Listagem de Sequncias Biolgicas
<110> Petrleo Brasileiro S.A. - Petrobras 

<120> MICRORGANISMOS GENETICAMENTE MODIFICADOS QUE REALIZAM A PRODUO HETERLOGA DE VERSES MODIFICADAS DA PROTENA SURFACTANTE LV-RANASPUMINA-1 (LV-RSN-1), AS VERSES MODIFICADAS DESSA PROTENA SURFACTANTE, OS GENES SINTTICOS QUE CODIFICAM ESSA PROTENA SURFACTANTE, OS CASSETES DE EXPRESSO CONTENDO ESSES GENES SINTTICOS, E OS VETORES DE EXPRESSO CONTENDO ESSES GENES SINTTICOS

<160> 10

<210> 1
<211> 216  
<212> PRT
<213> Leptodactylus vastus
<223> sequncia predita para uma das isoformas da Lv-ranaspumina-1
<400> 1
Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro Gly Thr 
 1			 5			    10			   15

Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu Val Glu 
		  20				25			    30

Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln Glu 
	   35				  40				45

Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu Arg
    50			   55				  60			

Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu Glu Glu
65			    70			   75				 80

Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln Gly Leu
			85			    90			  95

Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe Asn Ala
		 100				105			    110

Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu Pro Lys
	   115			 120				125

Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys Val
    130			  135				 140	

Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys Ile
145			    150			  155				160

Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu Asp Asp
			165			    170			  175

Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe Glu Gly
		 180			     185			    190	

Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys Pro Asn
	  195				 200			    205

Val Lys Asn Ile Asn Val Ser Ser
    210			  215


<210> 2
<211> 648
<212> DNA
<213> Artificial Sequence
<220> 
<221> CDS
<222> (1)...(648)
<223> sequncia codificadora de uma das isoformas da Lv-ranaspumina-1 aps traduo reversa da sequncia predita de aminocidos
<400> 2
ctg ctg gaa ggc ttt ctg gtg ggc ggc ggc gtg ccg ggc ccg ggc acc 48
Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro Gly Thr 
 1			 5			    10			  15

gcg tgc ctg acc aaa gcg ctg aaa gat agc ggc gat ctg ctg gtg gaa 96
Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu Val Glu 
		  20				25			    30

ctg gcg gtg att att tgc gcg tat cag aac ggc aaa gat ctg cag gaa 144
Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln Glu 
	   35				  40				45

cag gat ttt aaa gaa ctg aaa gaa ctg ctg gaa cgc acc ctg gaa cgc 192
Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu Arg
    50			   55				  60			

gcg ggc tgc gcg ctg gat gat att gtg gcg gat ctg ggc ctg gaa gaa 240
Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu Glu Glu
65			    70			   75				 80

ctg ctg ggc agc att ggc gtg agc acc ggc gat att att cag ggc ctg 288
Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln Gly Leu
			85			    90			  95

tat aaa ctg ctg aaa gaa ctg aaa att gat gaa acc gtg ttt aac gcg 336
Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe Asn Ala
		 100			     105			   110

gtg tgc gat gtg acc aaa aaa atg ctg gat aac aaa tgc ctg ccg aaa 384
Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu Pro Lys
	   115			 120			    125

att ctg cag ggc gat ctg gtg aaa ttt ctg gat ctg aaa tat aaa gtg 432
Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys Val
    130			  135				 140	

tgc att gaa ggc ggc gat ccg gaa ctg att att aaa gat ctg aaa att 480
Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys Ile
145			    150			  155				160

att ctg gaa cgc ctg ccg tgc gtg ctg ggc ggc gtg ggc ctg gat gat 528
Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu Asp Asp
			165			   170			 175

ctg ttt aaa aac att ttt gtg aaa gat ggc att ctg agc ttt gaa ggc 576
Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe Glu Gly
		 180			     185			   190	

att gcg aaa ccg ctg ggc gat ctg ctg att ctg gtg ctg tgc ccg aac 624
Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys Pro Asn
	  195				 200			    205

gtg aaa aac att aac gtg agc agc     					    648
Val Lys Asn Ile Asn Val Ser Ser
    210			  215

