                         SEQUENCE LISTING

<110>  Northwestern University
       LanzaTech, Inc.
       Jewett, Michael C.
       Karim, Ashty S.
       Koepke, Michael
       Juminaga, Darmawi
       Liew, Fungmin
 
<120>  MODULAR, CELL-FREE PROTEIN EXPRESSION VECTORS TO ACCELERATE 
       BIOLOGICAL DESIGN IN CELLS

<130>  702581.01878_NU2019-034-02

<150>  US 62/943,036
<151>  2019-12-03

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  2557
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  1
gtcgcgctgg agaccctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg       60

ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc      120

tgaaagccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca      180

tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact      240

caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc      300

caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat      360

caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga      420

cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt      480

tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat      540

tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt      600

cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg      660

tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa      720

attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt      780

tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg      840

cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt      900

tggaatttaa tcgcggcttc gagcaagacg tttcccgttg aatatggctc ataacacccc      960

ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt     1020

gtgcaatgta acatcagaga ttttgagaca caacgtgaga tcaaaggatc ttcttgagat     1080

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     1140

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     1200

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     1260

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     1320

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     1380

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     1440

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     1500

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     1560

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     1620

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcgatc     1680

ccgcgaaatt aatacgactc actataggga caccacaacg gtttccctct agaaataatt     1740

ttgtttaact ttaagaagga gaggtctctt catggaactt aataatgtaa tattggaaaa     1800

agaaggaaaa gtagctgtag taacgattaa tagacccaaa gcattaaatg cattaaattc     1860

agatacttta aaagaaatgg attatgttat aggtgaaata gaaaatgatt cagaagtact     1920

tgcagttata cttacaggtg cgggagagaa aagctttgtt gcaggagctg acatatcgga     1980

aatgaaggaa atgaatacta ttgaaggtag aaaatttggc atactaggta ataaagtgtt     2040

tagaaggttg gaattgcttg aaaagccagt aattgctgca gttaatggat ttgcacttgg     2100

cggcggctgt gagatagcta tgtcttgcga tataagaatc gcatcttcaa atgcaagatt     2160

tggacagcct gaagttggat taggtattac accagggttt ggcggcactc agagattatc     2220

tagattagta ggtatgggaa tggctaagca acttatattt acagcacaaa atataaaggc     2280

agatgaagct ttaagaatag gacttgtaaa taaagtagta gaaccttctg aattaatgaa     2340

tactgcaaaa gaaatagcta ataaaatagt ctctaatgca ccagtggcag ttaaattatc     2400

aaaacaagca ataaatagag gtatgcaatg tgacatagat acggcacttg ctttcgaatc     2460

agaagcattt ggtgaatgct tctctactga agaccaaaaa gatgctatga cagcatttat     2520

tgaaaaacga aagattgaag gattcaaaaa tagataa                              2557


<210>  2
<211>  3789
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  2
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgacg cgttagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc aggcgaggtc tcccgctaat aagaagaagt      360

