
                                SEQUENCE LISTING

<110> Juno Therapeutics, Inc.

<120> ANTI-IDIOTYPIC ANTIBODIES TO
  GPRC5D-TARGETED BINDING DOMAINS AND RELATED COMPOSITIONS AND
  METHODS

<130> 735042022640

<140> Not Yet Assigned    
<141> Concurrently Herewith

<150> US 63/945,064       
<151> 2019-12-06  

<150> US 63/061,757       
<151> 2020-08-05  

<160> 45

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H1 (Kabat)

<400> 1
Ser His Ser Met Asn
 1               5  


<210> 2
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H2 (Kabat)

<400> 2
Ser Ile Ser Ser Asp Ser Thr Tyr Thr Tyr Tyr Ala Asp Ser Val Lys
 1               5                  10                  15      
Gly
    


<210> 3
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H3 (Kabat)

<400> 3
Ser Gly Gly Gln Trp Lys Tyr Tyr Asp Tyr
 1               5                  10  


<210> 4
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L1 (Kabat)

<400> 4
Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser
 1               5                  10      


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L2 (Kabat)

<400> 5
Gly Lys Asn Asn Arg Pro Ser
 1               5          


<210> 6
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L3 (Kabat)

<400> 6
Asn Ser Arg Asp Ser Ser Gly Asn Pro Pro Val Val
 1               5                  10          


<210> 7
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-GPRC5D VH chain

<400> 7
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Ser His
            20                  25                  30          
Ser Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser Ser Ile Ser Ser Asp Ser Thr Tyr Thr Tyr Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Ser Gly Gly Gln Trp Lys Tyr Tyr Asp Tyr Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser
        115                 


<210> 8
<211> 110
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-GPRC5D VL chain

<400> 8
Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln
 1               5                  10                  15      
Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala
            20                  25                  30          
Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr
        35                  40                  45              
Gly Lys Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser
    50                  55                  60                  
Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu
65                  70                  75                  80  
Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn Pro
                85                  90                  95      
Pro Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly
            100                 105                 110 


<210> 9
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-GPRC5D scFv (VL-VH)

<400> 9
Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln
 1               5                  10                  15      
Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala
            20                  25                  30          
Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr
        35                  40                  45              
Gly Lys Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser
    50                  55                  60                  
Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu
65                  70                  75                  80  
Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn Pro
                85                  90                  95      
Pro Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ser Arg
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu
        115                 120                 125             
Glu Met Ala Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His
    130                 135                 140                 
Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe
145                 150                 155                 160 
Arg Ser His Ser Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                165                 170                 175     
Glu Trp Val Ser Ser Ile Ser Ser Asp Ser Thr Tyr Thr Tyr Tyr Ala
            180                 185                 190         
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn
        195                 200                 205             
Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
    210                 215                 220                 
Tyr Tyr Cys Ala Arg Ser Gly Gly Gln Trp Lys Tyr Tyr Asp Tyr Trp
225                 230                 235                 240 
Gly Gln Gly Thr Leu Val Thr Val Ser Ser
                245                 250 


<210> 10
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker

<400> 10
Ser Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
 1               5                  10                  15      
Ser Leu Glu Met Ala
            20      


<210> 11
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Spacer

<400> 11
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10          


<210> 12
<211> 28
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 transmembrane domain

<400> 12
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser
 1               5                  10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25              


<210> 13
<211> 42
<212> PRT
<213> Artificial Sequence

<220> 
<223> 4-1BB-derived intracellular co-signaling sequence

<400> 13
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 14
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD3-zeta derived intracellular

<400> 14
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 15
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H1 (Kabat)

<400> 15
Asp Tyr Tyr Met Tyr
 1               5  


<210> 16
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H2 (Kabat)

<400> 16
Thr Ile Ser Asp Gly Gly Ser Tyr Thr Tyr Tyr Pro Asp Ser Val Lys
 1               5                  10                  15      
Gly
    


<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H3 (Kabat, Chothia, AbM)

<400> 17
Asp Trp Asn Pro Tyr Tyr Gly Ser Gly Gly Gly Trp Phe Gly Tyr
 1               5                  10                  15  


