagatccaac aatggattca aataaaaaac taagtttgat ttattctgta      240

caccgtcttg gtttggcata tctgttcttg caagagattg aagcgcagct tgacaaacta      300

tttaaagagt ttaacttgca agattatgat gagtttgatc tatacacaac ttctattaac      360

tttcaagttt tcagacacct tggtcacaaa ctgccatgtg atgtgtttaa caaattcaag      420

gacagtagct tgggtacatt caaggaatct attaccaacg atgtgaaggg tatgttaggc      480

ttatatgaat ctgcacaatt gagattaaga ggagaaccca ttctcgatga agcttccgca      540

ttcactgaaa ctcaactcaa gagtgtagta aacactctcg aaggcaatct tgcaaaacag      600

gtgatgcaat cattgaggag accattccat caggggatgc caatggttga ggcaaggatg      660

tatttctcca actatgatga agaatgttcc acacatgagt cattacttaa gcttgcaaag      720

cttcatttca actatttgca gctacaacaa aaggaagaac ttcgcattgt ctcaaagtgg      780

tggaaggata tgaggttcca ggaaactact ccttacataa gggatagagt accagagatt      840

tacttatgga tattgggatt gtactttgag cctcgttact ccttggcacg aatcatcgcc      900

acaaaaataa cattgtttct ggtggtgcta gatgacacat atgatgcgta cgctaccatt      960

gaagagattc gacttctaac agatgccata aacaggtggg acattagtgc tatggagcag     1020

attccagaat acattcgacc attctacaaa attctcctag atgagtatgc tgaacttgag     1080

aagcaactag ctaaagaagg aagagcaaaa agtgttattg cttcaaaaga agcgttccag     1140

gacattgcaa gaggctacct tgaagaggcc gagtggacaa acagtggata tgtggcatca     1200

tttccagagt atatgaagaa cggtttaatt acttctgctt acaatgttat ttcaaaatct     1260

gctttagtgg gtatgggcga gatggttagt gaagatgcct tggcttggta tgaaagtcat     1320

ccaaagacac tgcaagcttc agagttaatt tcaagactcc aagacgatgt gatgacttac     1380

cagtttgagc gagaaagggg acaatcagcc accggcgttg attcttatat caagacctac     1440

ggcgtaacag aaaaggaagc gattgacgag ctaaacaaaa tgattgaaaa tgcatggaag     1500

gatataaatg agggctgcct taagccaaga gaagtctcaa tggatttgct tgccccaatt     1560

cttaatcttg cacgaatgat agacgtggta tacagatacg acgatgggtt cacttttcca     1620

ggaaagacca tgaaagagta tattactctg ttgtttgttg gttcttcacc catgtaa        1677


<210>  8
<211>  1677
<212>  DNA
<213>  Cichorium intybus

<400>  8
atggcagcag ttgaagccaa cggtaccctc caagcaaaca ccaaaaccac agagccagtg       60

cgtcctttag ccaacttccc tccttctgta tggggtgatc gattcttgtc attctctctt      120

gacactaagg aattggaagg atatgcaaaa gctatggagg agccaaaaga agaactgaga      180

aaactgattg tagatccaac aatggattca aaaaaaaaac taagtttgat ttattctgta      240

caccgtcttg gtttggcata tctgttcttg caagagattg aagcgcagct tgacaaacta      300

tttaaagagt ttaacttgca agattatgat gagtttgatc tatacacaac ttctattaac      360

tttcaagttt tcagacacct tggtcacaaa ctgccatgtg atgtgtttaa caaattcaag      420

gacagtagct tgggtacatt caaggaatct attaccaacg atgtgaaggg tatgttaggc      480

ttatatgaat ctgcacaatt gagattaaga ggagaaccca ttctcgatga agcttccgca      540

ttcactgaaa ctcaactcaa gagtgtagta aacactctcg aaggcaatct tgcaaaacag      600

gtgatgcaat cattgaggag accattccat caggggatgc caatggttga ggcaaggatg      660

tatttctcca actatgatga agaatgttcc acacatgagt cattacttaa gcttgcaaag      720

cttcatttca actatttgca gctacaacaa aaggaagaac ttcgcattgt ctcaaagtgg      780

tggaaggata tgaggttcca ggaaactact ccttacataa gggatagagt accagagatt      840

tacttatgga tattgggatt gtactttgag cctcgttact ccttggcacg aatcatcgcc      900

acaaaaataa cattgtttct ggtggtgcta gatgacacat atgatgcgta cgctaccatt      960

gaagagattc gacttctaac agatgccata aacaggtggg acattagtgc tatggagcag     1020

attccagaat acattcgacc attctacaaa attctcctag atgagtatgc tgaacttgag     1080

aagcaactag ctaaagaagg aagagcaaaa agtgttattg cttcaaaaga agcgttccag     1140

gacattgcaa gaggctacct tgaagaggcc gagtggacaa acagtggata tgtggcatca     1200

tttccagagt atatgaagaa cggtttaatt acttctgctt acaatgttat ttcaaaatct     1260

gctttagtgg gtatgggcga gatggttagt gaagatgcct tggcttggta tgaaagtcat     1320

ccaaagacac tgcaagcttc agagttaatt tcaagactcc aagacgatgt gatgacttac     1380

cagtttgagc gagaaagggg acaatcagcc accggcgttg attcttatat caagacctac     1440

ggcgtaacag aaaaggaagc gattgacgag ctaaacaaaa tgattgaaaa tgcatggaag     1500

gatataaatg agggctgcct taagccaaga gaagtctcaa tggatttgct tgccccaatt     1560

cttaatcttg cacgaatgat agacgtggta tacagatacg acgatgggtt cacttttcca     1620

ggaaagacca tgaaagagta tattactctg ttgtttgttg gttcttcacc catgtaa        1677


<210>  9
<211>  1680
<212>  DNA
<213>  Cichorium intybus

<400>  9
atggcagcag ttgaagccaa cggtaccttc caagcaaaca ccaaaaccac agagccggtg       60

