﻿               SEQUENCE LISTING

<110> Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

<120> Novel genetically engineered microorganism capable of growing on formate, methanol, methane or CO2

<130> AC2850 PCT S3

<150> EP 19 21 4725.4
<151> 2019-12-10

<160> 62

<170> BiSSAP 1.3

<210> 1
<211> 557
<212> PRT
<213> Methylobacterium extorquens

<220> 
<223> Formate-tetrahydrofolate ligase (EC:6.3.4.3)

<400> 1
Met Pro Ser Asp Ile Glu Ile Ala Arg Ala Ala Thr Leu Lys Pro Ile 
1               5                   10                  15      
Ala Gln Val Ala Glu Lys Leu Gly Ile Pro Asp Glu Ala Leu His Asn 
            20                  25                  30          
Tyr Gly Lys His Ile Ala Lys Ile Asp His Asp Phe Ile Ala Ser Leu 
        35                  40                  45              
Glu Gly Lys Pro Glu Gly Lys Leu Val Leu Val Thr Ala Ile Ser Pro 
    50                  55                  60                  
Thr Pro Ala Gly Glu Gly Lys Thr Thr Thr Thr Val Gly Leu Gly Asp 
65                  70                  75                  80  
Ala Leu Asn Arg Ile Gly Lys Arg Ala Val Met Cys Leu Arg Glu Pro 
                85                  90                  95      
Ser Leu Gly Pro Cys Phe Gly Met Lys Gly Gly Ala Ala Gly Gly Gly 
            100                 105                 110         
Lys Ala Gln Val Val Pro Met Glu Gln Ile Asn Leu His Phe Thr Gly 
        115                 120                 125             
Asp Phe His Ala Ile Thr Ser Ala His Ser Leu Ala Ala Ala Leu Ile 
    130                 135                 140                 
Asp Asn His Ile Tyr Trp Ala Asn Glu Leu Asn Ile Asp Val Arg Arg 
145                 150                 155                 160 
Ile His Trp Arg Arg Val Val Asp Met Asn Asp Arg Ala Leu Arg Ala 
                165                 170                 175     
Ile Asn Gln Ser Leu Gly Gly Val Ala Asn Gly Phe Pro Arg Glu Asp 
            180                 185                 190         
Gly Phe Asp Ile Thr Val Ala Ser Glu Val Met Ala Val Phe Cys Leu 
        195                 200                 205             
Ala Lys Asn Leu Ala Asp Leu Glu Glu Arg Leu Gly Arg Ile Val Ile 
    210                 215                 220                 
Ala Glu Thr Arg Asp Arg Lys Pro Val Thr Leu Ala Asp Val Lys Ala 
225                 230                 235                 240 
Thr Gly Ala Met Thr Val Leu Leu Lys Asp Ala Leu Gln Pro Asn Leu 
                245                 250                 255     
Val Gln Thr Leu Glu Gly Asn Pro Ala Leu Ile His Gly Gly Pro Phe 
            260                 265                 270         
Ala Asn Ile Ala His Gly Cys Asn Ser Val Ile Ala Thr Arg Thr Gly 
        275                 280                 285             
Leu Arg Leu Ala Asp Tyr Thr Val Thr Glu Ala Gly Phe Gly Ala Asp 
    290                 295                 300                 
Leu Gly Ala Glu Lys Phe Ile Asp Ile Lys Cys Arg Gln Thr Gly Leu 
305                 310                 315                 320 
Lys Pro Ser Ala Val Val Ile Val Ala Thr Ile Arg Ala Leu Lys Met 
                325                 330                 335     
His Gly Gly Val Asn Lys Lys Asp Leu Gln Ala Glu Asn Leu Asp Ala 
            340                 345                 350         
Leu Glu Lys Gly Phe Ala Asn Leu Glu Arg His Val Asn Asn Val Arg 
        355                 360                 365             
Ser Phe Gly Leu Pro Val Val Val Gly Val Asn His Phe Phe Gln Asp 
    370                 375                 380                 
Thr Asp Ala Glu His Ala Arg Leu Lys Glu Leu Cys Arg Asp Arg Leu 
385                 390                 395                 400 
Gln Val Glu Ala Ile Thr Cys Lys His Trp Ala Glu Gly Gly Ala Gly 
                405                 410                 415     
Ala Glu Ala Leu Ala Gln Ala Val Val Lys Leu Ala Glu Gly Glu Gln 
            420                 425                 430         
Lys Pro Leu Thr Phe Ala Tyr Glu Thr Glu Thr Lys Ile Thr Asp Lys 
        435                 440                 445             
Ile Lys Ala Ile Ala Thr Lys Leu Tyr Gly Ala Ala Asp Ile Gln Ile 
    450                 455                 460                 
Glu Ser Lys Ala Ala Thr Lys Leu Ala Gly Phe Glu Lys Asp Gly Tyr 
465                 470                 475                 480 
Gly Gly Leu Pro Val Cys Met Ala Lys Thr Gln Tyr Ser Phe Ser Thr 
                485                 490                 495     
Asp Pro Thr Leu Met Gly Ala Pro Ser Gly His Leu Val Ser Val Arg 
            500                 505                 510         
Asp Val Arg Leu Ser Ala Gly Ala Gly Phe Val Val Val Ile Cys Gly 
        515                 520                 525             
Glu Ile Met Thr Met Pro Gly Leu Pro Lys Val Pro Ala Ala Asp Thr 
    530                 535                 540                 
Ile Arg Leu Asp Ala Asn Gly Gln Ile Asp Gly Leu Phe 
545                 550                 555         

<210> 2
<211> 1674
<212> DNA
<213> Methylobacterium extorquens

<220> 
<223> Formate-tetrahydrofolate ligase (EC:6.3.4.3)

<400> 2
atgccgtctg acatcgagat cgctcgtgct gctaccctga agccgatcgc tcaggttgct      60

gagaagctgg gtatcccgga cgaagctctg cacaactacg gtaaacacat cgctaaaatc     120

gaccacgact tcatcgcttc tctggaaggt aaaccggaag gtaaactggt tctggttacc     180

gctatctctc cgaccccggc tggtgaaggt aaaaccacca ccaccgttgg tctgggtgac     240

gctctgaacc gtatcggtaa acgtgctgtt atgtgcctgc gtgaaccgtc tctgggtccg     300

tgctttggta tgaagggtgg tgctgctggt ggtggtaaag ctcaggttgt tccgatggaa     360

cagatcaacc tgcacttcac cggtgacttc cacgctatca cctctgctca ctctctggct     420

gctgctctga tcgacaacca catctactgg gctaacgaac tgaacatcga cgtgcgtcgt     480

atccactggc gtcgtgttgt tgacatgaac gaccgtgctc tgcgtgctat caaccagtct     540

ctgggtggtg ttgctaacgg tttcccgcgt gaagacggtt ttgacatcac cgttgcttct     600

gaggttatgg ctgtgttctg cctggccaaa aacctggctg acctggaaga acgtctgggt     660

cgtatcgtta tcgctgagac ccgtgaccgt aaaccggtta ccctggctga cgttaaagct     720

accggtgcta tgaccgttct gctgaaggac gctctgcaac cgaacctggt tcagaccctg     780

gaaggtaacc cggctctgat ccacggtggt ccgtttgcta acatcgctca cggttgcaac     840

tctgttatcg ctacccgtac cggtctgcgt ctggctgact acaccgttac cgaagctggt     900

tttggtgctg acctgggtgc tgagaagttc atcgacatca aatgccgtca aactggtctg     960

aagccgtctg ctgttgttat cgttgctacc atccgtgctc tgaagatgca cggtggtgtt    1020

aacaaaaaag acctgcaagc tgagaacctg gacgctctgg agaagggttt tgctaacctg    1080

gaacgtcacg ttaacaacgt gcgttctttt ggtctgccgg ttgttgttgg tgttaaccac    1140

ttcttccagg acaccgacgc tgaacacgct cgtctgaagg aactgtgccg tgaccgtctg    1200

caagttgaag ctatcacctg caaacactgg gctgaaggtg gtgctggtgc tgaagctctg    1260

gctcaggctg ttgttaaact ggctgaaggt gaacagaagc cgctgacctt tgcttacgag    1320

accgagacca aaatcaccga caaaatcaaa gctatcgcta ccaaactgta cggtgctgct    1380

gacatccaga tcgaatctaa agctgctacc aaactggctg gttttgagaa ggacggttac    1440

ggtggcctgc cggtctgcat ggcaaaaacc cagtactctt tctctaccga cccgaccctg    1500

atgggtgctc cgtctggcca cctggtgagc gtgcgtgacg tgcgtctgtc tgctggtgct    1560

ggttttgttg ttgttatctg cggtgagatc atgaccatgc cgggtctgcc gaaggttccg    1620

gctgctgaca ccatccgtct ggacgctaac ggtcagatcg acggtctgtt ctaa          1674


<210> 3
<211> 208
<212> PRT
<213> Methylobacterium extorquens

<220> 
<223> Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)

<400> 3
Met Ala Gly Asn Glu Thr Ile Glu Thr Phe Leu Asp Gly Leu Ala Ser 
1               5                   10                  15      
Ser Ala Pro Thr Pro Gly Gly Gly Gly Ala Ala Ala Ile Ser Gly Ala 
            20                  25                  30          
Met Gly Ala Ala Leu Val Ser Met Val Cys Asn Leu Thr Ile Gly Lys 
        35                  40                  45              
Lys Lys Tyr Val Glu Val Glu Ala Asp Leu Lys Gln Val Leu Glu Lys 
    50                  55                  60                  
Ser Glu Gly Leu Arg Arg Thr Leu Thr Gly Met Ile Ala Asp Asp Val 
65                  70                  75                  80  
Glu Ala Phe Asp Ala Val Met Gly Ala Tyr Gly Leu Pro Lys Asn Thr 
                85                  90                  95      
Asp Glu Glu Lys Ala Ala Arg Ala Ala Lys Ile Gln Glu Ala Leu Lys 
            100                 105                 110         
Thr Ala Thr Asp Val Pro Leu Ala Cys Cys Arg Val Cys Arg Glu Val 
        115                 120                 125             
Ile Asp Leu Ala Glu Ile Val Ala Glu Lys Gly Asn Leu Asn Val Ile 
    130                 135                 140                 
Ser Asp Ala Gly Val Ala Val Leu Ser Ala Tyr Ala Gly Leu Arg Ser 
145                 150                 155                 160 
Ala Ala Leu Asn Val Tyr Val Asn Ala Lys Gly Leu Asp Asp Arg Ala 
                165                 170                 175     
Phe Ala Glu Glu Arg Leu Lys Glu Leu Glu Gly Leu Leu Ala Glu Ala 
            180                 185                 190         
Gly Ala Leu Asn Glu Arg Ile Tyr Glu Thr Val Lys Ser Lys Val Asn 
        195                 200                 205             



<210> 4
<211> 627
<212> DNA
<213> Methylobacterium extorquens

<220> 
<223> Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)

<400> 4
atggctggta acgaaaccat cgaaaccttc ctggacggtc tggcttcttc tgctccgacc      60

ccgggtggtg gtggtgctgc tgctatctct ggtgctatgg gtgctgctct ggtttctatg     120

gtttgcaacc tgaccatcgg taaaaaaaaa tacgttgaag ttgaagctga cctgaaacag     180

gttctggaaa aatctgaagg tctgcgtcgt accctgaccg gtatgatcgc tgacgacgtt     240

gaagctttcg acgctgttat gggtgcttac ggtctgccga aaaacaccga cgaagaaaaa     300

gctgctcgtg ctgctaaaat ccaggaagct ctgaaaaccg ctaccgacgt tccgctggct     360

tgctgccgtg tttgccgtga agttatcgac ctggctgaaa tcgttgctga aaaaggtaac     420

ctgaacgtta tctctgacgc tggtgttgct gttctgtctg cttacgctgg tctgcgttct     480

gctgctctga acgtttacgt taacgctaaa ggtctggacg accgtgcttt cgctgaagaa     540

cgtctgaaag aactggaagg tctgctggct gaagctggtg ctctgaacga acgtatctac     600

gaaaccgtta aatctaaagt taactaa                                         627


<210> 5
<211> 288
<212> PRT
<213> Methylobacterium extorquens

<220> 
<223> Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)

<400> 5
Met Ser Lys Lys Leu Leu Phe Gln Phe Asp Thr Asp Ala Thr Pro Ser 
1               5                   10                  15      
Val Phe Asp Val Val Val Gly Tyr Asp Gly Gly Ala Asp His Ile Thr 
            20                  25                  30          
Gly Tyr Gly Asn Val Thr Pro Asp Asn Val Gly Ala Tyr Val Asp Gly 
        35                  40                  45              
Thr Ile Tyr Thr Arg Gly Gly Lys Glu Lys Gln Ser Thr Ala Ile Phe 
    50                  55                  60                  
Val Gly Gly Gly Asp Met Ala Ala Gly Glu Arg Val Phe Glu Ala Val 
65                  70                  75                  80  
Lys Lys Arg Phe Phe Gly Pro Phe Arg Val Ser Cys Met Leu Asp Ser 
                85                  90                  95      
Asn Gly Ser Asn Thr Thr Ala Ala Ala Gly Val Ala Leu Val Val Lys 
            100                 105                 110         
Ala Ala Gly Gly Ser Val Lys Gly Lys Lys Ala Val Val Leu Ala Gly 
        115                 120                 125             
Thr Gly Pro Val Gly Met Arg Ser Ala Ala Leu Leu Ala Gly Glu Gly 
    130                 135                 140                 
Ala Glu Val Val Leu Cys Gly Arg Lys Leu Asp Lys Ala Gln Ala Ala 
145                 150                 155                 160 
Ala Asp Ser Val Asn Lys Arg Phe Lys Val Asn Val Thr Ala Ala Glu 
                165                 170                 175     
Thr Ala Asp Asp Ala Ser Arg Ala Glu Ala Val Lys Gly Ala His Phe 
            180                 185                 190         
Val Phe Thr Ala Gly Ala Ile Gly Leu Glu Leu Leu Pro Gln Ala Ala 
        195                 200                 205             
Trp Gln Asn Glu Ser Ser Ile Glu Ile Val Ala Asp Tyr Asn Ala Gln 
    210                 215                 220                 
Pro Pro Leu Gly Ile Gly Gly Ile Asp Ala Thr Asp Lys Gly Lys Glu 
225                 230                 235                 240 
Tyr Gly Gly Lys Arg Ala Phe Gly Ala Leu Gly Ile Gly Gly Leu Lys 
                245                 250                 255     
Leu Lys Leu His Arg Ala Cys Ile Ala Lys Leu Phe Glu Ser Ser Glu 
            260                 265                 270         
Gly Val Phe Asp Ala Glu Glu Ile Tyr Lys Leu Ala Lys Glu Met Ala 
        275                 280                 285             



<210> 6
<211> 867
<212> DNA
<213> Methylobacterium extorquens

<220> 
<223> Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)

<400> 6
atgtctaaaa aactgctgtt ccagttcgac accgacgcta ccccgtctgt tttcgacgtt      60

gttgttggtt acgacggtgg tgctgaccac atcaccggtt acggtaacgt taccccggac     120

aacgttggtg cttacgttga cggtaccatc tacacccgtg gtggtaaaga aaaacagtct     180

accgctatct tcgttggtgg tggtgacatg gctgctggtg aacgtgtttt cgaagctgtt     240

aaaaaacgtt tcttcggtcc gttccgtgtt tcttgcatgc tggactctaa cggttctaac     300

accaccgctg ctgctggtgt tgctctggtt gttaaagctg ctggtggttc tgttaaaggt     360

aaaaaagctg ttgttctggc tggtaccggt ccggttggta tgcgttctgc tgctctgctg     420

gctggtgaag gtgctgaagt tgttctgtgc ggtcgtaaac tggacaaagc tcaggctgct     480

gctgactctg ttaacaaacg tttcaaagtt aacgttaccg ctgctgaaac cgctgacgac     540

gcttctcgtg ctgaagctgt taaaggtgct cacttcgttt tcaccgctgg tgctatcggt     600

ctggaactgc tgccgcaggc tgcttggcag aacgaatctt ctatcgaaat cgttgctgac     660

tacaacgctc agccgccgct gggtatcggt ggtatcgacg ctaccgacaa aggtaaagaa     720

tacggtggta aacgtgcttt cggtgctctg ggtatcggtg gtctgaaact gaaactgcac     780

cgtgcttgca tcgctaaact gttcgaatct tctgaaggtg ttttcgacgc tgaagaaatc     840

tacaaactgg ctaaagaaat ggcttaa                                         867


<210> 7
<211> 364
<212> PRT
<213> Escherichia coli

<220> 
<223> Aminomethyltransferase (EC:2.2.2.10)

