                               SEQUENCE LISTING

<110> ARBOR BIOTECHNOLOGIES, INC.
 
<120> COMPOSITIONS COMPRISING A NUCLEASE AND USES THEREOF

<130> A2186-7030WO

<140>
<141>

<150> 62/943,680
<151> 2019-12-04

<160> 20    

<170> PatentIn version 3.5

<210> 1
<211> 870
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Lys Val His Glu Ile Pro Arg Ser Gln Leu Leu Lys Ile Lys Gln 
1               5                   10                  15      


Tyr Glu Gly Ser Phe Val Glu Trp Tyr Arg Asp Leu Gln Glu Asp Arg 
            20                  25                  30          


Lys Lys Phe Ala Ser Leu Leu Phe Arg Trp Ala Ala Phe Gly Tyr Ala 
        35                  40                  45              


Ala Arg Glu Asp Asp Gly Ala Thr Tyr Ile Ser Pro Ser Gln Ala Leu 
    50                  55                  60                  


Leu Glu Arg Arg Leu Leu Leu Gly Asp Ala Glu Asp Val Ala Ile Lys 
65                  70                  75                  80  


Phe Leu Asp Val Leu Phe Lys Gly Gly Ala Pro Ser Ser Ser Cys Tyr 
                85                  90                  95      


Ser Leu Phe Tyr Glu Asp Phe Ala Leu Arg Asp Lys Ala Lys Tyr Ser 
            100                 105                 110         


Gly Ala Lys Arg Glu Phe Ile Glu Gly Leu Ala Thr Met Pro Leu Asp 
        115                 120                 125             


Lys Ile Ile Glu Arg Ile Arg Gln Asp Glu Gln Leu Ser Lys Ile Pro 
    130                 135                 140                 


Ala Glu Glu Trp Leu Ile Leu Gly Ala Glu Tyr Ser Pro Glu Glu Ile 
145                 150                 155                 160 


Trp Glu Gln Val Ala Pro Arg Ile Val Asn Val Asp Arg Ser Leu Gly 
                165                 170                 175     


Lys Gln Leu Arg Glu Arg Leu Gly Ile Lys Cys Arg Arg Pro His Asp 
            180                 185                 190         


Ala Gly Tyr Cys Lys Ile Leu Met Glu Val Val Ala Arg Gln Leu Arg 
        195                 200                 205             


Ser His Asn Glu Thr Tyr His Glu Tyr Leu Asn Gln Thr His Glu Met 
    210                 215                 220                 


Lys Thr Lys Val Ala Asn Asn Leu Thr Asn Glu Phe Asp Leu Val Cys 
225                 230                 235                 240 


Glu Phe Ala Glu Val Leu Glu Glu Lys Asn Tyr Gly Leu Gly Trp Tyr 
                245                 250                 255     


Val Leu Trp Gln Gly Val Lys Gln Ala Leu Lys Glu Gln Lys Lys Pro 
            260                 265                 270         


Thr Lys Ile Gln Ile Ala Val Asp Gln Leu Arg Gln Pro Lys Phe Ala 
        275                 280                 285             


Gly Leu Leu Thr Ala Lys Trp Arg Ala Leu Lys Gly Ala Tyr Asp Thr 
    290                 295                 300                 


Trp Lys Leu Lys Lys Arg Leu Glu Lys Arg Lys Ala Phe Pro Tyr Met 
305                 310                 315                 320 


Pro Asn Trp Asp Asn Asp Tyr Gln Ile Pro Val Gly Leu Thr Gly Leu 
                325                 330                 335     


Gly Val Phe Thr Leu Glu Val Lys Arg Thr Glu Val Val Val Asp Leu 
            340                 345                 350         


Lys Glu His Gly Lys Leu Phe Cys Ser His Ser His Tyr Phe Gly Asp 
        355                 360                 365             


Leu Thr Ala Glu Lys His Pro Ser Arg Tyr His Leu Lys Phe Arg His 
    370                 375                 380                 


Lys Leu Lys Leu Arg Lys Arg Asp Ser Arg Val Glu Pro Thr Ile Gly 
385                 390                 395                 400 