cgtcctttag ccaacttccc tccttctgta tggggtgatc gattcttgtc attctctctt      120

gacactacgg aattggaagg atatgcaaaa gctatggagg agccaaaaga agaagtgaga      180

aaactgatcg tagatccaac aatggattca aataagaaac taagtttgat ttattctgta      240

caccgtcttg gtttgacata tctgttcttg caagagattg aagcgcagct tgacaaacta      300

tttaaagagt ttaacttgca agattatgat gagtttgatc tatacacaac ttctattaac      360

tttcaagttt tcagacacct tggtcacaaa ctgccatgtg atgtgtttaa caaattcaag      420

gacagtagct cgggtacatt caaggaatcc attaccaacg atgtgaaggg tatgttaggc      480

ttatatgaat ctgcacaatt gagattaaga ggagaaccca ttctcgatga agcttccgca      540

ttcactgaaa ctcaactcaa gagtgtagta aacactctcg aaggaaatct tgcaaaacag      600

gtgatgcaat cattgaggag accattccat caggggatgc caatggttga ggcaaggatg      660

tatttctcca actatgatga agaatgttcc acacacgagt cattacctaa gcttgcaaag      720

cttcatttca actatttgca gctacaacaa aaggaagaac ttcgcattgt ctcaaagtgg      780

tggaaggata tgaggttcca ggaaactact ccttacataa gggatagagt accagagatt      840

tacttatgga tattgggatt gtactttgag cctcgttact ccttggcacg aatcatcgcc      900

acaaaaatta cattgttcct ggtggttcta gatgacacat atgatgcata cgctaccatt      960

gaagagattc gacttctgac agatgccata aacaggtggg acatcagtgc tatggagcaa     1020

attccggaat atattcgacc attctacaaa attctcctag atgagtatgc tgaacttgag     1080

aagcaactag ctaaagaagg aagagcaaaa agtgtcattg cttcaaaaga agcgttccag     1140

gacattgcaa gaggatacct tgaagaggcc gagtggacaa acagtggata tgtggcatca     1200

tttccagagt atatgaagaa cggtttaatt acttctgctt acaatgttat ttcaaaatct     1260

gctttagtgg gtatgggcga gatggttggt gaagatgcct tggcttggta tgaaagtcat     1320

ccaaagacat tgcaagcttc agagttaatt tcaagactcc aagatgatgt gatgacttac     1380

cagtttgagc gagaaagggg acaatcagcc accggcgttg attcttatat caagacctat     1440

ggcgtaacag aaaaggaagc gattgacgag ctcaataaaa tgattgaaaa tgcatggaag     1500

gatataaatg agggctgcct taagccaaga gaagtctcaa tggatttgct tgccccaatt     1560

cttaatcttg cacgaatgat agatgtggta tacaggtacg acgatgggtt cacttttccg     1620

ggaaagacca tgaaagagta tattactctg ttgtttgttg gttcttcacc catgtaataa     1680


<210>  10
<211>  1680
<212>  DNA
<213>  Cichorium intybus

<400>  10
atggcagcag ttgaagccaa cggtaccttc caagcaaaca ccaaaaccac agagccggtg       60