<400> 7
Met Ala Gln Gln Thr Pro Leu Tyr Glu Gln His Thr Leu Cys Gly Ala 
1               5                   10                  15      
Arg Met Val Asp Phe His Gly Trp Met Met Pro Leu His Tyr Gly Ser 
            20                  25                  30          
Gln Ile Asp Glu His His Ala Val Arg Thr Asp Ala Gly Met Phe Asp 
        35                  40                  45              
Val Ser His Met Thr Ile Val Asp Leu Arg Gly Ser Arg Thr Arg Glu 
    50                  55                  60                  
Phe Leu Arg Tyr Leu Leu Ala Asn Asp Val Ala Lys Leu Thr Lys Ser 
65                  70                  75                  80  
Gly Lys Ala Leu Tyr Ser Gly Met Leu Asn Ala Ser Gly Gly Val Ile 
                85                  90                  95      
Asp Asp Leu Ile Val Tyr Tyr Phe Thr Glu Asp Phe Phe Arg Leu Val 
            100                 105                 110         
Val Asn Ser Ala Thr Arg Glu Lys Asp Leu Ser Trp Ile Thr Gln His 
        115                 120                 125             
Ala Glu Pro Phe Gly Ile Glu Ile Thr Val Arg Asp Asp Leu Ser Met 
    130                 135                 140                 
Ile Ala Val Gln Gly Pro Asn Ala Gln Ala Lys Ala Ala Thr Leu Phe 
145                 150                 155                 160 
Asn Asp Ala Gln Arg Gln Ala Val Glu Gly Met Lys Pro Phe Phe Gly 
                165                 170                 175     
Val Gln Ala Gly Asp Leu Phe Ile Ala Thr Thr Gly Tyr Thr Gly Glu 
            180                 185                 190         
Ala Gly Tyr Glu Ile Ala Leu Pro Asn Glu Lys Ala Ala Asp Phe Trp 
        195                 200                 205             
Arg Ala Leu Val Glu Ala Gly Val Lys Pro Cys Gly Leu Gly Ala Arg 
    210                 215                 220                 
Asp Thr Leu Arg Leu Glu Ala Gly Met Asn Leu Tyr Gly Gln Glu Met 
225                 230                 235                 240 
Asp Glu Thr Ile Ser Pro Leu Ala Ala Asn Met Gly Trp Thr Ile Ala 
                245                 250                 255     
Trp Glu Pro Ala Asp Arg Asp Phe Ile Gly Arg Glu Ala Leu Glu Val 
            260                 265                 270         
Gln Arg Glu His Gly Thr Glu Lys Leu Val Gly Leu Val Met Thr Glu 
        275                 280                 285             
Lys Gly Val Leu Arg Asn Glu Leu Pro Val Arg Phe Thr Asp Ala Gln 
    290                 295                 300                 
Gly Asn Gln His Glu Gly Ile Ile Thr Ser Gly Thr Phe Ser Pro Thr 
305                 310                 315                 320 
Leu Gly Tyr Ser Ile Ala Leu Ala Arg Val Pro Glu Gly Ile Gly Glu 
                325                 330                 335     
Thr Ala Ile Val Gln Ile Arg Asn Arg Glu Met Pro Val Lys Val Thr 
            340                 345                 350         
Lys Pro Val Phe Val Arg Asn Gly Lys Ala Val Ala 
        355                 360                 

<210> 8
<211> 1095
<212> DNA
<213> Escherichia coli

<220> 
<223> Aminomethyltransferase (EC:2.2.2.10)

<400> 8
atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat      60

ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta     120

cgtaccgatg ccggaatgtt tgatgtgtca catatgacca tcgtcgatct tcgcggcagc     180

cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc     240

ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga tgacctcatc     300

gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa     360

gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat     420

gacctttcca tgattgccgt gcaagggccg aatgcgcagg caaaagctgc cacactgttt     480

aatgacgccc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggcgggc     540

gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc     600

aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag cgggtgttaa gccatgtggc     660

ttgggcgcgc gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg     720

gacgaaacca tctctccttt agccgccaac atgggctgga ccatcgcctg ggaaccggca     780

gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa     840

ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt     900

accgatgcgc agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg     960

ctgggttaca gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg    1020

caaattcgca accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc    1080

aaagccgtcg cgtga                                                     1095


<210> 9
<211> 129
<212> PRT
<213> Escherichia coli

<220> 
<223> Glycine cleavage system H protein

<400> 9
Met Ser Asn Val Pro Ala Glu Leu Lys Tyr Ser Lys Glu His Glu Trp 
1               5                   10                  15      
Leu Arg Lys Glu Ala Asp Gly Thr Tyr Thr Val Gly Ile Thr Glu His 
            20                  25                  30          
Ala Gln Glu Leu Leu Gly Asp Met Val Phe Val Asp Leu Pro Glu Val 
        35                  40                  45              
Gly Ala Thr Val Ser Ala Gly Asp Asp Cys Ala Val Ala Glu Ser Val 
    50                  55                  60                  
Lys Ala Ala Ser Asp Ile Tyr Ala Pro Val Ser Gly Glu Ile Val Ala 
65                  70                  75                  80  
Val Asn Asp Ala Leu Ser Asp Ser Pro Glu Leu Val Asn Ser Glu Pro 
                85                  90                  95      
Tyr Ala Gly Gly Trp Ile Phe Lys Ile Lys Ala Ser Asp Glu Ser Glu 
            100                 105                 110         
Leu Glu Ser Leu Leu Asp Ala Thr Ala Tyr Glu Ala Leu Leu Glu Asp 
        115                 120                 125             
Glu 
    

<210> 10
<211> 390
<212> DNA
<213> Escherichia coli

<220> 
<223> Glycine cleavage system H protein

<400> 10
atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa      60

gccgacggca cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg     120

gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt     180

gccgaatcgg taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg     240

gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc     300

tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc     360

gcatacgaag cattgttaga agacgagtaa                                      390


<210> 11
<211> 957
<212> PRT
<213> Escherichia coli

<220> 
<223> Glycine dehydrogenase (decarboxylating) (EC:1.4.4.2)

<400> 11
Met Thr Gln Thr Leu Ser Gln Leu Glu Asn Ser Gly Ala Phe Ile Glu 
1               5                   10                  15      
Arg His Ile Gly Pro Asp Ala Ala Gln Gln Gln Glu Met Leu Asn Ala 
            20                  25                  30          
Val Gly Ala Gln Ser Leu Asn Ala Leu Thr Gly Gln Ile Val Pro Lys 
        35                  40                  45              
Asp Ile Gln Leu Ala Thr Pro Pro Gln Val Gly Ala Pro Ala Thr Glu 
    50                  55                  60                  
Tyr Ala Ala Leu Ala Glu Leu Lys Ala Ile Ala Ser Arg Asn Lys Arg 
65                  70                  75                  80  
Phe Thr Ser Tyr Ile Gly Met Gly Tyr Thr Ala Val Gln Leu Pro Pro 
                85                  90                  95      
Val Ile Leu Arg Asn Met Leu Glu Asn Pro Gly Trp Tyr Thr Ala Tyr 
            100                 105                 110         
Thr Pro Tyr Gln Pro Glu Val Ser Gln Gly Arg Leu Glu Ala Leu Leu 
        115                 120                 125             
Asn Phe Gln Gln Val Thr Leu Asp Leu Thr Gly Leu Asp Met Ala Ser 
    130                 135                 140                 
Ala Ser Leu Leu Asp Glu Ala Thr Ala Ala Ala Glu Ala Met Ala Met 
145                 150                 155                 160 
Ala Lys Arg Val Ser Lys Leu Lys Asn Ala Asn Arg Phe Phe Val Ala 
                165                 170                 175     
Ser Asp Val His Pro Gln Thr Leu Asp Val Val Arg Thr Arg Ala Glu 
            180                 185                 190         
Thr Phe Gly Phe Glu Val Ile Val Asp Asp Ala Gln Lys Val Leu Asp 
        195                 200                 205             
His Gln Asp Val Phe Gly Val Leu Leu Gln Gln Val Gly Thr Thr Gly 
    210                 215                 220                 
Glu Ile His Asp Tyr Thr Ala Leu Ile Ser Glu Leu Lys Ser Arg Lys 
225                 230                 235                 240 
Ile Val Val Ser Val Ala Ala Asp Ile Met Ala Leu Val Leu Leu Thr 
                245                 250                 255     
Ala Pro Gly Lys Gln Gly Ala Asp Ile Val Phe Gly Ser Ala Gln Arg 
            260                 265                 270         
Phe Gly Val Pro Met Gly Tyr Gly Gly Pro His Ala Ala Phe Phe Ala 
        275                 280                 285             
Ala Lys Asp Glu Tyr Lys Arg Ser Met Pro Gly Arg Ile Ile Gly Val 
    290                 295                 300                 
Ser Lys Asp Ala Ala Gly Asn Thr Ala Leu Arg Met Ala Met Gln Thr 
305                 310                 315                 320 
Arg Glu Gln His Ile Arg Arg Glu Lys Ala Asn Ser Asn Ile Cys Thr 
                325                 330                 335     
Ser Gln Val Leu Leu Ala Asn Ile Ala Ser Leu Tyr Ala Val Tyr His 
            340                 345                 350         
Gly Pro Val Gly Leu Lys Arg Ile Ala Asn Arg Ile His Arg Leu Thr 
        355                 360                 365             
Asp Ile Leu Ala Ala Gly Leu Gln Gln Lys Gly Leu Lys Leu Arg His 
    370                 375                 380                 
Ala His Tyr Phe Asp Thr Leu Cys Val Glu Val Ala Asp Lys Ala Gly 
385                 390                 395                 400 
Val Leu Thr Arg Ala Glu Ala Ala Glu Ile Asn Leu Arg Ser Asp Ile 
                405                 410                 415     
Leu Asn Ala Val Gly Ile Thr Leu Asp Glu Thr Thr Thr Arg Glu Asn 
            420                 425                 430         
Val Met Gln Leu Phe Asn Val Leu Leu Gly Asp Asn His Gly Leu Asp 
        435                 440                 445             
Ile Asp Thr Leu Asp Lys Asp Val Ala His Asp Ser Arg Ser Ile Gln 
    450                 455                 460                 
Pro Ala Met Leu Arg Asp Asp Glu Ile Leu Thr His Pro Val Phe Asn 
465                 470                 475                 480 
Arg Tyr His Ser Glu Thr Glu Met Met Arg Tyr Met His Ser Leu Glu 
                485                 490                 495     
Arg Lys Asp Leu Ala Leu Asn Gln Ala Met Ile Pro Leu Gly Ser Cys 
            500                 505                 510         
Thr Met Lys Leu Asn Ala Ala Ala Glu Met Ile Pro Ile Thr Trp Pro 
        515                 520                 525             
Glu Phe Ala Glu Leu His Pro Phe Cys Pro Pro Glu Gln Ala Glu Gly 
    530                 535                 540                 
Tyr Gln Gln Met Ile Ala Gln Leu Ala Asp Trp Leu Val Lys Leu Thr 
545                 550                 555                 560 
Gly Tyr Asp Ala Val Cys Met Gln Pro Asn Ser Gly Ala Gln Gly Glu 
                565                 570                 575     
Tyr Ala Gly Leu Leu Ala Ile Arg His Tyr His Glu Ser Arg Asn Glu 
            580                 585                 590         
Gly His Arg Asp Ile Cys Leu Ile Pro Ala Ser Ala His Gly Thr Asn 
        595                 600                 605             
Pro Ala Ser Ala His Met Ala Gly Met Gln Val Val Val Val Ala Cys 
    610                 615                 620                 
Asp Lys Asn Gly Asn Ile Asp Leu Thr Asp Leu Arg Ala Lys Ala Glu 
625                 630                 635                 640 
Gln Ala Gly Asp Asn Leu Ser Cys Ile Met Val Thr Tyr Pro Ser Thr 
                645                 650                 655     
His Gly Val Tyr Glu Glu Thr Ile Arg Glu Val Cys Glu Val Val His 
            660                 665                 670         
Gln Phe Gly Gly Gln Val Tyr Leu Asp Gly Ala Asn Met Asn Ala Gln 
        675                 680                 685             
Val Gly Ile Thr Ser Pro Gly Phe Ile Gly Ala Asp Val Ser His Leu 
    690                 695                 700                 
Asn Leu His Lys Thr Phe Cys Ile Pro His Gly Gly Gly Gly Pro Gly 
705                 710                 715                 720 
Met Gly Pro Ile Gly Val Lys Ala His Leu Ala Pro Phe Val Pro Gly 
                725                 730                 735     
His Ser Val Val Gln Ile Glu Gly Met Leu Thr Arg Gln Gly Ala Val 
            740                 745                 750         
Ser Ala Ala Pro Phe Gly Ser Ala Ser Ile Leu Pro Ile Ser Trp Met 
        755                 760                 765             
Tyr Ile Arg Met Met Gly Ala Glu Gly Leu Lys Lys Ala Ser Gln Val 
    770                 775                 780                 
Ala Ile Leu Asn Ala Asn Tyr Ile Ala Ser Arg Leu Gln Asp Ala Phe 
785                 790                 795                 800 
Pro Val Leu Tyr Thr Gly Arg Asp Gly Arg Val Ala His Glu Cys Ile 
                805                 810                 815     
Leu Asp Ile Arg Pro Leu Lys Glu Glu Thr Gly Ile Ser Glu Leu Asp 
            820                 825                 830         
Ile Ala Lys Arg Leu Ile Asp Tyr Gly Phe His Ala Pro Thr Met Ser 
        835                 840                 845             
Phe Pro Val Ala Gly Thr Leu Met Val Glu Pro Thr Glu Ser Glu Ser 
    850                 855                 860                 
Lys Val Glu Leu Asp Arg Phe Ile Asp Ala Met Leu Ala Ile Arg Ala 
865                 870                 875                 880 
Glu Ile Asp Gln Val Lys Ala Gly Val Trp Pro Leu Glu Asp Asn Pro 
                885                 890                 895     
Leu Val Asn Ala Pro His Ile Gln Ser Glu Leu Val Ala Glu Trp Ala 
            900                 905                 910         
His Pro Tyr Ser Arg Glu Val Ala Val Phe Pro Ala Gly Val Ala Asp 
        915                 920                 925             
Lys Tyr Trp Pro Thr Val Lys Arg Leu Asp Asp Val Tyr Gly Asp Arg 
    930                 935                 940                 
Asn Leu Phe Cys Ser Cys Val Pro Ile Ser Glu Tyr Gln 
945                 950                 955         

<210> 12
<211> 2874
<212> DNA
<213> Escherichia coli

<220> 
<223> Glycine dehydrogenase (decarboxylating) (EC:1.4.4.2)

<400> 12
atgacacaga cgttaagcca gcttgaaaac agcggcgctt ttattgaacg ccatatcgga      60

ccggacgccg cgcaacagca agaaatgctg aatgccgttg gtgcacaatc gttaaacgcg     120

ctgaccggcc agattgtgcc gaaagatatt caacttgcga caccaccgca ggttggcgca     180

ccggcgaccg aatacgccgc actggcagaa ctcaaggcta ttgccagtcg caataaacgc     240

ttcacgtctt acatcggcat gggttacacc gccgtgcagc taccgccggt tatcctgcgt     300

aacatgctgg aaaatccggg ctggtatacc gcgtacactc cgtatcaacc tgaagtctcc     360

cagggccgcc ttgaagcact gctcaacttc cagcaggtaa cgctggattt gactggactg     420

gatatggcct ctgcttctct tctggacgag gccaccgctg ccgccgaagc aatggcgatg     480

gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc cgatgtgcat     540

ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga agtgattgtc     600

gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggta     660

ggcactaccg gtgaaattca cgactacact gcgcttatta gcgaactgaa atcacgcaaa     720

attgtggtca gcgttgccgc cgatattatg gcgctggtgc tgttaactgc gccgggtaaa     780

cagggcgcgg atattgtttt tggttcggcg caacgcttcg gcgtgccgat gggctacggt     840

ggcccacacg cggcattctt tgcggcgaaa gatgaataca aacgctcaat gccgggccgt     900

attatcggtg tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc gatgcagact     960

cgcgagcaac atatccgccg tgagaaagcg aactccaaca tttgtacttc ccaggtactg    1020

ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc cggttggcct gaaacgtatc    1080

gctaaccgca ttcaccgtct gaccgatatc ctggcggcgg gcctgcaaca aaaaggtctg    1140

aaactgcgcc atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga caaagcgggc    1200

gtactgacgc gtgccgaagc ggctgaaatc aacctgcgta gcgatattct gaacgcggtt    1260

gggatcaccc ttgatgaaac aaccacgcgt gaaaacgtaa tgcagctttt caacgtgctg    1320

ctgggcgata accacggcct ggacatcgac acgctggaca aagacgtggc tcacgacagc    1380

cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat    1440

cgctaccaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg    1500

gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaactgaa cgccgccgcc    1560

gagatgatcc caatcacctg gccggaattt gccgaactgc acccgttctg cccgccggag    1620

caggccgaag gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc    1680

ggttacgacg ccgtttgtat gcagccgaac tctggcgcac agggcgaata cgcgggcctg    1740

ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc    1800

ccggcttctg cgcacggaac taaccccgct tctgcacata tggcaggaat gcaggtggtg    1860

gttgtggcgt gtgataaaaa cggcaacatc gatctgactg atctgcgcgc gaaagcggaa    1920

caggcgggcg ataacctctc ctgtatcatg gtgacttatc cttctaccca cggcgtgtat    1980

gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt    2040

gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat tggtgcggac    2100

gtttcacacc ttaacctaca taaaactttc tgcattccgc acggcggtgg tggtccgggt    2160

atgggaccga tcggcgtgaa agcgcatttg gcaccgtttg taccgggtca tagcgtggtg    2220

caaatcgaag gcatgttaac ccgtcagggc gcggtttctg cggcaccgtt cggtagcgcc    2280

tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa    2340

gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc    2400

ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc    2460

ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct gatcgactac    2520

ggtttccacg cgccgacgat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact    2580

gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca    2640

gaaattgacc aggtgaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg    2700

ccgcacattc agagcgaact ggtcgccgag tgggcgcatc cgtacagccg tgaagttgcg    2760

gtattcccgg caggtgtggc agacaaatac tggccgacag tgaaacgtct ggatgatgtt    2820

tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaa          2874


<210> 13
<211> 454
<212> PRT
<213> Escherichia coli

<220> 
<223> L-serine dehydratase 1 (EC:4.3.1.17)