Pro Trp Ile Glu Ala Ala Leu Arg Glu Ile Thr Ile Gln Lys Lys Pro 
                405                 410                 415     


Asn Gly Val Phe Tyr Leu Gly Leu Pro Tyr Ala Leu Ser His Gly Ile 
            420                 425                 430         


Asp Asn Phe Gln Ile Ala Lys Arg Phe Phe Ser Ala Ala Lys Pro Asp 
        435                 440                 445             


Lys Glu Val Ile Asn Gly Leu Pro Ser Glu Met Val Val Gly Ala Ala 
    450                 455                 460                 


Asp Leu Asn Leu Ser Asn Ile Val Ala Pro Val Lys Ala Arg Ile Gly 
465                 470                 475                 480 


Lys Gly Leu Glu Gly Pro Leu His Ala Leu Asp Tyr Gly Tyr Gly Glu 
                485                 490                 495     


Leu Ile Asp Gly Pro Lys Ile Leu Thr Pro Asp Gly Pro Arg Cys Gly 
            500                 505                 510         


Glu Leu Ile Ser Leu Lys Arg Asp Ile Val Glu Ile Lys Ser Ala Ile 
        515                 520                 525             


Lys Glu Phe Lys Ala Cys Gln Arg Glu Gly Leu Thr Met Ser Glu Glu 
    530                 535                 540                 


Thr Thr Thr Trp Leu Ser Glu Val Glu Ser Pro Ser Asp Ser Pro Arg 
545                 550                 555                 560 


Cys Met Ile Gln Ser Arg Ile Ala Asp Thr Ser Arg Arg Leu Asn Ser 
                565                 570                 575     


Phe Lys Tyr Gln Met Asn Lys Glu Gly Tyr Gln Asp Leu Ala Glu Ala 
            580                 585                 590         


Leu Arg Leu Leu Asp Ala Met Asp Ser Tyr Asn Ser Leu Leu Glu Ser 
        595                 600                 605             


Tyr Gln Arg Met His Leu Ser Pro Gly Glu Gln Ser Pro Lys Glu Ala 
    610                 615                 620                 


Lys Phe Asp Thr Lys Arg Ala Ser Phe Arg Asp Leu Leu Arg Arg Arg 
625                 630                 635                 640 


Val Ala His Thr Ile Val Glu Tyr Phe Asp Asp Cys Asp Ile Val Phe 
                645                 650                 655     


Phe Glu Asp Leu Asp Gly Pro Ser Asp Ser Asp Ser Arg Asn Asn Ala 
            660                 665                 670         


Leu Val Lys Leu Leu Ser Pro Arg Thr Leu Leu Leu Tyr Ile Arg Gln 
        675                 680                 685             


Ala Leu Glu Lys Arg Gly Ile Gly Met Val Glu Val Ala Lys Asp Gly 
    690                 695                 700                 


Thr Ser Gln Asn Asn Pro Ile Ser Gly His Val Gly Trp Arg Asn Lys 
705                 710                 715                 720 


Gln Asn Lys Ser Glu Ile Tyr Phe Tyr Glu Asp Lys Glu Leu Leu Val 
                725                 730                 735     


Met Asp Ala Asp Glu Val Gly Ala Met Asn Ile Leu Cys Arg Gly Leu 
            740                 745                 750         


Asn His Ser Val Cys Pro Tyr Ser Phe Val Thr Lys Ala Pro Glu Lys 
        755                 760                 765             


Lys Asn Asp Glu Lys Lys Glu Gly Asp Tyr Gly Lys Arg Val Lys Arg 
    770                 775                 780                 


Phe Leu Lys Asp Arg Tyr Gly Ser Ser Asn Val Arg Phe Leu Val Ala 
785                 790                 795                 800 


Ser Met Gly Phe Val Thr Val Thr Thr Lys Arg Pro Lys Asp Ala Leu 
                805                 810                 815     


Val Gly Lys Arg Leu Tyr Tyr His Gly Gly Glu Leu Val Thr His Asp 
            820                 825                 830         