cgtcctttag ccaacttccc tccttctgta tggggtgatc gattcttgtc attctctctt      120

gacactacgg aattggaagg atatgcaaaa gctatggagg agccaaaaga agaagtgaga      180

aaactgatcg tagatccaac aatggattca aataagaaac taagtttgat ttattctgta      240

caccgtcttg gtttgacata tctgttcttg caagagattg aagcgcagct tgacaaacta      300

tttaaagagt ttaacttgca agattatgat gagtttgatc tatacacaac ttctattaac      360

tttcaagttt tcagacacct tggtcacaaa ctgccatgtg atgtgtttaa caaattcaag      420

gacagtagct cgggtacatt caaggaatcc attaccaacg atgtgaaggg tatgttaggc      480

ttatatgaat ctgcacaatt gagattaaga ggagaaccca ttctcgatga agcttccgca      540

ttcactgaaa ctcaactcaa gagtgtagta aacactctcg aaggcaatct tgcaaaacag      600

gtgatgcaat cattgaggag accattccat caggggatgc caatggttga ggcaaggatg      660

tatttctcca actatgatga agaatgttcc acacacgagt cattacctaa gcttgcaaag      720

cttcatttca actatttgca gctacaacaa aaggaagaac ttcgcattgt ctcaaagtgg      780

tggaaggata tgaggttcca ggaaactact ccttacataa gggatagagt accagagatt      840

tacttatgga tattgggatt gtactttgag cctcgttact ccttggcacg aatcatcgcc      900

acaaaaatta cattgttcct cgtggttcta gatgacacat atgatgcata cgctaccatt      960

gaagagattc gacttctgac agatgccata aacaggtggg acatcagtgc tatggagcaa     1020

attccggaat atattcgacc attctacaaa attctcctag atgagtatgc tgaacttgag     1080

aagcaactag ctaaagaagg aagagcaaaa agtgttattg cttcaaaaga agcgttccag     1140

gacattgcaa gaggatacct tgaagaggcc gagtggacaa acagtggata tgtggcatca     1200

tttccagagt atatgaagaa cggtttaatt acttctgctt acaatgttat ttcaaaatct     1260

gctttagtgg gtatgggcga gatggttggt gaagatgcct tggcttggta tgaaagtcat     1320

ccaaagacat tgcaagcttc agagttaatt tcaagactcc aagatgatgt catgacttac     1380

cagtttgagc gagaaagggg acaatcagcc accggcgttg attcttatat caagacctat     1440

ggcgtaacag aaaaggaagc gattgacgag ctcaataaaa tgattgaaaa tgcatggaag     1500

gatataaatg agggctgcct taagccaaga gaagtctcaa tggatttgct tgccccaatt     1560

cttaatcttg cacgaatgat agatgtggta tacaggtacg acgatgggtt cacttttccg     1620

ggaaagacca tgaaagagta tattactctg ttgtttgttg gttcttcacc catgtaataa     1680


<210>  11
<211>  1680
<212>  DNA
<213>  Cichorium intybus

<400>  11
atggccgcag tcgaaaccaa tggtaccatc caagcgaaga ccaataccac cgctgagcca       60

gtgcgtcccc tagccaactt ccctccttct gtatggggtg atcgcttcct atcgttcacc      120

cttgacaatt cggagttgga aggatatgct agagcaatgg aagaaccaaa agaagatttg      180

agaagattga tcacagatgc aacaatggaa tccaataaga aactaagttt gatatattct      240

gtgcaccgtc ttggtttgac atatctattc ttgcaagaga ttgaagccca gctagacaaa      300

cttttcaaag agtttaattt gcaagattac attgaccttg atctttatac actttccatt      360

aactttcaag ttttccgaca ccttggttat aaactgcctt gtgacgtgtt taacaaaatc      420

aaggacagta actcgggtac attcaaggaa gacatttcca ccgatgtgaa gggaatgtta      480

ggcttatatg aatctgcaca actgagagta agaggagaat cgattctaga tgaagcctca      540

gcattcactg aagctaaact caagagtgta gtaaacactc tcgaaggtaa tcttgcaaaa      600

caggtgatgc aatctttgag aagaccgttc catcaaggga tgcccatggt cgaggcaagg      660

ctatatttct ccaactatga tgaagattgt tctacacacg agtcattact taagcttgca      720

aagctccatt tcaactattt agagctacaa caaaaggaag aacttcgcat tgtctcaaag      780

tggtggaagg atatgaggtt ccaagaaact actccttata taagggatag agtacccgag      840

atctacttat ggatattagg attgtacttt gagcctcgtt actccttggc acgaatcatc      900

gccacaaaaa ttacattgtt tttggtggtg ctagacgaca catatgatgc atatgctaca      960

attgaagaaa ttcgtctttt aactgatgcc ataaataggt gggacatgag tgctattgag     1020

caaatcccgg aatacattag accattttac aaaattctcc tggatgagta tgctgatctt     1080

gagaaacaac tagccataga aggaagagca aatagcgtta tagcttcaaa agaagcgttc     1140

caagacattg ctagaggtta ccttgaagag gctgagtgga caaacagtgg atatgtggca     1200

tcatttccag agtacatgaa gaacggttta attacttctg cttacaatgt tatttcaaaa     1260

tctgctttag tgggtatggg cgagatagtt agtgaaaatg cattagcttg gtacgaaagt     1320

catccaaaga ctctacaagc ttccgagtta atctcaagac tccaagatga tgtcatgact     1380

taccagtttg agcgtgaaag aggacaatca gccactggcg ttgatgcata tatcaagaca     1440

tatggcgtgt cagagaagga agctattgac gagctcaaga taatgattga aaatgcatgg     1500

aaagatataa acgagggatg tctcaagcca agagaagtct ccatggattt gttggcgcca     1560

attcttaacc ttgcaagaat gatagatgtt gtgtatagat atgatgacgg gttcactttt     1620

cccgggaaaa ccatgaaaga gtatattact cttttatttg ttggttctgt atccatgtaa     1680


<210>  12
<211>  1680
<212>  DNA
<213>  Cichorium intybus

<400>  12
atggccgcag tcgaaaccaa tggtaccatc caagcgaaga ccaataccac cgctgagcca       60

