                         SEQUENCE LISTING

<110>  Northwestern University
       Ozdinler, P. Hande
       Jara, Javier H.
       Genc, Baris
 
<120>  MODULATION OF UBIQUITIN CARBOXY-TERMINAL HYDROLASE LIGASE 1 
       (UCHL1) EXPRESSION FOR TREATING NEUROLOGICAL DISEASE, DISORDERS, 
       AND INJURIES ASSOCIATED WITH UPPER MOTOR NEURONS

<130>  702581.01881

<150>  62/947,189
<151>  2019-12-12

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  223
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Leu Lys Pro Met Glu Ile Asn Pro Glu Met Leu Asn Lys Val 
1               5                   10                  15      


Leu Ser Arg Leu Gly Val Ala Gly Gln Trp Arg Phe Val Asp Val Leu 
            20                  25                  30          


Gly Leu Glu Glu Glu Ser Leu Gly Ser Val Pro Ala Pro Ala Cys Ala 
        35                  40                  45              


Leu Leu Leu Leu Phe Pro Leu Thr Ala Gln His Glu Asn Phe Arg Lys 
    50                  55                  60                  


Lys Gln Ile Glu Glu Leu Lys Gly Gln Glu Val Ser Pro Lys Val Tyr 
65                  70                  75                  80  


Phe Met Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr Ile Gly Leu Ile 
                85                  90                  95      


His Ala Val Ala Asn Asn Gln Asp Lys Leu Gly Phe Glu Asp Gly Ser 
            100                 105                 110         


Val Leu Lys Gln Phe Leu Ser Glu Thr Glu Lys Met Ser Pro Glu Asp 
        115                 120                 125             


Arg Ala Lys Cys Phe Glu Lys Asn Glu Ala Ile Gln Ala Ala His Asp 
    130                 135                 140                 


Ala Val Ala Gln Glu Gly Gln Cys Arg Val Asp Asp Lys Val Asn Phe 
145                 150                 155                 160 


His Phe Ile Leu Phe Asn Asn Val Asp Gly His Leu Tyr Glu Leu Asp 
                165                 170                 175     


Gly Arg Met Pro Phe Pro Val Asn His Gly Ala Ser Ser Glu Asp Thr 
            180                 185                 190         


Leu Leu Lys Asp Ala Ala Lys Val Cys Arg Glu Phe Thr Glu Arg Glu 
        195                 200                 205             


Gln Gly Glu Val Arg Phe Ser Ala Val Ala Leu Cys Lys Ala Ala 
    210                 215                 220             


<210>  2
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr Ile Gly Leu Ile His 
1               5                   10                  15      


Ala Val Ala Asn Asn Gln Asp Lys Leu Gly Phe Glu Asp Gly Ser Val 
            20                  25                  30          


Leu Lys Gln Phe Leu Ser Glu Thr Glu Lys Met Ser Pro Glu Asp Arg 
        35                  40                  45              


Ala Lys Cys Phe Glu Lys Asn Glu Ala Ile Gln Ala Ala His Asp Ala 
    50                  55                  60                  


Val Ala Gln Glu Gly Gln Cys Arg Val Asp Asp Lys Val Asn Phe His 
65                  70                  75                  80  


Phe Ile Leu Phe Asn Asn Val Asp Gly His Leu Tyr Glu Leu Asp Gly 
                85                  90                  95      


Arg Met Pro Phe Pro Val Asn His Gly Ala Ser Ser Glu Asp Thr Leu 
            100                 105                 110         


Leu Lys Asp Ala Ala Lys Val Cys Arg Glu Phe Thr Glu Arg Glu Gln 
        115                 120                 125             


Gly Glu Val Arg Phe Ser Ala Val Ala Leu Cys Lys Ala Ala 
    130                 135                 140         


<210>  3
<211>  126
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr Ile Gly Leu Ile His 
1               5                   10                  15      


Ala Val Ala Asn Asn Gln Asp Lys Leu Gly Phe Glu Asp Gly Ser Val 
            20                  25                  30          


Leu Lys Gln Phe Leu Ser Glu Thr Glu Lys Met Ser Pro Glu Asp Arg 
        35                  40                  45              


Ala Lys Cys Phe Glu Lys Asn Glu Val Asp Asp Lys Val Asn Phe His 
    50                  55                  60                  


Phe Ile Leu Phe Asn Asn Val Asp Gly His Leu Tyr Glu Leu Asp Gly 
65                  70                  75                  80  


Arg Met Pro Phe Pro Val Asn His Gly Ala Ser Ser Glu Asp Thr Leu 
                85                  90                  95      