<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H1 (Chothia)

<400> 18
Gly Phe Thr Phe Ser Asp Tyr
 1               5          


<210> 19
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H2 (Chothia)

<400> 19
Ser Asp Gly Gly Ser Tyr
 1               5      


<210> 20
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H1 (AbM)

<400> 20
Gly Phe Thr Phe Ser Asp Tyr Tyr Met Tyr
 1               5                  10  


<210> 21
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-H2 (AbM)

<400> 21
Thr Ile Ser Asp Gly Gly Ser Tyr Thr Tyr
 1               5                  10  


<210> 22
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L1 (Kabat, Chothia, AbM)

<400> 22
Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Met Asn
 1               5                  10                  15  


<210> 23
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L2 (Kabat, Chothia, AbM)

<400> 23
Ala Ala Ser Asn Leu Glu Ser
 1               5          


<210> 24
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDR-L3 (Kabat, Chothia, AbM)

<400> 24
Gln Gln Ser Asn Glu Asp Pro Phe Thr
 1               5                  


<210> 25
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-ID VH chain

<400> 25
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
 1               5                  10                  15      
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr
            20                  25                  30          
Tyr Met Tyr Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Asp Trp Val
        35                  40                  45              
Ala Thr Ile Ser Asp Gly Gly Ser Tyr Thr Tyr Tyr Pro Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu Tyr
65                  70                  75                  80  
Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys
                85                  90                  95      
Ala Arg Asp Trp Asn Pro Tyr Tyr Gly Ser Gly Gly Gly Trp Phe Gly
            100                 105                 110         
Tyr Trp Gly Gln Gly Thr Leu Val Thr Ala Ser Ala
        115                 120                 


<210> 26
<211> 111
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-ID VL chain

<400> 26
Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly
 1               5                  10                  15      
Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp
            20                  25                  30          
Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro
        35                  40                  45              
Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His
65                  70                  75                  80  
Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn
                85                  90                  95      
Glu Asp Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110     


<210> 27
<211> 454
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-ID Heavy chain

<400> 27
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
 1               5                  10                  15      
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr
            20                  25                  30          
Tyr Met Tyr Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Asp Trp Val
        35                  40                  45              
Ala Thr Ile Ser Asp Gly Gly Ser Tyr Thr Tyr Tyr Pro Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu Tyr
65                  70                  75                  80  
Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys
                85                  90                  95      
Ala Arg Asp Trp Asn Pro Tyr Tyr Gly Ser Gly Gly Gly Trp Phe Gly
            100                 105                 110         
Tyr Trp Gly Gln Gly Thr Leu Val Thr Ala Ser Ala Ala Lys Thr Thr
        115                 120                 125             
Ala Pro Ser Val Tyr Pro Leu Ala Pro Val Cys Gly Asp Thr Thr Gly
    130                 135                 140                 
Ser Ser Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro
145                 150                 155                 160 
Val Thr Leu Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr
                165                 170                 175     
Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val
            180                 185                 190         
Thr Val Thr Ser Ser Thr Trp Pro Ser Gln Ser Ile Thr Cys Asn Val
        195                 200                 205             
Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Glu Pro Arg
    210                 215                 220                 
Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn
225                 230                 235                 240 
Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp
                245                 250                 255     
Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp
            260                 265                 270         
Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn
        275                 280                 285             
Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn
    290                 295                 300                 
Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp
305                 310                 315                 320 
Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro
                325                 330                 335     
Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala
            340                 345                 350         
Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys
        355                 360                 365             
Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile
    370                 375                 380                 
Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn
385                 390                 395                 400 
Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys
                405                 410                 415     
Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys
            420                 425                 430         
Ser Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe
        435                 440                 445             
Ser Arg Thr Pro Gly Lys
    450                 


<210> 28
<211> 218
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-ID Light chain