<210> 3
<211> 651
<212> DNA
<213> Artificial Sequence
<220>
<221> CDS
<222> (1)...(651)
<223> otimizao da frequncia de cdons da SEQ ID NO:2 para expresso em bactrias e adio do cdon de iniciao ATG
<400> 3
atg ctg ctg gaa ggt ttt ctg gtt ggg ggc ggt gtt ccg ggt cca ggc 48
Met Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro Gly
1			 5			    10			  15

acg gcc tgc ttg acg aag gct ctg aaa gat agc ggt gac ctg ctg gtg 96
Thr Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu Val
		  20				25			   30

gag tta gcg gtt att att tgt gca tac cag aat ggc aaa gac ctt cag 144
Glu Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln
	   35				  40				45

gag cag gac ttc aaa gaa ctg aag gaa ttg ctg gaa cgt aca ttg gaa 192
Glu Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu
    50			   55				 60			

cgt gcc ggt tgt gcc ctc gat gat att gtg gcc gat tta ggt ctg gaa 240
Arg Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu Glu
65			    70			   75				 80

gaa ctg ctg ggc tcc atc ggc gtt agt acc ggc gat att atc cag ggt 288
Glu Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln Gly
			85			    90			  95

ctg tat aaa ctg ttg aag gag tta aaa atc gac gag acc gtc ttt aat 336
Leu Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe Asn
		 100			     105			   110

gcg gtc tgc gat gtg acc aaa aaa atg ctg gat aac aag tgc tta ccg 384
Ala Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu Pro
	   115			 120			    125



aaa att ctg caa gga gat ctg gta aag ttc ctt gat ctg aag tat aaa 432
Lys Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys
    130			  135				 140	

gtt tgt att gaa ggt ggc gat cca gaa ctg att att aag gat ctg aaa 480
Val Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys
145			    150			  155				160

atc atc ctg gaa cgg ctt ccg tgt gtg ttg ggt gga gtc ggt ttg gat 528
Ile Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu Asp
			165			   170			 175

gat ctc ttt aag aac att ttt gtt aag gat ggg att ctg tcc ttc gaa 576
Asp Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe Glu
		 180			     185			   190	

ggt att gcg aaa cct ctt ggt gac ctt ctc atc ctt gtc tta tgc ccg 624
Gly Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys Pro
	  195				 200			    205

aac gtc aag aat atc aat gta tcc tct					    651
Asn Val Lys Asn Ile Asn Val Ser Ser
    210			  215

<210> 4
<211> 697 
<212> DNA
<213> Artificial Sequence
<220> CDS
<223> a SEQ ID NO:3 incluindo o stio de restrio para NdeI, da sequncia codificadora da etiqueta de poli-histidina, do stio de restrio para EcoRI, da sequncia que codifica o stio de clivagem para TEV, do stio de restrio para NdeI, e do stio de restrio para XhoI
<400> 4
g   aat tct gaa aac ttg tat ttc cag ggc agc cat atg atg ctg ctg 46
    Asn Ser Glu Asn Leu Tyr Phe Gln Gly Ser His Met Met Leu Leu
     1		     5			  10				15

gaa ggt ttt ctg gtt ggg ggc ggt gtt ccg ggt cca ggc acg gcc tgc 94
Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro Gly Thr Ala Cys
			20			    25			  30

ttg acg aag gct ctg aaa gat agc ggt gac ctg ctg gtg gag tta gcg 142
Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu Val Glu Leu Ala
		 35				40			   45

gtt att att tgt gca tac cag aat ggc aaa gac ctt cag gag cag gac 190
Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln Glu Gln Asp
	   50				 55			     60