Leu His Asn Arg Met Lys Asp Glu Ile Lys Tyr Leu Val Glu Lys Glu 
        835                 840                 845             


Val Leu Ala Arg Arg Val Ser Leu Ser Asp Ser Thr Ile Lys Ser Tyr 
    850                 855                 860                 


Lys Ser Phe Ala His Val 
865                 870 


<210> 2
<211> 870
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Lys Val His Glu Ile Pro Arg Ser Gln Leu Leu Lys Ile Lys Gln 
1               5                   10                  15      


Tyr Glu Gly Ser Phe Val Glu Trp Tyr Arg Asp Leu Gln Glu Asp Arg 
            20                  25                  30          


Lys Lys Phe Ala Ser Leu Leu Phe Arg Trp Ala Ala Phe Gly Tyr Ala 
        35                  40                  45              


Ala Arg Glu Asp Asp Gly Ala Thr Tyr Ile Ser Pro Ser Gln Ala Leu 
    50                  55                  60                  


Leu Glu Arg Arg Leu Leu Leu Gly Asp Ala Glu Asp Val Ala Ile Lys 
65                  70                  75                  80  


Phe Leu Asp Val Leu Phe Lys Gly Gly Ala Pro Ser Ser Ser Cys Tyr 
                85                  90                  95      


Ser Leu Phe Tyr Glu Asp Phe Ala Leu Arg Asp Lys Ala Lys Tyr Ser 
            100                 105                 110         


Gly Ala Lys Arg Glu Phe Ile Glu Gly Leu Ala Thr Met Pro Leu Asp 
        115                 120                 125             


Lys Ile Ile Glu Arg Ile Arg Gln Asp Glu Gln Leu Ser Lys Ile Pro 
    130                 135                 140                 


Ala Glu Glu Trp Leu Ile Leu Gly Ala Glu Tyr Ser Pro Glu Glu Ile 
145                 150                 155                 160 


Trp Glu Gln Val Ala Pro Arg Ile Val Asn Val Asp Arg Ser Leu Gly 
                165                 170                 175     


Lys Gln Leu Arg Glu Arg Leu Gly Ile Lys Cys Arg Arg Pro His Asp 
            180                 185                 190         


Ala Gly Tyr Cys Lys Ile Leu Met Glu Val Val Ala Arg Gln Leu Arg 
        195                 200                 205             


Ser His Asn Glu Thr Tyr His Glu Tyr Leu Asn Gln Thr His Glu Met 
    210                 215                 220                 


Lys Thr Lys Val Ala Asn Asn Leu Thr Asn Glu Phe Asp Leu Val Cys 
225                 230                 235                 240 


Glu Phe Ala Glu Val Leu Glu Glu Lys Asn Tyr Gly Leu Gly Trp Tyr 
                245                 250                 255     


Val Leu Trp Gln Gly Val Lys Gln Ala Leu Lys Glu Gln Lys Lys Pro 
            260                 265                 270         


Thr Lys Ile Gln Ile Ala Val Asp Gln Leu Arg Gln Pro Lys Phe Ala 
        275                 280                 285             


Gly Leu Leu Thr Ala Lys Trp Arg Ala Leu Lys Gly Ala Tyr Asp Thr 
    290                 295                 300                 


Trp Lys Leu Lys Lys Arg Leu Glu Lys Arg Lys Ala Phe Pro Tyr Met 
305                 310                 315                 320 


Pro Asn Trp Asp Asn Asp Tyr Gln Ile Pro Val Gly Leu Thr Gly Leu 
                325                 330                 335     


Gly Val Phe Thr Leu Glu Val Lys Arg Thr Glu Val Val Val Asp Leu 
            340                 345                 350         


Lys Glu His Gly Lys Leu Phe Cys Ser His Ser His Tyr Phe Gly Asp 
        355                 360                 365             


Leu Thr Ala Glu Lys His Pro Ser Arg Tyr His Leu Lys Phe Arg His 
    370                 375                 380                 


Lys Leu Lys Leu Arg Lys Arg Asp Ser Arg Val Glu Pro Thr Ile Gly 
385                 390                 395                 400 