Leu Lys Asp Ala Ala Lys Val Cys Arg Glu Phe Thr Glu Arg Glu Gln 
            100                 105                 110         


Gly Glu Val Arg Phe Ser Ala Val Ala Leu Cys Lys Ala Ala 
        115                 120                 125     


<210>  4
<211>  221
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Gln Leu Lys Pro Met Glu Ile Asn Pro Glu Val Ser Val Leu Ser 
1               5                   10                  15      


Arg Leu Gly Val Ala Gly Gln Trp Arg Phe Val Asp Val Leu Gly Leu 
            20                  25                  30          


Glu Glu Glu Ser Leu Gly Ser Val Pro Ala Pro Ala Cys Ala Leu Leu 
        35                  40                  45              


Leu Leu Phe Pro Leu Thr Ala Gln His Glu Asn Phe Arg Lys Lys Gln 
    50                  55                  60                  


Ile Glu Glu Leu Lys Gly Gln Glu Val Ser Pro Lys Val Tyr Phe Met 
65                  70                  75                  80  


Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr Ile Gly Leu Ile His Ala 
                85                  90                  95      


Val Ala Asn Asn Gln Asp Lys Leu Gly Phe Glu Asp Gly Ser Val Leu 
            100                 105                 110         


Lys Gln Phe Leu Ser Glu Thr Glu Lys Met Ser Pro Glu Asp Arg Ala 
        115                 120                 125             


Lys Cys Phe Glu Lys Asn Glu Ala Ile Gln Ala Ala His Asp Ala Val 
    130                 135                 140                 


Ala Gln Glu Gly Gln Cys Arg Val Asp Asp Lys Val Asn Phe His Phe 
145                 150                 155                 160 


Ile Leu Phe Asn Asn Val Asp Gly His Leu Tyr Glu Leu Asp Gly Arg 
                165                 170                 175     


Met Pro Phe Pro Val Asn His Gly Ala Ser Ser Glu Asp Thr Leu Leu 
            180                 185                 190         


Lys Asp Ala Ala Lys Val Cys Arg Glu Phe Thr Glu Arg Glu Gln Gly 
        195                 200                 205             


Glu Val Arg Phe Ser Ala Val Ala Leu Cys Lys Ala Ala 
    210                 215                 220     


<210>  5
<211>  403
<212>  PRT
<213>  Homo sapiens

<400>  5

Gly Ser Ala Ser Cys Phe Ser Ser Ser Leu Gly Tyr Phe Cys Arg Ala 
1               5                   10                  15      


Leu Arg Glu Asp Ala Ala Gln Ala Asp Gly Asp Gln Pro Arg Asp Ala 
            20                  25                  30          


Glu Gln Ser Glu Trp Arg Leu Ala Pro Ser Leu Ala Pro Ser Pro Ala 
        35                  40                  45              


Ser Ala Glu Ala Gly Ala Pro Thr Gly Ser Gly Cys Trp Gln Gly Pro 
    50                  55                  60                  


Ser Arg Pro Leu Arg Ala Pro Glu Thr Ala Gly Leu Gly Arg Gly Leu 
65                  70                  75                  80  


Gly Ala Phe Pro Gly Pro Leu His Leu Ala Gly Asp Ser Thr Lys Pro 
                85                  90                  95      


Val Thr Gly Arg Arg Arg Gly Leu Arg Pro Val Ala Ser Glu Arg Gln 
            100                 105                 110         


Ala Glu Leu Pro Arg Ala Trp Arg Gly Gln His Arg Leu Gly Cys Thr 
        115                 120                 125             


Gly Phe Ala Gly Ala Thr Cys Gly Pro Arg Phe Val Leu Cys His Cys 
    130                 135                 140                 


Ala Gly Pro Gly Gly Gly Gly Arg Ala Gly Leu Gly Leu Leu Pro Gly 
145                 150                 155                 160 


Ser Gly Ala Gly Ala Glu Gly Ala Arg Leu Leu Ala Pro Pro Pro Gly 
                165                 170                 175     


Arg Cys Pro Arg Pro Ala Cys Pro Arg Ala Pro Gly Arg Leu Val Ser 
            180                 185                 190         


Ser Pro Gln Val Leu Ser Arg Leu Gly Val Ala Gly Gln Trp Arg Phe 
        195                 200                 205             


Val Asp Val Leu Gly Leu Glu Glu Glu Ser Leu Gly Ser Val Pro Ala 
    210                 215                 220                 