gtgaaaaagc gcagctgaaa tagctgcgct tttttgtgtc ataaggcgcg ctcactatct      420

gcggaacctg cctccttatc tgataaaaaa tattcgctgc atctttgact tgttattttc      480

tttcaaatgc ctaatggaat tgtgagcgga taacaattaa ttatctttta aaattataac      540

aaatgtgata aaatacaggg gatgaaaaca ttatctaaaa attaaggagg tgtttctaat      600

gagagaccgt cctgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc      660

tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca      720

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc      780

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg      840

cagcctatac gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct      900

gtttgtggat gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct      960

ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat     1020

cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccag tctccgttat     1080

cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct     1140

gatgttctgg ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat     1200

ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta     1260

ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg     1320

gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc     1380

agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt     1440

gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc     1500

gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag     1560

gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg     1620

gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa     1680

tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc     1740

agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc     1800

ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga     1860

tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa     1920

acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct     1980

ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat     2040

gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt     2100

ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta     2160

tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga     2220

ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg     2280

ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct     2340

ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc     2400

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     2460

aataaccctg ataaatgctt caataatagc acgtgaggag ggccaccatg gccaagttga     2520

ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg     2580

accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg     2640

acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct     2700

gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga     2760

acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg     2820

agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact     2880

gacacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     2940

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     3000

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     3060

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     3120

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc     3180

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     3240

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     3300

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     3360

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     3420

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     3480

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     3540

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     3600

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     3660

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg     3720

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg     3780

aagcggaag                                                             3789


<210>  3
<211>  4737
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  3
atggatttta atttaacaag agaacaagaa ttagtaagac agatggttag agaatttgct       60

