SEQUENCE LISTING

<110>  BASF Agricultural Solutions Seed US LLC

<120>  Improved genome editing using paired nickases

<130>  191497WO01

<160>  60

<170>  According Wipo Std 25




<210> 1
<211> 9155
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02430 encoding a modified Cas9

<400> 1
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcctgc    420
aggcccgggt taattaagcg gccgcctgca gtgcagcgtg acccggtcgt gcccctctct    480
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac    540
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat    600
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt    660
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt    720
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc    780
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt    840
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta    900
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa    960
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata   1020
atgccagcct gttaaacgcc gtcgatcgac gagtctaacg gacaccaacc agcgaaccag   1080
cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct gcctctggac   1140
ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc cagaaattgc   1200
gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct ctcacggcac   1260
cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct cgcccgccgt   1320
aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc ggagcgcaca   1380
cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca aggtacgccg   1440
ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatgctta   1500
gggcccggta gttctacttc tgtccatgtt tgtgttagat ccgtgtttgt gttagatccg   1560
tgctactagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact   1620
tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg   1680
atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca   1740
atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt   1800
gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt ttcaaactac   1860
ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca tagttacgaa   1920
ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat gcgggtttta   1980
ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg tgtggttggg   2040
cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct ggtgtattta   2100
ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt taagatggat   2160
ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat gcatatacat   2220
gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct attataataa   2280
acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca tatgcagcag   2340
ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg gtactgtttc   2400
ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggaccatgg cacccaagaa   2460
gaagaggaag gtgggcatcc acggcgtccc agcagcaatg gacaagaagt actccatcgg   2520
cctcgacatc ggcaccaaca gcgtcggctg ggccgtgatc accgacgagt acaaggtgcc   2580
aagcaagaag ttcaaggtgc tgggcaacac cgaccgtcac tccatcaaga agaacctcat   2640
cggcgccctc ctcttcgact caggtgagac ggccgaggcg acaaggttga agaggacggc   2700
aagaagaagg tacacaagaa ggaagaacag gatctgctac ctccaggaga tcttcagcaa   2760
cgagatggcc aaggtggatg acagcttctt ccaccggcta gaggagagct tcttggtgga   2820
ggaggacaag aagcatgaga ggcatcccat cttcggcaac atcgtcgacg aggtggccta   2880
ccatgagaag taccccacca tctaccatct ccgcaagaag ctggtggact caacggacaa   2940
ggccgacctc cgcctcatct acctcgcctt ggcccacatg atcaagttca gaggccactt   3000
cctcatcgag ggcgacctca accctgacaa cagcgatgtg gacaagctct tcatccagct   3060
ggtgcagacc tacaaccagc tctttgagga gaaccccatc aacgcctccg gcgtcgacgc   3120
caaggccatc ctctcggcaa ggttgagcaa gtcaagaagg ctggagaacc tcatcgctca   3180
gctccccggc gagaagaaaa atggcctctt cggcaacctc atcgccttga gcctcggcct   3240
cacccccaac ttcaagtcaa actttgacct cgccgaggat gccaagctcc agctctccaa   3300
ggacacctac gacgacgacc tcgacaacct cctcgcccag atcggcgacc agtacgccga   3360
cctcttcctc gccgccaaga acctctcaga tgccatcctc ctgagcgaca tcctccgcgt   3420
caacaccgag atcaccaagg cgccgctctc cgcctccatg atcaagaggt atgatgagca   3480
ccatcaagat ctcaccctcc tcaaggcctt ggtgaggcag cagctacctg agaagtacaa   3540
ggagatcttc ttcgaccaga gcaagaatgg ctacgccggc tacatcgacg gcggcgcctc   3600
ccaagaagag ttctacaagt tcatcaagcc catcttggag aagatggatg gcaccgagga   3660
gctactggtg aagctcaaca gagaagatct cctcaggaag cagaggacct tcgacaacgg   3720
cagcatccct caccagatcc accttggtga gctccatgcc atcttgagaa ggcaagaaga   3780
cttctacccc ttcctcaagg acaacaggga gaagatcgag aagatattga cattcaggat   3840
cccctactac gtcggaccct tggcaagagg caactcaagg ttcgcctgga tgacaaggaa   3900
gagcgaggaa accatcaccc cctggaactt tgaggaggtg gtggacaagg gcgcctcagc   3960
tcagagcttc atcgagagga tgaccaactt cgacaagaac ctccccaacg agaaggtgct   4020
ccccaagcac agcctcctct acgagtactt caccgtctac aatgagctca ccaaggtcaa   4080
gtacgtcacc gagggcatga ggaagccggc cttcctctca ggagagcaga agaaggccat   4140
cgtcgacctc ctcttcaaga ccaacaggaa ggtcaccgtc aagcagctca aggaggacta   4200
cttcaagaag atcgagtgct tcgactctgt ggagatctcc ggcgtggagg acaggttcaa   4260
cgcctccctc ggcacctacc atgatctcct gaagatcatc aaggacaagg acttcctcga   4320
caacgaggag aacgaggaca tcttagaaga catcgtcctc accctcaccc tcttcgagga   4380
cagggagatg atcgaggaga ggctcaagac ctatgctcac ctctttgatg acaaggtgat   4440
gaagcagctc aagagaagaa ggtacaccgg ctggggccgc ctctcaagga agctcatcaa   4500
cggcatcagg gacaagcaga gcggcaagac catcctcgac ttcctcaaga gcgatggctt   4560
cgccaacagg aacttcatgc agctcatcca tgatgacagc ttgaccttca aggaggacat   4620
ccagaaggct caagtctccg gccaaggaga ttccctccat gagcacatcg ccaacctcgc   4680
cggctcaccg gccatcaaga agggcatcct ccagaccgtc aaggtggtgg atgagctggt   4740
gaaggtgatg ggccgtcaca agccggagaa catcgtcatc gagatggcaa gggagaacca   4800
gaccacccag aagggccaga agaactcaag ggagaggatg aagaggattg aagaaggcat   4860
caaggagctc ggcagccaga tcctcaagga gcaccctgtg gagaacaccc agctacagaa   4920
cgagaagctc tacctctact acctccagaa tggccgtgac atgtacgtcg accaagagct   4980
ggacatcaac aggttgagcg actacgacgt cgaccacatc gtcccccaga gcttcctcaa   5040
ggatgacagc atcgacaaca aggtgctgac aagatcagac aagaaccgtg gcaagagcga   5100
caatgtcccc tccgaggagg tggtgaagaa gatgaagaac tactggaggc agctcctcaa   5160
cgccaagctc atcacccaga ggaagttcga caacctcacc aaggctgaaa gaggaggcct   5220
ctcagagctg gacaaggccg gcttcatcaa gaggcagctg gtggagacaa ggcagatcac   5280
caagcatgtt gctcagatcc ttgattcaag gatgaacacc aagtacgacg agaacgacaa   5340
gctcatcagg gaggtcaagg tcatcaccct caagagcaag ctggtgtccg acttcaggaa   5400
ggacttccag ttctacaagg tgagggagat caacaactac caccatgctc atgatgccta   5460
cctcaacgcc gtggtgggca ccgccctcat caagaagtac cccaagctgg agtcagagtt   5520
cgtctacggc gactacaagg tgtatgatgt gaggaagatg atcgccaaga gcgagcagga   5580
gatcggcaag gccaccgcca agtacttctt ctactcaaac atcatgaact tcttcaagac   5640
cgagatcacc ctcgccaacg gcgagatcag gaagaggccg ctcattgaga caaatggtga   5700
gactggtgag atcgtctggg acaagggccg agacttcgcc accgtgagga aggtgctctc   5760
catgcctcaa gtcaacatcg tcaagaagac cgaggtgcag accggaggct tctcaaagga   5820
gagcatcctc cccaagagga actctgacaa gctgatcgca aggaagaagg actgggaccc   5880
caagaagtac ggcggctttg attcaccaac ggtggcctac tccgtgctgg tggtggccaa   5940
ggtggagaag ggcaagagca agaagctcaa gagcgtcaag gagctcctcg gcatcaccat   6000
catggagaga tcatccttcg agaagaaccc catcgacttc cttgaggcca agggctacaa   6060
ggaggtgaag aaggacctca tcatcaagct ccccaagtac agcctcttcg agctggagaa   6120
tggccgcaag aggatgctgg catcagctgg agagctccag aagggcaacg agctggccct   6180
cccaagcaag tacgtgaact tcctctacct cgcctcccac tacgagaagc tcaagggctc   6240
accagaagac aatgagcaga agcagctctt cgtggagcag cacaagcact acctcgacga   6300
gatcatcgag cagatctccg agttctccaa gagggtgatc ctcgccgacg ccaacctcga   6360
caaggtgctc tcagcctaca acaagcaccg tgacaagccc atcagggagc aagctgagaa   6420
catcatccac ctcttcaccc tcaccaacct cggcgccccg gcggccttca agtacttcga   6480
caccaccatc gaccgcaaga ggtacacctc aaccaaggag gtgcttgatg ccaccttgat   6540
ccaccagagc atcaccggcc tctacgagac aaggatcgac ctctcccagc tcggcggcga   6600
caagaggccg gcggccacca agaaggccgg ccaagccaag aagaagaagt gattgctagc   6660
acgcgttgga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc   6720
tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc   6780
tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat   6840
caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cggcgcgcca   6900
tcccaatggc gcgccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg   6960
ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg   7020
tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc   7080
gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt   7140
gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct   7200
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga   7260
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc   7320
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg   7380
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg   7440
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt   7500
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt   7560
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg   7620
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact   7680
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt   7740
cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct   7800
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac   7860
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc   7920
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg   7980
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta   8040
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca   8100
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc   8160
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc   8220
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc   8280
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat   8340
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt   8400
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc   8460
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag   8520
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt   8580
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac   8640
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg   8700
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat   8760
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc   8820
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc   8880
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa   8940
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg   9000
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   9060
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   9120
ctataaaaat aggcgtatca cgaggccctt tcgtc                              9155

<210> 2
<211> 1401
<212> PRT
<213> Artificial sequence

<220>
<223> Modified Cas9 from Plasmid BAY02430

<400> 2
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1               5                   10                  15
Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser
            20                  25                  30
Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys
        35                  40                  45
Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu
    50                  55                  60
Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg
65                  70                  75                  80
Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile
                85                  90                  95
Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp
            100                 105                 110
Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys
        115                 120                 125
Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala
    130                 135                 140
Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val
145                 150                 155                 160
Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala
                165                 170                 175
His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn
            180                 185                 190
Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr
        195                 200                 205
Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp
    210                 215                 220
Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu
225                 230                 235                 240
Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly
                245                 250                 255
Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn
            260                 265                 270
Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr
        275                 280                 285
Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala
    290                 295                 300
Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser
305                 310                 315                 320
Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala
                325                 330                 335
Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu
            340                 345                 350
Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe
        355                 360                 365
Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala
    370                 375                 380
Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met
385                 390                 395                 400
Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu
                405                 410                 415
Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His
            420                 425                 430
Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro
        435                 440                 445
Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg
    450                 455                 460
Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala
465                 470                 475                 480
Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu
                485                 490                 495
Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met
            500                 505                 510
Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His
        515                 520                 525
Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val
    530                 535                 540
Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu
545                 550                 555                 560
Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val
                565                 570                 575
Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe
            580                 585                 590
Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
        595                 600                 605
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu
    610                 615                 620
Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu
625                 630                 635                 640
Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr
                645                 650                 655
Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg
            660                 665                 670
Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg
        675                 680                 685
Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly
    690                 695                 700
Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr
705                 710                 715                 720
Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser
                725                 730                 735
Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys
            740                 745                 750
Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met
        755                 760                 765
Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn
    770                 775                 780
Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg
785                 790                 795                 800
Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His
                805                 810                 815
Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr
            820                 825                 830
Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
        835                 840                 845
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu
    850                 855                 860
Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn
865                 870                 875                 880
Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met
                885                 890                 895
Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg
            900                 905                 910
Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu
        915                 920                 925
Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
    930                 935                 940
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr
945                 950                 955                 960
Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys
                965                 970                 975
Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val
            980                 985                 990
Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala
        995                 1000                1005
Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu
    1010                1015                1020
Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala
1025                1030                1035                1040
Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr
                1045                1050                1055
Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly
            1060                1065                1070
Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
        1075                1080                1085
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu
    1090                1095                1100
Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly
1105                1110                1115                1120
Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu
                1125                1130                1135
Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp
            1140                1145                1150
Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys
        1155                1160                1165
Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
    1170                1175                1180
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu
1185                1190                1195                1200
Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro
                1205                1210                1215
Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala
            1220                1225                1230
Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys
        1235                1240                1245
Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly
    1250                1255                1260
Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys
1265                1270                1275                1280
His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg
                1285                1290                1295
Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn
            1300                1305                1310
Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
        1315                1320                1325
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe
    1330                1335                1340
Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu
1345                1350                1355                1360
Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg
                1365                1370                1375
Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala Thr Lys
            1380                1385                1390
Lys Ala Gly Gln Ala Lys Lys Lys Lys
        1395                1400

<210> 3
<211> 9155
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02734 encoding Cas9 D10A nickase

