                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals SA
 
<120>  S. AUREUS ANTIGENS AND COMPOSITIONS THEREOF

<130>  VB66801 WO

<140>  
<141>  

<150>  EP19218329.1
<151>  2019-12-19

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  301
<212>  PRT
<213>  Artificial

<220>
<223>  lukD (Delta)sp (signal peptide removed)

<400>  1

Ala Gln His Ile Thr Pro Val Ser Glu Lys Lys Val Asp Asp Lys Ile 
1               5                   10                  15      


Thr Leu Tyr Lys Thr Thr Ala Thr Ser Asp Asn Asp Lys Leu Asn Ile 
            20                  25                  30          


Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp Lys 
        35                  40                  45              


Asp Thr Leu Val Leu Lys Ala Ala Gly Asn Ile Asn Ser Gly Tyr Lys 
    50                  55                  60                  


Lys Pro Asn Pro Lys Asp Tyr Asn Tyr Ser Gln Phe Tyr Trp Gly Gly 
65                  70                  75                  80  


Lys Tyr Asn Val Ser Val Ser Ser Glu Ser Asn Asp Ala Val Asn Val 
                85                  90                  95      


Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Phe Gln Val Gln Gln 
            100                 105                 110         


Thr Leu Gly Tyr Ser Tyr Gly Gly Asp Ile Asn Ile Ser Asn Gly Leu 
        115                 120                 125             


Ser Gly Gly Leu Asn Gly Ser Lys Ser Phe Ser Glu Thr Ile Asn Tyr 
    130                 135                 140                 


Lys Gln Glu Ser Tyr Arg Thr Thr Ile Asp Arg Lys Thr Asn His Lys 
145                 150                 155                 160 


Ser Ile Gly Trp Gly Val Glu Ala His Lys Ile Met Asn Asn Gly Trp 
                165                 170                 175     


Gly Pro Tyr Gly Arg Asp Ser Tyr Asp Pro Thr Tyr Gly Asn Glu Leu 
            180                 185                 190         


Phe Leu Gly Gly Arg Gln Ser Ser Ser Asn Ala Gly Gln Asn Phe Leu 
        195                 200                 205             


Pro Thr His Gln Met Pro Leu Leu Ala Arg Gly Asn Phe Asn Pro Glu 
    210                 215                 220                 


Phe Ile Ser Val Leu Ser His Lys Gln Asn Asp Thr Lys Lys Ser Lys 
225                 230                 235                 240 


Ile Lys Val Thr Tyr Gln Arg Glu Met Asp Arg Tyr Thr Asn Gln Trp 
                245                 250                 255     


Asn Arg Leu His Trp Val Gly Asn Asn Tyr Lys Asn Gln Asn Thr Val 
            260                 265                 270         


Thr Phe Thr Ser Thr Tyr Glu Val Asp Trp Gln Asn His Thr Val Lys 
        275                 280                 285             


Leu Ile Gly Thr Asp Ser Lys Glu Thr Asn Pro Gly Val 
    290                 295                 300     


<210>  2
<211>  261
<212>  PRT
<213>  Artificial

<220>
<223>  lukD (Delta)sp(Delta)stem (signal peptide and stem removed)

<400>  2

Ala Gln His Ile Thr Pro Val Ser Glu Lys Lys Val Asp Asp Lys Ile 
1               5                   10                  15      


Thr Leu Tyr Lys Thr Thr Ala Thr Ser Asp Asn Asp Lys Leu Asn Ile 
            20                  25                  30          


Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp Lys 
        35                  40                  45              


Asp Thr Leu Val Leu Lys Ala Ala Gly Asn Ile Asn Ser Gly Tyr Lys 
    50                  55                  60                  


Lys Pro Asn Pro Lys Asp Tyr Asn Tyr Ser Gln Phe Tyr Trp Gly Gly 
65                  70                  75                  80  


Lys Tyr Asn Val Ser Val Ser Ser Glu Ser Asn Asp Ala Val Asn Val 
                85                  90                  95      


Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Ser Tyr Arg Thr Thr 
            100                 105                 110         


Ile Asp Arg Lys Thr Asn His Lys Ser Ile Gly Trp Gly Val Glu Ala 
        115                 120                 125             


His Lys Ile Met Asn Asn Gly Trp Gly Pro Tyr Gly Arg Asp Ser Tyr 
    130                 135                 140                 


Asp Pro Thr Tyr Gly Asn Glu Leu Phe Leu Gly Gly Arg Gln Ser Ser 
145                 150                 155                 160 


Ser Asn Ala Gly Gln Asn Phe Leu Pro Thr His Gln Met Pro Leu Leu 
                165                 170                 175     


Ala Arg Gly Asn Phe Asn Pro Glu Phe Ile Ser Val Leu Ser His Lys 
            180                 185                 190         


Gln Asn Asp Thr Lys Lys Ser Lys Ile Lys Val Thr Tyr Gln Arg Glu 
        195                 200                 205             


Met Asp Arg Tyr Thr Asn Gln Trp Asn Arg Leu His Trp Val Gly Asn 
    210                 215                 220                 


