                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  Methods and Compositions for Modifying Plant Immunity

<130>  034044.213WO1

<150>  US 62/943,404
<151>  2019-12-04

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  961
<212>  PRT
<213>  Triticum aestivum

<400>  1

Met Asp Ile Val Thr Gly Ala Ile Ala Lys Leu Ile Pro Lys Leu Gly 
1               5                   10                  15      


Glu Leu Leu Val Gly Glu Tyr Lys Leu His Lys Gly Val Lys Lys Asn 
            20                  25                  30          


Ile Glu Asp Leu Leu Lys Glu Leu Lys Thr Met Asn Ala Ala Leu Ile 
        35                  40                  45              


Lys Ile Gly Glu Val Pro Pro Asp Gln Leu Asp Ser Gln Asp Lys Leu 
    50                  55                  60                  


Trp Ala Asp Glu Val Arg Glu Leu Ser Tyr Val Ile Glu Asp Ala Val 
65                  70                  75                  80  


Asp Lys Phe Leu Val Arg Val His Gly Val Glu Pro Asp Asp Asn Thr 
                85                  90                  95      


Asn Gly Phe Lys Gly Leu Met Lys Arg Thr Thr Lys Leu Leu Lys Lys 
            100                 105                 110         


Val Val Asp Lys His Gly Ile Ala His Ala Ile Lys Asp Ile Lys Lys 
        115                 120                 125             


Glu Leu Gln Glu Val Ala Ala Arg Arg Asp Arg Asn Lys Phe Asp Gly 
    130                 135                 140                 


Ile Ala Ser Ile Pro Thr Glu Ala Ile Asp Pro Arg Leu Arg Ala Leu 
145                 150                 155                 160 


Tyr Ile Glu Ala Ala Glu Leu Val Gly Ile Tyr Gly Lys Arg Asp Gln 
                165                 170                 175     


Glu Leu Met Ser Leu Leu Ser Leu Glu Gly Asp Asp Ala Ser Thr Lys 
            180                 185                 190         


Lys Leu Lys Lys Val Ser Ile Val Gly Phe Gly Gly Leu Gly Lys Thr 
        195                 200                 205             


Thr Leu Ala Lys Ala Val Tyr Glu Lys Ile Lys Gly Asp Phe Asp Cys 
    210                 215                 220                 


His Ala Phe Val Pro Val Gly Gln Asn Pro Asp Lys Lys Lys Val Phe 
225                 230                 235                 240 


Arg Asp Ile Leu Met Asp Leu Ser Asn Ser Asn Ser Asp Leu Ala Leu 
                245                 250                 255     


Leu Asp Glu Arg Gln Leu Ile Asn Lys Leu His Lys Phe Leu Glu Asn 
            260                 265                 270         


Lys Arg Tyr Leu Val Ile Ile Asp Asp Val Trp Asp Glu Gly Leu Trp 
        275                 280                 285             


Lys Asp Ile Asn Leu Ala Phe Ser Asn Arg Asn Asn Leu Gly Ser Arg 
    290                 295                 300                 


Leu Ile Ile Thr Thr Arg Ile Phe Gly Val Ser Glu Ser Cys Cys Ser 
305                 310                 315                 320 


Ser Ala Asp Asp Pro Val Tyr Glu Ile Glu Pro Leu Ser Ile Asp Asp 
                325                 330                 335     


Ser Ser Lys Leu Phe Tyr Thr Arg Ile Phe Ser Asp Ser Gly Cys Pro 
            340                 345                 350         


Lys Glu Phe Glu Gln Val Ser Lys Asp Ile Leu Lys Lys Cys Gly Gly 
        355                 360                 365             


Val Ser Leu Ala Ile Ile Thr Ile Ala Ser Ala Leu Ala Ser Gly Gln 
    370                 375                 380                 


Gln Val Lys Pro Lys His Glu Trp Asp Ile Leu Leu Gln Ser Leu Gly 
385                 390                 395                 400 


Ser Gly Val Thr Lys Asp Asn Ser Leu Val Glu Met Arg Arg Ile Leu 
                405                 410                 415     