<400> 13
Met Ile Ser Leu Phe Asp Met Phe Lys Val Gly Ile Gly Pro Ser Ser 
1               5                   10                  15      
Ser His Thr Val Gly Pro Met Lys Ala Gly Lys Gln Phe Val Asp Asp 
            20                  25                  30          
Leu Val Glu Lys Gly Leu Leu Asp Ser Val Thr Arg Val Ala Val Asp 
        35                  40                  45              
Val Tyr Gly Ser Leu Ser Leu Thr Gly Lys Gly His His Thr Asp Ile 
    50                  55                  60                  
Ala Ile Ile Met Gly Leu Ala Gly Asn Glu Pro Ala Thr Val Asp Ile 
65                  70                  75                  80  
Asp Ser Ile Pro Gly Phe Ile Arg Asp Val Glu Glu Arg Glu Arg Leu 
                85                  90                  95      
Leu Leu Ala Gln Gly Arg His Glu Val Asp Phe Pro Arg Asp Asn Gly 
            100                 105                 110         
Met Arg Phe His Asn Gly Asn Leu Pro Leu His Glu Asn Gly Met Gln 
        115                 120                 125             
Ile His Ala Tyr Asn Gly Asp Glu Val Val Tyr Ser Lys Thr Tyr Tyr 
    130                 135                 140                 
Ser Ile Gly Gly Gly Phe Ile Val Asp Glu Glu His Phe Gly Gln Asp 
145                 150                 155                 160 
Ala Ala Asn Glu Val Ser Val Pro Tyr Pro Phe Lys Ser Ala Thr Glu 
                165                 170                 175     
Leu Leu Ala Tyr Cys Asn Glu Thr Gly Tyr Ser Leu Ser Gly Leu Ala 
            180                 185                 190         
Met Gln Asn Glu Leu Ala Leu His Ser Lys Lys Glu Ile Asp Glu Tyr 
        195                 200                 205             
Phe Ala His Val Trp Gln Thr Met Gln Ala Cys Ile Asp Arg Gly Met 
    210                 215                 220                 
Asn Thr Glu Gly Val Leu Pro Gly Pro Leu Arg Val Pro Arg Arg Ala 
225                 230                 235                 240 
Ser Ala Leu Arg Arg Met Leu Val Ser Ser Asp Lys Leu Ser Asn Asp 
                245                 250                 255     
Pro Met Asn Val Ile Asp Trp Val Asn Met Phe Ala Leu Ala Val Asn 
            260                 265                 270         
Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr Ala Pro Thr Asn Gly 
        275                 280                 285             
Ala Cys Gly Ile Val Pro Ala Val Leu Ala Tyr Tyr Asp His Phe Ile 
    290                 295                 300                 
Glu Ser Val Ser Pro Asp Ile Tyr Thr Arg Tyr Phe Met Ala Ala Gly 
305                 310                 315                 320 
Ala Ile Gly Ala Leu Tyr Lys Met Asn Ala Ser Ile Ser Gly Ala Glu 
                325                 330                 335     
Val Gly Cys Gln Gly Glu Val Gly Val Ala Cys Ser Met Ala Ala Ala 
            340                 345                 350         
Gly Leu Ala Glu Leu Leu Gly Gly Ser Pro Glu Gln Val Cys Val Ala 
        355                 360                 365             
Ala Glu Ile Gly Met Glu His Asn Leu Gly Leu Thr Cys Asp Pro Val 
    370                 375                 380                 
Ala Gly Gln Val Gln Val Pro Cys Ile Glu Arg Asn Ala Ile Ala Ser 
385                 390                 395                 400 
Val Lys Ala Ile Asn Ala Ala Arg Met Ala Leu Arg Arg Thr Ser Ala 
                405                 410                 415     
Pro Arg Val Ser Leu Asp Lys Val Ile Glu Thr Met Tyr Glu Thr Gly 
            420                 425                 430         
Lys Asp Met Asn Ala Lys Tyr Arg Glu Thr Ser Arg Gly Gly Leu Ala 
        435                 440                 445             
Ile Lys Val Gln Cys Asp 
    450                 

<210> 14
<211> 1365
<212> DNA
<213> Escherichia coli

<220> 
<223> L-serine dehydratase 1 (EC:4.3.1.17)

<400> 14
gtgattagtc tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta      60

gggcctatga aggcaggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat     120

agcgttactc gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac     180

cacaccgata tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc     240

gacagtattc ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag     300

ggacggcatg aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg     360

ccgctgcatg aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc     420

aaaacttatt attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat     480

gctgccaacg aagtaagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac     540

tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac     600

agcaagaaag agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc     660

gatcgcggga tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg     720

tctgccctgc gccggatgct ggtttccagc gataaactgt ctaacgatcc gatgaatgtc     780

attgactggg taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt     840

gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct ggcttactat     900

gaccacttta ttgaatcggt cagcccggac atctataccc gttactttat ggcagcgggc     960

gcgattggtg cattgtataa aatgaacgcc tctatttccg gtgcggaagt tggttgccag    1020

ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact gctgggcggt    1080

agcccggaac aggtttgcgt ggcggcggaa attggcatgg aacacaacct tggtttaacc    1140

tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct    1200

gtgaaggcga ttaacgccgc gcggatggct ctgcgccgca ccagtgcacc gcgcgtctcg    1260

ctggataagg tcatcgaaac gatgtacgaa accggtaagg acatgaacgc caaataccgc    1320

gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg actaa                    1365


<210> 15
<211> 417
<212> PRT
<213> Escherichia coli

<220> 
<223> serine hydroxymethyltransferase

<400> 15
Met Leu Lys Arg Glu Met Asn Ile Ala Asp Tyr Asp Ala Glu Leu Trp 
1               5                   10                  15      
Gln Ala Met Glu Gln Glu Lys Val Arg Gln Glu Glu His Ile Glu Leu 
            20                  25                  30          
Ile Ala Ser Glu Asn Tyr Thr Ser Pro Arg Val Met Gln Ala Gln Gly 
        35                  40                  45              
Ser Gln Leu Thr Asn Lys Tyr Ala Glu Gly Tyr Pro Gly Lys Arg Tyr 
    50                  55                  60                  
Tyr Gly Gly Cys Glu Tyr Val Asp Ile Val Glu Gln Leu Ala Ile Asp 
65                  70                  75                  80  
Arg Ala Lys Glu Leu Phe Gly Ala Asp Tyr Ala Asn Val Gln Pro His 
                85                  90                  95      
Ser Gly Ser Gln Ala Asn Phe Ala Val Tyr Thr Ala Leu Leu Glu Pro 
            100                 105                 110         
Gly Asp Thr Val Leu Gly Met Asn Leu Ala His Gly Gly His Leu Thr 
        115                 120                 125             
His Gly Ser Pro Val Asn Phe Ser Gly Lys Leu Tyr Asn Ile Val Pro 
    130                 135                 140                 
Tyr Gly Ile Asp Ala Thr Gly His Ile Asp Tyr Ala Asp Leu Glu Lys 
145                 150                 155                 160 
Gln Ala Lys Glu His Lys Pro Lys Met Ile Ile Gly Gly Phe Ser Ala 
                165                 170                 175     
Tyr Ser Gly Val Val Asp Trp Ala Lys Met Arg Glu Ile Ala Asp Ser 
            180                 185                 190         
Ile Gly Ala Tyr Leu Phe Val Asp Met Ala His Val Ala Gly Leu Val 
        195                 200                 205             
Ala Ala Gly Val Tyr Pro Asn Pro Val Pro His Ala His Val Val Thr 
    210                 215                 220                 
Thr Thr Thr His Lys Thr Leu Ala Gly Pro Arg Gly Gly Leu Ile Leu 
225                 230                 235                 240 
Ala Lys Gly Gly Ser Glu Glu Leu Tyr Lys Lys Leu Asn Ser Ala Val 
                245                 250                 255     
Phe Pro Gly Gly Gln Gly Gly Pro Leu Met His Val Ile Ala Gly Lys 
            260                 265                 270         
Ala Val Ala Leu Lys Glu Ala Met Glu Pro Glu Phe Lys Thr Tyr Gln 
        275                 280                 285             
Gln Gln Val Ala Lys Asn Ala Lys Ala Met Val Glu Val Phe Leu Glu 
    290                 295                 300                 
Arg Gly Tyr Lys Val Val Ser Gly Gly Thr Asp Asn His Leu Phe Leu 
305                 310                 315                 320 
Val Asp Leu Val Asp Lys Asn Leu Thr Gly Lys Glu Ala Asp Ala Ala 
                325                 330                 335     
Leu Gly Arg Ala Asn Ile Thr Val Asn Lys Asn Ser Val Pro Asn Asp 
            340                 345                 350         
Pro Lys Ser Pro Phe Val Thr Ser Gly Ile Arg Val Gly Thr Pro Ala 
        355                 360                 365             
Ile Thr Arg Arg Gly Phe Lys Glu Ala Glu Ala Lys Glu Leu Ala Gly 
    370                 375                 380                 
Trp Met Cys Asp Val Leu Asp Ser Ile Asn Asp Glu Ala Val Ile Glu 
385                 390                 395                 400 
Arg Ile Lys Gly Lys Val Leu Asp Ile Cys Ala Arg Tyr Pro Val Tyr 
                405                 410                 415     
Ala 
    

<210> 16
<211> 1254
<212> DNA
<213> Escherichia coli

<220> 
<223> serine hydroxymethyltransferase

<400> 16
atgttaaagc gtgaaatgaa cattgccgat tatgatgccg aactgtggca ggctatggag      60

caggaaaaag tacgtcagga agagcacatc gaactgatcg cctccgaaaa ctacaccagc     120

ccgcgcgtaa tgcaggcgca gggttctcag ctgaccaaca aatatgctga aggttatccg     180

ggcaaacgct actacggcgg ttgcgagtat gttgatatcg ttgaacaact ggcgatcgat     240

cgtgcgaaag aactgttcgg cgctgactac gctaacgtcc agccgcactc cggctcccag     300

gctaactttg cggtctacac cgcgctgctg gaaccaggtg ataccgttct gggtatgaac     360

ctggcgcatg gcggtcacct gactcacggt tctccggtta acttctccgg taaactgtac     420

aacatcgttc cttacggtat cgatgctacc ggtcatatcg actacgccga tctggaaaaa     480

caagccaaag aacacaagcc gaaaatgatt atcggtggtt tctctgcata ttccggcgtg     540

gtggactggg cgaaaatgcg tgaaatcgct gacagcatcg gtgcttacct gttcgttgat     600

atggcgcacg ttgcgggcct ggttgctgct ggcgtctacc cgaacccggt tcctcatgct     660

cacgttgtta ctaccaccac tcacaaaacc ctggcgggtc cgcgcggcgg cctgatcctg     720

gcgaaaggtg gtagcgaaga gctgtacaaa aaactgaact ctgccgtttt ccctggtggt     780

cagggcggtc cgttgatgca cgtaatcgcc ggtaaagcgg ttgctctgaa agaagcgatg     840

gagcctgagt tcaaaactta ccagcagcag gtcgctaaaa acgctaaagc gatggtagaa     900

gtgttcctcg agcgcggcta caaagtggtt tccggcggca ctgataacca cctgttcctg     960

gttgatctgg ttgataaaaa cctgaccggt aaagaagcag acgccgctct gggccgtgct    1020

aacatcaccg tcaacaaaaa cagcgtaccg aacgatccga agagcccgtt tgtgacctcc    1080

ggtattcgtg taggtactcc ggcgattacc cgtcgcggct ttaaagaagc cgaagcgaaa    1140

gaactggctg gctggatgtg tgacgtgctg gacagcatca atgatgaagc cgttatcgag    1200

cgcatcaaag gtaaagttct cgacatctgc gcacgttacc cggtttacgc ataa          1254


<210> 17
<211> 401
<212> PRT
<213> Pseudomonas sp.

<220> 
<223> Formate dehydrogenase (EC:1.17.1.9)

<400> 17
Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 
1               5                   10                  15      
Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 
            20                  25                  30          
Gly Gly Gln Thr Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 
        35                  40                  45              
Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Lys Tyr Leu 
    50                  55                  60                  
Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 
65                  70                  75                  80  
Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 
                85                  90                  95      
Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 
            100                 105                 110         
Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 
        115                 120                 125             
Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 
    130                 135                 140                 
Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 
145                 150                 155                 160 
Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 
                165                 170                 175     
Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 
            180                 185                 190         
Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 
        195                 200                 205             
Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Asp Arg His 
    210                 215                 220                 
Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 
225                 230                 235                 240 
Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 
                245                 250                 255     
Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 
            260                 265                 270         
Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 
        275                 280                 285             
Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 
    290                 295                 300                 
Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 
305                 310                 315                 320 
Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 
                325                 330                 335     
Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 
            340                 345                 350         
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 
        355                 360                 365             
Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 
    370                 375                 380                 
Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 
385                 390                 395                 400 
Val 
    

<210> 18
<211> 1206
<212> DNA
<213> Pseudomonas sp.

<220> 
<223> Formate dehydrogenase (EC:1.17.1.9)

<400> 18
atggctaaag ttctgtgcgt tctgtacgac gacccggttg acggttaccc gaaaacctac      60

gctcgtgacg acctgccgaa aatcgaccac tacccgggtg gtcagaccct gccgaccccg     120

aaagctatcg acttcacccc gggtcagctg ctgggttctg tttctggtga actgggtctg     180

cgtaaatacc tggaatctaa cggtcacacc ctggttgtta cctctgacaa agacggtccg     240

gactctgttt tcgaacgtga actggttgac gctgacgttg ttatctctca gccgttctgg     300

ccggcttacc tgaccccgga acgtatcgct aaagctaaaa acctgaaact ggctctgacc     360

gctggtatcg gttctgacca cgttgacctg caatctgcta tcgaccgtaa cgttaccgtt     420

gctgaagtta cctactgcaa ctctatctct gttgctgaac acgttgttat gatgatcctg     480

tctctggttc gtaactacct gccgtctcac gaatgggctc gtaaaggtgg ttggaacata     540

gctgactgcg taagccacgc ttacgacctg gaagctatgc acgttggtac cgttgctgct     600

ggtcgtatcg gtctggctgt tctgcgtcgt ctggctccgt tcgacgttca cctgcactac     660

accgaccgtc accgtctgcc ggaatctgtt gaaaaagaac tgaacctgac ctggcacgct     720

acccgtgaag acatgtaccc ggtttgcgac gttgttaccc tgaactgccc gctgcacccg     780

gaaaccgaac acatgatcaa cgacgaaacc ctgaaactgt tcaaacgtgg tgcttacatc     840

gttaacaccg ctcgtggtaa actgtgcgac cgtgacgctg ttgctcgtgc tctggaatct     900

ggtcgtctgg ctggttatgc gggtgacgtg tggttccccc agccggctcc gaaagaccac     960

ccgtggcgta ccatgccgta caacggtatg accccgcaca tctctggtac caccctgacc    1020

gctcaggctc gttacgctgc tggtacccgt gaaatcctgg aatgcttctt cgaaggtcgt    1080

ccgatccgtg acgaatacct gatcgttcag ggtggtgctc tggctggtac cggtgctcac    1140

tcttactcta aaggtaacgc taccggtggt tctgaagaag ctgctaaatt caaaaaagct    1200

gtttaa                                                               1206


<210> 19
<211> 510
<212> PRT
<213> Escherichia coli

<220> 
<223> NAD(P) transhydrogenase subunit alpha (EC:7.1.1.1)