Pro Trp Ile Glu Ala Ala Leu Arg Glu Ile Thr Ile Gln Lys Lys Pro 
                405                 410                 415     


Asn Gly Val Phe Tyr Leu Gly Leu Pro Tyr Ala Leu Ser His Gly Ile 
            420                 425                 430         


Asp Asn Phe Gln Ile Ala Lys Arg Phe Phe Ser Ala Ala Lys Pro Asp 
        435                 440                 445             


Lys Glu Val Ile Asn Gly Leu Pro Ser Glu Met Val Val Gly Ala Ala 
    450                 455                 460                 


Ala Leu Asn Leu Ser Asn Ile Val Ala Pro Val Lys Ala Arg Ile Gly 
465                 470                 475                 480 


Lys Gly Leu Glu Gly Pro Leu His Ala Leu Asp Tyr Gly Tyr Gly Glu 
                485                 490                 495     


Leu Ile Asp Gly Pro Lys Ile Leu Thr Pro Asp Gly Pro Arg Cys Gly 
            500                 505                 510         


Glu Leu Ile Ser Leu Lys Arg Asp Ile Val Glu Ile Lys Ser Ala Ile 
        515                 520                 525             


Lys Glu Phe Lys Ala Cys Gln Arg Glu Gly Leu Thr Met Ser Glu Glu 
    530                 535                 540                 


Thr Thr Thr Trp Leu Ser Glu Val Glu Ser Pro Ser Asp Ser Pro Arg 
545                 550                 555                 560 


Cys Met Ile Gln Ser Arg Ile Ala Asp Thr Ser Arg Arg Leu Asn Ser 
                565                 570                 575     


Phe Lys Tyr Gln Met Asn Lys Glu Gly Tyr Gln Asp Leu Ala Glu Ala 
            580                 585                 590         


Leu Arg Leu Leu Asp Ala Met Asp Ser Tyr Asn Ser Leu Leu Glu Ser 
        595                 600                 605             


Tyr Gln Arg Met His Leu Ser Pro Gly Glu Gln Ser Pro Lys Glu Ala 
    610                 615                 620                 


Lys Phe Asp Thr Lys Arg Ala Ser Phe Arg Asp Leu Leu Arg Arg Arg 
625                 630                 635                 640 


Val Ala His Thr Ile Val Glu Tyr Phe Asp Asp Cys Asp Ile Val Phe 
                645                 650                 655     


Phe Glu Asp Leu Asp Gly Pro Ser Asp Ser Asp Ser Arg Asn Asn Ala 
            660                 665                 670         


Leu Val Lys Leu Leu Ser Pro Arg Thr Leu Leu Leu Tyr Ile Arg Gln 
        675                 680                 685             


Ala Leu Glu Lys Arg Gly Ile Gly Met Val Glu Val Ala Lys Asp Gly 
    690                 695                 700                 


Thr Ser Gln Asn Asn Pro Ile Ser Gly His Val Gly Trp Arg Asn Lys 
705                 710                 715                 720 


Gln Asn Lys Ser Glu Ile Tyr Phe Tyr Glu Asp Lys Glu Leu Leu Val 
                725                 730                 735     


Met Asp Ala Asp Glu Val Gly Ala Met Asn Ile Leu Cys Arg Gly Leu 
            740                 745                 750         


Asn His Ser Val Cys Pro Tyr Ser Phe Val Thr Lys Ala Pro Glu Lys 
        755                 760                 765             


Lys Asn Asp Glu Lys Lys Glu Gly Asp Tyr Gly Lys Arg Val Lys Arg 
    770                 775                 780                 


Phe Leu Lys Asp Arg Tyr Gly Ser Ser Asn Val Arg Phe Leu Val Ala 
785                 790                 795                 800 


Ser Met Gly Phe Val Thr Val Thr Thr Lys Arg Pro Lys Asp Ala Leu 
                805                 810                 815     


Val Gly Lys Arg Leu Tyr Tyr His Gly Gly Glu Leu Val Thr His Asp 
            820                 825                 830         