ttc aaa gaa ctg aag gaa ttg ctg gaa cgt aca ttg gaa cgt gcc ggt 238
Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu Arg Ala Gly
    65			   70				 75			

tgt gcc ctc gat gat att gtg gcc gat tta ggt ctg gaa gaa ctg ctg 286
Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu Glu Glu Leu Leu
80			    85			  90				95

ggc tcc atc ggc gtt agt acc ggc gat att atc cag ggt ctg tat aaa 334
Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln Gly Leu Tyr Lys
		     100			   105			 110

ctg ttg aag gag tta aaa atc gac gag acc gtc ttt aat gcg gtc tgc 382
Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe Asn Ala Val Cys
		 115			     120 			   125

gat gtg acc aaa aaa atg ctg gat aac aag tgc tta ccg aaa att ctg 430
Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu Pro Lys Ile Leu
	   130			 135			     140	

caa gga gat ctg gta aag ttc ctt gat ctg aag tat aaa gtt tgt att 478
Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys Val Cys Ile
    145			  150				155

gaa ggt ggc gat cca gaa ctg att att aag gat ctg aaa atc atc ctg 526
Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys Ile Ile Leu
160			    165			  170				175

gaa cgg ctt ccg tgt gtg ttg ggt gga gtc ggt ttg gat gat ctc ttt 574
Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu Asp Asp Leu Phe
			180			   185			  190

aag aac att ttt gtt aag gat ggg att ctg tcc ttc gaa ggt att gcg 622
Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe Glu Gly Ile Ala
		 195			     200			   205

aaa cct ctt ggt gac ctt ctc atc ctt gtc tta tgc ccg aac gtc aag 670
Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys Pro Asn Val Lys
	  210				 215			    220
aat atc aat gta tcc tct taactcgag					    697
Asn Ile Asn Val Ser Ser
    225

<210> 5
<211> 6395
<212> DNA
<213> Artificial Sequence
<220> 
<223> vetor de expresso pPBUFCBac-LvRsn1 resultante da insero da SEQ ID NO:4 na SEQ ID NO:5
<400> 5
gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat 60
tcccctctag aaataatttt gtttaaactt taagaaggag atatacatat g  cat cat 117
    His His
									     1

cat cat cat cac gtg aat tct gaa aac ttg tat ttc cag ggc agc cat 165
His His His His Val Asn Ser Glu Asn Leu Tyr Phe Gln Gly Ser His
	  5			    10			 15

atg atg ctg ctg gaa ggt ttt ctg gtt ggg ggc ggt gtt ccg ggt cca 213
Met Met Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro
    20 			 25			  30	

ggc acg gcc tgc ttg acg aag gct ctg aaa gat agc ggt gac ctg ctg 261
Gly Thr Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu
 35			  40			    45			 50

gtg gag tta gcg gtt att att tgt gca tac cag aat ggc aaa gac ctt 309
Val Glu Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu
		    55			 60			  65

cag gag cag gac ttc aaa gaa ctg aag gaa ttg ctg gaa cgt aca ttg 357
Gln Glu Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu
		70			  75			    80

gaa cgt gcc ggt tgt gcc ctc gat gat att gtg gcc gat tta ggt ctg 405
Glu Arg Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu
	  85			    90			95

gaa gaa ctg ctg ggc tcc atc ggc gtt agt acc ggc gat att atc cag 453
Glu Glu Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln
    100        		105			  110

ggt ctg tat aaa ctg ttg aag gag tta aaa atc gac gag acc gtc ttt 501
Gly Leu Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe
115			  120			    125			130

aat gcg gtc tgc gat gtg acc aaa aaa atg ctg gat aac aag tgc tta 549
Asn Ala Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu
		    135			140			  145



ccg aaa att ctg caa gga gat ctg gta aag ttc ctt gat ctg aag tat 597
Pro Lys Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr
		150			  155			    160