<400> 28
Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly
 1               5                  10                  15      
Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp
            20                  25                  30          
Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro
        35                  40                  45              
Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His
65                  70                  75                  80  
Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn
                85                  90                  95      
Glu Asp Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Arg
            100                 105                 110         
Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln
        115                 120                 125             
Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr
    130                 135                 140                 
Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln
145                 150                 155                 160 
Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr
                165                 170                 175     
Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg
            180                 185                 190         
His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro
        195                 200                 205             
Ile Val Lys Ser Phe Asn Arg Asn Glu Cys
    210                 215             


<210> 29
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-ID VH chain

<400> 29
gaagtgcagc tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc 60
tcctgtgcag cctctggatt cactttcagt gactattaca tgtattgggt tcgccagact 120
ccggaaaaga ggctggactg ggtcgcaacc attagtgatg gtggtagtta cacctactat 180
ccagacagtg tgaaggggcg attcaccatc tccagagaca atgccaagaa caacctgtac 240
ctgcaaatga gcagtctgaa gtctgaggac acagccatgt attactgtgc aagagattgg 300
aacccctact acggtagtgg ggggggctgg tttggttact ggggccaagg gactctggtc 360
actgcctctg ca                                                     372

<210> 30
<211> 334
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-ID VL chain

<400> 30
gacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc 60
atctcctgca aggccagcca aagtgttgat tatgatggtg atagttatat gaactggtac 120
caacagaaac caggacagcc acccaaactc ctcatctatg ctgcatccaa tctagaatct 180
gggatcccag ccaggtttag tggcagtggg tctgggacag acttcaccct caacatccat 240
cctgtggagg aggaggatgc tgcaacctat tactgtcagc aaagtaatga ggatccattc 300
acgttcggct cggggacaaa gttggaaata aaac                             334

<210> 31
<211> 1362
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-ID Heavy chain

<400> 31
gaagtgcagc tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc 60
tcctgtgcag cctctggatt cactttcagt gactattaca tgtattgggt tcgccagact 120
ccggaaaaga ggctggactg ggtcgcaacc attagtgatg gtggtagtta cacctactat 180
ccagacagtg tgaaggggcg attcaccatc tccagagaca atgccaagaa caacctgtac 240
ctgcaaatga gcagtctgaa gtctgaggac acagccatgt attactgtgc aagagattgg 300
aacccctact acggtagtgg ggggggctgg tttggttact ggggccaagg gactctggtc 360
actgcctctg cagccaaaac aacagcccca tcggtctatc cactggcccc tgtgtgtgga 420
gatacaactg gctcctcggt gactctagga tgcctggtca agggttattt ccctgagcca 480
gtgaccttga cctggaactc tggatccctg tccagtggtg tgcacacctt cccagctgtc 540
ctgcagtctg acctctacac cctcagcagc tcagtgactg taacctcgag cacctggccc 600
agccagtcca tcacctgcaa tgtggcccac ccggcaagca gcaccaaggt ggacaagaaa 660
attgagccca gagggcccac aatcaagccc tgtcctccat gcaaatgccc agcacctaac 720
ctcttgggtg gaccatccgt cttcatcttc cctccaaaga tcaaggatgt actcatgatc 780
tccctgagcc ccatagtcac atgtgtggtg gtggatgtga gcgaggatga cccagatgtc 840
cagatcagct ggtttgtgaa caacgtggaa gtacacacag ctcagacaca aacccataga 900
gaggattaca acagtactct ccgggtggtc agtgccctcc ccatccagca ccaggactgg 960
atgagtggca aggagttcaa atgcaaggtc aacaacaaag acctcccagc gcccatcgag 1020
agaaccatct caaaacccaa agggtcagta agagctccac aggtatatgt cttgcctcca 1080
ccagaagaag agatgactaa gaaacaggtc actctgacct gcatggtcac agacttcatg 1140
cctgaagaca tttacgtgga gtggaccaac aacgggaaaa cagagctaaa ctacaagaac 1200
actgaaccag tcctggactc tgatggttct tacttcatgt acagcaagct gagagtggaa 1260
aagaagaact gggtggaaag aaatagctac tcctgttcag tggtccacga gggtctgcac 1320
aatcaccaca cgactaagag cttctcccgg actccgggta aa                    1362