Leu His Asn Arg Met Lys Asp Glu Ile Lys Tyr Leu Val Glu Lys Glu 
        835                 840                 845             


Val Leu Ala Arg Arg Val Ser Leu Ser Asp Ser Thr Ile Lys Ser Tyr 
    850                 855                 860                 


Lys Ser Phe Ala His Val 
865                 870 


<210> 3
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
gtgctggccg ctctcgctag agggaggtca gagcac                                 36


<210> 4
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
gtgctctgac ctccctctag cgagagcggc cagcac                                 36


<210> 5
<211> 140
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Target sequence

<400> 5
aaacttagga cgacaaagtg tcgccttcca gttcggtgat atacgggatc tctttctcaa       60

acagttttgc accttccgtc aatgccgtca tggatccgtg gtgatggtga tggtgacctt      120

ggtcaaatcg gtgtttgttt                                                  140


<210> 6
<211> 108
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 6
gtgctggccg ctctcgctag agggaggtca gagcacacgg cattgacgga aggtgcaaaa       60

ctgtttgaga aagtgctggc cgctctcgct agagggaggt cagagcac                   108


<210> 7
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
acggcattga cggaaggtgc aaaactgttt gagaaa                                 36


<210> 8
<211> 140
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Target sequence

<400> 8
aaacttagga cgacaaagtg cagatgtatt tcgctttaat ggtacccgtg gtcgcgtcac       60

cggtaccctc gcctttaatg ataaatttca taccttcgac gtcgccttcc agttcggtga      120

ggtcaaatcg gtgtttgttt                                                  140


<210> 9
<211> 108
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 9
gtgctggccg ctctcgctag agggaggtca gagcacaaat ttatcattaa aggcgagggt       60

accggtgacg cggtgctggc cgctctcgct agagggaggt cagagcac                   108


<210> 10
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
aaatttatca ttaaaggcga gggtaccggt gacgcg                                 36


<210> 11
<211> 140
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Target sequence

<400> 11
aaacttagga cgacaaagtg aaactgtttg agaaagagat cccgtatatc accgaactgg       60

aaggcgacgt cgaaggtatg aaatttatca ttaaaggcga gggtaccggt gacgcgacca      120

ggtcaaatcg gtgtttgttt                                                  140


<210> 12
<211> 108
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 12
gtgctggccg ctctcgctag agggaggtca gagcacataa atttcatacc ttcgacgtcg       60

ccttccagtt cggtgctggc cgctctcgct agagggaggt cagagcac                   108


<210> 13
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
ataaatttca taccttcgac gtcgccttcc agttcg                                 36


<210> 14
<211> 140
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Target sequence

<400> 14
aaacttagga cgacaaagtg aagtacccga gccacatcaa ggatttcttt aagagcgcca       60

tgccggaagg ttatacccaa gagcgtacca tcagcttcga aggcgacggc gtgtacaaga      120

ggtcaaatcg gtgtttgttt                                                  140


<210> 15
<211> 108
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 15
gtgctggccg ctctcgctag agggaggtca gagcacgtac gctcttgggt ataaccttcc       60

ggcatggcgc tcgtgctggc cgctctcgct agagggaggt cagagcac                   108


<210> 16
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
gtacgctctt gggtataacc ttccggcatg gcgctc                                 36


<210> 17
<211> 139
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Target sequence


<220>
<221> modified_base
<222> (50)..(57)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (94)..(101)
<223> a, c, t, g, unknown or other

<400> 17
tccatgtctc gttatacgct gtggttcgcc aacgcactca gcaactactn nnnnnnnccg       60

aacctgttca ataagtgtcc tgtttctata ccannnnnnn nactactctc agcattgaca      120

gctagctcag tcctaggta                                                   139


<210> 18
<211> 108
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 18
gtgctggccg ctctcgctag agggaggtca gagcactggt atagaaacag gacacttatt       60

gaacaggttc gggtgctggc cgctctcgct agagggaggt cagagcac                   108


<210> 19
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
tggtatagaa acaggacact tattgaacag gttcgg                                 36


<210> 20
<211> 36
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
gugcuggccg cucucgcuag agggagguca gagcac                                 36


