﻿                         序列表

<110>  上海交通大学

<120>  一种制备N-乙酰氨基半乳糖转移酶的方法

<160>  19

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1563
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
aagaagaagg atctgcatca tagcaatggt gaagaaaaag cacagagcat ggaaaccctg        60
ccgccgggta aagttcgttg gccggatttt aatcaggaag catacgttgg tggtacaatg       120
gttcgcagcg gccaggaccc ttatgcacgt aataagttta atcaggtgga aagcgataaa       180
ctgcgcatgg atcgtgccat tccggatacc cgccatgatc agtgtcagcg caaacagtgg       240
cgtgtggatc tgccggccac cagtgttgtt attacctttc ataatgaagc ccgtagcgcc       300
ctgctgcgta ccgtggttag cgtgctgaaa aaatctccgc cgcatctgat taaggaaatt       360
attctggttg atgattacag caatgatccg gaagatggtg cactgctggg caaaattgaa       420
aaagttcgcg tgctgcgtaa tgatcgccgt gaaggtctga tgcgcagtcg cgttcgtggt       480
gccgatgcag cccaggcaaa agttctgacc tttctggata gtcattgtga atgtaatgaa       540
cattggctgg aaccgctgct ggaacgtgtt gcagaagatc gcacccgtgt tgttagcccg       600
attattgatg ttattaatat ggataacttc cagtacgttg gtgccagtgc cgatctgaaa       660
ggtggttttg attggaatct ggtttttaaa tgggattata tgaccccgga acagcgtcgt       720
agccgccagg gtaatccggt ggccccgatt aagaccccga tgattgccgg cggcctgttt       780
gttatggata aattttattt cgaggagctg ggcaaatatg atatgatgat ggatgtttgg       840
ggcggcgaaa atctggaaat tagctttcgc gtttggcagt gcggtggtag cctggaaatt       900
attccgtgta gccgcgttgg tcatgttttt cgtaaacagc atccgtatac ctttccgggc       960
ggcagtggca ccgtgtttgc acgtaatacc cgtcgtgccg cagaagtttg gatggatgaa      1020
tataaaaatt tctactacgc ggccgtgccg agtgcacgca atgtgccgta tggtaatatt      1080
cagagccgcc tggaactgcg caaaaaactg agctgtaaac cgtttaaatg gtatctggaa      1140
aatgtttatc cggaactgcg tgttccggat catcaggata ttgcctttgg tgcactgcag      1200
cagggcacca attgtctgga taccctgggc cattttgccg atggcgttgt tggcgtttat      1260
gaatgtcata atgcaggcgg taatcaggaa tgggcactga ccaaagaaaa aagcgtgaaa      1320
cacatggatc tgtgtctgac cgttgttgat cgtgcaccgg gcagcctgat taagctgcag      1380
ggttgccgcg aaaatgatag ccgccagaaa tgggaacaga ttgaaggtaa tagtaaactg      1440
cgccatgttg gcagtaatct gtgtctggat agccgcaccg caaaaagtgg cggcctgagt      1500
gttgaagtgt gtggcccggc cctgagtcag cagtggaaat tcactctgaa tctgcagcag      1560
tag                                                                    1563