gaaaatgaag ttaaacctat agcagcagaa attgatgaaa cagaaagatt tccaatggaa      120

aatgtaaaga aaatgggtca gtatggtatg atgggaattc cattttcaaa agagtatggt      180

ggcgcaggtg gagatgtatt atcttatata atcgccgttg aggaattatc aaaggtttgc      240

ggtactacag gagttattct ttcagcacat acatcacttt gtgcttcatt aataaatgaa      300

catggtacag aagaacaaaa acaaaaatat ttagtacctt tagctaaagg tgaaaaaata      360

ggtgcttatg gattgactga gccaaatgca ggaacagatt ctggagcaca acaaacagta      420

gctgtacttg aaggagatca ttatgtaatt aatggttcaa aaatattcat aactaatgga      480

ggagttgcag atacttttgt tatatttgca atgactgaca gaactaaagg aacaaaaggt      540

atatcagcat ttataataga aaaaggcttc aaaggtttct ctattggtaa agttgaacaa      600

aagcttggaa taagagcttc atcaacaact gaacttgtat ttgaagatat gatagtacca      660

gtagaaaaca tgattggtaa agaaggaaaa ggcttcccta tagcaatgaa aactcttgat      720

ggaggaagaa ttggtatagc agctcaagct ttaggtatag ctgaaggtgc tttcaacgaa      780

gcaagagctt acatgaagga gagaaaacaa tttggaagaa gccttgacaa attccaaggt      840

cttgcatgga tgatggcaga tatggatgta gctatagaat cagctagata tttagtatat      900

aaagcagcat atcttaaaca agcaggactt ccatacacag ttgatgctgc aagagctaag      960

cttcatgctg caaatgtagc aatggatgta acaactaagg cagtacaatt atttggtgga     1020

tacggatata caaaagatta tccagttgaa agaatgatga gagatgctaa gataactgaa     1080

atatatgaag gaacttcaga agttcagaaa ttagttattt caggaaaaat ttttagataa     1140

tttaaggagg ttaagaggat gaatatagtt gtttgtttaa aacaagttcc agatacagcg     1200

gaagttagaa tagatccagt taagggaaca cttataagag aaggagttcc atcaataata     1260

aatccagatg ataaaaacgc acttgaggaa gctttagtat taaaagataa ttatggtgca     1320

catgtaacag ttataagtat gggacctcca caagctaaaa atgctttagt agaagctttg     1380

gctatgggtg ctgatgaagc tgtactttta acagatagag catttggagg agcagataca     1440

cttgcgactt cacatacaat tgcagcagga attaagaagc taaaatatga tatagttttt     1500

gctggaaggc aggctataga tggagataca gctcaggttg gaccagaaat agctgagcat     1560

cttggaatac ctcaagtaac ttatgttgag aaagttgaag ttgatggaga tactttaaag     1620

attagaaaag cttgggaaga tggatatgaa gttgttgaag ttaagacacc agttctttta     1680

acagcaatta aagaattaaa tgttccaaga tatatgagtg tagaaaaaat attcggagca     1740

tttgataaag aagtaaaaat gtggactgcc gatgatatag atgtagataa ggctaattta     1800

ggtcttaaag gttcaccaac taaagttaag aagtcatcaa ctaaagaagt taaaggacag     1860

ggagaagtta ttgataagcc tgttaaggaa gcagctgcat atgttgtctc aaaattaaaa     1920

gaagaacact atatttaagt taggagggat ttttcaatga ataaagcaga ttacaagggc     1980

gtatgggtgt ttgctgaaca aagagacgga gaattacaaa aggtatcatt ggaattatta     2040

ggtaaaggta aggaaatggc tgagaaatta ggcgttgaat taacagctgt tttacttgga     2100

cataatactg aaaaaatgtc aaaggattta ttatctcatg gagcagataa ggttttagca     2160

gcagataatg aacttttagc acatttttca acagatggat atgctaaagt tatatgtgat     2220

ttagttaatg aaagaaagcc agaaatatta ttcataggag ctactttcat aggaagagat     2280

ttaggaccaa gaatagcagc aagactttct actggtttaa ctgctgattg tacatcactt     2340

gacatagatg tagaaaatag agatttattg gctacaagac cagcgtttgg tggaaatttg     2400

atagctacaa tagtttgttc agaccacaga ccacaaatgg ctacagtaag acctggtgtg     2460

tttgaaaaat tacctgttaa tgatgcaaat gtttctgatg ataaaataga aaaagttgca     2520

attaaattaa cagcatcaga cataagaaca aaagtttcaa aagttgttaa gcttgctaaa     2580

gatattgcag atatcggaga agctaaggta ttagttgctg gtggtagagg agttggaagc     2640

aaagaaaact ttgaaaaact tgaagagtta gcaagtttac ttggtggaac aatagccgct     2700

tcaagagcag caatagaaaa agaatgggtt gataaggacc ttcaagtagg tcaaactggt     2760

aaaactgtaa gaccaactct ttatattgca tgtggtatat caggagctat ccagcattta     2820

gcaggtatgc aagattcaga ttacataatt gctataaata aagatgtaga agccccaata     2880

atgaaggtag cagatttggc tatagttggt gatgtaaata aagttgtacc agaattaata     2940

gctcaagtta aagctgctaa taattaagtc ggctcggaga ccctgctaac aaagcccgaa     3000

aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct     3060

ctaaacgggt cttgaggggt tttttgctga aagccaattc tgattagaaa aactcatcga     3120

gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa     3180

gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct     3240

ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt     3300

caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg     3360

gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat     3420

caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa     3480

atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga     3540

acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga     3600

atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa     3660

aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat     3720

ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg     3780

gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt     3840

tatacccata taaatcagca tccatgttgg aatttaatcg cggcttcgag caagacgttt     3900

cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta     3960

ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa     4020

cgtgagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     4080

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     4140

tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     4200

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     4260

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     4320

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     4380

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     4440

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     4500

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     4560

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     4620

ctatggaaaa acgccagcaa cgcgatcccg cgaaattaat acgactcact atagggacac     4680

cacaacggtt tccctctaga aataattttg tttaacttta agaaggagag gtctcta        4737


<210>  4
<211>  4202
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  4
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgacg cgttagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc agggaggtct ccgctcaatc atactcgagg      360