aaa gtt tgt att gaa ggt ggc gat cca gaa ctg att att aag gat ctg 645
Lys Val Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu
	  165			    170			175

aaa atc atc ctg gaa cgg ctt ccg tgt gtg ttg ggt gga gtc ggt ttg 693
Lys Ile Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu
    180			185			  190

gat gat ctc ttt aag aac att ttt gtt aag gat ggg att ctg tcc ttc 741
Asp Asp Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe
195			  200			    205			210

gaa ggt att gcg aaa cct ctt ggt gac ctt ctc atc ctt gtc tta tgc 789
Glu Gly Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys
		    215			220			  225

ccg aac gtc aag aat atc aat gta tcc tct    taactcgaga tcgatgatat 819
Pro Asn Val Lys Asn Ile Asn Val Ser Ser
	      230			  235

tcgagcctag gtataatcgg atccggctgc taacaaagcc cgaaaggaag ctgagttggc 879
tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag 939
gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc 999
ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc 1059
gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta 1119
ccgcattaaa gctagcttat cgatgataag ctgtcaaaca tgagaattaa ttcttgaaga 1179
cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 1239
tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 1299
taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 1359
tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 1419
gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 1479
gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 1539
cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 1599
tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac 1659
tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 1719
atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 1779
ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 1839
gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 1899
gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc 1959
gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 2019
gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 2079
gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 2139
cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 2199
atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 2259
tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 2319
ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 2379
gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 2439
tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2499
ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 2559
ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2619
gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2679
ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2739
tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2799
ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2859
agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2919
agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 2979
gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3039
tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 3099
accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 3159
gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 3219
atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 3279
agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac 3339
acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 3399
gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 3459
gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc 3519
atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg 3579
ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt 3639
tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac 3699
tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat 3759
gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt 3819
aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca 3879
gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt 3939
tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg 3999
tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag 4059
gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg 4119
gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac 4179
agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg 4239
ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg 4299
aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc 4359
gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga 4419
gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg 4479
gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc 4539
atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg 4599
gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga 4659
gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag 4719
cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc 4779
atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 4839
gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg 4899
agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4959
tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5019
cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg 5079
gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg 5139
tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac 5199
taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag 5259
cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg 5319
catggtttgt tgaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 5379
atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 5439
cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 5499
cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 5559
acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 5619
tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 5679
gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 5739
agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 5799
ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 5859
ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 5919
acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 5979
gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 6039
cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 6099
ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag 6159
caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac 6219
ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg 6279
atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 6339
gatgccggcc acgatgcgtc cggcgtagag gatcga 					6375

<210> 6
<211> 236
<212> PRT
<213> Artificial Sequence
<220> 
<223> sequncia de aminocidos da verso modificada da protena surfactante Lv-Rsn-1 codificada pela sequncia de nucleotdeos SEQ ID NO:4, que compreende a SEQ ID NO:3
<400> 6
His His His His His His Val Asn Ser Glu Asn Leu Tyr Phe Gln Gly 
1			 5			    10			  15

Ser His Met Met Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro 
		  20				25			   30

Gly Pro Gly Thr Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp 
	   35				 40			     45

Leu Leu Val Glu Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys 
    50			   55				 60

Asp Leu Gln Glu Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg 
65			    70			   75				 80

Thr Leu Glu Arg Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu 
			85			    90			  95

Gly Leu Glu Glu Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile 
		 100			     105			   110

Ile Gln Gly Leu Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr 
	   115			 120			    125

Val Phe Asn Ala Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys 
    130			  135				140	

Cys Leu Pro Lys Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu 
145			    150			  155				160

Lys Tyr Lys Val Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys 
			165			   170			 175

Asp Leu Lys Ile Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val 
		 180			     185			   190

Gly Leu Asp Asp Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu 
	  195				 200			    205

Ser Phe Glu Gly Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val 
    210			  215				 220