gtgcgtcccc tagccaactt ccctccttct gtatggggtg atcgcttcct atcgttcacc      120

cttgacaatt cggagttgga aggatatgct agagcaatgg aagaaccaaa agaagatttg      180

agaagattga tcacagatgc aacaatggaa tccaataaga aactaagttt gatatattct      240

gtgcaccgtc ttggtttgac atatctattc ttgcaagaga ttgaagccca gctagacaaa      300

cttttcaaag agttcaattt gcaagattac attgaccttg atctttatac actttccatt      360

aactttcaag ttttccgaca ccttggttat aaactgcctt gtgacgtgtt taacaaaatc      420

aaggtcagta actcgggtac attcaaggaa gacatttcca ccgatgtgaa gggaatgtta      480

ggcttatatg aatctgcaca actgagagta agaggagaat cgattctaga tgaagcctca      540

gcattcactg aagctaaact caagagtgta gtaaacactc tcgaaggtaa tcttgcaaaa      600

caggtgatgc aatctttgag aagaccgttc catcaaggga tgcccatggt cgaggcaagg      660

ctatatttct ccaactatga tgaagattgt tctacacacg agtcattact taagcttgca      720

aagctccatt tcaactattt agagctacaa caaaaggaag aacttcgcat tgtctcaaag      780

tggtggaagg atatgaggtt ccaagaaact actccttata taagggatag agtacccgag      840

atctacttat ggatattagg attgtacttt gagcctcgtt actccttggc acgaatcatc      900

gccacaaaaa ttacattgtt tttggtggtg ctagacgaca catatgatgc atatgctaca      960

attgaagaaa ttcgtctttt aactgatgcc ataaataggt gggacatgag tgctattgag     1020

caaatcccgg aatacattag accattttac aaaattctcc tggatgagta tgctgatctt     1080

gagaaacaac tagccataga aggaagagca aatagcgtta tagcttcaaa agaagcgttc     1140

caagacattg ctagaggtta ccttgaagag gctgagtgga caaacagtgg atatgtggca     1200

tcatttccag agtacatgaa gaacggttta attacttctg cttacaatgt tatttcaaaa     1260

tctgctttag tgggtatggg cgagatagtt agtgaaaatg cattagcttg gtacgaaagt     1320

catccaaaga ctctacaagc ttccgagtta atctcaagac tccaagatga tgtcatgact     1380

taccagtttg agcgtgaaag aggacaatca gccactggcg ttgatgcata tatcaagaca     1440

tatggcgtgt cagagaagga agctattgac gagctcaaga taatgattga aaatgcatgg     1500

aaagatataa acgagggatg tctcaagcca agagaagtct ccatggattt gttggcgcca     1560

attcttaacc ttgcaagaat gatagatgtt gtgtatagat atgatgacgg gttcactttt     1620

cccgggaaaa ccatgaaaga gtatattact cttttatttg ttggttctgt atccatgtaa     1680


<210>  13
<211>  583
<212>  PRT
<213>  Cichorium intybus

<400>  13

Met Ala Leu Val Arg Asn Asn Ser Ser Asn Gly Arg Glu Pro Val Leu 
1               5                   10                  15      


Ser Pro Arg Ser Leu Thr Ser Pro Arg Gly Leu Thr Ser Pro Arg Pro 
            20                  25                  30          


Leu Ser Val Gln Pro Thr Pro Glu Pro Val Arg Pro Leu Ala Asn Phe 
        35                  40                  45              


Pro Pro Ser Ile Trp Ala Asp Arg Phe Ile Ser Phe Ser Leu Asp Asn 
    50                  55                  60                  


Ser Gln Leu Glu Ala Tyr Ala Asn Ala Leu Glu Glu Pro Lys Glu Ala 
65                  70                  75                  80  


Val Lys Ser Leu Ile Thr Asp Thr Thr Ile Asp Ala Asn Thr Lys Leu 
                85                  90                  95      


Lys Leu Ile Tyr Ser Val His Arg Leu Gly Leu Ser Tyr Leu Tyr Pro 
            100                 105                 110         


Asp Glu Ile Asp Ala Glu Leu Asn Lys Leu Phe Glu Lys Ile Asp Leu 
        115                 120                 125             


Gln Tyr Tyr Glu Gln Val Asp Leu Tyr Thr Ile Ala Val Gln Phe Gln 
    130                 135                 140                 


Val Phe Arg His His Gly Tyr Lys Ile Ser Ser Asp Val Phe Lys Lys 
145                 150                 155                 160 


Phe Lys Asp Ser Thr Thr Gly Thr Phe Thr Asp Asp Val Thr Lys Asp 
                165                 170                 175     


Val Lys Gly Met Leu Ser Leu Tyr Glu Ser Ala His Leu Arg Leu His 
            180                 185                 190         


Gly Glu Asp Ile Leu Asp Glu Ala Leu Ala Phe Thr Glu Ala His Leu 
        195                 200                 205             


Lys Lys Ile Val Thr Thr Leu Glu Gly Asp Leu Ala Arg Gln Val Asn 
    210                 215                 220                 


Gln Val Leu Lys Arg Pro Phe His Thr Gly Met Pro Met Val Glu Ala 
225                 230                 235                 240 


Arg Leu Tyr Phe Ile Thr His Glu Glu Asp Phe Ser Ser His Glu Ser 
                245                 250                 255     


Val Val Lys Leu Ala Lys Val His Phe Asn Tyr Leu Gln Leu Gln Gln 
            260                 265                 270         


Lys Glu Glu Leu Arg Leu Val Ser Gln Trp Trp Lys Asp Met Gln Phe 
        275                 280                 285             


Gln Gln Ser Val Pro Tyr Ile Arg Asp Arg Val Pro Glu Ile Tyr Leu 
    290                 295                 300                 


Trp Ile Leu Gly Leu Tyr Phe Glu Pro Tyr Tyr Ser Arg Ala Arg Ile 
305                 310                 315                 320 


Ile Ala Thr Lys Ile Thr Leu Phe Leu Val Val Leu Asp Asp Thr Tyr 
                325                 330                 335     


Asp Ala Tyr Ala Thr Ile Asp Glu Ile Arg Ser Ile Thr Asp Ala Ile 
            340                 345                 350         


Asn Arg Trp Glu Ile Ser Ala Ile Asp Gln Leu Pro Glu Tyr Ile Lys 
        355                 360                 365             


Pro Phe Tyr Arg Ile Leu Leu Asn Glu Tyr Asp Asp Leu Glu Lys Glu 
    370                 375                 380                 


Tyr Ser Lys Asp Gly Arg Ala Phe Ser Val His Ala Ser Lys Gln Ala 
385                 390                 395                 400 


Phe Gln Glu Ile Ala Arg Gly Tyr Leu Glu Glu Ala Glu Trp Leu His 
                405                 410                 415     


Asn Gly Tyr Val Ala Thr Phe Pro Glu Tyr Met Lys Asn Gly Leu Ile 
            420                 425                 430         


Thr Ser Ala Tyr Asn Val Ile Ser Lys Ser Ala Leu Val Gly Met Gly 
        435                 440                 445             