cgcgcagcct gaatggcgaa tggcgctagc ataatcaatc gtcccttcgt gtaaacgaag      420

gggcgttttt tatttggcgc gtcgttttac aacggagata gtcataatag ttccagaata      480

gttcaattta gaaattagac taaacttcaa aatgtttgtt aaatatatac caaactagta      540

tagatatttt ttaaatactg gacttaaaca gtagtaattt gcctaaaaaa ttttttcaat      600

tttttttaaa aaatcctttt caagttgtac attgttatgg taatatgtaa ttgaagaagt      660

tatgtagtaa tattgtaaac gtttcttgat ttttttacat ccatgtagtg cttaaaaaac      720

caaaatatgt cacatgcaat tgtatatttc aaataacaat atttattttc tcgttaaatt      780

cacaaataat ttattaataa tatcaataac caagattata cttaaatgga tgtttatttt      840

ttaacacttt tatagtaaat atatttattt tatgtagtaa aaaggttata attataattg      900

tatttattac aattaattaa aataaaaaat agggttttag gtaaaattaa gttattttaa      960

gaagtaatta caataaaaat tgaagttatt tctttaagga gggaattatt cttaagagac     1020

cgtcctgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg     1080

cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt     1140

gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc     1200

agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta     1260

tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     1320

gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt     1380

gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     1440

gaaagctggc gcatgatgac caccgatatg gccagtgtgc cagtctccgt tatcggggaa     1500

gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     1560

tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc     1620

acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct     1680

atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca     1740

tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg     1800

gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca     1860

aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc     1920

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     1980

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     2040

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     2100

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     2160

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     2220

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     2280

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     2340

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     2400

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     2460

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     2520

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     2580

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     2640

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     2700

gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg     2760

catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac     2820

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc     2880

ctgataaatg cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc     2940

cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct     3000

cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac     3060

cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg     3120

ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg     3180

ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc     3240

cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt     3300

gctaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat     3360

gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat     3420

caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa     3480

accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa     3540

ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt     3600

aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt     3660

accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata     3720

gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt     3780

ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac     3840

gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga     3900

gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     3960

ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa     4020

aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat     4080

gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc     4140

tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga     4200

ag                                                                    4202


<210>  5
<211>  2944
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  5
gtgtggagac cctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga       60

gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa      120

agccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc      180

aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc      240

gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac      300

atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc      360

atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg      420

ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt      480

cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca      540

aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc      600

tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag      660

taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc      720

cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc      780

atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc      840

tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga      900

atttaatcgc ggcttcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt      960

attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc     1020

aatgtaacat cagagatttt gagacacaac gtgagatcaa aggatcttct tgagatcctt     1080

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt     1140

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc     1200

agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg     1260

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg     1320

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt     1380

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac     1440

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg     1500

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg     1560

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat     1620

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgatcccgc     1680

gaaattaata cgactcacta tagggacacc acaacggttt ccctctagaa ataattttgt     1740

ttaactttaa gaaggagagg tctcacttat gaaagaagta gtaatagcat cggcagtcag     1800

aacagcaatc ggaagttatg gaaaatcact taaagacgta ccagctgtag atcttggtgc     1860

cacagctatt aaagaggcag taaaaaaggc aggtataaaa ccagaagatg taaatgaagt     1920

tatacttggt aacgttttgc aggctggatt aggacaaaat cctgcaagac aagcttcttt     1980

taaagcagga cttcctgttg aaattcctgc tatgactatc aataaagtct gcggatctgg     2040

tttgagaaca gtatcactgg cagcacaaat aataaaggct ggagatgcag atgtaataat     2100

tgctggcggc atggaaaata tgtctagggc accttatctg gcgaataatg cgcgctgggg     2160

atataggatg ggcaatgcaa aatttgttga tgagatgatc acagatggac tatgggatgc     2220

attcaatgat tatcatatgg gaatcacggc agaaaatata gcagaaaggt ggaatatatc     2280

tagagaagaa caagatgaat ttgcattagc atcacaaaaa aaagcagaag aagccattaa     2340

atctggacag ttcaaggacg aaattgttcc tgttgtaata aagggaagaa aaggtgagac     2400

tgtagtagat acagatgagc atccaagatt tggatcaact atagagggac ttgcaaagct     2460

taagccggca tttaaaaaag atggaactgt tacagctggt aatgcatctg gactaaatga     2520

ttgtgctgct gtactcgtaa taatgagtgc tgaaaaagca aaggaactag gagtaaaacc     2580

tcttgctaaa attgtaagtt atggaagtgc aggagtagat cctgctataa tgggctatgg     2640

tccattttat gcaactaagg cagcaattga aaaagcagga tggactgtag atgaattaga     2700

tcttattgaa tcaaacgaag catttgctgc ccaatcttta gcagttgcca aagatttaaa     2760

atttgatatg aataaggtaa atgtaaatgg cggcgctatt gccctcggac accctattgg     2820

tgcatcaggg gcccgtattc ttgttacact tgttcatgcc atgcaaaaaa gggatgcaaa     2880

gaaaggatta gctactcttt gtataggcgg cggccaggga actgctatat tattagaaaa     2940

ataa                                                                  2944


<210>  6
<211>  6058
<212>  DNA
<213>  Artificial

<220>
<223>  Expression Vector for Clostridia species

<400>  6
tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc       60

cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca      120

ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg      180

aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct      240

acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg      300

acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc      360

ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc      420

ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc      480

taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag      540

agcgacttcg cggagctggt gaagtacatc accgacgagc aaggcaagac cgatcgggcc      600

ccctgcagga taaaaaaatt gtagataaat tttataaaat agttttatct acaatttttt      660

tatcaggaaa cagctatgac cgcggccgct cactatctgc ggaacctgcc tccttatctg      720

ataaaaaata ttcgctgcat ctttgacttg ttattttctt tcaaatgcct aatggaattg      780

tgagcggata acaattaatt atcttttaaa attataacaa atgtgataaa atacagggga      840

tgaaaacatt atctaaaaat taaggaggtg ttactcatag agaccactgg atgtcttccg      900

gccgctaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa      960

gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc     1020

aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat     1080

gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa     1140

gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt     1200

aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag     1260

ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc     1320

tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa     1380

gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa     1440

actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac     1500

atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt     1560

attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta     1620

aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg     1680

caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc     1740

ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggggggg     1800

cgtaaacgcc gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg     1860

ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag     1920

gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa     1980

ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt     2040

cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg     2100

tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa     2160

ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat     2220

tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga     2280

taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat     2340

gaccaccgat atggccagtg tgccggtgtc cgttatcggg gaagaagtgg ctgatctcag     2400

ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc     2460

aggctccctt atacacagcc agtctgcagg tcgacgaaga ctagcatggt ctccgtgtca     2520

tgattgtaac aatttataaa taaaaatcac cttttagagg tggttttttt atttataaat     2580

tagtgtaggc gcgccgccat tatttttttg aacaattgac aattcatttc ttatttttta     2640

ttaagtgata gtcaaaaggc ataacagtgc tgaatagaaa gaaatttaca gaaaagaaaa     2700

ttatagaatt tagtatgatt aattatactc atttatgaat gtttaattga atacaaaaaa     2760