Leu Cys Pro Asn Val Lys Asn Ile Asn Val Ser Ser
225			    230			  235

<210> 7
<211> 648
<212> DNA
<213> Artificial Sequence
<220>
<221> CDS
<222> (1)...(648)
<223> otimizao da frequncia de cdons da SEQ ID NO:2 para expresso em leveduras
<400> 7
ttg ttg gaa gga ttt ttg gtc gga ggt ggt gtc cct ggt cct ggt aca 48
Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly Pro Gly Thr
1			 5			    10			  15

gca tgt ttg act aag gca ttg aaa gac agt gga gac ttg ttg gtt gag 96
Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu Leu Val Glu
		  20				25			   30

ttg gct gtt att att tgt gct tac caa aac ggt aaa gat ttg caa gag 144
Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln Glu
	   35				  40			     45

caa gat ttc aag gaa ttg aag gag ttg ttg gaa aga act ttg gaa aga 192
Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu Arg
    50			   55				 60			

gct ggt tgt gct ttg gat gat att gtt gct gat ttg ggt ttg gaa gag 240
Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly Leu Glu Glu
65			    70			   75				 80

ttg ttg ggt tct att ggt gtt tct act gga gat atc atc caa ggt ttg 288
Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile Gln Gly Leu
			85			    90			  95

tac aag ttg ttg aag gag ttg aag atc gat gaa act gtt ttt aac gct 336
Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val Phe Asn Ala
		 100				105			   110

gtt tgt gat gtt act aag aaa atg ttg gat aac aag tgt ttg cca aag 384
Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys Leu Pro Lys
	   115			 120			    125

atc ttg caa gga gat ttg gtt aag ttc ttg gat ttg aag tac aag gtt 432
Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys Val
    130			  135				 140

tgt atc gaa ggt gga gat cca gaa ttg att att aag gat ttg aag atc 480
Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys Ile
145			    150			  155				160


atc ttg gag aga ttg cct tgt gtt ttg ggt ggt gtt ggt ttg gat gat 528
Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly Leu Asp Asp
			165			    170			 175

ttg ttt aaa aac atc ttc gtt aag gat ggt att ttg tct ttc gaa ggt 576
Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser Phe Glu Gly
		 180			     185			   190

att gct aag cct ttg gga gat ttg ttg att ttg gtt ttg tgt cct aat 624
Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu Cys Pro Asn
	  195				 200			    205

gtc aag aat atc aat gtt tca tca						    648
Val Lys Asn Ile Asn Val Ser Ser
    210			  215

<210> 8
<211> 685
<212> DNA
<213> Artificial Sequence
<220>
<221> CDS
<222> (1)...(648)
<223> a SEQ ID NO:8 aps a adio do stio de restrio para a endonuclease PstI, de dois nucleotdeos para colocar a sequncia codificadora no mesmo quadro de traduo do fator de secreo alfa, e do stio de restrio para a endonuclease NotI
<400> 8
ct  gca gga ttg ttg gaa gga ttt ttg gtc gga ggt ggt gtc cct ggt 47
    Ala Gly Leu Leu Glu Gly Phe Leu Val Gly Gly Gly Val Pro Gly
     1		     5			  10				15

cct ggt aca gca tgt ttg act aag gca ttg aaa gac agt gga gac ttg 95
Pro Gly Thr Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser Gly Asp Leu
			20			    25			  30

ttg gtt gag ttg gct gtt att att tgt gct tac caa aac ggt aaa gat 143
Leu Val Glu Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn Gly Lys Asp
		 35				40			   45

ttg caa gag caa gat ttc aag gaa ttg aag gag ttg ttg gaa aga act 191
Leu Gln Glu Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu Glu Arg Thr
	   50				 55			     60

ttg gaa aga gct ggt tgt gct ttg gat gat att gtt gct gat ttg ggt 239
Leu Glu Arg Ala Gly Cys Ala Leu Asp Asp Ile Val Ala Asp Leu Gly
    65			   70				 75	