Ala Ile Ala Asp Glu Glu Ala Leu Ala Trp Tyr Glu Thr His Pro Lys 
    450                 455                 460                 


Ile Leu Lys Ala Ser Glu Leu Ile Ser Arg Leu Gln Asp Asp Val Met 
465                 470                 475                 480 


Thr Phe Gln Phe Glu Arg Lys Arg Gly Gln Ser Ala Thr Gly Val Asp 
                485                 490                 495     


Ala Tyr Ile Lys Glu Tyr Asn Val Ser Glu Glu Val Ala Ile Gln Glu 
            500                 505                 510         


Leu Met Lys Met Ile Glu Asn Ala Trp Lys Asp Ile Asn Glu Gly Cys 
        515                 520                 525             


Leu Lys Pro Thr Glu Val Ser Val Ala Leu Leu Thr Pro Ile Leu Asn 
    530                 535                 540                 


Leu Ala Arg Met Ile Asp Val Val Tyr Lys Phe Asp Asp Gly Phe Thr 
545                 550                 555                 560 


Phe Pro Gly Lys Thr Leu Lys Asp Tyr Ile Thr Leu Leu Phe Val Ser 
                565                 570                 575     


Pro Pro Pro Ser Leu Glu Asn 
            580             


<210>  14
<211>  1752
<212>  DNA
<213>  Cichorium intybus

<400>  14
atggctctcg ttagaaacaa cagcagcaat ggtcgggagc cagttctcag ccccagaagt       60

ctcacaagtc ctcgaggctt aaccagcccc cgaccgttgt ctgtccaacc gactccagag      120

ccggttcgac ctttggccaa cttcccacct tcgatctggg ctgatcgctt catctcattc      180

tctcttgata actctcaatt ggaagcttat gcaaatgcac ttgaagaacc aaaagaagca      240

gtgaagagtt taataaccga cactaccatt gatgcaaaca caaaactgaa attgatttat      300

tcagtgcacc gtcttggttt gtcgtatctt tatccagatg agattgatgc cgaactcaac      360

aaactcttcg agaagattga cttacagtat tacgaacaag ttgatttgta cactattgca      420

gtacaatttc aagttttcag acaccatggt tataaaattt cttctgatgt gtttaaaaag      480

ttcaaggaca gtactacggg tacattcacg gatgatgtaa caaaagacgt gaagggtatg      540

ttgagtctat acgaatcagc acacttaagg ctacacggtg aagatatctt agatgaagct      600

ttagcattca ccgaagctca ccttaaaaaa atcgtaacca cacttgaagg agatcttgca      660

cgccaagtga accaagtctt aaaaagaccc ttccacactg gaatgccaat ggtagaggca      720

cggctatatt tcatcacaca cgaagaagat ttttcaagcc atgagtcggt tgtaaagcta      780

gctaaagtcc acttcaacta tttgcaacta caacaaaaag aagaactacg actcgtgtca      840

cagtggtgga aagatatgca attccaacaa tcggtccctt acataagaga tagagtaccg      900

gagatatacc tatggatttt ggggttatat ttcgagccgt attactctcg ggcacgtatc      960

atagccacta aaatcacgtt gttcttggtg gttttagatg atacgtatga cgcgtatgct     1020

acaattgacg agatccgatc gatcacagat gcgattaata ggtgggaaat tagcgcgatc     1080

gaccaacttc ctgaatatat caaaccgttc tacagaattc tcctcaacga atacgatgac     1140

ctcgagaaag aatactccaa ggacggaaga gcgttcagtg tccacgcttc aaaacaagcg     1200

tttcaagaaa tcgcgagagg gtatcttgaa gaggcggagt ggttacacaa cggttatgtg     1260

gcaacatttc ccgagtatat gaagaatggt ttgattactt cggcttataa tgtcatttca     1320

aaatccgcat tggtgggaat gggtgcgatt gcagatgaag aggctcttgc ttggtatgaa     1380

acacatccga aaattttgaa agcttcagag ttgatttcaa ggctccaaga cgatgttatg     1440

actttccagt ttgagagaaa acgaggacaa tcagcaactg gtgtggatgc ttatatcaag     1500

gaatacaatg tatccgaaga agtggcgatc caagagctca tgaagatgat tgaaaacgca     1560

tggaaagata taaatgaggg atgcttgaag cctacagagg tctcggtggc tttactaact     1620

cctattttga atctcgcgag aatgatagat gtcgtataca aatttgatga tgggttcacc     1680

tttcccggga aaaccctaaa agactatatt acccttttgt tcgttagtcc tccacctagt     1740

ctcgaaaatt ga                                                         1752


<210>  15
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal sequence of GAS-short protein

<400>  15

Met Ala Ala Val Glu Ala Asn Gly Thr Phe Gln Ala Asn Thr Lys Thr 
1               5                   10                  15      


Thr Glu Pro Val Arg Pro Leu Ala Asn Phe Pro Pro Ser Val Trp Gly 
            20                  25                  30          


Asp Arg Phe Leu Ser Phe Ser Leu 
        35                  40  


<210>  16
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal sequence of GAS-long protein

<400>  16

Met Ala Leu Val Arg Asn Asn Ser Ser Asn Gly Arg Glu Pro Val Leu 
1               5                   10                  15      


Ser Pro Arg Ser Leu Thr Ser Pro Arg Gly Leu Thr Ser Pro Arg Pro 
            20                  25                  30          


Leu Ser Val Gln Pro Thr Pro Glu 
        35                  40  


<210>  17
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting GAS-S1 and GAS-S2

<400>  17
ggtactctat cccttatgta gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  18
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting GAS-S3

<400>  18
ggtactctat cccttatata gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  19
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting GAS-L1