<400> 3
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcctgc    420
aggcccgggt taattaagcg gccgcctgca gtgcagcgtg acccggtcgt gcccctctct    480
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac    540
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat    600
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt    660
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt    720
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc    780
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt    840
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta    900
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa    960
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata   1020
atgccagcct gttaaacgcc gtcgatcgac gagtctaacg gacaccaacc agcgaaccag   1080
cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct gcctctggac   1140
ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc cagaaattgc   1200
gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct ctcacggcac   1260
cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct cgcccgccgt   1320
aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc ggagcgcaca   1380
cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca aggtacgccg   1440
ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatgctta   1500
gggcccggta gttctacttc tgtccatgtt tgtgttagat ccgtgtttgt gttagatccg   1560
tgctactagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact   1620
tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg   1680
atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca   1740
atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt   1800
gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt ttcaaactac   1860
ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca tagttacgaa   1920
ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat gcgggtttta   1980
ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg tgtggttggg   2040
cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct ggtgtattta   2100
ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt taagatggat   2160
ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat gcatatacat   2220
gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct attataataa   2280
acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca tatgcagcag   2340
ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg gtactgtttc   2400
ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggaccatgg cacccaagaa   2460
gaagaggaag gtgggcatcc acggcgtccc agcagcaatg gacaagaagt actccatcgg   2520
cctcgccatc ggcaccaaca gcgtcggctg ggccgtgatc accgacgagt acaaggtgcc   2580
aagcaagaag ttcaaggtgc tgggcaacac cgaccgtcac tccatcaaga agaacctcat   2640
cggcgccctc ctcttcgact caggtgagac ggccgaggcg acaaggttga agaggacggc   2700
aagaagaagg tacacaagaa ggaagaacag gatctgctac ctccaggaga tcttcagcaa   2760
cgagatggcc aaggtggatg acagcttctt ccaccggcta gaggagagct tcttggtgga   2820
ggaggacaag aagcatgaga ggcatcccat cttcggcaac atcgtcgacg aggtggccta   2880
ccatgagaag taccccacca tctaccatct ccgcaagaag ctggtggact caacggacaa   2940
ggccgacctc cgcctcatct acctcgcctt ggcccacatg atcaagttca gaggccactt   3000
cctcatcgag ggcgacctca accctgacaa cagcgatgtg gacaagctct tcatccagct   3060
ggtgcagacc tacaaccagc tctttgagga gaaccccatc aacgcctccg gcgtcgacgc   3120
caaggccatc ctctcggcaa ggttgagcaa gtcaagaagg ctggagaacc tcatcgctca   3180
gctccccggc gagaagaaaa atggcctctt cggcaacctc atcgccttga gcctcggcct   3240
cacccccaac ttcaagtcaa actttgacct cgccgaggat gccaagctcc agctctccaa   3300
ggacacctac gacgacgacc tcgacaacct cctcgcccag atcggcgacc agtacgccga   3360
cctcttcctc gccgccaaga acctctcaga tgccatcctc ctgagcgaca tcctccgcgt   3420
caacaccgag atcaccaagg cgccgctctc cgcctccatg atcaagaggt atgatgagca   3480
ccatcaagat ctcaccctcc tcaaggcctt ggtgaggcag cagctacctg agaagtacaa   3540
ggagatcttc ttcgaccaga gcaagaatgg ctacgccggc tacatcgacg gcggcgcctc   3600
ccaagaagag ttctacaagt tcatcaagcc catcttggag aagatggatg gcaccgagga   3660
gctactggtg aagctcaaca gagaagatct cctcaggaag cagaggacct tcgacaacgg   3720
cagcatccct caccagatcc accttggtga gctccatgcc atcttgagaa ggcaagaaga   3780
cttctacccc ttcctcaagg acaacaggga gaagatcgag aagatattga cattcaggat   3840
cccctactac gtcggaccct tggcaagagg caactcaagg ttcgcctgga tgacaaggaa   3900
gagcgaggaa accatcaccc cctggaactt tgaggaggtg gtggacaagg gcgcctcagc   3960
tcagagcttc atcgagagga tgaccaactt cgacaagaac ctccccaacg agaaggtgct   4020
ccccaagcac agcctcctct acgagtactt caccgtctac aatgagctca ccaaggtcaa   4080
gtacgtcacc gagggcatga ggaagccggc cttcctctca ggagagcaga agaaggccat   4140
cgtcgacctc ctcttcaaga ccaacaggaa ggtcaccgtc aagcagctca aggaggacta   4200
cttcaagaag atcgagtgct tcgactctgt ggagatctcc ggcgtggagg acaggttcaa   4260
cgcctccctc ggcacctacc atgatctcct gaagatcatc aaggacaagg acttcctcga   4320
caacgaggag aacgaggaca tcttagaaga catcgtcctc accctcaccc tcttcgagga   4380
cagggagatg atcgaggaga ggctcaagac ctatgctcac ctctttgatg acaaggtgat   4440
gaagcagctc aagagaagaa ggtacaccgg ctggggccgc ctctcaagga agctcatcaa   4500
cggcatcagg gacaagcaga gcggcaagac catcctcgac ttcctcaaga gcgatggctt   4560
cgccaacagg aacttcatgc agctcatcca tgatgacagc ttgaccttca aggaggacat   4620
ccagaaggct caagtctccg gccaaggaga ttccctccat gagcacatcg ccaacctcgc   4680
cggctcaccg gccatcaaga agggcatcct ccagaccgtc aaggtggtgg atgagctggt   4740
gaaggtgatg ggccgtcaca agccggagaa catcgtcatc gagatggcaa gggagaacca   4800
gaccacccag aagggccaga agaactcaag ggagaggatg aagaggattg aagaaggcat   4860
caaggagctc ggcagccaga tcctcaagga gcaccctgtg gagaacaccc agctacagaa   4920
cgagaagctc tacctctact acctccagaa tggccgtgac atgtacgtcg accaagagct   4980
ggacatcaac aggttgagcg actacgacgt cgaccacatc gtcccccaga gcttcctcaa   5040
ggatgacagc atcgacaaca aggtgctgac aagatcagac aagaaccgtg gcaagagcga   5100
caatgtcccc tccgaggagg tggtgaagaa gatgaagaac tactggaggc agctcctcaa   5160
cgccaagctc atcacccaga ggaagttcga caacctcacc aaggctgaaa gaggaggcct   5220
ctcagagctg gacaaggccg gcttcatcaa gaggcagctg gtggagacaa ggcagatcac   5280
caagcatgtt gctcagatcc ttgattcaag gatgaacacc aagtacgacg agaacgacaa   5340
gctcatcagg gaggtcaagg tcatcaccct caagagcaag ctggtgtccg acttcaggaa   5400
ggacttccag ttctacaagg tgagggagat caacaactac caccatgctc atgatgccta   5460
cctcaacgcc gtggtgggca ccgccctcat caagaagtac cccaagctgg agtcagagtt   5520
cgtctacggc gactacaagg tgtatgatgt gaggaagatg atcgccaaga gcgagcagga   5580
gatcggcaag gccaccgcca agtacttctt ctactcaaac atcatgaact tcttcaagac   5640
cgagatcacc ctcgccaacg gcgagatcag gaagaggccg ctcattgaga caaatggtga   5700
gactggtgag atcgtctggg acaagggccg agacttcgcc accgtgagga aggtgctctc   5760
catgcctcaa gtcaacatcg tcaagaagac cgaggtgcag accggaggct tctcaaagga   5820
gagcatcctc cccaagagga actctgacaa gctgatcgca aggaagaagg actgggaccc   5880
caagaagtac ggcggctttg attcaccaac ggtggcctac tccgtgctgg tggtggccaa   5940
ggtggagaag ggcaagagca agaagctcaa gagcgtcaag gagctcctcg gcatcaccat   6000
catggagaga tcatccttcg agaagaaccc catcgacttc cttgaggcca agggctacaa   6060
ggaggtgaag aaggacctca tcatcaagct ccccaagtac agcctcttcg agctggagaa   6120
tggccgcaag aggatgctgg catcagctgg agagctccag aagggcaacg agctggccct   6180
cccaagcaag tacgtgaact tcctctacct cgcctcccac tacgagaagc tcaagggctc   6240
accagaagac aatgagcaga agcagctctt cgtggagcag cacaagcact acctcgacga   6300
gatcatcgag cagatctccg agttctccaa gagggtgatc ctcgccgacg ccaacctcga   6360
caaggtgctc tcagcctaca acaagcaccg tgacaagccc atcagggagc aagctgagaa   6420
catcatccac ctcttcaccc tcaccaacct cggcgccccg gcggccttca agtacttcga   6480
caccaccatc gaccgcaaga ggtacacctc aaccaaggag gtgcttgatg ccaccttgat   6540
ccaccagagc atcaccggcc tctacgagac aaggatcgac ctctcccagc tcggcggcga   6600
caagaggccg gcggccacca agaaggccgg ccaagccaag aagaagaagt gattgctagc   6660
acgcgttgga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc   6720
tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc   6780
tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat   6840
caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cggcgcgcca   6900
tcccaatggc gcgccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg   6960
ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg   7020
tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc   7080
gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt   7140
gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct   7200
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga   7260
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc   7320
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg   7380
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg   7440
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt   7500
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt   7560
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg   7620
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact   7680
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt   7740
cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct   7800
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac   7860
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc   7920
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg   7980
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta   8040
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca   8100
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc   8160
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc   8220
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc   8280
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat   8340
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt   8400
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc   8460
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag   8520
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt   8580
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac   8640
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg   8700
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat   8760
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc   8820
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc   8880
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa   8940
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg   9000
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   9060
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   9120
ctataaaaat aggcgtatca cgaggccctt tcgtc                              9155

<210> 4
<211> 1401
<212> PRT
<213> Artificial sequence

<220>
<223> Cas9 D10A nickase from Plasmid BAY02734

<400> 4
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1               5                   10                  15
Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser
            20                  25                  30
Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys
        35                  40                  45
Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu
    50                  55                  60
Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg
65                  70                  75                  80
Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile
                85                  90                  95
Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp
            100                 105                 110
Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys
        115                 120                 125
Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala
    130                 135                 140
Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val
145                 150                 155                 160
Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala
                165                 170                 175
His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn
            180                 185                 190
Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr
        195                 200                 205
Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp
    210                 215                 220
Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu
225                 230                 235                 240
Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly
                245                 250                 255
Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn
            260                 265                 270
Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr
        275                 280                 285
Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala
    290                 295                 300
Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser
305                 310                 315                 320
Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala
                325                 330                 335
Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu
            340                 345                 350
Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe
        355                 360                 365
Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala
    370                 375                 380
Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met
385                 390                 395                 400
Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu
                405                 410                 415
Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His
            420                 425                 430
Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro
        435                 440                 445
Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg
    450                 455                 460
Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala
465                 470                 475                 480
Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu
                485                 490                 495
Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met
            500                 505                 510
Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His
        515                 520                 525
Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val
    530                 535                 540
Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu
545                 550                 555                 560
Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val
                565                 570                 575
Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe
            580                 585                 590
Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
        595                 600                 605
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu
    610                 615                 620
Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu
625                 630                 635                 640
Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr
                645                 650                 655
Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg
            660                 665                 670
Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg
        675                 680                 685
Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly
    690                 695                 700
Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr
705                 710                 715                 720
Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser
                725                 730                 735
Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys
            740                 745                 750
Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met
        755                 760                 765
Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn
    770                 775                 780
Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg
785                 790                 795                 800
Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His
                805                 810                 815
Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr
            820                 825                 830
Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
        835                 840                 845
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu
    850                 855                 860
Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn
865                 870                 875                 880
Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met
                885                 890                 895
Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg
            900                 905                 910
Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu
        915                 920                 925
Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
    930                 935                 940
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr
945                 950                 955                 960
Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys
                965                 970                 975
Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val
            980                 985                 990
Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala
        995                 1000                1005
Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu
    1010                1015                1020
Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala
1025                1030                1035                1040
Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr
                1045                1050                1055
Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly
            1060                1065                1070
Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
        1075                1080                1085
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu
    1090                1095                1100
Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly
1105                1110                1115                1120
Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu
                1125                1130                1135
Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp
            1140                1145                1150
Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys
        1155                1160                1165
Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
    1170                1175                1180
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu
1185                1190                1195                1200
Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro
                1205                1210                1215
Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala
            1220                1225                1230
Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys
        1235                1240                1245
Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly
    1250                1255                1260
Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys
1265                1270                1275                1280
His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg
                1285                1290                1295
Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn
            1300                1305                1310
Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
        1315                1320                1325
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe
    1330                1335                1340
Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu
1345                1350                1355                1360
Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg
                1365                1370                1375
Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala Thr Lys
            1380                1385                1390
Lys Ala Gly Gln Ala Lys Lys Lys Lys
        1395                1400

<210> 5
<211> 3120
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02528 comprising guide RNA

<400> 5
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ttaattaaaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc    480
cttattttaa cttgctattt ctagctctaa aactgtatgt tctccacacc tagcaagtct    540
gatgcagcaa gcgagtttat aaaccggtca gaactctgct ggacaggcga ggtgggacta    600
aactaagagg aactgctgcg ttgctctccc caatcgtgaa cacgtcgacc acccgacaaa    660
agcccgatca tgcgggccta aaagcccagc gctagaccgt tgctgaactc acgtgggctg    720
catctcacta tgcgacccag atcagtgaga gctagagggc agttatcttc agctctctcg    780
tagttcctct gatcaatatt cctagttcga ttccctccta gcagtgagta acaatgtgct    840
ccttgataaa tataaatgtg ttgagcacca gaactgtcag aataacgggc ttggtccctg    900
cagggttcca tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg    960
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta   1020
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg   1080
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga   1140
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg   1200
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag   1260
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc   1320
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca   1380
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt   1440
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc   1500
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc   1560
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc   1620
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact   1680
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg   1740
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta   1800
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca   1860
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa   1920
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg   1980
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc   2040
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg   2100
acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat   2160
caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt   2220
tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac   2280
aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga   2340
cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag   2400
gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg   2460
attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa   2520
tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag   2580
gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg   2640
catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc   2700
agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca   2760
gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc   2820
cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc   2880
gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt   2940
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga   3000
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag   3060
aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc   3120

<210> 6
<211> 3119
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02529 comprising guide RNA

<400> 6
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ttaattaaaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc    480
cttattttaa cttgctattt ctagctctaa aaccacctag cccatcctcc ttccaagtct    540
gatgcagcaa gcgagtttat aaaccggtca gaactctgct ggacaggcga ggtgggacta    600
aactaagagg aactgctgcg ttgctctccc caatcgtgaa cacgtcgacc acccgacaaa    660
agcccgatca tgcgggccta aaagcccagc gctagaccgt tgctgaactc acgtgggctg    720
catctcacta tgcgacccag atcagtgaga gctagagggc agttatcttc agctctctcg    780
tagttcctct gatcaatatt cctagttcga ttccctccta gcagtgagta acaatgtgct    840
ccttgataaa tataaatgtg ttgagcacca gaactgtcag aataacgggc ttggtccctg    900
cagggttcat cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt    960
gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa   1020
agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc   1080
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag   1140
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   1200
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   1260
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   1320
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   1380
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   1440
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   1500
gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   1560
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   1620
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   1680
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   1740
tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   1800
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   1860
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   1920
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   1980
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   2040
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   2100
cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc   2160
aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt   2220
ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca   2280
acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac   2340
gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg   2400
ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga   2460
ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat   2520
cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg   2580
atattcttct aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc   2640
atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca   2700
gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag   2760
aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc   2820
gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg   2880
cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta   2940
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa   3000
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga   3060
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc    3119