ttg gaa gag ttg ttg ggt tct att ggt gtt tct act gga gat atc atc 287
Leu Glu Glu Leu Leu Gly Ser Ile Gly Val Ser Thr Gly Asp Ile Ile
80			    85			  90				95

caa ggt ttg tac aag ttg ttg aag gag ttg aag atc gat gaa act gtt 335
Gln Gly Leu Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp Glu Thr Val
		     100			   105			 110

ttt aac gct gtt tgt gat gtt act aag aaa atg ttg gat aac aag tgt 383
Phe Asn Ala Val Cys Asp Val Thr Lys Lys Met Leu Asp Asn Lys Cys
		 115			     120 			   125

ttg cca aag atc ttg caa gga gat ttg gtt aag ttc ttg gat ttg aag 431
Leu Pro Lys Ile Leu Gln Gly Asp Leu Val Lys Phe Leu Asp Leu Lys
	   130			 135			     140


tac aag gtt tgt atc gaa ggt gga gat cca gaa ttg att att aag gat 479
Tyr Lys Val Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile Ile Lys Asp
    145			  150				155

ttg aag atc atc ttg gag aga ttg cct tgt gtt ttg ggt ggt gtt ggt 527
Leu Lys Ile Ile Leu Glu Arg Leu Pro Cys Val Leu Gly Gly Val Gly
160			    165			  170				175

ttg gat gat ttg ttt aaa aac atc ttc gtt aag gat ggt att ttg tct 575
Leu Asp Asp Leu Phe Lys Asn Ile Phe Val Lys Asp Gly Ile Leu Ser
			180			   185			  190

ttc gaa ggt att gct aag cct ttg gga gat ttg ttg att ttg gtt ttg 623
Phe Glu Gly Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile Leu Val Leu
		 195			     200			   205

tgt cct aat gtc aag aat atc aat gtt tca tca gag aac ctt tac ttt 671
Cys Pro Asn Val Lys Asn Ile Asn Val Ser Ser Glu Asn Leu Tyr Phe
	  210				 215			    220

cag gga gcg gcc gc								     685
Gln Gly Ala Ala
    225

<210> 9
<211> 4219
<212> DNA
<213> Artificial Sequence
<220> 
<223> vetor de expresso pPBUFCYea-LvRsn1 resultante da insero da SEQ ID NO:9 na SEQ ID NO:10
<400> 9
agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 60
gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 120
tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 180
agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 240
acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 300
tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 360
agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 420
gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 480
ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt 540
cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 600
ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 660
ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 720
gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 780
atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 840
actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 900
caacttgaga agatcaaaaa acaactaatt attcgaaacg   atg aga ttt cct tca 955
    Met Arg Phe Pro Ser
     1		   5

att ttt act gct gtt tta ttc gca gca tcc tcc gca tta gct gct cca 1003
Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser Ala Leu Ala Ala Pro
		    10			 15 			  20

gtc aac act aca aca gaa gat gaa acg gca caa att ccg gct gaa gct 1051
Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala Glu Ala
		25			  30			    35

gtc atc ggt tac tca gat tta gaa ggg gat ttc gat gtt gct gtt ttg 1099
Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe Asp Val Ala Val Leu
	  40			    45			50

cca ttt tcc aac agc aca aat aac ggg tta ttg ttt ata aat act act 1147
Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn Thr Thr
    55			60			  65

att gcc agc att gct gct aaa gaa gaa ggg gta tct ctc gag aaa aga 1195
Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val Ser Leu Glu Lys Arg
70			  75			    80			85

gag gct gaa gct gca gga ttg ttg gaa gga ttt ttg gtc gga ggt ggt 1243
Glu Ala Glu Ala Ala Gly Leu Leu Glu Gly Phe Leu Val Gly Gly Gly
		    90			95			  100