Pro Ala Cys Ala Leu Leu Leu Leu Phe Pro Leu Thr Ala Gln His Glu 
225                 230                 235                 240 


Asn Phe Arg Lys Lys Gln Ile Glu Glu Leu Lys Gly Gln Glu Val Ser 
                245                 250                 255     


Pro Lys Val Tyr Phe Met Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr 
            260                 265                 270         


Ile Gly Leu Ile His Ala Val Ala Asn Asn Gln Asp Lys Leu Gly Phe 
        275                 280                 285             


Glu Asp Gly Ser Val Leu Lys Gln Phe Leu Ser Glu Thr Glu Lys Met 
    290                 295                 300                 


Ser Pro Glu Asp Arg Ala Lys Cys Phe Glu Lys Asn Glu Ala Ile Gln 
305                 310                 315                 320 


Ala Ala His Asp Ala Val Ala Gln Glu Gly Gln Cys Arg Val Asp Asp 
                325                 330                 335     


Lys Val Asn Phe His Phe Ile Leu Phe Asn Asn Val Asp Gly His Leu 
            340                 345                 350         


Tyr Glu Leu Asp Gly Arg Met Pro Phe Pro Val Asn His Gly Ala Ser 
        355                 360                 365             


Ser Glu Asp Thr Leu Leu Lys Asp Ala Ala Lys Val Cys Arg Glu Phe 
    370                 375                 380                 


Thr Glu Arg Glu Gln Gly Glu Val Arg Phe Ser Ala Val Ala Leu Cys 
385                 390                 395                 400 


Lys Ala Ala 
            


<210>  6
<211>  223
<212>  PRT
<213>  Mus musculus

<400>  6

Met Gln Leu Lys Pro Met Glu Ile Asn Pro Glu Met Leu Asn Lys Val 
1               5                   10                  15      


Leu Ala Lys Leu Gly Val Ala Gly Gln Trp Arg Phe Ala Asp Val Leu 
            20                  25                  30          


Gly Leu Glu Glu Glu Thr Leu Gly Ser Val Pro Ser Pro Ala Cys Ala 
        35                  40                  45              


Leu Leu Leu Leu Phe Pro Leu Thr Ala Gln His Glu Asn Phe Arg Lys 
    50                  55                  60                  


Lys Gln Ile Glu Glu Leu Lys Gly Gln Glu Val Ser Pro Lys Val Tyr 
65                  70                  75                  80  


Phe Met Lys Gln Thr Ile Gly Asn Ser Cys Gly Thr Ile Gly Leu Ile 
                85                  90                  95      


His Ala Val Ala Asn Asn Gln Asp Lys Leu Glu Phe Glu Asp Gly Ser 
            100                 105                 110         


Val Leu Lys Gln Phe Leu Ser Glu Thr Glu Lys Leu Ser Pro Glu Asp 
        115                 120                 125             


Arg Ala Lys Cys Phe Glu Lys Asn Glu Ala Ile Gln Ala Ala His Asp 
    130                 135                 140                 


Ser Val Ala Gln Glu Gly Gln Cys Arg Val Asp Asp Lys Val Asn Phe 
145                 150                 155                 160 


His Phe Ile Leu Phe Asn Asn Val Asp Gly His Leu Tyr Glu Leu Asp 
                165                 170                 175     


Gly Arg Met Pro Phe Pro Val Asn His Gly Ala Ser Ser Glu Asp Ser 
            180                 185                 190         


Leu Leu Gln Asp Ala Ala Lys Val Cys Arg Glu Phe Thr Glu Arg Glu 
        195                 200                 205             


Gln Gly Glu Val Arg Phe Ser Ala Val Ala Leu Cys Lys Ala Ala 
    210                 215                 220             


<210>  7
<211>  7509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - pAAV-hUCHL1P-UCHL1-IRES-mCherry

<400>  7
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccgca cgcgttgaca ttgattattg actagttgga      180