<400> 19
Met Arg Ile Gly Ile Pro Arg Glu Arg Leu Thr Asn Glu Thr Arg Val 
1               5                   10                  15      
Ala Ala Thr Pro Lys Thr Val Glu Gln Leu Leu Lys Leu Gly Phe Thr 
            20                  25                  30          
Val Ala Val Glu Ser Gly Ala Gly Gln Leu Ala Ser Phe Asp Asp Lys 
        35                  40                  45              
Ala Phe Val Gln Ala Gly Ala Glu Ile Val Glu Gly Asn Ser Val Trp 
    50                  55                  60                  
Gln Ser Glu Ile Ile Leu Lys Val Asn Ala Pro Leu Asp Asp Glu Ile 
65                  70                  75                  80  
Ala Leu Leu Asn Pro Gly Thr Thr Leu Val Ser Phe Ile Trp Pro Ala 
                85                  90                  95      
Gln Asn Pro Glu Leu Met Gln Lys Leu Ala Glu Arg Asn Val Thr Val 
            100                 105                 110         
Met Ala Met Asp Ser Val Pro Arg Ile Ser Arg Ala Gln Ser Leu Asp 
        115                 120                 125             
Ala Leu Ser Ser Met Ala Asn Ile Ala Gly Tyr Arg Ala Ile Val Glu 
    130                 135                 140                 
Ala Ala His Glu Phe Gly Arg Phe Phe Thr Gly Gln Ile Thr Ala Ala 
145                 150                 155                 160 
Gly Lys Val Pro Pro Ala Lys Val Met Val Ile Gly Ala Gly Val Ala 
                165                 170                 175     
Gly Leu Ala Ala Ile Gly Ala Ala Asn Ser Leu Gly Ala Ile Val Arg 
            180                 185                 190         
Ala Phe Asp Thr Arg Pro Glu Val Lys Glu Gln Val Gln Ser Met Gly 
        195                 200                 205             
Ala Glu Phe Leu Glu Leu Asp Phe Lys Glu Glu Ala Gly Ser Gly Asp 
    210                 215                 220                 
Gly Tyr Ala Lys Val Met Ser Asp Ala Phe Ile Lys Ala Glu Met Glu 
225                 230                 235                 240 
Leu Phe Ala Ala Gln Ala Lys Glu Val Asp Ile Ile Val Thr Thr Ala 
                245                 250                 255     
Leu Ile Pro Gly Lys Pro Ala Pro Lys Leu Ile Thr Arg Glu Met Val 
            260                 265                 270         
Asp Ser Met Lys Ala Gly Ser Val Ile Val Asp Leu Ala Ala Gln Asn 
        275                 280                 285             
Gly Gly Asn Cys Glu Tyr Thr Val Pro Gly Glu Ile Phe Thr Thr Glu 
    290                 295                 300                 
Asn Gly Val Lys Val Ile Gly Tyr Thr Asp Leu Pro Gly Arg Leu Pro 
305                 310                 315                 320 
Thr Gln Ser Ser Gln Leu Tyr Gly Thr Asn Leu Val Asn Leu Leu Lys 
                325                 330                 335     
Leu Leu Cys Lys Glu Lys Asp Gly Asn Ile Thr Val Asp Phe Asp Asp 
            340                 345                 350         
Val Val Ile Arg Gly Val Thr Val Ile Arg Ala Gly Glu Ile Thr Trp 
        355                 360                 365             
Pro Ala Pro Pro Ile Gln Val Ser Ala Gln Pro Gln Ala Ala Gln Lys 
    370                 375                 380                 
Ala Ala Pro Glu Val Lys Thr Glu Glu Lys Cys Thr Cys Ser Pro Trp 
385                 390                 395                 400 
Arg Lys Tyr Ala Leu Met Ala Leu Ala Ile Ile Leu Phe Gly Trp Met 
                405                 410                 415     
Ala Ser Val Ala Pro Lys Glu Phe Leu Gly His Phe Thr Val Phe Ala 
            420                 425                 430         
Leu Ala Cys Val Val Gly Tyr Tyr Val Val Trp Asn Val Ser His Ala 
        435                 440                 445             
Leu His Thr Pro Leu Met Ser Val Thr Asn Ala Ile Ser Gly Ile Ile 
    450                 455                 460                 
Val Val Gly Ala Leu Leu Gln Ile Gly Gln Gly Gly Trp Val Ser Phe 
465                 470                 475                 480 
Leu Ser Phe Ile Ala Val Leu Ile Ala Ser Ile Asn Ile Phe Gly Gly 
                485                 490                 495     
Phe Thr Val Thr Gln Arg Met Leu Lys Met Phe Arg Lys Asn 
            500                 505                 510 

<210> 20
<211> 1533
<212> DNA
<213> Escherichia coli

<220> 
<223> NAD(P) transhydrogenase subunit alpha (EC:7.1.1.1)

<400> 20
atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca      60

aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt     120

caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg     180

aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt     240

gcgttactga atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa     300

ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt     360

atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaacatcgc cggttatcgc     420

gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat tactgcggcc     480

gggaaagtgc caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc     540

attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg     600

aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagct     660

ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa     720

ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc     780

aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg     840

attgtcgacc tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc     900

ttcactacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg     960

acgcaatcct cacagcttta cggcacaaac ctcgttaatc tgctgaaact gttgtgcaaa    1020

gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg    1080

atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag    1140

gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtacctg ctcaccgtgg    1200

cgtaaatacg cgttgatggc gctggcaatc attctttttg gctggatggc aagcgttgcg    1260

ccgaaagaat tccttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac    1320

gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggtcac caacgcgatt    1380

tcagggatta ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc    1440

cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact    1500

cagcgcatgc tgaaaatgtt ccgcaaaaat taa                                 1533


<210> 21
<211> 462
<212> PRT
<213> Escherichia coli

<220> 
<223> NAD(P) transhydrogenase subunit beta (EC:7.1.1.1)

<400> 21
Met Ser Gly Gly Leu Val Thr Ala Ala Tyr Ile Val Ala Ala Ile Leu 
1               5                   10                  15      
Phe Ile Phe Ser Leu Ala Gly Leu Ser Lys His Glu Thr Ser Arg Gln 
            20                  25                  30          
Gly Asn Asn Phe Gly Ile Ala Gly Met Ala Ile Ala Leu Ile Ala Thr 
        35                  40                  45              
Ile Phe Gly Pro Asp Thr Gly Asn Val Gly Trp Ile Leu Leu Ala Met 
    50                  55                  60                  
Val Ile Gly Gly Ala Ile Gly Ile Arg Leu Ala Lys Lys Val Glu Met 
65                  70                  75                  80  
Thr Glu Met Pro Glu Leu Val Ala Ile Leu His Ser Phe Val Gly Leu 
                85                  90                  95      
Ala Ala Val Leu Val Gly Phe Asn Ser Tyr Leu His His Asp Ala Gly 
            100                 105                 110         
Met Ala Pro Ile Leu Val Asn Ile His Leu Thr Glu Val Phe Leu Gly 
        115                 120                 125             
Ile Phe Ile Gly Ala Val Thr Phe Thr Gly Ser Val Val Ala Phe Gly 
    130                 135                 140                 
Lys Leu Cys Gly Lys Ile Ser Ser Lys Pro Leu Met Leu Pro Asn Arg 
145                 150                 155                 160 
His Lys Met Asn Leu Ala Ala Leu Val Val Ser Phe Leu Leu Leu Ile 
                165                 170                 175     
Val Phe Val Arg Thr Asp Ser Val Gly Leu Gln Val Leu Ala Leu Leu 
            180                 185                 190         
Ile Met Thr Ala Ile Ala Leu Val Phe Gly Trp His Leu Val Ala Ser 
        195                 200                 205             
Ile Gly Gly Ala Asp Met Pro Val Val Val Ser Met Leu Asn Ser Tyr 
    210                 215                 220                 
Ser Gly Trp Ala Ala Ala Ala Ala Gly Phe Met Leu Ser Asn Asp Leu 
225                 230                 235                 240 
Leu Ile Val Thr Gly Ala Leu Val Gly Ser Ser Gly Ala Ile Leu Ser 
                245                 250                 255     
Tyr Ile Met Cys Lys Ala Met Asn Arg Ser Phe Ile Ser Val Ile Ala 
            260                 265                 270         
Gly Gly Phe Gly Thr Asp Gly Ser Ser Thr Gly Asp Asp Gln Glu Val 
        275                 280                 285             
Gly Glu His Arg Glu Ile Thr Ala Glu Glu Thr Ala Glu Leu Leu Lys 
    290                 295                 300                 
Asn Ser His Ser Val Ile Ile Thr Pro Gly Tyr Gly Met Ala Val Ala 
305                 310                 315                 320 
Gln Ala Gln Tyr Pro Val Ala Glu Ile Thr Glu Lys Leu Arg Ala Arg 
                325                 330                 335     
Gly Ile Asn Val Arg Phe Gly Ile His Pro Val Ala Gly Arg Leu Pro 
            340                 345                 350         
Gly His Met Asn Val Leu Leu Ala Glu Ala Lys Val Pro Tyr Asp Ile 
        355                 360                 365             
Val Leu Glu Met Asp Glu Ile Asn Asp Asp Phe Ala Asp Thr Asp Thr 
    370                 375                 380                 
Val Leu Val Ile Gly Ala Asn Asp Thr Val Asn Pro Ala Ala Gln Asp 
385                 390                 395                 400 
Asp Pro Lys Ser Pro Ile Ala Gly Met Pro Val Leu Glu Val Trp Lys 
                405                 410                 415     
Ala Gln Asn Val Ile Val Phe Lys Arg Ser Met Asn Thr Gly Tyr Ala 
            420                 425                 430         
Gly Val Gln Asn Pro Leu Phe Phe Lys Glu Asn Thr His Met Leu Phe 
        435                 440                 445             
Gly Asp Ala Lys Ala Ser Val Asp Ala Ile Leu Lys Ala Leu 
    450                 455                 460         

<210> 22
<211> 1389
<212> DNA
<213> Escherichia coli

<220> 
<223> NAD(P) transhydrogenase subunit beta (EC:7.1.1.1)

<400> 22
atgtctggag gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt      60

ctggccggtc tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg     120

atggcgattg cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc     180

ttgctggcga tggtcattgg tggggcaatt ggtatccgtc tggcgaagaa agttgaaatg     240

accgaaatgc cagaactggt ggcgatcctg catagcttcg tgggtctggc ggcagtgctg     300

gttggcttta acagctatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt     360

cacctgacgg aagtgttcct cggtatcttc atcggggcgg taacgttcac gggttcggtg     420

gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac cattgatgct gccaaaccgt     480

cacaaaatga acctggcggc tctggtcgtt tccttcctgc tgctgattgt atttgttcgc     540

acggacagcg tcggcctgca agtgctggca ttgctgataa tgaccgcaat tgcgctggta     600

ttcggctggc atttagtcgc ctccatcggt ggtgcagata tgccagtggt ggtgtcgatg     660

ctgaactcgt actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg     720

ctgattgtga ccggtgcgct ggtcggttct tcgggggcta tcctttctta cattatgtgt     780

aaggcgatga accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct     840

tctactggcg atgatcagga agtgggtgag caccgcgaaa tcaccgcaga agagacagcg     900

gaactgctga aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg     960

caggcgcaat atcctgtcgc tgaaattact gagaaattgc gcgctcgtgg tattaatgtg    1020

cgtttcggta tccacccggt cgcggggcgt ttgcctggac atatgaacgt attgctggct    1080

gaagcaaaag taccgtatga catcgtgctg gaaatggacg agatcaatga tgactttgct    1140

gataccgata ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat    1200

gatccgaaga gtccgattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg    1260

attgtcttta aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc    1320

aaggaaaaca cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa    1380

gctctgtaa                                                            1389


<210> 23
<211> 468
<212> PRT
<213> Escherichia coli

<220> 
<223> 6-phosphogluconate dehydrogenase (Gnd, EC 1.1.1.44)

<400> 23
Met Ser Lys Gln Gln Ile Gly Val Val Gly Met Ala Val Met Gly Arg 
1               5                   10                  15      
Asn Leu Ala Leu Asn Ile Glu Ser Arg Gly Tyr Thr Val Ser Ile Phe 
            20                  25                  30          
Asn Arg Ser Arg Glu Lys Thr Glu Glu Val Ile Ala Glu Asn Pro Gly 
        35                  40                  45              
Lys Lys Leu Val Pro Tyr Tyr Thr Val Lys Glu Phe Val Glu Ser Leu 
    50                  55                  60                  
Glu Thr Pro Arg Arg Ile Leu Leu Met Val Lys Ala Gly Ala Gly Thr 
65                  70                  75                  80  
Asp Ala Ala Ile Asp Ser Leu Lys Pro Tyr Leu Asp Lys Gly Asp Ile 
                85                  90                  95      
Ile Ile Asp Gly Gly Asn Thr Phe Phe Gln Asp Thr Ile Arg Arg Asn 
            100                 105                 110         
Arg Glu Leu Ser Ala Glu Gly Phe Asn Phe Ile Gly Thr Gly Val Ser 
        115                 120                 125             
Gly Gly Glu Glu Gly Ala Leu Lys Gly Pro Ser Ile Met Pro Gly Gly 
    130                 135                 140                 
Gln Lys Glu Ala Tyr Glu Leu Val Ala Pro Ile Leu Thr Lys Ile Ala 
145                 150                 155                 160 
Ala Val Ala Glu Asp Gly Glu Pro Cys Val Thr Tyr Ile Gly Ala Asp 
                165                 170                 175     
Gly Ala Gly His Tyr Val Lys Met Val His Asn Gly Ile Glu Tyr Gly 
            180                 185                 190         
Asp Met Gln Leu Ile Ala Glu Ala Tyr Ser Leu Leu Lys Gly Gly Leu 
        195                 200                 205             
Asn Leu Thr Asn Glu Glu Leu Ala Gln Thr Phe Thr Glu Trp Asn Asn 
    210                 215                 220                 
Gly Glu Leu Ser Ser Tyr Leu Ile Asp Ile Thr Lys Asp Ile Phe Thr 
225                 230                 235                 240 
Lys Lys Asp Glu Asp Gly Asn Tyr Leu Val Asp Val Ile Leu Asp Glu 
                245                 250                 255     
Ala Ala Asn Lys Gly Thr Gly Lys Trp Thr Ser Gln Ser Ala Leu Asp 
            260                 265                 270         
Leu Gly Glu Pro Leu Ser Leu Ile Thr Glu Ser Val Phe Ala Arg Tyr 
        275                 280                 285             
Ile Ser Ser Leu Lys Asp Gln Arg Val Ala Ala Ser Lys Val Leu Ser 
    290                 295                 300                 
Gly Pro Gln Ala Gln Pro Ala Gly Asp Lys Ala Glu Phe Ile Glu Lys 
305                 310                 315                 320 
Val Arg Arg Ala Leu Tyr Leu Gly Lys Ile Val Ser Tyr Ala Gln Gly 
                325                 330                 335     
Phe Ser Gln Leu Arg Ala Ala Ser Glu Glu Tyr Asn Trp Asp Leu Asn 
            340                 345                 350         
Tyr Gly Glu Ile Ala Lys Ile Phe Arg Ala Gly Cys Ile Ile Arg Ala 
        355                 360                 365             
Gln Phe Leu Gln Lys Ile Thr Asp Ala Tyr Ala Glu Asn Pro Gln Ile 
    370                 375                 380                 
Ala Asn Leu Leu Leu Ala Pro Tyr Phe Lys Gln Ile Ala Asp Asp Tyr 
385                 390                 395                 400 
Gln Gln Ala Leu Arg Asp Val Val Ala Tyr Ala Val Gln Asn Gly Ile 
                405                 410                 415     
Pro Val Pro Thr Phe Ser Ala Ala Val Ala Tyr Tyr Asp Ser Tyr Arg 
            420                 425                 430         
Ala Ala Val Leu Pro Ala Asn Leu Ile Gln Ala Gln Arg Asp Tyr Phe 
        435                 440                 445             
Gly Ala His Thr Tyr Lys Arg Ile Asp Lys Glu Gly Val Phe His Thr 
    450                 455                 460                 
Glu Trp Leu Asp 
465             

<210> 24
<211> 1407
<212> DNA
<213> Escherichia coli

<220> 
<223> 6-phosphogluconate dehydrogenase (Gnd, EC 1.1.1.44)

<400> 24
atgtccaagc aacagatcgg cgtagtcggt atggcagtga tgggacgcaa ccttgcgctc      60

aacatcgaaa gccgtggtta taccgtctct attttcaacc gttcccgtga gaagacggaa     120

gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt actatacggt gaaagagttt     180

gtcgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg     240

gatgctgcta ttgattccct caaaccatat ctcgataaag gagacatcat cattgatggt     300

ggtaacacct tcttccagga cactattcgt cgtaatcgtg agctttcagc agagggcttt     360

aacttcatcg gtaccggtgt ttctggcggt gaagaggggg cgctgaaagg tccttctatt     420

atgcctggtg gccagaaaga agcctatgaa ttggtagcac cgatcctgac caaaatcgcc     480

gccgtagctg aagacggtga accatgcgtt acctatattg gtgccgatgg cgcaggtcac     540

tatgtgaaga tggttcacaa cggtattgaa tacggcgata tgcagctgat tgctgaagcc     600

tattctctgc ttaaaggtgg cctgaacctc accaacgaag aactggcgca gacctttacc     660

gagtggaata acggtgaact gagcagttac ctgatcgaca tcaccaaaga tatcttcacc     720

aaaaaagatg aagacggtaa ctacctggtt gatgtgatcc tggatgaagc ggctaacaaa     780

ggtaccggta aatggaccag ccagagcgcg ctggatctcg gcgaaccgct gtcgctgatt     840

accgagtctg tgtttgcacg ttatatctct tctctgaaag atcagcgtgt tgccgcatct     900

aaagttctct ctggtccgca agcacagcca gcaggcgaca aggctgagtt catcgaaaaa     960

gttcgtcgtg cgctgtatct gggcaaaatc gtttcttacg cccagggctt ctctcagctg    1020

cgtgctgcgt ctgaagagta caactgggat ctgaactacg gcgaaatcgc gaagattttc    1080

cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc ttatgccgaa    1140

aatccacaga tcgctaacct gttgctggct ccgtacttca agcaaattgc cgatgactac    1200

cagcaggcgc tgcgtgatgt cgttgcttat gcagtacaga acggtattcc ggttccgacc    1260

ttctccgcag cggttgccta ttacgacagc taccgtgctg ctgttctgcc tgcgaacctg    1320

atccaggcac agcgtgacta ttttggtgcg catacttata agcgtattga taaagaaggt    1380

gtgttccata ccgaatggct ggattaa                                        1407


<210> 25
<211> 416
<212> PRT
<213> Escherichia coli

<220> 
<223> isocitrate dehydrogenase (Icd, 1.1.1.41-42)