Ser Phe Ser Tyr Tyr Asn Leu Pro Ser His Leu Lys Thr Cys Leu Leu 
            420                 425                 430         


Tyr Leu Cys Ile Tyr Pro Glu Asp Ser Met Ile His Arg Asp Arg Leu 
        435                 440                 445             


Ile Trp Lys Trp Val Ala Glu Gly Phe Val His His Gly Asp Gln Gly 
    450                 455                 460                 


Thr Ser Leu Phe Leu Val Gly Leu Asn Tyr Phe Asn Gln Leu Ile Asn 
465                 470                 475                 480 


Arg Ser Met Leu Gln Pro Ile Tyr Ser Asp Met Gly Asn Val Tyr Ala 
                485                 490                 495     


Cys Arg Val His Asp Met Val Leu Asp Leu Ile Cys Asn Leu Ser His 
            500                 505                 510         


Glu Ala Lys Phe Val Asn Val Phe Asp Gly Thr Gly Asn Ile Met Ser 
        515                 520                 525             


Ser Gln Ser Asn Val Arg Arg Leu Ser Leu Gln Asn Lys Asn Glu Asp 
    530                 535                 540                 


His Gln Ala Lys Pro Leu Thr Asn Ile Met Ser Ile Ser Gln Val Arg 
545                 550                 555                 560 


Ser Ile Thr Ile Phe Pro Pro Ala Val Ser Ile Met Pro Ala Leu Ser 
                565                 570                 575     


Arg Phe Glu Val Leu Arg Val Leu Asp Leu Ser Asp Cys Asn Leu Gly 
            580                 585                 590         


Glu Ser Ser Ser Leu Gln Pro Asn Leu Lys Gly Val Gly His Leu Ile 
        595                 600                 605             


His Leu Arg Tyr Leu Gly Leu Ser Gly Thr Arg Ile Ser Lys Leu Pro 
    610                 615                 620                 


Ala Glu Ile Gly Thr Leu Gln Phe Leu Glu Val Leu Asp Leu Gly Tyr 
625                 630                 635                 640 


Asn His Glu Leu Asp Glu Leu Pro Ser Thr Leu Phe Lys Leu Arg Arg 
                645                 650                 655     


Leu Ile Tyr Leu Asn Val Ser Pro Tyr Lys Val Val Pro Thr Pro Gly 
            660                 665                 670         


Val Leu Gln Asn Met Thr Ser Ile Glu Val Leu Arg Gly Ile Phe Val 
        675                 680                 685             


Ser Leu Asn Ile Ile Ala Gln Glu Leu Gly Lys Leu Ala Arg Leu Arg 
    690                 695                 700                 


Glu Leu Gln Ile Tyr Phe Lys Asp Gly Ser Leu Asp Leu Tyr Glu Gly 
705                 710                 715                 720 


Phe Val Lys Ser Leu Cys Asn Leu His His Ile Glu Ser Leu Ile Val 
                725                 730                 735     


Ser Cys Asn Ser Gly Glu Thr Ser Phe Glu Leu Met Asp Leu Leu Gly 
            740                 745                 750         


Glu Gln Trp Val Pro Pro Val His Leu Arg Glu Phe Val Ser Glu Met 
        755                 760                 765             


Pro Ser Gln Leu Ser Ala Leu Arg Gly Trp Ile Lys Arg Asp Pro Ser 
    770                 775                 780                 


His Leu Ser Asn Leu Ser Glu Leu Ile Leu Pro Thr Val Lys Glu Val 
785                 790                 795                 800 


Gln Gln Glu Asp Val Glu Ile Ile Gly Gly Leu Leu Ser Leu Arg Arg 
                805                 810                 815     


Leu Leu Ile Glu Ser Thr His Gln Thr Gln Arg Leu Leu Val Ile Arg 
            820                 825                 830         


Ala Asp Gly Phe Arg Cys Met Val Asp Phe Tyr Leu Asn Cys Gly Ser 
        835                 840                 845             


Ala Thr Gln Ile Met Phe Glu Ser Gly Ala Leu Pro Arg Ala Glu Glu 
    850                 855                 860                 