gtc cct ggt cct ggt aca gca tgt ttg act aag gca ttg aaa gac agt 1291
Val Pro Gly Pro Gly Thr Ala Cys Leu Thr Lys Ala Leu Lys Asp Ser
		105			  110			    115

gga gac ttg ttg gtt gag ttg gct gtt att att tgt gct tac caa aac 1339
Gly Asp Leu Leu Val Glu Leu Ala Val Ile Ile Cys Ala Tyr Gln Asn
	  120			    125			130

ggt aaa gat ttg caa gag caa gat ttc aag gaa ttg aag gag ttg ttg 1387
Gly Lys Asp Leu Gln Glu Gln Asp Phe Lys Glu Leu Lys Glu Leu Leu
    135			140			  145

gaa aga act ttg gaa aga gct ggt tgt gct ttg gat gat att gtt gct 1435
Glu Arg Thr Leu Glu Arg Ala Gly Cys Ala Leu Asp Asp Ile Val Ala
150			  155			    160			165

gat ttg ggt ttg gaa gag ttg ttg ggt tct att ggt gtt tct act gga 1483
Asp Leu Gly Leu Glu Glu Leu Leu Gly Ser Ile Gly Val Ser Thr Gly
		    170			175			  180

gat atc atc caa ggt ttg tac aag ttg ttg aag gag ttg aag atc gat 1531
Asp Ile Ile Gln Gly Leu Tyr Lys Leu Leu Lys Glu Leu Lys Ile Asp
		185			  190			    195

gaa act gtt ttt aac gct gtt tgt gat gtt act aag aaa atg ttg gat 1579
Glu Thr Val Phe Asn Ala Val Cys Asp Val Thr Lys Lys Met Leu Asp
	  200			    205			210

aac aag tgt ttg cca aag atc ttg caa gga gat ttg gtt aag ttc ttg 1627
Asn Lys Cys Leu Pro Lys Ile Leu Gln Gly Asp Leu Val Lys Phe Leu
    215			220			  225

gat ttg aag tac aag gtt tgt atc gaa ggt gga gat cca gaa ttg att 1675
Asp Leu Lys Tyr Lys Val Cys Ile Glu Gly Gly Asp Pro Glu Leu Ile
230			  235			    240			245

att aag gat ttg aag atc atc ttg gag aga ttg cct tgt gtt ttg ggt 1723
Ile Lys Asp Leu Lys Ile Ile Leu Glu Arg Leu Pro Cys Val Leu Gly
		    250			255			  260

ggt gtt ggt ttg gat gat ttg ttt aaa aac atc ttc gtt aag gat ggt 1771
Gly Val Gly Leu Asp Asp Leu Phe Lys Asn Ile Phe Val Lys Asp Gly
		265			  270			    275

att ttg tct ttc gaa ggt att gct aag cct ttg gga gat ttg ttg att 1819
Ile Leu Ser Phe Glu Gly Ile Ala Lys Pro Leu Gly Asp Leu Leu Ile
	  280			    285			290

ttg gtt ttg tgt cct aat gtc aag aat atc aat gtt tca tca gag aac 1867
Leu Val Leu Cys Pro Asn Val Lys Asn Ile Asn Val Ser Ser Glu Asn
    295			300			  305


ctt tac ttt cag gga gcg gcc gcc agc ttt cta gaa caa aaa ctc atc 1915
Leu Tyr Phe Gln Gly Ala Ala Ala Ser Phe Leu Glu Gln Lys Leu Ile
310			  315	 		    320			325

tca gaa gag gat ctg aat agc gcc gtc gac cat cat cat cat cat cat 1963
Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His His His His
		    330			335			  340