gcccagttta gcagggttta cttctcagtt ttacttttca attgtgaccg taagcaaatt      240

aacttctcta ggcctgggat tctgatctgt aaaattgcac taatatgagt ctcttcacgg      300

ctcctctaag gattaaatga gagacacatg caaaggatcc ccaaaaacaa taactcaaaa      360

aatgttgatt ccctcccttc cctctgtcat ctgttaacct caacttccta aatagaaggt      420

ctattctttt accatcatca ttattctctt cggtgcctat ttttaaaaaa tactcaacct      480

tcttgcttcc ttcgctacct aagtatttct gcaagcccac tttgttctgc agcttagctt      540

tcctggcaca attcttatag attttggtcc cttttaaaat tcattcttca gcaaatgctt      600

tctctctcca tcttttgact agagatcatt agagatcacc tgagatcatt agagaacagt      660

ggtttccttg gttgccattc cctttcttct tcattgggag tattctgcgg tgaactcaga      720

cattttattt ttcaaagctt cccattcttt taaaaatgct tttcctttta cagcctctcg      780

ctcaaaatca tacccatctt ttccctggat ctgttttctc aagtctccaa tcgcctgcct      840

tctttgtgtc ttgtattacc ctcacatccc ccagctttct actgctctcc caggaccaac      900

catttcttcc gcgggagtca cattacatca gcattcctaa tgcagtatct gttatctacc      960

agattctgtt ttattctagg tagtcactta aaaacgaacc tcggtactgg tctgacttaa     1020

catggaggag gaattgtcta aggttaaacg caaactgctg agagatttgg ggcggggggc     1080

acacatttac attcattcgt attaaatata tacctgttga atttgtgctt tttctcaaat     1140

gcttcagaga ctcgagcttt agagtaattg ggatggtgaa aggatgggtt tccagaaact     1200

tcgcccaaaa ttaaagactc catcaaaagg actgctccat acactcaagg aacacccacc     1260

aacaaatccc gtctccacaa ccaccagatt atctcaccgg cgagtgagac tgcaaggttt     1320

gggggcccgg ccgtaccact ccgcgctgcg cacggggggt tcgtacccat ctggccgcga     1380

ccgtccgttt ccccctcgct tggttctgcc cctgctcccc ctgcacaggc ctcacagtgc     1440

gtctggccgg cgctttatag ctgcagcctg ggcgaccgga tcacaagttt gtacaaaaaa     1500

gcaggctccg cggccgcccc cttcaccatg cagctgaagc cgatggagat taaccccgag     1560

atgctgaaca aagtgttggc caagctgggg gtcgccggcc agtggcgctt cgccgacgtg     1620

ctagggctgg aggaggagac tctgggctca gtgccatccc ctgcctgcgc cctgctgctc     1680

ctgtttcccc tcacggccca gcatgaaaac ttcaggaaaa agcaaattga ggaactgaag     1740

ggacaggaag ttagccctaa agtttacttc atgaagcaga ccatcggaaa ctcctgtggt     1800

accatcgggt tgatccacgc agtggccaac aaccaagaca agctggaatt tgaggatgga     1860

tccgtcctga aacagtttct gtctgaaacg gagaagctgt cccccgaaga tagagccaag     1920

tgtttcgaga agaacgaggc catccaggca gcccatgact ccgtggccca ggagggccag     1980

tgtcgggtag atgacaaagt gaatttccat tttattctgt tcaacaacgt ggacggccat     2040

ctgtacgagc tcgatgggcg aatgcccttt ccagtgaacc atggcgccag ctcagaggac     2100

tctctgctgc aggatgctgc caaggtctgc agagaattca ctgagcgcga gcagggggag     2160

gtccgcttct ctgccgtggc tctctgcaaa gcagcttaaa tcagatccaa gggtgggcgc     2220

gccgacccag ctttcttgta caaagtggtg atccggtgct agcctcgaga attcacgcgt     2280

cgagcatgca tctagggcgg ccaattccgc ccctctccct cccccccccc taacgttact     2340

ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgtgatt ttccaccata     2400

ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt     2460

cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa     2520

gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag     2580

cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca     2640

cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc     2700

aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat     2760

tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta     2820

aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga     2880

taagcttgcc acaacccggg atccaccatg gtgagcaagg gcgaggagga taacatggcc     2940

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag     3000

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg     3060

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg     3120

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc     3180

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc     3240

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc     3300

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc     3360

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag     3420

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc     3480

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag     3540

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg     3600

gacgagctgt acaagtaatg aattcgctta tcgataatca acctctggat tacaaaattt     3660

gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg     3720

ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt     3780

ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg     3840

tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc     3900

agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg     3960

cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt     4020

tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc     4080

gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg     4140

gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga     4200

tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg     4260

gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc     4320

caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta     4380

taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg     4440

tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac     4500

tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg     4560

attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt     4620

caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc     4680

tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt     4740

gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact     4800

ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg     4860

ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg     4920

atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac     4980

catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     5040

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     5100

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     5160

gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta     5220

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     5280

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg     5340

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     5400

aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta     5460

caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg     5520

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg     5580

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc     5640

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag     5700

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     5760

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     5820

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     5880

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     5940

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     6000

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg     6060

tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga     6120

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     6180

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     6240

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     6300

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     6360

ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     6420

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     6480

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     6540

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     6600

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     6660

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     6720

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     6780

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     6840

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     6900

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     6960

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     7020

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     7080

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     7140

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     7200

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     7260

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     7320

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     7380

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     7440

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     7500

ctcacatgt                                                             7509


<210>  8
<211>  8848
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic -pAAV-CBA-UCHL1-IRES-mCherry