<210> 7
<211> 3117
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02530 comprising guide RNA

<400> 7
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggataaccc    420
tgcagggacc aagcccgtta ttctgacagt tctggtgctc aacacattta tatttatcaa    480
ggagcacatt gttactcact gctaggaggg aatcgaacta ggaatattga tcagaggaac    540
tacgagagag ctgaagataa ctgccctcta gctctcactg atctgggtcg catagtgaga    600
tgcagcccac gtgagttcag caacggtcta gcgctgggct tttaggcccg catgatcggg    660
cttttgtcgg gtggtcgacg tgttcacgat tggggagagc aacgcagcag ttcctcttag    720
tttagtccca cctcgcctgt ccagcagagt tctgaccggt ttataaactc gcttgctgca    780
tcagacttga taggccctag aataggcacg ttttagagct agaaatagca agttaaaata    840
aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttttttaatt    900
aagttcatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt    960
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag   1020
cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt   1080
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag   1140
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg   1200
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat   1260
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta   1320
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa   1380
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc   1440
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt   1500
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca   1560
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg   1620
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat   1680
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta   1740
cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct   1800
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac   1860
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa   1920
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa   1980
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt   2040
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca   2100
gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa   2160
taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc   2220
ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac   2280
ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga   2340
ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc   2400
agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt   2460
gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg   2520
aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat   2580
attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt aaccatgcat   2640
catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt   2700
ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa   2760
acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga   2820
cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg   2880
gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt   2940
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa   3000
ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa   3060
ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc      3117

<210> 8
<211> 3117
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02531 comprising guide RNA

<400> 8
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
cctgcaggga ccaagcccgt tattctgaca gttctggtgc tcaacacatt tatatttatc    480
aaggagcaca ttgttactca ctgctaggag ggaatcgaac taggaatatt gatcagagga    540
actacgagag agctgaagat aactgccctc tagctctcac tgatctgggt cgcatagtga    600
gatgcagccc acgtgagttc agcaacggtc tagcgctggg cttttaggcc cgcatgatcg    660
ggcttttgtc gggtggtcga cgtgttcacg attggggaga gcaacgcagc agttcctctt    720
agtttagtcc cacctcgcct gtccagcaga gttctgaccg gtttataaac tcgcttgctg    780
catcagactt gctcctcata ggccctagaa tgttttagag ctagaaatag caagttaaaa    840
taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttttttaa    900
ttaagtatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt    960
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag   1020
cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt   1080
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag   1140
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg   1200
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat   1260
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta   1320
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa   1380
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc   1440
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt   1500
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca   1560
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg   1620
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat   1680
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta   1740
cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct   1800
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac   1860
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa   1920
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa   1980
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt   2040
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca   2100
gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa   2160
taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc   2220
ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac   2280
ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga   2340
ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc   2400
agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt   2460
gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg   2520
aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat   2580
attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt aaccatgcat   2640
catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt   2700
ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa   2760
acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga   2820
cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg   2880
gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt   2940
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa   3000
ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa   3060
ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc      3117

<210> 9
<211> 3119
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02532 comprising guide RNA

<400> 9
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgaacc    420
ctgcagggac caagcccgtt attctgacag ttctggtgct caacacattt atatttatca    480
aggagcacat tgttactcac tgctaggagg gaatcgaact aggaatattg atcagaggaa    540
ctacgagaga gctgaagata actgccctct agctctcact gatctgggtc gcatagtgag    600
atgcagccca cgtgagttca gcaacggtct agcgctgggc ttttaggccc gcatgatcgg    660
gcttttgtcg ggtggtcgac gtgttcacga ttggggagag caacgcagca gttcctctta    720
gtttagtccc acctcgcctg tccagcagag ttctgaccgg tttataaact cgcttgctgc    780
atcagacttg ctattgccag tgcctattct gttttagagc tagaaatagc aagttaaaat    840
aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttttttaat    900
taagttccat cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt    960
gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa   1020
agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc   1080
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag   1140
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   1200
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   1260
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   1320
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   1380
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   1440
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   1500
gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   1560
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   1620
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   1680
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   1740
tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   1800
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   1860
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   1920
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   1980
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   2040
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   2100
cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc   2160
aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt   2220
ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca   2280
acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac   2340
gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg   2400
ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga   2460
ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat   2520
cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg   2580
atattcttct aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc   2640
atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca   2700
gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag   2760
aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc   2820
gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg   2880
cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta   2940
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa   3000
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga   3060
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc    3119

<210> 10
<211> 3119
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02524 comprising guide RNA

<400> 10
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgaact    420
taattaaaaa aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc    480
ttattttaac ttgctatttc tagctctaaa acgacttgcc cagcacgagg aacaagtctg    540
atgcagcaag cgagtttata aaccggtcag aactctgctg gacaggcgag gtgggactaa    600
actaagagga actgctgcgt tgctctcccc aatcgtgaac acgtcgacca cccgacaaaa    660
gcccgatcat gcgggcctaa aagcccagcg ctagaccgtt gctgaactca cgtgggctgc    720
atctcactat gcgacccaga tcagtgagag ctagagggca gttatcttca gctctctcgt    780
agttcctctg atcaatattc ctagttcgat tccctcctag cagtgagtaa caatgtgctc    840
cttgataaat ataaatgtgt tgagcaccag aactgtcaga ataacgggct tggtccctgc    900
agggttccat cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt    960
gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa   1020
agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc   1080
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag   1140
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   1200
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   1260
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   1320
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   1380
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   1440
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   1500
gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   1560
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   1620
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   1680
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   1740
tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   1800
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   1860
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   1920
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   1980
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   2040
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   2100
cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc   2160
aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt   2220
ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca   2280
acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac   2340
gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg   2400
ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga   2460
ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat   2520
cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg   2580
atattcttct aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc   2640
atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca   2700
gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag   2760
aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc   2820
gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg   2880
cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta   2940
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa   3000
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga   3060
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc    3119

<210> 11
<211> 3120
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02533 comprising guide RNA

<400> 11
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
cctgcaggga ccaagcccgt tattctgaca gttctggtgc tcaacacatt tatatttatc    480
aaggagcaca ttgttactca ctgctaggag ggaatcgaac taggaatatt gatcagagga    540
actacgagag agctgaagat aactgccctc tagctctcac tgatctgggt cgcatagtga    600
gatgcagccc acgtgagttc agcaacggtc tagcgctggg cttttaggcc cgcatgatcg    660
ggcttttgtc gggtggtcga cgtgttcacg attggggaga gcaacgcagc agttcctctt    720
agtttagtcc cacctcgcct gtccagcaga gttctgaccg gtttataaac tcgcttgctg    780
catcagactt ggaacaacca gcatctggga agttttagag ctagaaatag caagttaaaa    840
taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttttttaa    900
ttaagttcca tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg    960
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta   1020
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg   1080
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga   1140
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg   1200
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag   1260
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc   1320
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca   1380
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt   1440
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc   1500
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc   1560
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc   1620
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact   1680
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg   1740
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta   1800
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca   1860
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa   1920
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg   1980
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc   2040
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg   2100
acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat   2160
caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt   2220
tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac   2280
aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga   2340
cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag   2400
gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg   2460
attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa   2520
tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag   2580
gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg   2640
catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc   2700
agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca   2760
gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc   2820
cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc   2880
gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt   2940
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga   3000
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag   3060
aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc   3120

<210> 12
<211> 3120
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02535 comprising guide RNA

<400> 12
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
cctgcaggga ccaagcccgt tattctgaca gttctggtgc tcaacacatt tatatttatc    480
aaggagcaca ttgttactca ctgctaggag ggaatcgaac taggaatatt gatcagagga    540
actacgagag agctgaagat aactgccctc tagctctcac tgatctgggt cgcatagtga    600
gatgcagccc acgtgagttc agcaacggtc tagcgctggg cttttaggcc cgcatgatcg    660
ggcttttgtc gggtggtcga cgtgttcacg attggggaga gcaacgcagc agttcctctt    720
agtttagtcc cacctcgcct gtccagcaga gttctgaccg gtttataaac tcgcttgctg    780
catcagactt gatctgggaa tggtggtgca ggttttagag ctagaaatag caagttaaaa    840
taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttttttaa    900
ttaagttcca tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg    960
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta   1020
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg   1080
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga   1140
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg   1200
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag   1260
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc   1320
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca   1380
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt   1440
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc   1500
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc   1560
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc   1620
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact   1680
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg   1740
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta   1800
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca   1860
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa   1920
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg   1980
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc   2040
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg   2100
acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat   2160
caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt   2220
tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac   2280
aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga   2340
cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag   2400
gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg   2460
attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa   2520
tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag   2580
gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg   2640
catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc   2700
agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca   2760
gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc   2820
cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc   2880
gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt   2940
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga   3000
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag   3060
aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc   3120

<210> 13
<211> 3455
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02539 comprising donor DNA

<400> 13
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ggcgcgccat gtattctcag ggatgtaagc aacgggtctg tcaggtgggt ccaaagattt    480
tgtaatagga agaggtccac caatgttggc aggaacatag ctgagccacc tcaatatatt    540
agatacacct tcaaggtcat ctgaaactgt cagatggaca acaccgtttg tcgccataat    600
tttggggcca cccaactgca tgtgggagct gtaaacttcc cggccaagaa gcttgttcaa    660
ggcagagaac ccagttagga taatgggctg gtcagtacgc tgtatgcacc gtatgccaag    720
tcgagcaaga tatgctccta ttccaacagt ccgtccagtc acaaatgtaa gcgtaaatgt    780
ctcctcatag gccctagaat aggcactggc aatagcagca cttccatgga ggttctcgac    840
gcccaaccca tcctccttcc ccacaacaga atcgataacc cacctaattt caccattatc    900
aagctgcatc ttgtgcgcta taacagaagt gctaatacga gcatggtctt cttcagtcag    960
ataaatgtac tgaaacccac gttcagggct gccatcatca gaccatccaa cacggaagca   1020
agattttact tcatctgcta tgccgatccg agcaccagag tttgctgcca agtagatgag   1080
aggaagcttc ctctcacaag ctaggttggt aacagtttca aaaaatgcat cttcccttgg   1140
accaaacgat ccagctctaa aagtaatatc atttgcgatg acaacaatct gcctgccatt   1200
gggatattca ggagtggaca tgtccaagat cttaattaag ttccatccca atggcgcgcc   1260
gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat   1320
tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag   1380
ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg   1440
ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc   1500
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc   1560
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa   1620
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt   1680
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg   1740
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg   1800
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag   1860
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc   1920
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa   1980
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg   2040
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc   2100
taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac   2160
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg   2220
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt   2280
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt   2340
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa   2400
atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat   2460
caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg   2520
tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta   2580
tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa   2640
aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa   2700
aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa   2760
atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac   2820
gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac   2880
tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc   2940
tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg   3000
cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt   3060
aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt   3120
cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata   3180
cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg   3240
ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg   3300
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   3360
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   3420
ctataaaaat aggcgtatca cgaggccctt tcgtc                              3455

<210> 14
<211> 3455
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02540 comprising donor DNA

<400> 14
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ttaattaaga tcttggacat gtccactcct gaatatccca atggcaggca gattgttgtc    480
atcgcaaatg atattacttt tagagctgga tcgtttggtc caagggaaga tgcatttttt    540
gaaactgtta ccaacctagc ttgtgagagg aagcttcctc tcatctactt ggcagcaaac    600
tctggtgctc ggatcggcat agcagatgaa gtaaaatctt gcttccgtgt tggatggtct    660
gatgatggca gccctgaacg tgggtttcag tacatttatc tgactgaaga agaccatgct    720
cgtattagca cttctgttat agcgcacaag atgcagcttg ataatggtga aattaggtgg    780
gttatcgatt ctgttgtggg gaaggaggat gggctaggtg tggagaacct ccatggaagt    840
gctgctattg cgagtgcgta tagccgcgcc tatgaggaga catttacgct tacatttgtg    900
actggacgga ctgttggaat aggagcatat cttgctcgac ttggcatacg gtgcatacag    960
cgtactgacc agcccattat cctaactggg ttctctgcct tgaacaagct tcttggccgg   1020
gaagtttaca gctcccacat gcagttgggt ggccccaaaa ttatggcgac aaacggtgtt   1080
gtccatctga cagtttcaga tgaccttgaa ggtgtatcta atatattgag gtggctcagc   1140
tatgttcctg ccaacattgg tggacctctt cctattacaa aatctttgga cccacctgac   1200
agacccgttg cttacatccc tgagaataca tggcgcgccg ttccatccca atggcgcgcc   1260
gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat   1320
tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag   1380
ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg   1440
ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc   1500
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc   1560
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa   1620
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt   1680
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg   1740
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg   1800
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag   1860
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc   1920
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa   1980
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg   2040
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc   2100
taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac   2160
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg   2220
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt   2280
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt   2340
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa   2400
atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat   2460
caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg   2520
tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta   2580
tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa   2640
aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa   2700
aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa   2760
atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac   2820
gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac   2880
tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc   2940
tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg   3000
cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt   3060
aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt   3120
cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata   3180
cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg   3240
ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg   3300
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   3360
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   3420
ctataaaaat aggcgtatca cgaggccctt tcgtc                              3455

<210> 15
<211> 3455
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02541 comprising donor DNA