tgagtttgta gccttagaca tgactgttcc tcagttcaag ttgggcactt acgagaagac 2023
cggtcttgct agattctaat caagaggatg tcagaatgcc atttgcctga gagatgcagg 2083
cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt 2143
tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagctgat gaatatcttg 2203
tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc 2263
ttcagagtac agaagattaa gtgagacctt cgtttgtgcg gatcccccac acaccatagc 2323
ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc 2383
gtaccacttc aaaacaccca agcacagcat actaaatttt ccctctttct tcctctaggg 2443
tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt 2503
ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaattttttt 2563
ttttagtttt tttctctttc agtgacctcc attgatattt aagttaataa acggtcttca 2623
atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta cttcttgttc 2683
attagaaaga aagcatagca atctaatcta aggggcggtg ttgacaatta atcatcggca 2743
tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 2803
agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 2863
cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 2923
gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 2983
gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 3043
ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 3103
ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 3163
cacgtccgac ggcggcccac gggtcccagg cctcggagat ccgtccccct tttcctttgt 3223
cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct 3283
aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt 3343
tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg 3403
cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa 3463
ggctttaatt tgcaagctgg agaccaacat gtgagcaaaa ggccagcaaa aggccaggaa 3523
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3583
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3643
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3703
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 3763
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3823
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3883
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 3943
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4003
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 4063
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4123
aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4183
cgaaaactca cgttaaggga ttttggtcat gagatc                 		4219

<210> 10
<211> 341
<212> PRT
<213> Artificial Sequence
<220> 
<223> sequncia de aminocidos da verso modificada da protena surfactante Lv-Rsn-1 codificada pela sequncia de nucleotdeos SEQ ID NO:9, que por sua vez est contida na SEQ ID NO:11.
<400> 10
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1			 5			    10			  15

Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
		  20				25			   30

Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
	   35				  40			     45

Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50			   55				 60

Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65			    70			   75				 80

Ser Leu Glu Lys Arg Glu Ala Glu Ala Ala Gly Leu Leu Glu Gly Phe 
			85			    90			  95

Leu Val Gly Gly Gly Val Pro Gly Pro Gly Thr Ala Cys Leu Thr Lys 
		 100				105			   110

Ala Leu Lys Asp Ser Gly Asp Leu Leu Val Glu Leu Ala Val Ile Ile 
	   115			 120			    125

Cys Ala Tyr Gln Asn Gly Lys Asp Leu Gln Glu Gln Asp Phe Lys Glu 
    130			  135				 140

Leu Lys Glu Leu Leu Glu Arg Thr Leu Glu Arg Ala Gly Cys Ala Leu
145			    150			  155				160

Asp Asp Ile Val Ala Asp Leu Gly Leu Glu Glu Leu Leu Gly Ser Ile 
			165			    170			 175

Gly Val Ser Thr Gly Asp Ile Ile Gln Gly Leu Tyr Lys Leu Leu Lys 
		 180			     185			   190

Glu Leu Lys Ile Asp Glu Thr Val Phe Asn Ala Val Cys Asp Val Thr 
	  195				 200			    205

Lys Lys Met Leu Asp Asn Lys Cys Leu Pro Lys Ile Leu Gln Gly Asp 
    210			  215				220

Leu Val Lys Phe Leu Asp Leu Lys Tyr Lys Val Cys Ile Glu Gly Gly
225			    230			  235				240

Asp Pro Glu Leu Ile Ile Lys Asp Leu Lys Ile Ile Leu Glu Arg Leu 
			245			   250			 255

Pro Cys Val Leu Gly Gly Val Gly Leu Asp Asp Leu Phe Lys Asn Ile 
		 260			     265			   270

Phe Val Lys Asp Gly Ile Leu Ser Phe Glu Gly Ile Ala Lys Pro Leu 
	   275			 280			    285

Gly Asp Leu Leu Ile Leu Val Leu Cys Pro Asn Val Lys Asn Ile Asn 
    290			  295				300

Val Ser Ser Glu Asn Leu Tyr Phe Gln Gly Ala Ala Ala Ser Phe Leu
305			    310			  315				320

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His 
		     325			    330			  335

His His His His His
		 340