<210>  2
<211>  520
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  2
Lys Lys Lys Asp Leu His His Ser Asn Gly Glu Glu Lys Ala Gln Ser 
1               5                   10                  15      
Met Glu Thr Leu Pro Pro Gly Lys Val Arg Trp Pro Asp Phe Asn Gln 
            20                  25                  30          
Glu Ala Tyr Val Gly Gly Thr Met Val Arg Ser Gly Gln Asp Pro Tyr 
        35                  40                  45              
Ala Arg Asn Lys Phe Asn Gln Val Glu Ser Asp Lys Leu Arg Met Asp 
    50                  55                  60                  
Arg Ala Ile Pro Asp Thr Arg His Asp Gln Cys Gln Arg Lys Gln Trp 
65                  70                  75                  80  
Arg Val Asp Leu Pro Ala Thr Ser Val Val Ile Thr Phe His Asn Glu 
                85                  90                  95      
Ala Arg Ser Ala Leu Leu Arg Thr Val Val Ser Val Leu Lys Lys Ser 
            100                 105                 110         
Pro Pro His Leu Ile Lys Glu Ile Ile Leu Val Asp Asp Tyr Ser Asn 
        115                 120                 125             
Asp Pro Glu Asp Gly Ala Leu Leu Gly Lys Ile Glu Lys Val Arg Val 
    130                 135                 140                 
Leu Arg Asn Asp Arg Arg Glu Gly Leu Met Arg Ser Arg Val Arg Gly 
145                 150                 155                 160 
Ala Asp Ala Ala Gln Ala Lys Val Leu Thr Phe Leu Asp Ser His Cys 
                165                 170                 175     
Glu Cys Asn Glu His Trp Leu Glu Pro Leu Leu Glu Arg Val Ala Glu 
            180                 185                 190         
Asp Arg Thr Arg Val Val Ser Pro Ile Ile Asp Val Ile Asn Met Asp 
        195                 200                 205             
Asn Phe Gln Tyr Val Gly Ala Ser Ala Asp Leu Lys Gly Gly Phe Asp 
    210                 215                 220                 
Trp Asn Leu Val Phe Lys Trp Asp Tyr Met Thr Pro Glu Gln Arg Arg 
225                 230                 235                 240 
Ser Arg Gln Gly Asn Pro Val Ala Pro Ile Lys Thr Pro Met Ile Ala 
                245                 250                 255     
Gly Gly Leu Phe Val Met Asp Lys Phe Tyr Phe Glu Glu Leu Gly Lys 
            260                 265                 270         
Tyr Asp Met Met Met Asp Val Trp Gly Gly Glu Asn Leu Glu Ile Ser 
        275                 280                 285             
Phe Arg Val Trp Gln Cys Gly Gly Ser Leu Glu Ile Ile Pro Cys Ser 
    290                 295                 300                 
Arg Val Gly His Val Phe Arg Lys Gln His Pro Tyr Thr Phe Pro Gly 
305                 310                 315                 320 
Gly Ser Gly Thr Val Phe Ala Arg Asn Thr Arg Arg Ala Ala Glu Val 
                325                 330                 335     
Trp Met Asp Glu Tyr Lys Asn Phe Tyr Tyr Ala Ala Val Pro Ser Ala 
            340                 345                 350         
Arg Asn Val Pro Tyr Gly Asn Ile Gln Ser Arg Leu Glu Leu Arg Lys 
        355                 360                 365             
Lys Leu Ser Cys Lys Pro Phe Lys Trp Tyr Leu Glu Asn Val Tyr Pro 
    370                 375                 380                 
Glu Leu Arg Val Pro Asp His Gln Asp Ile Ala Phe Gly Ala Leu Gln 
385                 390                 395                 400 
Gln Gly Thr Asn Cys Leu Asp Thr Leu Gly His Phe Ala Asp Gly Val 
                405                 410                 415     
Val Gly Val Tyr Glu Cys His Asn Ala Gly Gly Asn Gln Glu Trp Ala 
            420                 425                 430         
Leu Thr Lys Glu Lys Ser Val Lys His Met Asp Leu Cys Leu Thr Val 
        435                 440                 445             
Val Asp Arg Ala Pro Gly Ser Leu Ile Lys Leu Gln Gly Cys Arg Glu 
    450                 455                 460                 
Asn Asp Ser Arg Gln Lys Trp Glu Gln Ile Glu Gly Asn Ser Lys Leu 
465                 470                 475                 480 
Arg His Val Gly Ser Asn Leu Cys Leu Asp Ser Arg Thr Ala Lys Ser 
                485                 490                 495     
Gly Gly Leu Ser Val Glu Val Cys Gly Pro Ala Leu Ser Gln Gln Trp 
            500                 505                 510         
Lys Phe Thr Leu Asn Leu Gln Gln 
        515                 520 