<400> 15
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ggcgcgccat gtattctcag ggatgtaagc aacgggtctg tcaggtgggt ccaaagattt    480
tgtaatagga agaggtccac caatgttggc aggaacatag ctgagccacc tcaatatatt    540
agatacacct tcaaggtcat ctgaaactgt cagatggaca acaccgtttg tcgccataat    600
tttggggcca cccaactgca tgtgggagct gtaaacttcc cggccaagaa gcttgttcaa    660
ggcagagaac ccagttagga taatgggctg gtcagtacgc tgtatgcacc gtatgccaag    720
tcgagcaaga tatgctccta ttccaacagt ccgtccagtc acaaatgtaa gcgtaaatgt    780
ctcctcatag gcgcggctat acgcactcgc aatagcaaca cttccatgga ggttctccac    840
acctagccca tcctccttcc ccacaacaga atcgataacc cacctaattt caccattatc    900
aagctgcatc ttgtgcgcta taacagaagt gctaatacga gcatggtctt cttcagtcag    960
ataaatgtac tgaaacccac gttcagggct gccatcatca gaccatccaa cacggaagca   1020
agattttact tcatctgcta tgccgatccg agcaccagag tttgctgcca agtagatgag   1080
aggaagcttc ctctcacaag ctaggttggt aacagtttca aaaaatgcat cttcccttgg   1140
accaaacgat ccagctctaa aagtaatatc atttgcgatg acaacaatct gcctgccatt   1200
gggatattca ggagtggaca tgtccaagat cttaattaag ttccatccca atggcgcgcc   1260
gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat   1320
tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag   1380
ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg   1440
ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc   1500
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc   1560
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa   1620
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt   1680
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg   1740
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg   1800
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag   1860
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc   1920
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa   1980
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg   2040
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc   2100
taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac   2160
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg   2220
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt   2280
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt   2340
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa   2400
atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat   2460
caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg   2520
tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta   2580
tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa   2640
aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa   2700
aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa   2760
atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac   2820
gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac   2880
tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc   2940
tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg   3000
cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt   3060
aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt   3120
cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata   3180
cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg   3240
ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg   3300
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   3360
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   3420
ctataaaaat aggcgtatca cgaggccctt tcgtc                              3455

<210> 16
<211> 3439
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02536 comprising donor DNA

<400> 16
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ggcgcgccgg agctttgcct tcagatttat caattctgga tcaagcctac ccatgcactc    480
ttggagttcc tctgacctga acttgatctc gatcaaccct tgaggttcga gaacattgcc    540
ctttgcagtc ctctcagcat agaactcaat gcgatctgga tttatcttgc tatcaatcac    600
gacccaagcc cctccacgta gctctgcagc cttggggata tatacaaagg caggctgatt    660
gtatgtccta aggttctcaa caattgttga cccagcctga aggattcctt caaaaagatc    720
tctttgtcca ccagagaagc ctctccagtt agcaaggatg aacagaggta atccttcacg    780
gttgaagtcc agcattgcct gcgctgtctt cgtgacgctg tccgggaacc acacctgccc    840
agcacgagga acagatcgct catgggaatc aagctggcct ggatcagcag ggatgagctg    900
catcatagtc tgtgtctcca cagctataac acccaccgga atccctccga gtttcgctct    960
gccagtaaca actgacttcg cccatccttc aaatgtctcc acaaaactgt ctttgtcgaa   1020
catgcccccc aaccatttcc cttggctatc atcaatgcca ctgatggcag cacgaggatc   1080
gcatgtattc tcagggatgt aagcaacggg tctgtcaggt gggtccaaag attttgtaat   1140
aggaagaggt ccaccaatgt tggcaggaac atagctgagc cacctcaata tattagatac   1200
accttcaagg tcatcttaat taagttccat cccaatggcg cgccgagctt ggctcgagca   1260
tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga   1320
gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt   1380
gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga   1440
atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc   1500
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg   1560
gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc   1620
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc   1680
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga   1740
ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc   1800
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat   1860
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg   1920
cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc   1980
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga   2040
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact   2100
agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt   2160
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag   2220
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg   2280
tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa   2340
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata   2400
tatgagtaaa cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat   2460
ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga   2520
gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg   2580
actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt   2640
gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct   2700
ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc   2760
aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa   2820
ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca   2880
atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccagggatc   2940
gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga   3000
ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg   3060
ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag   3120
attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca   3180
tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata   3240
ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac   3300
atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa   3360
gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt   3420
atcacgaggc cctttcgtc                                                3439

<210> 17
<211> 3457
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid BAY02542 comprising donor DNA

<400> 17
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ttaattaagg tccatggcac aaggagttgg atcagcagaa gagggagttt cctctaggat    480
tcaagacttt tggcgaggcc atcccgccgc aatatgctat ccaggtactg gatgagctga    540
caaaagggga ggcgatcatt gccactggtg ttgggcagca ccagatgtgg gcggctcagt    600
attacactta caagcggcca cggcagtggc tgtcttcgtc tggtttgggg gcaatgggat    660
ttgggttacc agctgcagct ggcgctgctg tggccaaccc aggtgttaca gttgttgaca    720
ttgatggtga tggtagtttc ctcatgaaca ttcaggagtt ggcgttgatc cgcattgaga    780
acctcccagt gaaggtgatg atattgaaca accagcatct cggcatggtc gtccagctcg    840
aggataggtt ttacaaggcc aatcgggcgc acacatacct tggcaaccca gaaaatgaga    900
gtgagatata tccagatttt gtgacgattg ctaaaggatt caacgttcca gcagttcgag    960
tgacgaagaa gagcgaagtc actgcagcaa tcaagaagat gcttgagacc ccagggccat   1020
acttgttgga tatcatagtc ccgcatcagg agcacgtgct gcctatgatc ccaagcggtg   1080
gtgctttcaa ggacatgatc atggagggtg atggcaggac ctcgtactga aatttcgacc   1140
tacaagacct acaagtgtga catgcgcaat cagcatgatg cccgcgtgtt gtatcaacta   1200
ctaggggttc aactgtgagc catgcgtttt ctaggcgcgc cgttccatcc caatggcgcg   1260
ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca   1320
attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg   1380
agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg   1440
tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc   1500
tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta   1560
tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag   1620
aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg   1680
tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg   1740
tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg   1800
cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga   1860
agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc   1920
tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt   1980
aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact   2040
ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg   2100
cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt   2160
accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt   2220
ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct   2280
ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg   2340
gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt   2400
aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc   2460
atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc   2520
cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg   2580
tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca   2640
aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc   2700
aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca   2760
aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat   2820
acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac   2880
actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat   2940
gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa   3000
tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct   3060
gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc   3120
ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta   3180
tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc   3240
cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat   3300
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg   3360
cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta   3420
acctataaaa ataggcgtat cacgaggccc tttcgtc                            3457

<210> 18
<211> 6635
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid IB26 (transforming DNA)

<400> 18
gtgcaccata cctgcaggca attggccgct gtaccatgca tgatctggat tttagtactg     60
gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca    120
tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc    180
ttatctggga actactcaca cattattatg gagaaaatag agagagatag atttgtagag    240
agagactggt gatttcagcg tgtccaagct tgctagagtc gcggccgctt cagatctcgg    300
tgacgggcag gaccggacgt ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca    360
cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc    420
cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc    480
tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc    540
ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt    600
tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga    660
cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct    720
gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga    780
ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca    840
tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga    900
ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt    960
ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg   1020
cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt   1080
tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt   1140
gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca   1200
gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga   1260
agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct   1320
tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg   1380
tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt   1440
aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt   1500
ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg   1560
ttttggttta ataagaagag aaaagagttc ttttgttatg gctgaagtaa tagagaaatg   1620
agctcgagtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg   1680
atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt   1740
tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc   1800
tttgggacca ctgtcggcag gggcatcttg aacgatagcc tttcctttat cgcaatgatg   1860
gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg   1920
gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct   1980
ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac   2040
catgttgacg aagattttct tcttgtcatt gagtcgtaaa agactctgta tgaactgttc   2100
gccagtcttc acggcgagtt ctgttagatc ctcgatctga atttttgact ccatgtatgg   2160
tgcatatggc gcgccataga tccgtcaatt ctcactcatt aggcacccca ggctttacac   2220
tttatgcttc cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga   2280
aacaggatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg   2340
acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc   2400
gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa   2460
agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt   2520
tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccagatccgg   2580
cccatgatca tgtggattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg   2640
gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg   2700
ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc   2760
ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct   2820
tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa   2880
gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg   2940
gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa   3000
gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat   3060
gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg   3120
cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc   3180
atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac   3240
cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg   3300
gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc   3360
tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag   3420
cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg   3480
gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc   3540
tggagttctt cgcccacccc ctgctttaat gagatatgcg agacgcctat gatcgcatga   3600
tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc   3660
cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat   3720
gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa   3780
aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca   3840
ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg   3900
tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct   3960
agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc   4020
ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa   4080
agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc   4140
gagaattatg cagcattttt ttggtgtatg tgggccctgt acagcggccg cgttaacgcg   4200
tatactctag agcgatcgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa   4260
attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct   4320
ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc   4380
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg   4440
gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc   4500
ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag   4560
gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa   4620
aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc   4680
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc   4740
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg   4800
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt   4860
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc   4920
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc   4980
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag   5040
agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg   5100
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa   5160
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag   5220
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact   5280
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa   5340
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt   5400
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag   5460
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca   5520
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc   5580
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt   5640
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg   5700
ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca   5760
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg   5820
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca   5880
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg   5940
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct   6000
cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca   6060
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca   6120
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg   6180
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac   6240
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt   6300
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc   6360
cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat   6420
taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg   6480
gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg   6540
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc   6600
ttaactatgc ggcatcagag cagattgtac tgaga                              6635

<210> 19
<211> 19
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-048

<400> 19
atgatggcag ccctgaacg                                                  19

<210> 20
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-051

<400> 20
ggcatgttcg acaaagacag                                                 20

<210> 21
<211> 19
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-053

<400> 21
caactgcatg tgggagctg                                                  19

<210> 22
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-054

<400> 22
acaattgttg acccagcctg                                                 20

<210> 23
<211> 23
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-065

<400> 23
gttacagttg ttgacattga tgg                                             23

<210> 24
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-066

<400> 24
aagtatggcc ctggggtctc                                                 20

<210> 25
<211> 23
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-101

<400> 25
ctattgccag tgcctattct agg                                             23

<210> 26
<211> 21
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-102

<400> 26
gtgacgctgt ccgggaacca c                                               21

<210> 27
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-110

<400> 27
ctgcttgcca cttacacctc                                                 20

<210> 28
<211> 19
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-111

<400> 28
gttgggcgtc gagaacctc                                                  19

<210> 29
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-112

<400> 29
acttgcccag cacgaggaac                                                 20

<210> 30
<211> 19
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-113

<400> 30
gctaggtgtg gagaacctc                                                  19

<210> 31
<211> 21
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-130

<400> 31
gatgcatggc actgtgtatg c                                               21

<210> 32
<211> 18
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-135

<400> 32
ggcatggtcg tccagctc                                                   18

<210> 33
<211> 21
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-136

<400> 33
cagaaaggaa ccagcacact g                                               21

<210> 34
<211> 18
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-18-162

<400> 34
gttccttccc cttgcatc                                                   18

<210> 35
<211> 3496
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bas04096 comprising donor DNA

<400> 35
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggctt    420
ttggcggaat gatcaattgc cctcgtttat tgcaccgctg atttccggtg ggcatattaa    480
ccgatcttgg acatgtccac tcctgaatat cccaatggca ggcagattgt tgtcatcgca    540
aatgatatta cttttagagc tggatcgttt ggtccaaggg aagatgcatt ttttgaaact    600
gttaccaacc tagcttgtga gaggaagctt cctctcatct acttggcagc aaactctggt    660
gctcggatcg gcatagcaga tgaagtaaaa tcttgcttcc gtgttggatg gtctgatgat    720
ggcagccctg aacgtgggtt tcagtacatt tatctgactg aagaagatca tgctcgtatt    780
agcacttctg ttatagcgca caagatgcag cttgataatg gtgaaatccg ctgggttatc    840
gattctgttg tggggaagga ggatgggcta ggtgtggaga acctccatgg aagtgctgct    900
attgccagtg cctattctag ggcctatgag gagacattta cgcttacatt tgtgactgga    960
cggactgttg gaataggagc atatcttgct cgacttggca tacggtgcat acagcgtact   1020
gatcagccca ttatcctaac tgggttctct gccttgaaca agcttcttgg ccgggaagtt   1080
tacagctccc acatgcagtt gggtggcccc aaaattatgg cgacaaacgg tgttgtccat   1140
ctgacagttt cagatgacct tgaaggtgta tctaatatat tgaggtggct cagctatgtt   1200
cctgccaaca ttggtggacc tcttcctatt acaaaatctt tggacccacc tgacagaccc   1260
gttgcttaca tccctgagaa tacatatccc aatggcgcgc cgagcttggc tcgagcatgg   1320
tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc   1380
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg   1440
ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc   1500
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact   1560
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta   1620
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag   1680
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc   1740
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta   1800
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg   1860
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc   1920
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac   1980
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac   2040
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   2100
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   2160
agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   2220
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   2280
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   2340
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   2400
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   2460
gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta   2520
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa   2580
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact   2640
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag   2700
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc   2760
cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa   2820
ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga   2880
caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata   2940
ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc agggatcgca   3000
gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc   3060
ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta   3120
cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt   3180
gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc   3240
atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcatactc   3300
ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata   3360
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg   3420
ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc   3480
acgaggccct ttcgtc                                                   3496

<210> 36
<211> 3490
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bay02544 comprising donor DNA