aaatacttgt tatgtattca attacgggtt aaaatataga caagttgaaa aatttaataa     2820

aaaaataagt cctcagctct tatatattaa gctaccaact tagtatataa gccaaaactt     2880

aaatgtgcta ccaacacatc aagccgttag agaactctat ctatagcaat atttcaaatg     2940

taccgacata caagagaaac attaactata tatattcaat ttatgagatt atcttaacag     3000

atataaatgt aaattgcaat aagtaagatt tagaagttta tagcctttgt gtattggaag     3060

cagtacgcaa aggctttttt atttgataaa aattagaagt atatttattt tttcataatt     3120

aatttatgaa aatgaaaggg ggtgagcaaa gtgacagagg aaagcagtat cttatcaaat     3180

aacaaggtat tagcaatatc attattgact ttagcagtaa acattatgac ttttatagtg     3240

cttgtagcta agtagtacga aagggggagc tttaaaaagc tccttggaat acatagaatt     3300

cataaattaa tttatgaaaa gaagggcgta tatgaaaact tgtaaaaatt gcaaagagtt     3360

tattaaagat actgaaatat gcaaaataca ttcgttgatg attcatgata aaacagtagc     3420

aacctattgc agtaaataca atgagtcaag atgtttacat aaagggaaag tccaatgtat     3480

taattgttca aagatgaacc gatatggatg gtgtgccata aaaatgagat gttttacaga     3540

ggaagaacag aaaaaagaac gtacatgcat taaatattat gcaaggagct ttaaaaaagc     3600

tcatgtaaag aagagtaaaa agaaaaaata atttatttat taatttaata ttgagagtgc     3660

cgacacagta tgcactaaaa aatatatctg tggtgtagtg agccgataca aaaggatagt     3720

cactcgcatt ttcataatac atcttatgtt atgattatgt gtcggtggga cttcacgacg     3780

aaaacccaca ataaaaaaag agttcggggt agggttaagc atagttgagg caactaaaca     3840

atcaagctag gatatgcagt agcagaccgt aaggtcgttg tttaggtgtg ttgtaataca     3900

tacgctatta agatgtaaaa atacggatac caatgaaggg aaaagtataa tttttggatg     3960

tagtttgttt gttcatctat gggcaaacta cgtccaaagc cgtttccaaa tctgctaaaa     4020

agtatatcct ttctaaaatc aaagtcaagt atgaaatcat aaataaagtt taattttgaa     4080

gttattatga tattatgttt ttctattaaa ataaattaag tatatagaat agtttaataa     4140

tagtatatac ttaatgtgat aagtgtctga cagtgtcaca gaaaggatga ttgttatgga     4200

ttataagcgg ccggccagtg ggcaagttga aaaattcaca aaaatgtggt ataatatctt     4260

tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt tgaaaaaatt     4320

gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag tgtaccttgt     4380

acctacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg aatgaaacta     4440

tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga gtttaggacg     4500

gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag ctatacaata     4560

tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc tgactttaaa     4620

tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca tagaatggaa     4680

ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg gtcaaccttc     4740

gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat ttttactatg     4800

gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca agttcatcac     4860

gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga attgataaat     4920

agttaacttc aggtttgtct gtaactaaaa acaagtattt aagcaaaaac atcgtagaaa     4980

tacggtgttt tttgttaccc taagtttaaa ctcctttttg ataatctcat gaccaaaatc     5040

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     5100

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     5160

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     5220

ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac     5280

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     5340

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     5400

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     5460

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     5520

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     5580

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     5640

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     5700

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct     5760

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct     5820

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca     5880

atacgcaggg ccccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt     5940

ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa     6000

cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttct       6058


<210>  7
<211>  54
<212>  DNA
<213>  Bacillus subtilis

<400>  7
aagaagaagt gtgaaaaagc gcagctgaaa tagctgcgct tttttgtgtc ataa             54


<210>  8
<211>  45
<212>  DNA
<213>  Bacillus subtilis

<400>  8
ataatcaatc gtcccttcgt gtaaacgaag gggcgttttt tattt                       45


<210>  9
<211>  52
<212>  DNA
<213>  Lactococcus lactis

<400>  9
aatttataaa taaaaatcac cttttagagg tggttttttt atttataaat ta               52


<210>  10
<211>  83
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  10
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctcctc atg                                               83


<210>  11
<211>  85
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  11
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctct tcatg                                             85


<210>  12
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  12
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaagaccat catg                                              84


<210>  13
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  13
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctctaa tg                                                82


<210>  14
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  14
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctct aatg                                              84


<210>  15
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  15
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggaagacat aatg                                              84


<210>  16
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  16
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctcact tatg                                              84


<210>  17
<211>  86
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  17
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctca cttatg                                            86


<210>  18
<211>  85
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer Sequence for Expression Vector for Clostridia species

<400>  18
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaagactac ttatg                                             85


<210>  19
<211>  190
<212>  DNA
<213>  Artificial

<220>
<223>  Modified promoter for fdx gene of Clostridium autoethanogenum

<400>  19
ggccgctcac tatctgcgga acctgcctcc ttatctgata aaaaatattc gctgcatctt       60

tgacttgtta ttttctttca aatgcctaat ggaattgtga gcggataaca attaattatc      120

ttttaaaatt ataacaaatg tgataaaata caggggatga aaacattatc taaaaattaa      180

ggaggtgtta                                                             190


<210>  20
<211>  477
<212>  DNA
<213>  Artificial

<220>
<223>  Modified promoter for pfor gene of Clostridium authethanogenum

<400>  20
ggccgcaaaa tagttgataa taatgcagag ttataaacaa aggtgaaaag cattacttgt       60