Asn Tyr Lys Asn Gln Asn Thr Val Thr Phe Thr Ser Thr Tyr Glu Val 
225                 230                 235                 240 


Asp Trp Gln Asn His Thr Val Lys Leu Ile Gly Thr Asp Ser Lys Glu 
                245                 250                 255     


Thr Asn Pro Gly Val 
            260     


<210>  3
<211>  265
<212>  PRT
<213>  Artificial

<220>
<223>  lukD Delta)sp(Delta)stem-PSGS (signal peptide removed, stem 
       replaced with PSGS-linker)

<400>  3

Ala Gln His Ile Thr Pro Val Ser Glu Lys Lys Val Asp Asp Lys Ile 
1               5                   10                  15      


Thr Leu Tyr Lys Thr Thr Ala Thr Ser Asp Asn Asp Lys Leu Asn Ile 
            20                  25                  30          


Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp Lys 
        35                  40                  45              


Asp Thr Leu Val Leu Lys Ala Ala Gly Asn Ile Asn Ser Gly Tyr Lys 
    50                  55                  60                  


Lys Pro Asn Pro Lys Asp Tyr Asn Tyr Ser Gln Phe Tyr Trp Gly Gly 
65                  70                  75                  80  


Lys Tyr Asn Val Ser Val Ser Ser Glu Ser Asn Asp Ala Val Asn Val 
                85                  90                  95      


Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Pro Ser Gly Ser Glu Ser 
            100                 105                 110         


Tyr Arg Thr Thr Ile Asp Arg Lys Thr Asn His Lys Ser Ile Gly Trp 
        115                 120                 125             


Gly Val Glu Ala His Lys Ile Met Asn Asn Gly Trp Gly Pro Tyr Gly 
    130                 135                 140                 


Arg Asp Ser Tyr Asp Pro Thr Tyr Gly Asn Glu Leu Phe Leu Gly Gly 
145                 150                 155                 160 


Arg Gln Ser Ser Ser Asn Ala Gly Gln Asn Phe Leu Pro Thr His Gln 
                165                 170                 175     


Met Pro Leu Leu Ala Arg Gly Asn Phe Asn Pro Glu Phe Ile Ser Val 
            180                 185                 190         


Leu Ser His Lys Gln Asn Asp Thr Lys Lys Ser Lys Ile Lys Val Thr 
        195                 200                 205             


Tyr Gln Arg Glu Met Asp Arg Tyr Thr Asn Gln Trp Asn Arg Leu His 
    210                 215                 220                 


Trp Val Gly Asn Asn Tyr Lys Asn Gln Asn Thr Val Thr Phe Thr Ser 
225                 230                 235                 240 


Thr Tyr Glu Val Asp Trp Gln Asn His Thr Val Lys Leu Ile Gly Thr 
                245                 250                 255     


Asp Ser Lys Glu Thr Asn Pro Gly Val 
            260                 265 


<210>  4
<211>  283
<212>  PRT
<213>  Artificial

<220>
<223>  LukE (Delta)sp (signal peptide removed)

<400>  4

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly 
            100                 105                 110         


Tyr Asn Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn 
        115                 120                 125             


Gly Ser Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr 
    130                 135                 140                 


Val Ser Glu Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val 
145                 150                 155                 160 


Lys Ala Asn Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp 
                165                 170                 175     


Arg Tyr Leu Phe Val Gln Ser Pro Asn Gly Pro Thr Gly Ser Ala Arg 
            180                 185                 190         


Glu Tyr Phe Ala Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly 
        195                 200                 205             


Phe Asn Pro Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser 
    210                 215                 220                 


Asp Thr Ser Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr 
225                 230                 235                 240 


Tyr Ala Thr Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His 
                245                 250                 255     


Asn Ala Phe Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp 
            260                 265                 270         


Lys Thr His Glu Ile Lys Val Lys Gly His Asn 
        275                 280             


<210>  5
<211>  244
<212>  PRT
<213>  Artificial

<220>
<223>  lukE (Delta)sp(Delta)stem (signal peptide and stem removed)

<400>  5

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Ser Tyr Val Ser Glu Val Asp Lys Gln 
            100                 105                 110         


Asn Ser Lys Ser Val Lys Trp Gly Val Lys Ala Asn Glu Phe Val Thr 
        115                 120                 125             


Pro Asp Gly Lys Lys Ser Ala His Asp Arg Tyr Leu Phe Val Gln Ser 
    130                 135                 140                 


Pro Asn Gly Pro Thr Gly Ser Ala Arg Glu Tyr Phe Ala Pro Asp Asn 
145                 150                 155                 160 


Gln Leu Pro Pro Leu Val Gln Ser Gly Phe Asn Pro Ser Phe Ile Thr 
                165                 170                 175     


Thr Leu Ser His Glu Lys Gly Ser Ser Asp Thr Ser Glu Phe Glu Ile 
            180                 185                 190         


Ser Tyr Gly Arg Asn Leu Asp Ile Thr Tyr Ala Thr Leu Phe Pro Arg 
        195                 200                 205             