<400> 25
Met Glu Ser Lys Val Val Val Pro Ala Gln Gly Lys Lys Ile Thr Leu 
1               5                   10                  15      
Gln Asn Gly Lys Leu Asn Val Pro Glu Asn Pro Ile Ile Pro Tyr Ile 
            20                  25                  30          
Glu Gly Asp Gly Ile Gly Val Asp Val Thr Pro Ala Met Leu Lys Val 
        35                  40                  45              
Val Asp Ala Ala Val Glu Lys Ala Tyr Lys Gly Glu Arg Lys Ile Ser 
    50                  55                  60                  
Trp Met Glu Ile Tyr Thr Gly Glu Lys Ser Thr Gln Val Tyr Gly Gln 
65                  70                  75                  80  
Asp Val Trp Leu Pro Ala Glu Thr Leu Asp Leu Ile Arg Glu Tyr Arg 
                85                  90                  95      
Val Ala Ile Lys Gly Pro Leu Thr Thr Pro Val Gly Gly Gly Ile Arg 
            100                 105                 110         
Ser Leu Asn Val Ala Leu Arg Gln Glu Leu Asp Leu Tyr Ile Cys Leu 
        115                 120                 125             
Arg Pro Val Arg Tyr Tyr Gln Gly Thr Pro Ser Pro Val Lys His Pro 
    130                 135                 140                 
Glu Leu Thr Asp Met Val Ile Phe Arg Glu Asn Ser Glu Asp Ile Tyr 
145                 150                 155                 160 
Ala Gly Ile Glu Trp Lys Ala Asp Ser Ala Asp Ala Glu Lys Val Ile 
                165                 170                 175     
Lys Phe Leu Arg Glu Glu Met Gly Val Lys Lys Ile Arg Phe Pro Glu 
            180                 185                 190         
His Cys Gly Ile Gly Ile Lys Pro Cys Ser Glu Glu Gly Thr Lys Arg 
        195                 200                 205             
Leu Val Arg Ala Ala Ile Glu Tyr Ala Ile Ala Asn Asp Arg Asp Ser 
    210                 215                 220                 
Val Thr Leu Val His Lys Gly Asn Ile Met Lys Phe Thr Glu Gly Ala 
225                 230                 235                 240 
Phe Lys Asp Trp Gly Tyr Gln Leu Ala Arg Glu Glu Phe Gly Gly Glu 
                245                 250                 255     
Leu Ile Asp Gly Gly Pro Trp Leu Lys Val Lys Asn Pro Asn Thr Gly 
            260                 265                 270         
Lys Glu Ile Val Ile Lys Asp Val Ile Ala Asp Ala Phe Leu Gln Gln 
        275                 280                 285             
Ile Leu Leu Arg Pro Ala Glu Tyr Asp Val Ile Ala Cys Met Asn Leu 
    290                 295                 300                 
Asn Gly Asp Tyr Ile Ser Asp Ala Leu Ala Ala Gln Val Gly Gly Ile 
305                 310                 315                 320 
Gly Ile Ala Pro Gly Ala Asn Ile Gly Asp Glu Cys Ala Leu Phe Glu 
                325                 330                 335     
Ala Thr His Gly Thr Ala Pro Lys Tyr Ala Gly Gln Asp Lys Val Asn 
            340                 345                 350         
Pro Gly Ser Ile Ile Leu Ser Ala Glu Met Met Leu Arg His Met Gly 
        355                 360                 365             
Trp Thr Glu Ala Ala Asp Leu Ile Val Lys Gly Met Glu Gly Ala Ile 
    370                 375                 380                 
Asn Ala Lys Thr Val Thr Tyr Asp Phe Glu Arg Leu Met Asp Gly Ala 
385                 390                 395                 400 
Lys Leu Leu Lys Cys Ser Glu Phe Gly Asp Ala Ile Ile Glu Asn Met 
                405                 410                 415     



<210> 26
<211> 1251
<212> DNA
<213> Escherichia coli

<220> 
<223> isocitrate dehydrogenase (Icd, 1.1.1.41-42)

<400> 26
atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa      60

ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat     120

gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag     180

cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag     240

gacgtctggc tgcctgctga aactcttgat ctgattcgtg aatatcgcgt tgccattaaa     300

ggtccgctga ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag     360

gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg     420

gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat     480

gcgggtatcg aatggaaagc agactctgcc gacgccgaga aagtgattaa attcctgcgt     540

gaagagatgg gggtgaagaa aattcgcttc ccggaacatt gtggtatcgg tattaagccg     600

tgttcggaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac     660

gatcgtgact ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggagcg     720

tttaaagact ggggctacca gctggcgcgt gaagagtttg gcggtgaact gatcgacggt     780

ggcccgtggc tgaaagttaa aaacccgaac actggcaaag agatcgtcat taaagacgtg     840

attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc     900

tgtatgaacc tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc     960

ggtatcgccc ctggtgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt    1020

actgcgccga aatatgccgg tcaggacaaa gtaaatcctg gctctattat tctctccgct    1080

gagatgatgc tgcgccacat gggttggacc gaagcggctg acttaattgt taaaggtatg    1140

gaaggcgcaa tcaacgcgaa aaccgtaacc tatgacttcg agcgtctgat ggatggcgct    1200

aaactgctga aatgttcaga gtttggtgac gcgatcatcg aaaacatgta a             1251


<210> 27
<211> 759
<212> PRT
<213> Escherichia coli

<220> 
<223> malic enzyme (MaeB, EC 1.1.1.38-40)

<400> 27
Met Asp Asp Gln Leu Lys Gln Ser Ala Leu Asp Phe His Glu Phe Pro 
1               5                   10                  15      
Val Pro Gly Lys Ile Gln Val Ser Pro Thr Lys Pro Leu Ala Thr Gln 
            20                  25                  30          
Arg Asp Leu Ala Leu Ala Tyr Ser Pro Gly Val Ala Ala Pro Cys Leu 
        35                  40                  45              
Glu Ile Glu Lys Asp Pro Leu Lys Ala Tyr Lys Tyr Thr Ala Arg Gly 
    50                  55                  60                  
Asn Leu Val Ala Val Ile Ser Asn Gly Thr Ala Val Leu Gly Leu Gly 
65                  70                  75                  80  
Asn Ile Gly Ala Leu Ala Gly Lys Pro Val Met Glu Gly Lys Gly Val 
                85                  90                  95      
Leu Phe Lys Lys Phe Ala Gly Ile Asp Val Phe Asp Ile Glu Val Asp 
            100                 105                 110         
Glu Leu Asp Pro Asp Lys Phe Ile Glu Val Val Ala Ala Leu Glu Pro 
        115                 120                 125             
Thr Phe Gly Gly Ile Asn Leu Glu Asp Ile Lys Ala Pro Glu Cys Phe 
    130                 135                 140                 
Tyr Ile Glu Gln Lys Leu Arg Glu Arg Met Asn Ile Pro Val Phe His 
145                 150                 155                 160 
Asp Asp Gln His Gly Thr Ala Ile Ile Ser Thr Ala Ala Ile Leu Asn 
                165                 170                 175     
Gly Leu Arg Val Val Glu Lys Asn Ile Ser Asp Val Arg Met Val Val 
            180                 185                 190         
Ser Gly Ala Gly Ala Ala Ala Ile Ala Cys Met Asn Leu Leu Val Ala 
        195                 200                 205             
Leu Gly Leu Gln Lys His Asn Ile Val Val Cys Asp Ser Lys Gly Val 
    210                 215                 220                 
Ile Tyr Gln Gly Arg Glu Pro Asn Met Ala Glu Thr Lys Ala Ala Tyr 
225                 230                 235                 240 
Ala Val Val Asp Asp Gly Lys Arg Thr Leu Asp Asp Val Ile Glu Gly 
                245                 250                 255     
Ala Asp Ile Phe Leu Gly Cys Ser Gly Pro Lys Val Leu Thr Gln Glu 
            260                 265                 270         
Met Val Lys Lys Met Ala Arg Ala Pro Met Ile Leu Ala Leu Ala Asn 
        275                 280                 285             
Pro Glu Pro Glu Ile Leu Pro Pro Leu Ala Lys Glu Val Arg Pro Asp 
    290                 295                 300                 
Ala Ile Ile Cys Thr Gly Arg Ser Asp Tyr Pro Asn Gln Val Asn Asn 
305                 310                 315                 320 
Val Leu Cys Phe Pro Phe Ile Phe Arg Gly Ala Leu Asp Val Gly Ala 
                325                 330                 335     
Thr Ala Ile Asn Glu Glu Met Lys Leu Ala Ala Val Arg Ala Ile Ala 
            340                 345                 350         
Glu Leu Ala His Ala Glu Gln Ser Glu Val Val Ala Ser Ala Tyr Gly 
        355                 360                 365             
Asp Gln Asp Leu Ser Phe Gly Pro Glu Tyr Ile Ile Pro Lys Pro Phe 
    370                 375                 380                 
Asp Pro Arg Leu Ile Val Lys Ile Ala Pro Ala Val Ala Lys Ala Ala 
385                 390                 395                 400 
Met Glu Ser Gly Val Ala Thr Arg Pro Ile Ala Asp Phe Asp Val Tyr 
                405                 410                 415     
Ile Asp Lys Leu Thr Glu Phe Val Tyr Lys Thr Asn Leu Phe Met Lys 
            420                 425                 430         
Pro Ile Phe Ser Gln Ala Arg Lys Ala Pro Lys Arg Val Val Leu Pro 
        435                 440                 445             
Glu Gly Glu Glu Ala Arg Val Leu His Ala Thr Gln Glu Leu Val Thr 
    450                 455                 460                 
Leu Gly Leu Ala Lys Pro Ile Leu Ile Gly Arg Pro Asn Val Ile Glu 
465                 470                 475                 480 
Met Arg Ile Gln Lys Leu Gly Leu Gln Ile Lys Ala Gly Val Asp Phe 
                485                 490                 495     
Glu Ile Val Asn Asn Glu Ser Asp Pro Arg Phe Lys Glu Tyr Trp Thr 
            500                 505                 510         
Glu Tyr Phe Gln Ile Met Lys Arg Arg Gly Val Thr Gln Glu Gln Ala 
        515                 520                 525             
Gln Arg Ala Leu Ile Ser Asn Pro Thr Val Ile Gly Ala Ile Met Val 
    530                 535                 540                 
Gln Arg Gly Glu Ala Asp Ala Met Ile Cys Gly Thr Val Gly Asp Tyr 
545                 550                 555                 560 
His Glu His Phe Ser Val Val Lys Asn Val Phe Gly Tyr Arg Asp Gly 
                565                 570                 575     
Val His Thr Ala Gly Ala Met Asn Ala Leu Leu Leu Pro Ser Gly Asn 
            580                 585                 590         
Thr Phe Ile Ala Asp Thr Tyr Val Asn Asp Glu Pro Asp Ala Glu Glu 
        595                 600                 605             
Leu Ala Glu Ile Thr Leu Met Ala Ala Glu Thr Val Arg Arg Phe Gly 
    610                 615                 620                 
Ile Glu Pro Arg Val Ala Leu Leu Ser His Ser Asn Phe Gly Ser Ser 
625                 630                 635                 640 
Asp Cys Pro Ser Ser Ser Lys Met Arg Gln Ala Leu Glu Leu Val Arg 
                645                 650                 655     
Glu Arg Ala Pro Glu Leu Met Ile Asp Gly Glu Met His Gly Asp Ala 
            660                 665                 670         
Ala Leu Val Glu Ala Ile Arg Asn Asp Arg Met Pro Asp Ser Ser Leu 
        675                 680                 685             
Lys Gly Ser Ala Asn Ile Leu Val Met Pro Asn Met Glu Ala Ala Arg 
    690                 695                 700                 
Ile Ser Tyr Asn Leu Leu Arg Val Ser Ser Ser Glu Gly Val Thr Val 
705                 710                 715                 720 
Gly Pro Val Leu Met Gly Val Ala Lys Pro Val His Val Leu Thr Pro 
                725                 730                 735     
Ile Ala Ser Val Arg Arg Ile Val Asn Met Val Ala Leu Ala Val Val 
            740                 745                 750         
Glu Ala Gln Thr Gln Pro Leu 
        755                 

<210> 28
<211> 2280
<212> DNA
<213> Escherichia coli

<220> 
<223> malic enzyme (MaeB, EC 1.1.1.38-40)

<400> 28
atggatgacc agttaaaaca aagtgcactt gatttccatg aatttccagt tccagggaaa      60

atccaggttt ctccaaccaa gcctctggca acacagcgcg atctggcgct ggcctactca     120

ccaggcgttg ccgcaccttg tcttgaaatc gaaaaagacc cgttaaaagc ctacaaatat     180

accgcccgag gtaacctggt ggcggtgatc tctaacggta cggcggtgct ggggttaggc     240

aacattggcg cgctggcagg caaaccggtg atggaaggca agggcgttct gtttaagaaa     300

ttcgccggga ttgatgtatt tgacattgaa gttgacgaac tcgacccgga caaatttatt     360

gaagttgtcg ccgcgctcga accaaccttc ggcggcatca acctcgaaga cattaaagcg     420

ccagaatgtt tctatattga acagaaactg cgcgagcgga tgaatattcc ggtattccac     480

gacgatcagc acggcacggc aattatcagc actgccgcca tcctcaacgg cttgcgcgtg     540

gtggagaaaa acatctccga cgtgcggatg gtggtttccg gcgcgggtgc cgcagcaatc     600

gcctgtatga acctgctggt agcgctgggt ctgcaaaaac ataacatcgt ggtttgcgat     660

tcaaaaggcg ttatctatca gggccgtgag ccaaacatgg cggaaaccaa agccgcatat     720

gcggtggtgg atgacggcaa acgtaccctc gatgatgtga ttgaaggcgc ggatattttc     780

ctgggctgtt ccggcccgaa agtgctgacc caggaaatgg tgaagaaaat ggctcgtgcg     840

ccaatgatcc tggcgctggc gaacccggaa ccggaaattc tgccgccgct ggcgaaagaa     900

gtgcgtccgg atgccatcat ttgcaccggt cgttctgact atccgaacca ggtgaacaac     960

gtcctgtgct tcccgttcat cttccgtggc gcgctggacg ttggcgcaac cgccatcaac    1020

gaagagatga aactggcggc ggtacgtgcg attgcagaac tcgcccatgc ggaacagagc    1080

gaagtggtgg cttcagcgta tggcgatcag gatctgagct ttggtccgga atacatcatt    1140

ccaaaaccgt ttgatccgcg cttgatcgtt aagatcgctc ctgcggtcgc taaagccgcg    1200

atggagtcgg gcgtggcgac tcgtccgatt gctgatttcg acgtctacat cgacaagctg    1260

actgagttcg tttacaaaac caacctgttt atgaagccga ttttctccca ggctcgcaaa    1320

gcgccgaagc gcgttgttct gccggaaggg gaagaggcgc gcgttctgca tgccactcag    1380

gaactggtaa cgctgggact ggcgaaaccg atccttatcg gtcgtccgaa cgtgatcgaa    1440

atgcgcattc agaaactggg cttgcagatc aaagcgggcg ttgattttga gatcgtcaat    1500

aacgaatccg atccgcgctt taaagagtac tggaccgaat acttccagat catgaagcgt    1560

cgcggcgtca ctcaggaaca ggcgcagcgg gcgctgatca gtaacccgac agtgatcggc    1620

gcgatcatgg ttcagcgtgg ggaagccgat gcaatgattt gcggtacggt gggtgattat    1680

catgaacatt ttagcgtggt gaaaaatgtc tttggttatc gcgatggcgt tcacaccgca    1740

ggtgccatga acgcgctgct gctgccgagt ggtaacacct ttattgccga tacatatgtt    1800

aatgatgaac cggatgcaga agagctggcg gagatcacct tgatggcggc agaaactgtc    1860

cgtcgttttg gtattgagcc gcgcgttgct ttgttgtcgc actccaactt tggttcttct    1920

gactgcccgt cgtcgagcaa aatgcgtcag gcgctggaac tggtcaggga acgtgcacca    1980

gaactgatga ttgatggtga aatgcacggc gatgcagcgc tggtggaagc gattcgcaac    2040

gaccgtatgc cggacagctc tttgaaaggt tccgccaata ttctggtgat gccgaacatg    2100

gaagctgccc gcattagtta caacttactg cgtgtttcca gctcggaagg tgtgactgtc    2160

ggcccggtgc tgatgggtgt ggcgaaaccg gttcacgtgt taacgccgat cgcatcggtg    2220

cgtcgtatcg tcaacatggt ggcgctggcc gtggtagaag cgcaaaccca accgctgtaa    2280


<210> 29
<211> 491
<212> PRT
<213> Escherichia coli

<220> 
<223> glucose 6-phosphate dehydrogenase (Zwf, EC 1.1.1.49)