Val Cys Phe Ser Leu Gly Val Arg Val Ala Lys Glu Asp Gly Asn Arg 
865                 870                 875                 880 


Gly Phe Asp Leu Gly Leu Gln Gly Asn Leu Leu Ser Leu Arg Arg Val 
                885                 890                 895     


Val Trp Val Lys Met Tyr Cys Gly Gly Ala Arg Val Gly Glu Ala Lys 
            900                 905                 910         


Glu Ala Lys Ala Ala Val Arg His Ala Leu Glu Asp His Pro Asn His 
        915                 920                 925             


Pro Pro Ile Gln Ile Asn Met Phe Pro Arg Ile Ala Glu Gly Ala Gln 
    930                 935                 940                 


Asp Asp Asp Leu Met Cys Tyr Pro Val Gly Gly Pro Ile Ser Asp Ala 
945                 950                 955                 960 


Glu 
    


<210>  2
<211>  956
<212>  PRT
<213>  Secale cereale

<400>  2

Met Asn Ile Val Thr Gly Ala Met Gly Ser Leu Ile Pro Lys Leu Gly 
1               5                   10                  15      


Glu Leu Leu Met Asp Glu Tyr Lys Leu His Lys Arg Ile Lys Lys Asp 
            20                  25                  30          


Val Glu Phe Leu Lys Lys Glu Leu Glu Ser Met His Ala Ala Leu Ile 
        35                  40                  45              


Lys Val Gly Glu Val Pro Arg Asp Gln Leu Asp Arg Gln Val Lys Leu 
    50                  55                  60                  


Trp Ala Asp Glu Val Arg Glu Leu Ser Tyr Asn Met Glu Asp Val Val 
65                  70                  75                  80  


Asp Lys Phe Leu Val Arg Val Asp Gly Asp Gly Ile Gln Gln Pro His 
                85                  90                  95      


Asp Asn Ser Gly Arg Phe Lys Glu Leu Lys Asn Lys Met Ile Gly Leu 
            100                 105                 110         


Phe Lys Lys Gly Arg Asn His His Arg Ile Ala Asp Ala Ile Lys Glu 
        115                 120                 125             


Ile Lys Glu Gln Leu Gln Glu Val Ala Ala Arg Arg Asp Arg Asn Lys 
    130                 135                 140                 


Val Ala Val Pro Asn Pro Met Glu Pro Ile Thr Ile Asp Pro Cys Leu 
145                 150                 155                 160 


Arg Ala Leu Tyr Ala Glu Ala Thr Glu Leu Val Gly Ile Tyr Gly Lys 
                165                 170                 175     


Arg Asp Glu Glu Leu Met Arg Leu Leu Ser Met Glu Gly Asp Asp Ala 
            180                 185                 190         


Ser Asn Lys Arg Leu Lys Lys Val Ser Ile Val Gly Phe Gly Gly Leu 
        195                 200                 205             


Gly Lys Thr Thr Leu Ala Arg Ala Val Tyr Asp Lys Ile Lys Gly Asp 
    210                 215                 220                 


Phe Asp Cys Arg Ala Phe Val Pro Val Gly Gln Asn Pro Asp Met Lys 
225                 230                 235                 240 


Lys Val Leu Arg Asp Ile Leu Ile Asp Leu Gly Asn Pro His Ser Asp 
                245                 250                 255     


Leu Ala Ile Leu Asp Asp Lys Gln Leu Val Lys Lys Leu His Asp Phe 
            260                 265                 270         


Leu Glu Asn Lys Arg Tyr Leu Val Ile Ile Asp Asp Ile Trp Asp Glu 
        275                 280                 285             


Met Leu Trp Glu Gly Ile Asn Phe Ala Phe Ser Asn Arg Asn Asn Leu 
    290                 295                 300                 


Gly Ser Arg Leu Ile Thr Thr Thr Arg Asn Phe Asp Val Ser Lys Ser 
305                 310                 315                 320 


Cys Cys Leu Ser Ala Asp Asp Ser Ile Tyr Lys Met Lys Pro Leu Ser 
                325                 330                 335     