Thr Gly Ile Tyr Ala Glu Arg Lys His Asn Ala Phe Val Asn Arg Asn 
    210                 215                 220                 


Phe Val Val Arg Tyr Glu Val Asn Trp Lys Thr His Glu Ile Lys Val 
225                 230                 235                 240 


Lys Gly His Asn 
                


<210>  6
<211>  248
<212>  PRT
<213>  Artificial

<220>
<223>  lukE (Delta)sp(Delta)stem-PSGS (signal peptide removed, stem 
       replaced with PSGS-linker)

<400>  6

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Pro Ser Gly Ser Ser Tyr Val Ser Glu 
            100                 105                 110         


Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val Lys Ala Asn 
        115                 120                 125             


Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp Arg Tyr Leu 
    130                 135                 140                 


Phe Val Gln Ser Pro Asn Gly Pro Thr Gly Ser Ala Arg Glu Tyr Phe 
145                 150                 155                 160 


Ala Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly Phe Asn Pro 
                165                 170                 175     


Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser Asp Thr Ser 
            180                 185                 190         


Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr Tyr Ala Thr 
        195                 200                 205             


Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His Asn Ala Phe 
    210                 215                 220                 


Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp Lys Thr His 
225                 230                 235                 240 


Glu Ile Lys Val Lys Gly His Asn 
                245             


<210>  7
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Linker

<400>  7

Pro Ser Gly Ser 
1               


<210>  8
<211>  346
<212>  PRT
<213>  Artificial

<220>
<223>  ClfA N2N3 P116S/Y118A  with PglB consensus sequence (KDQNATK,) 
       substituted for residue I337

<400>  8

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345     


<210>  9
<211>  299
<212>  PRT
<213>  Artificial

<220>
<223>  Mature Hla H35L/H48C/G122C with PglB consensus sequence  
       (KDQNRTK) substituted for residue K131

<400>  9

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 


<210>  10
<211>  291
<212>  PRT
<213>  Artificial

<220>
<223>  SpA IgG binding portion

<400>  10

Ala Gln His Asp Glu Ala Lys Lys Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Lys Arg Asn Asn Phe 
    50                  55                  60                  


Asn Lys Asp Lys Lys Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
65                  70                  75                  80  


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala 
                85                  90                  95      


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
            100                 105                 110         


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Lys Lys Asn 
        115                 120                 125             


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
    130                 135                 140                 


Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn 
145                 150                 155                 160 


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
                165                 170                 175     


Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu 
            180                 185                 190         


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
        195                 200                 205             


Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
    210                 215                 220                 


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
225                 230                 235                 240 


Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
                245                 250                 255     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser 
            260                 265                 270         


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        275                 280                 285             


Ala Pro Lys 
    290     


<210>  11
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  tag

<400>  11

His Arg His Arg 
1               


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  12
gagacacggt ccagactcct                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  13
acgatccgaa gaccttcatc                                                   20


<210>  14
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  lukD Forward Primer

<400>  14
gaaagttaca gaactacgat tgatagaaaa aca                                    33


<210>  15
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  lukD Reverse Primer

<400>  15
attattcata attttgtgcg cctcaaca                                          28


<210>  16
<211>  14
<212>  DNA
<213>  Artificial

<220>
<223>  lukD Probe

<400>  16
ccccagccaa ttga                                                         14


<210>  17
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  lukE Forward Primer

<400>  17
gatgttggtc aaacattagg atataacatt g                                      31


<210>  18
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  lukE Reverse Primer

<400>  18
attgttttag aataattaaa tgagccattg cca                                    33


<210>  19
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  lukE Probe

<400>  19
ctgactggaa attacc                                                       16


<210>  20
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  gyrB Forward Primer

<400>  20
ggtgactgca ttgtcagatg taaac                                             25


<210>  21
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  gyrB Reverse Primer

<400>  21
ctgcttctaa accttctaat acttgtattt g                                      31


<210>  22
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  gyrB Probe

<400>  22
cccagcacca taatta                                                       16


<210>  23
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  23
tccagggcaa tactaatatt gaaaatattg gtgatggtg                              39


<210>  24
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  24
ttagtattgc cctggaagta caggttttcg                                        30


<210>  25
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  25
aaatagaaag ttatgtcagt gaagtagaca agcaaaac                               38


<210>  26
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  26
acataacttt ctattttgtt tttaggaagg taattgatta ag                          42


<210>  27
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  27
ctgtacttcc agggcgctca acatatcaca cctgtaagc                              39


<210>  28
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  28
attaagtcgc gttatactcc aggattagtt tctttagaat ccg                         43


<210>  29
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  29
aaaatgaaga aagttacaga actacgattg atagaaaaac                             40


<210>  30
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  30
taactttctt cattttgatt tttaggtgca tagtc                                  35


<210>  31
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  31
gaaccgtctg gctctgaaag ttacagaact acgattgata gaaaaac                     47


<210>  32
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  32
ttcagagcca gacggttcat tttgattttt aggtgcatag tc                          42