<400> 29
Met Ala Val Thr Gln Thr Ala Gln Ala Cys Asp Leu Val Ile Phe Gly 
1               5                   10                  15      
Ala Lys Gly Asp Leu Ala Arg Arg Lys Leu Leu Pro Ser Leu Tyr Gln 
            20                  25                  30          
Leu Glu Lys Ala Gly Gln Leu Asn Pro Asp Thr Arg Ile Ile Gly Val 
        35                  40                  45              
Gly Arg Ala Asp Trp Asp Lys Ala Ala Tyr Thr Lys Val Val Arg Glu 
    50                  55                  60                  
Ala Leu Glu Thr Phe Met Lys Glu Thr Ile Asp Glu Gly Leu Trp Asp 
65                  70                  75                  80  
Thr Leu Ser Ala Arg Leu Asp Phe Cys Asn Leu Asp Val Asn Asp Thr 
                85                  90                  95      
Ala Ala Phe Ser Arg Leu Gly Ala Met Leu Asp Gln Lys Asn Arg Ile 
            100                 105                 110         
Thr Ile Asn Tyr Phe Ala Met Pro Pro Ser Thr Phe Gly Ala Ile Cys 
        115                 120                 125             
Lys Gly Leu Gly Glu Ala Lys Leu Asn Ala Lys Pro Ala Arg Val Val 
    130                 135                 140                 
Met Glu Lys Pro Leu Gly Thr Ser Leu Ala Thr Ser Gln Glu Ile Asn 
145                 150                 155                 160 
Asp Gln Val Gly Glu Tyr Phe Glu Glu Cys Gln Val Tyr Arg Ile Asp 
                165                 170                 175     
His Tyr Leu Gly Lys Glu Thr Val Leu Asn Leu Leu Ala Leu Arg Phe 
            180                 185                 190         
Ala Asn Ser Leu Phe Val Asn Asn Trp Asp Asn Arg Thr Ile Asp His 
        195                 200                 205             
Val Glu Ile Thr Val Ala Glu Glu Val Gly Ile Glu Gly Arg Trp Gly 
    210                 215                 220                 
Tyr Phe Asp Lys Ala Gly Gln Met Arg Asp Met Ile Gln Asn His Leu 
225                 230                 235                 240 
Leu Gln Ile Leu Cys Met Ile Ala Met Ser Pro Pro Ser Asp Leu Ser 
                245                 250                 255     
Ala Asp Ser Ile Arg Asp Glu Lys Val Lys Val Leu Lys Ser Leu Arg 
            260                 265                 270         
Arg Ile Asp Arg Ser Asn Val Arg Glu Lys Thr Val Arg Gly Gln Tyr 
        275                 280                 285             
Thr Ala Gly Phe Ala Gln Gly Lys Lys Val Pro Gly Tyr Leu Glu Glu 
    290                 295                 300                 
Glu Gly Ala Asn Lys Ser Ser Asn Thr Glu Thr Phe Val Ala Ile Arg 
305                 310                 315                 320 
Val Asp Ile Asp Asn Trp Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg 
                325                 330                 335     
Thr Gly Lys Arg Leu Pro Thr Lys Cys Ser Glu Val Val Val Tyr Phe 
            340                 345                 350         
Lys Thr Pro Glu Leu Asn Leu Phe Lys Glu Ser Trp Gln Asp Leu Pro 
        355                 360                 365             
Gln Asn Lys Leu Thr Ile Arg Leu Gln Pro Asp Glu Gly Val Asp Ile 
    370                 375                 380                 
Gln Val Leu Asn Lys Val Pro Gly Leu Asp His Lys His Asn Leu Gln 
385                 390                 395                 400 
Ile Thr Lys Leu Asp Leu Ser Tyr Ser Glu Thr Phe Asn Gln Thr His 
                405                 410                 415     
Leu Ala Asp Ala Tyr Glu Arg Leu Leu Leu Glu Thr Met Arg Gly Ile 
            420                 425                 430         
Gln Ala Leu Phe Val Arg Arg Asp Glu Val Glu Glu Ala Trp Lys Trp 
        435                 440                 445             
Val Asp Ser Ile Thr Glu Ala Trp Ala Met Asp Asn Asp Ala Pro Lys 
    450                 455                 460                 
Pro Tyr Gln Ala Gly Thr Trp Gly Pro Val Ala Ser Val Ala Met Ile 
465                 470                 475                 480 
Thr Arg Asp Gly Arg Ser Trp Asn Glu Phe Glu 
                485                 490     

<210> 30
<211> 1476
<212> DNA
<213> Escherichia coli

<220> 
<223> glucose 6-phosphate dehydrogenase (Zwf, EC 1.1.1.49)

<400> 30
atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac      60

cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagccgg tcagctcaac     120

ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa     180

gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac     240

accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcagc     300

cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacca ttaactactt tgccatgccg     360

cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg     420

gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat     480

gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt     540

aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac     600

tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa     660

gggcgctggg gctattttga taaagccggt cagatgcgcg acatgatcca gaaccacctg     720

ctgcaaattc tttgcatgat tgcgatgtct ccgccgtctg acctgagcgc agacagcatc     780

cgcgatgaaa aagtgaaagt actgaagtct ctgcgccgca tcgaccgctc caacgtacgc     840

gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga     900

tatctggaag aagagggcgc gaacaagagc agcaatacag aaactttcgt ggcgatccgc     960

gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt    1020

ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt    1080

aaagaatcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa    1140

ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa    1200

atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc    1260

tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac    1320

gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat    1380

gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt    1440

acccgtgatg gtcgttcctg gaatgagttt gagtaa                              1476


<210> 31
<211> 117
<212> DNA
<213> Artificial Sequence

<220> 
<223> Promoter of mutated pntAB

<400> 31
tagggtgttt gttactaatt tattttaacg gagtaacatt tagctcgtac atgagcagct     60

tgtgtggctc ctgacacagg caaaccatca tcaataaaac cgatggaagg gaatatc       117


<210> 32
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> UTR of mutated fdh

<400> 32
caggacgcaa tgaccgaatt cactagttaa tagaaataat tttgtttaac tttaaggagg     60

tttgga                                                                66


<210> 33
<211> 339
<212> PRT
<213> Bacillus stearothermophilus 

<220> 
<223> methanol dehydrogenases

<400> 33
Met Lys Ala Ala Val Val Asn Glu Phe Lys Lys Ala Leu Glu Ile Lys 
1               5                   10                  15      
Glu Val Glu Arg Pro Lys Leu Glu Glu Gly Glu Val Leu Val Lys Ile 
            20                  25                  30          
Glu Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp 
        35                  40                  45              
Trp Pro Ile Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val 
    50                  55                  60                  
Gly Ile Val Val Glu Val Ala Lys Gly Val Lys Ser Ile Lys Val Gly 
65                  70                  75                  80  
Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly Glu Cys Glu 
                85                  90                  95      
Tyr Cys Leu Thr Gly Gln Glu Thr Leu Cys Pro His Gln Leu Asn Gly 
            100                 105                 110         
Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Lys Ala Pro Ala 
        115                 120                 125             
Asp Tyr Val Ala Lys Ile Pro Asp Asn Leu Asp Pro Val Glu Val Ala 
    130                 135                 140                 
Pro Ile Leu Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Ser 
145                 150                 155                 160 
Gly Ala Arg Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu 
                165                 170                 175     
Gly His Ile Ala Leu Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val 
            180                 185                 190         
Ala Val Asp Ile Ser Asp Glu Lys Ser Lys Leu Ala Lys Asp Leu Gly 
        195                 200                 205             
Ala Asp Ile Ala Ile Asn Gly Leu Lys Glu Asp Pro Val Lys Ala Ile 
    210                 215                 220                 
His Asp Gln Val Gly Gly Val His Ala Ala Ile Ser Val Ala Val Asn 
225                 230                 235                 240 
Lys Lys Ala Phe Glu Gln Ala Tyr Gln Ser Val Lys Arg Gly Gly Thr 
                245                 250                 255     
Leu Val Val Val Gly Leu Pro Asn Ala Asp Leu Pro Ile Pro Ile Phe 
            260                 265                 270         
Asp Thr Val Leu Asn Gly Val Ser Val Lys Gly Ser Ile Val Gly Thr 
        275                 280                 285             
Arg Lys Asp Met Gln Glu Ala Leu Asp Phe Ala Ala Arg Gly Lys Val 
    290                 295                 300                 
Arg Pro Ile Val Glu Thr Ala Glu Leu Glu Glu Ile Asn Glu Val Phe 
305                 310                 315                 320 
Glu Arg Met Glu Lys Gly Lys Ile Asn Gly Arg Ile Val Leu Lys Leu 
                325                 330                 335     
Lys Glu Asp 
            

<210> 34
<211> 1020
<212> DNA
<213> Bacillus stearothermophilus 

<220> 
<223> methanol dehydrogenases

<400> 34
atgaaagctg ctgttgttaa cgaatttaaa aaagctctgg aaatcaaaga agttgaacgt      60

ccgaaactgg aagaaggtga agttctggtt aaaatcgaag cttgcggtgt ttgccacacc     120

gacctgcacg ctgctcacgg tgactggccg atcaaaccga aactgccgct gatcccgggt     180

cacgaaggtg ttggtatcgt tgttgaagtt gctaaaggtg ttaaatctat caaagttggt     240

gaccgtgttg gtatcccgtg gctgtactct gcttgcggtg aatgcgaata ctgcctgacc     300

ggtcaggaaa ccctgtgccc gcaccagctg aacggtggtt actctgttga cggtggttac     360

gctgaatact gcaaagctcc ggctgactac gttgctaaaa tcccggacaa cctggacccg     420

gttgaagttg ctccgatcct gtgcgctggt gttaccacct acaaagctct gaaagtttct     480

ggtgctcgtc cgggtgaatg ggttgctatc tacggtatcg gtggtctggg tcacatcgct     540

ctgcaatacg ctaaagctat gggtctgaac gttgttgctg ttgacatctc tgacgaaaaa     600

tctaaactgg ctaaagacct gggtgctgac atcgctatca acggtctgaa agaagacccg     660

gttaaagcta tccacgacca ggttggtggt gttcacgctg ctatctctgt tgctgttaac     720

aaaaaagctt tcgaacaggc ttaccagtct gttaaacgtg gtggtaccct ggttgttgtt     780

ggtctgccga acgctgacct gccgatcccg atcttcgaca ccgttctgaa cggtgtttct     840

gttaaaggtt ctatcgttgg tacccgtaaa gacatgcagg aagctctgga cttcgctgct     900

cgtggtaaag ttcgtccgat agttgaaacc gctgaactgg aagaaatcaa cgaagttttc     960

gaacgtatgg aaaaaggtaa aatcaacggt cgtatcgttc tgaaactgaa agaagactaa    1020


<210> 35
<211> 390
<212> PRT
<213> Cupriavidus necator

<220> 
<223> methanol dehydrogenases

<400> 35
Met Thr His Leu Asn Ile Ala Asn Arg Val Asp Ser Phe Phe Ile Pro 
1               5                   10                  15      
Cys Val Thr Leu Phe Gly Pro Gly Cys Ala Arg Glu Thr Gly Ala Arg 
            20                  25                  30          
Ala Arg Ser Leu Gly Ala Arg Lys Ala Leu Ile Val Thr Asp Ala Gly 
        35                  40                  45              
Leu His Lys Met Gly Leu Ser Glu Val Val Ala Gly His Ile Arg Glu 
    50                  55                  60                  
Ala Gly Leu Gln Ala Val Ile Phe Pro Gly Ala Glu Pro Asn Pro Thr 
65                  70                  75                  80  
Asp Val Asn Val His Asp Gly Val Lys Leu Phe Glu Arg Glu Glu Cys 
                85                  90                  95      
Asp Phe Ile Val Ser Leu Gly Gly Gly Ser Ser His Asp Cys Ala Lys 
            100                 105                 110         
Gly Ile Gly Leu Val Thr Ala Gly Gly Gly His Ile Arg Asp Tyr Glu 
        115                 120                 125             
Gly Ile Asp Lys Ser Thr Val Pro Met Thr Pro Leu Ile Ser Ile Asn 
    130                 135                 140                 
Thr Thr Ala Gly Thr Ala Ala Glu Met Thr Arg Phe Cys Ile Ile Thr 
145                 150                 155                 160 
Asn Ser Ser Asn His Val Lys Met Ala Ile Val Asp Trp Arg Cys Thr 
                165                 170                 175     
Pro Leu Ile Ala Ile Asp Asp Pro Ser Leu Met Val Ala Met Pro Pro 
            180                 185                 190         
Ala Leu Thr Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Ile Glu 
        195                 200                 205             
Ala Tyr Val Ser Thr Ala Ala Thr Pro Ile Thr Asp Ala Cys Ala Glu 
    210                 215                 220                 
Lys Ala Ile Val Leu Ile Ala Glu Trp Leu Pro Lys Ala Val Ala Asn 
225                 230                 235                 240 
Gly Asp Ser Met Glu Ala Arg Ala Ala Met Cys Tyr Ala Gln Tyr Leu 
                245                 250                 255     
Ala Gly Met Ala Phe Asn Asn Ala Ser Leu Gly Tyr Val His Ala Met 
            260                 265                 270         
Ala His Gln Leu Gly Gly Phe Tyr Asn Leu Pro His Gly Val Cys Asn 
        275                 280                 285             
Ala Ile Leu Leu Pro His Val Ser Glu Phe Asn Leu Ile Ala Ala Pro 
    290                 295                 300                 
Glu Arg Tyr Ala Arg Ile Ala Glu Leu Leu Gly Glu Asn Ile Gly Gly 
305                 310                 315                 320 
Leu Ser Ala His Asp Ala Ala Lys Ala Ala Val Ser Ala Ile Arg Thr 
                325                 330                 335     
Leu Ser Thr Ser Ile Gly Ile Pro Ala Gly Leu Ala Gly Leu Gly Val 
            340                 345                 350         
Lys Ala Asp Asp His Glu Val Met Ala Ser Asn Ala Gln Lys Asp Ala 
        355                 360                 365             
Cys Met Leu Thr Asn Pro Arg Lys Ala Thr Leu Ala Gln Val Met Ala 
    370                 375                 380                 
Ile Phe Ala Ala Ala Met 
385                 390 

<210> 36
<211> 1173
<212> DNA
<213> Cupriavidus necator

<220> 
<223> methanol dehydrogenases

<400> 36
atgacccacc tgaacatcgc taatcgcgtc gacagcttct tcattccctg cgtgaccctc      60