<400> 36
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatccaag    420
ctcggcgcgc cattgggatg gcgcgccatg tattctcagg gatgtaagca acgggtctgt    480
caggtgggtc caaagatttt gtaataggaa gaggtccacc aatgttggca ggaacatagc    540
tgagccacct caatatatta gatacacctt caaggtcatc tgaaactgtc agatggacaa    600
caccgtttgt cgccataatt ttggggccac ccaactgcat gtgggagctg taaacttccc    660
ggccaagaag cttgttcaag gcagagaacc cagttaggat aatgggctgg tcagtacgct    720
gtatgcaccg tatgccaagt cgagcaagat atgctcctat tccaacagtc cgtccagtca    780
caaatgtaag cgtaaatgtc tcctcatagg cgcggctata cgcactcgca atagcaacac    840
ttccatggag gttctcgacg cccaacccat cctccttccc cacaacagaa tcgataaccc    900
acctaatttc accattatca agctgcatct tgtgcgctat aacagaagtg ctaatacgag    960
catggtcttc ttcagtcaga taaatgtact gaaacccacg ttcagggctg ccatcatcag   1020
accatccaac acggaagcaa gattttactt catctgctat gccgatccga gcaccagagt   1080
ttgctgccaa gtagatgaga ggaagcttcc tctcacaagc taggttggta acagtttcaa   1140
aaaatgcatc ttcccttgga ccaaacgatc cagctctaaa agtaatatca tttgcgatga   1200
caacaatctg cctgccattg ggatattcag gagtggacat gtccaagatc ttaattaaat   1260
cccaatacgc gtcaattcaa tcccaatggc gcgccgagct tggctcgagc atggtcatag   1320
ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc   1380
ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc   1440
tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa   1500
cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg   1560
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg   1620
ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag   1680
gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac   1740
gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga   1800
taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt   1860
accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc   1920
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc   1980
cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta   2040
agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat   2100
gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca   2160
gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct   2220
tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt   2280
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct   2340
cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc   2400
acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa   2460
acttggtctg acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata   2520
tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca   2580
ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca   2640
acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca   2700
ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact   2760
tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta   2820
ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta   2880
caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca   2940
cctgaatcag gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg   3000
agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat   3060
tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg   3120
ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca   3180
cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg   3240
gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt   3300
tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   3360
tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   3420
gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg   3480
ccctttcgtc                                                          3490

<210> 37
<211> 3155
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bas04093 comprising donor DNA

<400> 37
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgatga    420
gaccgaggtc gtgatgtctg aagtacgcca atacatctat ggcatcggta cgcagtgacc    480
aagcccgtta ttctgacagt tctggtgctc aacacattta tatttatcaa ggagcacatt    540
gttactcact gctaggaggg aatcgaacta ggaatattga tcagaggaac tacgagagag    600
ctgaagataa ctgccctcta gctctcactg atctgggtcg catagtgaga tgcagcccac    660
gtgagttcag caacggtcta gcgctgggct tttaggcccg catgatcggg cttttgtcgg    720
gtggtcgacg tgttcacgat tggggagagc aacgcagcag ttcctcttag tttagtccca    780
cctcgcctgt ccagcagagt tctgaccggt ttataaactc gcttgctgca tcagacttga    840
cccagttagg ataatgggcg ttttagagct agaaatagca agttaaaata aggctagtcc    900
gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttttatccca atggcgcgcc    960
gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat   1020
tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag   1080
ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg   1140
ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc   1200
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc   1260
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa   1320
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt   1380
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg   1440
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg   1500
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag   1560
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc   1620
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa   1680
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg   1740
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc   1800
taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac   1860
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg   1920
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt   1980
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt   2040
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa   2100
atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat   2160
caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg   2220
tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta   2280
tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa   2340
aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa   2400
aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa   2460
atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac   2520
gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac   2580
tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc   2640
tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg   2700
cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt   2760
aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt   2820
cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata   2880
cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg   2940
ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg   3000
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   3060
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   3120
ctataaaaat aggcgtatca cgaggccctt tcgtc                              3155

<210> 38
<211> 8853
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bas03568 encoding a modified LbCas12a

<400> 38
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gggcccggcc ggccgcgatc    420
gcgcggccgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat    480
tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc    540
agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt    600
actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa    660
ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt    720
tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca    780
tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt    840
attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat    900
aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag    960
aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa   1020
acgccgtcga tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg   1080
ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc   1140
cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag   1200
acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg   1260
attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc   1320
cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat   1380
ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc   1440
cccccctctc taccttctct agatcggcgt tccggtccat gcttagggcc cggtagttct   1500
acttctgtcc atgtttgtgt tagatccgtg tttgtgttag atccgtgcta ctagcgttcg   1560
tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct   1620
ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt   1680
ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac   1740
ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg   1800
ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta   1860
attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat   1920
ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag   1980
agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt   2040
tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg   2100
tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta   2160
ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc   2220
atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat   2280
aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt   2340
tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca   2400
ccctgttgtt tggtgttact tctgcagcca tggcgcccaa gaagaagagg aaggtgggca   2460
tccacggcgt ccccgccgcc atgagcaagc tggagaagtt caccaactgc tactcactct   2520
ccaagaccct ccgcttcaag gccatccccg tcggcaagac ccaggagaac atcgacaaca   2580
agaggctgct ggtggaggat gagaagaggg ccgaggacta caagggcgtc aagaagctcc   2640
tcgaccggta ctacctctca ttcatcaatg atgtcctcca ctccatcaag ctgaagaacc   2700
tcaacaacta catctccctc ttccgcaaga agacaaggac cgagaaggag aacaaggagc   2760
tggagaacct ggagatcaac ctccgcaagg agatcgccaa ggccttcaag ggcaacgagg   2820
gctacaagag cctcttcaag aaggacatca tcgagacgat cctaccggag ttcctggatg   2880
acaaggacga gatcgccctg gtgaacagct tcaacggctt caccaccgcc ttcaccggct   2940
tcttcgacaa ccgggagaac atgttctccg aggaggccaa gagcacctcc atcgccttca   3000
ggtgcatcaa cgagaacctc acccgctaca tctcaaacat ggacatcttc gagaaggtgg   3060
acgccatctt cgacaagcat gaggtccagg agatcaagga gaagatcctg aactccgact   3120
acgacgtgga ggacttcttc gagggcgagt tcttcaactt cgtcctgacc caagaaggca   3180
tcgacgtcta caacgccatc atcggcggct tcgtcaccga gtcaggtgag aagatcaagg   3240
gcctcaacga gtacatcaac ctctacaacc agaagaccaa gcagaagctc cccaagttca   3300
agcccctcta caagcaggtg ctctccgacc gtgagagctt gagcttctac ggcgagggct   3360
acacctccga cgaggaggtg ctggaggtct tccgcaacac cctcaacaag aactcagaga   3420
tcttctcctc catcaagaag ctggagaagc tcttcaagaa cttcgacgag tactcctccg   3480
ccggcatctt cgtgaagaac ggcccggcca tctcaaccat ctccaaggac atcttcggcg   3540
agtggaacgt catccgtgac aagtggaacg ccgagtacga cgacatccac ctcaagaaga   3600
aggccgtggt gaccgagaag tacgaggacg accgccgcaa gagcttcaag aagatcggca   3660
gcttctcact ggagcagctc caggagtacg ccgacgccga cctctccgtg gtggagaagc   3720
tgaaggagat catcatccag aaggtggacg agatctacaa ggtgtacggc agctctgaga   3780
agctcttcga cgccgacttc gtcctggaga agagcctcaa gaagaacgac gccgtggtgg   3840
ccatcatgaa ggacctcctc gactcagtca agagcttcga gaactacatc aaggccttct   3900
tcggcgaggg caaggagaca aaccgcgacg agagcttcta cggcgacttc gtcctcgcct   3960
atgacatcct cctcaaggtg gaccacatct acgacgccat caggaactac gtcacccaga   4020
agccctactc aaaggacaag ttcaagctct acttccagaa cccccagttc atgaggggct   4080
gggacaagga caaggagact gactaccgcg ccaccatcct ccgctacggc tcaaagtact   4140
acctcgccat catggacaag aagtacgcca agtgcctcca gaagatcgac aaggatgatg   4200
tgaacggcaa ctacgagaag atcaactaca agctcctccc cggccccaac aagatgctac   4260
caagggtctt cttctccaag aagtggatgg cctactacaa cccctccgag gacatccaga   4320
agatctacaa gaacggcacc ttcaagaagg gtgacatgtt caacctcaac gactgccaca   4380
agctgatcga cttcttcaag gacagcatct caaggtaccc caagtggagc aacgcctacg   4440
acttcaactt ctctgagacg gagaagtaca aggacatcgc cggcttctac cgggaggtgg   4500
aggagcaagg ctacaaggtg agcttcgagt ccgcctccaa gaaggaggtg gacaagctgg   4560
tggaggaggg caagctctac atgttccaga tctacaacaa ggacttctct gacaagagcc   4620
acggcacccc caacctacac accatgtact tcaagctcct cttcgacgag aacaaccacg   4680
gccagatccg cctctccggc ggcgccgagc tcttcatgag gagggcctcc ctcaagaagg   4740
aggagctggt ggttcacccg gccaacagcc ccatcgccaa caagaaccct gacaacccca   4800
agaagaccac caccctctcc tacgacgtct acaaggacaa gaggttctca gaagaccagt   4860
acgagctcca catccccatc gccatcaaca agtgccccaa gaacatcttc aagatcaaca   4920
ccgaggtgag ggtgctactc aagcatgatg ataaccccta cgtcatcggc atcgaccgcg   4980
gcgagaggaa cctcctctac atcgtggtgg tggacggcaa gggcaacatc gtggagcagt   5040
actccttgaa cgagatcatc aacaacttca acggcatcag gatcaagacc gactaccact   5100
cactccttga caagaaggag aaggagaggt tcgaggcccg gcaaaattgg acaagcatcg   5160
agaacatcaa ggagctgaag gccggctaca tctcccaggt ggtgcacaag atctgtgagc   5220
tggtggagaa gtacgacgcc gtcatcgccc tggaggacct caacagcggc ttcaagaact   5280
caagggtcaa ggtggagaag caggtgtacc agaagttcga gaagatgctc atcgacaagc   5340
tgaactacat ggtggacaag aagagcaacc cctgcgccac cggcggcgcc ctcaagggct   5400
accagatcac caacaagttt gagagcttca agagcatgtc aacccagaac ggcttcatct   5460
tctacatccc ggcctggctc acctccaaga tcgacccctc caccggcttc gtcaacctcc   5520
tcaagaccaa gtacacctcc atagccgaca gcaagaagtt catctcctcc ttcgaccgca   5580
tcatgtacgt ccctgaggag gacctcttcg agttcgccct cgactacaag aacttctcaa   5640
ggaccgacgc cgactacatc aagaagtgga agctctacag ctacggcaac cgcatcagga   5700
tcttcaggaa ccccaagaag aacaacgtct tcgactggga ggaggtgtgc ctgacctccg   5760
cctacaagga gctcttcaac aagtacggca tcaactacca gcaaggtgac atcagggcgc   5820
tcctatgcga gcagagcgac aaggccttct actcctcctt catggccctc atgagcttga   5880
tgctccagat gaggaacagc atcaccggcc gcaccgacgt cgacttcctc atctcccccg   5940
tcaagaactc tgatggcatc ttctacgact caaggaacta cgaggcccag gagaacgcca   6000
tcctccccaa gaacgccgac gccaacggcg cctacaacat cgccaggaag gtgctctggg   6060
ccatcggcca gttcaagaag gccgaggatg agaagctgga caaggtgaag atcgccatct   6120
caaacaagga gtggctggag tacgcccaga cctccgtcaa gcacaagagg ccggcggcca   6180
ccaagaaggc cggccaggcc aagaagaaga agggcagcta cccctacgac gtccccgact   6240
acgcctaccc ctacgacgtc cccgactacg cctaccccta cgacgtcccc gactacgcct   6300
aattgctagc acgcgtcgaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat   6360
tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc   6420
atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag   6480
tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata   6540
aattatcgcg cgcggtgtca tctatgttac tagatccctg caggcccggg ttaattaaat   6600
ttaaatggcg cgccagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt   6660
atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg   6720
cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg   6780
gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc   6840
gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc   6900
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata   6960
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg   7020
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct   7080
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa   7140
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc   7200
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt   7260
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg   7320
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg   7380
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct   7440
tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc   7500
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg   7560
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc   7620
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt   7680
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa   7740
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat   7800
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct   7860
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg   7920
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag   7980
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta   8040
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg   8100
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg   8160
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct   8220
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta   8280
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg   8340
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc   8400
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg   8460
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga   8520
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg   8580
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat   8640
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc   8700
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca   8760
catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct   8820
ataaaaatag gcgtatcacg aggccctttc gtc                                8853