<400>  19
ggtactctat ctcttatgta gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  20
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette encoding sgRNA targeting GAS-S1 and GAS-S2

<400>  20
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg ggtactctat cccttatgta gttttagagc tagaaatagc      120

aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt      180

ttt                                                                    183


<210>  21
<211>  608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette comprising three sgRNAs targeting GAS-S1, 
       GAS-S2, GAS-S3 and GAS-L1

<400>  21
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg aacaaagcac cagtggtcta gtggtagaat agtaccctgc      120

cacggtacag acccgggttc gattcccggc tggtgcaggt actctatccc ttatgtagtt      180

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      240

accgagtcgg tgcaacaaag caccagtggt ctagtggtag aatagtaccc tgccacggta      300

cagacccggg ttcgattccc ggctggtgca ggtactctat cccttatata gttttagagc      360

tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt      420

cggtgcaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg gtacagaccc      480

gggttcgatt cccggctggt gcaggtactc tatctcttat gtagttttag agctagaaat      540

agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      600

tttttttt                                                               608


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of GAS-S1and GAS-S2

<400>  22
ggtactctat cccttatgta agg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of GAS-S3

<400>  23
ggtactctat cccttatata agg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of GAS-L1

<400>  24
ggtactctat ctcttatgta agg                                               23


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-1)

<400>  25
ggtactctat cccttattaa gg                                                22


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-4)

<400>  26
ggtactctat cctgtaagg                                                    19


<210>  27
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-7)

<400>  27
ggtactctat gtaagg                                                       16


<210>  28
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-9)

<400>  28
ggtactctat aagg                                                         14


<210>  29
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-12)

<400>  29
ggtacgtaag g                                                            11


<210>  30
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-14)

<400>  30
ggtgtaagg                                                                9


<210>  31
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 (-19)

<400>  31
taagg                                                                    5


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (+1)

<400>  32
ggtactctat cccttattgt aagg                                              24


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (-4)

<400>  33
ggtactctat cccgtaagg                                                    19


<210>  34
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (-7)

<400>  34
ggtactctat gtaagg                                                       16


<210>  35
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (-10)

<400>  35
ggtactctat agg                                                          13


<210>  36
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (-13)

<400>  36
ggttgtaagg                                                              10


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 (-2)

<400>  37
ggtactctat cccttataag g                                                 21


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 (-4)

<400>  38
ggtactctat cccataagg                                                    19


<210>  39
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 (-7)

<400>  39
ggtactctat ataagg                                                       16


<210>  40
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 (-9)

<400>  40
ggtactctat aagg                                                         14


<210>  41
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 (-16)

<400>  41
ggtaagg                                                                  7


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L1 (-1)

<400>  42
ggtactctat ctcttagtaa gg                                                22


<210>  43
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L1 (-5)

<400>  43
ggtactctat ctgtaagg                                                     18


<210>  44
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L1 (-6)

<400>  44
ggtactctat tgtaagg                                                      17


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 F primer

<400>  45
tgtaaaccgt tgggctcgtc                                                   20


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 F primer

<400>  46
ctattaatcg gctttggtca ac                                                22


<210>  47
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 F primer

<400>  47
gttatttcag ttccccaagt gt                                                22


<210>  48
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L F primer

<400>  48
cgtacgtaga gtgtccaa                                                     18


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1 R primer

<400>  49
gagtttatag cacatgcgt                                                    19


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S2 R primer

<400>  50
ccatatggac tcgctcaaac                                                   20


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 R primer

<400>  51
gtgtttttgt cctatccgtc t                                                 21


<210>  52
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L R primer

<400>  52
cccaaaagaa gcccaatcc                                                    19


<210>  53
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1/S2 nF primer

<400>  53
actctttccc tacacgacgc tcttccgatc ttatgaggtt ccaggaaact a                51


<210>  54
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 nF primer

<400>  54
actctttccc tacacgacgc tcttccgatc ttatgaggtt ccaagaaact a                51


<210>  55
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L nF primer

<400>  55
actctttccc tacacgacgc tcttccgatc ttatgcaatt ccaacaatcc g                51


<210>  56
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S1/S2 nR primer

<400>  56
tggagttcag acgtgtgctc ttccgatcta ggctcaaagt acaatccc                    48


<210>  57
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-S3 nR primer

<400>  57
tggagttcag acgtgtgctc ttccgatcta ggctcaaagt acaatcct                    48


<210>  58
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAS-L nR primer

<400>  58
tggagttcag acgtgtgctc ttccgatcta cggctcgaaa tataacccc                   49


<210>  59
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA

<400>  59
aacaaagcac cagtggtcta gtggtagaat agtaccctgc cacggtacag acccgggttc       60

gattcccggc tggtgca                                                      77


<210>  60
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  arabidopsis U6 promoter

<400>  60
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg                                                   80


<210>  61
<211>  1368
<212>  PRT
<213>  artificial sequence

<220>
<223>  Cas9

<400>  61

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  62
<211>  4104
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding Cas9