Thr Asp Asp Ser Arg Arg Leu Phe His Lys Arg Ile Phe Pro Asp Ala 
            340                 345                 350         


Gly Gly Cys Pro Ser Glu Phe Gln Gln Val Ser Glu Asp Ile Leu Lys 
        355                 360                 365             


Lys Cys Gly Gly Val Pro Leu Ala Ile Ile Thr Ile Ala Ser Ala Leu 
    370                 375                 380                 


Ala Ser Gly Gln His Val Lys Pro Lys His Glu Trp Asp Ile Leu Leu 
385                 390                 395                 400 


Gln Ser Leu Gly Ser Gly Val Thr Lys Asp Asn Ser Leu Val Glu Met 
                405                 410                 415     


Arg Arg Ile Leu Ser Phe Ser Tyr Tyr Asn Leu Pro Ser His Leu Lys 
            420                 425                 430         


Thr Cys Leu Leu Tyr Leu Cys Ile Tyr Pro Glu Asp Ser Thr Ile Gly 
        435                 440                 445             


Arg Asp Arg Leu Ile Trp Lys Trp Val Ala Glu Gly Phe Val His His 
    450                 455                 460                 


Gly Asp Gln Gly Thr Ser Leu Phe Leu Val Gly Leu Asn Tyr Phe Asn 
465                 470                 475                 480 


Gln Leu Ile Asn Arg Ser Met Ile Gln Pro Ile Tyr Asp Glu Leu Gly 
                485                 490                 495     


Gln Val His Ala Cys Arg Val His Asp Met Val Leu Asp Leu Ile Cys 
            500                 505                 510         


Asn Phe Ser His Glu Ala Lys Phe Val Asn Val Leu Asp Gly Thr Gly 
        515                 520                 525             


Asn Ser Ile Ser Ser Gln Ser Asn Val Arg Arg Leu Ser Leu Gln Asn 
    530                 535                 540                 


Lys Met Glu Asp His Gln Ala Lys Pro Leu Thr Asn Ile Met Ser Met 
545                 550                 555                 560 


Ser Arg Val Arg Ser Ile Thr Ile Phe Pro Pro Ala Val Ser Ile Met 
                565                 570                 575     


Pro Ser Leu Ser Met Phe Glu Val Leu Arg Val Leu Asp Leu Ser Asn 
            580                 585                 590         


Cys Asp Leu Gly Lys Ser Ser Ser Leu Gln Leu Asn Leu Lys Gly Val 
        595                 600                 605             


Gly His Leu Ile His Leu Arg Tyr Leu Asp Leu Gln Gly Thr Gln Ile 
    610                 615                 620                 


Ser Glu Leu Pro Thr Glu Ile Gly Asn Leu Gln Phe Leu Glu Val Leu 
625                 630                 635                 640 


Asp Leu Asp Asn Asn Tyr Glu Leu Asp Glu Leu Pro Ser Thr Leu Phe 
                645                 650                 655     


Lys Leu Arg Arg Leu Ile Tyr Leu Asn Val Met Leu Tyr Lys Val Val 
            660                 665                 670         


Pro Thr Pro Gly Val Leu Gln Asn Met Thr Ser Ile Glu Val Leu Arg 
        675                 680                 685             


Gly Val Leu Val Ser Leu Asn Ile Ile Ala Gln Glu Leu Gly Asn Leu 
    690                 695                 700                 


Thr Arg Leu Arg Glu Leu Lys Ile Cys Phe Lys Asp Gly Asn Leu Asp 
705                 710                 715                 720 


Ser Tyr Lys Leu Phe Val Lys Ser Leu Gly Asn Leu His His Ile Glu 
                725                 730                 735     


Ser Leu Ser Ile Ser Tyr Asn Ser Lys Glu Thr Ser Phe Glu Leu Met 
            740                 745                 750         


Asp Leu Leu Gly Glu Arg Trp Val Pro Pro Val His Leu Arg Glu Phe 
        755                 760                 765             


Val Ser Trp Met Pro Ser Gln Leu Ser Ala Leu Arg Gly Trp Ile Lys 
    770                 775                 780                 