<210> 39
<211> 1290
<212> PRT
<213> Artificial sequence

<220>
<223> Modified LbCas12a from Plasmid Bas03568

<400> 39
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1               5                   10                  15
Ala Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys
            20                  25                  30
Thr Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile
        35                  40                  45
Asp Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr
    50                  55                  60
Lys Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn
65                  70                  75                  80
Asp Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser
                85                  90                  95
Leu Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu
            100                 105                 110
Asn Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly
        115                 120                 125
Asn Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile
    130                 135                 140
Leu Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser
145                 150                 155                 160
Phe Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu
                165                 170                 175
Asn Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys
            180                 185                 190
Ile Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu
        195                 200                 205
Lys Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu
    210                 215                 220
Lys Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu
225                 230                 235                 240
Phe Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala
                245                 250                 255
Ile Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu
            260                 265                 270
Asn Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro
        275                 280                 285
Lys Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu
    290                 295                 300
Ser Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val
305                 310                 315                 320
Phe Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys
                325                 330                 335
Lys Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly
            340                 345                 350
Ile Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile
        355                 360                 365
Phe Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp
    370                 375                 380
Asp Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp
385                 390                 395                 400
Asp Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln
                405                 410                 415
Leu Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys
            420                 425                 430
Glu Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser
        435                 440                 445
Ser Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys
    450                 455                 460
Lys Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val
465                 470                 475                 480
Lys Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu
                485                 490                 495
Thr Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp
            500                 505                 510
Ile Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val
        515                 520                 525
Thr Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn
    530                 535                 540
Pro Gln Phe Met Arg Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg
545                 550                 555                 560
Ala Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp
                565                 570                 575
Lys Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn
            580                 585                 590
Gly Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys
        595                 600                 605
Met Leu Pro Arg Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn
    610                 615                 620
Pro Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys
625                 630                 635                 640
Gly Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe
                645                 650                 655
Lys Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe
            660                 665                 670
Asn Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg
        675                 680                 685
Glu Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys
    690                 695                 700
Lys Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln
705                 710                 715                 720
Ile Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu
                725                 730                 735
His Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln
            740                 745                 750
Ile Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu
        755                 760                 765
Lys Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn
    770                 775                 780
Lys Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val
785                 790                 795                 800
Tyr Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro
                805                 810                 815
Ile Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu
            820                 825                 830
Val Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile
        835                 840                 845
Asp Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys
    850                 855                 860
Gly Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe
865                 870                 875                 880
Asn Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys
                885                 890                 895
Glu Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn
            900                 905                 910
Ile Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile
        915                 920                 925
Cys Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu
    930                 935                 940
Asn Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr
945                 950                 955                 960
Gln Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp
                965                 970                 975
Lys Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln
            980                 985                 990
Ile Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly
        995                 1000                1005
Phe Ile Phe Tyr Ile Pro Ala Trp Leu Thr Ser Lys Ile Asp Pro Ser
    1010                1015                1020
Thr Gly Phe Val Asn Leu Leu Lys Thr Lys Tyr Thr Ser Ile Ala Asp
1025                1030                1035                1040
Ser Lys Lys Phe Ile Ser Ser Phe Asp Arg Ile Met Tyr Val Pro Glu
                1045                1050                1055
Glu Asp Leu Phe Glu Phe Ala Leu Asp Tyr Lys Asn Phe Ser Arg Thr
            1060                1065                1070
Asp Ala Asp Tyr Ile Lys Lys Trp Lys Leu Tyr Ser Tyr Gly Asn Arg
        1075                1080                1085
Ile Arg Ile Phe Arg Asn Pro Lys Lys Asn Asn Val Phe Asp Trp Glu
    1090                1095                1100
Glu Val Cys Leu Thr Ser Ala Tyr Lys Glu Leu Phe Asn Lys Tyr Gly
1105                1110                1115                1120
Ile Asn Tyr Gln Gln Gly Asp Ile Arg Ala Leu Leu Cys Glu Gln Ser
                1125                1130                1135
Asp Lys Ala Phe Tyr Ser Ser Phe Met Ala Leu Met Ser Leu Met Leu
            1140                1145                1150
Gln Met Arg Asn Ser Ile Thr Gly Arg Thr Asp Val Asp Phe Leu Ile
        1155                1160                1165
Ser Pro Val Lys Asn Ser Asp Gly Ile Phe Tyr Asp Ser Arg Asn Tyr
    1170                1175                1180
Glu Ala Gln Glu Asn Ala Ile Leu Pro Lys Asn Ala Asp Ala Asn Gly
1185                1190                1195                1200
Ala Tyr Asn Ile Ala Arg Lys Val Leu Trp Ala Ile Gly Gln Phe Lys
                1205                1210                1215
Lys Ala Glu Asp Glu Lys Leu Asp Lys Val Lys Ile Ala Ile Ser Asn
            1220                1225                1230
Lys Glu Trp Leu Glu Tyr Ala Gln Thr Ser Val Lys His Lys Arg Pro
        1235                1240                1245
Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Gly Ser Tyr
    1250                1255                1260
Pro Tyr Asp Val Pro Asp Tyr Ala Tyr Pro Tyr Asp Val Pro Asp Tyr
1265                1270                1275                1280
Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala
                1285                1290

<210> 40
<211> 20
<212> DNA
<213> Artificial sequence

<220>
<223> Primer HT-19-022

<400> 40
ggctaggagt cgaaaacctc                                                 20

<210> 41
<211> 2918
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bas03609 comprising guide RNA

<400> 41
gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg     60
agagaaaccc aattgggtac cgagctccct gcaggaagct taattaagat gagaccgagg    120
tcgtgatgtc tgaagtacgc caatacatct atggcatcgg tacgcagtga ccaagcccgt    180
tattctgaca gttctggtgc tcaacacatt tatatttatc aaggagcaca ttgttactca    240
ctgctaggag ggaatcgaac taggaatatt gatcagagga actacgagag agctgaagat    300
aactgccctc tagctctcac tgatctgggt cgcatagtga gatgcagccc acgtgagttc    360
agcaacggtc tagcgctggg cttttaggcc cgcatgatcg ggcttttgtc gggtggtcga    420
cgtgttcacg attggggaga gcaacgcagc agttcctctt agtttagtcc cacctcgcct    480
gtccagcaga gttctgaccg gtttataaac tcgcttgctg catcagactt gtaatttcta    540
ctaagtgtag atcacctagc ccatcctcct tcccctttta ttttttggcg cgccacctgc    600
tagcgctcgc ttggatccga attcaaacct gaaattgtta tccgctcaca attccacaca    660
acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca    720
cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc    780
attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt    840
cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact    900
caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag    960
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata   1020
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc   1080
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg   1140
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc   1200
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg   1260
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc   1320
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga   1380
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg   1440
gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa   1500
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg   1560
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt   1620
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat   1680
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct   1740
aaagtatata tgagtaaact tggtctgaca gccggaattg ccagctgggg cgccctctgg   1800
taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg   1860
gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca   1920
agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg   1980
ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg   2040
cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc   2100
agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt   2160
cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc   2220
atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca   2280
tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc   2340
acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg   2400
gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct   2460
cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc   2520
tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc   2580
tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta   2640
cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt   2700
ctgatccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca   2760
tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg   2820
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag   2880
cggatgccgg gagcagacaa gcccgtcagg gcgcgtca                           2918

<210> 42
<211> 3455
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid Bas03253 comprising donor DNA

<400> 42
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
ggcgcgccat gtattctcag ggatgtaagc aacgggtctg tcaggtgggt ccaaagattt    480
tgtaatagga agaggtccac caatgttggc aggaacatag ctgagccacc tcaatatatt    540
agatacacct tcaaggtcat ctgaaactgt cagatggaca acaccgtttg tcgccataat    600
tttggggcca cccaactgca tgtgggagct gtaaacttcc cggccaagaa gcttgttcaa    660
ggcagagaac ccagttagga taatgggctg gtcagtacgc tgtatgcacc gtatgccaag    720
tcgagcaaga tatgctccta ttccaacagt ccgtccagtc acaaatgtaa gcgtaaatgt    780
ctcctcatag gccctagaat aggcactggc aatagcagca cttccatgga ggttttcgac    840
tcctagccca tcctccttcc ccacaacaga atcgataacc cacctaattt caccattatc    900
aagctgcatc ttgtgcgcta taacagaagt gctaatacga gcatggtctt cttcagtcag    960
ataaatgtac tgaaacccac gttcagggct gccatcatca gaccatccaa cacggaagca   1020
agattttact tcatctgcta tgccgatccg agcaccagag tttgctgcca agtagatgag   1080
aggaagcttc ctctcacaag ctaggttggt aacagtttca aaaaatgcat cttcccttgg   1140
accaaacgat ccagctctaa aagtaatatc atttgcgatg acaacaatct gcctgccatt   1200
gggatattca ggagtggaca tgtccaagat cttaattaag ttccatccca atggcgcgcc   1260
gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat   1320
tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag   1380
ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg   1440
ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc   1500
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc   1560
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa   1620
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt   1680
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg   1740
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg   1800
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag   1860
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc   1920
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa   1980
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg   2040
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc   2100
taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac   2160
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg   2220
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt   2280
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt   2340
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa   2400
atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat   2460
caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg   2520
tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta   2580
tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa   2640
aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa   2700
aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa   2760
atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac   2820
gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac   2880
tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc   2940
tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg   3000
cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt   3060
aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt   3120
cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata   3180
cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg   3240
ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg   3300
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg   3360
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac   3420
ctataaaaat aggcgtatca cgaggccctt tcgtc                              3455

<210> 43
<211> 9186
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA824 encoding Cas9 D10A nickase

<400> 43
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gggcccggcc ggccgcgatc    420
gcgcggccgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat    480
tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc    540
agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt    600
actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa    660
ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt    720
tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca    780
tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt    840
attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat    900
aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag    960
aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa   1020
acgccgtcga tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg   1080
ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc   1140
cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag   1200
acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg   1260
attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc   1320
cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat   1380
ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc   1440
cccccctctc taccttctct agatcggcgt tccggtccat gcttagggcc cggtagttct   1500
acttctgtcc atgtttgtgt tagatccgtg tttgtgttag atccgtgcta ctagcgttcg   1560
tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct   1620
ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt   1680
ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac   1740
ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg   1800
ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta   1860
attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat   1920
ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag   1980
agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt   2040
tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg   2100
tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta   2160
ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc   2220
atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat   2280
aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt   2340
tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca   2400
ccctgttgtt tggtgttact tctgcagcca tggcccctaa gaagaaacga aaggtaggca   2460
tccacggtgt tccagcggct atggacaaga agtacagcat cgggctggcc attggcacga   2520
actctgttgg gtgggcggtt atcactgacg agtacaaggt gccgtcgaag aagttcaagg   2580
tgctaggcaa caccgaccgt cacagcatca agaagaacct catcggcgcc ctgttgtttg   2640
actctgggga aaccgccgag gctactcgac taaagagaac cgcccgtagg cgatacacta   2700
ggcggaagaa caggatctgt tatctccagg agatcttcag caacgagatg gcgaaggtcg   2760
atgactcatt cttccaccgc cttgaggagt ctttcctggt cgaggaggac aaaaagcacg   2820
agcgacaccc gatcttcggg aacatcgttg atgaggtagc ctaccacgag aagtacccta   2880
ctatctacca cctgcgcaag aaactagtgg actccaccga caaggcggac ctccgcctta   2940
tctatctcgc cctcgcccac atgatcaagt ttcgcgggca cttcctcatc gaaggcgatc   3000
tcaatccgga caacagcgac gtggacaagc tattcatcca gctagttcag acctacaacc   3060
agctctttga agagaaccct attaacgcga gcggggtgga cgccaaagcc atcctttccg   3120
ccaggctctc taagagccgg agactcgaga accttatcgc tcagttgccc ggcgagaaga   3180
aaaacggcct tttcggcaac ctgattgccc tgtccttggg cttgacgccg aacttcaaga   3240
gcaacttcga cctagctgag gatgctaaac tgcagctgtc caaggacact tatgatgacg   3300
accttgacaa cctcctggcc caaattgggg atcagtacgc ggacctcttc ctcgctgcca   3360
agaacctctc cgacgcgatt ctgttgtcgg acatcctccg cgtgaacacc gagattacta   3420
aggccccact gtccgcgagc atgatcaaac gctacgacga gcaccatcag gatctaactc   3480
tgctcaaggc gctcgtccgt cagcaactcc ctgagaagta caaggagatc ttcttcgacc   3540
agtccaagaa cggctacgcc ggttacattg atggtggcgc gagccaggag gagttctaca   3600
agttcatcaa gcccatctta gagaagatgg acggcaccga agagcttctc gtgaagctca   3660
acagggagga cctcctaagg aagcagcgca cctttgacaa cgggtctatt cctcatcaga   3720
tccatctcgg cgagctccac gcgattttga gacgccaaga ggacttctac ccgttcttga   3780
aggacaaccg ggagaagatc gagaagatcc tcacgttcag gatcccttat tacgtcggcc   3840
ccctagctag gggtaactcg agattcgcgt ggatgaccag aaagtccgag gaaaccatta   3900
ccccgtggaa ctttgaagag gtggtcgaca agggcgcgag cgcgcaatcg ttcattgagc   3960
ggatgaccaa cttcgacaag aacctaccga acgagaaggt cctgccgaaa cactccctgc   4020
tctacgagta cttcaccgtt tataacgagc tgacgaaggt caagtacgtg actgagggca   4080
tgcgtaaacc agcgttcctc agcggtgagc agaagaaggc tatcgtggac ctgctcttta   4140
agacgaaccg caaggtgacc gtgaagcagc tcaaggagga ttatttcaag aagatcgagt   4200
gcttcgactc agtggagatt agcggcgtgg aggaccgttt caatgcgagc ttaggtacct   4260
accacgacct cctgaagatc atcaaggaca aggacttcct cgacaacgag gaaaacgagg   4320
acatcctgga ggacatcgtg ctcaccctga ccctctttga agacagggag atgatcgagg   4380
aaaggttgaa gacctacgcg cacctattcg acgacaaggt catgaaacag ctcaagcgga   4440
ggagatacac tggctggggc agactttcgc ggaagctcat caatgggatc agggacaagc   4500
agtccggcaa gaccatcctg gacttcttga agagcgacgg cttcgctaat cgcaacttca   4560
tgcagctcat tcatgacgac tccctaacat tcaaggagga catccaaaag gcgcaagtct   4620
cgggtcaggg ggattctttg cacgagcaca tcgcaaacct cgccggttct ccggccatta   4680
aaaagggcat ccttcagacg gtcaaggtgg tcgacgagct cgtgaaggtt atgggccggc   4740
ataagcccga gaacatagtg atcgagatgg ctagggagaa ccagaccacg caaaagggcc   4800
agaagaactc acgtgagcgc atgaagagga tcgaggaagg tatcaaggag ctgggctctc   4860
agattctcaa ggagcatccg gtcgagaaca cccagcttca gaacgagaag ctgtacctct   4920
actacctcca gaatggccgc gacatgtacg ttgatcagga gttagatatt aatcggctga   4980
gcgactacga cgttgaccac atcgtgcccc agtcgttcct caaggatgac tccatcgaca   5040
acaaggtcct gaccagatcg gacaagaacc gtggtaagag cgacaatgtg ccctccgagg   5100
aggtggtcaa aaagatgaag aactattggc ggcagttgct gaacgccaag ctcatcaccc   5160
agcgaaagtt cgataattta acgaaggccg aacgaggggg cctttccgaa ctcgataagg   5220
ccggtttcat caagcgtcag ctggttgaga cgcgacagat cacgaagcat gtggcccaga   5280
ttctggacag ccggatgaac acgaagtacg acgagaacga caaacttatt cgggaggtga   5340
aggtgataac cctcaagtcc aagttagtgt cggacttccg caaggacttt cagttctata   5400
aagtgcgcga gatcaacaat taccaccacg cccacgacgc ctacttgaat gccgtggtcg   5460
gcacggcgct catcaaaaag taccccaagc tggagagcga gttcgtctac ggcgactaca   5520
aggtttatga cgtcaggaag atgattgcga agtcagagca agagatcggg aaggccaccg   5580
ccaagtactt cttctactcg aacatcatga acttctttaa gaccgagatc accctcgcca   5640
atggggagat ccggaaacgt ccccttatcg agacgaacgg cgagactggc gagatagtct   5700
gggacaaagg tcgcgatttc gccaccgttc gaaaggtgct ctcaatgcct caggtcaata   5760
tcgtgaagaa gaccgaggtt cagacggggg gcttcagcaa ggagtccatc ctccctaagc   5820
gcaactctga caagctgatc gcccggaaaa aggattggga cccgaaaaag tacggcggct   5880
tcgattctcc caccgtagct tatagcgtgc tcgtggttgc caaggtggag aagggcaaga   5940
gcaagaaact gaagtcggtc aaggagttac tcggcatcac aatcatggag cgcagctcgt   6000
ttgaaaagaa cccgatcgac ttcctggagg ccaagggcta taaggaggtg aagaaggacc   6060
tcatcattaa gctgccgaag tacagcttgt tcgagctcga gaatggcagg aagaggatgc   6120
tagcttcggc cggcgaactt caaaaaggga acgagctcgc gttgccgtcg aagtacgtga   6180
acttcctcta cttagctagt cactatgaaa agctgaaggg ctcgccggag gacaacgagc   6240
agaagcagct cttcgtcgag caacacaagc actacctcga cgagatcatc gagcagataa   6300
gtgagttcag caagcgcgtg atcctcgctg atgccaacct cgataaggtg ctcagcgcct   6360
acaataagca ccgagacaag ccgattcggg agcaggcgga gaacatcatt catttattta   6420
cgctcactaa cctaggcgct ccggctgcct tcaagtactt cgacaccacg atcgacagga   6480
agcgctacac cagcaccaaa gaagtcctcg acgcgacgct cattcaccag agcatcacag   6540
ggctctacga gactcggatc gacttgtcgc aattgggtgg ggataagagg ccggccgcga   6600
ctaaaaaggc cggccaagcc aagaaaaaga aatgattgct agcacgcgtc gaagcagatc   6660
gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga   6720
ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga   6780
cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga   6840
tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt   6900
tactagatcc ctgcaggccc gggttaatta aatttaaatg gcgcgccagc ttggcgtaat   6960
catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac   7020
gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa   7080
ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat   7140
gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc   7200
tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg   7260
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag   7320
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc   7380
gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag   7440
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga   7500
ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc   7560
atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg   7620
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt   7680
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca   7740
gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca   7800
ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag   7860
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca   7920
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg   7980
ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa   8040
aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta   8100
tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag   8160
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga   8220
tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac   8280
cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc   8340
ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta   8400
gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac   8460
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat   8520
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa   8580
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg   8640
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag   8700
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc   8760
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct   8820
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat   8880
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg   8940
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc   9000
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta   9060
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg   9120
tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct   9180
ttcgtc                                                              9186