<400>  62
atggataaaa aatatagcat tggtctggat attggtacca atagcgttgg ttgggcagtt       60

attaccgatg aatataaagt tccgagcaaa aaatttaaag ttctgggtaa taccgatcgt      120

catagcatta aaaaaaatct gattggtgca ctgctgtttg atagcggtga aaccgcagaa      180

gcaacccgtc tgaaacgtac cgcacgtcgt cgttataccc gtcgtaaaaa tcgtatttgt      240

tatctgcagg aaatttttag caatgaaatg gcaaaagttg atgatagctt ttttcatcgt      300

ctggaagaaa gctttctggt tgaagaagat aaaaaacatg aacgtcatcc gatttttggt      360

aatattgttg atgaagttgc atatcatgaa aaatatccga ccatttatca tctgcgtaaa      420

aaactggttg atagcaccga taaagcagat ctgcgtctga tttatctggc actggcacat      480

atgattaaat ttcgtggtca ttttctgatt gaaggtgatc tgaatccgga taatagcgat      540

gttgataaac tgtttattca gctggttcag acctataatc agctgtttga agaaaatccg      600

attaatgcaa gcggtgttga tgcaaaagca attctgagcg cacgtctgag caaaagccgt      660

cgtctggaaa atctgattgc acagctgccg ggtgaaaaaa aaaatggtct gtttggtaat      720

ctgattgcac tgagcctggg tctgaccccg aattttaaaa gcaattttga tctggcagaa      780

gatgcaaaac tgcagctgag caaagatacc tatgatgatg atctggataa tctgctggca      840

cagattggtg atcagtatgc agatctgttt ctggcagcaa aaaatctgag cgatgcaatt      900

ctgctgagcg atattctgcg tgttaatacc gaaattacca aagcaccgct gagcgcaagc      960

atgattaaac gttatgatga acatcatcag gatctgaccc tgctgaaagc actggttcgt     1020

cagcagctgc cggaaaaata taaagaaatt ttttttgatc agagcaaaaa tggttatgca     1080

ggttatattg atggtggtgc aagccaggaa gaattttata aatttattaa accgattctg     1140

gaaaaaatgg atggtaccga agaactgctg gttaaactga atcgtgaaga tctgctgcgt     1200

aaacagcgta cctttgataa tggtagcatt ccgcatcaga ttcatctggg tgaactgcat     1260

gcaattctgc gtcgtcagga agatttttat ccgtttctga aagataatcg tgaaaaaatt     1320

gaaaaaattc tgacctttcg tattccgtat tatgttggtc cgctggcacg tggtaatagc     1380

cgttttgcat ggatgacccg taaaagcgaa gaaaccatta ccccgtggaa ttttgaagaa     1440

gttgttgata aaggtgcaag cgcacagagc tttattgaac gtatgaccaa ttttgataaa     1500

aatctgccga atgaaaaagt tctgccgaaa catagcctgc tgtatgaata ttttaccgtt     1560

tataatgaac tgaccaaagt taaatatgtt accgaaggta tgcgtaaacc ggcatttctg     1620

agcggtgaac agaaaaaagc aattgttgat ctgctgttta aaaccaatcg taaagttacc     1680

gttaaacagc tgaaagaaga ttattttaaa aaaattgaat gttttgatag cgttgaaatt     1740

agcggtgttg aagatcgttt taatgcaagc ctgggtacct atcatgatct gctgaaaatt     1800

attaaagata aagattttct ggataatgaa gaaaatgaag atattctgga agatattgtt     1860

ctgaccctga ccctgtttga agatcgtgaa atgattgaag aacgtctgaa aacctatgca     1920

catctgtttg atgataaagt tatgaaacag ctgaaacgtc gtcgttatac cggttggggt     1980

cgtctgagcc gtaaactgat taatggtatt cgtgataaac agagcggtaa aaccattctg     2040

gattttctga aaagcgatgg ttttgcaaat cgtaatttta tgcagctgat tcatgatgat     2100

agcctgacct ttaaagaaga tattcagaaa gcacaggtta gcggtcaggg tgatagcctg     2160

catgaacata ttgcaaatct ggcaggtagc ccggcaatta aaaaaggtat tctgcagacc     2220

gttaaagttg ttgatgaact ggttaaagtt atgggtcgtc ataaaccgga aaatattgtt     2280

attgaaatgg cacgtgaaaa tcagaccacc cagaaaggtc agaaaaatag ccgtgaacgt     2340

atgaaacgta ttgaagaagg tattaaagaa ctgggtagcc agattctgaa agaacatccg     2400

gttgaaaata cccagctgca gaatgaaaaa ctgtatctgt attatctgca gaatggtcgt     2460

gatatgtatg ttgatcagga actggatatt aatcgtctga gcgattatga tgttgatcat     2520

attgttccgc agagctttct gaaagatgat agcattgata ataaagttct gacccgtagc     2580

gataaaaatc gtggtaaaag cgataatgtt ccgagcgaag aagttgttaa aaaaatgaaa     2640

aattattggc gtcagctgct gaatgcaaaa ctgattaccc agcgtaaatt tgataatctg     2700

accaaagcag aacgtggtgg tctgagcgaa ctggataaag caggttttat taaacgtcag     2760

ctggttgaaa cccgtcagat taccaaacat gttgcacaga ttctggatag ccgtatgaat     2820

accaaatatg atgaaaatga taaactgatt cgtgaagtta aagttattac cctgaaaagc     2880

aaactggtta gcgattttcg taaagatttt cagttttata aagttcgtga aattaataat     2940

tatcatcatg cacatgatgc atatctgaat gcagttgttg gtaccgcact gattaaaaaa     3000

tatccgaaac tggaaagcga atttgtttat ggtgattata aagtttatga tgttcgtaaa     3060

atgattgcaa aaagcgaaca ggaaattggt aaagcaaccg caaaatattt tttttatagc     3120

aatattatga atttttttaa aaccgaaatt accctggcaa atggtgaaat tcgtaaacgt     3180

ccgctgattg aaaccaatgg tgaaaccggt gaaattgttt gggataaagg tcgtgatttt     3240

gcaaccgttc gtaaagttct gagcatgccg caggttaata ttgttaaaaa aaccgaagtt     3300

cagaccggtg gttttagcaa agaaagcatt ctgccgaaac gtaatagcga taaactgatt     3360

gcacgtaaaa aagattggga tccgaaaaaa tatggtggtt ttgatagccc gaccgttgca     3420

tatagcgttc tggttgttgc aaaagttgaa aaaggtaaaa gcaaaaaact gaaaagcgtt     3480