Arg Asp Pro Ser His Leu Ser Asn Leu Ser Glu Leu Ile Leu Trp Pro 
785                 790                 795                 800 


Val Lys Glu Val Gln Gln Glu Asp Val Glu Ile Ile Gly Gly Leu Leu 
                805                 810                 815     


Ser Leu Arg Arg Leu Trp Ile Lys Ser Thr His Gln Thr Gln Arg Leu 
            820                 825                 830         


Leu Val Ile Arg Ala Asp Gly Phe Arg Cys Met Met Asp Phe Glu Leu 
        835                 840                 845             


Asn Cys Gly Ser Ala Ala Gln Ile Met Phe Glu Pro Gly Ala Leu Pro 
    850                 855                 860                 


Arg Ala Glu Val Leu Val Phe Ser Leu Gly Val Arg Val Ala Gln Glu 
865                 870                 875                 880 


Asp Gly Asn Cys Gly Phe Asp Leu Gly Leu Gln Gly Asn Leu Leu Ser 
                885                 890                 895     


Leu Arg His Asp Val Phe Val Arg Ile Tyr Cys Gly Gly Ala Arg Val 
            900                 905                 910         


Gly Glu Ala Lys Glu Ala Glu Ala Ala Val Arg His Ala Leu Glu Ala 
        915                 920                 925             


His Pro Asn His Pro Pro Ile Asp Ile Glu Met Thr Pro Tyr Ile Ala 
    930                 935                 940                 


Glu Gly Ala Arg Asp Asp Asp Leu Cys Glu Glu Asn 
945                 950                 955     


<210>  3
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full length AvrSr50 sequence as reported in Chen et al. (2017) 
       Loss of by Somatic Exchange in Stem Rust Leads to Virulence for 
       Resistance in Wheat. Science 358 (6370): 1607-10.

<400>  3

Met Met His Ser Ile Ile Phe Gln Thr Leu Leu Ile Ile Thr Ile Val 
1               5                   10                  15      


Phe Ser Lys Val Trp Gly Ala Arg Ser Leu Val Lys Ile Asp Trp Ser 
            20                  25                  30          


Gly Ser Glu Tyr Thr Ile Leu Gly Ala Asn His Tyr Glu Glu Pro Asn 
        35                  40                  45              


Thr Gly Ala Ala Ala Gln Phe Pro Gly Thr Met Thr Val Asp Asp Gly 
    50                  55                  60                  


Arg Ser Pro Tyr Ile Val Arg Lys Leu Arg Asn Ser Ser Gly Lys Arg 
65                  70                  75                  80  


Phe Tyr Val Phe Thr Gly His Pro Gln Gln Pro Ile Val Trp Asn Pro 
                85                  90                  95      


His Glu Glu Ile Glu Ile Gln Phe Asn Arg Lys Phe Leu Ile Ala Val 
            100                 105                 110         


Leu Thr Glu Phe Glu Ala Asp Ser Gln Val Phe Asn His Phe Ala Arg 
        115                 120                 125             


Arg Gln His Arg 
    130         


<210>  4
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cloned AvrSr50 sequence without signal peptide.

<400>  4

Met Ala Arg Ser Leu Val Lys Ile Asp Trp Ser Gly Ser Glu Tyr Thr 
1               5                   10                  15      


Ile Leu Gly Ala Asn His Tyr Glu Glu Pro Asn Thr Gly Ala Ala Ala 
            20                  25                  30          


Gln Phe Pro Gly Thr Met Thr Val Asp Asp Gly Arg Ser Pro Tyr Ile 
        35                  40                  45              


Val Arg Lys Leu Arg Asn Ser Ser Gly Lys Arg Phe Tyr Val Phe Thr 
    50                  55                  60                  


Gly His Pro Gln Gln Pro Ile Val Trp Asn Pro His Glu Glu Ile Glu 
65                  70                  75                  80  


Ile Gln Phe Asn Arg Lys Phe Leu Ile Ala Val Leu Thr Glu Phe Glu 
                85                  90                  95      


Ala Asp Ser Gln Val Phe Asn His Phe Ala Arg Arg Gln His Arg 
            100                 105                 110     