<210> 44
<211> 1401
<212> PRT
<213> Artificial sequence

<220>
<223> Cas9 D10A nickase from Plasmid KVA824

<400> 44
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1               5                   10                  15
Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser
            20                  25                  30
Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys
        35                  40                  45
Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu
    50                  55                  60
Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg
65                  70                  75                  80
Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile
                85                  90                  95
Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp
            100                 105                 110
Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys
        115                 120                 125
Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala
    130                 135                 140
Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val
145                 150                 155                 160
Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala
                165                 170                 175
His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn
            180                 185                 190
Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr
        195                 200                 205
Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp
    210                 215                 220
Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu
225                 230                 235                 240
Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly
                245                 250                 255
Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn
            260                 265                 270
Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr
        275                 280                 285
Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala
    290                 295                 300
Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser
305                 310                 315                 320
Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala
                325                 330                 335
Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu
            340                 345                 350
Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe
        355                 360                 365
Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala
    370                 375                 380
Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met
385                 390                 395                 400
Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu
                405                 410                 415
Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His
            420                 425                 430
Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro
        435                 440                 445
Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg
    450                 455                 460
Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala
465                 470                 475                 480
Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu
                485                 490                 495
Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met
            500                 505                 510
Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His
        515                 520                 525
Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val
    530                 535                 540
Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu
545                 550                 555                 560
Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val
                565                 570                 575
Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe
            580                 585                 590
Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
        595                 600                 605
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu
    610                 615                 620
Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu
625                 630                 635                 640
Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr
                645                 650                 655
Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg
            660                 665                 670
Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg
        675                 680                 685
Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly
    690                 695                 700
Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr
705                 710                 715                 720
Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser
                725                 730                 735
Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys
            740                 745                 750
Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met
        755                 760                 765
Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn
    770                 775                 780
Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg
785                 790                 795                 800
Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His
                805                 810                 815
Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr
            820                 825                 830
Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
        835                 840                 845
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu
    850                 855                 860
Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn
865                 870                 875                 880
Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met
                885                 890                 895
Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg
            900                 905                 910
Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu
        915                 920                 925
Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
    930                 935                 940
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr
945                 950                 955                 960
Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys
                965                 970                 975
Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val
            980                 985                 990
Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala
        995                 1000                1005
Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu
    1010                1015                1020
Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala
1025                1030                1035                1040
Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr
                1045                1050                1055
Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly
            1060                1065                1070
Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
        1075                1080                1085
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu
    1090                1095                1100
Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly
1105                1110                1115                1120
Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu
                1125                1130                1135
Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp
            1140                1145                1150
Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys
        1155                1160                1165
Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
    1170                1175                1180
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu
1185                1190                1195                1200
Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro
                1205                1210                1215
Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala
            1220                1225                1230
Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys
        1235                1240                1245
Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly
    1250                1255                1260
Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys
1265                1270                1275                1280
His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg
                1285                1290                1295
Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn
            1300                1305                1310
Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
        1315                1320                1325
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe
    1330                1335                1340
Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu
1345                1350                1355                1360
Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg
                1365                1370                1375
Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala Thr Lys
            1380                1385                1390
Lys Ala Gly Gln Ala Lys Lys Lys Lys
        1395                1400

<210> 45
<211> 3318
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA766 comprising guide RNA

<400> 45
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac    420
cctgcaggaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc    480
cttattttaa cttgctattt ctagctctaa aacttgacag cagccgtgac tgcaagctga    540
ctacagcgcg cgggtttata agctggttcc atcgtctttc ggtttccgat gtggtactaa    600
acatcccccg caacgccacc gcgcaaacca tcggatcccc atccaacgga ctaaagtggg    660
tagcagcgcc acccgcgcgg tacggcgcgc gtgggctgca ccttccgacg cagcccaaat    720
caggatacgg cccattagta tatattctcc tcctcccagc ccacatcaca tgcgtttcac    780
actttcgcct gtaatcgctc aagtagtcaa gtatgagctc tgtctgcacg aactgattgt    840
cactagtgta ccatcaaact actagacgtg acttgtgata atctcagacg cttatggagt    900
gaaacatggt aaaaaaatgc gtttatgtgc ttgatcactt tgatcagcct tgcctccatc    960
gtctgaaaca tgataagaag acctttgtta cgaaagtgtg aaacgattct gttattcctt   1020
cggctatgcc gtaattcaac tttcacaaag cgatcgcgtt ccatcccaat ggcgcgccga   1080
gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc   1140
cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct   1200
aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc   1260
agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt   1320
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag   1380
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca   1440
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt   1500
tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc   1560
gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct   1620
ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg   1680
tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca   1740
agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact   1800
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta   1860
acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta   1920
actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct   1980
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt   2040
tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga   2100
tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca   2160
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat   2220
caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg   2280
cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt   2340
agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag   2400
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc   2460
gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag   2520
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca   2580
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa   2640
ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga   2700
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata   2760
attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca   2820
agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg   2880
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg   2940
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg   3000
cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag   3060
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac   3120
tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca   3180
tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag   3240
tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta   3300
tcacgaggcc ctttcgtc                                                 3318

<210> 46
<211> 3349
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA769 comprising guide RNA

<400> 46
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgttgt    420
aaaacgacgg ccagtgcgat cgctttgtga aagttgaatt acggcatagc cgaaggaata    480
acagaatcgt ttcacacttt cgtaacaaag gtcttcttat catgtttcag acgatggagg    540
caaggctgat caaagtgatc aagcacataa acgcattttt ttaccatgtt tcactccata    600
agcgtctgag attatcacaa gtcacgtcta gtagtttgat ggtacactag tgacaatcag    660
ttcgtgcaga cagagctcat acttgactac ttgagcgatt acaggcgaaa gtgtgaaacg    720
catgtgatgt gggctgggag gaggagaata tatactaatg ggccgtatcc tgatttgggc    780
tgcgtcggaa ggtgcagccc acgcgcgccg taccgcgcgg gtggcgctgc tacccacttt    840
agtccgttgg atggggatcc gatggtttgc gcggtggcgt tgcgggggat gtttagtacc    900
acatcggaaa ccgaaagacg atggaaccag cttataaacc cgcgcgctgt agtcagcttg    960
aggaagtgca actcttcttg gttttagagc tagaaatagc aagttaaaat aaggctagtc   1020
cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttttcctgc aggggcgtaa   1080
tcatggtcat agcatcccaa tggcgcgccg agcttggcgt aatcatggtc atagctgttt   1140
cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag   1200
tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg   1260
cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg   1320
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc   1380
tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc   1440
acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg   1500
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat   1560
cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag   1620
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga   1680
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg   1740
tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt   1800
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac   1860
gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc   1920
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt   1980
ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc   2040
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc   2100
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg   2160
aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag   2220
atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg   2280
tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt   2340
tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca   2400
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca   2460
gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc   2520
tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt   2580
ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg   2640
gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc   2700
aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg   2760
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga   2820
tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga   2880
ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta   2940
aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg   3000
ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact   3060
ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata   3120
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt   3180
tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa   3240
ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt   3300
atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc               3349

<210> 47
<211> 3645
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA791 comprising donor DNA

<400> 47
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgcgat cgcttctccc aaaattatgc    420
aattttgagg ctcctctaca tcattataat tccccaatac attgctcttt attcttaaca    480
gctttgatcg cgaaatttaa cattttaatt cttgagctgt tattttgtag catcagttta    540
tcatgagcca tgtttggtac taaatataca atcccttggg tttatttgtt tccaagcatg    600
tcattaactt atcttaatgt ggacaagaaa ctgatgcctg cttacattgc tattatttca    660
agcgggtatt gatcctttga catgtgattg atcatttttt tttctctggt tattagggca    720
caacagtggt ggacaacttg ctgaacagtg aggatgttca ctacatgctt gaggccctga    780
aagccctcgg gctctctgtg gaagcagata aagttgcaaa aagagctgta gtcgttggct    840
gtggtggcaa gtttcctgtt gagaaggatg cgaaagagga agtgcaactc ttcttgggaa    900
acgctggaat tgcaatgcga tcgttgacag cagccgtgac tgctgctggt ggaaatgcaa    960
cgtatgtttt tttttaatgt ttatgaaaat aagtatggaa ttcatggggt atgttttatg   1020
acctttttct ttaccatcag ttatgtgctt gatggagtgc cacgaatgag ggagagaccg   1080
attggtgact tggttgtcgg gttgaaacaa cttggtgcgg atgtcgactg tttccttggc   1140
actgaatgcc cacctgttcg tgtcaaggga attggaggac ttcctggtgg caaggttagt   1200
tactcctaaa ctgcatcctt tgtacttctg tatgcacctc aattctttgt caaccttctg   1260
catttataag gaacattcta tgatgaaatt cgaccttaca ctgcacagta acttgaaatg   1320
tttcatgctt aatcaatatg ccatattcct gccaagctca agcgagcaat atttgtttga   1380
atttggtacc atatttttgt atacctgcag gggcgtaatc atggtcatag ctgtttcctg   1440
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta   1500
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg   1560
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga   1620
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg   1680
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag   1740
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc   1800
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca   1860
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt   1920
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc   1980
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc   2040
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc   2100
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact   2160
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg   2220
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta   2280
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca   2340
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa   2400
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg   2460
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc   2520
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg   2580
acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat   2640
ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg   2700
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa   2760
taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca   2820
tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc   2880
gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt   2940
cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa   3000
aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat   3060
cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct   3120
tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga   3180
gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag   3240
tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga   3300
gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca   3360
ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg   3420
cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc   3480
agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag   3540
gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca   3600
tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc                   3645

<210> 48
<211> 9186
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA790 encoding a modified Cas9