<210>  3
<211>  1479
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
atggatgcac cggaagaaga agatcatgtg ctggtgctgc gtaaaagcaa ttttgcagaa        60
gcactggcag cacataaata tctgctggtt gaattttatg caccgtggtg cggtcattgt       120
aaagcactgg caccggaata tgcaaaagca gcaggtaaac tgaaagcaga aggtagcgaa       180
attcgcctgg ccaaagtgga tgccaccgaa gaaagcgatc tggcacagca gtatggcgtg       240
cgtggctatc cgaccattaa gtttttccgt aatggtgaca ccgcaagccc gaaagaatat       300
accgccggtc gtgaagcaga tgatattgtt aattggctga aaaaacgtac cggtccggcc       360
gccaccaccc tgcctgatgg tgcagcagca gaaagcctgg tggaaagtag tgaagttgca       420
gtgattggct ttttcaaaga tgttgaaagt gatagcgcaa aacagtttct gcaggcagcc       480
gaagccattg atgatattcc gtttggcatt accagtaata gcgatgtgtt tagcaaatat       540
cagctggata aagatggtgt ggtgctgttt aaaaaatttg atgaaggccg taataacttc       600
gaaggtgaag ttaccaaaga aaatctgctg gattttatta agcacaatca gctgccgctg       660
gttattgagt ttactgaaca gaccgcaccg aaaatttttg gtggcgaaat taagacccat       720
attctgctgt ttctgccgaa aagcgttagc gattatgatg gtaaactgag caattttaag       780
accgcagcag aatcattcaa aggtaaaatt ctgtttatct tcatcgacag tgatcatacc       840
gataatcagc gtattctgga atttttcggc ctgaaaaaag aagaatgccc ggccgttcgc       900
ctgattaccc tggaagaaga aatgaccaaa tataaaccgg aaagtgaaga actgaccgca       960
gaacgcatta ccgaattttg ccatcgtttt ctggaaggta aaattaagcc gcatctgatg      1020
agccaggaac tgccggaaga ttgggataaa cagccggtga aagttctggt gggtaaaaat      1080
tttgaagatg tggcctttga tgaaaagaaa aatgtttttg tggagttcta cgcaccgtgg      1140
tgtggtcatt gcaaacagct ggccccgatt tgggataaac tgggtgaaac ctataaagat      1200
catgaaaata tcgtgatcgc aaaaatggat agtaccgcaa atgaagttga agccgttaaa      1260
gttcatagtt ttccgaccct gaaatttttc ccggccagcg ccgatcgtac cgtgattgat      1320
tataatggtg aacgcaccct ggatggcttt aaaaaattcc tggaaagtgg tggtcaggat      1380
ggtgcaggcg atgatgatga tctggaagat ctggaagaag ccgaagaacc ggatatggaa      1440
gaagatgatg atcagaaagc cgttaaagat gaactgtaa                             1479

<210>  4
<211>  492
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  4
Met Asp Ala Pro Glu Glu Glu Asp His Val Leu Val Leu Arg Lys Ser 
1               5                   10                  15      
Asn Phe Ala Glu Ala Leu Ala Ala His Lys Tyr Leu Leu Val Glu Phe 
            20                  25                  30          
Tyr Ala Pro Trp Cys Gly His Cys Lys Ala Leu Ala Pro Glu Tyr Ala 
        35                  40                  45              
Lys Ala Ala Gly Lys Leu Lys Ala Glu Gly Ser Glu Ile Arg Leu Ala 
    50                  55                  60                  
Lys Val Asp Ala Thr Glu Glu Ser Asp Leu Ala Gln Gln Tyr Gly Val 
65                  70                  75                  80  
Arg Gly Tyr Pro Thr Ile Lys Phe Phe Arg Asn Gly Asp Thr Ala Ser 
                85                  90                  95      
Pro Lys Glu Tyr Thr Ala Gly Arg Glu Ala Asp Asp Ile Val Asn Trp 
            100                 105                 110         
Leu Lys Lys Arg Thr Gly Pro Ala Ala Thr Thr Leu Pro Asp Gly Ala 
        115                 120                 125             
Ala Ala Glu Ser Leu Val Glu Ser Ser Glu Val Ala Val Ile Gly Phe 
    130                 135                 140                 
Phe Lys Asp Val Glu Ser Asp Ser Ala Lys Gln Phe Leu Gln Ala Ala 
145                 150                 155                 160 
Glu Ala Ile Asp Asp Ile Pro Phe Gly Ile Thr Ser Asn Ser Asp Val 
                165                 170                 175     
Phe Ser Lys Tyr Gln Leu Asp Lys Asp Gly Val Val Leu Phe Lys Lys 
            180                 185                 190         
Phe Asp Glu Gly Arg Asn Asn Phe Glu Gly Glu Val Thr Lys Glu Asn 
        195                 200                 205             
Leu Leu Asp Phe Ile Lys His Asn Gln Leu Pro Leu Val Ile Glu Phe 
    210                 215                 220                 
Thr Glu Gln Thr Ala Pro Lys Ile Phe Gly Gly Glu Ile Lys Thr His 
225                 230                 235                 240 
Ile Leu Leu Phe Leu Pro Lys Ser Val Ser Asp Tyr Asp Gly Lys Leu 
                245                 250                 255     
Ser Asn Phe Lys Thr Ala Ala Glu Ser Phe Lys Gly Lys Ile Leu Phe 
            260                 265                 270         
Ile Phe Ile Asp Ser Asp His Thr Asp Asn Gln Arg Ile Leu Glu Phe 
        275                 280                 285             
Phe Gly Leu Lys Lys Glu Glu Cys Pro Ala Val Arg Leu Ile Thr Leu 
    290                 295                 300                 
Glu Glu Glu Met Thr Lys Tyr Lys Pro Glu Ser Glu Glu Leu Thr Ala 
305                 310                 315                 320 
Glu Arg Ile Thr Glu Phe Cys His Arg Phe Leu Glu Gly Lys Ile Lys 
                325                 330                 335     
Pro His Leu Met Ser Gln Glu Leu Pro Glu Asp Trp Asp Lys Gln Pro 
            340                 345                 350         
Val Lys Val Leu Val Gly Lys Asn Phe Glu Asp Val Ala Phe Asp Glu 
        355                 360                 365             
Lys Lys Asn Val Phe Val Glu Phe Tyr Ala Pro Trp Cys Gly His Cys 
    370                 375                 380                 
Lys Gln Leu Ala Pro Ile Trp Asp Lys Leu Gly Glu Thr Tyr Lys Asp 
385                 390                 395                 400 
His Glu Asn Ile Val Ile Ala Lys Met Asp Ser Thr Ala Asn Glu Val 
                405                 410                 415     
Glu Ala Val Lys Val His Ser Phe Pro Thr Leu Lys Phe Phe Pro Ala 
            420                 425                 430         
Ser Ala Asp Arg Thr Val Ile Asp Tyr Asn Gly Glu Arg Thr Leu Asp 
        435                 440                 445             
Gly Phe Lys Lys Phe Leu Glu Ser Gly Gly Gln Asp Gly Ala Gly Asp 
    450                 455                 460                 
Asp Asp Asp Leu Glu Asp Leu Glu Glu Ala Glu Glu Pro Asp Met Glu 
465                 470                 475                 480 
Glu Asp Asp Asp Gln Lys Ala Val Lys Asp Glu Leu 
                485                 490         

