                         SEQUENCE LISTING

<110>  Navigo Proteins GmbH
 
<120>  BINDING PROTEINS FOR THE ENZYME ACID ALPHA-GLUCOSIDASE AND USES 
       THEREOF

<130>  NP43 WO

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (monomer)

<400>  1

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys 
    50                  55              


<210>  2
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein

<400>  2

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Ala Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Ser Gln Ala Pro Lys 
    50                  55              


<210>  3
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (with C-terminal Cys)

<400>  3

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys Cys 
    50                  55                  


<210>  4
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (C-terminal Cys)

<400>  4

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Ala Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Ser Gln Ala Pro Lys Cys 
    50                  55                  


<210>  5
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (N-cap and C-terminal Cys)

<400>  5

Val Asp Ala Lys Phe Asp Ile Ala Ala Lys Phe Asp Met Lys Gln Ala 
1               5                   10                  15      


Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln 
            20                  25                  30          


Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro Ser Val Ser Asp 
        35                  40                  45              


Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met Gln Ala Pro Lys 
    50                  55                  60                  


Cys 
65  


<210>  6
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (N-cap and C-terminal Cys)

<400>  6

Val Asp Ala Lys Phe Asp Ile Ala Ala Lys Phe Asp Met Lys Gln Ala 
1               5                   10                  15      


Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln 
            20                  25                  30          


Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro Ser Val Ser Asp 
        35                  40                  45              


Leu Val Leu Ala Gln Ala Gln Lys Leu Asn Gln Ser Gln Ala Pro Lys 
    50                  55                  60                  


Cys 
65  


<210>  7
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (dimer)

<400>  7

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp 
    50                  55                  60                  


Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met 
            100                 105                 110         


Gln Ala Pro Lys 
        115     


<210>  8
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein (dimer with C-terminal Cys)

<400>  8

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp 
    50                  55                  60                  


Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met 
            100                 105                 110         


Gln Ala Pro Lys Cys 
        115         


<210>  9
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein GAA binding protein

<400>  9

Ile Ala Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala Asp Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Val Leu Gly Glu Ala 
        35                  40                  45              


Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp 
    50                  55                  60                  


Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala 
        115                 120                 125             


Asp Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Arg Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Val 
145                 150                 155                 160 


Leu Gly Glu Ala Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys 
                165                 170                 


<210>  10
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  non Fc binding protein; scaffold protein

<400>  10

Ile Ala Ala Lys Phe Asp Glu Ala Gln Ser Ala Ala Asp Ser Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Leu Glu Val Leu Gly Glu Ala 
        35                  40                  45              


Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys 
    50                  55              


<210>  11
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  non-Fc binding protein

<400>  11

Ile Ala Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala Asp Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Val Leu Gly Glu Ala 
        35                  40                  45              


Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys 
    50                  55              


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptag

<400>  12

Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flag-tag

<400>  13

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  14
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  204870 cor1-PAdelFc2-PAdelFc2

<400>  14

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp 
    50                  55                  60                  


Glu Ala Gln Gln Ala Ala Asp Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Arg Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Leu Glu Val Leu Gly Glu Ala Gln Lys Leu Asn Asp Ser 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala 
        115                 120                 125             


Asp Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Arg Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Val 
145                 150                 155                 160 


Leu Gly Glu Ala Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys 
                165                 170                 


<210>  15
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  core1 dimer - PAdelFc2 dimer

<400>  15

Ile Ala Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile 
1               5                   10                  15      


Leu Glu Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln 
            20                  25                  30          


Ser Leu Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala 
        35                  40                  45              


Gln Lys Leu Asn Gln Met Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp 
    50                  55                  60                  


Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala 
        115                 120                 125             


Asp Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Arg Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Val 
145                 150                 155                 160 


Leu Gly Glu Ala Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys Ile Ala 
                165                 170                 175     


Ala Lys Phe Asp Glu Ala Gln Gln Ala Ala Asp Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu 
        195                 200                 205             


Arg Asp Asp Pro Ser Val Ser Leu Glu Val Leu Gly Glu Ala Gln Lys 
    210                 215                 220                 


Leu Asn Asp Ser Gln Ala Pro Lys 
225                 230         


<210>  16
<211>  56
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein

<400>  16

Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu 
1               5                   10                  15      


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu 
            20                  25                  30          


Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys 
        35                  40                  45              


Leu Asn Gln Met Gln Ala Pro Lys 
    50                  55      


<210>  17
<211>  56
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein

<400>  17

Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu 
1               5                   10                  15      


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu 
            20                  25                  30          


Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Ala Gln Ala Gln Lys 
        35                  40                  45              


Leu Asn Gln Ser Gln Ala Pro Lys 
    50                  55      


<210>  18
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein


<220>
<221>  Xaa
<222>  (38)..(38)
<223>  Xaa may be selected from any amino acid

<220>
<221>  Xaa
<222>  (46)..(46)
<223>  Xaa may be selected from any amino acid

<400>  18

Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Asp Leu Val Leu Xaa Gln Ala Gln Lys Leu Asn Gln Xaa 
        35                  40                  45      


<210>  19
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising GAA binding protein

<400>  19

Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu 
1               5                   10                  15      


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu 
            20                  25                  30          


Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Leu Gln Ala Gln Lys 
        35                  40                  45              


Leu Asn Gln Met Gln Ala Pro Lys Ala Gln His Asp Lys Asp Gln Gln 
    50                  55                  60                  


Ala Ala Asp Lys Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln 
65                  70                  75                  80  


Arg Asn Lys Phe Arg Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Ala 
                85                  90                  95      


Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
            100                 105                 110         


Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Asp Lys Glu Ile 
        115                 120                 125             


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Lys Phe Arg Gln 
    130                 135                 140                 


Ser Leu Arg Asp Asp Pro Ser Val Ser Ala Glu Ile Leu Ala Glu Ala 
145                 150                 155                 160 


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Cys 
                165                 170     


<210>  20
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising GAA binding protein

<400>  20

Ala Lys Phe Asp Met Lys Gln Ala Trp Ala Asp His Phe Ile Leu Glu 
1               5                   10                  15      


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu 
            20                  25                  30          


Ser Asp Asp Pro Ser Val Ser Asp Leu Val Leu Ala Gln Ala Gln Lys 
        35                  40                  45              


Leu Asn Gln Ser Gln Ala Pro Lys Ala Gln His Asp Lys Asp Gln Gln 
    50                  55                  60                  


Ala Ala Asp Lys Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln 
65                  70                  75                  80  


Arg Asn Lys Phe Arg Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Ala 
                85                  90                  95      


Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
            100                 105                 110         


Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Asp Lys Glu Ile 
        115                 120                 125             


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Lys Phe Arg Gln 
    130                 135                 140                 


Ser Leu Arg Asp Asp Pro Ser Val Ser Ala Glu Ile Leu Ala Glu Ala 
145                 150                 155                 160 


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Cys 
                165                 170     


<210>  21
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  non Ig binding protein

<400>  21

Ile Ala Ala Gln His Asp Lys Ile Gln Gln Ala Ala Asp Lys Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Lys Phe Arg Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Ala Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  22
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein

<400>  22

Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Asp Leu Val Leu Leu Gln Ala Gln Lys Leu Asn Gln Met 
        35                  40                  45      


<210>  23
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAA binding protein

<400>  23

Gln Ala Trp Ala Asp His Phe Ile Leu Glu Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Arg Gln Ser Leu Ser Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Asp Leu Val Leu Ala Gln Ala Gln Lys Leu Asn Gln Ser 
        35                  40                  45      