<400>  8
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccgca cgcgttgaca ttgattattg actagttatt      180

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat      240

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa      300

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg      360

actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc      420

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct      480

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatgggtc      540

gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt      600

ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg      660

cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc      720

ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg      780

gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg      840

tgccccgctc cgcgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc      900

acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg      960

acggctcgtt tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt     1020

gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt     1080

gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct     1140

ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg     1200

aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg     1260

cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg     1320

gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg     1380

tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg     1440

gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt     1500

gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctggcgga     1560

gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc     1620

gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt     1680

ctccatctcc agcctcgggg ctgccgcagg gggacggctg ccttcggggg ggacggggca     1740

gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc     1800

atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc     1860

attttggcaa agaattctgc agtcgaatca caagtttgta caaaaaagct gaacgagaaa     1920

cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat     1980

aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt     2040

tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt     2100

ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat     2160

atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta     2220

taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca     2280

caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt     2340

ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac     2400

cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt     2460

ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta     2520

tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt     2580

caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat     2640

gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca     2700

tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga     2760

tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta     2820

tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag     2880

tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc     2940

tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc     3000

agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg     3060

ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt     3120

gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat     3180

gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca     3240

cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa     3300

agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa     3360

gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg     3420

ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg     3480

gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat     3540

attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatcta     3600

gcctcgagaa ttcacgcgtc gagcatgcat ctagggcggc caattccgcc cctctccctc     3660

ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta     3720

tatgtgattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc     3780

tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct     3840

gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt     3900

agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa     3960

gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg     4020

gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga     4080

tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac     4140

atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt     4200

cctttgaaaa acacgatgat aagcttgcca caacccggga tccaccatgg tgagcaaggg     4260

cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg     4320

ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg     4380

cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat     4440

cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc     4500

cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga     4560

ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta     4620

caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac     4680

catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga     4740

gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac     4800

ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga     4860

catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg     4920

ccactccacc ggcggcatgg acgagctgta caagtaatga attcgcttat cgataatcaa     4980

cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt     5040

acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct     5100

ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc     5160

gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg     5220

ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc     5280

acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc     5340

actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt     5400

gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca     5460

gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt     5520

cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg     5580

ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg     5640

aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt     5700

ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc     5760

aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag     5820

tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc     5880

agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt     5940

tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg     6000

tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct     6060

tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct     6120

agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc     6180

aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag     6240

ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca     6300

ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt     6360

gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc     6420

gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg     6480

gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat     6540

ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg     6600

ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct     6660

atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa     6720

aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt     6780

ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac     6840

ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga     6900

caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa     6960

cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata     7020

atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt     7080

ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg     7140

cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt     7200

cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta     7260

aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc     7320

ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa     7380

gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc     7440

cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt     7500

acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact     7560

gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac     7620

aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata     7680

ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta     7740

ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg     7800

gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat     7860

aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt     7920

aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga     7980

aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa     8040

gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     8100

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     8160

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     8220

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     8280

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     8340

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     8400

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     8460

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     8520

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     8580

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     8640

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     8700

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     8760

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     8820

gccttttgct ggccttttgc tcacatgt                                        8848


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype Uchl1nm3419 mice

<400>  9
tggacggctg tgtgtgctaa tg                                                22


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype Uchl1nm3419 mice

<400>  10
ctaagggaag ggtcttgctc atc                                               23


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype Uchl1nm3419 mice

<400>  11
gtcatctacc tgaagagagc caag                                              24


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype floxed UCHL1 mice

<400>  12
tagtccaatc cttgtaccag ttgg                                              24


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype floxed UCHL1 mice

<400>  13
ccatggttct agatgctgtt gaatgc                                            26


<210>  14
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype cre mice

<400>  14
gcattaccgg tcgatgcaac gagtgat                                           27


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer used to genotype cre mice

<400>  15
gagtgaacga acctggtcga aatcagt                                           27