<210>  5
<211>  345
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
atgttttgta cattttttga aaaacatcac cggaagtggg acatactgtt agaaaaaagc        60
acgggtgtga tggaagctat gaaagtgacg agtgaggaaa aggaacagct gagcacagca       120
atcgaccgaa tgaatgaagg actggacgcg tttatccagc tgtataatga atcggaaatt       180
gatgaaccgc ttattcagct tgatgatgat acagccgagt taatgaagca ggcccgagat       240
atgtacggcc aggaaaagct aaatgagaaa ttaaatacaa ttattaaaca gattttatcc       300
atctcagtat ctgaagaagg agaaaaagaa gacgacgacg acaag                       345

<210>  6
<211>  115
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  6
Met Phe Cys Thr Phe Phe Glu Lys His His Arg Lys Trp Asp Ile Leu 
1               5                   10                  15      
Leu Glu Lys Ser Thr Gly Val Met Glu Ala Met Lys Val Thr Ser Glu 
            20                  25                  30          
Glu Lys Glu Gln Leu Ser Thr Ala Ile Asp Arg Met Asn Glu Gly Leu 
        35                  40                  45              
Asp Ala Phe Ile Gln Leu Tyr Asn Glu Ser Glu Ile Asp Glu Pro Leu 
    50                  55                  60                  
Ile Gln Leu Asp Asp Asp Thr Ala Glu Leu Met Lys Gln Ala Arg Asp 
65                  70                  75                  80  
Met Tyr Gly Gln Glu Lys Leu Asn Glu Lys Leu Asn Thr Ile Ile Lys 
                85                  90                  95      
Gln Ile Leu Ser Ile Ser Val Ser Glu Glu Gly Glu Lys Glu Asp Asp 
            100                 105                 110         
Asp Asp Lys 
        115 

<210>  7
<211>  25
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
cgcggatcca tgcgtcgccg tagtc                                              25

<210>  8
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
ggctggtcga cctactgctg                                                    20

<210>  9
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
cgcggatcca agaagaagga                                                    20

<210>  10
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
ggctggtcga cctactgctg                                                    20

<210>  11
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
aaagatatcg atggatgcac c                                                  21

<210>  12
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
cggggtacct tacagttcat c                                                  21

<210>  13
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
cgcggatcca tgttttgtac                                                    20