ttcggtccgg gctgcgcgcg cgaaacgggc gctcgcgcca gatcactcgg ggccaggaag     120

gctctcatcg tcacggatgc aggcttgcac aagatggggc tctccgaagt cgtcgcgggg     180

cacattcgcg aagccgggct ccaggccgtc atctttccgg gtgccgagcc caatcccacc     240

gacgttaacg ttcacgacgg cgtcaagttg ttcgagcggg aagaatgcga cttcatcgtt     300

tcgctcggcg gcggctcatc gcacgactgc gcgaaaggca tcggcctcgt taccgccgga     360

ggcggacata tccgcgacta cgaaggcatc gacaaatcaa cggtgccaat gacgccgctg     420

atttcgatca acacgaccgc tggcactgct gcggaaatga cacgcttttg catcatcact     480

aattcgagca atcatgtgaa gatggcaatc gtcgactggc gttgcacgcc attaatcgcc     540

atcgacgatc cgagcctgat ggtcgcgatg ccgcccgcct tgacggcggc gaccggcatg     600

gacgcgttga ctcacgccat cgaggcatac gtttccaccg ccgccacgcc aattaccgat     660

gcctgtgcgg agaaggcgat cgtgctgatc gccgaatggc tgcccaaagc tgtcgcgaac     720

ggggactcga tggaagcacg cgcggccatg tgctacgccc aataccttgc cggcatggcc     780

ttcaacaacg catcactcgg ttacgtgcac gcgatggccc atcaactcgg cggcttctac     840

aatttgcccc acggcgtgtg caacgcgatc ctgctgccgc acgtgtcgga attcaacctc     900

attgccgcgc cggagcgcta cgcgagaatc gccgaactgc taggcgagaa cattgggggc     960

ttgagcgcgc atgacgccgc caaagctgcc gtctcggcga tccggaccct ttccacgtcg    1020

attggcattc cggcgggtct ggcgggcctg ggcgtcaagg cggacgacca tgaagtgatg    1080

gcaagcaatg cgcaaaagga tgcttgcatg ctgacgaatc cgcgcaaggc cacgctggcg    1140

caagtcatgg caatcttcgc tgcggcgatg taa                                 1173


<210> 37
<211> 345
<212> PRT
<213> Corynebacterium glutamicum

<220> 
<223> methanol dehydrogenases

<400> 37
Met Thr Thr Ala Ala Pro Gln Glu Phe Thr Ala Ala Val Val Glu Lys 
1               5                   10                  15      
Phe Gly His Glu Val Thr Val Lys Asp Ile Asp Leu Pro Lys Pro Gly 
            20                  25                  30          
Pro Asn Gln Ala Leu Val Lys Val Leu Thr Ser Gly Ile Cys His Thr 
        35                  40                  45              
Asp Leu His Ala Leu Glu Gly Asp Trp Pro Val Lys Pro Glu Pro Pro 
    50                  55                  60                  
Phe Val Pro Gly His Glu Gly Val Gly Glu Val Val Glu Leu Gly Pro 
65                  70                  75                  80  
Gly Glu His Asp Val Lys Val Gly Asp Ile Val Gly Asn Ala Trp Leu 
                85                  90                  95      
Trp Ser Ala Cys Gly Thr Cys Glu Tyr Cys Ile Thr Gly Arg Glu Thr 
            100                 105                 110         
Gln Cys Asn Glu Ala Glu Tyr Gly Gly Tyr Thr Gln Asn Gly Ser Phe 
        115                 120                 125             
Gly Gln Tyr Met Leu Val Asp Thr Arg Tyr Ala Ala Arg Ile Pro Asp 
    130                 135                 140                 
Gly Val Asp Tyr Leu Glu Ala Ala Pro Ile Leu Cys Ala Gly Val Thr 
145                 150                 155                 160 
Val Tyr Lys Ala Leu Lys Val Ser Glu Thr Arg Pro Gly Gln Phe Met 
                165                 170                 175     
Val Ile Ser Gly Val Gly Gly Leu Gly His Ile Ala Val Gln Tyr Ala 
            180                 185                 190         
Ala Ala Met Gly Met Arg Val Ile Ala Val Asp Ile Ala Asp Asp Lys 
        195                 200                 205             
Leu Glu Leu Ala Arg Lys His Gly Ala Glu Phe Thr Val Asn Ala Arg 
    210                 215                 220                 
Asn Glu Asp Pro Gly Glu Ala Val Gln Lys Tyr Thr Asn Gly Gly Ala 
225                 230                 235                 240 
His Gly Val Leu Val Thr Ala Val His Glu Ala Ala Phe Gly Gln Ala 
                245                 250                 255     
Leu Asp Met Ala Arg Arg Ala Gly Thr Ile Val Phe Asn Gly Leu Pro 
            260                 265                 270         
Pro Gly Glu Phe Pro Ala Ser Val Phe Asn Ile Val Phe Lys Gly Leu 
        275                 280                 285             
Thr Ile Arg Gly Ser Leu Val Gly Thr Arg Gln Asp Leu Ala Glu Ala 
    290                 295                 300                 
Leu Asp Phe Phe Ala Arg Gly Leu Ile Lys Pro Thr Val Ser Glu Cys 
305                 310                 315                 320 
Ser Leu Asp Glu Val Asn Asp Val Leu Asp Arg Met Arg Asn Gly Lys 
                325                 330                 335     
Ile Asp Gly Arg Val Ala Ile Arg Tyr 
            340                 345 

<210> 38
<211> 1038
<212> DNA
<213> Corynebacterium glutamicum

<220> 
<223> methanol dehydrogenases

<400> 38
atgaccaccg ctgctccgca ggaatttacc gctgctgttg ttgaaaaatt cggtcacgaa      60

gttaccgtta aagacatcga cctgccgaaa ccgggtccga accaggctct ggttaaagtt     120

ctgacctctg gtatctgcca caccgacctg cacgctctgg aaggtgactg gccggttaaa     180

ccggaaccgc cgttcgttcc gggtcacgaa ggtgttggtg aagttgttga actgggtccg     240

ggtgaacacg acgttaaagt tggtgacatc gttggtaacg cttggctgtg gtctgcttgc     300

ggtacctgcg aatactgcat caccggtcgt gaaacccagt gcaacgaagc tgaatacggt     360

ggttacaccc agaacggttc tttcggtcag tacatgctgg ttgacacccg ttacgctgct     420

cgtatcccgg acggtgttga ctacctggaa gctgctccga tcctgtgcgc tggtgttacc     480

gtttacaaag ctctgaaagt ttctgaaacc cgtccgggtc agttcatggt tatctctggt     540

gttggtggtc tgggtcacat cgctgttcag tacgctgctg ctatgggtat gcgtgttatc     600

gctgttgaca tcgctgacga caaactggaa ctggctcgta aacacggtgc tgaatttacc     660

gttaacgctc gtaacgaaga cccgggtgaa gctgttcaga aatacaccaa cggtggtgct     720

cacggtgttc tggttaccgc tgttcacgaa gctgctttcg gtcaggctct ggacatggct     780

cgtcgtgctg gtaccatcgt tttcaacggt ctgccgccgg gtgaatttcc ggcttctgtt     840

ttcaacatcg ttttcaaagg tctgaccatc cgtggttctc tggttggtac ccgtcaggac     900

ctggctgaag ctctggactt cttcgctcgt ggtctgatca aaccgaccgt ttctgaatgc     960

tctctggacg aagttaacga cgttctggac cgtatgcgta acggtaaaat cgacggtcgt    1020

gttgctatcc gttactaa                                                  1038


<210> 39
<211> 385
<212> PRT
<213> Bacillus methanolicus

<220> 
<223> methanol dehydrogenases

<400> 39
Met Thr Asn Thr Gln Ser Ala Phe Phe Met Pro Ser Val Asn Leu Phe 
1               5                   10                  15      
Gly Ala Gly Ser Val Asn Glu Val Gly Thr Arg Leu Ala Asp Leu Gly 
            20                  25                  30          
Val Lys Lys Ala Leu Leu Val Thr Asp Ala Gly Leu His Gly Leu Gly 
        35                  40                  45              
Leu Ser Glu Lys Ile Ser Ser Ile Ile Arg Ala Ala Gly Val Glu Val 
    50                  55                  60                  
Ser Ile Phe Pro Lys Ala Glu Pro Asn Pro Thr Asp Lys Asn Val Ala 
65                  70                  75                  80  
Glu Gly Leu Glu Ala Tyr Asn Ala Glu Asn Cys Asp Ser Ile Val Thr 
                85                  90                  95      
Leu Gly Gly Gly Ser Ser His Asp Ala Gly Lys Ala Ile Ala Leu Val 
            100                 105                 110         
Ala Ala Asn Gly Gly Lys Ile His Asp Tyr Glu Gly Val Asp Val Ser 
        115                 120                 125             
Lys Glu Pro Met Val Pro Leu Ile Ala Ile Asn Thr Thr Ala Gly Thr 
    130                 135                 140                 
Gly Ser Glu Leu Thr Lys Phe Thr Ile Ile Thr Asp Thr Glu Arg Lys 
145                 150                 155                 160 
Val Lys Met Ala Ile Val Asp Lys His Val Thr Pro Thr Leu Ser Ile 
                165                 170                 175     
Asn Asp Pro Glu Leu Met Val Gly Met Pro Pro Ser Leu Thr Ala Ala 
            180                 185                 190         
Thr Gly Leu Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Thr 
        195                 200                 205             
Gly Ala Thr Pro Ile Thr Asp Ala Leu Ala Ile Gln Ala Ile Lys Ile 
    210                 215                 220                 
Ile Ser Lys Tyr Leu Pro Arg Ala Val Ala Asn Gly Lys Asp Ile Glu 
225                 230                 235                 240 
Ala Arg Glu Gln Met Ala Phe Ala Gln Ser Leu Ala Gly Met Ala Phe 
                245                 250                 255     
Asn Asn Ala Gly Leu Gly Tyr Val His Ala Ile Ala His Gln Leu Gly 
            260                 265                 270         
Gly Phe Tyr Asn Phe Pro His Gly Val Cys Asn Ala Val Leu Leu Pro 
        275                 280                 285             
Tyr Val Cys Arg Phe Asn Leu Ile Ser Lys Val Glu Arg Tyr Ala Glu 
    290                 295                 300                 
Ile Ala Ala Phe Leu Gly Glu Asn Val Asp Gly Leu Ser Thr Tyr Asp 
305                 310                 315                 320 
Ala Ala Glu Lys Ala Ile Lys Ala Ile Glu Arg Met Ala Lys Asp Leu 
                325                 330                 335     
Asn Ile Pro Lys Gly Phe Lys Glu Leu Gly Ala Lys Glu Glu Asp Ile 
            340                 345                 350         
Glu Thr Leu Ala Lys Asn Ala Met Lys Asp Ala Cys Ala Leu Thr Asn 
        355                 360                 365             
Pro Arg Lys Pro Lys Leu Glu Glu Val Ile Gln Ile Ile Lys Asn Ala 
    370                 375                 380                 
Met 
385 

<210> 40
<211> 1158
<212> DNA
<213> Bacillus methanolicus

<220> 
<223> methanol dehydrogenases

<400> 40
atgaccaaca cccagtctgc tttcttcatg ccgtctgtta acctgttcgg tgctggttct      60

gttaacgaag ttggtacccg tctggctgac ctgggtgtta aaaaagctct gctggttacc     120

gacgctggtc tgcacggtct gggtctgtct gaaaaaatct cttctatcat ccgtgctgct     180

ggtgttgaag tttctatctt cccgaaagct gaaccgaacc cgaccgacaa aaacgttgct     240

gaaggtctgg aagcttacaa cgctgaaaac tgcgactcta tcgttaccct gggtggtggt     300

tcttctcacg acgctggtaa agctatcgct ctggttgctg ctaacggtgg taaaatccac     360

gactacgaag gtgttgacgt ttctaaagaa ccgatggttc cgctgatcgc tatcaacacc     420

accgctggta ccggttctga actgaccaaa ttcaccatca tcaccgacac cgaacgtaaa     480

gttaaaatgg ctatcgttga caaacacgtt accccgaccc tgtctatcaa cgacccggaa     540

ctgatggttg gtatgccgcc gtctctgacc gctgctaccg gtctggacgc tctgacccac     600

gctatcgaag cttacgtttc taccggtgct accccgatca ccgacgctct ggctatccag     660

gctatcaaaa tcatctctaa atacctgccg cgtgctgttg ctaacggtaa agacatcgaa     720

gctcgtgaac agatggcttt cgctcagtct ctggctggta tggctttcaa caacgctggt     780

ctgggttacg ttcacgctat cgctcaccag ctgggtggtt tctacaactt cccgcacggt     840

gtttgcaacg ctgttctgct gccgtacgtt tgccgtttca acctgatctc taaagttgaa     900

cgttacgctg aaatcgctgc tttcctgggt gaaaacgttg acggtctgtc tacctacgac     960

gctgctgaaa aagctatcaa agctatcgaa cgtatggcta aagacctgaa catcccgaaa    1020

ggtttcaaag aactgggtgc taaagaagaa gacatcgaaa ccctggctaa aaacgctatg    1080

aaagacgctt gcgctctgac caacccgcgt aaaccgaaac tggaagaagt tatccagatc    1140

atcaaaaacg ctatgtaa                                                  1158


<210> 41
<211> 385
<212> PRT
<213> Bacillus methanolicus

<220> 
<223> methanol dehydrogenases

<400> 41
Met Thr Asn Thr Leu Ser Ala Phe Phe Met Pro Ser Val Asn Leu Phe 
1               5                   10                  15      
Gly Ala Gly Ser Val Asn Glu Val Gly Thr Arg Leu Ala Asp Leu Gly 
            20                  25                  30          
Val Lys Lys Ala Leu Leu Val Thr Asp Ala Gly Leu His Gly Leu Gly 
        35                  40                  45              
Leu Ser Glu Lys Ile Ser Ser Ile Ile Arg Ala Ala Gly Val Glu Val 
    50                  55                  60                  
Ser Ile Phe Pro Lys Ala Glu Pro Asn Pro Thr Asp Lys Asn Val Ala 
65                  70                  75                  80  
Glu Gly Leu Glu Ala Tyr Asn Ala Glu Asn Cys Asp Ser Ile Val Thr 
                85                  90                  95      
Leu Gly Gly Gly Ser Ser His Asp Ala Gly Lys Ala Ile Ala Leu Val 
            100                 105                 110         
Ala Ala Asn Gly Gly Lys Ile His Asp Tyr Glu Gly Val Asp Val Ser 
        115                 120                 125             
Lys Glu Pro Met Val Pro Leu Ile Ala Ile Asn Thr Thr Ala Gly Thr 
    130                 135                 140                 
Gly Ser Glu Leu Thr Lys Phe Thr Ile Ile Thr Asp Thr Glu Arg Lys 
145                 150                 155                 160 
Val Lys Met Ala Ile Val Asp Lys His Val Thr Pro Thr Leu Ser Ile 
                165                 170                 175     
Asn Asp Pro Glu Leu Met Val Gly Met Pro Pro Ser Leu Thr Ala Ala 
            180                 185                 190         
Thr Gly Leu Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Thr 
        195                 200                 205             
Gly Ala Thr Pro Ile Thr Asp Ala Leu Ala Ile Gln Ala Ile Lys Ile 
    210                 215                 220                 
Ile Ser Lys Tyr Leu Pro Arg Ala Val Ala Asn Gly Lys Asp Ile Glu 
225                 230                 235                 240 
Ala Arg Glu Gln Met Ala Phe Ala Gln Ser Leu Ala Gly Met Ala Phe 
                245                 250                 255     
Asn Asn Ala Gly Leu Gly Tyr Val His Ala Ile Ala His Gln Leu Gly 
            260                 265                 270         
Gly Phe Tyr Asn Phe Pro His Gly Val Cys Asn Ala Val Leu Leu Pro 
        275                 280                 285             
Tyr Val Cys Arg Phe Asn Leu Ile Ser Lys Val Glu Arg Tyr Ala Glu 
    290                 295                 300                 
Ile Ala Ala Phe Leu Gly Glu Asn Val Asp Gly Leu Ser Thr Tyr Asp 
305                 310                 315                 320 
Ala Ala Glu Lys Ala Ile Lys Ala Ile Glu Arg Met Ala Lys Asp Leu 
                325                 330                 335     
Asn Ile Pro Lys Gly Phe Lys Glu Leu Gly Ala Lys Glu Glu Asp Ile 
            340                 345                 350         
Glu Thr Leu Ala Lys Asn Ala Met Lys Asp Leu Cys Ala Leu Thr Asn 
        355                 360                 365             
Pro Arg Lys Pro Lys Leu Glu Glu Val Ile Gln Ile Ile Lys Asn Ala 
    370                 375                 380                 
Met 
385 

<210> 42
<211> 1158
<212> DNA
<213> Bacillus methanolicus

<220> 
<223> methanol dehydrogenases

<400> 42
atgaccaaca ccctgtctgc tttcttcatg ccgtctgtta acctgttcgg tgctggttct      60