attctttttt atatattatt ataaattaaa atgaagctgt attagaaaaa atacacacct      120

gtaatataaa attttaaatt aatttttaat tttttcaaaa tgtattttac atgtttagaa      180

ttttgatgta tattaaaata gtagaataca taagatactt aatttaatta aagatagtta      240

agtacttttc aatgtgcttt tttagatgtt taatacaaat ctttaattgt aaaagaaatg      300

ctgtactatt tactgtacta gtgacgggat taaactgtat taattataaa taaaaaataa      360

gtacagttgt ttaaaattat attttgtatt aaatctaata gtacgatgta agttatttta      420

tactattgct agtttaataa aaagatttaa ttatatactt gaaaaggaga ggaatat         477


<210>  21
<211>  562
<212>  DNA
<213>  Artificial

<220>
<223>  Modified promoter for wl gene of Clostridium autoethanogenum

<400>  21
ggccgcagat agtcataata gttccagaat agttcaattt agaaattaga ctaaacttca       60

aaatgtttgt taaatatata ccaaactagt atagatattt tttaaatact ggacttaaac      120

agtagtaatt tgcctaaaaa attttttcaa ttttttttaa aaaatccttt tcaagttgta      180

cattgttatg gtaatatgta attgaagaag ttatgtagta atattgtaaa cgtttcttga      240

tttttttaca tccatgtagt gcttaaaaaa ccaaaatatg tcacatgcaa ttgtatattt      300

caaataacaa tatttatttt ctcgttaaat tcacaaataa tttattaata atatcaataa      360

ccaagattat acttaaatgg atgtttattt tttaacactt ttatagtaaa tatatttatt      420

ttatgtagta aaaaggttat aattataatt gtatttatta caattaatta aaataaaaaa      480

tagggtttta ggtaaaatta agttatttta agaagtaatt acaataaaaa ttgaagttat      540

ttctttaagg agggaattat at                                               562


<210>  22
<211>  486
<212>  DNA
<213>  Artificial

<220>
<223>  Modifed promoter for pta gene of Clostridium autoethanogenum

<400>  22
ggccgcaata tgatatttat gtccattgtg aaagggatta tattcaacta ttattccagt       60

tacgttcata gaaattttcc tttctaaaat attttattcc atgtcaagaa ctctgtttat      120

ttcattaaag aactataagt acaaagtata aggcatttga aaaaataggc tagtatattg      180

attgattatt tattttaaaa tgcctaagtg aaatatatac atattataac aataaaataa      240

gtattagtgt aggattttta aatagagtat ctattttcag attaaatttt tgattatttg      300

atttacatta tataatattg agtaaagtat tgactagcaa aattttttga tactttaatt      360

tgtgaaattt cttatcaaaa gttatatttt tgaataattt ttattgaaaa atacaactaa      420

aaaggattat agtataagtg tgtgtaattt tgtgttaaat ttaaagggag gaaatgaaca      480

tgaaac                                                                 486


<210>  23
<211>  83
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter and ribosome binding site

<400>  23
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agatatacat atg                                               83


<210>  24
<211>  83
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  24
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctcctc atg                                               83


<210>  25
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  25
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctctaa tg                                                82


<210>  26
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  26
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggtctcact tatg                                              84


<210>  27
<211>  85
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  27
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctct tcatg                                             85


<210>  28
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  28
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctct aatg                                              84


<210>  29
<211>  86
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BsaI site

<400>  29
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaggtctca cttatg                                            86


<210>  30
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BbsI site

<400>  30
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaagaccat catg                                              84


<210>  31
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BbsI site

<400>  31
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg aggaagacat aatg                                              84


<210>  32
<211>  85
<212>  DNA
<213>  Artificial

<220>
<223>  Modified sequence for T7 promoter, ribosome binding site, and 
       BbsI site

<400>  32
taatacgact cactataggg acaccacaac ggtttccctc tagaaataat tttgtttaac       60

tttaagaagg agaagactac ttatg                                             85