<210>  14
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
ggctggtcga cctgctgttc                                                    20

<210>  15
<211>  13
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  15
Pro Thr Thr Asp Ser Thr Thr Pro Ala Pro Thr Thr Lys 
1               5                   10              

<210>  16
<211>  13
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  16
Ser Ala Pro Thr Thr Ser Thr Thr Ser Ala Pro Thr Lys 
1               5                   10              

<210>  17
<211>  16
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  17
Ala Met Ser Gln Ser Leu Leu Lys Thr Thr Gln Glu Pro Leu Ala Lys 
1               5                   10                  15      

<210>  18
<211>  15
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  18
Arg Val Pro Thr Thr Ala Ala Ser Thr Pro Asp Ala Val Asp Lys 
1               5                   10                  15  

<210>  19
<211>  393
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  19
Met Glu Glu Pro Gln Ser Asp Pro Ser Val Glu Pro Pro Leu Ser Gln 
1               5                   10                  15      
Glu Thr Phe Ser Asp Leu Trp Lys Leu Leu Pro Glu Asn Asn Val Leu 
            20                  25                  30          
Ser Pro Leu Pro Ser Gln Ala Met Asp Asp Leu Met Leu Ser Pro Asp 
        35                  40                  45              
Asp Ile Glu Gln Trp Phe Thr Glu Asp Pro Gly Pro Asp Glu Ala Pro 
    50                  55                  60                  
Arg Met Pro Glu Ala Ala Pro Pro Val Ala Pro Ala Pro Ala Ala Pro 
65                  70                  75                  80  
Thr Pro Ala Ala Pro Ala Pro Ala Pro Ser Trp Pro Leu Ser Ser Ser 
                85                  90                  95      
Val Pro Ser Gln Lys Thr Tyr Gln Gly Ser Tyr Gly Phe Arg Leu Gly 
            100                 105                 110         
Phe Leu His Ser Gly Thr Ala Lys Ser Val Thr Cys Thr Tyr Ser Pro 
        115                 120                 125             
Ala Leu Asn Lys Met Phe Cys Gln Leu Ala Lys Thr Cys Pro Val Gln 
    130                 135                 140                 
Leu Trp Val Asp Ser Thr Pro Pro Pro Gly Thr Arg Val Arg Ala Met 
145                 150                 155                 160 
Ala Ile Tyr Lys Gln Ser Gln His Met Thr Glu Val Val Arg Arg Cys 
                165                 170                 175     
Pro His His Glu Arg Cys Ser Asp Ser Asp Gly Leu Ala Pro Pro Gln 
            180                 185                 190         
His Leu Ile Arg Val Glu Gly Asn Leu Arg Val Glu Tyr Leu Asp Asp 
        195                 200                 205             
Arg Asn Thr Phe Arg His Ser Val Val Val Pro Tyr Glu Pro Pro Glu 
    210                 215                 220                 
Val Gly Ser Asp Cys Thr Thr Ile His Tyr Asn Tyr Met Cys Asn Ser 
225                 230                 235                 240 
Ser Cys Met Gly Gly Met Asn Arg Arg Pro Ile Leu Thr Ile Ile Thr 
                245                 250                 255     
Leu Glu Asp Ser Ser Gly Asn Leu Leu Gly Arg Asn Ser Phe Glu Val 
            260                 265                 270         
Arg Val Cys Ala Cys Pro Gly Arg Asp Arg Arg Thr Glu Glu Glu Asn 
        275                 280                 285             
Leu Arg Lys Lys Gly Glu Pro His His Glu Leu Pro Pro Gly Ser Thr 
    290                 295                 300                 
Lys Arg Ala Leu Pro Asn Asn Thr Ser Ser Ser Pro Gln Pro Lys Lys 
305                 310                 315                 320 
Lys Pro Leu Asp Gly Glu Tyr Phe Thr Leu Gln Ile Arg Gly Arg Glu 
                325                 330                 335     
Arg Phe Glu Met Phe Arg Glu Leu Asn Glu Ala Leu Glu Leu Lys Asp 
            340                 345                 350         
Ala Gln Ala Gly Lys Glu Pro Gly Gly Ser Arg Ala His Ser Ser His 
        355                 360                 365             
Leu Lys Ser Lys Lys Gly Gln Ser Thr Ser Arg His Lys Lys Leu Met 
    370                 375                 380                 
Phe Lys Thr Glu Gly Pro Asp Ser Asp 
385                 390             