gttaacgaag ttggtacccg tctggctgac ctgggtgtta aaaaagctct gctggttacc     120

gacgctggtc tgcacggtct gggtctgtct gaaaaaatct cttctatcat ccgtgctgct     180

ggtgttgaag tttctatctt cccgaaagct gaaccgaacc cgaccgacaa aaacgttgct     240

gaaggtctgg aagcttacaa cgctgaaaac tgcgactcta tcgttaccct gggtggtggt     300

tcttctcacg acgctggtaa agctatcgct ctggttgctg ctaacggtgg taaaatccac     360

gactacgaag gtgttgacgt ttctaaagaa ccgatggttc cgctgatcgc tatcaacacc     420

accgctggta ccggttctga actgaccaaa ttcaccatca tcaccgacac cgaacgtaaa     480

gttaaaatgg ctatcgttga caaacacgtt accccgaccc tgtctatcaa cgacccggaa     540

ctgatggttg gtatgccgcc gtctctgacc gctgctaccg gtctggacgc tctgacccac     600

gctatcgaag cttacgtttc taccggtgct accccgatca ccgacgctct ggctatccag     660

gctatcaaaa tcatctctaa atacctgccg cgtgctgttg ctaacggtaa agacatcgaa     720

gctcgtgaac agatggcttt cgctcagtct ctggctggta tggctttcaa caacgctggt     780

ctgggttacg ttcacgctat cgctcaccag ctgggtggtt tctacaactt cccgcacggt     840

gtttgcaacg ctgttctgct gccgtacgtt tgccgtttca acctgatctc taaagttgaa     900

cgttacgctg aaatcgctgc tttcctgggt gaaaacgttg acggtctgtc tacctacgac     960

gctgctgaaa aagctatcaa agctatcgaa cgtatggcta aagacctgaa catcccgaaa    1020

ggtttcaaag aactgggtgc taaagaagaa gacatcgaaa ccctggctaa aaacgctatg    1080

aaagacctgt gcgctctgac caacccgcgt aaaccgaaac tggaagaagt tatccagatc    1140

atcaaaaacg ctatgtaa                                                  1158


<210> 43
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-A

<400> 43
aggaggtttg ga                                                        12


<210> 44
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-B

<400> 44
aacaaaatga ggaggtactg ag                                             22


<210> 45
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-C

<400> 45
aagttaagag gcaaga                                                    16


<210> 46
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-D

<400> 46
ttcgcagggg gaag                                                      14


<210> 47
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-E

<400> 47
taagcaggac cggcggcg                                                  18


<210> 48
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> RBS-F

<400> 48
caccatacac tg                                                        12


<210> 49
<211> 399
<212> PRT
<213> Pseudomonas putida

<220> 
<223> Formaldehyde dehydrogenases

<400> 49
Met Ser Gly Asn Arg Gly Val Val Tyr Leu Gly Ser Gly Lys Val Glu 
1               5                   10                  15      
Val Gln Lys Ile Asp Tyr Pro Lys Met Gln Asp Pro Arg Gly Lys Lys 
            20                  25                  30          
Ile Glu His Gly Val Ile Leu Lys Val Val Ser Thr Asn Ile Cys Gly 
        35                  40                  45              
Ser Asp Gln His Met Val Arg Gly Arg Thr Thr Ala Gln Val Gly Leu 
    50                  55                  60                  
Val Leu Gly His Glu Ile Thr Gly Glu Val Ile Glu Lys Gly Arg Asp 
65                  70                  75                  80  
Val Glu Asn Leu Gln Ile Gly Asp Leu Val Ser Val Pro Phe Asn Val 
                85                  90                  95      
Ala Cys Gly Arg Cys Arg Ser Cys Lys Glu Met His Thr Gly Val Cys 
            100                 105                 110         
Leu Thr Val Asn Pro Ala Arg Ala Gly Gly Ala Tyr Gly Tyr Val Asp 
        115                 120                 125             
Met Gly Asp Trp Thr Gly Gly Gln Ala Glu Tyr Leu Leu Val Pro Tyr 
    130                 135                 140                 
Ala Asp Phe Asn Leu Leu Lys Leu Pro Asp Arg Asp Lys Ala Met Glu 
145                 150                 155                 160 
Lys Ile Arg Asp Leu Thr Cys Leu Ser Asp Ile Leu Pro Thr Gly Tyr 
                165                 170                 175     
His Gly Ala Val Thr Ala Gly Val Gly Pro Gly Ser Thr Val Tyr Val 
            180                 185                 190         
Ala Gly Ala Gly Pro Val Gly Leu Ala Ala Ala Ala Ser Ala Arg Leu 
        195                 200                 205             
Leu Gly Ala Ala Val Val Ile Val Gly Asp Leu Asn Pro Ala Arg Leu 
    210                 215                 220                 
Ala His Ala Lys Ala Gln Gly Phe Glu Ile Ala Asp Leu Ser Leu Asp 
225                 230                 235                 240 
Thr Pro Leu His Glu Gln Ile Ala Ala Leu Leu Gly Glu Pro Glu Val 
                245                 250                 255     
Asp Cys Ala Val Asp Ala Val Gly Phe Glu Ala Arg Gly His Gly His 
            260                 265                 270         
Glu Gly Ala Lys His Glu Ala Pro Ala Thr Val Leu Asn Ser Leu Met 
        275                 280                 285             
Gln Val Thr Arg Val Ala Gly Lys Ile Gly Ile Pro Gly Leu Tyr Val 
    290                 295                 300                 
Thr Glu Asp Pro Gly Ala Val Asp Ala Ala Ala Lys Ile Gly Ser Leu 
305                 310                 315                 320 
Ser Ile Arg Phe Gly Leu Gly Trp Ala Lys Ser His Ser Phe His Thr 
                325                 330                 335     
Gly Gln Thr Pro Val Met Lys Tyr Asn Arg Ala Leu Met Gln Ala Ile 
            340                 345                 350         
Met Trp Asp Arg Ile Asn Ile Ala Glu Val Val Gly Val Gln Val Ile 
        355                 360                 365             
Ser Leu Asp Asp Ala Pro Arg Gly Tyr Gly Glu Phe Asp Ala Gly Val 
    370                 375                 380                 
Pro Lys Lys Phe Val Ile Asp Pro His Lys Thr Phe Ser Ala Ala 
385                 390                 395                 

<210> 50
<211> 1200
<212> DNA
<213> Pseudomonas putida

<220> 
<223> Formaldehyde dehydrogenases

<400> 50
atgtctggta accgtggtgt tgtttacctg ggttctggta aagttgaagt tcagaaaatc      60

gactacccga aaatgcagga cccgcgtggt aaaaaaatcg aacacggtgt tatcctgaaa     120

gttgtttcta ccaacatctg cggttctgac cagcacatgg ttcgtggtcg taccaccgct     180

caggttggtc tggttctggg tcacgaaatc accggtgaag ttatcgaaaa aggtcgtgac     240

gttgaaaacc tgcaaatcgg tgacctggtt tctgttccgt tcaacgttgc ttgcggtcgt     300

tgccgttctt gcaaagaaat gcacaccggt gtttgcctga ccgttaaccc ggctcgtgct     360

ggtggtgctt acggttacgt tgacatgggt gactggaccg gtggtcaggc tgaatacctg     420

ctggttccgt acgctgactt caacctgctg aaactgccgg accgtgacaa agctatggaa     480

aaaatccgtg acctgacctg cctgtctgac atcctgccga ccggttacca cggtgctgtt     540

accgctggtg ttggtccggg ttctaccgtt tacgttgctg gtgctggtcc ggttggtctg     600

gctgctgctg cttctgctcg tctgctgggt gctgctgttg ttatcgttgg tgacctgaac     660

ccggctcgtc tggctcacgc taaagctcag ggtttcgaaa tcgctgacct gtctctggac     720

accccgctgc acgaacagat cgctgctctg ctgggtgaac cggaagttga ctgcgctgtt     780

gacgctgttg gtttcgaagc tcgtggtcac ggtcacgaag gtgctaaaca cgaagctccg     840

gctaccgttc tgaactctct gatgcaggtt acccgtgttg ctggtaaaat cggtatcccg     900

ggtctgtacg ttaccgaaga cccgggtgct gttgacgctg ctgctaaaat cggttctctg     960

tctatccgtt tcggtctggg ttgggctaaa tctcactctt tccacaccgg tcagaccccg    1020

gttatgaaat acaaccgtgc tctgatgcag gctatcatgt gggaccgtat caacatcgct    1080

gaagttgttg gtgttcaggt tatctctctg gacgacgctc cgcgtggtta cggtgaattt    1140

gacgctggtg ttccgaaaaa attcgttatc gacccgcaca aaaccttctc tgctgcttaa    1200


<210> 51
<211> 399
<212> PRT
<213> Pseudomonas aeruginosa

<220> 
<223> Formaldehyde dehydrogenases

<400> 51
Met Ser Gly Asn Arg Gly Val Val Tyr Leu Gly Pro Gly Lys Val Glu 
1               5                   10                  15      
Val Gln Asn Ile Pro Tyr Pro Lys Met Gln Asp Pro Gln Gly Arg Gln 
            20                  25                  30          
Ile Asp His Gly Val Ile Leu Arg Val Val Ser Thr Asn Ile Cys Gly 
        35                  40                  45              
Ser Asp Gln His Met Val Arg Gly Arg Thr Thr Ala Pro Glu Gly Leu 
    50                  55                  60                  
Val Leu Gly His Glu Ile Thr Gly Glu Val Val Glu Ile Gly Arg Gly 
65                  70                  75                  80  
Val Glu Thr Met Lys Ile Gly Asp Leu Val Ser Val Pro Phe Asn Val 
                85                  90                  95      
Ala Cys Gly His Cys Arg Thr Cys Lys Glu Gln His Thr Gly Val Cys 
            100                 105                 110         
Leu Thr Val Asn Pro Ala Arg Ala Gly Gly Ala Tyr Gly Tyr Val Asp 
        115                 120                 125             
Met Gly Gly Trp Val Gly Gly Gln Ala Glu Tyr Val Leu Val Pro Tyr 
    130                 135                 140                 
Ala Asp Phe Asn Leu Leu Lys Leu Pro Asn Arg Glu Ala Ala Met Glu 
145                 150                 155                 160 
Lys Ile Arg Asp Leu Thr Cys Leu Ser Asp Ile Leu Pro Thr Gly Tyr 
                165                 170                 175     
His Gly Ala Val Thr Ala Gly Val Gly Pro Gly Ser Thr Val Tyr Ile 
            180                 185                 190         
Ala Gly Ala Gly Pro Val Gly Leu Ala Ala Ala Ala Ser Ala Arg Leu 
        195                 200                 205             
Leu Gly Ala Ala Val Val Ile Val Gly Asp Val Asn Pro Thr Arg Leu 
    210                 215                 220                 
Ala His Ala Lys Lys Gln Gly Phe Glu Ile Ala Asp Leu Ser Lys Asp 
225                 230                 235                 240 
Thr Pro Leu His Glu Gln Ile Ala Ala Leu Leu Gly Glu Pro Glu Val 
                245                 250                 255     
Asp Cys Ala Val Asp Ala Val Gly Phe Glu Ala Arg Gly His Gly His 
            260                 265                 270         
Ser Gly Ser Gln Gln Glu Ala Pro Ala Thr Val Leu Asn Ser Leu Met 
        275                 280                 285             
Gly Ile Thr Arg Val Ala Gly Lys Ile Gly Ile Pro Gly Leu Tyr Val 
    290                 295                 300                 
Thr Glu Asp Pro Gly Ala Val Asp Ala Ala Ala Lys His Gly Ala Leu 
305                 310                 315                 320 
Ser Ile Arg Phe Gly Leu Gly Trp Ala Lys Ser His Ser Phe His Thr 
                325                 330                 335     
Gly Gln Thr Pro Val Met Lys Tyr Asn Arg Gln Leu Met Gln Ala Ile 
            340                 345                 350         
Met Trp Asp Arg Ile Lys Ile Ala Asp Ile Val Gly Val Glu Val Ile 
        355                 360                 365             
Thr Leu Asp Asp Ala Pro Lys Gly Tyr Gly Glu Phe Asp Ala Gly Val 
    370                 375                 380                 
Pro Lys Lys Phe Val Ile Asp Pro His Asn Leu Phe Arg Ala Ala 
385                 390                 395                 

<210> 52
<211> 1200
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<223> Formaldehyde dehydrogenases

<400> 52
atgtctggca atcgtggtgt ggtctatctc ggcccgggca aggtcgaggt gcagaacatt      60

ccctatccga agatgcagga cccgcagggc cggcagatcg accacggggt gatcctgcgg     120

gtggtctcca ccaacatctg cggctccgac cagcacatgg tgcgcggccg caccaccgcg     180

ccggaaggcc tggtgctggg ccacgagatc accggcgagg tggtggagat cgggcgtggc     240

gtggaaacca tgaagatcgg cgacctggtc tcggtaccgt tcaacgtcgc ctgcggccac     300

tgccgtacct gcaaggaaca gcacaccggc gtctgcctga cggtcaatcc ggcgcgtgcc     360

ggcggcgcct acggctacgt cgacatgggt ggctgggtcg gcggccaggc cgaatacgtg     420

ctggtgccct acgccgactt caacctgctg aaactgccca accgcgaagc ggcgatggag     480

aagatccgcg acctgacctg cctttcggac atcctcccca ccggctacca cggcgcggtc     540

accgccggcg tcggcccggg cagcacggtc tacatcgccg gcgccggtcc ggtcggcctg     600

gccgcggcgg cctcggcacg cctgctcggc gcggcggtgg tgatcgtcgg cgacgtcaac     660

ccgacccgcc tggcccacgc caagaaacag ggcttcgaga tcgccgacct gtccaaggac     720

accccgctgc acgaacagat cgccgctctg ctgggcgagc cggaggtcga ctgcgcggtc     780

gatgcggtcg gtttcgaggc gcgcggccac ggccattcgg gctcgcagca ggaagccccg     840

gccaccgtgc tcaactcgct gatgggcatc acccgggtcg ccggcaagat cggcattccc     900

ggcctgtacg tcaccgaaga cccgggcgca gtggacgcgg ccgccaagca cggcgccctg     960

agcatccgct tcggcctggg ctgggccaag tcgcacagct tccataccgg ccagaccccg    1020

gtgatgaagt acaaccgcca gctgatgcag gcgatcatgt gggaccggat caagatcgcc    1080

gacatcgtcg gggtggaagt catcaccctc gacgatgcgc cgaaaggcta tggcgagttc    1140

gacgccgggg tgccgaagaa attcgtcatc gacccacaca acctgttccg cgcggcctga    1200


<210> 53
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> pntAB_MAGE

<400> 53
ttggcgctag atcacaggca taattttcag tacgttatag ggtgtttgtt actaatttat     60

tttaacggag taacatttag ctcg                                            84


<210> 54
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> fdh_MAGE

<400> 54
agttaaacaa aattatttct attaactagt gaattcggtc attgcgtcct gcgcatatta     60

tatgtgaatc acagtgatat gtca                                            84


<210> 55
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> pntA_forward

<400> 55
gccaatctgc aacagtgctc                                                20


<210> 56
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> pntA reverse

<400> 56
tttttggctg gatggcaagc                                                20


<210> 57
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> fdh forward

<400> 57
cgtgacgaat acctgatcgt t                                              21


<210> 58
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> fdh reverse

<400> 58
ggtagcgtta cctttagagt aagagtg                                        27


<210> 59
<211> 474
<212> PRT
<213> Escherichia coli

<220> 
<223> lipoamide dehydrogenase

<400> 59
Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro 
1               5                   10                  15      
Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr 
            20                  25                  30          
Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val 
        35                  40                  45              
Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu 
    50                  55                  60                  
Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys 
65                  70                  75                  80  
Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln 
                85                  90                  95      
Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val 
            100                 105                 110         
Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu 
        115                 120                 125             
Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala 
    130                 135                 140                 
Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro 
145                 150                 155                 160 
Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu 
                165                 170                 175     
Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr 
            180                 185                 190         
Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp 
        195                 200                 205             
Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys 
    210                 215                 220                 
Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala 
225                 230                 235                 240 
Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys 
                245                 250                 255     
Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly 
            260                 265                 270         
Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu 
        275                 280                 285             
Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn 
    290                 295                 300                 
Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu 
305                 310                 315                 320 
Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala 
                325                 330                 335     
Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr 
            340                 345                 350         
Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys 
        355                 360                 365             
Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser 
    370                 375                 380                 
Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile 
385                 390                 395                 400 
Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr 
                405                 410                 415     
Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly 
            420                 425                 430         
Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu 
        435                 440                 445             
His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr 
    450                 455                 460                 
Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys 
465                 470                 

<210> 60
<211> 1425
<212> DNA
<213> Escherichia coli

<220> 
<223> lipoamide dehydrogenase

<400> 60
atgagtactg aaatcaaaac tcaggtcgtg gtacttgggg caggccccgc aggttactcc      60

gctgccttcc gttgcgctga tttaggtctg gaaaccgtaa tcgtagaacg ttacaacacc     120

cttggcggtg tttgcctgaa cgtcggctgt atcccttcta aagcactgct gcacgtagca     180

aaagttatcg aagaagccaa agcgctggct gaacacggta tcgtcttcgg cgaaccgaaa     240

accgatatcg acaagattcg tacctggaaa gagaaagtga tcaatcagct gaccggtggt     300

ctggctggta tggcgaaagg ccgcaaagtc aaagtggtca acggtctggg taaattcacc     360

ggggctaaca ccctggaagt tgaaggtgag aacggcaaaa ccgtgatcaa cttcgacaac     420

gcgatcattg cagcgggttc tcgcccgatc caactgccgt ttattccgca tgaagatccg     480

cgtatctggg actccactga cgcgctggaa ctgaaagaag taccagaacg cctgctggta     540

atgggtggcg gtatcatcgg tctggaaatg ggcaccgttt accacgcgct gggttcacag     600

attgacgtgg ttgaaatgtt cgaccaggtt atcccggcag ctgacaaaga catcgttaaa     660

gtcttcacca agcgtatcag caagaaattc aacctgatgc tggaaaccaa agttaccgcc     720

gttgaagcga aagaagacgg catttatgtg acgatggaag gcaaaaaagc acccgctgaa     780

ccgcagcgtt acgacgccgt gctggtagcg attggtcgtg tgccgaacgg taaaaacctc     840

gacgcaggca aagcaggcgt ggaagttgac gaccgtggtt tcatccgcgt tgacaaacag     900

ctgcgtacca acgtaccgca catctttgct atcggcgata tcgtcggtca accgatgctg     960

gcacacaaag gtgttcacga aggtcacgtt gccgctgaag ttatcgccgg taagaaacac    1020

tacttcgatc cgaaagttat cccgtccatc gcctataccg aaccagaagt tgcatgggtg    1080

ggtctgactg agaaagaagc gaaagagaaa ggcatcagct atgaaaccgc caccttcccg    1140

tgggctgctt ctggtcgtgc tatcgcttcc gactgcgcag acggtatgac caagctgatt    1200

ttcgacaaag aatctcaccg tgtgatcggt ggtgcgattg tcggtactaa cggcggcgag    1260

ctgctgggtg aaatcggcct ggcaatcgaa atgggttgtg atgctgaaga catcgcactg    1320

accatccacg cgcacccgac tctgcacgag tctgtgggcc tggcggcaga agtgttcgaa    1380

ggtagcatta ccgacctgcc gaacccgaaa gcgaagaaga agtaa                    1425


<210> 61
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mutation in the 5'UTR of FDH

<400> 61
atatgcgcag gacgcactga ccgaattcac tagttaatag aaataatttt gtttaacttt     60

aaggaggttt ggaatgcat                                                  79


<210> 62
<211> 78
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mutation in the promoter of pntAB

<400> 62
tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac atgagcagct     60

atgcgaattg gcatacca                                                   78