<400> 48
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gggcccggcc ggccgcgatc    420
gcgcggccgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat    480
tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc    540
agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt    600
actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa    660
ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt    720
tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca    780
tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt    840
attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat    900
aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag    960
aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa   1020
acgccgtcga tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg   1080
ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc   1140
cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag   1200
acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg   1260
attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc   1320
cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat   1380
ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc   1440
cccccctctc taccttctct agatcggcgt tccggtccat gcttagggcc cggtagttct   1500
acttctgtcc atgtttgtgt tagatccgtg tttgtgttag atccgtgcta ctagcgttcg   1560
tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct   1620
ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt   1680
ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac   1740
ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg   1800
ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta   1860
attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat   1920
ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag   1980
agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt   2040
tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg   2100
tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta   2160
ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc   2220
atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat   2280
aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt   2340
tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca   2400
ccctgttgtt tggtgttact tctgcagcca tggcccctaa gaagaaacga aaggtaggca   2460
tccacggtgt tccagcggct atggacaaga agtacagcat cgggctggac attggcacga   2520
actctgttgg gtgggcggtt atcactgacg agtacaaggt gccgtcgaag aagttcaagg   2580
tgctaggcaa caccgaccgt cacagcatca agaagaacct catcggcgcc ctgttgtttg   2640
actctgggga aaccgccgag gctactcgac taaagagaac cgcccgtagg cgatacacta   2700
ggcggaagaa caggatctgt tatctccagg agatcttcag caacgagatg gcgaaggtcg   2760
atgactcatt cttccaccgc cttgaggagt ctttcctggt cgaggaggac aaaaagcacg   2820
agcgacaccc gatcttcggg aacatcgttg atgaggtagc ctaccacgag aagtacccta   2880
ctatctacca cctgcgcaag aaactagtgg actccaccga caaggcggac ctccgcctta   2940
tctatctcgc cctcgcccac atgatcaagt ttcgcgggca cttcctcatc gaaggcgatc   3000
tcaatccgga caacagcgac gtggacaagc tattcatcca gctagttcag acctacaacc   3060
agctctttga agagaaccct attaacgcga gcggggtgga cgccaaagcc atcctttccg   3120
ccaggctctc taagagccgg agactcgaga accttatcgc tcagttgccc ggcgagaaga   3180
aaaacggcct tttcggcaac ctgattgccc tgtccttggg cttgacgccg aacttcaaga   3240
gcaacttcga cctagctgag gatgctaaac tgcagctgtc caaggacact tatgatgacg   3300
accttgacaa cctcctggcc caaattgggg atcagtacgc ggacctcttc ctcgctgcca   3360
agaacctctc cgacgcgatt ctgttgtcgg acatcctccg cgtgaacacc gagattacta   3420
aggccccact gtccgcgagc atgatcaaac gctacgacga gcaccatcag gatctaactc   3480
tgctcaaggc gctcgtccgt cagcaactcc ctgagaagta caaggagatc ttcttcgacc   3540
agtccaagaa cggctacgcc ggttacattg atggtggcgc gagccaggag gagttctaca   3600
agttcatcaa gcccatctta gagaagatgg acggcaccga agagcttctc gtgaagctca   3660
acagggagga cctcctaagg aagcagcgca cctttgacaa cgggtctatt cctcatcaga   3720
tccatctcgg cgagctccac gcgattttga gacgccaaga ggacttctac ccgttcttga   3780
aggacaaccg ggagaagatc gagaagatcc tcacgttcag gatcccttat tacgtcggcc   3840
ccctagctag gggtaactcg agattcgcgt ggatgaccag aaagtccgag gaaaccatta   3900
ccccgtggaa ctttgaagag gtggtcgaca agggcgcgag cgcgcaatcg ttcattgagc   3960
ggatgaccaa cttcgacaag aacctaccga acgagaaggt cctgccgaaa cactccctgc   4020
tctacgagta cttcaccgtt tataacgagc tgacgaaggt caagtacgtg actgagggca   4080
tgcgtaaacc agcgttcctc agcggtgagc agaagaaggc tatcgtggac ctgctcttta   4140
agacgaaccg caaggtgacc gtgaagcagc tcaaggagga ttatttcaag aagatcgagt   4200
gcttcgactc agtggagatt agcggcgtgg aggaccgttt caatgcgagc ttaggtacct   4260
accacgacct cctgaagatc atcaaggaca aggacttcct cgacaacgag gaaaacgagg   4320
acatcctgga ggacatcgtg ctcaccctga ccctctttga agacagggag atgatcgagg   4380
aaaggttgaa gacctacgcg cacctattcg acgacaaggt catgaaacag ctcaagcgga   4440
ggagatacac tggctggggc agactttcgc ggaagctcat caatgggatc agggacaagc   4500
agtccggcaa gaccatcctg gacttcttga agagcgacgg cttcgctaat cgcaacttca   4560
tgcagctcat tcatgacgac tccctaacat tcaaggagga catccaaaag gcgcaagtct   4620
cgggtcaggg ggattctttg cacgagcaca tcgcaaacct cgccggttct ccggccatta   4680
aaaagggcat ccttcagacg gtcaaggtgg tcgacgagct cgtgaaggtt atgggccggc   4740
ataagcccga gaacatagtg atcgagatgg ctagggagaa ccagaccacg caaaagggcc   4800
agaagaactc acgtgagcgc atgaagagga tcgaggaagg tatcaaggag ctgggctctc   4860
agattctcaa ggagcatccg gtcgagaaca cccagcttca gaacgagaag ctgtacctct   4920
actacctcca gaatggccgc gacatgtacg ttgatcagga gttagatatt aatcggctga   4980
gcgactacga cgttgaccac atcgtgcccc agtcgttcct caaggatgac tccatcgaca   5040
acaaggtcct gaccagatcg gacaagaacc gtggtaagag cgacaatgtg ccctccgagg   5100
aggtggtcaa aaagatgaag aactattggc ggcagttgct gaacgccaag ctcatcaccc   5160
agcgaaagtt cgataattta acgaaggccg aacgaggggg cctttccgaa ctcgataagg   5220
ccggtttcat caagcgtcag ctggttgaga cgcgacagat cacgaagcat gtggcccaga   5280
ttctggacag ccggatgaac acgaagtacg acgagaacga caaacttatt cgggaggtga   5340
aggtgataac cctcaagtcc aagttagtgt cggacttccg caaggacttt cagttctata   5400
aagtgcgcga gatcaacaat taccaccacg cccacgacgc ctacttgaat gccgtggtcg   5460
gcacggcgct catcaaaaag taccccaagc tggagagcga gttcgtctac ggcgactaca   5520
aggtttatga cgtcaggaag atgattgcga agtcagagca agagatcggg aaggccaccg   5580
ccaagtactt cttctactcg aacatcatga acttctttaa gaccgagatc accctcgcca   5640
atggggagat ccggaaacgt ccccttatcg agacgaacgg cgagactggc gagatagtct   5700
gggacaaagg tcgcgatttc gccaccgttc gaaaggtgct ctcaatgcct caggtcaata   5760
tcgtgaagaa gaccgaggtt cagacggggg gcttcagcaa ggagtccatc ctccctaagc   5820
gcaactctga caagctgatc gcccggaaaa aggattggga cccgaaaaag tacggcggct   5880
tcgattctcc caccgtagct tatagcgtgc tcgtggttgc caaggtggag aagggcaaga   5940
gcaagaaact gaagtcggtc aaggagttac tcggcatcac aatcatggag cgcagctcgt   6000
ttgaaaagaa cccgatcgac ttcctggagg ccaagggcta taaggaggtg aagaaggacc   6060
tcatcattaa gctgccgaag tacagcttgt tcgagctcga gaatggcagg aagaggatgc   6120
tagcttcggc cggcgaactt caaaaaggga acgagctcgc gttgccgtcg aagtacgtga   6180
acttcctcta cttagctagt cactatgaaa agctgaaggg ctcgccggag gacaacgagc   6240
agaagcagct cttcgtcgag caacacaagc actacctcga cgagatcatc gagcagataa   6300
gtgagttcag caagcgcgtg atcctcgctg atgccaacct cgataaggtg ctcagcgcct   6360
acaataagca ccgagacaag ccgattcggg agcaggcgga gaacatcatt catttattta   6420
cgctcactaa cctaggcgct ccggctgcct tcaagtactt cgacaccacg atcgacagga   6480
agcgctacac cagcaccaaa gaagtcctcg acgcgacgct cattcaccag agcatcacag   6540
ggctctacga gactcggatc gacttgtcgc aattgggtgg ggataagagg ccggccgcga   6600
ctaaaaaggc cggccaagcc aagaaaaaga aatgattgct agcacgcgtc gaagcagatc   6660
gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga   6720
ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga   6780
cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga   6840
tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt   6900
tactagatcc ctgcaggccc gggttaatta aatttaaatg gcgcgccagc ttggcgtaat   6960
catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac   7020
gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa   7080
ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat   7140
gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc   7200
tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg   7260
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag   7320
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc   7380
gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag   7440
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga   7500
ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc   7560
atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg   7620
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt   7680
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca   7740
gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca   7800
ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag   7860
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca   7920
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg   7980
ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa   8040
aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta   8100
tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag   8160
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga   8220
tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac   8280
cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc   8340
ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta   8400
gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac   8460
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat   8520
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa   8580
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg   8640
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag   8700
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc   8760
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct   8820
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat   8880
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg   8940
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc   9000
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta   9060
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg   9120
tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct   9180
ttcgtc                                                              9186

<210> 49
<211> 1401
<212> PRT
<213> Artificial sequence

<220>
<223> Modified Cas9 from plasmid KVA790

<400> 49
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1               5                   10                  15
Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser
            20                  25                  30
Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys
        35                  40                  45
Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu
    50                  55                  60
Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg
65                  70                  75                  80
Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile
                85                  90                  95
Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp
            100                 105                 110
Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys
        115                 120                 125
Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala
    130                 135                 140
Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val
145                 150                 155                 160
Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala
                165                 170                 175
His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn
            180                 185                 190
Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr
        195                 200                 205
Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp
    210                 215                 220
Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu
225                 230                 235                 240
Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly
                245                 250                 255
Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn
            260                 265                 270
Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr
        275                 280                 285
Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala
    290                 295                 300
Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser
305                 310                 315                 320
Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala
                325                 330                 335
Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu
            340                 345                 350
Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe
        355                 360                 365
Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala
    370                 375                 380
Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met
385                 390                 395                 400
Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu
                405                 410                 415
Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His
            420                 425                 430
Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro
        435                 440                 445
Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg
    450                 455                 460
Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala
465                 470                 475                 480
Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu
                485                 490                 495
Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met
            500                 505                 510
Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His
        515                 520                 525
Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val
    530                 535                 540
Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu
545                 550                 555                 560
Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val
                565                 570                 575
Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe
            580                 585                 590
Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
        595                 600                 605
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu
    610                 615                 620
Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu
625                 630                 635                 640
Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr
                645                 650                 655
Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg
            660                 665                 670
Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg
        675                 680                 685
Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly
    690                 695                 700
Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr
705                 710                 715                 720
Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser
                725                 730                 735
Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys
            740                 745                 750
Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met
        755                 760                 765
Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn
    770                 775                 780
Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg
785                 790                 795                 800
Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His
                805                 810                 815
Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr
            820                 825                 830
Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
        835                 840                 845
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu
    850                 855                 860
Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn
865                 870                 875                 880
Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met
                885                 890                 895
Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg
            900                 905                 910
Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu
        915                 920                 925
Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
    930                 935                 940
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr
945                 950                 955                 960
Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys
                965                 970                 975
Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val
            980                 985                 990
Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala
        995                 1000                1005
Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu
    1010                1015                1020
Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala
1025                1030                1035                1040
Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr
                1045                1050                1055
Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly
            1060                1065                1070
Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
        1075                1080                1085
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu
    1090                1095                1100
Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly
1105                1110                1115                1120
Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu
                1125                1130                1135
Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp
            1140                1145                1150
Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys
        1155                1160                1165
Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
    1170                1175                1180
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu
1185                1190                1195                1200
Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro
                1205                1210                1215
Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala
            1220                1225                1230
Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys
        1235                1240                1245
Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly
    1250                1255                1260
Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys
1265                1270                1275                1280
His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg
                1285                1290                1295
Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn
            1300                1305                1310
Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
        1315                1320                1325
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe
    1330                1335                1340
Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu
1345                1350                1355                1360
Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg
                1365                1370                1375
Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala Thr Lys
            1380                1385                1390
Lys Ala Gly Gln Ala Lys Lys Lys Lys
        1395                1400

<210> 50
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS1

<400> 50
ctaggtgtgg agaacataca tgg                                             23

<210> 51
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS2

<400> 51
gaaggaggat gggctaggtg tgg                                             23

<210> 52
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS3

<400> 52
ataggcccta gaataggcac tgg                                             23

<210> 53
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS4

<400> 53
ctcctcatag gccctagaat agg                                             23

<210> 54
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS5

<400> 54
ctattgccag tgcctattct agg                                             23

<210> 55
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS6

<400> 55
ttcctcgtgc tgggcaagtc tgg                                             23

<210> 56
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS7

<400> 56
gaacaaccag catctgggaa tgg                                             23

<210> 57
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS8

<400> 57
atctgggaat ggtggtgcag tgg                                             23

<210> 58
<211> 23
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS9

<400> 58
ccattgacag cagccgtgac tgc                                             23

<210> 59
<211> 24
<212> DNA
<213> Triticum aestivum

<220>
<223> Target site TS10

<400> 59
gaggaagtgc aactcttctt gggg                                            24

<210> 60
<211> 3645
<212> DNA
<213> Artificial sequence

<220>
<223> Plasmid KVA761 comprising donor DNA

<400> 60
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360
tttcccagtc acgacgttgt aaaacgacgg ccagtgcgat cgcttctccc aaaattatgc    420
aattttgagg ctcctctaca tcattataat tccccaatac attgctcttt attcttaaca    480
gctttgatcg cgaaatttaa cattttaatt cttgagctgt tattttgtag catcagttta    540
tcatgagcca tgtttggtac taaatataca atcccttggg tttatttgtt tccaagcatg    600
tcattaactt atcttaatgt ggacaagaaa ctgatgcctg cttacattgc tattatttca    660
agcgggtatt gatcctttga catgtgattg atcatttttt tttctctggt tattagggca    720
caacagtggt ggacaacttg ctgaacagtg aggatgttca ctacatgctt gaggccctga    780
aagccctcgg gctctctgtg gaagcagata aagttgcaaa aagagctgta gtcgttggct    840
gtggtggcaa gtttcctgtt gagaaggatg cgaaagagga agtgcaactc ttcttgggga    900
acgctggaat tgcaatgcga tcgttgacag cagccgtgac tgctgctggt ggaaatgcaa    960
cgtatgtttt tttttaatgt ttatgaaaat aagtatggaa ttcatggggt atgttttatg   1020
acctttttct ttaccatcag ttatgtgctt gatggagtgc cacgaatgag ggagagaccg   1080
attggtgact tggttgtcgg gttgaaacaa cttggtgcgg atgtcgactg tttccttggc   1140
actgaatgcc cacctgttcg tgtcaaggga attggaggac ttcctggtgg caaggttagt   1200
tactcctaaa ctgcatcctt tgtacttctg tatgcacctc aattctttgt caaccttctg   1260
catttataag gaacattcta tgatgaaatt cgaccttaca ctgcacagta acttgaaatg   1320
tttcatgctt aatcaatatg ccatattcct gccaagctca agcgagcaat atttgtttga   1380
atttggtacc atatttttgt atacctgcag gggcgtaatc atggtcatag ctgtttcctg   1440
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta   1500
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg   1560
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga   1620
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg   1680
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag   1740
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc   1800
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca   1860
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt   1920
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc   1980
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc   2040
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc   2100
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact   2160
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg   2220
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta   2280
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca   2340
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa   2400
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg   2460
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc   2520
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg   2580
acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat   2640
ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg   2700
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa   2760
taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca   2820
tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc   2880
gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt   2940
cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa   3000
aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat   3060
cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct   3120
tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga   3180
gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag   3240
tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga   3300
gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca   3360
ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg   3420
cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc   3480
agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag   3540
gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca   3600
tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc                   3645