aaagaactgc tgggtattac cattatggaa cgtagcagct ttgaaaaaaa tccgattgat     3540

tttctggaag caaaaggtta taaagaagtt aaaaaagatc tgattattaa actgccgaaa     3600

tatagcctgt ttgaactgga aaatggtcgt aaacgtatgc tggcaagcgc aggtgaactg     3660

cagaaaggta atgaactggc actgccgagc aaatatgtta attttctgta tctggcaagc     3720

cattatgaaa aactgaaagg tagcccggaa gataatgaac agaaacagct gtttgttgaa     3780

cagcataaac attatctgga tgaaattatt gaacagatta gcgaatttag caaacgtgtt     3840

attctggcag atgcaaatct ggataaagtt ctgagcgcat ataataaaca tcgtgataaa     3900

ccgattcgtg aacaggcaga aaatattatt catctgttta ccctgaccaa tctgggtgca     3960

ccggcagcat ttaaatattt tgataccacc attgatcgta aacgttatac cagcaccaaa     4020

gaagttctgg atgcaaccct gattcatcag agcattaccg gtctgtatga aacccgtatt     4080

gatctgagcc agctgggtgg tgat                                            4104


<210>  63
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA scaffold

<400>  63
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt                                                   80


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA scaffold

<400>  64
gttttagagc tgtgttgttt cg                                                22


<210>  65
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  65
cgaaacagca tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc       60

gagtcggtgc ttt                                                          73


<210>  66
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  arabidopsis ubiquitin promoter

<400>  66
aaaaattacg gatatgaata taggcatatc cgtatccgaa ttatccgttt gacagctagc       60

aacgattgta caattgcttc tttaaaaaag gaagaaagaa agaaagaaaa gaatcaacat      120

cagcgttaac aaacggcccc gttacggccc aaacggtcat atagagtaac ggcgttaagc      180

gttgaaagac tcctatcgaa atacgtaacc gcaaacgtgt catagtcaga tcccctcttc      240

cttcaccgcc tcaaacacaa aaataatctt ctacagccta tatatacaac ccccccttct      300

atctctcctt tctcacaatt catcatcttt ctttctctac ccccaatttt aagaaatcct      360

ctcttctcct cttcattttc aaggtaaatc tctctctctc tctctctctc tgttattcct      420

tgttttaatt aggtatgtat tattgctagt ttgttaatct gcttatctta tgtatgcctt      480

atgtgaatat ctttatcttg ttcatctcat ccgtttagaa gctataaatt tgttgatttg      540

actgtgtatc tacacgtggt tatgtttata tctaatcaga tatgaatttc ttcatattgt      600

tgcgtttgtg tgtaccaatc cgaaatcgtt gatttttttc atttaatcgt gtagctaatt      660

gtacgtatac atatggatct acgtatcaat tgttcatctg tttgtgtttg tatgtataca      720

gatctgaaaa catcacttct ctcatctgat tgtgttgtta catacataga tatagatctg      780

ttatatcatt ttttttatta attgtgtata tatatatgtg catagatctg gattacatga      840

ttgtgattat ttacatgatt ttgttattta cgtatgtata tatgtagatc tggacttttt      900

ggagttgttg acttgattgt atttgtgtgt gtatatgtgt gttctgatct tgatatgtta      960

tgtatgtgca gc                                                          972


<210>  67
<211>  220
<212>  DNA
<213>  Cichorium intybus

<400>  67
gtggtggaag gatatgaggt tccaggaaac tactccttac ataagggata gagtaccaga       60

gatttactta tggatattgg gattgtactt tgagcctcgt tactccttgg cacgaatcat      120

cgccacaaaa ataacattgt ttctggtggt gctagatgac acatatgatg cgtacgctac      180

cattgaagag attcgacttc taacagatgc cataaacagg                            220


<210>  68
<211>  220
<212>  DNA
<213>  Cichorium intybus

<400>  68
gtggtggaag gatatgaggt tccaggaaac tactccttac ataagggata gagtaccaga       60

gatttactta tggatattgg gattgtactt tgagcctcgt tactccttgg cacgaatcat      120

cgccacaaaa attacattgt tcctggtggt tctagatgac acatatgatg catacgctac      180

cattgaagag attcgacttc tgacagatgc cataaacagg                            220


<210>  69
<211>  220
<212>  DNA
<213>  Cichorium intybus

<400>  69
gtggtggaag gatatgaggt tccaagaaac tactccttat ataagggata gagtacccga       60

gatctactta tggatattag gattgtactt tgagcctcgt tactccttgg cacgaatcat      120

cgccacaaaa attacattgt ttttggtggt gctagacgac acatatgatg catatgctac      180

aattgaagaa attcgtcttt taactgatgc cataaatagg                            220


<210>  70
<211>  220
<212>  DNA
<213>  Cichorium intybus

<400>  70
gtggtggaaa gatatgcaat tccaacaatc ggtcccttac ataagagata gagtaccgga       60

gatataccta tggattttgg ggttatattt cgagccgtat tactctcggg cacgtatcat      120

agccactaaa atcacgttgt tcttggtggt tttggacgat acatatgacg cgtatgctac      180

aattgacgag atccgatcga tcacagatgc gattaatagg                            220


<210>  71
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GAS-S3 (-11)

<400>  71
ggtactataa gg                                                           12