<210> 32
<211> 654
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-ID Light chain

<400> 32
gacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc 60
atctcctgca aggccagcca aagtgttgat tatgatggtg atagttatat gaactggtac 120
caacagaaac caggacagcc acccaaactc ctcatctatg ctgcatccaa tctagaatct 180
gggatcccag ccaggtttag tggcagtggg tctgggacag acttcaccct caacatccat 240
cctgtggagg aggaggatgc tgcaacctat tactgtcagc aaagtaatga ggatccattc 300
acgttcggct cggggacaaa gttggaaata aaacgggctg atgctgcacc aactgtatcc 360
atcttcccac catccagtga gcagttaaca tctggaggtg cctcagtcgt gtgcttcttg 420
aacaacttct accccaaaga catcaatgtc aagtggaaga ttgatggcag tgaacgacaa 480
aatggcgtcc tgaacagttg gactgatcag gacagcaaag acagcaccta cagcatgagc 540
agcaccctca cgttgaccaa ggacgagtat gaacgacata acagctatac ctgtgaggcc 600
actcacaaga catcaacttc acccattgtc aagagcttca acaggaatga gtgt       654

<210> 33
<211> 357
<212> PRT
<213> Artificial Sequence

<220> 
<223> Truncated EGFR (tEGFR)

<400> 33
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly
            20                  25                  30          
Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe
        35                  40                  45              
Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala
    50                  55                  60                  
Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu
65                  70                  75                  80  
Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile
                85                  90                  95      
Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu
            100                 105                 110         
Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala
        115                 120                 125             
Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu
    130                 135                 140                 
Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr
145                 150                 155                 160 
Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys
                165                 170                 175     
Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly
            180                 185                 190         
Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu
        195                 200                 205             
Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys
    210                 215                 220                 
Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu
225                 230                 235                 240 
Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met
                245                 250                 255     
Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala
            260                 265                 270         
His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val
        275                 280                 285             
Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His
    290                 295                 300                 
Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro
305                 310                 315                 320 
Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala
                325                 330                 335     
Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly
            340                 345                 350         
Ile Gly Leu Phe Met
        355         


<210> 34
<211> 41
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28 (amino acids 180-220 of P10747)

<400> 34
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 35
<211> 41
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28 (LL to GG)

<400> 35
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 36
<211> 119
<212> PRT
<213> Homo sapiens

<220> 
<223> Hinge-CH3 spacer

<400> 36
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg
 1               5                  10                  15      
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            20                  25                  30          
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        35                  40                  45              
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    50                  55                  60                  
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
65                  70                  75                  80  
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                85                  90                  95      
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            100                 105                 110         
Leu Ser Leu Ser Leu Gly Lys
        115                 


<210> 37
<211> 229
<212> PRT
<213> Homo sapiens

<220> 
<223> Hinge-CH2-CH3 spacer

<400> 37
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220                 
Leu Ser Leu Gly Lys
225                 


<210> 38
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A peptide

<400> 38
Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp
 1               5                  10                  15      
Val Glu Glu Asn Pro Gly Pro Arg
            20                  


<210> 39
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> P2A peptide

<400> 39
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn
 1               5                  10                  15      
Pro Gly Pro
            


<210> 40
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> P2A peptide

<400> 40
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val
 1               5                  10                  15      
Glu Glu Asn Pro Gly Pro
            20          


<210> 41
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> E2A peptide

<400> 41
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser
 1               5                  10                  15      
Asn Pro Gly Pro
            20  


<210> 42
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> E2A peptide

<400> 42
Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp
 1               5                  10                  15      
Val Glu Ser Asn Pro Gly Pro
            20              


<210> 43
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> F2A peptide

<400> 43
Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val
 1               5                  10                  15      
Glu Ser Asn Pro Gly Pro
            20          


<210> 44
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> F2A peptide

<400> 44
Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala
 1               5                  10                  15      
Gly Asp Val Glu Ser Asn Pro Gly Pro
            20                  25  


<210> 45
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A peptide

<400> 45
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro
 1               5                  10                  15      
Gly Pro
        

